AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0991170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0991170 MtrunA17_Chr7g0238631 78.431 51 11 0 2 52 43 93 2.64e-22 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0991170 AT2G13960.1 65.116 43 15 0 10 52 65 107 2.13e-15 65.5
Msa0991170 AT2G13960.2 65.116 43 15 0 10 52 30 72 2.75e-15 64.3
Msa0991170 AT5G02320.1 65.116 43 15 0 10 52 65 107 2.78e-14 65.1
Msa0991170 AT5G02320.2 65.116 43 15 0 10 52 65 107 2.78e-14 65.1
Msa0991170 AT5G02320.3 65.116 43 15 0 10 52 5 47 3.82e-14 64.7
Msa0991170 AT3G09370.2 61.224 49 16 1 7 52 67 115 1.13e-13 63.5
Msa0991170 AT3G09370.1 56.604 53 20 1 7 56 62 114 1.14e-13 63.5
Msa0991170 AT3G09370.3 61.224 49 16 1 7 52 75 123 1.15e-13 63.5

Find 61 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTAATTGGTCAATTATT+TGG 0.166929 6_4:-54879486 None:intergenic
CCACCCTTAGCGTGCCTTAT+AGG 0.202151 6_4:-54880644 None:intergenic
TATTGTAGGATGAGACATTA+AGG 0.293653 6_4:+54880795 Msa0991170:intron
TGGATTTATGTTGGTGGAGG+TGG 0.309935 6_4:-54879633 None:intergenic
GGAAAACATTGGAAGAAAAT+AGG 0.311225 6_4:+54880839 Msa0991170:CDS
TGTTTGGGCTTCTCTCTAAT+TGG 0.328403 6_4:-54879498 None:intergenic
CTCATCTTCAAATCATATCT+AGG 0.331174 6_4:+54879820 Msa0991170:five_prime_UTR
AGTGTGAGGGAGAAGATATC+AGG 0.349806 6_4:-54879659 None:intergenic
TTGTAATCATGTCTCATAAC+TGG 0.356897 6_4:+54881335 Msa0991170:three_prime_UTR
TGCCACTTCAATAGAAAGAT+TGG 0.375619 6_4:-54880997 None:intergenic
GGATATGGATGTTTGTTGTT+TGG 0.389621 6_4:+54880285 Msa0991170:five_prime_UTR
ATTGAAGTGGCAAGGATGAT+TGG 0.406073 6_4:+54881008 Msa0991170:three_prime_UTR
GGAGGAGAACAAGTCCTATA+AGG 0.409948 6_4:+54880630 Msa0991170:intron
TTTGCCTCTGCACTTTGAGC+TGG 0.415507 6_4:+54881285 Msa0991170:three_prime_UTR
GTAATGTTTCATACATTTCA+AGG 0.423379 6_4:-54881184 None:intergenic
AGGGAGAAGATATCAGGGTT+TGG 0.428253 6_4:-54879653 None:intergenic
AGGGTTTGGATTTATGTTGG+TGG 0.431049 6_4:-54879639 None:intergenic
AATTGAAAACCGCATCTTGA+AGG 0.449911 6_4:-54879771 None:intergenic
AGTATAATTCAAAAGTCTCC+AGG 0.458256 6_4:+54880360 Msa0991170:five_prime_UTR
TTTGTCAAATGCATTAGCTC+AGG 0.463353 6_4:-54880872 None:intergenic
ATCAGGGTTTGGATTTATGT+TGG 0.464167 6_4:-54879642 None:intergenic
TTTGTGAATCGGAGAAGAAA+GGG 0.464883 6_4:-54879744 None:intergenic
GTCCAATCTTTCTATTGAAG+TGG 0.468597 6_4:+54880995 Msa0991170:three_prime_UTR
AGAAAGGGAGGAGGTTCAGA+TGG 0.474386 6_4:-54879729 None:intergenic
AGGCGGTGTGGATGTAGCCA+TGG 0.485223 6_4:-54880394 None:intergenic
GTTTGGATTTATGTTGGTGG+AGG 0.500329 6_4:-54879636 None:intergenic
GGCGGTGTGGATGTAGCCAT+GGG 0.512625 6_4:-54880393 None:intergenic
GAAGGAGAGAAATGTTACAT+AGG 0.517797 6_4:-54879574 None:intergenic
GAGGGAAGGAAGATAAAGAA+GGG 0.520886 6_4:-54879688 None:intergenic
GAAATGTTACATAGGAAATG+TGG 0.521689 6_4:-54879566 None:intergenic
GTGTGAGGGAGAAGATATCA+GGG 0.524168 6_4:-54879658 None:intergenic
TGAGGGAAGGAAGATAAAGA+AGG 0.525432 6_4:-54879689 None:intergenic
AAGTCCTATAAGGCACGCTA+AGG 0.528953 6_4:+54880640 Msa0991170:CDS
TCATCTTCAAATCATATCTA+GGG 0.535900 6_4:+54879821 Msa0991170:five_prime_UTR
GATTATGAAGTAAAGACAAA+AGG 0.537647 6_4:-54881035 None:intergenic
ATCTTTCTATTGAAGTGGCA+AGG 0.545324 6_4:+54881000 Msa0991170:three_prime_UTR
GCCATGGGGCTACATATCCC+TGG 0.552664 6_4:-54880378 None:intergenic
TCTTGCTCATCTGTGTCTGA+AGG 0.559484 6_4:+54880330 Msa0991170:five_prime_UTR
ACAAAATCTCCTTCAAGATG+CGG 0.561408 6_4:+54879762 Msa0991170:five_prime_UTR
GTGAATCGGAGAAGAAAGGG+AGG 0.561423 6_4:-54879741 None:intergenic
TAAGGCACGCTAAGGGTGGT+TGG 0.562887 6_4:+54880648 Msa0991170:CDS
AAAGAGAAGCATGTGAGGGA+AGG 0.591322 6_4:-54879702 None:intergenic
AACAAACATTACCGATGTGA+AGG 0.591884 6_4:-54880414 None:intergenic
AGAAGGGGAAAATAGTGTGA+GGG 0.595581 6_4:-54879672 None:intergenic
GTGGCAAAGAGAAGCATGTG+AGG 0.601561 6_4:-54879707 None:intergenic
GGGTGGTTGGACAGCAGAAG+AGG 0.605706 6_4:+54880661 Msa0991170:CDS
AGGGAAGGAAGATAAAGAAG+GGG 0.607295 6_4:-54879687 None:intergenic
CCTATAAGGCACGCTAAGGG+TGG 0.618742 6_4:+54880644 Msa0991170:CDS
AGTCCTATAAGGCACGCTAA+GGG 0.620051 6_4:+54880641 Msa0991170:CDS
AATCGGAGAAGAAAGGGAGG+AGG 0.620302 6_4:-54879738 None:intergenic
AAGAAGGGGAAAATAGTGTG+AGG 0.621252 6_4:-54879673 None:intergenic
AAGGGAGGAGGTTCAGATGG+TGG 0.627661 6_4:-54879726 None:intergenic
ACAAACAAAATCAATAGCAG+AGG 0.629915 6_4:-54881316 None:intergenic
GTATAATTCAAAAGTCTCCA+GGG 0.641290 6_4:+54880361 Msa0991170:five_prime_UTR
TCCAGGGATATGTAGCCCCA+TGG 0.641883 6_4:+54880377 Msa0991170:exon
ATGTCCAGCTCAAAGTGCAG+AGG 0.663657 6_4:-54881289 None:intergenic
CATCCACACCGCCTTCACAT+CGG 0.670345 6_4:+54880403 Msa0991170:CDS
TGGCAAAGAGAAGCATGTGA+GGG 0.676512 6_4:-54879706 None:intergenic
AAACATTACCGATGTGAAGG+CGG 0.687678 6_4:-54880411 None:intergenic
TTACCGATGTGAAGGCGGTG+TGG 0.693836 6_4:-54880406 None:intergenic
GCGGTGTGGATGTAGCCATG+GGG 0.784527 6_4:-54880392 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTAGTAATTAAAGA+TGG - chr6_4:54879911-54879930 None:intergenic 10.0%
!!! ATTTTTTTTTTTTTATACTG+AGG + chr6_4:54880197-54880216 Msa0991170:intron 10.0%
!! AAATAATTAGTAATTAAAGA+TGG - chr6_4:54879911-54879930 None:intergenic 10.0%
!!! ATTTTTTTTTTTTTATACTG+AGG + chr6_4:54880197-54880216 Msa0991170:intron 10.0%
!! AATATAAATGCATTGAATAA+AGG - chr6_4:54879861-54879880 None:intergenic 15.0%
!!! TTTTTTTATTGGTTTTGTTT+TGG + chr6_4:54880071-54880090 Msa0991170:intron 15.0%
!!! GATAAAGATTTTGTTTTTTT+GGG + chr6_4:54880093-54880112 Msa0991170:intron 15.0%
!! AATATAAATGCATTGAATAA+AGG - chr6_4:54879861-54879880 None:intergenic 15.0%
!!! TTTTTTTATTGGTTTTGTTT+TGG + chr6_4:54880071-54880090 Msa0991170:intron 15.0%
!!! GATAAAGATTTTGTTTTTTT+GGG + chr6_4:54880093-54880112 Msa0991170:intron 15.0%
!!! GTCAAAAAACGTTTTTTTAT+TGG + chr6_4:54880060-54880079 Msa0991170:intron 20.0%
!!! GGATAAAGATTTTGTTTTTT+TGG + chr6_4:54880092-54880111 Msa0991170:intron 20.0%
!!! TTTTTTTGGGAAAAAATTGA+AGG + chr6_4:54880106-54880125 Msa0991170:intron 20.0%
!!! TTTTTTTTTTATACTGAGGA+TGG + chr6_4:54880201-54880220 Msa0991170:intron 20.0%
!!! TTTTTAGGGTTTTTTATGTT+TGG + chr6_4:54880224-54880243 Msa0991170:intron 20.0%
!!! TGTTATGTTATTTACGTTTT+TGG + chr6_4:54880433-54880452 Msa0991170:intron 20.0%
!!! CATTTTAATTAGAAAGATTG+AGG + chr6_4:54881072-54881091 Msa0991170:three_prime_UTR 20.0%
!!! GTCAAAAAACGTTTTTTTAT+TGG + chr6_4:54880060-54880079 Msa0991170:intron 20.0%
!!! GGATAAAGATTTTGTTTTTT+TGG + chr6_4:54880092-54880111 Msa0991170:intron 20.0%
!!! TTTTTTTGGGAAAAAATTGA+AGG + chr6_4:54880106-54880125 Msa0991170:intron 20.0%
!!! TTTTTTTTTTATACTGAGGA+TGG + chr6_4:54880201-54880220 Msa0991170:intron 20.0%
!!! TTTTTAGGGTTTTTTATGTT+TGG + chr6_4:54880224-54880243 Msa0991170:intron 20.0%
!!! TGTTATGTTATTTACGTTTT+TGG + chr6_4:54880433-54880452 Msa0991170:intron 20.0%
!!! CATTTTAATTAGAAAGATTG+AGG + chr6_4:54881072-54881091 Msa0991170:three_prime_UTR 20.0%
! TCTCTAATTGGTCAATTATT+TGG - chr6_4:54879489-54879508 None:intergenic 25.0%
! TCATCTTCAAATCATATCTA+GGG + chr6_4:54879821-54879840 Msa0991170:five_prime_UTR 25.0%
!! TTGATTGAGTTTTTGTGTTT+TGG + chr6_4:54880014-54880033 Msa0991170:intron 25.0%
!!! AGTTTTTGTGTTTTGGATAA+AGG + chr6_4:54880021-54880040 Msa0991170:intron 25.0%
!!! GTTTTTGTGTTTTGGATAAA+GGG + chr6_4:54880022-54880041 Msa0991170:intron 25.0%
!!! TTTTTGTGTTTTGGATAAAG+GGG + chr6_4:54880023-54880042 Msa0991170:intron 25.0%
!!! TGAAGGTTTTGATTTGATTA+TGG + chr6_4:54880123-54880142 Msa0991170:intron 25.0%
!!! TTTTTATGTTTGGTGATTGA+TGG + chr6_4:54880234-54880253 Msa0991170:intron 25.0%
!!! TTTTATGTTTGGTGATTGAT+GGG + chr6_4:54880235-54880254 Msa0991170:intron 25.0%
! ATTGAAGAACACAATAAGAA+CGG - chr6_4:54880774-54880793 None:intergenic 25.0%
! TGTGTTCTTCAATTTATTGT+AGG + chr6_4:54880781-54880800 Msa0991170:intron 25.0%
! GATTATGAAGTAAAGACAAA+AGG - chr6_4:54881038-54881057 None:intergenic 25.0%
! GTAATGTTTCATACATTTCA+AGG - chr6_4:54881187-54881206 None:intergenic 25.0%
!!! GTGTTTTTTGTTGATTTTGT+TGG + chr6_4:54881254-54881273 Msa0991170:three_prime_UTR 25.0%
! TCTCTAATTGGTCAATTATT+TGG - chr6_4:54879489-54879508 None:intergenic 25.0%
! TCATCTTCAAATCATATCTA+GGG + chr6_4:54879821-54879840 Msa0991170:five_prime_UTR 25.0%
!! TTGATTGAGTTTTTGTGTTT+TGG + chr6_4:54880014-54880033 Msa0991170:intron 25.0%
!!! AGTTTTTGTGTTTTGGATAA+AGG + chr6_4:54880021-54880040 Msa0991170:intron 25.0%
!!! GTTTTTGTGTTTTGGATAAA+GGG + chr6_4:54880022-54880041 Msa0991170:intron 25.0%
!!! TTTTTGTGTTTTGGATAAAG+GGG + chr6_4:54880023-54880042 Msa0991170:intron 25.0%
!!! TGAAGGTTTTGATTTGATTA+TGG + chr6_4:54880123-54880142 Msa0991170:intron 25.0%
!!! TTTTTATGTTTGGTGATTGA+TGG + chr6_4:54880234-54880253 Msa0991170:intron 25.0%
!!! TTTTATGTTTGGTGATTGAT+GGG + chr6_4:54880235-54880254 Msa0991170:intron 25.0%
! ATTGAAGAACACAATAAGAA+CGG - chr6_4:54880774-54880793 None:intergenic 25.0%
! TGTGTTCTTCAATTTATTGT+AGG + chr6_4:54880781-54880800 Msa0991170:intron 25.0%
! GATTATGAAGTAAAGACAAA+AGG - chr6_4:54881038-54881057 None:intergenic 25.0%
! GTAATGTTTCATACATTTCA+AGG - chr6_4:54881187-54881206 None:intergenic 25.0%
!!! GTGTTTTTTGTTGATTTTGT+TGG + chr6_4:54881254-54881273 Msa0991170:three_prime_UTR 25.0%
! TTTTGCTTGTAGTCATGTTT+GGG - chr6_4:54879516-54879535 None:intergenic 30.0%
! TTTTTGCTTGTAGTCATGTT+TGG - chr6_4:54879517-54879536 None:intergenic 30.0%
GAAATGTTACATAGGAAATG+TGG - chr6_4:54879569-54879588 None:intergenic 30.0%
!! TTTTGAAAAGAGAGAAAGGA+AGG - chr6_4:54879595-54879614 None:intergenic 30.0%
! CCTTTCTCTCTTTTCAAAAT+CGG + chr6_4:54879596-54879615 Msa0991170:five_prime_UTR 30.0%
! TTGAAGGAGATTTTGTGAAT+CGG - chr6_4:54879758-54879777 None:intergenic 30.0%
CTCATCTTCAAATCATATCT+AGG + chr6_4:54879820-54879839 Msa0991170:five_prime_UTR 30.0%
TCGAAGAATTTCAAGATTTC+TGG + chr6_4:54879974-54879993 Msa0991170:intron 30.0%
!!! TAAAGGGGTTTGATTTGTTT+TGG + chr6_4:54880038-54880057 Msa0991170:intron 30.0%
!!! TGATTATGGATTTTGAGATC+TGG + chr6_4:54880137-54880156 Msa0991170:intron 30.0%
!!! GATTATGGATTTTGAGATCT+GGG + chr6_4:54880138-54880157 Msa0991170:intron 30.0%
! TGAGTAAAAGTGTGAAAGTT+TGG + chr6_4:54880174-54880193 Msa0991170:intron 30.0%
! TTATACTGAGGATGGTTTTT+AGG + chr6_4:54880209-54880228 Msa0991170:intron 30.0%
! TATACTGAGGATGGTTTTTA+GGG + chr6_4:54880210-54880229 Msa0991170:intron 30.0%
!! TTTTTAGGGATTGAAGGTTT+TGG + chr6_4:54880258-54880277 Msa0991170:intron 30.0%
AGTATAATTCAAAAGTCTCC+AGG + chr6_4:54880360-54880379 Msa0991170:five_prime_UTR 30.0%
GTATAATTCAAAAGTCTCCA+GGG + chr6_4:54880361-54880380 Msa0991170:five_prime_UTR 30.0%
TTTGGTTGCAGTTGTAATTA+TGG + chr6_4:54880451-54880470 Msa0991170:intron 30.0%
! TTTTATGCCAAATCTGCTAT+TGG + chr6_4:54880510-54880529 Msa0991170:intron 30.0%
AATTACACCAATAGCAGATT+TGG - chr6_4:54880520-54880539 None:intergenic 30.0%
ACCATATGATATGTTTGTAC+AGG + chr6_4:54880609-54880628 Msa0991170:intron 30.0%
TCCTGTACAAACATATCATA+TGG - chr6_4:54880613-54880632 None:intergenic 30.0%
ATATGATATGTTTGTACAGG+AGG + chr6_4:54880612-54880631 Msa0991170:intron 30.0%
AGAGGTTCATATTTGCTTAT+TGG + chr6_4:54880679-54880698 Msa0991170:intron 30.0%
TATTGTAGGATGAGACATTA+AGG + chr6_4:54880795-54880814 Msa0991170:intron 30.0%
! TTTTCCCTTAAAAGCTGTAA+CGG - chr6_4:54880825-54880844 None:intergenic 30.0%
GGAAAACATTGGAAGAAAAT+AGG + chr6_4:54880839-54880858 Msa0991170:CDS 30.0%
!!! TTGTTGATTTTGTTGGCTAT+TGG + chr6_4:54881261-54881280 Msa0991170:three_prime_UTR 30.0%
ACAAACAAAATCAATAGCAG+AGG - chr6_4:54881319-54881338 None:intergenic 30.0%
TTGTAATCATGTCTCATAAC+TGG + chr6_4:54881335-54881354 Msa0991170:three_prime_UTR 30.0%
! TTTTGCTTGTAGTCATGTTT+GGG - chr6_4:54879516-54879535 None:intergenic 30.0%
! TTTTTGCTTGTAGTCATGTT+TGG - chr6_4:54879517-54879536 None:intergenic 30.0%
GAAATGTTACATAGGAAATG+TGG - chr6_4:54879569-54879588 None:intergenic 30.0%
!! TTTTGAAAAGAGAGAAAGGA+AGG - chr6_4:54879595-54879614 None:intergenic 30.0%
! CCTTTCTCTCTTTTCAAAAT+CGG + chr6_4:54879596-54879615 Msa0991170:five_prime_UTR 30.0%
! TTGAAGGAGATTTTGTGAAT+CGG - chr6_4:54879758-54879777 None:intergenic 30.0%
CTCATCTTCAAATCATATCT+AGG + chr6_4:54879820-54879839 Msa0991170:five_prime_UTR 30.0%
TCGAAGAATTTCAAGATTTC+TGG + chr6_4:54879974-54879993 Msa0991170:intron 30.0%
!!! TAAAGGGGTTTGATTTGTTT+TGG + chr6_4:54880038-54880057 Msa0991170:intron 30.0%
!!! TGATTATGGATTTTGAGATC+TGG + chr6_4:54880137-54880156 Msa0991170:intron 30.0%
!!! GATTATGGATTTTGAGATCT+GGG + chr6_4:54880138-54880157 Msa0991170:intron 30.0%
! TGAGTAAAAGTGTGAAAGTT+TGG + chr6_4:54880174-54880193 Msa0991170:intron 30.0%
! TTATACTGAGGATGGTTTTT+AGG + chr6_4:54880209-54880228 Msa0991170:intron 30.0%
! TATACTGAGGATGGTTTTTA+GGG + chr6_4:54880210-54880229 Msa0991170:intron 30.0%
!! TTTTTAGGGATTGAAGGTTT+TGG + chr6_4:54880258-54880277 Msa0991170:intron 30.0%
AGTATAATTCAAAAGTCTCC+AGG + chr6_4:54880360-54880379 Msa0991170:five_prime_UTR 30.0%
GTATAATTCAAAAGTCTCCA+GGG + chr6_4:54880361-54880380 Msa0991170:five_prime_UTR 30.0%
TTTGGTTGCAGTTGTAATTA+TGG + chr6_4:54880451-54880470 Msa0991170:intron 30.0%
! TTTTATGCCAAATCTGCTAT+TGG + chr6_4:54880510-54880529 Msa0991170:intron 30.0%
AATTACACCAATAGCAGATT+TGG - chr6_4:54880520-54880539 None:intergenic 30.0%
ACCATATGATATGTTTGTAC+AGG + chr6_4:54880609-54880628 Msa0991170:intron 30.0%
TCCTGTACAAACATATCATA+TGG - chr6_4:54880613-54880632 None:intergenic 30.0%
ATATGATATGTTTGTACAGG+AGG + chr6_4:54880612-54880631 Msa0991170:intron 30.0%
AGAGGTTCATATTTGCTTAT+TGG + chr6_4:54880679-54880698 Msa0991170:intron 30.0%
TATTGTAGGATGAGACATTA+AGG + chr6_4:54880795-54880814 Msa0991170:intron 30.0%
! TTTTCCCTTAAAAGCTGTAA+CGG - chr6_4:54880825-54880844 None:intergenic 30.0%
GGAAAACATTGGAAGAAAAT+AGG + chr6_4:54880839-54880858 Msa0991170:CDS 30.0%
!!! TTGTTGATTTTGTTGGCTAT+TGG + chr6_4:54881261-54881280 Msa0991170:three_prime_UTR 30.0%
ACAAACAAAATCAATAGCAG+AGG - chr6_4:54881319-54881338 None:intergenic 30.0%
TTGTAATCATGTCTCATAAC+TGG + chr6_4:54881335-54881354 Msa0991170:three_prime_UTR 30.0%
GAAGGAGAGAAATGTTACAT+AGG - chr6_4:54879577-54879596 None:intergenic 35.0%
!! CCGATTTTGAAAAGAGAGAA+AGG - chr6_4:54879599-54879618 None:intergenic 35.0%
ATCAGGGTTTGGATTTATGT+TGG - chr6_4:54879645-54879664 None:intergenic 35.0%
TTTGTGAATCGGAGAAGAAA+GGG - chr6_4:54879747-54879766 None:intergenic 35.0%
! TTTTGTGAATCGGAGAAGAA+AGG - chr6_4:54879748-54879767 None:intergenic 35.0%
ACAAAATCTCCTTCAAGATG+CGG + chr6_4:54879762-54879781 Msa0991170:five_prime_UTR 35.0%
AATTGAAAACCGCATCTTGA+AGG - chr6_4:54879774-54879793 None:intergenic 35.0%
! TCAAGATGCGGTTTTCAATT+CGG + chr6_4:54879774-54879793 Msa0991170:five_prime_UTR 35.0%
! TGAAATTCTTCGAAGAGTGT+TGG - chr6_4:54879967-54879986 None:intergenic 35.0%
!!! TTGGTGATTGATGGGTTTTT+AGG + chr6_4:54880243-54880262 Msa0991170:intron 35.0%
!!! TGGTGATTGATGGGTTTTTA+GGG + chr6_4:54880244-54880263 Msa0991170:intron 35.0%
!! GGATATGGATGTTTGTTGTT+TGG + chr6_4:54880285-54880304 Msa0991170:five_prime_UTR 35.0%
AACAAACATTACCGATGTGA+AGG - chr6_4:54880417-54880436 None:intergenic 35.0%
!! AATAGTTGCTGATTCACTTC+TGG + chr6_4:54880551-54880570 Msa0991170:intron 35.0%
!! ATTCAATCCACTTTTCTGGA+AGG - chr6_4:54880726-54880745 None:intergenic 35.0%
!! TCCAGAAAAGTGGATTGAAT+TGG + chr6_4:54880726-54880745 Msa0991170:intron 35.0%
!! CCAGAAAAGTGGATTGAATT+GGG + chr6_4:54880727-54880746 Msa0991170:intron 35.0%
! AATGCCGTTACAGCTTTTAA+GGG + chr6_4:54880818-54880837 Msa0991170:CDS 35.0%
!! CAGCTTTTAAGGGAAAACAT+TGG + chr6_4:54880828-54880847 Msa0991170:CDS 35.0%
TTTGTCAAATGCATTAGCTC+AGG - chr6_4:54880875-54880894 None:intergenic 35.0%
GTCCAATCTTTCTATTGAAG+TGG + chr6_4:54880995-54881014 Msa0991170:three_prime_UTR 35.0%
! TGCCACTTCAATAGAAAGAT+TGG - chr6_4:54881000-54881019 None:intergenic 35.0%
ATCTTTCTATTGAAGTGGCA+AGG + chr6_4:54881000-54881019 Msa0991170:three_prime_UTR 35.0%
GAAGGAGAGAAATGTTACAT+AGG - chr6_4:54879577-54879596 None:intergenic 35.0%
!! CCGATTTTGAAAAGAGAGAA+AGG - chr6_4:54879599-54879618 None:intergenic 35.0%
ATCAGGGTTTGGATTTATGT+TGG - chr6_4:54879645-54879664 None:intergenic 35.0%
TTTGTGAATCGGAGAAGAAA+GGG - chr6_4:54879747-54879766 None:intergenic 35.0%
! TTTTGTGAATCGGAGAAGAA+AGG - chr6_4:54879748-54879767 None:intergenic 35.0%
ACAAAATCTCCTTCAAGATG+CGG + chr6_4:54879762-54879781 Msa0991170:five_prime_UTR 35.0%
AATTGAAAACCGCATCTTGA+AGG - chr6_4:54879774-54879793 None:intergenic 35.0%
! TCAAGATGCGGTTTTCAATT+CGG + chr6_4:54879774-54879793 Msa0991170:five_prime_UTR 35.0%
! TGAAATTCTTCGAAGAGTGT+TGG - chr6_4:54879967-54879986 None:intergenic 35.0%
!!! TTGGTGATTGATGGGTTTTT+AGG + chr6_4:54880243-54880262 Msa0991170:intron 35.0%
!!! TGGTGATTGATGGGTTTTTA+GGG + chr6_4:54880244-54880263 Msa0991170:intron 35.0%
!! GGATATGGATGTTTGTTGTT+TGG + chr6_4:54880285-54880304 Msa0991170:five_prime_UTR 35.0%
AACAAACATTACCGATGTGA+AGG - chr6_4:54880417-54880436 None:intergenic 35.0%
!! AATAGTTGCTGATTCACTTC+TGG + chr6_4:54880551-54880570 Msa0991170:intron 35.0%
!! ATTCAATCCACTTTTCTGGA+AGG - chr6_4:54880726-54880745 None:intergenic 35.0%
!! TCCAGAAAAGTGGATTGAAT+TGG + chr6_4:54880726-54880745 Msa0991170:intron 35.0%
!! CCAGAAAAGTGGATTGAATT+GGG + chr6_4:54880727-54880746 Msa0991170:intron 35.0%
! AATGCCGTTACAGCTTTTAA+GGG + chr6_4:54880818-54880837 Msa0991170:CDS 35.0%
!! CAGCTTTTAAGGGAAAACAT+TGG + chr6_4:54880828-54880847 Msa0991170:CDS 35.0%
TTTGTCAAATGCATTAGCTC+AGG - chr6_4:54880875-54880894 None:intergenic 35.0%
GTCCAATCTTTCTATTGAAG+TGG + chr6_4:54880995-54881014 Msa0991170:three_prime_UTR 35.0%
! TGCCACTTCAATAGAAAGAT+TGG - chr6_4:54881000-54881019 None:intergenic 35.0%
ATCTTTCTATTGAAGTGGCA+AGG + chr6_4:54881000-54881019 Msa0991170:three_prime_UTR 35.0%
TGTTTGGGCTTCTCTCTAAT+TGG - chr6_4:54879501-54879520 None:intergenic 40.0%
! GTTTGGATTTATGTTGGTGG+AGG - chr6_4:54879639-54879658 None:intergenic 40.0%
AGGGTTTGGATTTATGTTGG+TGG - chr6_4:54879642-54879661 None:intergenic 40.0%
AGAAGGGGAAAATAGTGTGA+GGG - chr6_4:54879675-54879694 None:intergenic 40.0%
AAGAAGGGGAAAATAGTGTG+AGG - chr6_4:54879676-54879695 None:intergenic 40.0%
AGGGAAGGAAGATAAAGAAG+GGG - chr6_4:54879690-54879709 None:intergenic 40.0%
GAGGGAAGGAAGATAAAGAA+GGG - chr6_4:54879691-54879710 None:intergenic 40.0%
TGAGGGAAGGAAGATAAAGA+AGG - chr6_4:54879692-54879711 None:intergenic 40.0%
!! GATGGGTTTTTAGGGATTGA+AGG + chr6_4:54880252-54880271 Msa0991170:intron 40.0%
!! GAAGGTTTTGGTGAAGGATA+TGG + chr6_4:54880270-54880289 Msa0991170:five_prime_UTR 40.0%
AAACATTACCGATGTGAAGG+CGG - chr6_4:54880414-54880433 None:intergenic 40.0%
! TTTTCCTCCTTCCAGAAAAG+TGG + chr6_4:54880716-54880735 Msa0991170:intron 40.0%
!! CCCAATTCAATCCACTTTTC+TGG - chr6_4:54880730-54880749 None:intergenic 40.0%
! GAATGCCGTTACAGCTTTTA+AGG + chr6_4:54880817-54880836 Msa0991170:CDS 40.0%
ATTGAAGTGGCAAGGATGAT+TGG + chr6_4:54881008-54881027 Msa0991170:three_prime_UTR 40.0%
TGTTTGGGCTTCTCTCTAAT+TGG - chr6_4:54879501-54879520 None:intergenic 40.0%
! GTTTGGATTTATGTTGGTGG+AGG - chr6_4:54879639-54879658 None:intergenic 40.0%
AGGGTTTGGATTTATGTTGG+TGG - chr6_4:54879642-54879661 None:intergenic 40.0%
AGAAGGGGAAAATAGTGTGA+GGG - chr6_4:54879675-54879694 None:intergenic 40.0%
AAGAAGGGGAAAATAGTGTG+AGG - chr6_4:54879676-54879695 None:intergenic 40.0%
AGGGAAGGAAGATAAAGAAG+GGG - chr6_4:54879690-54879709 None:intergenic 40.0%
GAGGGAAGGAAGATAAAGAA+GGG - chr6_4:54879691-54879710 None:intergenic 40.0%
TGAGGGAAGGAAGATAAAGA+AGG - chr6_4:54879692-54879711 None:intergenic 40.0%
!! GATGGGTTTTTAGGGATTGA+AGG + chr6_4:54880252-54880271 Msa0991170:intron 40.0%
!! GAAGGTTTTGGTGAAGGATA+TGG + chr6_4:54880270-54880289 Msa0991170:five_prime_UTR 40.0%
AAACATTACCGATGTGAAGG+CGG - chr6_4:54880414-54880433 None:intergenic 40.0%
! TTTTCCTCCTTCCAGAAAAG+TGG + chr6_4:54880716-54880735 Msa0991170:intron 40.0%
!! CCCAATTCAATCCACTTTTC+TGG - chr6_4:54880730-54880749 None:intergenic 40.0%
! GAATGCCGTTACAGCTTTTA+AGG + chr6_4:54880817-54880836 Msa0991170:CDS 40.0%
ATTGAAGTGGCAAGGATGAT+TGG + chr6_4:54881008-54881027 Msa0991170:three_prime_UTR 40.0%
! TGGATTTATGTTGGTGGAGG+TGG - chr6_4:54879636-54879655 None:intergenic 45.0%
AGGGAGAAGATATCAGGGTT+TGG - chr6_4:54879656-54879675 None:intergenic 45.0%
GTGTGAGGGAGAAGATATCA+GGG - chr6_4:54879661-54879680 None:intergenic 45.0%
AGTGTGAGGGAGAAGATATC+AGG - chr6_4:54879662-54879681 None:intergenic 45.0%
! AAAGAGAAGCATGTGAGGGA+AGG - chr6_4:54879705-54879724 None:intergenic 45.0%
! TGGCAAAGAGAAGCATGTGA+GGG - chr6_4:54879709-54879728 None:intergenic 45.0%
!! GGGATTGAAGGTTTTGGTGA+AGG + chr6_4:54880264-54880283 Msa0991170:intron 45.0%
TCTTGCTCATCTGTGTCTGA+AGG + chr6_4:54880330-54880349 Msa0991170:five_prime_UTR 45.0%
GGAGGAGAACAAGTCCTATA+AGG + chr6_4:54880630-54880649 Msa0991170:intron 45.0%
AAGTCCTATAAGGCACGCTA+AGG + chr6_4:54880640-54880659 Msa0991170:CDS 45.0%
AGTCCTATAAGGCACGCTAA+GGG + chr6_4:54880641-54880660 Msa0991170:CDS 45.0%
!! CAATCCACTTTTCTGGAAGG+AGG - chr6_4:54880723-54880742 None:intergenic 45.0%
! TGGATTTATGTTGGTGGAGG+TGG - chr6_4:54879636-54879655 None:intergenic 45.0%
AGGGAGAAGATATCAGGGTT+TGG - chr6_4:54879656-54879675 None:intergenic 45.0%
GTGTGAGGGAGAAGATATCA+GGG - chr6_4:54879661-54879680 None:intergenic 45.0%
AGTGTGAGGGAGAAGATATC+AGG - chr6_4:54879662-54879681 None:intergenic 45.0%
! AAAGAGAAGCATGTGAGGGA+AGG - chr6_4:54879705-54879724 None:intergenic 45.0%
! TGGCAAAGAGAAGCATGTGA+GGG - chr6_4:54879709-54879728 None:intergenic 45.0%
!! GGGATTGAAGGTTTTGGTGA+AGG + chr6_4:54880264-54880283 Msa0991170:intron 45.0%
TCTTGCTCATCTGTGTCTGA+AGG + chr6_4:54880330-54880349 Msa0991170:five_prime_UTR 45.0%
GGAGGAGAACAAGTCCTATA+AGG + chr6_4:54880630-54880649 Msa0991170:intron 45.0%
AAGTCCTATAAGGCACGCTA+AGG + chr6_4:54880640-54880659 Msa0991170:CDS 45.0%
AGTCCTATAAGGCACGCTAA+GGG + chr6_4:54880641-54880660 Msa0991170:CDS 45.0%
!! CAATCCACTTTTCTGGAAGG+AGG - chr6_4:54880723-54880742 None:intergenic 45.0%
! GTGGCAAAGAGAAGCATGTG+AGG - chr6_4:54879710-54879729 None:intergenic 50.0%
AGAAAGGGAGGAGGTTCAGA+TGG - chr6_4:54879732-54879751 None:intergenic 50.0%
AATCGGAGAAGAAAGGGAGG+AGG - chr6_4:54879741-54879760 None:intergenic 50.0%
GTGAATCGGAGAAGAAAGGG+AGG - chr6_4:54879744-54879763 None:intergenic 50.0%
TTTGCCTCTGCACTTTGAGC+TGG + chr6_4:54881285-54881304 Msa0991170:three_prime_UTR 50.0%
ATGTCCAGCTCAAAGTGCAG+AGG - chr6_4:54881292-54881311 None:intergenic 50.0%
! GTGGCAAAGAGAAGCATGTG+AGG - chr6_4:54879710-54879729 None:intergenic 50.0%
AGAAAGGGAGGAGGTTCAGA+TGG - chr6_4:54879732-54879751 None:intergenic 50.0%
AATCGGAGAAGAAAGGGAGG+AGG - chr6_4:54879741-54879760 None:intergenic 50.0%
GTGAATCGGAGAAGAAAGGG+AGG - chr6_4:54879744-54879763 None:intergenic 50.0%
TTTGCCTCTGCACTTTGAGC+TGG + chr6_4:54881285-54881304 Msa0991170:three_prime_UTR 50.0%
ATGTCCAGCTCAAAGTGCAG+AGG - chr6_4:54881292-54881311 None:intergenic 50.0%
AAGGGAGGAGGTTCAGATGG+TGG - chr6_4:54879729-54879748 None:intergenic 55.0%
TCCAGGGATATGTAGCCCCA+TGG + chr6_4:54880377-54880396 Msa0991170:exon 55.0%
CATCCACACCGCCTTCACAT+CGG + chr6_4:54880403-54880422 Msa0991170:CDS 55.0%
TTACCGATGTGAAGGCGGTG+TGG - chr6_4:54880409-54880428 None:intergenic 55.0%
CCACCCTTAGCGTGCCTTAT+AGG - chr6_4:54880647-54880666 None:intergenic 55.0%
CCTATAAGGCACGCTAAGGG+TGG + chr6_4:54880644-54880663 Msa0991170:CDS 55.0%
TAAGGCACGCTAAGGGTGGT+TGG + chr6_4:54880648-54880667 Msa0991170:CDS 55.0%
AAGGGAGGAGGTTCAGATGG+TGG - chr6_4:54879729-54879748 None:intergenic 55.0%
TCCAGGGATATGTAGCCCCA+TGG + chr6_4:54880377-54880396 Msa0991170:exon 55.0%
CATCCACACCGCCTTCACAT+CGG + chr6_4:54880403-54880422 Msa0991170:CDS 55.0%
TTACCGATGTGAAGGCGGTG+TGG - chr6_4:54880409-54880428 None:intergenic 55.0%
CCACCCTTAGCGTGCCTTAT+AGG - chr6_4:54880647-54880666 None:intergenic 55.0%
CCTATAAGGCACGCTAAGGG+TGG + chr6_4:54880644-54880663 Msa0991170:CDS 55.0%
TAAGGCACGCTAAGGGTGGT+TGG + chr6_4:54880648-54880667 Msa0991170:CDS 55.0%
GCCATGGGGCTACATATCCC+TGG - chr6_4:54880381-54880400 None:intergenic 60.0%
GCGGTGTGGATGTAGCCATG+GGG - chr6_4:54880395-54880414 None:intergenic 60.0%
GGCGGTGTGGATGTAGCCAT+GGG - chr6_4:54880396-54880415 None:intergenic 60.0%
AGGCGGTGTGGATGTAGCCA+TGG - chr6_4:54880397-54880416 None:intergenic 60.0%
GGGTGGTTGGACAGCAGAAG+AGG + chr6_4:54880661-54880680 Msa0991170:CDS 60.0%
GCCATGGGGCTACATATCCC+TGG - chr6_4:54880381-54880400 None:intergenic 60.0%
GCGGTGTGGATGTAGCCATG+GGG - chr6_4:54880395-54880414 None:intergenic 60.0%
GGCGGTGTGGATGTAGCCAT+GGG - chr6_4:54880396-54880415 None:intergenic 60.0%
AGGCGGTGTGGATGTAGCCA+TGG - chr6_4:54880397-54880416 None:intergenic 60.0%
GGGTGGTTGGACAGCAGAAG+AGG + chr6_4:54880661-54880680 Msa0991170:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 54879478 54881407 54879478 ID=Msa0991170;Name=Msa0991170
chr6_4 mRNA 54879478 54881407 54879478 ID=Msa0991170-mRNA-1;Parent=Msa0991170;Name=Msa0991170-mRNA-1;_AED=0.32;_eAED=0.32;_QI=496|1|1|1|0.66|0.5|4|509|58
chr6_4 exon 54879478 54879842 54879478 ID=Msa0991170-mRNA-1:exon:11221;Parent=Msa0991170-mRNA-1
chr6_4 exon 54880265 54880424 54880265 ID=Msa0991170-mRNA-1:exon:11222;Parent=Msa0991170-mRNA-1
chr6_4 exon 54880631 54880682 54880631 ID=Msa0991170-mRNA-1:exon:11223;Parent=Msa0991170-mRNA-1
chr6_4 exon 54880803 54881407 54880803 ID=Msa0991170-mRNA-1:exon:11224;Parent=Msa0991170-mRNA-1
chr6_4 five_prime_UTR 54879478 54879842 54879478 ID=Msa0991170-mRNA-1:five_prime_utr;Parent=Msa0991170-mRNA-1
chr6_4 five_prime_UTR 54880265 54880395 54880265 ID=Msa0991170-mRNA-1:five_prime_utr;Parent=Msa0991170-mRNA-1
chr6_4 CDS 54880396 54880424 54880396 ID=Msa0991170-mRNA-1:cds;Parent=Msa0991170-mRNA-1
chr6_4 CDS 54880631 54880682 54880631 ID=Msa0991170-mRNA-1:cds;Parent=Msa0991170-mRNA-1
chr6_4 CDS 54880803 54880898 54880803 ID=Msa0991170-mRNA-1:cds;Parent=Msa0991170-mRNA-1
chr6_4 three_prime_UTR 54880899 54881407 54880899 ID=Msa0991170-mRNA-1:three_prime_utr;Parent=Msa0991170-mRNA-1
Gene Sequence

>Msa0991170

ATGGCTACATCCACACCGCCTTCACATCGGAGGAGAACAAGTCCTATAAGGCACGCTAAGGGTGGTTGGACAGCAGAAGAGGATGAGACATTAAGGAATGCCGTTACAGCTTTTAAGGGAAAACATTGGAAGAAAATAGGTAACTATTTGCCTGAGCTAATGCATTTGACAAATTAA
Protein sequence

>Msa0991170

MATSTPPSHRRRTSPIRHAKGGWTAEEDETLRNAVTAFKGKHWKKIGNYLPELMHLTN*