AlfalfaGEDB | Msa1002900

M. sativa cultivar zhongmu-4 / Msa1002900

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0008150 GO:0008285 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032465 GO:0032502 GO:0042127 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046677 GO:0047484 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051302 GO:0051726 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0140110 GO:1901000 GO:1901002 GO:1901181 GO:1901363 GO:1901700 GO:1902584 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000070 GO:2000112 GO:2000113 GO:2001023 GO:2001024 GO:2001038 GO:2001039 GO:2001141
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1002900	XP_003623113.1	78.431	51	11	0	2	52	43	93	2.88e-18	87.4

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1002900	sp\|Q6R032\|MB3R5_ARATH	65.116	43	15	0	10	52	65	107	2.73e-13	65.1

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1002900	G7L4J7	78.431	51	11	0	2	52	43	93	1.37e-18	87.4

TFs/TRs:

Gene ID	Type	Classification
Msa1002900	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0622420	Msa1002900	0.894674	1.974758e-75	-8.615850e-47
Msa0991170	Msa1002900	0.881820	1.747153e-70	-8.615850e-47
Msa1002900	Msa1218700	0.948322	1.187057e-106	-8.615850e-47
Msa1002900	Msa1419060	0.910408	1.936311e-82	-8.615850e-47
Msa1002900	Msa1439180	0.831805	1.355443e-55	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1002900	MtrunA17_Chr7g0238631	78.431	51	11	0	2	52	43	93	2.64e-22	87.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1002900	AT2G13960.1	65.116	43	15	0	10	52	65	107	2.13e-15	65.5
Msa1002900	AT2G13960.2	65.116	43	15	0	10	52	30	72	2.75e-15	64.3
Msa1002900	AT5G02320.1	65.116	43	15	0	10	52	65	107	2.78e-14	65.1
Msa1002900	AT5G02320.2	65.116	43	15	0	10	52	65	107	2.78e-14	65.1
Msa1002900	AT5G02320.3	65.116	43	15	0	10	52	5	47	3.82e-14	64.7
Msa1002900	AT3G09370.2	61.224	49	16	1	7	52	67	115	1.13e-13	63.5
Msa1002900	AT3G09370.1	56.604	53	20	1	7	56	62	114	1.14e-13	63.5
Msa1002900	AT3G09370.3	61.224	49	16	1	7	52	75	123	1.15e-13	63.5

Find 61 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTCTAATTGGTCAATTATT+TGG	0.166929	6_4:+89368604	None:intergenic
CCACCCTTAGCGTGCCTTAT+AGG	0.202151	6_4:+89367446	None:intergenic
TATTGTAGGATGAGACATTA+AGG	0.293653	6_4:-89367295	Msa1002900:intron
TGGATTTATGTTGGTGGAGG+TGG	0.309935	6_4:+89368457	None:intergenic
GGAAAACATTGGAAGAAAAT+AGG	0.311225	6_4:-89367251	Msa1002900:CDS
TGTTTGGGCTTCTCTCTAAT+TGG	0.328403	6_4:+89368592	None:intergenic
CTCATCTTCAAATCATATCT+AGG	0.331174	6_4:-89368270	Msa1002900:five_prime_UTR
AGTGTGAGGGAGAAGATATC+AGG	0.349806	6_4:+89368431	None:intergenic
TTGTAATCATGTCTCATAAC+TGG	0.356897	6_4:-89366755	Msa1002900:three_prime_UTR
TGCCACTTCAATAGAAAGAT+TGG	0.375619	6_4:+89367093	None:intergenic
GGATATGGATGTTTGTTGTT+TGG	0.389621	6_4:-89367805	Msa1002900:five_prime_UTR
ATTGAAGTGGCAAGGATGAT+TGG	0.406073	6_4:-89367082	Msa1002900:three_prime_UTR
GGAGGAGAACAAGTCCTATA+AGG	0.409948	6_4:-89367460	Msa1002900:CDS
TTTGCCTCTGCACTTTGAGC+TGG	0.415507	6_4:-89366805	Msa1002900:three_prime_UTR
GTAATGTTTCATACATTTCA+AGG	0.423379	6_4:+89366906	None:intergenic
AGGGAGAAGATATCAGGGTT+TGG	0.428253	6_4:+89368437	None:intergenic
AGGGTTTGGATTTATGTTGG+TGG	0.431049	6_4:+89368451	None:intergenic
AATTGAAAACCGCATCTTGA+AGG	0.449911	6_4:+89368319	None:intergenic
AGTATAATTCAAAAGTCTCC+AGG	0.458256	6_4:-89367730	Msa1002900:five_prime_UTR
TTTGTCAAATGCATTAGCTC+AGG	0.463353	6_4:+89367218	None:intergenic
ATCAGGGTTTGGATTTATGT+TGG	0.464167	6_4:+89368448	None:intergenic
TTTGTGAATCGGAGAAGAAA+GGG	0.464883	6_4:+89368346	None:intergenic
GTCCAATCTTTCTATTGAAG+TGG	0.468597	6_4:-89367095	Msa1002900:three_prime_UTR
AGAAAGGGAGGAGGTTCAGA+TGG	0.474386	6_4:+89368361	None:intergenic
AGGCGGTGTGGATGTAGCCA+TGG	0.485223	6_4:+89367696	None:intergenic
GTTTGGATTTATGTTGGTGG+AGG	0.500329	6_4:+89368454	None:intergenic
GGCGGTGTGGATGTAGCCAT+GGG	0.512625	6_4:+89367697	None:intergenic
GAAGGAGAGAAATGTTACAT+AGG	0.517797	6_4:+89368516	None:intergenic
GAGGGAAGGAAGATAAAGAA+GGG	0.520886	6_4:+89368402	None:intergenic
GAAATGTTACATAGGAAATG+TGG	0.521689	6_4:+89368524	None:intergenic
GTGTGAGGGAGAAGATATCA+GGG	0.524168	6_4:+89368432	None:intergenic
TGAGGGAAGGAAGATAAAGA+AGG	0.525432	6_4:+89368401	None:intergenic
AAGTCCTATAAGGCACGCTA+AGG	0.528953	6_4:-89367450	Msa1002900:CDS
TCATCTTCAAATCATATCTA+GGG	0.535900	6_4:-89368269	Msa1002900:intron
GATTATGAAGTAAAGACAAA+AGG	0.537647	6_4:+89367055	None:intergenic
ATCTTTCTATTGAAGTGGCA+AGG	0.545324	6_4:-89367090	Msa1002900:three_prime_UTR
GCCATGGGGCTACATATCCC+TGG	0.552664	6_4:+89367712	None:intergenic
TCTTGCTCATCTGTGTCTGA+AGG	0.559484	6_4:-89367760	Msa1002900:five_prime_UTR
ACAAAATCTCCTTCAAGATG+CGG	0.561408	6_4:-89368328	Msa1002900:five_prime_UTR
GTGAATCGGAGAAGAAAGGG+AGG	0.561423	6_4:+89368349	None:intergenic
TAAGGCACGCTAAGGGTGGT+TGG	0.562887	6_4:-89367442	Msa1002900:CDS
AAAGAGAAGCATGTGAGGGA+AGG	0.591322	6_4:+89368388	None:intergenic
AACAAACATTACCGATGTGA+AGG	0.591884	6_4:+89367676	None:intergenic
AGAAGGGGAAAATAGTGTGA+GGG	0.595581	6_4:+89368418	None:intergenic
GTGGCAAAGAGAAGCATGTG+AGG	0.601561	6_4:+89368383	None:intergenic
GGGTGGTTGGACAGCAGAAG+AGG	0.605706	6_4:-89367429	Msa1002900:intron
AGGGAAGGAAGATAAAGAAG+GGG	0.607295	6_4:+89368403	None:intergenic
CCTATAAGGCACGCTAAGGG+TGG	0.618742	6_4:-89367446	Msa1002900:CDS
AGTCCTATAAGGCACGCTAA+GGG	0.620051	6_4:-89367449	Msa1002900:CDS
AATCGGAGAAGAAAGGGAGG+AGG	0.620302	6_4:+89368352	None:intergenic
AAGAAGGGGAAAATAGTGTG+AGG	0.621252	6_4:+89368417	None:intergenic
AAGGGAGGAGGTTCAGATGG+TGG	0.627661	6_4:+89368364	None:intergenic
ACAAACAAAATCAATAGCAG+AGG	0.629915	6_4:+89366774	None:intergenic
GTATAATTCAAAAGTCTCCA+GGG	0.641290	6_4:-89367729	Msa1002900:five_prime_UTR
TCCAGGGATATGTAGCCCCA+TGG	0.641883	6_4:-89367713	Msa1002900:exon
ATGTCCAGCTCAAAGTGCAG+AGG	0.663657	6_4:+89366801	None:intergenic
CATCCACACCGCCTTCACAT+CGG	0.670345	6_4:-89367687	Msa1002900:intron
TGGCAAAGAGAAGCATGTGA+GGG	0.676512	6_4:+89368384	None:intergenic
AAACATTACCGATGTGAAGG+CGG	0.687678	6_4:+89367679	None:intergenic
TTACCGATGTGAAGGCGGTG+TGG	0.693836	6_4:+89367684	None:intergenic
GCGGTGTGGATGTAGCCATG+GGG	0.784527	6_4:+89367698	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATAATTAGTAATTAAAGA+TGG	+	chr6_4:89367138-89367157	None:intergenic	10.0%
!!!	ATTTTTTTTTTTTTATACTG+AGG	-	chr6_4:89367424-89367443	Msa1002900:intron	10.0%
!!	AAATAATTAGTAATTAAAGA+TGG	+	chr6_4:89367138-89367157	None:intergenic	10.0%
!!!	ATTTTTTTTTTTTTATACTG+AGG	-	chr6_4:89367424-89367443	Msa1002900:intron	10.0%
!!	AATATAAATGCATTGAATAA+AGG	+	chr6_4:89367088-89367107	None:intergenic	15.0%
!!!	TTTTTTTATTGGTTTTGTTT+TGG	-	chr6_4:89367298-89367317	Msa1002900:intron	15.0%
!!!	GATAAAGATTTTGTTTTTTT+GGG	-	chr6_4:89367320-89367339	Msa1002900:intron	15.0%
!!	AATATAAATGCATTGAATAA+AGG	+	chr6_4:89367088-89367107	None:intergenic	15.0%
!!!	TTTTTTTATTGGTTTTGTTT+TGG	-	chr6_4:89367298-89367317	Msa1002900:intron	15.0%
!!!	GATAAAGATTTTGTTTTTTT+GGG	-	chr6_4:89367320-89367339	Msa1002900:intron	15.0%
!!!	GTCAAAAAACGTTTTTTTAT+TGG	-	chr6_4:89367287-89367306	Msa1002900:CDS	20.0%
!!!	GGATAAAGATTTTGTTTTTT+TGG	-	chr6_4:89367319-89367338	Msa1002900:intron	20.0%
!!!	TTTTTTTGGGAAAAAATTGA+AGG	-	chr6_4:89367333-89367352	Msa1002900:intron	20.0%
!!!	TTTTTTTTTTATACTGAGGA+TGG	-	chr6_4:89367428-89367447	Msa1002900:intron	20.0%
!!!	TTTTTAGGGTTTTTTATGTT+TGG	-	chr6_4:89367451-89367470	Msa1002900:CDS	20.0%
!!!	TGTTATGTTATTTACGTTTT+TGG	-	chr6_4:89367660-89367679	Msa1002900:intron	20.0%
!!!	CATTTTAATTAGAAAGATTG+AGG	-	chr6_4:89368299-89368318	Msa1002900:five_prime_UTR	20.0%
!!!	GTCAAAAAACGTTTTTTTAT+TGG	-	chr6_4:89367287-89367306	Msa1002900:CDS	20.0%
!!!	GGATAAAGATTTTGTTTTTT+TGG	-	chr6_4:89367319-89367338	Msa1002900:intron	20.0%
!!!	TTTTTTTGGGAAAAAATTGA+AGG	-	chr6_4:89367333-89367352	Msa1002900:intron	20.0%
!!!	TTTTTTTTTTATACTGAGGA+TGG	-	chr6_4:89367428-89367447	Msa1002900:intron	20.0%
!!!	TTTTTAGGGTTTTTTATGTT+TGG	-	chr6_4:89367451-89367470	Msa1002900:CDS	20.0%
!!!	TGTTATGTTATTTACGTTTT+TGG	-	chr6_4:89367660-89367679	Msa1002900:intron	20.0%
!!!	CATTTTAATTAGAAAGATTG+AGG	-	chr6_4:89368299-89368318	Msa1002900:five_prime_UTR	20.0%
!	TCTCTAATTGGTCAATTATT+TGG	+	chr6_4:89366716-89366735	None:intergenic	25.0%
!	TCATCTTCAAATCATATCTA+GGG	-	chr6_4:89367048-89367067	Msa1002900:three_prime_UTR	25.0%
!!	TTGATTGAGTTTTTGTGTTT+TGG	-	chr6_4:89367241-89367260	Msa1002900:CDS	25.0%
!!!	AGTTTTTGTGTTTTGGATAA+AGG	-	chr6_4:89367248-89367267	Msa1002900:CDS	25.0%
!!!	GTTTTTGTGTTTTGGATAAA+GGG	-	chr6_4:89367249-89367268	Msa1002900:CDS	25.0%
!!!	TTTTTGTGTTTTGGATAAAG+GGG	-	chr6_4:89367250-89367269	Msa1002900:CDS	25.0%
!!!	TGAAGGTTTTGATTTGATTA+TGG	-	chr6_4:89367350-89367369	Msa1002900:intron	25.0%
!!!	TTTTTATGTTTGGTGATTGA+TGG	-	chr6_4:89367461-89367480	Msa1002900:CDS	25.0%
!!!	TTTTATGTTTGGTGATTGAT+GGG	-	chr6_4:89367462-89367481	Msa1002900:CDS	25.0%
!	ATTGAAGAACACAATAAGAA+CGG	+	chr6_4:89368001-89368020	None:intergenic	25.0%
!	TGTGTTCTTCAATTTATTGT+AGG	-	chr6_4:89368008-89368027	Msa1002900:intron	25.0%
!	GATTATGAAGTAAAGACAAA+AGG	+	chr6_4:89368265-89368284	None:intergenic	25.0%
!	GTAATGTTTCATACATTTCA+AGG	+	chr6_4:89368414-89368433	None:intergenic	25.0%
!!!	GTGTTTTTTGTTGATTTTGT+TGG	-	chr6_4:89368481-89368500	Msa1002900:five_prime_UTR	25.0%
!	TCTCTAATTGGTCAATTATT+TGG	+	chr6_4:89366716-89366735	None:intergenic	25.0%
!	TCATCTTCAAATCATATCTA+GGG	-	chr6_4:89367048-89367067	Msa1002900:three_prime_UTR	25.0%
!!	TTGATTGAGTTTTTGTGTTT+TGG	-	chr6_4:89367241-89367260	Msa1002900:CDS	25.0%
!!!	AGTTTTTGTGTTTTGGATAA+AGG	-	chr6_4:89367248-89367267	Msa1002900:CDS	25.0%
!!!	GTTTTTGTGTTTTGGATAAA+GGG	-	chr6_4:89367249-89367268	Msa1002900:CDS	25.0%
!!!	TTTTTGTGTTTTGGATAAAG+GGG	-	chr6_4:89367250-89367269	Msa1002900:CDS	25.0%
!!!	TGAAGGTTTTGATTTGATTA+TGG	-	chr6_4:89367350-89367369	Msa1002900:intron	25.0%
!!!	TTTTTATGTTTGGTGATTGA+TGG	-	chr6_4:89367461-89367480	Msa1002900:CDS	25.0%
!!!	TTTTATGTTTGGTGATTGAT+GGG	-	chr6_4:89367462-89367481	Msa1002900:CDS	25.0%
!	ATTGAAGAACACAATAAGAA+CGG	+	chr6_4:89368001-89368020	None:intergenic	25.0%
!	TGTGTTCTTCAATTTATTGT+AGG	-	chr6_4:89368008-89368027	Msa1002900:intron	25.0%
!	GATTATGAAGTAAAGACAAA+AGG	+	chr6_4:89368265-89368284	None:intergenic	25.0%
!	GTAATGTTTCATACATTTCA+AGG	+	chr6_4:89368414-89368433	None:intergenic	25.0%
!!!	GTGTTTTTTGTTGATTTTGT+TGG	-	chr6_4:89368481-89368500	Msa1002900:five_prime_UTR	25.0%
!	TTTTGCTTGTAGTCATGTTT+GGG	+	chr6_4:89366743-89366762	None:intergenic	30.0%
!	TTTTTGCTTGTAGTCATGTT+TGG	+	chr6_4:89366744-89366763	None:intergenic	30.0%
	GAAATGTTACATAGGAAATG+TGG	+	chr6_4:89366796-89366815	None:intergenic	30.0%
!!	TTTTGAAAAGAGAGAAAGGA+AGG	+	chr6_4:89366822-89366841	None:intergenic	30.0%
!	CCTTTCTCTCTTTTCAAAAT+CGG	-	chr6_4:89366823-89366842	Msa1002900:three_prime_UTR	30.0%
!	TTGAAGGAGATTTTGTGAAT+CGG	+	chr6_4:89366985-89367004	None:intergenic	30.0%
	CTCATCTTCAAATCATATCT+AGG	-	chr6_4:89367047-89367066	Msa1002900:three_prime_UTR	30.0%
	TCGAAGAATTTCAAGATTTC+TGG	-	chr6_4:89367201-89367220	Msa1002900:exon	30.0%
!!!	TAAAGGGGTTTGATTTGTTT+TGG	-	chr6_4:89367265-89367284	Msa1002900:CDS	30.0%
!!!	TGATTATGGATTTTGAGATC+TGG	-	chr6_4:89367364-89367383	Msa1002900:intron	30.0%
!!!	GATTATGGATTTTGAGATCT+GGG	-	chr6_4:89367365-89367384	Msa1002900:intron	30.0%
!	TGAGTAAAAGTGTGAAAGTT+TGG	-	chr6_4:89367401-89367420	Msa1002900:intron	30.0%
!	TTATACTGAGGATGGTTTTT+AGG	-	chr6_4:89367436-89367455	Msa1002900:CDS	30.0%
!	TATACTGAGGATGGTTTTTA+GGG	-	chr6_4:89367437-89367456	Msa1002900:CDS	30.0%
!!	TTTTTAGGGATTGAAGGTTT+TGG	-	chr6_4:89367485-89367504	Msa1002900:intron	30.0%
	AGTATAATTCAAAAGTCTCC+AGG	-	chr6_4:89367587-89367606	Msa1002900:intron	30.0%
	GTATAATTCAAAAGTCTCCA+GGG	-	chr6_4:89367588-89367607	Msa1002900:intron	30.0%
	TTTGGTTGCAGTTGTAATTA+TGG	-	chr6_4:89367678-89367697	Msa1002900:intron	30.0%
!	TTTTATGCCAAATCTGCTAT+TGG	-	chr6_4:89367737-89367756	Msa1002900:five_prime_UTR	30.0%
	AATTACACCAATAGCAGATT+TGG	+	chr6_4:89367747-89367766	None:intergenic	30.0%
	ACCATATGATATGTTTGTAC+AGG	-	chr6_4:89367836-89367855	Msa1002900:intron	30.0%
	TCCTGTACAAACATATCATA+TGG	+	chr6_4:89367840-89367859	None:intergenic	30.0%
	ATATGATATGTTTGTACAGG+AGG	-	chr6_4:89367839-89367858	Msa1002900:intron	30.0%
	AGAGGTTCATATTTGCTTAT+TGG	-	chr6_4:89367906-89367925	Msa1002900:intron	30.0%
	TATTGTAGGATGAGACATTA+AGG	-	chr6_4:89368022-89368041	Msa1002900:intron	30.0%
!	TTTTCCCTTAAAAGCTGTAA+CGG	+	chr6_4:89368052-89368071	None:intergenic	30.0%
	GGAAAACATTGGAAGAAAAT+AGG	-	chr6_4:89368066-89368085	Msa1002900:intron	30.0%
!!!	TTGTTGATTTTGTTGGCTAT+TGG	-	chr6_4:89368488-89368507	Msa1002900:five_prime_UTR	30.0%
	ACAAACAAAATCAATAGCAG+AGG	+	chr6_4:89368546-89368565	None:intergenic	30.0%
	TTGTAATCATGTCTCATAAC+TGG	-	chr6_4:89368562-89368581	Msa1002900:five_prime_UTR	30.0%
!	TTTTGCTTGTAGTCATGTTT+GGG	+	chr6_4:89366743-89366762	None:intergenic	30.0%
!	TTTTTGCTTGTAGTCATGTT+TGG	+	chr6_4:89366744-89366763	None:intergenic	30.0%
	GAAATGTTACATAGGAAATG+TGG	+	chr6_4:89366796-89366815	None:intergenic	30.0%
!!	TTTTGAAAAGAGAGAAAGGA+AGG	+	chr6_4:89366822-89366841	None:intergenic	30.0%
!	CCTTTCTCTCTTTTCAAAAT+CGG	-	chr6_4:89366823-89366842	Msa1002900:three_prime_UTR	30.0%
!	TTGAAGGAGATTTTGTGAAT+CGG	+	chr6_4:89366985-89367004	None:intergenic	30.0%
	CTCATCTTCAAATCATATCT+AGG	-	chr6_4:89367047-89367066	Msa1002900:three_prime_UTR	30.0%
	TCGAAGAATTTCAAGATTTC+TGG	-	chr6_4:89367201-89367220	Msa1002900:exon	30.0%
!!!	TAAAGGGGTTTGATTTGTTT+TGG	-	chr6_4:89367265-89367284	Msa1002900:CDS	30.0%
!!!	TGATTATGGATTTTGAGATC+TGG	-	chr6_4:89367364-89367383	Msa1002900:intron	30.0%
!!!	GATTATGGATTTTGAGATCT+GGG	-	chr6_4:89367365-89367384	Msa1002900:intron	30.0%
!	TGAGTAAAAGTGTGAAAGTT+TGG	-	chr6_4:89367401-89367420	Msa1002900:intron	30.0%
!	TTATACTGAGGATGGTTTTT+AGG	-	chr6_4:89367436-89367455	Msa1002900:CDS	30.0%
!	TATACTGAGGATGGTTTTTA+GGG	-	chr6_4:89367437-89367456	Msa1002900:CDS	30.0%
!!	TTTTTAGGGATTGAAGGTTT+TGG	-	chr6_4:89367485-89367504	Msa1002900:intron	30.0%
	AGTATAATTCAAAAGTCTCC+AGG	-	chr6_4:89367587-89367606	Msa1002900:intron	30.0%
	GTATAATTCAAAAGTCTCCA+GGG	-	chr6_4:89367588-89367607	Msa1002900:intron	30.0%
	TTTGGTTGCAGTTGTAATTA+TGG	-	chr6_4:89367678-89367697	Msa1002900:intron	30.0%
!	TTTTATGCCAAATCTGCTAT+TGG	-	chr6_4:89367737-89367756	Msa1002900:five_prime_UTR	30.0%
	AATTACACCAATAGCAGATT+TGG	+	chr6_4:89367747-89367766	None:intergenic	30.0%
	ACCATATGATATGTTTGTAC+AGG	-	chr6_4:89367836-89367855	Msa1002900:intron	30.0%
	TCCTGTACAAACATATCATA+TGG	+	chr6_4:89367840-89367859	None:intergenic	30.0%
	ATATGATATGTTTGTACAGG+AGG	-	chr6_4:89367839-89367858	Msa1002900:intron	30.0%
	AGAGGTTCATATTTGCTTAT+TGG	-	chr6_4:89367906-89367925	Msa1002900:intron	30.0%
	TATTGTAGGATGAGACATTA+AGG	-	chr6_4:89368022-89368041	Msa1002900:intron	30.0%
!	TTTTCCCTTAAAAGCTGTAA+CGG	+	chr6_4:89368052-89368071	None:intergenic	30.0%
	GGAAAACATTGGAAGAAAAT+AGG	-	chr6_4:89368066-89368085	Msa1002900:intron	30.0%
!!!	TTGTTGATTTTGTTGGCTAT+TGG	-	chr6_4:89368488-89368507	Msa1002900:five_prime_UTR	30.0%
	ACAAACAAAATCAATAGCAG+AGG	+	chr6_4:89368546-89368565	None:intergenic	30.0%
	TTGTAATCATGTCTCATAAC+TGG	-	chr6_4:89368562-89368581	Msa1002900:five_prime_UTR	30.0%
	GAAGGAGAGAAATGTTACAT+AGG	+	chr6_4:89366804-89366823	None:intergenic	35.0%
!!	CCGATTTTGAAAAGAGAGAA+AGG	+	chr6_4:89366826-89366845	None:intergenic	35.0%
	ATCAGGGTTTGGATTTATGT+TGG	+	chr6_4:89366872-89366891	None:intergenic	35.0%
	TTTGTGAATCGGAGAAGAAA+GGG	+	chr6_4:89366974-89366993	None:intergenic	35.0%
!	TTTTGTGAATCGGAGAAGAA+AGG	+	chr6_4:89366975-89366994	None:intergenic	35.0%
	ACAAAATCTCCTTCAAGATG+CGG	-	chr6_4:89366989-89367008	Msa1002900:three_prime_UTR	35.0%
	AATTGAAAACCGCATCTTGA+AGG	+	chr6_4:89367001-89367020	None:intergenic	35.0%
!	TCAAGATGCGGTTTTCAATT+CGG	-	chr6_4:89367001-89367020	Msa1002900:three_prime_UTR	35.0%
!	TGAAATTCTTCGAAGAGTGT+TGG	+	chr6_4:89367194-89367213	None:intergenic	35.0%
!!!	TTGGTGATTGATGGGTTTTT+AGG	-	chr6_4:89367470-89367489	Msa1002900:intron	35.0%
!!!	TGGTGATTGATGGGTTTTTA+GGG	-	chr6_4:89367471-89367490	Msa1002900:intron	35.0%
!!	GGATATGGATGTTTGTTGTT+TGG	-	chr6_4:89367512-89367531	Msa1002900:intron	35.0%
	AACAAACATTACCGATGTGA+AGG	+	chr6_4:89367644-89367663	None:intergenic	35.0%
!!	AATAGTTGCTGATTCACTTC+TGG	-	chr6_4:89367778-89367797	Msa1002900:five_prime_UTR	35.0%
!!	ATTCAATCCACTTTTCTGGA+AGG	+	chr6_4:89367953-89367972	None:intergenic	35.0%
!!	TCCAGAAAAGTGGATTGAAT+TGG	-	chr6_4:89367953-89367972	Msa1002900:intron	35.0%
!!	CCAGAAAAGTGGATTGAATT+GGG	-	chr6_4:89367954-89367973	Msa1002900:intron	35.0%
!	AATGCCGTTACAGCTTTTAA+GGG	-	chr6_4:89368045-89368064	Msa1002900:intron	35.0%
!!	CAGCTTTTAAGGGAAAACAT+TGG	-	chr6_4:89368055-89368074	Msa1002900:intron	35.0%
	TTTGTCAAATGCATTAGCTC+AGG	+	chr6_4:89368102-89368121	None:intergenic	35.0%
	GTCCAATCTTTCTATTGAAG+TGG	-	chr6_4:89368222-89368241	Msa1002900:intron	35.0%
!	TGCCACTTCAATAGAAAGAT+TGG	+	chr6_4:89368227-89368246	None:intergenic	35.0%
	ATCTTTCTATTGAAGTGGCA+AGG	-	chr6_4:89368227-89368246	Msa1002900:intron	35.0%
	GAAGGAGAGAAATGTTACAT+AGG	+	chr6_4:89366804-89366823	None:intergenic	35.0%
!!	CCGATTTTGAAAAGAGAGAA+AGG	+	chr6_4:89366826-89366845	None:intergenic	35.0%
	ATCAGGGTTTGGATTTATGT+TGG	+	chr6_4:89366872-89366891	None:intergenic	35.0%
	TTTGTGAATCGGAGAAGAAA+GGG	+	chr6_4:89366974-89366993	None:intergenic	35.0%
!	TTTTGTGAATCGGAGAAGAA+AGG	+	chr6_4:89366975-89366994	None:intergenic	35.0%
	ACAAAATCTCCTTCAAGATG+CGG	-	chr6_4:89366989-89367008	Msa1002900:three_prime_UTR	35.0%
	AATTGAAAACCGCATCTTGA+AGG	+	chr6_4:89367001-89367020	None:intergenic	35.0%
!	TCAAGATGCGGTTTTCAATT+CGG	-	chr6_4:89367001-89367020	Msa1002900:three_prime_UTR	35.0%
!	TGAAATTCTTCGAAGAGTGT+TGG	+	chr6_4:89367194-89367213	None:intergenic	35.0%
!!!	TTGGTGATTGATGGGTTTTT+AGG	-	chr6_4:89367470-89367489	Msa1002900:intron	35.0%
!!!	TGGTGATTGATGGGTTTTTA+GGG	-	chr6_4:89367471-89367490	Msa1002900:intron	35.0%
!!	GGATATGGATGTTTGTTGTT+TGG	-	chr6_4:89367512-89367531	Msa1002900:intron	35.0%
	AACAAACATTACCGATGTGA+AGG	+	chr6_4:89367644-89367663	None:intergenic	35.0%
!!	AATAGTTGCTGATTCACTTC+TGG	-	chr6_4:89367778-89367797	Msa1002900:five_prime_UTR	35.0%
!!	ATTCAATCCACTTTTCTGGA+AGG	+	chr6_4:89367953-89367972	None:intergenic	35.0%
!!	TCCAGAAAAGTGGATTGAAT+TGG	-	chr6_4:89367953-89367972	Msa1002900:intron	35.0%
!!	CCAGAAAAGTGGATTGAATT+GGG	-	chr6_4:89367954-89367973	Msa1002900:intron	35.0%
!	AATGCCGTTACAGCTTTTAA+GGG	-	chr6_4:89368045-89368064	Msa1002900:intron	35.0%
!!	CAGCTTTTAAGGGAAAACAT+TGG	-	chr6_4:89368055-89368074	Msa1002900:intron	35.0%
	TTTGTCAAATGCATTAGCTC+AGG	+	chr6_4:89368102-89368121	None:intergenic	35.0%
	GTCCAATCTTTCTATTGAAG+TGG	-	chr6_4:89368222-89368241	Msa1002900:intron	35.0%
!	TGCCACTTCAATAGAAAGAT+TGG	+	chr6_4:89368227-89368246	None:intergenic	35.0%
	ATCTTTCTATTGAAGTGGCA+AGG	-	chr6_4:89368227-89368246	Msa1002900:intron	35.0%
	TGTTTGGGCTTCTCTCTAAT+TGG	+	chr6_4:89366728-89366747	None:intergenic	40.0%
!	GTTTGGATTTATGTTGGTGG+AGG	+	chr6_4:89366866-89366885	None:intergenic	40.0%
	AGGGTTTGGATTTATGTTGG+TGG	+	chr6_4:89366869-89366888	None:intergenic	40.0%
	AGAAGGGGAAAATAGTGTGA+GGG	+	chr6_4:89366902-89366921	None:intergenic	40.0%
	AAGAAGGGGAAAATAGTGTG+AGG	+	chr6_4:89366903-89366922	None:intergenic	40.0%
	AGGGAAGGAAGATAAAGAAG+GGG	+	chr6_4:89366917-89366936	None:intergenic	40.0%
	GAGGGAAGGAAGATAAAGAA+GGG	+	chr6_4:89366918-89366937	None:intergenic	40.0%
	TGAGGGAAGGAAGATAAAGA+AGG	+	chr6_4:89366919-89366938	None:intergenic	40.0%
!!	GATGGGTTTTTAGGGATTGA+AGG	-	chr6_4:89367479-89367498	Msa1002900:intron	40.0%
!!	GAAGGTTTTGGTGAAGGATA+TGG	-	chr6_4:89367497-89367516	Msa1002900:intron	40.0%
	AAACATTACCGATGTGAAGG+CGG	+	chr6_4:89367641-89367660	None:intergenic	40.0%
!	TTTTCCTCCTTCCAGAAAAG+TGG	-	chr6_4:89367943-89367962	Msa1002900:intron	40.0%
!!	CCCAATTCAATCCACTTTTC+TGG	+	chr6_4:89367957-89367976	None:intergenic	40.0%
!	GAATGCCGTTACAGCTTTTA+AGG	-	chr6_4:89368044-89368063	Msa1002900:intron	40.0%
	ATTGAAGTGGCAAGGATGAT+TGG	-	chr6_4:89368235-89368254	Msa1002900:intron	40.0%
	TGTTTGGGCTTCTCTCTAAT+TGG	+	chr6_4:89366728-89366747	None:intergenic	40.0%
!	GTTTGGATTTATGTTGGTGG+AGG	+	chr6_4:89366866-89366885	None:intergenic	40.0%
	AGGGTTTGGATTTATGTTGG+TGG	+	chr6_4:89366869-89366888	None:intergenic	40.0%
	AGAAGGGGAAAATAGTGTGA+GGG	+	chr6_4:89366902-89366921	None:intergenic	40.0%
	AAGAAGGGGAAAATAGTGTG+AGG	+	chr6_4:89366903-89366922	None:intergenic	40.0%
	AGGGAAGGAAGATAAAGAAG+GGG	+	chr6_4:89366917-89366936	None:intergenic	40.0%
	GAGGGAAGGAAGATAAAGAA+GGG	+	chr6_4:89366918-89366937	None:intergenic	40.0%
	TGAGGGAAGGAAGATAAAGA+AGG	+	chr6_4:89366919-89366938	None:intergenic	40.0%
!!	GATGGGTTTTTAGGGATTGA+AGG	-	chr6_4:89367479-89367498	Msa1002900:intron	40.0%
!!	GAAGGTTTTGGTGAAGGATA+TGG	-	chr6_4:89367497-89367516	Msa1002900:intron	40.0%
	AAACATTACCGATGTGAAGG+CGG	+	chr6_4:89367641-89367660	None:intergenic	40.0%
!	TTTTCCTCCTTCCAGAAAAG+TGG	-	chr6_4:89367943-89367962	Msa1002900:intron	40.0%
!!	CCCAATTCAATCCACTTTTC+TGG	+	chr6_4:89367957-89367976	None:intergenic	40.0%
!	GAATGCCGTTACAGCTTTTA+AGG	-	chr6_4:89368044-89368063	Msa1002900:intron	40.0%
	ATTGAAGTGGCAAGGATGAT+TGG	-	chr6_4:89368235-89368254	Msa1002900:intron	40.0%
!	TGGATTTATGTTGGTGGAGG+TGG	+	chr6_4:89366863-89366882	None:intergenic	45.0%
	AGGGAGAAGATATCAGGGTT+TGG	+	chr6_4:89366883-89366902	None:intergenic	45.0%
	GTGTGAGGGAGAAGATATCA+GGG	+	chr6_4:89366888-89366907	None:intergenic	45.0%
	AGTGTGAGGGAGAAGATATC+AGG	+	chr6_4:89366889-89366908	None:intergenic	45.0%
!	AAAGAGAAGCATGTGAGGGA+AGG	+	chr6_4:89366932-89366951	None:intergenic	45.0%
!	TGGCAAAGAGAAGCATGTGA+GGG	+	chr6_4:89366936-89366955	None:intergenic	45.0%
!!	GGGATTGAAGGTTTTGGTGA+AGG	-	chr6_4:89367491-89367510	Msa1002900:intron	45.0%
	TCTTGCTCATCTGTGTCTGA+AGG	-	chr6_4:89367557-89367576	Msa1002900:intron	45.0%
	GGAGGAGAACAAGTCCTATA+AGG	-	chr6_4:89367857-89367876	Msa1002900:intron	45.0%
	AAGTCCTATAAGGCACGCTA+AGG	-	chr6_4:89367867-89367886	Msa1002900:intron	45.0%
	AGTCCTATAAGGCACGCTAA+GGG	-	chr6_4:89367868-89367887	Msa1002900:intron	45.0%
!!	CAATCCACTTTTCTGGAAGG+AGG	+	chr6_4:89367950-89367969	None:intergenic	45.0%
!	TGGATTTATGTTGGTGGAGG+TGG	+	chr6_4:89366863-89366882	None:intergenic	45.0%
	AGGGAGAAGATATCAGGGTT+TGG	+	chr6_4:89366883-89366902	None:intergenic	45.0%
	GTGTGAGGGAGAAGATATCA+GGG	+	chr6_4:89366888-89366907	None:intergenic	45.0%
	AGTGTGAGGGAGAAGATATC+AGG	+	chr6_4:89366889-89366908	None:intergenic	45.0%
!	AAAGAGAAGCATGTGAGGGA+AGG	+	chr6_4:89366932-89366951	None:intergenic	45.0%
!	TGGCAAAGAGAAGCATGTGA+GGG	+	chr6_4:89366936-89366955	None:intergenic	45.0%
!!	GGGATTGAAGGTTTTGGTGA+AGG	-	chr6_4:89367491-89367510	Msa1002900:intron	45.0%
	TCTTGCTCATCTGTGTCTGA+AGG	-	chr6_4:89367557-89367576	Msa1002900:intron	45.0%
	GGAGGAGAACAAGTCCTATA+AGG	-	chr6_4:89367857-89367876	Msa1002900:intron	45.0%
	AAGTCCTATAAGGCACGCTA+AGG	-	chr6_4:89367867-89367886	Msa1002900:intron	45.0%
	AGTCCTATAAGGCACGCTAA+GGG	-	chr6_4:89367868-89367887	Msa1002900:intron	45.0%
!!	CAATCCACTTTTCTGGAAGG+AGG	+	chr6_4:89367950-89367969	None:intergenic	45.0%
!	GTGGCAAAGAGAAGCATGTG+AGG	+	chr6_4:89366937-89366956	None:intergenic	50.0%
	AGAAAGGGAGGAGGTTCAGA+TGG	+	chr6_4:89366959-89366978	None:intergenic	50.0%
	AATCGGAGAAGAAAGGGAGG+AGG	+	chr6_4:89366968-89366987	None:intergenic	50.0%
	GTGAATCGGAGAAGAAAGGG+AGG	+	chr6_4:89366971-89366990	None:intergenic	50.0%
	TTTGCCTCTGCACTTTGAGC+TGG	-	chr6_4:89368512-89368531	Msa1002900:five_prime_UTR	50.0%
	ATGTCCAGCTCAAAGTGCAG+AGG	+	chr6_4:89368519-89368538	None:intergenic	50.0%
!	GTGGCAAAGAGAAGCATGTG+AGG	+	chr6_4:89366937-89366956	None:intergenic	50.0%
	AGAAAGGGAGGAGGTTCAGA+TGG	+	chr6_4:89366959-89366978	None:intergenic	50.0%
	AATCGGAGAAGAAAGGGAGG+AGG	+	chr6_4:89366968-89366987	None:intergenic	50.0%
	GTGAATCGGAGAAGAAAGGG+AGG	+	chr6_4:89366971-89366990	None:intergenic	50.0%
	TTTGCCTCTGCACTTTGAGC+TGG	-	chr6_4:89368512-89368531	Msa1002900:five_prime_UTR	50.0%
	ATGTCCAGCTCAAAGTGCAG+AGG	+	chr6_4:89368519-89368538	None:intergenic	50.0%
	AAGGGAGGAGGTTCAGATGG+TGG	+	chr6_4:89366956-89366975	None:intergenic	55.0%
	TCCAGGGATATGTAGCCCCA+TGG	-	chr6_4:89367604-89367623	Msa1002900:intron	55.0%
	CATCCACACCGCCTTCACAT+CGG	-	chr6_4:89367630-89367649	Msa1002900:intron	55.0%
	TTACCGATGTGAAGGCGGTG+TGG	+	chr6_4:89367636-89367655	None:intergenic	55.0%
	CCACCCTTAGCGTGCCTTAT+AGG	+	chr6_4:89367874-89367893	None:intergenic	55.0%
	CCTATAAGGCACGCTAAGGG+TGG	-	chr6_4:89367871-89367890	Msa1002900:intron	55.0%
	TAAGGCACGCTAAGGGTGGT+TGG	-	chr6_4:89367875-89367894	Msa1002900:intron	55.0%
	AAGGGAGGAGGTTCAGATGG+TGG	+	chr6_4:89366956-89366975	None:intergenic	55.0%
	TCCAGGGATATGTAGCCCCA+TGG	-	chr6_4:89367604-89367623	Msa1002900:intron	55.0%
	CATCCACACCGCCTTCACAT+CGG	-	chr6_4:89367630-89367649	Msa1002900:intron	55.0%
	TTACCGATGTGAAGGCGGTG+TGG	+	chr6_4:89367636-89367655	None:intergenic	55.0%
	CCACCCTTAGCGTGCCTTAT+AGG	+	chr6_4:89367874-89367893	None:intergenic	55.0%
	CCTATAAGGCACGCTAAGGG+TGG	-	chr6_4:89367871-89367890	Msa1002900:intron	55.0%
	TAAGGCACGCTAAGGGTGGT+TGG	-	chr6_4:89367875-89367894	Msa1002900:intron	55.0%
	GCCATGGGGCTACATATCCC+TGG	+	chr6_4:89367608-89367627	None:intergenic	60.0%
	GCGGTGTGGATGTAGCCATG+GGG	+	chr6_4:89367622-89367641	None:intergenic	60.0%
	GGCGGTGTGGATGTAGCCAT+GGG	+	chr6_4:89367623-89367642	None:intergenic	60.0%
	AGGCGGTGTGGATGTAGCCA+TGG	+	chr6_4:89367624-89367643	None:intergenic	60.0%
	GGGTGGTTGGACAGCAGAAG+AGG	-	chr6_4:89367888-89367907	Msa1002900:intron	60.0%
	GCCATGGGGCTACATATCCC+TGG	+	chr6_4:89367608-89367627	None:intergenic	60.0%
	GCGGTGTGGATGTAGCCATG+GGG	+	chr6_4:89367622-89367641	None:intergenic	60.0%
	GGCGGTGTGGATGTAGCCAT+GGG	+	chr6_4:89367623-89367642	None:intergenic	60.0%
	AGGCGGTGTGGATGTAGCCA+TGG	+	chr6_4:89367624-89367643	None:intergenic	60.0%
	GGGTGGTTGGACAGCAGAAG+AGG	-	chr6_4:89367888-89367907	Msa1002900:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	89366705	89368634	89366705	ID=Msa1002900;Name=Msa1002900
chr6_4	mRNA	89366705	89368634	89366705	ID=Msa1002900-mRNA-1;Parent=Msa1002900;Name=Msa1002900-mRNA-1;_AED=0.32;_eAED=0.32;_QI=496\|1\|1\|1\|0.66\|0.5\|4\|509\|58
chr6_4	exon	89366705	89367309	89366705	ID=Msa1002900-mRNA-1:exon:17442;Parent=Msa1002900-mRNA-1
chr6_4	exon	89367430	89367481	89367430	ID=Msa1002900-mRNA-1:exon:17441;Parent=Msa1002900-mRNA-1
chr6_4	exon	89367688	89367847	89367688	ID=Msa1002900-mRNA-1:exon:17440;Parent=Msa1002900-mRNA-1
chr6_4	exon	89368270	89368634	89368270	ID=Msa1002900-mRNA-1:exon:17439;Parent=Msa1002900-mRNA-1
chr6_4	five_prime_UTR	89368270	89368634	89368270	ID=Msa1002900-mRNA-1:five_prime_utr;Parent=Msa1002900-mRNA-1
chr6_4	five_prime_UTR	89367717	89367847	89367717	ID=Msa1002900-mRNA-1:five_prime_utr;Parent=Msa1002900-mRNA-1
chr6_4	CDS	89367688	89367716	89367688	ID=Msa1002900-mRNA-1:cds;Parent=Msa1002900-mRNA-1
chr6_4	CDS	89367430	89367481	89367430	ID=Msa1002900-mRNA-1:cds;Parent=Msa1002900-mRNA-1
chr6_4	CDS	89367214	89367309	89367214	ID=Msa1002900-mRNA-1:cds;Parent=Msa1002900-mRNA-1
chr6_4	three_prime_UTR	89366705	89367213	89366705	ID=Msa1002900-mRNA-1:three_prime_utr;Parent=Msa1002900-mRNA-1

Gene Sequence	>Msa1002900 ATGGCTACATCCACACCGCCTTCACATCGGAGGAGAACAAGTCCTATAAGGCACGCTAAGGGTGGTTGGACAGCAGAAGAGGATGAGACATTAAGGAATGCCGTTACAGCTTTTAAGGGAAAACATTGGAAGAAAATAGGTAACTATTTGCCTGAGCTAATGCATTTGACAAATTAA
Protein sequence	>Msa1002900 MATSTPPSHRRRTSPIRHAKGGWTAEEDETLRNAVTAFKGKHWKKIGNYLPELMHLTN*