AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0555860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0555860 MtrunA17_Chr4g0042141 95.161 124 4 1 1 124 1 122 1.14e-82 237
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGTTCCATTGAAGGATT+TGG 0.262218 4_1:-53879775 None:intergenic
TTCGAATCTATGAAGAGATT+TGG 0.340535 4_1:-53879810 None:intergenic
AAATGCGCGGTGGCTTCTGC+TGG 0.379019 4_1:+53880087 Msa0555860:CDS
CCGGATTGGAGGCCTTCGTT+GGG 0.381706 4_1:+53880033 Msa0555860:CDS
TGAAGGTAGGTGGCTAGTTC+CGG 0.383752 4_1:+53879951 Msa0555860:CDS
GCCTTCGTTGGGGTCGATAT+CGG 0.394917 4_1:+53880044 Msa0555860:CDS
GTGCTCGTGGTGGTGCTGGT+AGG 0.397018 4_1:+53880133 Msa0555860:CDS
ATCATTTCCAAATCCTTCAA+TGG 0.413195 4_1:+53879768 Msa0555860:five_prime_UTR
GGAAGGTTGGTGCGCCGGAT+TGG 0.415044 4_1:+53880019 Msa0555860:CDS
GCCACAGCAACAACGGGAGC+CGG 0.420519 4_1:-53879970 None:intergenic
ACGGAAGCCACAGCAACAAC+GGG 0.431180 4_1:-53879976 None:intergenic
GTGGCTTCTGCTGGAAGGGA+GGG 0.431428 4_1:+53880096 Msa0555860:CDS
GCCGGATTGGAGGCCTTCGT+TGG 0.439790 4_1:+53880032 Msa0555860:CDS
CACGTCGGAATCTTTAGTTC+CGG 0.441249 4_1:+53879921 Msa0555860:CDS
GATAACCTTCGCTGTAACTC+CGG 0.444405 4_1:-53880164 None:intergenic
AGTGGTGCTCGTGGTGGTGC+TGG 0.453757 4_1:+53880129 Msa0555860:CDS
TCACAAATTTAAAACATGAA+TGG 0.460670 4_1:-53881180 None:intergenic
TCATTCTTCGGAGCCGGATG+AGG 0.461122 4_1:+53879857 Msa0555860:exon
CGCGGTGGCTTCTGCTGGAA+GGG 0.461344 4_1:+53880092 Msa0555860:CDS
ATTTAAAACATGAATGGCGT+TGG 0.467156 4_1:-53881174 None:intergenic
CCCAACGAAGGCCTCCAATC+CGG 0.470447 4_1:-53880033 None:intergenic
AGGTTGGTGCGCCGGATTGG+AGG 0.471997 4_1:+53880022 Msa0555860:CDS
ATGAAGAGATTTGGCGGTGG+AGG 0.476270 4_1:-53879801 None:intergenic
TGCTCGTGGTGGTGCTGGTA+GGG 0.480254 4_1:+53880134 Msa0555860:CDS
TGGCTTCTGCTGGAAGGGAG+GGG 0.495000 4_1:+53880097 Msa0555860:CDS
TCCGGCTCCCGTTGTTGCTG+TGG 0.501608 4_1:+53879969 Msa0555860:CDS
GGTGGCTTCTGCTGGAAGGG+AGG 0.502321 4_1:+53880095 Msa0555860:CDS
AGGGAAATAAGTTCCATTGA+AGG 0.502430 4_1:-53879781 None:intergenic
CACGGAAGCCACAGCAACAA+CGG 0.514609 4_1:-53879977 None:intergenic
ATTACTTTAATTTAATTACA+AGG 0.521233 4_1:-53881267 None:intergenic
TTAGTTCCGGCGGAGGATGA+AGG 0.522461 4_1:+53879934 Msa0555860:CDS
AGTTACAGCGAAGGTTATCA+CGG 0.523326 4_1:+53880168 Msa0555860:CDS
GGAATCTTTAGTTCCGGCGG+AGG 0.524866 4_1:+53879927 Msa0555860:CDS
TAAGAGGAAGGTTGGTGCGC+CGG 0.525975 4_1:+53880014 Msa0555860:CDS
AAAACGGTGGAGAGAGGGAA+AGG 0.531145 4_1:-53879835 None:intergenic
TGAGGCGTCAAAATCGTAAG+AGG 0.531246 4_1:+53879998 Msa0555860:CDS
GCGCGGTGGCTTCTGCTGGA+AGG 0.540922 4_1:+53880091 Msa0555860:CDS
GAAGCCACCGCGCATTTCTG+CGG 0.542157 4_1:-53880081 None:intergenic
CGCATCGCCGCGGCACACGT+CGG 0.548898 4_1:+53879906 Msa0555860:CDS
TGAAGAGATTTGGCGGTGGA+GGG 0.553533 4_1:-53879800 None:intergenic
CAAAATCGTAAGAGGAAGGT+TGG 0.559795 4_1:+53880006 Msa0555860:CDS
GAATGAAAACGGTGGAGAGA+GGG 0.560861 4_1:-53879840 None:intergenic
CACCTACCTTCATCCTCCGC+CGG 0.561228 4_1:-53879940 None:intergenic
CCGGCTCCGAAGAATGAAAA+CGG 0.562190 4_1:-53879851 None:intergenic
AGGGAGGGGAAGAAGAAGAG+TGG 0.568872 4_1:+53880111 Msa0555860:CDS
GAATCTATGAAGAGATTTGG+CGG 0.578566 4_1:-53879807 None:intergenic
GCTAGGTGAAACTGTTGCTA+AGG 0.597624 4_1:+53881239 Msa0555860:three_prime_UTR
AGAATGAAAACGGTGGAGAG+AGG 0.606074 4_1:-53879841 None:intergenic
GTCGGAATCTTTAGTTCCGG+CGG 0.609641 4_1:+53879924 Msa0555860:CDS
TCCGATATCGACCCCAACGA+AGG 0.613627 4_1:-53880045 None:intergenic
GTTACAGCGAAGGTTATCAC+GGG 0.620207 4_1:+53880169 Msa0555860:CDS
TTTCTTGTTGCGCATCGCCG+CGG 0.624650 4_1:+53879896 Msa0555860:CDS
AAAGATTCCGACGTGTGCCG+CGG 0.627143 4_1:-53879913 None:intergenic
CGGATTGGAGGCCTTCGTTG+GGG 0.628555 4_1:+53880034 Msa0555860:CDS
AATGGCGTTGGAGAGAATGC+TGG 0.629506 4_1:-53881162 None:intergenic
GTGCTGGTAGGGTTTATCAC+CGG 0.634833 4_1:+53880145 Msa0555860:CDS
GCGTCAAAATCGTAAGAGGA+AGG 0.639456 4_1:+53880002 Msa0555860:CDS
CGGCGGAGGATGAAGGTAGG+TGG 0.643672 4_1:+53879941 Msa0555860:CDS
AAGAAGAAGAGTGGTGCTCG+TGG 0.645513 4_1:+53880120 Msa0555860:CDS
TTCGTTGGGGTCGATATCGG+AGG 0.655426 4_1:+53880047 Msa0555860:CDS
AGCGCCGCAGAAATGCGCGG+TGG 0.664042 4_1:+53880077 Msa0555860:CDS
ATATATGCTAGTATGCTGCT+AGG 0.664065 4_1:+53881222 Msa0555860:three_prime_UTR
ACTTTAATTTAATTACAAGG+TGG 0.668387 4_1:-53881264 None:intergenic
TTCCGGCGGAGGATGAAGGT+AGG 0.674632 4_1:+53879938 Msa0555860:CDS
TATAGCGCCGCAGAAATGCG+CGG 0.685952 4_1:+53880074 Msa0555860:CDS
TCTATGAAGAGATTTGGCGG+TGG 0.695619 4_1:-53879804 None:intergenic
GCTCCGAAGAATGAAAACGG+TGG 0.707320 4_1:-53879848 None:intergenic
AAGAAGAGTGGTGCTCGTGG+TGG 0.707624 4_1:+53880123 Msa0555860:CDS
TATCACCGGAGTTACAGCGA+AGG 0.715111 4_1:+53880159 Msa0555860:CDS
TTGTTGCTGTGGCTTCCGTG+AGG 0.746599 4_1:+53879980 Msa0555860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATTATATAAATATACA+AGG + chr4_1:53880252-53880271 Msa0555860:intron 10.0%
!!! TATTTTCTTTCATTAATTAT+AGG - chr4_1:53880514-53880533 None:intergenic 10.0%
!!! ATTACTTTAATTTAATTACA+AGG - chr4_1:53881270-53881289 None:intergenic 10.0%
!! AAGAATTATATAAATATACA+AGG + chr4_1:53880252-53880271 Msa0555860:intron 10.0%
!!! TATTTTCTTTCATTAATTAT+AGG - chr4_1:53880514-53880533 None:intergenic 10.0%
!!! ATTACTTTAATTTAATTACA+AGG - chr4_1:53881270-53881289 None:intergenic 10.0%
!! TAATGAAAGAAAATATGATA+TGG + chr4_1:53880519-53880538 Msa0555860:intron 15.0%
!! AATGAAAGAAAATATGATAT+GGG + chr4_1:53880520-53880539 Msa0555860:intron 15.0%
!!! AAATAAAATGAAAGTATGTT+AGG + chr4_1:53880618-53880637 Msa0555860:intron 15.0%
!! TAATGAAAGAAAATATGATA+TGG + chr4_1:53880519-53880538 Msa0555860:intron 15.0%
!! AATGAAAGAAAATATGATAT+GGG + chr4_1:53880520-53880539 Msa0555860:intron 15.0%
!!! AAATAAAATGAAAGTATGTT+AGG + chr4_1:53880618-53880637 Msa0555860:intron 15.0%
!! AACTTATAAGTCTTCTTATT+AGG + chr4_1:53880466-53880485 Msa0555860:intron 20.0%
!! TATAATAGTACAAAACATAC+TGG + chr4_1:53880559-53880578 Msa0555860:intron 20.0%
!! AAAAAATCAATCAAATGATG+TGG + chr4_1:53880798-53880817 Msa0555860:intron 20.0%
!!! GAATTGAACTTTTTTTTTGA+AGG - chr4_1:53880848-53880867 None:intergenic 20.0%
!! CATAAACAACTATTCAATAT+TGG - chr4_1:53880952-53880971 None:intergenic 20.0%
!!! TATTGTCTTTTGTTTTTGTT+TGG + chr4_1:53880976-53880995 Msa0555860:intron 20.0%
!!! ATTGTCTTTTGTTTTTGTTT+GGG + chr4_1:53880977-53880996 Msa0555860:intron 20.0%
!!! TCACAAATTTAAAACATGAA+TGG - chr4_1:53881183-53881202 None:intergenic 20.0%
!!! ACTTTAATTTAATTACAAGG+TGG - chr4_1:53881267-53881286 None:intergenic 20.0%
!! AACTTATAAGTCTTCTTATT+AGG + chr4_1:53880466-53880485 Msa0555860:intron 20.0%
!! TATAATAGTACAAAACATAC+TGG + chr4_1:53880559-53880578 Msa0555860:intron 20.0%
!! AAAAAATCAATCAAATGATG+TGG + chr4_1:53880798-53880817 Msa0555860:intron 20.0%
!!! GAATTGAACTTTTTTTTTGA+AGG - chr4_1:53880848-53880867 None:intergenic 20.0%
!! CATAAACAACTATTCAATAT+TGG - chr4_1:53880952-53880971 None:intergenic 20.0%
!!! TATTGTCTTTTGTTTTTGTT+TGG + chr4_1:53880976-53880995 Msa0555860:intron 20.0%
!!! ATTGTCTTTTGTTTTTGTTT+GGG + chr4_1:53880977-53880996 Msa0555860:intron 20.0%
!!! TCACAAATTTAAAACATGAA+TGG - chr4_1:53881183-53881202 None:intergenic 20.0%
!!! ACTTTAATTTAATTACAAGG+TGG - chr4_1:53881267-53881286 None:intergenic 20.0%
!! TGATTTTGTTGAGAATTTAG+GGG - chr4_1:53879724-53879743 None:intergenic 25.0%
!! GTGATTTTGTTGAGAATTTA+GGG - chr4_1:53879725-53879744 None:intergenic 25.0%
!! TGTGATTTTGTTGAGAATTT+AGG - chr4_1:53879726-53879745 None:intergenic 25.0%
! TATACAAGGATAATGATCTT+TGG + chr4_1:53880266-53880285 Msa0555860:intron 25.0%
!! AATCATTTTCTGATCAAGTT+TGG + chr4_1:53880340-53880359 Msa0555860:intron 25.0%
! TATTATAACATAACATGCAC+TGG - chr4_1:53880546-53880565 None:intergenic 25.0%
! CTTCAACAACAAAACAAATA+AGG + chr4_1:53880726-53880745 Msa0555860:intron 25.0%
! ACAACAAAACAAATAAGGAT+AGG + chr4_1:53880731-53880750 Msa0555860:intron 25.0%
! AAAAAAGTTCAATTCGTACT+AGG + chr4_1:53880853-53880872 Msa0555860:intron 25.0%
!! TTACTTTGTCATTTGTTAAG+TGG + chr4_1:53880896-53880915 Msa0555860:intron 25.0%
!!! GATTTTTGGTATAAAATTGC+AGG + chr4_1:53881115-53881134 Msa0555860:intron 25.0%
! CTCAAAACAAAACAAAAAAG+TGG - chr4_1:53881299-53881318 None:intergenic 25.0%
!! TGATTTTGTTGAGAATTTAG+GGG - chr4_1:53879724-53879743 None:intergenic 25.0%
!! GTGATTTTGTTGAGAATTTA+GGG - chr4_1:53879725-53879744 None:intergenic 25.0%
!! TGTGATTTTGTTGAGAATTT+AGG - chr4_1:53879726-53879745 None:intergenic 25.0%
! TATACAAGGATAATGATCTT+TGG + chr4_1:53880266-53880285 Msa0555860:intron 25.0%
!! AATCATTTTCTGATCAAGTT+TGG + chr4_1:53880340-53880359 Msa0555860:intron 25.0%
! TATTATAACATAACATGCAC+TGG - chr4_1:53880546-53880565 None:intergenic 25.0%
! CTTCAACAACAAAACAAATA+AGG + chr4_1:53880726-53880745 Msa0555860:intron 25.0%
! ACAACAAAACAAATAAGGAT+AGG + chr4_1:53880731-53880750 Msa0555860:intron 25.0%
! AAAAAAGTTCAATTCGTACT+AGG + chr4_1:53880853-53880872 Msa0555860:intron 25.0%
!! TTACTTTGTCATTTGTTAAG+TGG + chr4_1:53880896-53880915 Msa0555860:intron 25.0%
!!! GATTTTTGGTATAAAATTGC+AGG + chr4_1:53881115-53881134 Msa0555860:intron 25.0%
! CTCAAAACAAAACAAAAAAG+TGG - chr4_1:53881299-53881318 None:intergenic 25.0%
GGAAATGATGAGAGAAAAAA+TGG - chr4_1:53879757-53879776 None:intergenic 30.0%
ATCATTTCCAAATCCTTCAA+TGG + chr4_1:53879768-53879787 Msa0555860:five_prime_UTR 30.0%
ATAAGTTCCATTGAAGGATT+TGG - chr4_1:53879778-53879797 None:intergenic 30.0%
TTCGAATCTATGAAGAGATT+TGG - chr4_1:53879813-53879832 None:intergenic 30.0%
CCGTTAAAACTCAAAAACTA+GGG - chr4_1:53880210-53880229 None:intergenic 30.0%
!!! CCCTAGTTTTTGAGTTTTAA+CGG + chr4_1:53880207-53880226 Msa0555860:intron 30.0%
TCCGTTAAAACTCAAAAACT+AGG - chr4_1:53880211-53880230 None:intergenic 30.0%
CATGAAATTACACGAATGAT+TGG + chr4_1:53880390-53880409 Msa0555860:intron 30.0%
ATATCACTATTGCTTCAACA+AGG + chr4_1:53880644-53880663 Msa0555860:intron 30.0%
CGTGTATACTTTCTATTGAT+AGG - chr4_1:53880700-53880719 None:intergenic 30.0%
! TCATTTGTTAAGTGGTTAAG+TGG + chr4_1:53880904-53880923 Msa0555860:intron 30.0%
! TCACTAACAGACTTGATTTT+TGG + chr4_1:53881101-53881120 Msa0555860:intron 30.0%
! ATTTAAAACATGAATGGCGT+TGG - chr4_1:53881177-53881196 None:intergenic 30.0%
ACAAAACAAAAAAGTGGTAG+AGG - chr4_1:53881293-53881312 None:intergenic 30.0%
! GTTGATTTAGAAGCAAAAAG+TGG - chr4_1:53881326-53881345 None:intergenic 30.0%
GGAAATGATGAGAGAAAAAA+TGG - chr4_1:53879757-53879776 None:intergenic 30.0%
ATCATTTCCAAATCCTTCAA+TGG + chr4_1:53879768-53879787 Msa0555860:five_prime_UTR 30.0%
ATAAGTTCCATTGAAGGATT+TGG - chr4_1:53879778-53879797 None:intergenic 30.0%
TTCGAATCTATGAAGAGATT+TGG - chr4_1:53879813-53879832 None:intergenic 30.0%
CCGTTAAAACTCAAAAACTA+GGG - chr4_1:53880210-53880229 None:intergenic 30.0%
!!! CCCTAGTTTTTGAGTTTTAA+CGG + chr4_1:53880207-53880226 Msa0555860:intron 30.0%
TCCGTTAAAACTCAAAAACT+AGG - chr4_1:53880211-53880230 None:intergenic 30.0%
CATGAAATTACACGAATGAT+TGG + chr4_1:53880390-53880409 Msa0555860:intron 30.0%
ATATCACTATTGCTTCAACA+AGG + chr4_1:53880644-53880663 Msa0555860:intron 30.0%
CGTGTATACTTTCTATTGAT+AGG - chr4_1:53880700-53880719 None:intergenic 30.0%
! TCATTTGTTAAGTGGTTAAG+TGG + chr4_1:53880904-53880923 Msa0555860:intron 30.0%
! TCACTAACAGACTTGATTTT+TGG + chr4_1:53881101-53881120 Msa0555860:intron 30.0%
! ATTTAAAACATGAATGGCGT+TGG - chr4_1:53881177-53881196 None:intergenic 30.0%
ACAAAACAAAAAAGTGGTAG+AGG - chr4_1:53881293-53881312 None:intergenic 30.0%
! GTTGATTTAGAAGCAAAAAG+TGG - chr4_1:53881326-53881345 None:intergenic 30.0%
AGGGAAATAAGTTCCATTGA+AGG - chr4_1:53879784-53879803 None:intergenic 35.0%
GAATCTATGAAGAGATTTGG+CGG - chr4_1:53879810-53879829 None:intergenic 35.0%
ATTCCAAGAAAAACCTCATC+CGG - chr4_1:53879873-53879892 None:intergenic 35.0%
! CATTTTCTGATCAAGTTTGG+AGG + chr4_1:53880343-53880362 Msa0555860:intron 35.0%
! ACGAATGATTGGTCGTTATT+AGG + chr4_1:53880401-53880420 Msa0555860:intron 35.0%
ATATATGCTAGTATGCTGCT+AGG + chr4_1:53881222-53881241 Msa0555860:three_prime_UTR 35.0%
AGGGAAATAAGTTCCATTGA+AGG - chr4_1:53879784-53879803 None:intergenic 35.0%
GAATCTATGAAGAGATTTGG+CGG - chr4_1:53879810-53879829 None:intergenic 35.0%
ATTCCAAGAAAAACCTCATC+CGG - chr4_1:53879873-53879892 None:intergenic 35.0%
! CATTTTCTGATCAAGTTTGG+AGG + chr4_1:53880343-53880362 Msa0555860:intron 35.0%
! ACGAATGATTGGTCGTTATT+AGG + chr4_1:53880401-53880420 Msa0555860:intron 35.0%
ATATATGCTAGTATGCTGCT+AGG + chr4_1:53881222-53881241 Msa0555860:three_prime_UTR 35.0%
! TCTCCACCGTTTTCATTCTT+CGG + chr4_1:53879845-53879864 Msa0555860:five_prime_UTR 40.0%
CAAAATCGTAAGAGGAAGGT+TGG + chr4_1:53880006-53880025 Msa0555860:CDS 40.0%
AGTTACAGCGAAGGTTATCA+CGG + chr4_1:53880168-53880187 Msa0555860:CDS 40.0%
GGTCAACTGCATAATGAACA+AGG - chr4_1:53880679-53880698 None:intergenic 40.0%
! TCTCCACCGTTTTCATTCTT+CGG + chr4_1:53879845-53879864 Msa0555860:five_prime_UTR 40.0%
CAAAATCGTAAGAGGAAGGT+TGG + chr4_1:53880006-53880025 Msa0555860:CDS 40.0%
AGTTACAGCGAAGGTTATCA+CGG + chr4_1:53880168-53880187 Msa0555860:CDS 40.0%
GGTCAACTGCATAATGAACA+AGG - chr4_1:53880679-53880698 None:intergenic 40.0%
TCTATGAAGAGATTTGGCGG+TGG - chr4_1:53879807-53879826 None:intergenic 45.0%
GAATGAAAACGGTGGAGAGA+GGG - chr4_1:53879843-53879862 None:intergenic 45.0%
AGAATGAAAACGGTGGAGAG+AGG - chr4_1:53879844-53879863 None:intergenic 45.0%
! CACGTCGGAATCTTTAGTTC+CGG + chr4_1:53879921-53879940 Msa0555860:CDS 45.0%
!! CTTACGATTTTGACGCCTCA+CGG - chr4_1:53879998-53880017 None:intergenic 45.0%
! TGAGGCGTCAAAATCGTAAG+AGG + chr4_1:53879998-53880017 Msa0555860:CDS 45.0%
GCGTCAAAATCGTAAGAGGA+AGG + chr4_1:53880002-53880021 Msa0555860:CDS 45.0%
GATAACCTTCGCTGTAACTC+CGG - chr4_1:53880167-53880186 None:intergenic 45.0%
GTTACAGCGAAGGTTATCAC+GGG + chr4_1:53880169-53880188 Msa0555860:CDS 45.0%
GCTAGGTGAAACTGTTGCTA+AGG + chr4_1:53881239-53881258 Msa0555860:three_prime_UTR 45.0%
TCTATGAAGAGATTTGGCGG+TGG - chr4_1:53879807-53879826 None:intergenic 45.0%
GAATGAAAACGGTGGAGAGA+GGG - chr4_1:53879843-53879862 None:intergenic 45.0%
AGAATGAAAACGGTGGAGAG+AGG - chr4_1:53879844-53879863 None:intergenic 45.0%
! CACGTCGGAATCTTTAGTTC+CGG + chr4_1:53879921-53879940 Msa0555860:CDS 45.0%
!! CTTACGATTTTGACGCCTCA+CGG - chr4_1:53879998-53880017 None:intergenic 45.0%
! TGAGGCGTCAAAATCGTAAG+AGG + chr4_1:53879998-53880017 Msa0555860:CDS 45.0%
GCGTCAAAATCGTAAGAGGA+AGG + chr4_1:53880002-53880021 Msa0555860:CDS 45.0%
GATAACCTTCGCTGTAACTC+CGG - chr4_1:53880167-53880186 None:intergenic 45.0%
GTTACAGCGAAGGTTATCAC+GGG + chr4_1:53880169-53880188 Msa0555860:CDS 45.0%
GCTAGGTGAAACTGTTGCTA+AGG + chr4_1:53881239-53881258 Msa0555860:three_prime_UTR 45.0%
TGAAGAGATTTGGCGGTGGA+GGG - chr4_1:53879803-53879822 None:intergenic 50.0%
ATGAAGAGATTTGGCGGTGG+AGG - chr4_1:53879804-53879823 None:intergenic 50.0%
AAAACGGTGGAGAGAGGGAA+AGG - chr4_1:53879838-53879857 None:intergenic 50.0%
GCTCCGAAGAATGAAAACGG+TGG - chr4_1:53879851-53879870 None:intergenic 50.0%
CCGGCTCCGAAGAATGAAAA+CGG - chr4_1:53879854-53879873 None:intergenic 50.0%
! CCGTTTTCATTCTTCGGAGC+CGG + chr4_1:53879851-53879870 Msa0555860:five_prime_UTR 50.0%
! GAGCCGGATGAGGTTTTTCT+TGG + chr4_1:53879867-53879886 Msa0555860:exon 50.0%
! GTCGGAATCTTTAGTTCCGG+CGG + chr4_1:53879924-53879943 Msa0555860:CDS 50.0%
! TGAAGGTAGGTGGCTAGTTC+CGG + chr4_1:53879951-53879970 Msa0555860:CDS 50.0%
AAGAAGAAGAGTGGTGCTCG+TGG + chr4_1:53880120-53880139 Msa0555860:CDS 50.0%
! GTGCTGGTAGGGTTTATCAC+CGG + chr4_1:53880145-53880164 Msa0555860:CDS 50.0%
TATCACCGGAGTTACAGCGA+AGG + chr4_1:53880159-53880178 Msa0555860:CDS 50.0%
AAGTGGAGCTGAGCTATCGT+TGG + chr4_1:53880921-53880940 Msa0555860:intron 50.0%
! AATGGCGTTGGAGAGAATGC+TGG - chr4_1:53881165-53881184 None:intergenic 50.0%
TGAAGAGATTTGGCGGTGGA+GGG - chr4_1:53879803-53879822 None:intergenic 50.0%
ATGAAGAGATTTGGCGGTGG+AGG - chr4_1:53879804-53879823 None:intergenic 50.0%
AAAACGGTGGAGAGAGGGAA+AGG - chr4_1:53879838-53879857 None:intergenic 50.0%
GCTCCGAAGAATGAAAACGG+TGG - chr4_1:53879851-53879870 None:intergenic 50.0%
CCGGCTCCGAAGAATGAAAA+CGG - chr4_1:53879854-53879873 None:intergenic 50.0%
! CCGTTTTCATTCTTCGGAGC+CGG + chr4_1:53879851-53879870 Msa0555860:five_prime_UTR 50.0%
! GAGCCGGATGAGGTTTTTCT+TGG + chr4_1:53879867-53879886 Msa0555860:exon 50.0%
! GTCGGAATCTTTAGTTCCGG+CGG + chr4_1:53879924-53879943 Msa0555860:CDS 50.0%
! TGAAGGTAGGTGGCTAGTTC+CGG + chr4_1:53879951-53879970 Msa0555860:CDS 50.0%
AAGAAGAAGAGTGGTGCTCG+TGG + chr4_1:53880120-53880139 Msa0555860:CDS 50.0%
! GTGCTGGTAGGGTTTATCAC+CGG + chr4_1:53880145-53880164 Msa0555860:CDS 50.0%
TATCACCGGAGTTACAGCGA+AGG + chr4_1:53880159-53880178 Msa0555860:CDS 50.0%
AAGTGGAGCTGAGCTATCGT+TGG + chr4_1:53880921-53880940 Msa0555860:intron 50.0%
! AATGGCGTTGGAGAGAATGC+TGG - chr4_1:53881165-53881184 None:intergenic 50.0%
TCATTCTTCGGAGCCGGATG+AGG + chr4_1:53879857-53879876 Msa0555860:exon 55.0%
TTTCTTGTTGCGCATCGCCG+CGG + chr4_1:53879896-53879915 Msa0555860:CDS 55.0%
AAAGATTCCGACGTGTGCCG+CGG - chr4_1:53879916-53879935 None:intergenic 55.0%
! GGAATCTTTAGTTCCGGCGG+AGG + chr4_1:53879927-53879946 Msa0555860:CDS 55.0%
TTAGTTCCGGCGGAGGATGA+AGG + chr4_1:53879934-53879953 Msa0555860:CDS 55.0%
ACGGAAGCCACAGCAACAAC+GGG - chr4_1:53879979-53879998 None:intergenic 55.0%
CACGGAAGCCACAGCAACAA+CGG - chr4_1:53879980-53879999 None:intergenic 55.0%
TTGTTGCTGTGGCTTCCGTG+AGG + chr4_1:53879980-53879999 Msa0555860:CDS 55.0%
!! TAAGAGGAAGGTTGGTGCGC+CGG + chr4_1:53880014-53880033 Msa0555860:CDS 55.0%
GCCTTCGTTGGGGTCGATAT+CGG + chr4_1:53880044-53880063 Msa0555860:CDS 55.0%
TCCGATATCGACCCCAACGA+AGG - chr4_1:53880048-53880067 None:intergenic 55.0%
TTCGTTGGGGTCGATATCGG+AGG + chr4_1:53880047-53880066 Msa0555860:CDS 55.0%
TATAGCGCCGCAGAAATGCG+CGG + chr4_1:53880074-53880093 Msa0555860:CDS 55.0%
AGGGAGGGGAAGAAGAAGAG+TGG + chr4_1:53880111-53880130 Msa0555860:CDS 55.0%
AAGAAGAGTGGTGCTCGTGG+TGG + chr4_1:53880123-53880142 Msa0555860:CDS 55.0%
TCATTCTTCGGAGCCGGATG+AGG + chr4_1:53879857-53879876 Msa0555860:exon 55.0%
TTTCTTGTTGCGCATCGCCG+CGG + chr4_1:53879896-53879915 Msa0555860:CDS 55.0%
AAAGATTCCGACGTGTGCCG+CGG - chr4_1:53879916-53879935 None:intergenic 55.0%
! GGAATCTTTAGTTCCGGCGG+AGG + chr4_1:53879927-53879946 Msa0555860:CDS 55.0%
TTAGTTCCGGCGGAGGATGA+AGG + chr4_1:53879934-53879953 Msa0555860:CDS 55.0%
ACGGAAGCCACAGCAACAAC+GGG - chr4_1:53879979-53879998 None:intergenic 55.0%
CACGGAAGCCACAGCAACAA+CGG - chr4_1:53879980-53879999 None:intergenic 55.0%
TTGTTGCTGTGGCTTCCGTG+AGG + chr4_1:53879980-53879999 Msa0555860:CDS 55.0%
!! TAAGAGGAAGGTTGGTGCGC+CGG + chr4_1:53880014-53880033 Msa0555860:CDS 55.0%
GCCTTCGTTGGGGTCGATAT+CGG + chr4_1:53880044-53880063 Msa0555860:CDS 55.0%
TCCGATATCGACCCCAACGA+AGG - chr4_1:53880048-53880067 None:intergenic 55.0%
TTCGTTGGGGTCGATATCGG+AGG + chr4_1:53880047-53880066 Msa0555860:CDS 55.0%
TATAGCGCCGCAGAAATGCG+CGG + chr4_1:53880074-53880093 Msa0555860:CDS 55.0%
AGGGAGGGGAAGAAGAAGAG+TGG + chr4_1:53880111-53880130 Msa0555860:CDS 55.0%
AAGAAGAGTGGTGCTCGTGG+TGG + chr4_1:53880123-53880142 Msa0555860:CDS 55.0%
TTCCGGCGGAGGATGAAGGT+AGG + chr4_1:53879938-53879957 Msa0555860:CDS 60.0%
CACCTACCTTCATCCTCCGC+CGG - chr4_1:53879943-53879962 None:intergenic 60.0%
CCCAACGAAGGCCTCCAATC+CGG - chr4_1:53880036-53880055 None:intergenic 60.0%
! CCGGATTGGAGGCCTTCGTT+GGG + chr4_1:53880033-53880052 Msa0555860:CDS 60.0%
! CGGATTGGAGGCCTTCGTTG+GGG + chr4_1:53880034-53880053 Msa0555860:CDS 60.0%
GAAGCCACCGCGCATTTCTG+CGG - chr4_1:53880084-53880103 None:intergenic 60.0%
AAATGCGCGGTGGCTTCTGC+TGG + chr4_1:53880087-53880106 Msa0555860:CDS 60.0%
GTGGCTTCTGCTGGAAGGGA+GGG + chr4_1:53880096-53880115 Msa0555860:CDS 60.0%
TGGCTTCTGCTGGAAGGGAG+GGG + chr4_1:53880097-53880116 Msa0555860:CDS 60.0%
!! TGCTCGTGGTGGTGCTGGTA+GGG + chr4_1:53880134-53880153 Msa0555860:CDS 60.0%
TTCCGGCGGAGGATGAAGGT+AGG + chr4_1:53879938-53879957 Msa0555860:CDS 60.0%
CACCTACCTTCATCCTCCGC+CGG - chr4_1:53879943-53879962 None:intergenic 60.0%
CCCAACGAAGGCCTCCAATC+CGG - chr4_1:53880036-53880055 None:intergenic 60.0%
! CCGGATTGGAGGCCTTCGTT+GGG + chr4_1:53880033-53880052 Msa0555860:CDS 60.0%
! CGGATTGGAGGCCTTCGTTG+GGG + chr4_1:53880034-53880053 Msa0555860:CDS 60.0%
GAAGCCACCGCGCATTTCTG+CGG - chr4_1:53880084-53880103 None:intergenic 60.0%
AAATGCGCGGTGGCTTCTGC+TGG + chr4_1:53880087-53880106 Msa0555860:CDS 60.0%
GTGGCTTCTGCTGGAAGGGA+GGG + chr4_1:53880096-53880115 Msa0555860:CDS 60.0%
TGGCTTCTGCTGGAAGGGAG+GGG + chr4_1:53880097-53880116 Msa0555860:CDS 60.0%
!! TGCTCGTGGTGGTGCTGGTA+GGG + chr4_1:53880134-53880153 Msa0555860:CDS 60.0%
CGGCGGAGGATGAAGGTAGG+TGG + chr4_1:53879941-53879960 Msa0555860:CDS 65.0%
TCCGGCTCCCGTTGTTGCTG+TGG + chr4_1:53879969-53879988 Msa0555860:CDS 65.0%
GCCACAGCAACAACGGGAGC+CGG - chr4_1:53879973-53879992 None:intergenic 65.0%
!! GGAAGGTTGGTGCGCCGGAT+TGG + chr4_1:53880019-53880038 Msa0555860:CDS 65.0%
!! AGGTTGGTGCGCCGGATTGG+AGG + chr4_1:53880022-53880041 Msa0555860:CDS 65.0%
! GCCGGATTGGAGGCCTTCGT+TGG + chr4_1:53880032-53880051 Msa0555860:CDS 65.0%
CGCGGTGGCTTCTGCTGGAA+GGG + chr4_1:53880092-53880111 Msa0555860:CDS 65.0%
GGTGGCTTCTGCTGGAAGGG+AGG + chr4_1:53880095-53880114 Msa0555860:CDS 65.0%
! AGTGGTGCTCGTGGTGGTGC+TGG + chr4_1:53880129-53880148 Msa0555860:CDS 65.0%
!! GTGCTCGTGGTGGTGCTGGT+AGG + chr4_1:53880133-53880152 Msa0555860:CDS 65.0%
CGGCGGAGGATGAAGGTAGG+TGG + chr4_1:53879941-53879960 Msa0555860:CDS 65.0%
TCCGGCTCCCGTTGTTGCTG+TGG + chr4_1:53879969-53879988 Msa0555860:CDS 65.0%
GCCACAGCAACAACGGGAGC+CGG - chr4_1:53879973-53879992 None:intergenic 65.0%
!! GGAAGGTTGGTGCGCCGGAT+TGG + chr4_1:53880019-53880038 Msa0555860:CDS 65.0%
!! AGGTTGGTGCGCCGGATTGG+AGG + chr4_1:53880022-53880041 Msa0555860:CDS 65.0%
! GCCGGATTGGAGGCCTTCGT+TGG + chr4_1:53880032-53880051 Msa0555860:CDS 65.0%
CGCGGTGGCTTCTGCTGGAA+GGG + chr4_1:53880092-53880111 Msa0555860:CDS 65.0%
GGTGGCTTCTGCTGGAAGGG+AGG + chr4_1:53880095-53880114 Msa0555860:CDS 65.0%
! AGTGGTGCTCGTGGTGGTGC+TGG + chr4_1:53880129-53880148 Msa0555860:CDS 65.0%
!! GTGCTCGTGGTGGTGCTGGT+AGG + chr4_1:53880133-53880152 Msa0555860:CDS 65.0%
AGCGCCGCAGAAATGCGCGG+TGG + chr4_1:53880077-53880096 Msa0555860:CDS 70.0%
GCGCGGTGGCTTCTGCTGGA+AGG + chr4_1:53880091-53880110 Msa0555860:CDS 70.0%
AGCGCCGCAGAAATGCGCGG+TGG + chr4_1:53880077-53880096 Msa0555860:CDS 70.0%
GCGCGGTGGCTTCTGCTGGA+AGG + chr4_1:53880091-53880110 Msa0555860:CDS 70.0%
CGCATCGCCGCGGCACACGT+CGG + chr4_1:53879906-53879925 Msa0555860:CDS 75.0%
CGCATCGCCGCGGCACACGT+CGG + chr4_1:53879906-53879925 Msa0555860:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 53879704 53881379 53879704 ID=Msa0555860;Name=Msa0555860
chr4_1 mRNA 53879704 53881379 53879704 ID=Msa0555860-mRNA-1;Parent=Msa0555860;Name=Msa0555860-mRNA-1;_AED=0.00;_eAED=0.00;_QI=170|1|1|1|0|0|2|185|124
chr4_1 exon 53879704 53880190 53879704 ID=Msa0555860-mRNA-1:exon:15689;Parent=Msa0555860-mRNA-1
chr4_1 exon 53881137 53881379 53881137 ID=Msa0555860-mRNA-1:exon:15690;Parent=Msa0555860-mRNA-1
chr4_1 five_prime_UTR 53879704 53879873 53879704 ID=Msa0555860-mRNA-1:five_prime_utr;Parent=Msa0555860-mRNA-1
chr4_1 CDS 53879874 53880190 53879874 ID=Msa0555860-mRNA-1:cds;Parent=Msa0555860-mRNA-1
chr4_1 CDS 53881137 53881194 53881137 ID=Msa0555860-mRNA-1:cds;Parent=Msa0555860-mRNA-1
chr4_1 three_prime_UTR 53881195 53881379 53881195 ID=Msa0555860-mRNA-1:three_prime_utr;Parent=Msa0555860-mRNA-1
Gene Sequence

>Msa0555860

ATGAGGTTTTTCTTGGAATTCGTTTCTTGTTGCGCATCGCCGCGGCACACGTCGGAATCTTTAGTTCCGGCGGAGGATGAAGGTAGGTGGCTAGTTCCGGCTCCCGTTGTTGCTGTGGCTTCCGTGAGGCGTCAAAATCGTAAGAGGAAGGTTGGTGCGCCGGATTGGAGGCCTTCGTTGGGGTCGATATCGGAGGATGTTATAGCGCCGCAGAAATGCGCGGTGGCTTCTGCTGGAAGGGAGGGGAAGAAGAAGAGTGGTGCTCGTGGTGGTGCTGGTAGGGTTTATCACCGGAGTTACAGCGAAGGTTATCACGGGTCATCTTCTATGCATATGATTATGCCAGCATTCTCTCCAACGCCATTCATGTTTTAA
Protein sequence

>Msa0555860

MRFFLEFVSCCASPRHTSESLVPAEDEGRWLVPAPVVAVASVRRQNRKRKVGAPDWRPSLGSISEDVIAPQKCAVASAGREGKKKSGARGGAGRVYHRSYSEGYHGSSSMHMIMPAFSPTPFMF*