AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1439180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439180 MtrunA17_Chr7g0238631 78.431 51 11 0 2 52 43 93 2.64e-22 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439180 AT2G13960.1 65.116 43 15 0 10 52 65 107 2.13e-15 65.5
Msa1439180 AT2G13960.2 65.116 43 15 0 10 52 30 72 2.75e-15 64.3
Msa1439180 AT5G02320.1 65.116 43 15 0 10 52 65 107 2.78e-14 65.1
Msa1439180 AT5G02320.2 65.116 43 15 0 10 52 65 107 2.78e-14 65.1
Msa1439180 AT5G02320.3 65.116 43 15 0 10 52 5 47 3.82e-14 64.7
Msa1439180 AT3G09370.2 61.224 49 16 1 7 52 67 115 1.13e-13 63.5
Msa1439180 AT3G09370.1 56.604 53 20 1 7 56 62 114 1.14e-13 63.5
Msa1439180 AT3G09370.3 61.224 49 16 1 7 52 75 123 1.15e-13 63.5

Find 61 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTAATTGGTCAATTATT+TGG 0.166929 tig0028725:-3565 None:intergenic
CCACCCTTAGCGTGCCTTAT+AGG 0.202151 tig0028725:-4722 None:intergenic
TATTGTAGGATGAGACATTA+AGG 0.293653 tig0028725:+4873 Msa1439180:intron
TGGATTTATGTTGGTGGAGG+TGG 0.309935 tig0028725:-3712 None:intergenic
GGAAAACATTGGAAGAAAAT+AGG 0.311225 tig0028725:+4917 Msa1439180:CDS
TGTTTGGGCTTCTCTCTAAT+TGG 0.328403 tig0028725:-3577 None:intergenic
CTCATCTTCAAATCATATCT+AGG 0.331174 tig0028725:+3899 Msa1439180:five_prime_UTR
AGTGTGAGGGAGAAGATATC+AGG 0.349806 tig0028725:-3738 None:intergenic
TTGTAATCATGTCTCATAAC+TGG 0.356897 tig0028725:+5413 Msa1439180:three_prime_UTR
TGCCACTTCAATAGAAAGAT+TGG 0.375619 tig0028725:-5075 None:intergenic
GGATATGGATGTTTGTTGTT+TGG 0.389621 tig0028725:+4363 Msa1439180:five_prime_UTR
ATTGAAGTGGCAAGGATGAT+TGG 0.406073 tig0028725:+5086 Msa1439180:three_prime_UTR
GGAGGAGAACAAGTCCTATA+AGG 0.409948 tig0028725:+4708 Msa1439180:intron
TTTGCCTCTGCACTTTGAGC+TGG 0.415507 tig0028725:+5363 Msa1439180:three_prime_UTR
GTAATGTTTCATACATTTCA+AGG 0.423379 tig0028725:-5262 None:intergenic
AGGGAGAAGATATCAGGGTT+TGG 0.428253 tig0028725:-3732 None:intergenic
AGGGTTTGGATTTATGTTGG+TGG 0.431049 tig0028725:-3718 None:intergenic
AATTGAAAACCGCATCTTGA+AGG 0.449911 tig0028725:-3850 None:intergenic
AGTATAATTCAAAAGTCTCC+AGG 0.458256 tig0028725:+4438 Msa1439180:five_prime_UTR
TTTGTCAAATGCATTAGCTC+AGG 0.463353 tig0028725:-4950 None:intergenic
ATCAGGGTTTGGATTTATGT+TGG 0.464167 tig0028725:-3721 None:intergenic
TTTGTGAATCGGAGAAGAAA+GGG 0.464883 tig0028725:-3823 None:intergenic
GTCCAATCTTTCTATTGAAG+TGG 0.468597 tig0028725:+5073 Msa1439180:three_prime_UTR
AGAAAGGGAGGAGGTTCAGA+TGG 0.474386 tig0028725:-3808 None:intergenic
AGGCGGTGTGGATGTAGCCA+TGG 0.485223 tig0028725:-4472 None:intergenic
GTTTGGATTTATGTTGGTGG+AGG 0.500329 tig0028725:-3715 None:intergenic
GGCGGTGTGGATGTAGCCAT+GGG 0.512625 tig0028725:-4471 None:intergenic
GAAGGAGAGAAATGTTACAT+AGG 0.517797 tig0028725:-3653 None:intergenic
GAGGGAAGGAAGATAAAGAA+GGG 0.520886 tig0028725:-3767 None:intergenic
GAAATGTTACATAGGAAATG+TGG 0.521689 tig0028725:-3645 None:intergenic
GTGTGAGGGAGAAGATATCA+GGG 0.524168 tig0028725:-3737 None:intergenic
TGAGGGAAGGAAGATAAAGA+AGG 0.525432 tig0028725:-3768 None:intergenic
AAGTCCTATAAGGCACGCTA+AGG 0.528953 tig0028725:+4718 Msa1439180:CDS
TCATCTTCAAATCATATCTA+GGG 0.535900 tig0028725:+3900 Msa1439180:five_prime_UTR
GATTATGAAGTAAAGACAAA+AGG 0.537647 tig0028725:-5113 None:intergenic
ATCTTTCTATTGAAGTGGCA+AGG 0.545324 tig0028725:+5078 Msa1439180:three_prime_UTR
GCCATGGGGCTACATATCCC+TGG 0.552664 tig0028725:-4456 None:intergenic
TCTTGCTCATCTGTGTCTGA+AGG 0.559484 tig0028725:+4408 Msa1439180:five_prime_UTR
ACAAAATCTCCTTCAAGATG+CGG 0.561408 tig0028725:+3841 Msa1439180:five_prime_UTR
GTGAATCGGAGAAGAAAGGG+AGG 0.561423 tig0028725:-3820 None:intergenic
TAAGGCACGCTAAGGGTGGT+TGG 0.562887 tig0028725:+4726 Msa1439180:CDS
AAAGAGAAGCATGTGAGGGA+AGG 0.591322 tig0028725:-3781 None:intergenic
AACAAACATTACCGATGTGA+AGG 0.591884 tig0028725:-4492 None:intergenic
AGAAGGGGAAAATAGTGTGA+GGG 0.595581 tig0028725:-3751 None:intergenic
GTGGCAAAGAGAAGCATGTG+AGG 0.601561 tig0028725:-3786 None:intergenic
GGGTGGTTGGACAGCAGAAG+AGG 0.605706 tig0028725:+4739 Msa1439180:CDS
AGGGAAGGAAGATAAAGAAG+GGG 0.607295 tig0028725:-3766 None:intergenic
CCTATAAGGCACGCTAAGGG+TGG 0.618742 tig0028725:+4722 Msa1439180:CDS
AGTCCTATAAGGCACGCTAA+GGG 0.620051 tig0028725:+4719 Msa1439180:CDS
AATCGGAGAAGAAAGGGAGG+AGG 0.620302 tig0028725:-3817 None:intergenic
AAGAAGGGGAAAATAGTGTG+AGG 0.621252 tig0028725:-3752 None:intergenic
AAGGGAGGAGGTTCAGATGG+TGG 0.627661 tig0028725:-3805 None:intergenic
ACAAACAAAATCAATAGCAG+AGG 0.629915 tig0028725:-5394 None:intergenic
GTATAATTCAAAAGTCTCCA+GGG 0.641290 tig0028725:+4439 Msa1439180:five_prime_UTR
TCCAGGGATATGTAGCCCCA+TGG 0.641883 tig0028725:+4455 Msa1439180:exon
ATGTCCAGCTCAAAGTGCAG+AGG 0.663657 tig0028725:-5367 None:intergenic
CATCCACACCGCCTTCACAT+CGG 0.670345 tig0028725:+4481 Msa1439180:CDS
TGGCAAAGAGAAGCATGTGA+GGG 0.676512 tig0028725:-3785 None:intergenic
AAACATTACCGATGTGAAGG+CGG 0.687678 tig0028725:-4489 None:intergenic
TTACCGATGTGAAGGCGGTG+TGG 0.693836 tig0028725:-4484 None:intergenic
GCGGTGTGGATGTAGCCATG+GGG 0.784527 tig0028725:-4470 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTAGTAATTAAAGA+TGG - tig0028725:3989-4008 None:intergenic 10.0%
!! AAATAATTAGTAATTAAAGA+TGG - tig0028725:3989-4008 None:intergenic 10.0%
!! AATATAAATGCATTGAATAA+AGG - tig0028725:3940-3959 None:intergenic 15.0%
!!! TTTTTTTATTGGTTTTGTTT+TGG + tig0028725:4149-4168 Msa1439180:intron 15.0%
!!! GATAAAGATTTTGTTTTTTT+GGG + tig0028725:4171-4190 Msa1439180:intron 15.0%
!!! GATTTTTTTTTTTTATACTG+AGG + tig0028725:4275-4294 Msa1439180:intron 15.0%
!! AATATAAATGCATTGAATAA+AGG - tig0028725:3940-3959 None:intergenic 15.0%
!!! TTTTTTTATTGGTTTTGTTT+TGG + tig0028725:4149-4168 Msa1439180:intron 15.0%
!!! GATAAAGATTTTGTTTTTTT+GGG + tig0028725:4171-4190 Msa1439180:intron 15.0%
!!! GATTTTTTTTTTTTATACTG+AGG + tig0028725:4275-4294 Msa1439180:intron 15.0%
!!! GTCAAAAAACGTTTTTTTAT+TGG + tig0028725:4138-4157 Msa1439180:intron 20.0%
!!! GGATAAAGATTTTGTTTTTT+TGG + tig0028725:4170-4189 Msa1439180:intron 20.0%
!!! TTTTTTTGGGAAAAAATTGA+AGG + tig0028725:4184-4203 Msa1439180:intron 20.0%
!!! TTTTTTTTTTATACTGAGGA+TGG + tig0028725:4279-4298 Msa1439180:intron 20.0%
!!! TTTTTAGGGTTTTTTATGTT+TGG + tig0028725:4302-4321 Msa1439180:intron 20.0%
!!! TGTTATGTTATTTACGTTTT+TGG + tig0028725:4511-4530 Msa1439180:intron 20.0%
!!! CATTTTAATTAGAAAGATTG+AGG + tig0028725:5150-5169 Msa1439180:three_prime_UTR 20.0%
!!! GTCAAAAAACGTTTTTTTAT+TGG + tig0028725:4138-4157 Msa1439180:intron 20.0%
!!! GGATAAAGATTTTGTTTTTT+TGG + tig0028725:4170-4189 Msa1439180:intron 20.0%
!!! TTTTTTTGGGAAAAAATTGA+AGG + tig0028725:4184-4203 Msa1439180:intron 20.0%
!!! TTTTTTTTTTATACTGAGGA+TGG + tig0028725:4279-4298 Msa1439180:intron 20.0%
!!! TTTTTAGGGTTTTTTATGTT+TGG + tig0028725:4302-4321 Msa1439180:intron 20.0%
!!! TGTTATGTTATTTACGTTTT+TGG + tig0028725:4511-4530 Msa1439180:intron 20.0%
!!! CATTTTAATTAGAAAGATTG+AGG + tig0028725:5150-5169 Msa1439180:three_prime_UTR 20.0%
! TCTCTAATTGGTCAATTATT+TGG - tig0028725:3568-3587 None:intergenic 25.0%
! TCATCTTCAAATCATATCTA+GGG + tig0028725:3900-3919 Msa1439180:five_prime_UTR 25.0%
!! TTGATTGAGTTTTTGTGTTT+TGG + tig0028725:4092-4111 Msa1439180:intron 25.0%
!!! AGTTTTTGTGTTTTGGATAA+AGG + tig0028725:4099-4118 Msa1439180:intron 25.0%
!!! GTTTTTGTGTTTTGGATAAA+GGG + tig0028725:4100-4119 Msa1439180:intron 25.0%
!!! TTTTTGTGTTTTGGATAAAG+GGG + tig0028725:4101-4120 Msa1439180:intron 25.0%
!!! TGAAGGTTTTGATTTGATTA+TGG + tig0028725:4201-4220 Msa1439180:intron 25.0%
!!! TTTTTATGTTTGGTGATTGA+TGG + tig0028725:4312-4331 Msa1439180:intron 25.0%
!!! TTTTATGTTTGGTGATTGAT+GGG + tig0028725:4313-4332 Msa1439180:intron 25.0%
! ATTGAAGAACACAATAAGAA+CGG - tig0028725:4852-4871 None:intergenic 25.0%
! TGTGTTCTTCAATTTATTGT+AGG + tig0028725:4859-4878 Msa1439180:intron 25.0%
! GATTATGAAGTAAAGACAAA+AGG - tig0028725:5116-5135 None:intergenic 25.0%
! GTAATGTTTCATACATTTCA+AGG - tig0028725:5265-5284 None:intergenic 25.0%
!!! GTGTTTTTTGTTGATTTTGT+TGG + tig0028725:5332-5351 Msa1439180:three_prime_UTR 25.0%
! TCTCTAATTGGTCAATTATT+TGG - tig0028725:3568-3587 None:intergenic 25.0%
! TCATCTTCAAATCATATCTA+GGG + tig0028725:3900-3919 Msa1439180:five_prime_UTR 25.0%
!! TTGATTGAGTTTTTGTGTTT+TGG + tig0028725:4092-4111 Msa1439180:intron 25.0%
!!! AGTTTTTGTGTTTTGGATAA+AGG + tig0028725:4099-4118 Msa1439180:intron 25.0%
!!! GTTTTTGTGTTTTGGATAAA+GGG + tig0028725:4100-4119 Msa1439180:intron 25.0%
!!! TTTTTGTGTTTTGGATAAAG+GGG + tig0028725:4101-4120 Msa1439180:intron 25.0%
!!! TGAAGGTTTTGATTTGATTA+TGG + tig0028725:4201-4220 Msa1439180:intron 25.0%
!!! TTTTTATGTTTGGTGATTGA+TGG + tig0028725:4312-4331 Msa1439180:intron 25.0%
!!! TTTTATGTTTGGTGATTGAT+GGG + tig0028725:4313-4332 Msa1439180:intron 25.0%
! ATTGAAGAACACAATAAGAA+CGG - tig0028725:4852-4871 None:intergenic 25.0%
! TGTGTTCTTCAATTTATTGT+AGG + tig0028725:4859-4878 Msa1439180:intron 25.0%
! GATTATGAAGTAAAGACAAA+AGG - tig0028725:5116-5135 None:intergenic 25.0%
! GTAATGTTTCATACATTTCA+AGG - tig0028725:5265-5284 None:intergenic 25.0%
!!! GTGTTTTTTGTTGATTTTGT+TGG + tig0028725:5332-5351 Msa1439180:three_prime_UTR 25.0%
! TTTTGCTTGTAGTCATGTTT+GGG - tig0028725:3595-3614 None:intergenic 30.0%
! TTTTTGCTTGTAGTCATGTT+TGG - tig0028725:3596-3615 None:intergenic 30.0%
GAAATGTTACATAGGAAATG+TGG - tig0028725:3648-3667 None:intergenic 30.0%
!! TTTTGAAAAGAGAGAAAGGA+AGG - tig0028725:3674-3693 None:intergenic 30.0%
! CCTTTCTCTCTTTTCAAAAT+CGG + tig0028725:3675-3694 Msa1439180:five_prime_UTR 30.0%
! TTGAAGGAGATTTTGTGAAT+CGG - tig0028725:3837-3856 None:intergenic 30.0%
CTCATCTTCAAATCATATCT+AGG + tig0028725:3899-3918 Msa1439180:five_prime_UTR 30.0%
TCGAAGAATTTCAAGATTTC+TGG + tig0028725:4052-4071 Msa1439180:intron 30.0%
!!! TAAAGGGGTTTGATTTGTTT+TGG + tig0028725:4116-4135 Msa1439180:intron 30.0%
!!! TGATTATGGATTTTGAGATC+TGG + tig0028725:4215-4234 Msa1439180:intron 30.0%
!!! GATTATGGATTTTGAGATCT+GGG + tig0028725:4216-4235 Msa1439180:intron 30.0%
TTTGAGTAAGTGTGAAAGTT+TGG + tig0028725:4253-4272 Msa1439180:intron 30.0%
! TTATACTGAGGATGGTTTTT+AGG + tig0028725:4287-4306 Msa1439180:intron 30.0%
! TATACTGAGGATGGTTTTTA+GGG + tig0028725:4288-4307 Msa1439180:intron 30.0%
!! TTTTTAGGGATTGAAGGTTT+TGG + tig0028725:4336-4355 Msa1439180:intron 30.0%
AGTATAATTCAAAAGTCTCC+AGG + tig0028725:4438-4457 Msa1439180:five_prime_UTR 30.0%
GTATAATTCAAAAGTCTCCA+GGG + tig0028725:4439-4458 Msa1439180:five_prime_UTR 30.0%
TTTGGTTGCAGTTGTAATTA+TGG + tig0028725:4529-4548 Msa1439180:intron 30.0%
! TTTTATGCCAAATCTGCTAT+TGG + tig0028725:4588-4607 Msa1439180:intron 30.0%
AATTACACCAATAGCAGATT+TGG - tig0028725:4598-4617 None:intergenic 30.0%
ACCATATGATATGTTTGTAC+AGG + tig0028725:4687-4706 Msa1439180:intron 30.0%
TCCTGTACAAACATATCATA+TGG - tig0028725:4691-4710 None:intergenic 30.0%
ATATGATATGTTTGTACAGG+AGG + tig0028725:4690-4709 Msa1439180:intron 30.0%
AGAGGTTCATATTTGCTTAT+TGG + tig0028725:4757-4776 Msa1439180:intron 30.0%
TATTGTAGGATGAGACATTA+AGG + tig0028725:4873-4892 Msa1439180:intron 30.0%
! TTTTCCCTTAAAAGCTGTAA+CGG - tig0028725:4903-4922 None:intergenic 30.0%
GGAAAACATTGGAAGAAAAT+AGG + tig0028725:4917-4936 Msa1439180:CDS 30.0%
!!! TTGTTGATTTTGTTGGCTAT+TGG + tig0028725:5339-5358 Msa1439180:three_prime_UTR 30.0%
ACAAACAAAATCAATAGCAG+AGG - tig0028725:5397-5416 None:intergenic 30.0%
TTGTAATCATGTCTCATAAC+TGG + tig0028725:5413-5432 Msa1439180:three_prime_UTR 30.0%
! TTTTGCTTGTAGTCATGTTT+GGG - tig0028725:3595-3614 None:intergenic 30.0%
! TTTTTGCTTGTAGTCATGTT+TGG - tig0028725:3596-3615 None:intergenic 30.0%
GAAATGTTACATAGGAAATG+TGG - tig0028725:3648-3667 None:intergenic 30.0%
!! TTTTGAAAAGAGAGAAAGGA+AGG - tig0028725:3674-3693 None:intergenic 30.0%
! CCTTTCTCTCTTTTCAAAAT+CGG + tig0028725:3675-3694 Msa1439180:five_prime_UTR 30.0%
! TTGAAGGAGATTTTGTGAAT+CGG - tig0028725:3837-3856 None:intergenic 30.0%
CTCATCTTCAAATCATATCT+AGG + tig0028725:3899-3918 Msa1439180:five_prime_UTR 30.0%
TCGAAGAATTTCAAGATTTC+TGG + tig0028725:4052-4071 Msa1439180:intron 30.0%
!!! TAAAGGGGTTTGATTTGTTT+TGG + tig0028725:4116-4135 Msa1439180:intron 30.0%
!!! TGATTATGGATTTTGAGATC+TGG + tig0028725:4215-4234 Msa1439180:intron 30.0%
!!! GATTATGGATTTTGAGATCT+GGG + tig0028725:4216-4235 Msa1439180:intron 30.0%
TTTGAGTAAGTGTGAAAGTT+TGG + tig0028725:4253-4272 Msa1439180:intron 30.0%
! TTATACTGAGGATGGTTTTT+AGG + tig0028725:4287-4306 Msa1439180:intron 30.0%
! TATACTGAGGATGGTTTTTA+GGG + tig0028725:4288-4307 Msa1439180:intron 30.0%
!! TTTTTAGGGATTGAAGGTTT+TGG + tig0028725:4336-4355 Msa1439180:intron 30.0%
AGTATAATTCAAAAGTCTCC+AGG + tig0028725:4438-4457 Msa1439180:five_prime_UTR 30.0%
GTATAATTCAAAAGTCTCCA+GGG + tig0028725:4439-4458 Msa1439180:five_prime_UTR 30.0%
TTTGGTTGCAGTTGTAATTA+TGG + tig0028725:4529-4548 Msa1439180:intron 30.0%
! TTTTATGCCAAATCTGCTAT+TGG + tig0028725:4588-4607 Msa1439180:intron 30.0%
AATTACACCAATAGCAGATT+TGG - tig0028725:4598-4617 None:intergenic 30.0%
ACCATATGATATGTTTGTAC+AGG + tig0028725:4687-4706 Msa1439180:intron 30.0%
TCCTGTACAAACATATCATA+TGG - tig0028725:4691-4710 None:intergenic 30.0%
ATATGATATGTTTGTACAGG+AGG + tig0028725:4690-4709 Msa1439180:intron 30.0%
AGAGGTTCATATTTGCTTAT+TGG + tig0028725:4757-4776 Msa1439180:intron 30.0%
TATTGTAGGATGAGACATTA+AGG + tig0028725:4873-4892 Msa1439180:intron 30.0%
! TTTTCCCTTAAAAGCTGTAA+CGG - tig0028725:4903-4922 None:intergenic 30.0%
GGAAAACATTGGAAGAAAAT+AGG + tig0028725:4917-4936 Msa1439180:CDS 30.0%
!!! TTGTTGATTTTGTTGGCTAT+TGG + tig0028725:5339-5358 Msa1439180:three_prime_UTR 30.0%
ACAAACAAAATCAATAGCAG+AGG - tig0028725:5397-5416 None:intergenic 30.0%
TTGTAATCATGTCTCATAAC+TGG + tig0028725:5413-5432 Msa1439180:three_prime_UTR 30.0%
GAAGGAGAGAAATGTTACAT+AGG - tig0028725:3656-3675 None:intergenic 35.0%
!! CCGATTTTGAAAAGAGAGAA+AGG - tig0028725:3678-3697 None:intergenic 35.0%
ATCAGGGTTTGGATTTATGT+TGG - tig0028725:3724-3743 None:intergenic 35.0%
TTTGTGAATCGGAGAAGAAA+GGG - tig0028725:3826-3845 None:intergenic 35.0%
! TTTTGTGAATCGGAGAAGAA+AGG - tig0028725:3827-3846 None:intergenic 35.0%
ACAAAATCTCCTTCAAGATG+CGG + tig0028725:3841-3860 Msa1439180:five_prime_UTR 35.0%
AATTGAAAACCGCATCTTGA+AGG - tig0028725:3853-3872 None:intergenic 35.0%
! TCAAGATGCGGTTTTCAATT+CGG + tig0028725:3853-3872 Msa1439180:five_prime_UTR 35.0%
! TGAAATTCTTCGAAGAGTGT+TGG - tig0028725:4045-4064 None:intergenic 35.0%
!!! TTGGTGATTGATGGGTTTTT+AGG + tig0028725:4321-4340 Msa1439180:intron 35.0%
!!! TGGTGATTGATGGGTTTTTA+GGG + tig0028725:4322-4341 Msa1439180:intron 35.0%
!! GGATATGGATGTTTGTTGTT+TGG + tig0028725:4363-4382 Msa1439180:five_prime_UTR 35.0%
AACAAACATTACCGATGTGA+AGG - tig0028725:4495-4514 None:intergenic 35.0%
!! AATAGTTGCTGATTCACTTC+TGG + tig0028725:4629-4648 Msa1439180:intron 35.0%
!! ATTCAATCCACTTTTCTGGA+AGG - tig0028725:4804-4823 None:intergenic 35.0%
!! TCCAGAAAAGTGGATTGAAT+TGG + tig0028725:4804-4823 Msa1439180:intron 35.0%
!! CCAGAAAAGTGGATTGAATT+GGG + tig0028725:4805-4824 Msa1439180:intron 35.0%
! AATGCCGTTACAGCTTTTAA+GGG + tig0028725:4896-4915 Msa1439180:CDS 35.0%
!! CAGCTTTTAAGGGAAAACAT+TGG + tig0028725:4906-4925 Msa1439180:CDS 35.0%
TTTGTCAAATGCATTAGCTC+AGG - tig0028725:4953-4972 None:intergenic 35.0%
GTCCAATCTTTCTATTGAAG+TGG + tig0028725:5073-5092 Msa1439180:three_prime_UTR 35.0%
! TGCCACTTCAATAGAAAGAT+TGG - tig0028725:5078-5097 None:intergenic 35.0%
ATCTTTCTATTGAAGTGGCA+AGG + tig0028725:5078-5097 Msa1439180:three_prime_UTR 35.0%
GAAGGAGAGAAATGTTACAT+AGG - tig0028725:3656-3675 None:intergenic 35.0%
!! CCGATTTTGAAAAGAGAGAA+AGG - tig0028725:3678-3697 None:intergenic 35.0%
ATCAGGGTTTGGATTTATGT+TGG - tig0028725:3724-3743 None:intergenic 35.0%
TTTGTGAATCGGAGAAGAAA+GGG - tig0028725:3826-3845 None:intergenic 35.0%
! TTTTGTGAATCGGAGAAGAA+AGG - tig0028725:3827-3846 None:intergenic 35.0%
ACAAAATCTCCTTCAAGATG+CGG + tig0028725:3841-3860 Msa1439180:five_prime_UTR 35.0%
AATTGAAAACCGCATCTTGA+AGG - tig0028725:3853-3872 None:intergenic 35.0%
! TCAAGATGCGGTTTTCAATT+CGG + tig0028725:3853-3872 Msa1439180:five_prime_UTR 35.0%
! TGAAATTCTTCGAAGAGTGT+TGG - tig0028725:4045-4064 None:intergenic 35.0%
!!! TTGGTGATTGATGGGTTTTT+AGG + tig0028725:4321-4340 Msa1439180:intron 35.0%
!!! TGGTGATTGATGGGTTTTTA+GGG + tig0028725:4322-4341 Msa1439180:intron 35.0%
!! GGATATGGATGTTTGTTGTT+TGG + tig0028725:4363-4382 Msa1439180:five_prime_UTR 35.0%
AACAAACATTACCGATGTGA+AGG - tig0028725:4495-4514 None:intergenic 35.0%
!! AATAGTTGCTGATTCACTTC+TGG + tig0028725:4629-4648 Msa1439180:intron 35.0%
!! ATTCAATCCACTTTTCTGGA+AGG - tig0028725:4804-4823 None:intergenic 35.0%
!! TCCAGAAAAGTGGATTGAAT+TGG + tig0028725:4804-4823 Msa1439180:intron 35.0%
!! CCAGAAAAGTGGATTGAATT+GGG + tig0028725:4805-4824 Msa1439180:intron 35.0%
! AATGCCGTTACAGCTTTTAA+GGG + tig0028725:4896-4915 Msa1439180:CDS 35.0%
!! CAGCTTTTAAGGGAAAACAT+TGG + tig0028725:4906-4925 Msa1439180:CDS 35.0%
TTTGTCAAATGCATTAGCTC+AGG - tig0028725:4953-4972 None:intergenic 35.0%
GTCCAATCTTTCTATTGAAG+TGG + tig0028725:5073-5092 Msa1439180:three_prime_UTR 35.0%
! TGCCACTTCAATAGAAAGAT+TGG - tig0028725:5078-5097 None:intergenic 35.0%
ATCTTTCTATTGAAGTGGCA+AGG + tig0028725:5078-5097 Msa1439180:three_prime_UTR 35.0%
TGTTTGGGCTTCTCTCTAAT+TGG - tig0028725:3580-3599 None:intergenic 40.0%
! GTTTGGATTTATGTTGGTGG+AGG - tig0028725:3718-3737 None:intergenic 40.0%
AGGGTTTGGATTTATGTTGG+TGG - tig0028725:3721-3740 None:intergenic 40.0%
AGAAGGGGAAAATAGTGTGA+GGG - tig0028725:3754-3773 None:intergenic 40.0%
AAGAAGGGGAAAATAGTGTG+AGG - tig0028725:3755-3774 None:intergenic 40.0%
AGGGAAGGAAGATAAAGAAG+GGG - tig0028725:3769-3788 None:intergenic 40.0%
GAGGGAAGGAAGATAAAGAA+GGG - tig0028725:3770-3789 None:intergenic 40.0%
TGAGGGAAGGAAGATAAAGA+AGG - tig0028725:3771-3790 None:intergenic 40.0%
!! GATGGGTTTTTAGGGATTGA+AGG + tig0028725:4330-4349 Msa1439180:intron 40.0%
!! GAAGGTTTTGGTGAAGGATA+TGG + tig0028725:4348-4367 Msa1439180:five_prime_UTR 40.0%
AAACATTACCGATGTGAAGG+CGG - tig0028725:4492-4511 None:intergenic 40.0%
! TTTTCCTCCTTCCAGAAAAG+TGG + tig0028725:4794-4813 Msa1439180:intron 40.0%
!! CCCAATTCAATCCACTTTTC+TGG - tig0028725:4808-4827 None:intergenic 40.0%
! GAATGCCGTTACAGCTTTTA+AGG + tig0028725:4895-4914 Msa1439180:CDS 40.0%
ATTGAAGTGGCAAGGATGAT+TGG + tig0028725:5086-5105 Msa1439180:three_prime_UTR 40.0%
TGTTTGGGCTTCTCTCTAAT+TGG - tig0028725:3580-3599 None:intergenic 40.0%
! GTTTGGATTTATGTTGGTGG+AGG - tig0028725:3718-3737 None:intergenic 40.0%
AGGGTTTGGATTTATGTTGG+TGG - tig0028725:3721-3740 None:intergenic 40.0%
AGAAGGGGAAAATAGTGTGA+GGG - tig0028725:3754-3773 None:intergenic 40.0%
AAGAAGGGGAAAATAGTGTG+AGG - tig0028725:3755-3774 None:intergenic 40.0%
AGGGAAGGAAGATAAAGAAG+GGG - tig0028725:3769-3788 None:intergenic 40.0%
GAGGGAAGGAAGATAAAGAA+GGG - tig0028725:3770-3789 None:intergenic 40.0%
TGAGGGAAGGAAGATAAAGA+AGG - tig0028725:3771-3790 None:intergenic 40.0%
!! GATGGGTTTTTAGGGATTGA+AGG + tig0028725:4330-4349 Msa1439180:intron 40.0%
!! GAAGGTTTTGGTGAAGGATA+TGG + tig0028725:4348-4367 Msa1439180:five_prime_UTR 40.0%
AAACATTACCGATGTGAAGG+CGG - tig0028725:4492-4511 None:intergenic 40.0%
! TTTTCCTCCTTCCAGAAAAG+TGG + tig0028725:4794-4813 Msa1439180:intron 40.0%
!! CCCAATTCAATCCACTTTTC+TGG - tig0028725:4808-4827 None:intergenic 40.0%
! GAATGCCGTTACAGCTTTTA+AGG + tig0028725:4895-4914 Msa1439180:CDS 40.0%
ATTGAAGTGGCAAGGATGAT+TGG + tig0028725:5086-5105 Msa1439180:three_prime_UTR 40.0%
! TGGATTTATGTTGGTGGAGG+TGG - tig0028725:3715-3734 None:intergenic 45.0%
AGGGAGAAGATATCAGGGTT+TGG - tig0028725:3735-3754 None:intergenic 45.0%
GTGTGAGGGAGAAGATATCA+GGG - tig0028725:3740-3759 None:intergenic 45.0%
AGTGTGAGGGAGAAGATATC+AGG - tig0028725:3741-3760 None:intergenic 45.0%
! AAAGAGAAGCATGTGAGGGA+AGG - tig0028725:3784-3803 None:intergenic 45.0%
! TGGCAAAGAGAAGCATGTGA+GGG - tig0028725:3788-3807 None:intergenic 45.0%
!! GGGATTGAAGGTTTTGGTGA+AGG + tig0028725:4342-4361 Msa1439180:intron 45.0%
TCTTGCTCATCTGTGTCTGA+AGG + tig0028725:4408-4427 Msa1439180:five_prime_UTR 45.0%
GGAGGAGAACAAGTCCTATA+AGG + tig0028725:4708-4727 Msa1439180:intron 45.0%
AAGTCCTATAAGGCACGCTA+AGG + tig0028725:4718-4737 Msa1439180:CDS 45.0%
AGTCCTATAAGGCACGCTAA+GGG + tig0028725:4719-4738 Msa1439180:CDS 45.0%
!! CAATCCACTTTTCTGGAAGG+AGG - tig0028725:4801-4820 None:intergenic 45.0%
! TGGATTTATGTTGGTGGAGG+TGG - tig0028725:3715-3734 None:intergenic 45.0%
AGGGAGAAGATATCAGGGTT+TGG - tig0028725:3735-3754 None:intergenic 45.0%
GTGTGAGGGAGAAGATATCA+GGG - tig0028725:3740-3759 None:intergenic 45.0%
AGTGTGAGGGAGAAGATATC+AGG - tig0028725:3741-3760 None:intergenic 45.0%
! AAAGAGAAGCATGTGAGGGA+AGG - tig0028725:3784-3803 None:intergenic 45.0%
! TGGCAAAGAGAAGCATGTGA+GGG - tig0028725:3788-3807 None:intergenic 45.0%
!! GGGATTGAAGGTTTTGGTGA+AGG + tig0028725:4342-4361 Msa1439180:intron 45.0%
TCTTGCTCATCTGTGTCTGA+AGG + tig0028725:4408-4427 Msa1439180:five_prime_UTR 45.0%
GGAGGAGAACAAGTCCTATA+AGG + tig0028725:4708-4727 Msa1439180:intron 45.0%
AAGTCCTATAAGGCACGCTA+AGG + tig0028725:4718-4737 Msa1439180:CDS 45.0%
AGTCCTATAAGGCACGCTAA+GGG + tig0028725:4719-4738 Msa1439180:CDS 45.0%
!! CAATCCACTTTTCTGGAAGG+AGG - tig0028725:4801-4820 None:intergenic 45.0%
! GTGGCAAAGAGAAGCATGTG+AGG - tig0028725:3789-3808 None:intergenic 50.0%
AGAAAGGGAGGAGGTTCAGA+TGG - tig0028725:3811-3830 None:intergenic 50.0%
AATCGGAGAAGAAAGGGAGG+AGG - tig0028725:3820-3839 None:intergenic 50.0%
GTGAATCGGAGAAGAAAGGG+AGG - tig0028725:3823-3842 None:intergenic 50.0%
TTTGCCTCTGCACTTTGAGC+TGG + tig0028725:5363-5382 Msa1439180:three_prime_UTR 50.0%
ATGTCCAGCTCAAAGTGCAG+AGG - tig0028725:5370-5389 None:intergenic 50.0%
! GTGGCAAAGAGAAGCATGTG+AGG - tig0028725:3789-3808 None:intergenic 50.0%
AGAAAGGGAGGAGGTTCAGA+TGG - tig0028725:3811-3830 None:intergenic 50.0%
AATCGGAGAAGAAAGGGAGG+AGG - tig0028725:3820-3839 None:intergenic 50.0%
GTGAATCGGAGAAGAAAGGG+AGG - tig0028725:3823-3842 None:intergenic 50.0%
TTTGCCTCTGCACTTTGAGC+TGG + tig0028725:5363-5382 Msa1439180:three_prime_UTR 50.0%
ATGTCCAGCTCAAAGTGCAG+AGG - tig0028725:5370-5389 None:intergenic 50.0%
AAGGGAGGAGGTTCAGATGG+TGG - tig0028725:3808-3827 None:intergenic 55.0%
TCCAGGGATATGTAGCCCCA+TGG + tig0028725:4455-4474 Msa1439180:exon 55.0%
CATCCACACCGCCTTCACAT+CGG + tig0028725:4481-4500 Msa1439180:CDS 55.0%
TTACCGATGTGAAGGCGGTG+TGG - tig0028725:4487-4506 None:intergenic 55.0%
CCACCCTTAGCGTGCCTTAT+AGG - tig0028725:4725-4744 None:intergenic 55.0%
CCTATAAGGCACGCTAAGGG+TGG + tig0028725:4722-4741 Msa1439180:CDS 55.0%
TAAGGCACGCTAAGGGTGGT+TGG + tig0028725:4726-4745 Msa1439180:CDS 55.0%
AAGGGAGGAGGTTCAGATGG+TGG - tig0028725:3808-3827 None:intergenic 55.0%
TCCAGGGATATGTAGCCCCA+TGG + tig0028725:4455-4474 Msa1439180:exon 55.0%
CATCCACACCGCCTTCACAT+CGG + tig0028725:4481-4500 Msa1439180:CDS 55.0%
TTACCGATGTGAAGGCGGTG+TGG - tig0028725:4487-4506 None:intergenic 55.0%
CCACCCTTAGCGTGCCTTAT+AGG - tig0028725:4725-4744 None:intergenic 55.0%
CCTATAAGGCACGCTAAGGG+TGG + tig0028725:4722-4741 Msa1439180:CDS 55.0%
TAAGGCACGCTAAGGGTGGT+TGG + tig0028725:4726-4745 Msa1439180:CDS 55.0%
GCCATGGGGCTACATATCCC+TGG - tig0028725:4459-4478 None:intergenic 60.0%
GCGGTGTGGATGTAGCCATG+GGG - tig0028725:4473-4492 None:intergenic 60.0%
GGCGGTGTGGATGTAGCCAT+GGG - tig0028725:4474-4493 None:intergenic 60.0%
AGGCGGTGTGGATGTAGCCA+TGG - tig0028725:4475-4494 None:intergenic 60.0%
GGGTGGTTGGACAGCAGAAG+AGG + tig0028725:4739-4758 Msa1439180:CDS 60.0%
GCCATGGGGCTACATATCCC+TGG - tig0028725:4459-4478 None:intergenic 60.0%
GCGGTGTGGATGTAGCCATG+GGG - tig0028725:4473-4492 None:intergenic 60.0%
GGCGGTGTGGATGTAGCCAT+GGG - tig0028725:4474-4493 None:intergenic 60.0%
AGGCGGTGTGGATGTAGCCA+TGG - tig0028725:4475-4494 None:intergenic 60.0%
GGGTGGTTGGACAGCAGAAG+AGG + tig0028725:4739-4758 Msa1439180:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0028725 gene 3557 5485 3557 ID=Msa1439180;Name=Msa1439180
tig0028725 mRNA 3557 5485 3557 ID=Msa1439180-mRNA-1;Parent=Msa1439180;Name=Msa1439180-mRNA-1;_AED=0.32;_eAED=0.32;_QI=496|1|1|1|0.66|0.5|4|509|58
tig0028725 exon 3557 3921 3557 ID=Msa1439180-mRNA-1:exon:11663;Parent=Msa1439180-mRNA-1
tig0028725 exon 4343 4502 4343 ID=Msa1439180-mRNA-1:exon:11664;Parent=Msa1439180-mRNA-1
tig0028725 exon 4709 4760 4709 ID=Msa1439180-mRNA-1:exon:11665;Parent=Msa1439180-mRNA-1
tig0028725 exon 4881 5485 4881 ID=Msa1439180-mRNA-1:exon:11666;Parent=Msa1439180-mRNA-1
tig0028725 five_prime_UTR 3557 3921 3557 ID=Msa1439180-mRNA-1:five_prime_utr;Parent=Msa1439180-mRNA-1
tig0028725 five_prime_UTR 4343 4473 4343 ID=Msa1439180-mRNA-1:five_prime_utr;Parent=Msa1439180-mRNA-1
tig0028725 CDS 4474 4502 4474 ID=Msa1439180-mRNA-1:cds;Parent=Msa1439180-mRNA-1
tig0028725 CDS 4709 4760 4709 ID=Msa1439180-mRNA-1:cds;Parent=Msa1439180-mRNA-1
tig0028725 CDS 4881 4976 4881 ID=Msa1439180-mRNA-1:cds;Parent=Msa1439180-mRNA-1
tig0028725 three_prime_UTR 4977 5485 4977 ID=Msa1439180-mRNA-1:three_prime_utr;Parent=Msa1439180-mRNA-1
Gene Sequence

>Msa1439180

ATGGCTACATCCACACCGCCTTCACATCGGAGGAGAACAAGTCCTATAAGGCACGCTAAGGGTGGTTGGACAGCAGAAGAGGATGAGACATTAAGGAATGCCGTTACAGCTTTTAAGGGAAAACATTGGAAGAAAATAGGTAACTATTTGCCTGAGCTAATGCATTTGACAAATTAA
Protein sequence

>Msa1439180

MATSTPPSHRRRTSPIRHAKGGWTAEEDETLRNAVTAFKGKHWKKIGNYLPELMHLTN*