AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0622420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0622420 MtrunA17_Chr7g0238631 78.431 51 11 0 2 52 43 93 2.64e-22 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0622420 AT2G13960.1 65.116 43 15 0 10 52 65 107 2.13e-15 65.5
Msa0622420 AT2G13960.2 65.116 43 15 0 10 52 30 72 2.75e-15 64.3
Msa0622420 AT5G02320.1 65.116 43 15 0 10 52 65 107 2.78e-14 65.1
Msa0622420 AT5G02320.2 65.116 43 15 0 10 52 65 107 2.78e-14 65.1
Msa0622420 AT5G02320.3 65.116 43 15 0 10 52 5 47 3.82e-14 64.7
Msa0622420 AT3G09370.2 61.224 49 16 1 7 52 67 115 1.13e-13 63.5
Msa0622420 AT3G09370.1 56.604 53 20 1 7 56 62 114 1.14e-13 63.5
Msa0622420 AT3G09370.3 61.224 49 16 1 7 52 75 123 1.15e-13 63.5

Find 61 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTAATTGGTCAATTATT+TGG 0.166929 4_2:-74851377 None:intergenic
CCACCCTTAGCGTGCCTTAT+AGG 0.202151 4_2:-74852534 None:intergenic
TATTGTAGGATGAGACATTA+AGG 0.293653 4_2:+74852684 Msa0622420:intron
TGGATTTATGTTGGTGGAGG+TGG 0.309935 4_2:-74851524 None:intergenic
GGAAAACATTGGAAGAAAAT+AGG 0.311225 4_2:+74852728 Msa0622420:CDS
TGTTTGGGCTTCTCTCTAAT+TGG 0.328403 4_2:-74851389 None:intergenic
CTCATCTTCAAATCATATCT+AGG 0.331174 4_2:+74851711 Msa0622420:five_prime_UTR
AGTGTGAGGGAGAAGATATC+AGG 0.349806 4_2:-74851550 None:intergenic
TTGTAATCATGTCTCATAAC+TGG 0.356897 4_2:+74853224 Msa0622420:three_prime_UTR
TGCCACTTCAATAGAAAGAT+TGG 0.375619 4_2:-74852886 None:intergenic
GGATATGGATGTTTGTTGTT+TGG 0.389621 4_2:+74852175 Msa0622420:five_prime_UTR
ATTGAAGTGGCAAGGATGAT+TGG 0.406073 4_2:+74852897 Msa0622420:three_prime_UTR
GGAGGAGAACAAGTCCTATA+AGG 0.409948 4_2:+74852520 Msa0622420:intron
TTTGCCTCTGCACTTTGAGC+TGG 0.415507 4_2:+74853174 Msa0622420:three_prime_UTR
GTAATGTTTCATACATTTCA+AGG 0.423379 4_2:-74853073 None:intergenic
AGGGAGAAGATATCAGGGTT+TGG 0.428253 4_2:-74851544 None:intergenic
AGGGTTTGGATTTATGTTGG+TGG 0.431049 4_2:-74851530 None:intergenic
AATTGAAAACCGCATCTTGA+AGG 0.449911 4_2:-74851662 None:intergenic
AGTATAATTCAAAAGTCTCC+AGG 0.458256 4_2:+74852250 Msa0622420:five_prime_UTR
TTTGTCAAATGCATTAGCTC+AGG 0.463353 4_2:-74852761 None:intergenic
ATCAGGGTTTGGATTTATGT+TGG 0.464167 4_2:-74851533 None:intergenic
TTTGTGAATCGGAGAAGAAA+GGG 0.464883 4_2:-74851635 None:intergenic
GTCCAATCTTTCTATTGAAG+TGG 0.468597 4_2:+74852884 Msa0622420:three_prime_UTR
AGAAAGGGAGGAGGTTCAGA+TGG 0.474386 4_2:-74851620 None:intergenic
AGGCGGTGTGGATGTAGCCA+TGG 0.485223 4_2:-74852284 None:intergenic
GTTTGGATTTATGTTGGTGG+AGG 0.500329 4_2:-74851527 None:intergenic
GGCGGTGTGGATGTAGCCAT+GGG 0.512625 4_2:-74852283 None:intergenic
GAAGGAGAGAAATGTTACAT+AGG 0.517797 4_2:-74851465 None:intergenic
GAGGGAAGGAAGATAAAGAA+GGG 0.520886 4_2:-74851579 None:intergenic
GAAATGTTACATAGGAAATG+TGG 0.521689 4_2:-74851457 None:intergenic
GTGTGAGGGAGAAGATATCA+GGG 0.524168 4_2:-74851549 None:intergenic
TGAGGGAAGGAAGATAAAGA+AGG 0.525432 4_2:-74851580 None:intergenic
AAGTCCTATAAGGCACGCTA+AGG 0.528953 4_2:+74852530 Msa0622420:CDS
TCATCTTCAAATCATATCTA+GGG 0.535900 4_2:+74851712 Msa0622420:five_prime_UTR
GATTATGAAGTAAAGACAAA+AGG 0.537647 4_2:-74852924 None:intergenic
ATCTTTCTATTGAAGTGGCA+AGG 0.545324 4_2:+74852889 Msa0622420:three_prime_UTR
GCCATGGGGCTACATATCCC+TGG 0.552664 4_2:-74852268 None:intergenic
TCTTGCTCATCTGTGTCTGA+AGG 0.559484 4_2:+74852220 Msa0622420:five_prime_UTR
ACAAAATCTCCTTCAAGATG+CGG 0.561408 4_2:+74851653 Msa0622420:five_prime_UTR
GTGAATCGGAGAAGAAAGGG+AGG 0.561423 4_2:-74851632 None:intergenic
TAAGGCACGCTAAGGGTGGT+TGG 0.562887 4_2:+74852538 Msa0622420:CDS
AAAGAGAAGCATGTGAGGGA+AGG 0.591322 4_2:-74851593 None:intergenic
AACAAACATTACCGATGTGA+AGG 0.591884 4_2:-74852304 None:intergenic
AGAAGGGGAAAATAGTGTGA+GGG 0.595581 4_2:-74851563 None:intergenic
GTGGCAAAGAGAAGCATGTG+AGG 0.601561 4_2:-74851598 None:intergenic
GGGTGGTTGGACAGCAGAAG+AGG 0.605706 4_2:+74852551 Msa0622420:CDS
AGGGAAGGAAGATAAAGAAG+GGG 0.607295 4_2:-74851578 None:intergenic
CCTATAAGGCACGCTAAGGG+TGG 0.618742 4_2:+74852534 Msa0622420:CDS
AGTCCTATAAGGCACGCTAA+GGG 0.620051 4_2:+74852531 Msa0622420:CDS
AATCGGAGAAGAAAGGGAGG+AGG 0.620302 4_2:-74851629 None:intergenic
AAGAAGGGGAAAATAGTGTG+AGG 0.621252 4_2:-74851564 None:intergenic
AAGGGAGGAGGTTCAGATGG+TGG 0.627661 4_2:-74851617 None:intergenic
ACAAACAAAATCAATAGCAG+AGG 0.629915 4_2:-74853205 None:intergenic
GTATAATTCAAAAGTCTCCA+GGG 0.641290 4_2:+74852251 Msa0622420:five_prime_UTR
TCCAGGGATATGTAGCCCCA+TGG 0.641883 4_2:+74852267 Msa0622420:exon
ATGTCCAGCTCAAAGTGCAG+AGG 0.663657 4_2:-74853178 None:intergenic
CATCCACACCGCCTTCACAT+CGG 0.670345 4_2:+74852293 Msa0622420:CDS
TGGCAAAGAGAAGCATGTGA+GGG 0.676512 4_2:-74851597 None:intergenic
AAACATTACCGATGTGAAGG+CGG 0.687678 4_2:-74852301 None:intergenic
TTACCGATGTGAAGGCGGTG+TGG 0.693836 4_2:-74852296 None:intergenic
GCGGTGTGGATGTAGCCATG+GGG 0.784527 4_2:-74852282 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTAGTAATTAAAGA+TGG - chr4_2:74851802-74851821 None:intergenic 10.0%
!! AAATAATTAGTAATTAAAGA+TGG - chr4_2:74851802-74851821 None:intergenic 10.0%
!! AATATAAATGCATTGAATAA+AGG - chr4_2:74851752-74851771 None:intergenic 15.0%
!!! TTTTTTTATTGGTTTTGTTT+TGG + chr4_2:74851962-74851981 Msa0622420:intron 15.0%
!!! GATAAAGATTTTGTTTTTTT+GGG + chr4_2:74851984-74852003 Msa0622420:intron 15.0%
!!! GATTTTTTTTTTTTATACTG+AGG + chr4_2:74852087-74852106 Msa0622420:intron 15.0%
!! AATATAAATGCATTGAATAA+AGG - chr4_2:74851752-74851771 None:intergenic 15.0%
!!! TTTTTTTATTGGTTTTGTTT+TGG + chr4_2:74851962-74851981 Msa0622420:intron 15.0%
!!! GATAAAGATTTTGTTTTTTT+GGG + chr4_2:74851984-74852003 Msa0622420:intron 15.0%
!!! GATTTTTTTTTTTTATACTG+AGG + chr4_2:74852087-74852106 Msa0622420:intron 15.0%
!!! GTCAAAAAACGTTTTTTTAT+TGG + chr4_2:74851951-74851970 Msa0622420:intron 20.0%
!!! GGATAAAGATTTTGTTTTTT+TGG + chr4_2:74851983-74852002 Msa0622420:intron 20.0%
!!! TTTTTTTGGGAAAAAATTGA+AGG + chr4_2:74851997-74852016 Msa0622420:intron 20.0%
!!! TTTTTTTTTTATACTGAGGA+TGG + chr4_2:74852091-74852110 Msa0622420:intron 20.0%
!!! TTTTTAGGGTTTTTTATGTT+TGG + chr4_2:74852114-74852133 Msa0622420:intron 20.0%
!!! TGTTATGTTATTTACGTTTT+TGG + chr4_2:74852323-74852342 Msa0622420:intron 20.0%
!!! CATTTTAATTAGAAAGATTG+AGG + chr4_2:74852961-74852980 Msa0622420:three_prime_UTR 20.0%
!!! GTCAAAAAACGTTTTTTTAT+TGG + chr4_2:74851951-74851970 Msa0622420:intron 20.0%
!!! GGATAAAGATTTTGTTTTTT+TGG + chr4_2:74851983-74852002 Msa0622420:intron 20.0%
!!! TTTTTTTGGGAAAAAATTGA+AGG + chr4_2:74851997-74852016 Msa0622420:intron 20.0%
!!! TTTTTTTTTTATACTGAGGA+TGG + chr4_2:74852091-74852110 Msa0622420:intron 20.0%
!!! TTTTTAGGGTTTTTTATGTT+TGG + chr4_2:74852114-74852133 Msa0622420:intron 20.0%
!!! TGTTATGTTATTTACGTTTT+TGG + chr4_2:74852323-74852342 Msa0622420:intron 20.0%
!!! CATTTTAATTAGAAAGATTG+AGG + chr4_2:74852961-74852980 Msa0622420:three_prime_UTR 20.0%
! TCTCTAATTGGTCAATTATT+TGG - chr4_2:74851380-74851399 None:intergenic 25.0%
! TCATCTTCAAATCATATCTA+GGG + chr4_2:74851712-74851731 Msa0622420:five_prime_UTR 25.0%
!! TTGATTGAGTTTTTGTGTTT+TGG + chr4_2:74851905-74851924 Msa0622420:intron 25.0%
!!! AGTTTTTGTGTTTTGGATAA+AGG + chr4_2:74851912-74851931 Msa0622420:intron 25.0%
!!! GTTTTTGTGTTTTGGATAAA+GGG + chr4_2:74851913-74851932 Msa0622420:intron 25.0%
!!! TTTTTGTGTTTTGGATAAAG+GGG + chr4_2:74851914-74851933 Msa0622420:intron 25.0%
!!! TGAAGGTTTTGATTTGATTA+TGG + chr4_2:74852014-74852033 Msa0622420:intron 25.0%
!!! TTTTTATGTTTGGTGATTGA+TGG + chr4_2:74852124-74852143 Msa0622420:intron 25.0%
!!! TTTTATGTTTGGTGATTGAT+GGG + chr4_2:74852125-74852144 Msa0622420:intron 25.0%
! ATTGAAGAACACAATAAGAA+CGG - chr4_2:74852663-74852682 None:intergenic 25.0%
! TGTGTTCTTCAATTTATTGT+AGG + chr4_2:74852670-74852689 Msa0622420:intron 25.0%
! GATTATGAAGTAAAGACAAA+AGG - chr4_2:74852927-74852946 None:intergenic 25.0%
! GTAATGTTTCATACATTTCA+AGG - chr4_2:74853076-74853095 None:intergenic 25.0%
!!! GTGTTTTTTGTTGATTTTGT+TGG + chr4_2:74853143-74853162 Msa0622420:three_prime_UTR 25.0%
! TCTCTAATTGGTCAATTATT+TGG - chr4_2:74851380-74851399 None:intergenic 25.0%
! TCATCTTCAAATCATATCTA+GGG + chr4_2:74851712-74851731 Msa0622420:five_prime_UTR 25.0%
!! TTGATTGAGTTTTTGTGTTT+TGG + chr4_2:74851905-74851924 Msa0622420:intron 25.0%
!!! AGTTTTTGTGTTTTGGATAA+AGG + chr4_2:74851912-74851931 Msa0622420:intron 25.0%
!!! GTTTTTGTGTTTTGGATAAA+GGG + chr4_2:74851913-74851932 Msa0622420:intron 25.0%
!!! TTTTTGTGTTTTGGATAAAG+GGG + chr4_2:74851914-74851933 Msa0622420:intron 25.0%
!!! TGAAGGTTTTGATTTGATTA+TGG + chr4_2:74852014-74852033 Msa0622420:intron 25.0%
!!! TTTTTATGTTTGGTGATTGA+TGG + chr4_2:74852124-74852143 Msa0622420:intron 25.0%
!!! TTTTATGTTTGGTGATTGAT+GGG + chr4_2:74852125-74852144 Msa0622420:intron 25.0%
! ATTGAAGAACACAATAAGAA+CGG - chr4_2:74852663-74852682 None:intergenic 25.0%
! TGTGTTCTTCAATTTATTGT+AGG + chr4_2:74852670-74852689 Msa0622420:intron 25.0%
! GATTATGAAGTAAAGACAAA+AGG - chr4_2:74852927-74852946 None:intergenic 25.0%
! GTAATGTTTCATACATTTCA+AGG - chr4_2:74853076-74853095 None:intergenic 25.0%
!!! GTGTTTTTTGTTGATTTTGT+TGG + chr4_2:74853143-74853162 Msa0622420:three_prime_UTR 25.0%
! TTTTGCTTGTAGTCATGTTT+GGG - chr4_2:74851407-74851426 None:intergenic 30.0%
! TTTTTGCTTGTAGTCATGTT+TGG - chr4_2:74851408-74851427 None:intergenic 30.0%
GAAATGTTACATAGGAAATG+TGG - chr4_2:74851460-74851479 None:intergenic 30.0%
!! TTTTGAAAAGAGAGAAAGGA+AGG - chr4_2:74851486-74851505 None:intergenic 30.0%
! CCTTTCTCTCTTTTCAAAAT+CGG + chr4_2:74851487-74851506 Msa0622420:five_prime_UTR 30.0%
! TTGAAGGAGATTTTGTGAAT+CGG - chr4_2:74851649-74851668 None:intergenic 30.0%
CTCATCTTCAAATCATATCT+AGG + chr4_2:74851711-74851730 Msa0622420:five_prime_UTR 30.0%
TCGAAGAATTTCAAGATTTC+TGG + chr4_2:74851865-74851884 Msa0622420:intron 30.0%
!!! TAAAGGGGTTTGATTTGTTT+TGG + chr4_2:74851929-74851948 Msa0622420:intron 30.0%
!!! TGATTATGGATTTTGAGATC+TGG + chr4_2:74852028-74852047 Msa0622420:intron 30.0%
!!! GATTATGGATTTTGAGATCT+GGG + chr4_2:74852029-74852048 Msa0622420:intron 30.0%
! TGAGTAAAAGTGTGAAAGTT+TGG + chr4_2:74852065-74852084 Msa0622420:intron 30.0%
! TTATACTGAGGATGGTTTTT+AGG + chr4_2:74852099-74852118 Msa0622420:intron 30.0%
! TATACTGAGGATGGTTTTTA+GGG + chr4_2:74852100-74852119 Msa0622420:intron 30.0%
!! TTTTTAGGGATTGAAGGTTT+TGG + chr4_2:74852148-74852167 Msa0622420:intron 30.0%
AGTATAATTCAAAAGTCTCC+AGG + chr4_2:74852250-74852269 Msa0622420:five_prime_UTR 30.0%
GTATAATTCAAAAGTCTCCA+GGG + chr4_2:74852251-74852270 Msa0622420:five_prime_UTR 30.0%
TTTGGTTGCAGTTGTAATTA+TGG + chr4_2:74852341-74852360 Msa0622420:intron 30.0%
! TTTTATGCCAAATCTGCTAT+TGG + chr4_2:74852400-74852419 Msa0622420:intron 30.0%
AATTACACCAATAGCAGATT+TGG - chr4_2:74852410-74852429 None:intergenic 30.0%
ACCATATGATATGTTTGTAC+AGG + chr4_2:74852499-74852518 Msa0622420:intron 30.0%
TCCTGTACAAACATATCATA+TGG - chr4_2:74852503-74852522 None:intergenic 30.0%
ATATGATATGTTTGTACAGG+AGG + chr4_2:74852502-74852521 Msa0622420:intron 30.0%
AGAGGTTCATATTTGCTTAT+TGG + chr4_2:74852569-74852588 Msa0622420:intron 30.0%
TATTGTAGGATGAGACATTA+AGG + chr4_2:74852684-74852703 Msa0622420:intron 30.0%
! TTTTCCCTTAAAAGCTGTAA+CGG - chr4_2:74852714-74852733 None:intergenic 30.0%
GGAAAACATTGGAAGAAAAT+AGG + chr4_2:74852728-74852747 Msa0622420:CDS 30.0%
!!! TTGTTGATTTTGTTGGCTAT+TGG + chr4_2:74853150-74853169 Msa0622420:three_prime_UTR 30.0%
ACAAACAAAATCAATAGCAG+AGG - chr4_2:74853208-74853227 None:intergenic 30.0%
TTGTAATCATGTCTCATAAC+TGG + chr4_2:74853224-74853243 Msa0622420:three_prime_UTR 30.0%
! TTTTGCTTGTAGTCATGTTT+GGG - chr4_2:74851407-74851426 None:intergenic 30.0%
! TTTTTGCTTGTAGTCATGTT+TGG - chr4_2:74851408-74851427 None:intergenic 30.0%
GAAATGTTACATAGGAAATG+TGG - chr4_2:74851460-74851479 None:intergenic 30.0%
!! TTTTGAAAAGAGAGAAAGGA+AGG - chr4_2:74851486-74851505 None:intergenic 30.0%
! CCTTTCTCTCTTTTCAAAAT+CGG + chr4_2:74851487-74851506 Msa0622420:five_prime_UTR 30.0%
! TTGAAGGAGATTTTGTGAAT+CGG - chr4_2:74851649-74851668 None:intergenic 30.0%
CTCATCTTCAAATCATATCT+AGG + chr4_2:74851711-74851730 Msa0622420:five_prime_UTR 30.0%
TCGAAGAATTTCAAGATTTC+TGG + chr4_2:74851865-74851884 Msa0622420:intron 30.0%
!!! TAAAGGGGTTTGATTTGTTT+TGG + chr4_2:74851929-74851948 Msa0622420:intron 30.0%
!!! TGATTATGGATTTTGAGATC+TGG + chr4_2:74852028-74852047 Msa0622420:intron 30.0%
!!! GATTATGGATTTTGAGATCT+GGG + chr4_2:74852029-74852048 Msa0622420:intron 30.0%
! TGAGTAAAAGTGTGAAAGTT+TGG + chr4_2:74852065-74852084 Msa0622420:intron 30.0%
! TTATACTGAGGATGGTTTTT+AGG + chr4_2:74852099-74852118 Msa0622420:intron 30.0%
! TATACTGAGGATGGTTTTTA+GGG + chr4_2:74852100-74852119 Msa0622420:intron 30.0%
!! TTTTTAGGGATTGAAGGTTT+TGG + chr4_2:74852148-74852167 Msa0622420:intron 30.0%
AGTATAATTCAAAAGTCTCC+AGG + chr4_2:74852250-74852269 Msa0622420:five_prime_UTR 30.0%
GTATAATTCAAAAGTCTCCA+GGG + chr4_2:74852251-74852270 Msa0622420:five_prime_UTR 30.0%
TTTGGTTGCAGTTGTAATTA+TGG + chr4_2:74852341-74852360 Msa0622420:intron 30.0%
! TTTTATGCCAAATCTGCTAT+TGG + chr4_2:74852400-74852419 Msa0622420:intron 30.0%
AATTACACCAATAGCAGATT+TGG - chr4_2:74852410-74852429 None:intergenic 30.0%
ACCATATGATATGTTTGTAC+AGG + chr4_2:74852499-74852518 Msa0622420:intron 30.0%
TCCTGTACAAACATATCATA+TGG - chr4_2:74852503-74852522 None:intergenic 30.0%
ATATGATATGTTTGTACAGG+AGG + chr4_2:74852502-74852521 Msa0622420:intron 30.0%
AGAGGTTCATATTTGCTTAT+TGG + chr4_2:74852569-74852588 Msa0622420:intron 30.0%
TATTGTAGGATGAGACATTA+AGG + chr4_2:74852684-74852703 Msa0622420:intron 30.0%
! TTTTCCCTTAAAAGCTGTAA+CGG - chr4_2:74852714-74852733 None:intergenic 30.0%
GGAAAACATTGGAAGAAAAT+AGG + chr4_2:74852728-74852747 Msa0622420:CDS 30.0%
!!! TTGTTGATTTTGTTGGCTAT+TGG + chr4_2:74853150-74853169 Msa0622420:three_prime_UTR 30.0%
ACAAACAAAATCAATAGCAG+AGG - chr4_2:74853208-74853227 None:intergenic 30.0%
TTGTAATCATGTCTCATAAC+TGG + chr4_2:74853224-74853243 Msa0622420:three_prime_UTR 30.0%
GAAGGAGAGAAATGTTACAT+AGG - chr4_2:74851468-74851487 None:intergenic 35.0%
!! CCGATTTTGAAAAGAGAGAA+AGG - chr4_2:74851490-74851509 None:intergenic 35.0%
ATCAGGGTTTGGATTTATGT+TGG - chr4_2:74851536-74851555 None:intergenic 35.0%
TTTGTGAATCGGAGAAGAAA+GGG - chr4_2:74851638-74851657 None:intergenic 35.0%
! TTTTGTGAATCGGAGAAGAA+AGG - chr4_2:74851639-74851658 None:intergenic 35.0%
ACAAAATCTCCTTCAAGATG+CGG + chr4_2:74851653-74851672 Msa0622420:five_prime_UTR 35.0%
AATTGAAAACCGCATCTTGA+AGG - chr4_2:74851665-74851684 None:intergenic 35.0%
! TCAAGATGCGGTTTTCAATT+CGG + chr4_2:74851665-74851684 Msa0622420:five_prime_UTR 35.0%
! TGAAATTCTTCGAAGAGTGT+TGG - chr4_2:74851858-74851877 None:intergenic 35.0%
!!! TTGGTGATTGATGGGTTTTT+AGG + chr4_2:74852133-74852152 Msa0622420:intron 35.0%
!!! TGGTGATTGATGGGTTTTTA+GGG + chr4_2:74852134-74852153 Msa0622420:intron 35.0%
!! GGATATGGATGTTTGTTGTT+TGG + chr4_2:74852175-74852194 Msa0622420:five_prime_UTR 35.0%
AACAAACATTACCGATGTGA+AGG - chr4_2:74852307-74852326 None:intergenic 35.0%
!! AATAGTTGCTGATTCACTTC+TGG + chr4_2:74852441-74852460 Msa0622420:intron 35.0%
!! ATTCAATCCACTTTTCTGGA+AGG - chr4_2:74852615-74852634 None:intergenic 35.0%
!! TCCAGAAAAGTGGATTGAAT+TGG + chr4_2:74852615-74852634 Msa0622420:intron 35.0%
!! CCAGAAAAGTGGATTGAATT+GGG + chr4_2:74852616-74852635 Msa0622420:intron 35.0%
! AATGCCGTTACAGCTTTTAA+GGG + chr4_2:74852707-74852726 Msa0622420:CDS 35.0%
!! CAGCTTTTAAGGGAAAACAT+TGG + chr4_2:74852717-74852736 Msa0622420:CDS 35.0%
TTTGTCAAATGCATTAGCTC+AGG - chr4_2:74852764-74852783 None:intergenic 35.0%
GTCCAATCTTTCTATTGAAG+TGG + chr4_2:74852884-74852903 Msa0622420:three_prime_UTR 35.0%
! TGCCACTTCAATAGAAAGAT+TGG - chr4_2:74852889-74852908 None:intergenic 35.0%
ATCTTTCTATTGAAGTGGCA+AGG + chr4_2:74852889-74852908 Msa0622420:three_prime_UTR 35.0%
GAAGGAGAGAAATGTTACAT+AGG - chr4_2:74851468-74851487 None:intergenic 35.0%
!! CCGATTTTGAAAAGAGAGAA+AGG - chr4_2:74851490-74851509 None:intergenic 35.0%
ATCAGGGTTTGGATTTATGT+TGG - chr4_2:74851536-74851555 None:intergenic 35.0%
TTTGTGAATCGGAGAAGAAA+GGG - chr4_2:74851638-74851657 None:intergenic 35.0%
! TTTTGTGAATCGGAGAAGAA+AGG - chr4_2:74851639-74851658 None:intergenic 35.0%
ACAAAATCTCCTTCAAGATG+CGG + chr4_2:74851653-74851672 Msa0622420:five_prime_UTR 35.0%
AATTGAAAACCGCATCTTGA+AGG - chr4_2:74851665-74851684 None:intergenic 35.0%
! TCAAGATGCGGTTTTCAATT+CGG + chr4_2:74851665-74851684 Msa0622420:five_prime_UTR 35.0%
! TGAAATTCTTCGAAGAGTGT+TGG - chr4_2:74851858-74851877 None:intergenic 35.0%
!!! TTGGTGATTGATGGGTTTTT+AGG + chr4_2:74852133-74852152 Msa0622420:intron 35.0%
!!! TGGTGATTGATGGGTTTTTA+GGG + chr4_2:74852134-74852153 Msa0622420:intron 35.0%
!! GGATATGGATGTTTGTTGTT+TGG + chr4_2:74852175-74852194 Msa0622420:five_prime_UTR 35.0%
AACAAACATTACCGATGTGA+AGG - chr4_2:74852307-74852326 None:intergenic 35.0%
!! AATAGTTGCTGATTCACTTC+TGG + chr4_2:74852441-74852460 Msa0622420:intron 35.0%
!! ATTCAATCCACTTTTCTGGA+AGG - chr4_2:74852615-74852634 None:intergenic 35.0%
!! TCCAGAAAAGTGGATTGAAT+TGG + chr4_2:74852615-74852634 Msa0622420:intron 35.0%
!! CCAGAAAAGTGGATTGAATT+GGG + chr4_2:74852616-74852635 Msa0622420:intron 35.0%
! AATGCCGTTACAGCTTTTAA+GGG + chr4_2:74852707-74852726 Msa0622420:CDS 35.0%
!! CAGCTTTTAAGGGAAAACAT+TGG + chr4_2:74852717-74852736 Msa0622420:CDS 35.0%
TTTGTCAAATGCATTAGCTC+AGG - chr4_2:74852764-74852783 None:intergenic 35.0%
GTCCAATCTTTCTATTGAAG+TGG + chr4_2:74852884-74852903 Msa0622420:three_prime_UTR 35.0%
! TGCCACTTCAATAGAAAGAT+TGG - chr4_2:74852889-74852908 None:intergenic 35.0%
ATCTTTCTATTGAAGTGGCA+AGG + chr4_2:74852889-74852908 Msa0622420:three_prime_UTR 35.0%
TGTTTGGGCTTCTCTCTAAT+TGG - chr4_2:74851392-74851411 None:intergenic 40.0%
! GTTTGGATTTATGTTGGTGG+AGG - chr4_2:74851530-74851549 None:intergenic 40.0%
AGGGTTTGGATTTATGTTGG+TGG - chr4_2:74851533-74851552 None:intergenic 40.0%
AGAAGGGGAAAATAGTGTGA+GGG - chr4_2:74851566-74851585 None:intergenic 40.0%
AAGAAGGGGAAAATAGTGTG+AGG - chr4_2:74851567-74851586 None:intergenic 40.0%
AGGGAAGGAAGATAAAGAAG+GGG - chr4_2:74851581-74851600 None:intergenic 40.0%
GAGGGAAGGAAGATAAAGAA+GGG - chr4_2:74851582-74851601 None:intergenic 40.0%
TGAGGGAAGGAAGATAAAGA+AGG - chr4_2:74851583-74851602 None:intergenic 40.0%
!! GATGGGTTTTTAGGGATTGA+AGG + chr4_2:74852142-74852161 Msa0622420:intron 40.0%
!! GAAGGTTTTGGTGAAGGATA+TGG + chr4_2:74852160-74852179 Msa0622420:five_prime_UTR 40.0%
AAACATTACCGATGTGAAGG+CGG - chr4_2:74852304-74852323 None:intergenic 40.0%
! TTTTCCTCCTTCCAGAAAAG+TGG + chr4_2:74852605-74852624 Msa0622420:intron 40.0%
!! CCCAATTCAATCCACTTTTC+TGG - chr4_2:74852619-74852638 None:intergenic 40.0%
! GAATGCCGTTACAGCTTTTA+AGG + chr4_2:74852706-74852725 Msa0622420:CDS 40.0%
ATTGAAGTGGCAAGGATGAT+TGG + chr4_2:74852897-74852916 Msa0622420:three_prime_UTR 40.0%
TGTTTGGGCTTCTCTCTAAT+TGG - chr4_2:74851392-74851411 None:intergenic 40.0%
! GTTTGGATTTATGTTGGTGG+AGG - chr4_2:74851530-74851549 None:intergenic 40.0%
AGGGTTTGGATTTATGTTGG+TGG - chr4_2:74851533-74851552 None:intergenic 40.0%
AGAAGGGGAAAATAGTGTGA+GGG - chr4_2:74851566-74851585 None:intergenic 40.0%
AAGAAGGGGAAAATAGTGTG+AGG - chr4_2:74851567-74851586 None:intergenic 40.0%
AGGGAAGGAAGATAAAGAAG+GGG - chr4_2:74851581-74851600 None:intergenic 40.0%
GAGGGAAGGAAGATAAAGAA+GGG - chr4_2:74851582-74851601 None:intergenic 40.0%
TGAGGGAAGGAAGATAAAGA+AGG - chr4_2:74851583-74851602 None:intergenic 40.0%
!! GATGGGTTTTTAGGGATTGA+AGG + chr4_2:74852142-74852161 Msa0622420:intron 40.0%
!! GAAGGTTTTGGTGAAGGATA+TGG + chr4_2:74852160-74852179 Msa0622420:five_prime_UTR 40.0%
AAACATTACCGATGTGAAGG+CGG - chr4_2:74852304-74852323 None:intergenic 40.0%
! TTTTCCTCCTTCCAGAAAAG+TGG + chr4_2:74852605-74852624 Msa0622420:intron 40.0%
!! CCCAATTCAATCCACTTTTC+TGG - chr4_2:74852619-74852638 None:intergenic 40.0%
! GAATGCCGTTACAGCTTTTA+AGG + chr4_2:74852706-74852725 Msa0622420:CDS 40.0%
ATTGAAGTGGCAAGGATGAT+TGG + chr4_2:74852897-74852916 Msa0622420:three_prime_UTR 40.0%
! TGGATTTATGTTGGTGGAGG+TGG - chr4_2:74851527-74851546 None:intergenic 45.0%
AGGGAGAAGATATCAGGGTT+TGG - chr4_2:74851547-74851566 None:intergenic 45.0%
GTGTGAGGGAGAAGATATCA+GGG - chr4_2:74851552-74851571 None:intergenic 45.0%
AGTGTGAGGGAGAAGATATC+AGG - chr4_2:74851553-74851572 None:intergenic 45.0%
! AAAGAGAAGCATGTGAGGGA+AGG - chr4_2:74851596-74851615 None:intergenic 45.0%
! TGGCAAAGAGAAGCATGTGA+GGG - chr4_2:74851600-74851619 None:intergenic 45.0%
!! GGGATTGAAGGTTTTGGTGA+AGG + chr4_2:74852154-74852173 Msa0622420:intron 45.0%
TCTTGCTCATCTGTGTCTGA+AGG + chr4_2:74852220-74852239 Msa0622420:five_prime_UTR 45.0%
GGAGGAGAACAAGTCCTATA+AGG + chr4_2:74852520-74852539 Msa0622420:intron 45.0%
AAGTCCTATAAGGCACGCTA+AGG + chr4_2:74852530-74852549 Msa0622420:CDS 45.0%
AGTCCTATAAGGCACGCTAA+GGG + chr4_2:74852531-74852550 Msa0622420:CDS 45.0%
!! CAATCCACTTTTCTGGAAGG+AGG - chr4_2:74852612-74852631 None:intergenic 45.0%
! TGGATTTATGTTGGTGGAGG+TGG - chr4_2:74851527-74851546 None:intergenic 45.0%
AGGGAGAAGATATCAGGGTT+TGG - chr4_2:74851547-74851566 None:intergenic 45.0%
GTGTGAGGGAGAAGATATCA+GGG - chr4_2:74851552-74851571 None:intergenic 45.0%
AGTGTGAGGGAGAAGATATC+AGG - chr4_2:74851553-74851572 None:intergenic 45.0%
! AAAGAGAAGCATGTGAGGGA+AGG - chr4_2:74851596-74851615 None:intergenic 45.0%
! TGGCAAAGAGAAGCATGTGA+GGG - chr4_2:74851600-74851619 None:intergenic 45.0%
!! GGGATTGAAGGTTTTGGTGA+AGG + chr4_2:74852154-74852173 Msa0622420:intron 45.0%
TCTTGCTCATCTGTGTCTGA+AGG + chr4_2:74852220-74852239 Msa0622420:five_prime_UTR 45.0%
GGAGGAGAACAAGTCCTATA+AGG + chr4_2:74852520-74852539 Msa0622420:intron 45.0%
AAGTCCTATAAGGCACGCTA+AGG + chr4_2:74852530-74852549 Msa0622420:CDS 45.0%
AGTCCTATAAGGCACGCTAA+GGG + chr4_2:74852531-74852550 Msa0622420:CDS 45.0%
!! CAATCCACTTTTCTGGAAGG+AGG - chr4_2:74852612-74852631 None:intergenic 45.0%
! GTGGCAAAGAGAAGCATGTG+AGG - chr4_2:74851601-74851620 None:intergenic 50.0%
AGAAAGGGAGGAGGTTCAGA+TGG - chr4_2:74851623-74851642 None:intergenic 50.0%
AATCGGAGAAGAAAGGGAGG+AGG - chr4_2:74851632-74851651 None:intergenic 50.0%
GTGAATCGGAGAAGAAAGGG+AGG - chr4_2:74851635-74851654 None:intergenic 50.0%
TTTGCCTCTGCACTTTGAGC+TGG + chr4_2:74853174-74853193 Msa0622420:three_prime_UTR 50.0%
ATGTCCAGCTCAAAGTGCAG+AGG - chr4_2:74853181-74853200 None:intergenic 50.0%
! GTGGCAAAGAGAAGCATGTG+AGG - chr4_2:74851601-74851620 None:intergenic 50.0%
AGAAAGGGAGGAGGTTCAGA+TGG - chr4_2:74851623-74851642 None:intergenic 50.0%
AATCGGAGAAGAAAGGGAGG+AGG - chr4_2:74851632-74851651 None:intergenic 50.0%
GTGAATCGGAGAAGAAAGGG+AGG - chr4_2:74851635-74851654 None:intergenic 50.0%
TTTGCCTCTGCACTTTGAGC+TGG + chr4_2:74853174-74853193 Msa0622420:three_prime_UTR 50.0%
ATGTCCAGCTCAAAGTGCAG+AGG - chr4_2:74853181-74853200 None:intergenic 50.0%
AAGGGAGGAGGTTCAGATGG+TGG - chr4_2:74851620-74851639 None:intergenic 55.0%
TCCAGGGATATGTAGCCCCA+TGG + chr4_2:74852267-74852286 Msa0622420:exon 55.0%
CATCCACACCGCCTTCACAT+CGG + chr4_2:74852293-74852312 Msa0622420:CDS 55.0%
TTACCGATGTGAAGGCGGTG+TGG - chr4_2:74852299-74852318 None:intergenic 55.0%
CCACCCTTAGCGTGCCTTAT+AGG - chr4_2:74852537-74852556 None:intergenic 55.0%
CCTATAAGGCACGCTAAGGG+TGG + chr4_2:74852534-74852553 Msa0622420:CDS 55.0%
TAAGGCACGCTAAGGGTGGT+TGG + chr4_2:74852538-74852557 Msa0622420:CDS 55.0%
AAGGGAGGAGGTTCAGATGG+TGG - chr4_2:74851620-74851639 None:intergenic 55.0%
TCCAGGGATATGTAGCCCCA+TGG + chr4_2:74852267-74852286 Msa0622420:exon 55.0%
CATCCACACCGCCTTCACAT+CGG + chr4_2:74852293-74852312 Msa0622420:CDS 55.0%
TTACCGATGTGAAGGCGGTG+TGG - chr4_2:74852299-74852318 None:intergenic 55.0%
CCACCCTTAGCGTGCCTTAT+AGG - chr4_2:74852537-74852556 None:intergenic 55.0%
CCTATAAGGCACGCTAAGGG+TGG + chr4_2:74852534-74852553 Msa0622420:CDS 55.0%
TAAGGCACGCTAAGGGTGGT+TGG + chr4_2:74852538-74852557 Msa0622420:CDS 55.0%
GCCATGGGGCTACATATCCC+TGG - chr4_2:74852271-74852290 None:intergenic 60.0%
GCGGTGTGGATGTAGCCATG+GGG - chr4_2:74852285-74852304 None:intergenic 60.0%
GGCGGTGTGGATGTAGCCAT+GGG - chr4_2:74852286-74852305 None:intergenic 60.0%
AGGCGGTGTGGATGTAGCCA+TGG - chr4_2:74852287-74852306 None:intergenic 60.0%
GGGTGGTTGGACAGCAGAAG+AGG + chr4_2:74852551-74852570 Msa0622420:CDS 60.0%
GCCATGGGGCTACATATCCC+TGG - chr4_2:74852271-74852290 None:intergenic 60.0%
GCGGTGTGGATGTAGCCATG+GGG - chr4_2:74852285-74852304 None:intergenic 60.0%
GGCGGTGTGGATGTAGCCAT+GGG - chr4_2:74852286-74852305 None:intergenic 60.0%
AGGCGGTGTGGATGTAGCCA+TGG - chr4_2:74852287-74852306 None:intergenic 60.0%
GGGTGGTTGGACAGCAGAAG+AGG + chr4_2:74852551-74852570 Msa0622420:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 74851369 74853296 74851369 ID=Msa0622420;Name=Msa0622420
chr4_2 mRNA 74851369 74853296 74851369 ID=Msa0622420-mRNA-1;Parent=Msa0622420;Name=Msa0622420-mRNA-1;_AED=0.32;_eAED=0.32;_QI=496|1|1|1|0.66|0.5|4|509|58
chr4_2 exon 74851369 74851733 74851369 ID=Msa0622420-mRNA-1:exon:22663;Parent=Msa0622420-mRNA-1
chr4_2 exon 74852155 74852314 74852155 ID=Msa0622420-mRNA-1:exon:22664;Parent=Msa0622420-mRNA-1
chr4_2 exon 74852521 74852572 74852521 ID=Msa0622420-mRNA-1:exon:22665;Parent=Msa0622420-mRNA-1
chr4_2 exon 74852692 74853296 74852692 ID=Msa0622420-mRNA-1:exon:22666;Parent=Msa0622420-mRNA-1
chr4_2 five_prime_UTR 74851369 74851733 74851369 ID=Msa0622420-mRNA-1:five_prime_utr;Parent=Msa0622420-mRNA-1
chr4_2 five_prime_UTR 74852155 74852285 74852155 ID=Msa0622420-mRNA-1:five_prime_utr;Parent=Msa0622420-mRNA-1
chr4_2 CDS 74852286 74852314 74852286 ID=Msa0622420-mRNA-1:cds;Parent=Msa0622420-mRNA-1
chr4_2 CDS 74852521 74852572 74852521 ID=Msa0622420-mRNA-1:cds;Parent=Msa0622420-mRNA-1
chr4_2 CDS 74852692 74852787 74852692 ID=Msa0622420-mRNA-1:cds;Parent=Msa0622420-mRNA-1
chr4_2 three_prime_UTR 74852788 74853296 74852788 ID=Msa0622420-mRNA-1:three_prime_utr;Parent=Msa0622420-mRNA-1
Gene Sequence

>Msa0622420

ATGGCTACATCCACACCGCCTTCACATCGGAGGAGAACAAGTCCTATAAGGCACGCTAAGGGTGGTTGGACAGCAGAAGAGGATGAGACATTAAGGAATGCCGTTACAGCTTTTAAGGGAAAACATTGGAAGAAAATAGGTAACTATTTGCCTGAGCTAATGCATTTGACAAATTAA
Protein sequence

>Msa0622420

MATSTPPSHRRRTSPIRHAKGGWTAEEDETLRNAVTAFKGKHWKKIGNYLPELMHLTN*