AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1419060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1419060 MtrunA17_Chr7g0238631 78.431 51 11 0 2 52 43 93 2.64e-22 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1419060 AT2G13960.1 65.116 43 15 0 10 52 65 107 2.13e-15 65.5
Msa1419060 AT2G13960.2 65.116 43 15 0 10 52 30 72 2.75e-15 64.3
Msa1419060 AT5G02320.1 65.116 43 15 0 10 52 65 107 2.78e-14 65.1
Msa1419060 AT5G02320.2 65.116 43 15 0 10 52 65 107 2.78e-14 65.1
Msa1419060 AT5G02320.3 65.116 43 15 0 10 52 5 47 3.82e-14 64.7
Msa1419060 AT3G09370.2 61.224 49 16 1 7 52 67 115 1.13e-13 63.5
Msa1419060 AT3G09370.1 56.604 53 20 1 7 56 62 114 1.14e-13 63.5
Msa1419060 AT3G09370.3 61.224 49 16 1 7 52 75 123 1.15e-13 63.5

Find 61 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTAATTGGTCAATTATT+TGG 0.166929 tig0024668:-29014 None:intergenic
CCACCCTTAGCGTGCCTTAT+AGG 0.202151 tig0024668:-30172 None:intergenic
TATTGTAGGATGAGACATTA+AGG 0.293653 tig0024668:+30323 Msa1419060:intron
TGGATTTATGTTGGTGGAGG+TGG 0.309935 tig0024668:-29161 None:intergenic
GGAAAACATTGGAAGAAAAT+AGG 0.311225 tig0024668:+30367 Msa1419060:CDS
TGTTTGGGCTTCTCTCTAAT+TGG 0.328403 tig0024668:-29026 None:intergenic
CTCATCTTCAAATCATATCT+AGG 0.331174 tig0024668:+29348 Msa1419060:five_prime_UTR
AGTGTGAGGGAGAAGATATC+AGG 0.349806 tig0024668:-29187 None:intergenic
TTGTAATCATGTCTCATAAC+TGG 0.356897 tig0024668:+30863 Msa1419060:three_prime_UTR
TGCCACTTCAATAGAAAGAT+TGG 0.375619 tig0024668:-30525 None:intergenic
GGATATGGATGTTTGTTGTT+TGG 0.389621 tig0024668:+29813 Msa1419060:five_prime_UTR
ATTGAAGTGGCAAGGATGAT+TGG 0.406073 tig0024668:+30536 Msa1419060:three_prime_UTR
GGAGGAGAACAAGTCCTATA+AGG 0.409948 tig0024668:+30158 Msa1419060:intron
TTTGCCTCTGCACTTTGAGC+TGG 0.415507 tig0024668:+30813 Msa1419060:three_prime_UTR
GTAATGTTTCATACATTTCA+AGG 0.423379 tig0024668:-30712 None:intergenic
AGGGAGAAGATATCAGGGTT+TGG 0.428253 tig0024668:-29181 None:intergenic
AGGGTTTGGATTTATGTTGG+TGG 0.431049 tig0024668:-29167 None:intergenic
AATTGAAAACCGCATCTTGA+AGG 0.449911 tig0024668:-29299 None:intergenic
AGTATAATTCAAAAGTCTCC+AGG 0.458256 tig0024668:+29888 Msa1419060:five_prime_UTR
TTTGTCAAATGCATTAGCTC+AGG 0.463353 tig0024668:-30400 None:intergenic
ATCAGGGTTTGGATTTATGT+TGG 0.464167 tig0024668:-29170 None:intergenic
TTTGTGAATCGGAGAAGAAA+GGG 0.464883 tig0024668:-29272 None:intergenic
GTCCAATCTTTCTATTGAAG+TGG 0.468597 tig0024668:+30523 Msa1419060:three_prime_UTR
AGAAAGGGAGGAGGTTCAGA+TGG 0.474386 tig0024668:-29257 None:intergenic
AGGCGGTGTGGATGTAGCCA+TGG 0.485223 tig0024668:-29922 None:intergenic
GTTTGGATTTATGTTGGTGG+AGG 0.500329 tig0024668:-29164 None:intergenic
GGCGGTGTGGATGTAGCCAT+GGG 0.512625 tig0024668:-29921 None:intergenic
GAAGGAGAGAAATGTTACAT+AGG 0.517797 tig0024668:-29102 None:intergenic
GAGGGAAGGAAGATAAAGAA+GGG 0.520886 tig0024668:-29216 None:intergenic
GAAATGTTACATAGGAAATG+TGG 0.521689 tig0024668:-29094 None:intergenic
GTGTGAGGGAGAAGATATCA+GGG 0.524168 tig0024668:-29186 None:intergenic
TGAGGGAAGGAAGATAAAGA+AGG 0.525432 tig0024668:-29217 None:intergenic
AAGTCCTATAAGGCACGCTA+AGG 0.528953 tig0024668:+30168 Msa1419060:CDS
TCATCTTCAAATCATATCTA+GGG 0.535900 tig0024668:+29349 Msa1419060:five_prime_UTR
GATTATGAAGTAAAGACAAA+AGG 0.537647 tig0024668:-30563 None:intergenic
ATCTTTCTATTGAAGTGGCA+AGG 0.545324 tig0024668:+30528 Msa1419060:three_prime_UTR
GCCATGGGGCTACATATCCC+TGG 0.552664 tig0024668:-29906 None:intergenic
TCTTGCTCATCTGTGTCTGA+AGG 0.559484 tig0024668:+29858 Msa1419060:five_prime_UTR
ACAAAATCTCCTTCAAGATG+CGG 0.561408 tig0024668:+29290 Msa1419060:five_prime_UTR
GTGAATCGGAGAAGAAAGGG+AGG 0.561423 tig0024668:-29269 None:intergenic
TAAGGCACGCTAAGGGTGGT+TGG 0.562887 tig0024668:+30176 Msa1419060:CDS
AAAGAGAAGCATGTGAGGGA+AGG 0.591322 tig0024668:-29230 None:intergenic
AACAAACATTACCGATGTGA+AGG 0.591884 tig0024668:-29942 None:intergenic
AGAAGGGGAAAATAGTGTGA+GGG 0.595581 tig0024668:-29200 None:intergenic
GTGGCAAAGAGAAGCATGTG+AGG 0.601561 tig0024668:-29235 None:intergenic
GGGTGGTTGGACAGCAGAAG+AGG 0.605706 tig0024668:+30189 Msa1419060:CDS
AGGGAAGGAAGATAAAGAAG+GGG 0.607295 tig0024668:-29215 None:intergenic
CCTATAAGGCACGCTAAGGG+TGG 0.618742 tig0024668:+30172 Msa1419060:CDS
AGTCCTATAAGGCACGCTAA+GGG 0.620051 tig0024668:+30169 Msa1419060:CDS
AATCGGAGAAGAAAGGGAGG+AGG 0.620302 tig0024668:-29266 None:intergenic
AAGAAGGGGAAAATAGTGTG+AGG 0.621252 tig0024668:-29201 None:intergenic
AAGGGAGGAGGTTCAGATGG+TGG 0.627661 tig0024668:-29254 None:intergenic
ACAAACAAAATCAATAGCAG+AGG 0.629915 tig0024668:-30844 None:intergenic
GTATAATTCAAAAGTCTCCA+GGG 0.641290 tig0024668:+29889 Msa1419060:five_prime_UTR
TCCAGGGATATGTAGCCCCA+TGG 0.641883 tig0024668:+29905 Msa1419060:exon
ATGTCCAGCTCAAAGTGCAG+AGG 0.663657 tig0024668:-30817 None:intergenic
CATCCACACCGCCTTCACAT+CGG 0.670345 tig0024668:+29931 Msa1419060:CDS
TGGCAAAGAGAAGCATGTGA+GGG 0.676512 tig0024668:-29234 None:intergenic
AAACATTACCGATGTGAAGG+CGG 0.687678 tig0024668:-29939 None:intergenic
TTACCGATGTGAAGGCGGTG+TGG 0.693836 tig0024668:-29934 None:intergenic
GCGGTGTGGATGTAGCCATG+GGG 0.784527 tig0024668:-29920 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTAGTAATTAAAGA+TGG - tig0024668:29439-29458 None:intergenic 10.0%
!!! ATTTTTTTTTTTTTATACTG+AGG + tig0024668:29725-29744 Msa1419060:intron 10.0%
!! AAATAATTAGTAATTAAAGA+TGG - tig0024668:29439-29458 None:intergenic 10.0%
!!! ATTTTTTTTTTTTTATACTG+AGG + tig0024668:29725-29744 Msa1419060:intron 10.0%
!! AATATAAATGCATTGAATAA+AGG - tig0024668:29389-29408 None:intergenic 15.0%
!!! TTTTTTTATTGGTTTTGTTT+TGG + tig0024668:29599-29618 Msa1419060:intron 15.0%
!!! GATAAAGATTTTGTTTTTTT+GGG + tig0024668:29621-29640 Msa1419060:intron 15.0%
!! AATATAAATGCATTGAATAA+AGG - tig0024668:29389-29408 None:intergenic 15.0%
!!! TTTTTTTATTGGTTTTGTTT+TGG + tig0024668:29599-29618 Msa1419060:intron 15.0%
!!! GATAAAGATTTTGTTTTTTT+GGG + tig0024668:29621-29640 Msa1419060:intron 15.0%
!!! GTCAAAAAACGTTTTTTTAT+TGG + tig0024668:29588-29607 Msa1419060:intron 20.0%
!!! GGATAAAGATTTTGTTTTTT+TGG + tig0024668:29620-29639 Msa1419060:intron 20.0%
!!! TTTTTTTGGGAAAAAATTGA+AGG + tig0024668:29634-29653 Msa1419060:intron 20.0%
!!! TTTTTTTTTTATACTGAGGA+TGG + tig0024668:29729-29748 Msa1419060:intron 20.0%
!!! TTTTTAGGGTTTTTTATGTT+TGG + tig0024668:29752-29771 Msa1419060:intron 20.0%
!!! TGTTATGTTATTTACGTTTT+TGG + tig0024668:29961-29980 Msa1419060:intron 20.0%
!!! CATTTTAATTAGAAAGATTG+AGG + tig0024668:30600-30619 Msa1419060:three_prime_UTR 20.0%
!!! GTCAAAAAACGTTTTTTTAT+TGG + tig0024668:29588-29607 Msa1419060:intron 20.0%
!!! GGATAAAGATTTTGTTTTTT+TGG + tig0024668:29620-29639 Msa1419060:intron 20.0%
!!! TTTTTTTGGGAAAAAATTGA+AGG + tig0024668:29634-29653 Msa1419060:intron 20.0%
!!! TTTTTTTTTTATACTGAGGA+TGG + tig0024668:29729-29748 Msa1419060:intron 20.0%
!!! TTTTTAGGGTTTTTTATGTT+TGG + tig0024668:29752-29771 Msa1419060:intron 20.0%
!!! TGTTATGTTATTTACGTTTT+TGG + tig0024668:29961-29980 Msa1419060:intron 20.0%
!!! CATTTTAATTAGAAAGATTG+AGG + tig0024668:30600-30619 Msa1419060:three_prime_UTR 20.0%
! TCTCTAATTGGTCAATTATT+TGG - tig0024668:29017-29036 None:intergenic 25.0%
! TCATCTTCAAATCATATCTA+GGG + tig0024668:29349-29368 Msa1419060:five_prime_UTR 25.0%
!! TTGATTGAGTTTTTGTGTTT+TGG + tig0024668:29542-29561 Msa1419060:intron 25.0%
!!! AGTTTTTGTGTTTTGGATAA+AGG + tig0024668:29549-29568 Msa1419060:intron 25.0%
!!! GTTTTTGTGTTTTGGATAAA+GGG + tig0024668:29550-29569 Msa1419060:intron 25.0%
!!! TTTTTGTGTTTTGGATAAAG+GGG + tig0024668:29551-29570 Msa1419060:intron 25.0%
!!! TGAAGGTTTTGATTTGATTA+TGG + tig0024668:29651-29670 Msa1419060:intron 25.0%
!!! TTTTTATGTTTGGTGATTGA+TGG + tig0024668:29762-29781 Msa1419060:intron 25.0%
!!! TTTTATGTTTGGTGATTGAT+GGG + tig0024668:29763-29782 Msa1419060:intron 25.0%
! ATTGAAGAACACAATAAGAA+CGG - tig0024668:30302-30321 None:intergenic 25.0%
! TGTGTTCTTCAATTTATTGT+AGG + tig0024668:30309-30328 Msa1419060:intron 25.0%
! GATTATGAAGTAAAGACAAA+AGG - tig0024668:30566-30585 None:intergenic 25.0%
! GTAATGTTTCATACATTTCA+AGG - tig0024668:30715-30734 None:intergenic 25.0%
!!! GTGTTTTTTGTTGATTTTGT+TGG + tig0024668:30782-30801 Msa1419060:three_prime_UTR 25.0%
! TCTCTAATTGGTCAATTATT+TGG - tig0024668:29017-29036 None:intergenic 25.0%
! TCATCTTCAAATCATATCTA+GGG + tig0024668:29349-29368 Msa1419060:five_prime_UTR 25.0%
!! TTGATTGAGTTTTTGTGTTT+TGG + tig0024668:29542-29561 Msa1419060:intron 25.0%
!!! AGTTTTTGTGTTTTGGATAA+AGG + tig0024668:29549-29568 Msa1419060:intron 25.0%
!!! GTTTTTGTGTTTTGGATAAA+GGG + tig0024668:29550-29569 Msa1419060:intron 25.0%
!!! TTTTTGTGTTTTGGATAAAG+GGG + tig0024668:29551-29570 Msa1419060:intron 25.0%
!!! TGAAGGTTTTGATTTGATTA+TGG + tig0024668:29651-29670 Msa1419060:intron 25.0%
!!! TTTTTATGTTTGGTGATTGA+TGG + tig0024668:29762-29781 Msa1419060:intron 25.0%
!!! TTTTATGTTTGGTGATTGAT+GGG + tig0024668:29763-29782 Msa1419060:intron 25.0%
! ATTGAAGAACACAATAAGAA+CGG - tig0024668:30302-30321 None:intergenic 25.0%
! TGTGTTCTTCAATTTATTGT+AGG + tig0024668:30309-30328 Msa1419060:intron 25.0%
! GATTATGAAGTAAAGACAAA+AGG - tig0024668:30566-30585 None:intergenic 25.0%
! GTAATGTTTCATACATTTCA+AGG - tig0024668:30715-30734 None:intergenic 25.0%
!!! GTGTTTTTTGTTGATTTTGT+TGG + tig0024668:30782-30801 Msa1419060:three_prime_UTR 25.0%
! TTTTGCTTGTAGTCATGTTT+GGG - tig0024668:29044-29063 None:intergenic 30.0%
! TTTTTGCTTGTAGTCATGTT+TGG - tig0024668:29045-29064 None:intergenic 30.0%
GAAATGTTACATAGGAAATG+TGG - tig0024668:29097-29116 None:intergenic 30.0%
!! TTTTGAAAAGAGAGAAAGGA+AGG - tig0024668:29123-29142 None:intergenic 30.0%
! CCTTTCTCTCTTTTCAAAAT+CGG + tig0024668:29124-29143 Msa1419060:five_prime_UTR 30.0%
! TTGAAGGAGATTTTGTGAAT+CGG - tig0024668:29286-29305 None:intergenic 30.0%
CTCATCTTCAAATCATATCT+AGG + tig0024668:29348-29367 Msa1419060:five_prime_UTR 30.0%
TCGAAGAATTTCAAGATTTC+TGG + tig0024668:29502-29521 Msa1419060:intron 30.0%
!!! TAAAGGGGTTTGATTTGTTT+TGG + tig0024668:29566-29585 Msa1419060:intron 30.0%
!!! TGATTATGGATTTTGAGATC+TGG + tig0024668:29665-29684 Msa1419060:intron 30.0%
!!! GATTATGGATTTTGAGATCT+GGG + tig0024668:29666-29685 Msa1419060:intron 30.0%
! TGAGTAAAAGTGTGAAAGTT+TGG + tig0024668:29702-29721 Msa1419060:intron 30.0%
! TTATACTGAGGATGGTTTTT+AGG + tig0024668:29737-29756 Msa1419060:intron 30.0%
! TATACTGAGGATGGTTTTTA+GGG + tig0024668:29738-29757 Msa1419060:intron 30.0%
!! TTTTTAGGGATTGAAGGTTT+TGG + tig0024668:29786-29805 Msa1419060:intron 30.0%
AGTATAATTCAAAAGTCTCC+AGG + tig0024668:29888-29907 Msa1419060:five_prime_UTR 30.0%
GTATAATTCAAAAGTCTCCA+GGG + tig0024668:29889-29908 Msa1419060:five_prime_UTR 30.0%
TTTGGTTGCAGTTGTAATTA+TGG + tig0024668:29979-29998 Msa1419060:intron 30.0%
! TTTTATGCCAAATCTGCTAT+TGG + tig0024668:30038-30057 Msa1419060:intron 30.0%
AATTACACCAATAGCAGATT+TGG - tig0024668:30048-30067 None:intergenic 30.0%
ACCATATGATATGTTTGTAC+AGG + tig0024668:30137-30156 Msa1419060:intron 30.0%
TCCTGTACAAACATATCATA+TGG - tig0024668:30141-30160 None:intergenic 30.0%
ATATGATATGTTTGTACAGG+AGG + tig0024668:30140-30159 Msa1419060:intron 30.0%
AGAGGTTCATATTTGCTTAT+TGG + tig0024668:30207-30226 Msa1419060:intron 30.0%
TATTGTAGGATGAGACATTA+AGG + tig0024668:30323-30342 Msa1419060:intron 30.0%
! TTTTCCCTTAAAAGCTGTAA+CGG - tig0024668:30353-30372 None:intergenic 30.0%
GGAAAACATTGGAAGAAAAT+AGG + tig0024668:30367-30386 Msa1419060:CDS 30.0%
!!! TTGTTGATTTTGTTGGCTAT+TGG + tig0024668:30789-30808 Msa1419060:three_prime_UTR 30.0%
ACAAACAAAATCAATAGCAG+AGG - tig0024668:30847-30866 None:intergenic 30.0%
TTGTAATCATGTCTCATAAC+TGG + tig0024668:30863-30882 Msa1419060:three_prime_UTR 30.0%
! TTTTGCTTGTAGTCATGTTT+GGG - tig0024668:29044-29063 None:intergenic 30.0%
! TTTTTGCTTGTAGTCATGTT+TGG - tig0024668:29045-29064 None:intergenic 30.0%
GAAATGTTACATAGGAAATG+TGG - tig0024668:29097-29116 None:intergenic 30.0%
!! TTTTGAAAAGAGAGAAAGGA+AGG - tig0024668:29123-29142 None:intergenic 30.0%
! CCTTTCTCTCTTTTCAAAAT+CGG + tig0024668:29124-29143 Msa1419060:five_prime_UTR 30.0%
! TTGAAGGAGATTTTGTGAAT+CGG - tig0024668:29286-29305 None:intergenic 30.0%
CTCATCTTCAAATCATATCT+AGG + tig0024668:29348-29367 Msa1419060:five_prime_UTR 30.0%
TCGAAGAATTTCAAGATTTC+TGG + tig0024668:29502-29521 Msa1419060:intron 30.0%
!!! TAAAGGGGTTTGATTTGTTT+TGG + tig0024668:29566-29585 Msa1419060:intron 30.0%
!!! TGATTATGGATTTTGAGATC+TGG + tig0024668:29665-29684 Msa1419060:intron 30.0%
!!! GATTATGGATTTTGAGATCT+GGG + tig0024668:29666-29685 Msa1419060:intron 30.0%
! TGAGTAAAAGTGTGAAAGTT+TGG + tig0024668:29702-29721 Msa1419060:intron 30.0%
! TTATACTGAGGATGGTTTTT+AGG + tig0024668:29737-29756 Msa1419060:intron 30.0%
! TATACTGAGGATGGTTTTTA+GGG + tig0024668:29738-29757 Msa1419060:intron 30.0%
!! TTTTTAGGGATTGAAGGTTT+TGG + tig0024668:29786-29805 Msa1419060:intron 30.0%
AGTATAATTCAAAAGTCTCC+AGG + tig0024668:29888-29907 Msa1419060:five_prime_UTR 30.0%
GTATAATTCAAAAGTCTCCA+GGG + tig0024668:29889-29908 Msa1419060:five_prime_UTR 30.0%
TTTGGTTGCAGTTGTAATTA+TGG + tig0024668:29979-29998 Msa1419060:intron 30.0%
! TTTTATGCCAAATCTGCTAT+TGG + tig0024668:30038-30057 Msa1419060:intron 30.0%
AATTACACCAATAGCAGATT+TGG - tig0024668:30048-30067 None:intergenic 30.0%
ACCATATGATATGTTTGTAC+AGG + tig0024668:30137-30156 Msa1419060:intron 30.0%
TCCTGTACAAACATATCATA+TGG - tig0024668:30141-30160 None:intergenic 30.0%
ATATGATATGTTTGTACAGG+AGG + tig0024668:30140-30159 Msa1419060:intron 30.0%
AGAGGTTCATATTTGCTTAT+TGG + tig0024668:30207-30226 Msa1419060:intron 30.0%
TATTGTAGGATGAGACATTA+AGG + tig0024668:30323-30342 Msa1419060:intron 30.0%
! TTTTCCCTTAAAAGCTGTAA+CGG - tig0024668:30353-30372 None:intergenic 30.0%
GGAAAACATTGGAAGAAAAT+AGG + tig0024668:30367-30386 Msa1419060:CDS 30.0%
!!! TTGTTGATTTTGTTGGCTAT+TGG + tig0024668:30789-30808 Msa1419060:three_prime_UTR 30.0%
ACAAACAAAATCAATAGCAG+AGG - tig0024668:30847-30866 None:intergenic 30.0%
TTGTAATCATGTCTCATAAC+TGG + tig0024668:30863-30882 Msa1419060:three_prime_UTR 30.0%
GAAGGAGAGAAATGTTACAT+AGG - tig0024668:29105-29124 None:intergenic 35.0%
!! CCGATTTTGAAAAGAGAGAA+AGG - tig0024668:29127-29146 None:intergenic 35.0%
ATCAGGGTTTGGATTTATGT+TGG - tig0024668:29173-29192 None:intergenic 35.0%
TTTGTGAATCGGAGAAGAAA+GGG - tig0024668:29275-29294 None:intergenic 35.0%
! TTTTGTGAATCGGAGAAGAA+AGG - tig0024668:29276-29295 None:intergenic 35.0%
ACAAAATCTCCTTCAAGATG+CGG + tig0024668:29290-29309 Msa1419060:five_prime_UTR 35.0%
AATTGAAAACCGCATCTTGA+AGG - tig0024668:29302-29321 None:intergenic 35.0%
! TCAAGATGCGGTTTTCAATT+CGG + tig0024668:29302-29321 Msa1419060:five_prime_UTR 35.0%
! TGAAATTCTTCGAAGAGTGT+TGG - tig0024668:29495-29514 None:intergenic 35.0%
!!! TTGGTGATTGATGGGTTTTT+AGG + tig0024668:29771-29790 Msa1419060:intron 35.0%
!!! TGGTGATTGATGGGTTTTTA+GGG + tig0024668:29772-29791 Msa1419060:intron 35.0%
!! GGATATGGATGTTTGTTGTT+TGG + tig0024668:29813-29832 Msa1419060:five_prime_UTR 35.0%
AACAAACATTACCGATGTGA+AGG - tig0024668:29945-29964 None:intergenic 35.0%
!! AATAGTTGCTGATTCACTTC+TGG + tig0024668:30079-30098 Msa1419060:intron 35.0%
!! ATTCAATCCACTTTTCTGGA+AGG - tig0024668:30254-30273 None:intergenic 35.0%
!! TCCAGAAAAGTGGATTGAAT+TGG + tig0024668:30254-30273 Msa1419060:intron 35.0%
!! CCAGAAAAGTGGATTGAATT+GGG + tig0024668:30255-30274 Msa1419060:intron 35.0%
! AATGCCGTTACAGCTTTTAA+GGG + tig0024668:30346-30365 Msa1419060:CDS 35.0%
!! CAGCTTTTAAGGGAAAACAT+TGG + tig0024668:30356-30375 Msa1419060:CDS 35.0%
TTTGTCAAATGCATTAGCTC+AGG - tig0024668:30403-30422 None:intergenic 35.0%
GTCCAATCTTTCTATTGAAG+TGG + tig0024668:30523-30542 Msa1419060:three_prime_UTR 35.0%
! TGCCACTTCAATAGAAAGAT+TGG - tig0024668:30528-30547 None:intergenic 35.0%
ATCTTTCTATTGAAGTGGCA+AGG + tig0024668:30528-30547 Msa1419060:three_prime_UTR 35.0%
GAAGGAGAGAAATGTTACAT+AGG - tig0024668:29105-29124 None:intergenic 35.0%
!! CCGATTTTGAAAAGAGAGAA+AGG - tig0024668:29127-29146 None:intergenic 35.0%
ATCAGGGTTTGGATTTATGT+TGG - tig0024668:29173-29192 None:intergenic 35.0%
TTTGTGAATCGGAGAAGAAA+GGG - tig0024668:29275-29294 None:intergenic 35.0%
! TTTTGTGAATCGGAGAAGAA+AGG - tig0024668:29276-29295 None:intergenic 35.0%
ACAAAATCTCCTTCAAGATG+CGG + tig0024668:29290-29309 Msa1419060:five_prime_UTR 35.0%
AATTGAAAACCGCATCTTGA+AGG - tig0024668:29302-29321 None:intergenic 35.0%
! TCAAGATGCGGTTTTCAATT+CGG + tig0024668:29302-29321 Msa1419060:five_prime_UTR 35.0%
! TGAAATTCTTCGAAGAGTGT+TGG - tig0024668:29495-29514 None:intergenic 35.0%
!!! TTGGTGATTGATGGGTTTTT+AGG + tig0024668:29771-29790 Msa1419060:intron 35.0%
!!! TGGTGATTGATGGGTTTTTA+GGG + tig0024668:29772-29791 Msa1419060:intron 35.0%
!! GGATATGGATGTTTGTTGTT+TGG + tig0024668:29813-29832 Msa1419060:five_prime_UTR 35.0%
AACAAACATTACCGATGTGA+AGG - tig0024668:29945-29964 None:intergenic 35.0%
!! AATAGTTGCTGATTCACTTC+TGG + tig0024668:30079-30098 Msa1419060:intron 35.0%
!! ATTCAATCCACTTTTCTGGA+AGG - tig0024668:30254-30273 None:intergenic 35.0%
!! TCCAGAAAAGTGGATTGAAT+TGG + tig0024668:30254-30273 Msa1419060:intron 35.0%
!! CCAGAAAAGTGGATTGAATT+GGG + tig0024668:30255-30274 Msa1419060:intron 35.0%
! AATGCCGTTACAGCTTTTAA+GGG + tig0024668:30346-30365 Msa1419060:CDS 35.0%
!! CAGCTTTTAAGGGAAAACAT+TGG + tig0024668:30356-30375 Msa1419060:CDS 35.0%
TTTGTCAAATGCATTAGCTC+AGG - tig0024668:30403-30422 None:intergenic 35.0%
GTCCAATCTTTCTATTGAAG+TGG + tig0024668:30523-30542 Msa1419060:three_prime_UTR 35.0%
! TGCCACTTCAATAGAAAGAT+TGG - tig0024668:30528-30547 None:intergenic 35.0%
ATCTTTCTATTGAAGTGGCA+AGG + tig0024668:30528-30547 Msa1419060:three_prime_UTR 35.0%
TGTTTGGGCTTCTCTCTAAT+TGG - tig0024668:29029-29048 None:intergenic 40.0%
! GTTTGGATTTATGTTGGTGG+AGG - tig0024668:29167-29186 None:intergenic 40.0%
AGGGTTTGGATTTATGTTGG+TGG - tig0024668:29170-29189 None:intergenic 40.0%
AGAAGGGGAAAATAGTGTGA+GGG - tig0024668:29203-29222 None:intergenic 40.0%
AAGAAGGGGAAAATAGTGTG+AGG - tig0024668:29204-29223 None:intergenic 40.0%
AGGGAAGGAAGATAAAGAAG+GGG - tig0024668:29218-29237 None:intergenic 40.0%
GAGGGAAGGAAGATAAAGAA+GGG - tig0024668:29219-29238 None:intergenic 40.0%
TGAGGGAAGGAAGATAAAGA+AGG - tig0024668:29220-29239 None:intergenic 40.0%
!! GATGGGTTTTTAGGGATTGA+AGG + tig0024668:29780-29799 Msa1419060:intron 40.0%
!! GAAGGTTTTGGTGAAGGATA+TGG + tig0024668:29798-29817 Msa1419060:five_prime_UTR 40.0%
AAACATTACCGATGTGAAGG+CGG - tig0024668:29942-29961 None:intergenic 40.0%
! TTTTCCTCCTTCCAGAAAAG+TGG + tig0024668:30244-30263 Msa1419060:intron 40.0%
!! CCCAATTCAATCCACTTTTC+TGG - tig0024668:30258-30277 None:intergenic 40.0%
! GAATGCCGTTACAGCTTTTA+AGG + tig0024668:30345-30364 Msa1419060:CDS 40.0%
ATTGAAGTGGCAAGGATGAT+TGG + tig0024668:30536-30555 Msa1419060:three_prime_UTR 40.0%
TGTTTGGGCTTCTCTCTAAT+TGG - tig0024668:29029-29048 None:intergenic 40.0%
! GTTTGGATTTATGTTGGTGG+AGG - tig0024668:29167-29186 None:intergenic 40.0%
AGGGTTTGGATTTATGTTGG+TGG - tig0024668:29170-29189 None:intergenic 40.0%
AGAAGGGGAAAATAGTGTGA+GGG - tig0024668:29203-29222 None:intergenic 40.0%
AAGAAGGGGAAAATAGTGTG+AGG - tig0024668:29204-29223 None:intergenic 40.0%
AGGGAAGGAAGATAAAGAAG+GGG - tig0024668:29218-29237 None:intergenic 40.0%
GAGGGAAGGAAGATAAAGAA+GGG - tig0024668:29219-29238 None:intergenic 40.0%
TGAGGGAAGGAAGATAAAGA+AGG - tig0024668:29220-29239 None:intergenic 40.0%
!! GATGGGTTTTTAGGGATTGA+AGG + tig0024668:29780-29799 Msa1419060:intron 40.0%
!! GAAGGTTTTGGTGAAGGATA+TGG + tig0024668:29798-29817 Msa1419060:five_prime_UTR 40.0%
AAACATTACCGATGTGAAGG+CGG - tig0024668:29942-29961 None:intergenic 40.0%
! TTTTCCTCCTTCCAGAAAAG+TGG + tig0024668:30244-30263 Msa1419060:intron 40.0%
!! CCCAATTCAATCCACTTTTC+TGG - tig0024668:30258-30277 None:intergenic 40.0%
! GAATGCCGTTACAGCTTTTA+AGG + tig0024668:30345-30364 Msa1419060:CDS 40.0%
ATTGAAGTGGCAAGGATGAT+TGG + tig0024668:30536-30555 Msa1419060:three_prime_UTR 40.0%
! TGGATTTATGTTGGTGGAGG+TGG - tig0024668:29164-29183 None:intergenic 45.0%
AGGGAGAAGATATCAGGGTT+TGG - tig0024668:29184-29203 None:intergenic 45.0%
GTGTGAGGGAGAAGATATCA+GGG - tig0024668:29189-29208 None:intergenic 45.0%
AGTGTGAGGGAGAAGATATC+AGG - tig0024668:29190-29209 None:intergenic 45.0%
! AAAGAGAAGCATGTGAGGGA+AGG - tig0024668:29233-29252 None:intergenic 45.0%
! TGGCAAAGAGAAGCATGTGA+GGG - tig0024668:29237-29256 None:intergenic 45.0%
!! GGGATTGAAGGTTTTGGTGA+AGG + tig0024668:29792-29811 Msa1419060:intron 45.0%
TCTTGCTCATCTGTGTCTGA+AGG + tig0024668:29858-29877 Msa1419060:five_prime_UTR 45.0%
GGAGGAGAACAAGTCCTATA+AGG + tig0024668:30158-30177 Msa1419060:intron 45.0%
AAGTCCTATAAGGCACGCTA+AGG + tig0024668:30168-30187 Msa1419060:CDS 45.0%
AGTCCTATAAGGCACGCTAA+GGG + tig0024668:30169-30188 Msa1419060:CDS 45.0%
!! CAATCCACTTTTCTGGAAGG+AGG - tig0024668:30251-30270 None:intergenic 45.0%
! TGGATTTATGTTGGTGGAGG+TGG - tig0024668:29164-29183 None:intergenic 45.0%
AGGGAGAAGATATCAGGGTT+TGG - tig0024668:29184-29203 None:intergenic 45.0%
GTGTGAGGGAGAAGATATCA+GGG - tig0024668:29189-29208 None:intergenic 45.0%
AGTGTGAGGGAGAAGATATC+AGG - tig0024668:29190-29209 None:intergenic 45.0%
! AAAGAGAAGCATGTGAGGGA+AGG - tig0024668:29233-29252 None:intergenic 45.0%
! TGGCAAAGAGAAGCATGTGA+GGG - tig0024668:29237-29256 None:intergenic 45.0%
!! GGGATTGAAGGTTTTGGTGA+AGG + tig0024668:29792-29811 Msa1419060:intron 45.0%
TCTTGCTCATCTGTGTCTGA+AGG + tig0024668:29858-29877 Msa1419060:five_prime_UTR 45.0%
GGAGGAGAACAAGTCCTATA+AGG + tig0024668:30158-30177 Msa1419060:intron 45.0%
AAGTCCTATAAGGCACGCTA+AGG + tig0024668:30168-30187 Msa1419060:CDS 45.0%
AGTCCTATAAGGCACGCTAA+GGG + tig0024668:30169-30188 Msa1419060:CDS 45.0%
!! CAATCCACTTTTCTGGAAGG+AGG - tig0024668:30251-30270 None:intergenic 45.0%
! GTGGCAAAGAGAAGCATGTG+AGG - tig0024668:29238-29257 None:intergenic 50.0%
AGAAAGGGAGGAGGTTCAGA+TGG - tig0024668:29260-29279 None:intergenic 50.0%
AATCGGAGAAGAAAGGGAGG+AGG - tig0024668:29269-29288 None:intergenic 50.0%
GTGAATCGGAGAAGAAAGGG+AGG - tig0024668:29272-29291 None:intergenic 50.0%
TTTGCCTCTGCACTTTGAGC+TGG + tig0024668:30813-30832 Msa1419060:three_prime_UTR 50.0%
ATGTCCAGCTCAAAGTGCAG+AGG - tig0024668:30820-30839 None:intergenic 50.0%
! GTGGCAAAGAGAAGCATGTG+AGG - tig0024668:29238-29257 None:intergenic 50.0%
AGAAAGGGAGGAGGTTCAGA+TGG - tig0024668:29260-29279 None:intergenic 50.0%
AATCGGAGAAGAAAGGGAGG+AGG - tig0024668:29269-29288 None:intergenic 50.0%
GTGAATCGGAGAAGAAAGGG+AGG - tig0024668:29272-29291 None:intergenic 50.0%
TTTGCCTCTGCACTTTGAGC+TGG + tig0024668:30813-30832 Msa1419060:three_prime_UTR 50.0%
ATGTCCAGCTCAAAGTGCAG+AGG - tig0024668:30820-30839 None:intergenic 50.0%
AAGGGAGGAGGTTCAGATGG+TGG - tig0024668:29257-29276 None:intergenic 55.0%
TCCAGGGATATGTAGCCCCA+TGG + tig0024668:29905-29924 Msa1419060:exon 55.0%
CATCCACACCGCCTTCACAT+CGG + tig0024668:29931-29950 Msa1419060:CDS 55.0%
TTACCGATGTGAAGGCGGTG+TGG - tig0024668:29937-29956 None:intergenic 55.0%
CCACCCTTAGCGTGCCTTAT+AGG - tig0024668:30175-30194 None:intergenic 55.0%
CCTATAAGGCACGCTAAGGG+TGG + tig0024668:30172-30191 Msa1419060:CDS 55.0%
TAAGGCACGCTAAGGGTGGT+TGG + tig0024668:30176-30195 Msa1419060:CDS 55.0%
AAGGGAGGAGGTTCAGATGG+TGG - tig0024668:29257-29276 None:intergenic 55.0%
TCCAGGGATATGTAGCCCCA+TGG + tig0024668:29905-29924 Msa1419060:exon 55.0%
CATCCACACCGCCTTCACAT+CGG + tig0024668:29931-29950 Msa1419060:CDS 55.0%
TTACCGATGTGAAGGCGGTG+TGG - tig0024668:29937-29956 None:intergenic 55.0%
CCACCCTTAGCGTGCCTTAT+AGG - tig0024668:30175-30194 None:intergenic 55.0%
CCTATAAGGCACGCTAAGGG+TGG + tig0024668:30172-30191 Msa1419060:CDS 55.0%
TAAGGCACGCTAAGGGTGGT+TGG + tig0024668:30176-30195 Msa1419060:CDS 55.0%
GCCATGGGGCTACATATCCC+TGG - tig0024668:29909-29928 None:intergenic 60.0%
GCGGTGTGGATGTAGCCATG+GGG - tig0024668:29923-29942 None:intergenic 60.0%
GGCGGTGTGGATGTAGCCAT+GGG - tig0024668:29924-29943 None:intergenic 60.0%
AGGCGGTGTGGATGTAGCCA+TGG - tig0024668:29925-29944 None:intergenic 60.0%
GGGTGGTTGGACAGCAGAAG+AGG + tig0024668:30189-30208 Msa1419060:CDS 60.0%
GCCATGGGGCTACATATCCC+TGG - tig0024668:29909-29928 None:intergenic 60.0%
GCGGTGTGGATGTAGCCATG+GGG - tig0024668:29923-29942 None:intergenic 60.0%
GGCGGTGTGGATGTAGCCAT+GGG - tig0024668:29924-29943 None:intergenic 60.0%
AGGCGGTGTGGATGTAGCCA+TGG - tig0024668:29925-29944 None:intergenic 60.0%
GGGTGGTTGGACAGCAGAAG+AGG + tig0024668:30189-30208 Msa1419060:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0024668 gene 29006 30935 29006 ID=Msa1419060;Name=Msa1419060
tig0024668 mRNA 29006 30935 29006 ID=Msa1419060-mRNA-1;Parent=Msa1419060;Name=Msa1419060-mRNA-1;_AED=0.32;_eAED=0.32;_QI=496|1|1|1|0.66|0.5|4|509|58
tig0024668 exon 29006 29370 29006 ID=Msa1419060-mRNA-1:exon:748;Parent=Msa1419060-mRNA-1
tig0024668 exon 29793 29952 29793 ID=Msa1419060-mRNA-1:exon:749;Parent=Msa1419060-mRNA-1
tig0024668 exon 30159 30210 30159 ID=Msa1419060-mRNA-1:exon:750;Parent=Msa1419060-mRNA-1
tig0024668 exon 30331 30935 30331 ID=Msa1419060-mRNA-1:exon:751;Parent=Msa1419060-mRNA-1
tig0024668 five_prime_UTR 29006 29370 29006 ID=Msa1419060-mRNA-1:five_prime_utr;Parent=Msa1419060-mRNA-1
tig0024668 five_prime_UTR 29793 29923 29793 ID=Msa1419060-mRNA-1:five_prime_utr;Parent=Msa1419060-mRNA-1
tig0024668 CDS 29924 29952 29924 ID=Msa1419060-mRNA-1:cds;Parent=Msa1419060-mRNA-1
tig0024668 CDS 30159 30210 30159 ID=Msa1419060-mRNA-1:cds;Parent=Msa1419060-mRNA-1
tig0024668 CDS 30331 30426 30331 ID=Msa1419060-mRNA-1:cds;Parent=Msa1419060-mRNA-1
tig0024668 three_prime_UTR 30427 30935 30427 ID=Msa1419060-mRNA-1:three_prime_utr;Parent=Msa1419060-mRNA-1
Gene Sequence

>Msa1419060

ATGGCTACATCCACACCGCCTTCACATCGGAGGAGAACAAGTCCTATAAGGCACGCTAAGGGTGGTTGGACAGCAGAAGAGGATGAGACATTAAGGAATGCCGTTACAGCTTTTAAGGGAAAACATTGGAAGAAAATAGGTAACTATTTGCCTGAGCTAATGCATTTGACAAATTAA
Protein sequence

>Msa1419060

MATSTPPSHRRRTSPIRHAKGGWTAEEDETLRNAVTAFKGKHWKKIGNYLPELMHLTN*