AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1218700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1218700 MtrunA17_Chr7g0238631 88.889 45 5 0 2 46 43 87 1.92e-21 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1218700 AT2G13960.1 72.500 40 10 1 8 46 62 101 1.58e-12 58.9
Msa1218700 AT3G09370.2 67.442 43 11 1 7 46 67 109 1.76e-12 61.2
Msa1218700 AT3G09370.3 67.442 43 11 1 7 46 75 117 1.77e-12 61.2
Msa1218700 AT3G09370.1 67.442 43 11 1 7 46 62 104 1.85e-12 61.2
Msa1218700 AT2G13960.2 70.270 37 11 0 10 46 30 66 2.28e-12 57.8
Msa1218700 AT5G02320.3 70.270 37 11 0 10 46 5 41 1.12e-11 58.9
Msa1218700 AT5G02320.1 70.270 37 11 0 10 46 65 101 5.04e-11 57.0
Msa1218700 AT5G02320.2 70.270 37 11 0 10 46 65 101 5.04e-11 57.0

Find 51 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTAATTGGTCAATTATT+TGG 0.166929 8_1:+81335001 None:intergenic
CCACCCTTAGCGTGCCTTAT+AGG 0.202151 8_1:+81333843 None:intergenic
TATTGTAGGATGAGACATTA+AGG 0.293653 8_1:-81333692 Msa1218700:intron
TGGATTTATGTTGGTGGAGG+TGG 0.309935 8_1:+81334854 None:intergenic
GGAAAACATTGGAAGAAAAT+AGG 0.311225 8_1:-81333648 Msa1218700:intron
ATCATATTATTAGATGTAAT+TGG 0.326571 8_1:-81332740 Msa1218700:intron
TGTTTGGGCTTCTCTCTAAT+TGG 0.328403 8_1:+81334989 None:intergenic
CTCATCTTCAAATCATATCT+AGG 0.331174 8_1:-81334667 Msa1218700:five_prime_UTR
AGTGTGAGGGAGAAGATATC+AGG 0.349806 8_1:+81334828 None:intergenic
GGATATGGATGTTTGTTGTT+TGG 0.389621 8_1:-81334202 Msa1218700:five_prime_UTR
GGAGGAGAACAAGTCCTATA+AGG 0.409948 8_1:-81333857 Msa1218700:CDS
AGGGAGAAGATATCAGGGTT+TGG 0.428253 8_1:+81334834 None:intergenic
AGGGTTTGGATTTATGTTGG+TGG 0.431049 8_1:+81334848 None:intergenic
AATTGAAAACCGCATCTTGA+AGG 0.449911 8_1:+81334716 None:intergenic
AGTATAATTCAAAAGTCTCC+AGG 0.458256 8_1:-81334127 Msa1218700:five_prime_UTR
ATCAGGGTTTGGATTTATGT+TGG 0.464167 8_1:+81334845 None:intergenic
TTTGTGAATCGGAGAAGAAA+GGG 0.464883 8_1:+81334743 None:intergenic
AGAAAGGGAGGAGGTTCAGA+TGG 0.474386 8_1:+81334758 None:intergenic
AGGCGGTGTGGATGTAGCCA+TGG 0.485223 8_1:+81334093 None:intergenic
GTTTGGATTTATGTTGGTGG+AGG 0.500329 8_1:+81334851 None:intergenic
GGCGGTGTGGATGTAGCCAT+GGG 0.512625 8_1:+81334094 None:intergenic
GAAGGAGAGAAATGTTACAT+AGG 0.517797 8_1:+81334913 None:intergenic
GAGGGAAGGAAGATAAAGAA+GGG 0.520886 8_1:+81334799 None:intergenic
GAAATGTTACATAGGAAATG+TGG 0.521689 8_1:+81334921 None:intergenic
GTGTGAGGGAGAAGATATCA+GGG 0.524168 8_1:+81334829 None:intergenic
TGAGGGAAGGAAGATAAAGA+AGG 0.525432 8_1:+81334798 None:intergenic
AAGTCCTATAAGGCACGCTA+AGG 0.528953 8_1:-81333847 Msa1218700:CDS
TCATCTTCAAATCATATCTA+GGG 0.535900 8_1:-81334666 Msa1218700:intron
GCCATGGGGCTACATATCCC+TGG 0.552664 8_1:+81334109 None:intergenic
TCTTGCTCATCTGTGTCTGA+AGG 0.559484 8_1:-81334157 Msa1218700:five_prime_UTR
ACAAAATCTCCTTCAAGATG+CGG 0.561408 8_1:-81334725 Msa1218700:five_prime_UTR
GTGAATCGGAGAAGAAAGGG+AGG 0.561423 8_1:+81334746 None:intergenic
TAAGGCACGCTAAGGGTGGT+TGG 0.562887 8_1:-81333839 Msa1218700:CDS
AAAGAGAAGCATGTGAGGGA+AGG 0.591322 8_1:+81334785 None:intergenic
AACAAACATTACCGATGTGA+AGG 0.591884 8_1:+81334073 None:intergenic
AGAAGGGGAAAATAGTGTGA+GGG 0.595581 8_1:+81334815 None:intergenic
GTGGCAAAGAGAAGCATGTG+AGG 0.601561 8_1:+81334780 None:intergenic
GGGTGGTTGGACAGCAGAAG+AGG 0.605706 8_1:-81333826 Msa1218700:intron
AGGGAAGGAAGATAAAGAAG+GGG 0.607295 8_1:+81334800 None:intergenic
CCTATAAGGCACGCTAAGGG+TGG 0.618742 8_1:-81333843 Msa1218700:CDS
AGTCCTATAAGGCACGCTAA+GGG 0.620051 8_1:-81333846 Msa1218700:CDS
AATCGGAGAAGAAAGGGAGG+AGG 0.620302 8_1:+81334749 None:intergenic
AAGAAGGGGAAAATAGTGTG+AGG 0.621252 8_1:+81334814 None:intergenic
AAGGGAGGAGGTTCAGATGG+TGG 0.627661 8_1:+81334761 None:intergenic
GTATAATTCAAAAGTCTCCA+GGG 0.641290 8_1:-81334126 Msa1218700:five_prime_UTR
TCCAGGGATATGTAGCCCCA+TGG 0.641883 8_1:-81334110 Msa1218700:exon
CATCCACACCGCCTTCACAT+CGG 0.670345 8_1:-81334084 Msa1218700:intron
TGGCAAAGAGAAGCATGTGA+GGG 0.676512 8_1:+81334781 None:intergenic
AAACATTACCGATGTGAAGG+CGG 0.687678 8_1:+81334076 None:intergenic
TTACCGATGTGAAGGCGGTG+TGG 0.693836 8_1:+81334081 None:intergenic
GCGGTGTGGATGTAGCCATG+GGG 0.784527 8_1:+81334095 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTAGTAATTAAAGA+TGG + chr8_1:81333082-81333101 None:intergenic 10.0%
!!! ATTTTTTTTTTTTTATACTG+AGG - chr8_1:81333368-81333387 Msa1218700:intron 10.0%
!! AAATAATTAGTAATTAAAGA+TGG + chr8_1:81333082-81333101 None:intergenic 10.0%
!!! ATTTTTTTTTTTTTATACTG+AGG - chr8_1:81333368-81333387 Msa1218700:intron 10.0%
!! AATATAAATGCATTGAATAA+AGG + chr8_1:81333032-81333051 None:intergenic 15.0%
!!! TTTTTTTATTGGTTTTGTTT+TGG - chr8_1:81333242-81333261 Msa1218700:intron 15.0%
!!! GATAAAGATTTTGTTTTTTT+GGG - chr8_1:81333264-81333283 Msa1218700:intron 15.0%
!!! ACAAATTAAAAGTGAATTTT+TGG + chr8_1:81334773-81334792 None:intergenic 15.0%
!! ATCATATTATTAGATGTAAT+TGG - chr8_1:81334918-81334937 Msa1218700:five_prime_UTR 15.0%
!! AATATAAATGCATTGAATAA+AGG + chr8_1:81333032-81333051 None:intergenic 15.0%
!!! TTTTTTTATTGGTTTTGTTT+TGG - chr8_1:81333242-81333261 Msa1218700:intron 15.0%
!!! GATAAAGATTTTGTTTTTTT+GGG - chr8_1:81333264-81333283 Msa1218700:intron 15.0%
!!! ACAAATTAAAAGTGAATTTT+TGG + chr8_1:81334773-81334792 None:intergenic 15.0%
!! ATCATATTATTAGATGTAAT+TGG - chr8_1:81334918-81334937 Msa1218700:five_prime_UTR 15.0%
!!! GTCAAAAAACGTTTTTTTAT+TGG - chr8_1:81333231-81333250 Msa1218700:intron 20.0%
!!! GGATAAAGATTTTGTTTTTT+TGG - chr8_1:81333263-81333282 Msa1218700:intron 20.0%
!!! TTTTTTTGGGAAAAAATTGA+AGG - chr8_1:81333277-81333296 Msa1218700:intron 20.0%
!!! TTTTTTTTTTATACTGAGGA+TGG - chr8_1:81333372-81333391 Msa1218700:intron 20.0%
!!! TTTTTAGGGTTTTTTATGTT+TGG - chr8_1:81333395-81333414 Msa1218700:intron 20.0%
!!! TGTTATGTTATTTACGTTTT+TGG - chr8_1:81333604-81333623 Msa1218700:intron 20.0%
!!! CATTTTAATTAGAAAGATTG+AGG - chr8_1:81334243-81334262 Msa1218700:intron 20.0%
!!! ATCATTCAACTTGATTTTTA+GGG + chr8_1:81334685-81334704 None:intergenic 20.0%
!! ATTCATTTCTCATATATACT+TGG - chr8_1:81334886-81334905 Msa1218700:five_prime_UTR 20.0%
!!! GTCAAAAAACGTTTTTTTAT+TGG - chr8_1:81333231-81333250 Msa1218700:intron 20.0%
!!! GGATAAAGATTTTGTTTTTT+TGG - chr8_1:81333263-81333282 Msa1218700:intron 20.0%
!!! TTTTTTTGGGAAAAAATTGA+AGG - chr8_1:81333277-81333296 Msa1218700:intron 20.0%
!!! TTTTTTTTTTATACTGAGGA+TGG - chr8_1:81333372-81333391 Msa1218700:intron 20.0%
!!! TTTTTAGGGTTTTTTATGTT+TGG - chr8_1:81333395-81333414 Msa1218700:intron 20.0%
!!! TGTTATGTTATTTACGTTTT+TGG - chr8_1:81333604-81333623 Msa1218700:intron 20.0%
!!! CATTTTAATTAGAAAGATTG+AGG - chr8_1:81334243-81334262 Msa1218700:intron 20.0%
!!! ATCATTCAACTTGATTTTTA+GGG + chr8_1:81334685-81334704 None:intergenic 20.0%
!! ATTCATTTCTCATATATACT+TGG - chr8_1:81334886-81334905 Msa1218700:five_prime_UTR 20.0%
! TCTCTAATTGGTCAATTATT+TGG + chr8_1:81332660-81332679 None:intergenic 25.0%
! TCATCTTCAAATCATATCTA+GGG - chr8_1:81332992-81333011 Msa1218700:intron 25.0%
!! TTGATTGAGTTTTTGTGTTT+TGG - chr8_1:81333185-81333204 Msa1218700:intron 25.0%
!!! AGTTTTTGTGTTTTGGATAA+AGG - chr8_1:81333192-81333211 Msa1218700:intron 25.0%
!!! GTTTTTGTGTTTTGGATAAA+GGG - chr8_1:81333193-81333212 Msa1218700:intron 25.0%
!!! TTTTTGTGTTTTGGATAAAG+GGG - chr8_1:81333194-81333213 Msa1218700:intron 25.0%
!!! TGAAGGTTTTGATTTGATTA+TGG - chr8_1:81333294-81333313 Msa1218700:intron 25.0%
!!! TTTTTATGTTTGGTGATTGA+TGG - chr8_1:81333405-81333424 Msa1218700:intron 25.0%
!!! TTTTATGTTTGGTGATTGAT+GGG - chr8_1:81333406-81333425 Msa1218700:intron 25.0%
! ATTGAAGAACACAATAAGAA+CGG + chr8_1:81333945-81333964 None:intergenic 25.0%
! TGTGTTCTTCAATTTATTGT+AGG - chr8_1:81333952-81333971 Msa1218700:intron 25.0%
! GATTATGAAGTAAAGACAAA+AGG + chr8_1:81334209-81334228 None:intergenic 25.0%
! GTAATGTTTCATACATTTCA+AGG + chr8_1:81334358-81334377 None:intergenic 25.0%
!!! GTGTTTTTTGTTGATTTTGT+TGG - chr8_1:81334425-81334444 Msa1218700:intron 25.0%
!! GATCATTCAACTTGATTTTT+AGG + chr8_1:81334686-81334705 None:intergenic 25.0%
!!! AAATCAAGTTGAATGATCTA+TGG - chr8_1:81334688-81334707 Msa1218700:five_prime_UTR 25.0%
!!! AATCAAGTTGAATGATCTAT+GGG - chr8_1:81334689-81334708 Msa1218700:five_prime_UTR 25.0%
! GAAAAATGGAAAAATCAGAA+AGG - chr8_1:81334977-81334996 Msa1218700:five_prime_UTR 25.0%
! TCTCTAATTGGTCAATTATT+TGG + chr8_1:81332660-81332679 None:intergenic 25.0%
! TCATCTTCAAATCATATCTA+GGG - chr8_1:81332992-81333011 Msa1218700:intron 25.0%
!! TTGATTGAGTTTTTGTGTTT+TGG - chr8_1:81333185-81333204 Msa1218700:intron 25.0%
!!! AGTTTTTGTGTTTTGGATAA+AGG - chr8_1:81333192-81333211 Msa1218700:intron 25.0%
!!! GTTTTTGTGTTTTGGATAAA+GGG - chr8_1:81333193-81333212 Msa1218700:intron 25.0%
!!! TTTTTGTGTTTTGGATAAAG+GGG - chr8_1:81333194-81333213 Msa1218700:intron 25.0%
!!! TGAAGGTTTTGATTTGATTA+TGG - chr8_1:81333294-81333313 Msa1218700:intron 25.0%
!!! TTTTTATGTTTGGTGATTGA+TGG - chr8_1:81333405-81333424 Msa1218700:intron 25.0%
!!! TTTTATGTTTGGTGATTGAT+GGG - chr8_1:81333406-81333425 Msa1218700:intron 25.0%
! ATTGAAGAACACAATAAGAA+CGG + chr8_1:81333945-81333964 None:intergenic 25.0%
! TGTGTTCTTCAATTTATTGT+AGG - chr8_1:81333952-81333971 Msa1218700:intron 25.0%
! GATTATGAAGTAAAGACAAA+AGG + chr8_1:81334209-81334228 None:intergenic 25.0%
! GTAATGTTTCATACATTTCA+AGG + chr8_1:81334358-81334377 None:intergenic 25.0%
!!! GTGTTTTTTGTTGATTTTGT+TGG - chr8_1:81334425-81334444 Msa1218700:intron 25.0%
!! GATCATTCAACTTGATTTTT+AGG + chr8_1:81334686-81334705 None:intergenic 25.0%
!!! AAATCAAGTTGAATGATCTA+TGG - chr8_1:81334688-81334707 Msa1218700:five_prime_UTR 25.0%
!!! AATCAAGTTGAATGATCTAT+GGG - chr8_1:81334689-81334708 Msa1218700:five_prime_UTR 25.0%
! GAAAAATGGAAAAATCAGAA+AGG - chr8_1:81334977-81334996 Msa1218700:five_prime_UTR 25.0%
! TTTTGCTTGTAGTCATGTTT+GGG + chr8_1:81332687-81332706 None:intergenic 30.0%
! TTTTTGCTTGTAGTCATGTT+TGG + chr8_1:81332688-81332707 None:intergenic 30.0%
GAAATGTTACATAGGAAATG+TGG + chr8_1:81332740-81332759 None:intergenic 30.0%
!! TTTTGAAAAGAGAGAAAGGA+AGG + chr8_1:81332766-81332785 None:intergenic 30.0%
! CCTTTCTCTCTTTTCAAAAT+CGG - chr8_1:81332767-81332786 Msa1218700:intron 30.0%
! TTGAAGGAGATTTTGTGAAT+CGG + chr8_1:81332929-81332948 None:intergenic 30.0%
CTCATCTTCAAATCATATCT+AGG - chr8_1:81332991-81333010 Msa1218700:intron 30.0%
TCGAAGAATTTCAAGATTTC+TGG - chr8_1:81333145-81333164 Msa1218700:intron 30.0%
!!! TAAAGGGGTTTGATTTGTTT+TGG - chr8_1:81333209-81333228 Msa1218700:intron 30.0%
!!! TGATTATGGATTTTGAGATC+TGG - chr8_1:81333308-81333327 Msa1218700:intron 30.0%
!!! GATTATGGATTTTGAGATCT+GGG - chr8_1:81333309-81333328 Msa1218700:intron 30.0%
! TGAGTAAAAGTGTGAAAGTT+TGG - chr8_1:81333345-81333364 Msa1218700:intron 30.0%
! TTATACTGAGGATGGTTTTT+AGG - chr8_1:81333380-81333399 Msa1218700:intron 30.0%
! TATACTGAGGATGGTTTTTA+GGG - chr8_1:81333381-81333400 Msa1218700:intron 30.0%
!! TTTTTAGGGATTGAAGGTTT+TGG - chr8_1:81333429-81333448 Msa1218700:intron 30.0%
AGTATAATTCAAAAGTCTCC+AGG - chr8_1:81333531-81333550 Msa1218700:intron 30.0%
GTATAATTCAAAAGTCTCCA+GGG - chr8_1:81333532-81333551 Msa1218700:intron 30.0%
TTTGGTTGCAGTTGTAATTA+TGG - chr8_1:81333622-81333641 Msa1218700:intron 30.0%
! TTTTATGCCAAATCTGCTAT+TGG - chr8_1:81333681-81333700 Msa1218700:CDS 30.0%
AATTACACCAATAGCAGATT+TGG + chr8_1:81333691-81333710 None:intergenic 30.0%
ACCATATGATATGTTTGTAC+AGG - chr8_1:81333780-81333799 Msa1218700:intron 30.0%
TCCTGTACAAACATATCATA+TGG + chr8_1:81333784-81333803 None:intergenic 30.0%
ATATGATATGTTTGTACAGG+AGG - chr8_1:81333783-81333802 Msa1218700:intron 30.0%
AGAGGTTCATATTTGCTTAT+TGG - chr8_1:81333850-81333869 Msa1218700:CDS 30.0%
TATTGTAGGATGAGACATTA+AGG - chr8_1:81333966-81333985 Msa1218700:intron 30.0%
! TTTTCCCTTAAAAGCTGTAA+CGG + chr8_1:81333996-81334015 None:intergenic 30.0%
GGAAAACATTGGAAGAAAAT+AGG - chr8_1:81334010-81334029 Msa1218700:intron 30.0%
!!! TTGTTGATTTTGTTGGCTAT+TGG - chr8_1:81334432-81334451 Msa1218700:intron 30.0%
ACAAACAAAATCAATAGCAG+AGG + chr8_1:81334490-81334509 None:intergenic 30.0%
TTGTAATCATGTCTCATAAC+TGG - chr8_1:81334506-81334525 Msa1218700:intron 30.0%
TATCATAAATAGACATGCCA+TGG - chr8_1:81334615-81334634 Msa1218700:intron 30.0%
GTCATGAAAACTGAGAAAAA+TGG - chr8_1:81334963-81334982 Msa1218700:five_prime_UTR 30.0%
! TTTTGCTTGTAGTCATGTTT+GGG + chr8_1:81332687-81332706 None:intergenic 30.0%
! TTTTTGCTTGTAGTCATGTT+TGG + chr8_1:81332688-81332707 None:intergenic 30.0%
GAAATGTTACATAGGAAATG+TGG + chr8_1:81332740-81332759 None:intergenic 30.0%
!! TTTTGAAAAGAGAGAAAGGA+AGG + chr8_1:81332766-81332785 None:intergenic 30.0%
! CCTTTCTCTCTTTTCAAAAT+CGG - chr8_1:81332767-81332786 Msa1218700:intron 30.0%
! TTGAAGGAGATTTTGTGAAT+CGG + chr8_1:81332929-81332948 None:intergenic 30.0%
CTCATCTTCAAATCATATCT+AGG - chr8_1:81332991-81333010 Msa1218700:intron 30.0%
TCGAAGAATTTCAAGATTTC+TGG - chr8_1:81333145-81333164 Msa1218700:intron 30.0%
!!! TAAAGGGGTTTGATTTGTTT+TGG - chr8_1:81333209-81333228 Msa1218700:intron 30.0%
!!! TGATTATGGATTTTGAGATC+TGG - chr8_1:81333308-81333327 Msa1218700:intron 30.0%
!!! GATTATGGATTTTGAGATCT+GGG - chr8_1:81333309-81333328 Msa1218700:intron 30.0%
! TGAGTAAAAGTGTGAAAGTT+TGG - chr8_1:81333345-81333364 Msa1218700:intron 30.0%
! TTATACTGAGGATGGTTTTT+AGG - chr8_1:81333380-81333399 Msa1218700:intron 30.0%
! TATACTGAGGATGGTTTTTA+GGG - chr8_1:81333381-81333400 Msa1218700:intron 30.0%
!! TTTTTAGGGATTGAAGGTTT+TGG - chr8_1:81333429-81333448 Msa1218700:intron 30.0%
AGTATAATTCAAAAGTCTCC+AGG - chr8_1:81333531-81333550 Msa1218700:intron 30.0%
GTATAATTCAAAAGTCTCCA+GGG - chr8_1:81333532-81333551 Msa1218700:intron 30.0%
TTTGGTTGCAGTTGTAATTA+TGG - chr8_1:81333622-81333641 Msa1218700:intron 30.0%
! TTTTATGCCAAATCTGCTAT+TGG - chr8_1:81333681-81333700 Msa1218700:CDS 30.0%
AATTACACCAATAGCAGATT+TGG + chr8_1:81333691-81333710 None:intergenic 30.0%
ACCATATGATATGTTTGTAC+AGG - chr8_1:81333780-81333799 Msa1218700:intron 30.0%
TCCTGTACAAACATATCATA+TGG + chr8_1:81333784-81333803 None:intergenic 30.0%
ATATGATATGTTTGTACAGG+AGG - chr8_1:81333783-81333802 Msa1218700:intron 30.0%
AGAGGTTCATATTTGCTTAT+TGG - chr8_1:81333850-81333869 Msa1218700:CDS 30.0%
TATTGTAGGATGAGACATTA+AGG - chr8_1:81333966-81333985 Msa1218700:intron 30.0%
! TTTTCCCTTAAAAGCTGTAA+CGG + chr8_1:81333996-81334015 None:intergenic 30.0%
GGAAAACATTGGAAGAAAAT+AGG - chr8_1:81334010-81334029 Msa1218700:intron 30.0%
!!! TTGTTGATTTTGTTGGCTAT+TGG - chr8_1:81334432-81334451 Msa1218700:intron 30.0%
ACAAACAAAATCAATAGCAG+AGG + chr8_1:81334490-81334509 None:intergenic 30.0%
TTGTAATCATGTCTCATAAC+TGG - chr8_1:81334506-81334525 Msa1218700:intron 30.0%
TATCATAAATAGACATGCCA+TGG - chr8_1:81334615-81334634 Msa1218700:intron 30.0%
GTCATGAAAACTGAGAAAAA+TGG - chr8_1:81334963-81334982 Msa1218700:five_prime_UTR 30.0%
GAAGGAGAGAAATGTTACAT+AGG + chr8_1:81332748-81332767 None:intergenic 35.0%
!! CCGATTTTGAAAAGAGAGAA+AGG + chr8_1:81332770-81332789 None:intergenic 35.0%
ATCAGGGTTTGGATTTATGT+TGG + chr8_1:81332816-81332835 None:intergenic 35.0%
TTTGTGAATCGGAGAAGAAA+GGG + chr8_1:81332918-81332937 None:intergenic 35.0%
! TTTTGTGAATCGGAGAAGAA+AGG + chr8_1:81332919-81332938 None:intergenic 35.0%
ACAAAATCTCCTTCAAGATG+CGG - chr8_1:81332933-81332952 Msa1218700:intron 35.0%
AATTGAAAACCGCATCTTGA+AGG + chr8_1:81332945-81332964 None:intergenic 35.0%
! TCAAGATGCGGTTTTCAATT+CGG - chr8_1:81332945-81332964 Msa1218700:intron 35.0%
! TGAAATTCTTCGAAGAGTGT+TGG + chr8_1:81333138-81333157 None:intergenic 35.0%
!!! TTGGTGATTGATGGGTTTTT+AGG - chr8_1:81333414-81333433 Msa1218700:intron 35.0%
!!! TGGTGATTGATGGGTTTTTA+GGG - chr8_1:81333415-81333434 Msa1218700:intron 35.0%
!! GGATATGGATGTTTGTTGTT+TGG - chr8_1:81333456-81333475 Msa1218700:intron 35.0%
AACAAACATTACCGATGTGA+AGG + chr8_1:81333588-81333607 None:intergenic 35.0%
!! AATAGTTGCTGATTCACTTC+TGG - chr8_1:81333722-81333741 Msa1218700:intron 35.0%
!! ATTCAATCCACTTTTCTGGA+AGG + chr8_1:81333897-81333916 None:intergenic 35.0%
!! TCCAGAAAAGTGGATTGAAT+TGG - chr8_1:81333897-81333916 Msa1218700:intron 35.0%
!! CCAGAAAAGTGGATTGAATT+GGG - chr8_1:81333898-81333917 Msa1218700:intron 35.0%
! AATGCCGTTACAGCTTTTAA+GGG - chr8_1:81333989-81334008 Msa1218700:intron 35.0%
!! CAGCTTTTAAGGGAAAACAT+TGG - chr8_1:81333999-81334018 Msa1218700:intron 35.0%
TTTGTCAAATGCATTAGCTC+AGG + chr8_1:81334046-81334065 None:intergenic 35.0%
GTCCAATCTTTCTATTGAAG+TGG - chr8_1:81334166-81334185 Msa1218700:five_prime_UTR 35.0%
! TGCCACTTCAATAGAAAGAT+TGG + chr8_1:81334171-81334190 None:intergenic 35.0%
ATCTTTCTATTGAAGTGGCA+AGG - chr8_1:81334171-81334190 Msa1218700:five_prime_UTR 35.0%
!!! CAACTTGATTTTTAGGGCTT+CGG + chr8_1:81334679-81334698 None:intergenic 35.0%
TATGGGCACGTATAACTTTA+TGG - chr8_1:81334706-81334725 Msa1218700:five_prime_UTR 35.0%
ATGGGCACGTATAACTTTAT+GGG - chr8_1:81334707-81334726 Msa1218700:five_prime_UTR 35.0%
!!! CTTTTAATTTGTGCCTTTCC+TGG - chr8_1:81334781-81334800 Msa1218700:five_prime_UTR 35.0%
GAAGGAGAGAAATGTTACAT+AGG + chr8_1:81332748-81332767 None:intergenic 35.0%
!! CCGATTTTGAAAAGAGAGAA+AGG + chr8_1:81332770-81332789 None:intergenic 35.0%
ATCAGGGTTTGGATTTATGT+TGG + chr8_1:81332816-81332835 None:intergenic 35.0%
TTTGTGAATCGGAGAAGAAA+GGG + chr8_1:81332918-81332937 None:intergenic 35.0%
! TTTTGTGAATCGGAGAAGAA+AGG + chr8_1:81332919-81332938 None:intergenic 35.0%
ACAAAATCTCCTTCAAGATG+CGG - chr8_1:81332933-81332952 Msa1218700:intron 35.0%
AATTGAAAACCGCATCTTGA+AGG + chr8_1:81332945-81332964 None:intergenic 35.0%
! TCAAGATGCGGTTTTCAATT+CGG - chr8_1:81332945-81332964 Msa1218700:intron 35.0%
! TGAAATTCTTCGAAGAGTGT+TGG + chr8_1:81333138-81333157 None:intergenic 35.0%
!!! TTGGTGATTGATGGGTTTTT+AGG - chr8_1:81333414-81333433 Msa1218700:intron 35.0%
!!! TGGTGATTGATGGGTTTTTA+GGG - chr8_1:81333415-81333434 Msa1218700:intron 35.0%
!! GGATATGGATGTTTGTTGTT+TGG - chr8_1:81333456-81333475 Msa1218700:intron 35.0%
AACAAACATTACCGATGTGA+AGG + chr8_1:81333588-81333607 None:intergenic 35.0%
!! AATAGTTGCTGATTCACTTC+TGG - chr8_1:81333722-81333741 Msa1218700:intron 35.0%
!! ATTCAATCCACTTTTCTGGA+AGG + chr8_1:81333897-81333916 None:intergenic 35.0%
!! TCCAGAAAAGTGGATTGAAT+TGG - chr8_1:81333897-81333916 Msa1218700:intron 35.0%
!! CCAGAAAAGTGGATTGAATT+GGG - chr8_1:81333898-81333917 Msa1218700:intron 35.0%
! AATGCCGTTACAGCTTTTAA+GGG - chr8_1:81333989-81334008 Msa1218700:intron 35.0%
!! CAGCTTTTAAGGGAAAACAT+TGG - chr8_1:81333999-81334018 Msa1218700:intron 35.0%
TTTGTCAAATGCATTAGCTC+AGG + chr8_1:81334046-81334065 None:intergenic 35.0%
GTCCAATCTTTCTATTGAAG+TGG - chr8_1:81334166-81334185 Msa1218700:five_prime_UTR 35.0%
! TGCCACTTCAATAGAAAGAT+TGG + chr8_1:81334171-81334190 None:intergenic 35.0%
ATCTTTCTATTGAAGTGGCA+AGG - chr8_1:81334171-81334190 Msa1218700:five_prime_UTR 35.0%
!!! CAACTTGATTTTTAGGGCTT+CGG + chr8_1:81334679-81334698 None:intergenic 35.0%
TATGGGCACGTATAACTTTA+TGG - chr8_1:81334706-81334725 Msa1218700:five_prime_UTR 35.0%
ATGGGCACGTATAACTTTAT+GGG - chr8_1:81334707-81334726 Msa1218700:five_prime_UTR 35.0%
!!! CTTTTAATTTGTGCCTTTCC+TGG - chr8_1:81334781-81334800 Msa1218700:five_prime_UTR 35.0%
TGTTTGGGCTTCTCTCTAAT+TGG + chr8_1:81332672-81332691 None:intergenic 40.0%
! GTTTGGATTTATGTTGGTGG+AGG + chr8_1:81332810-81332829 None:intergenic 40.0%
AGGGTTTGGATTTATGTTGG+TGG + chr8_1:81332813-81332832 None:intergenic 40.0%
AGAAGGGGAAAATAGTGTGA+GGG + chr8_1:81332846-81332865 None:intergenic 40.0%
AAGAAGGGGAAAATAGTGTG+AGG + chr8_1:81332847-81332866 None:intergenic 40.0%
AGGGAAGGAAGATAAAGAAG+GGG + chr8_1:81332861-81332880 None:intergenic 40.0%
GAGGGAAGGAAGATAAAGAA+GGG + chr8_1:81332862-81332881 None:intergenic 40.0%
TGAGGGAAGGAAGATAAAGA+AGG + chr8_1:81332863-81332882 None:intergenic 40.0%
!! GATGGGTTTTTAGGGATTGA+AGG - chr8_1:81333423-81333442 Msa1218700:intron 40.0%
!! GAAGGTTTTGGTGAAGGATA+TGG - chr8_1:81333441-81333460 Msa1218700:intron 40.0%
AAACATTACCGATGTGAAGG+CGG + chr8_1:81333585-81333604 None:intergenic 40.0%
! TTTTCCTCCTTCCAGAAAAG+TGG - chr8_1:81333887-81333906 Msa1218700:intron 40.0%
!! CCCAATTCAATCCACTTTTC+TGG + chr8_1:81333901-81333920 None:intergenic 40.0%
! GAATGCCGTTACAGCTTTTA+AGG - chr8_1:81333988-81334007 Msa1218700:intron 40.0%
ATTGAAGTGGCAAGGATGAT+TGG - chr8_1:81334179-81334198 Msa1218700:five_prime_UTR 40.0%
ATAAATAGACATGCCATGGC+AGG - chr8_1:81334619-81334638 Msa1218700:intron 40.0%
!!! TTTTAGGGCTTCGGTAGTTA+GGG + chr8_1:81334670-81334689 None:intergenic 40.0%
!!! TTTTTAGGGCTTCGGTAGTT+AGG + chr8_1:81334671-81334690 None:intergenic 40.0%
!!! TTTTGGTGTTTGAGTAGCAG+CGG + chr8_1:81334756-81334775 None:intergenic 40.0%
CATGCTAATATCACCAGGAA+AGG + chr8_1:81334797-81334816 None:intergenic 40.0%
TAGTGCATGCTAATATCACC+AGG + chr8_1:81334802-81334821 None:intergenic 40.0%
GGTGATATTAGCATGCACTA+AGG - chr8_1:81334802-81334821 Msa1218700:five_prime_UTR 40.0%
GATATTAGCATGCACTAAGG+AGG - chr8_1:81334805-81334824 Msa1218700:five_prime_UTR 40.0%
ACTAAGGAGGATCGATTACT+TGG - chr8_1:81334818-81334837 Msa1218700:five_prime_UTR 40.0%
TGGAAAAATCAGAAAGGCTG+TGG - chr8_1:81334983-81335002 Msa1218700:five_prime_UTR 40.0%
TGTTTGGGCTTCTCTCTAAT+TGG + chr8_1:81332672-81332691 None:intergenic 40.0%
! GTTTGGATTTATGTTGGTGG+AGG + chr8_1:81332810-81332829 None:intergenic 40.0%
AGGGTTTGGATTTATGTTGG+TGG + chr8_1:81332813-81332832 None:intergenic 40.0%
AGAAGGGGAAAATAGTGTGA+GGG + chr8_1:81332846-81332865 None:intergenic 40.0%
AAGAAGGGGAAAATAGTGTG+AGG + chr8_1:81332847-81332866 None:intergenic 40.0%
AGGGAAGGAAGATAAAGAAG+GGG + chr8_1:81332861-81332880 None:intergenic 40.0%
GAGGGAAGGAAGATAAAGAA+GGG + chr8_1:81332862-81332881 None:intergenic 40.0%
TGAGGGAAGGAAGATAAAGA+AGG + chr8_1:81332863-81332882 None:intergenic 40.0%
!! GATGGGTTTTTAGGGATTGA+AGG - chr8_1:81333423-81333442 Msa1218700:intron 40.0%
!! GAAGGTTTTGGTGAAGGATA+TGG - chr8_1:81333441-81333460 Msa1218700:intron 40.0%
AAACATTACCGATGTGAAGG+CGG + chr8_1:81333585-81333604 None:intergenic 40.0%
! TTTTCCTCCTTCCAGAAAAG+TGG - chr8_1:81333887-81333906 Msa1218700:intron 40.0%
!! CCCAATTCAATCCACTTTTC+TGG + chr8_1:81333901-81333920 None:intergenic 40.0%
! GAATGCCGTTACAGCTTTTA+AGG - chr8_1:81333988-81334007 Msa1218700:intron 40.0%
ATTGAAGTGGCAAGGATGAT+TGG - chr8_1:81334179-81334198 Msa1218700:five_prime_UTR 40.0%
ATAAATAGACATGCCATGGC+AGG - chr8_1:81334619-81334638 Msa1218700:intron 40.0%
!!! TTTTAGGGCTTCGGTAGTTA+GGG + chr8_1:81334670-81334689 None:intergenic 40.0%
!!! TTTTTAGGGCTTCGGTAGTT+AGG + chr8_1:81334671-81334690 None:intergenic 40.0%
!!! TTTTGGTGTTTGAGTAGCAG+CGG + chr8_1:81334756-81334775 None:intergenic 40.0%
CATGCTAATATCACCAGGAA+AGG + chr8_1:81334797-81334816 None:intergenic 40.0%
TAGTGCATGCTAATATCACC+AGG + chr8_1:81334802-81334821 None:intergenic 40.0%
GGTGATATTAGCATGCACTA+AGG - chr8_1:81334802-81334821 Msa1218700:five_prime_UTR 40.0%
GATATTAGCATGCACTAAGG+AGG - chr8_1:81334805-81334824 Msa1218700:five_prime_UTR 40.0%
ACTAAGGAGGATCGATTACT+TGG - chr8_1:81334818-81334837 Msa1218700:five_prime_UTR 40.0%
TGGAAAAATCAGAAAGGCTG+TGG - chr8_1:81334983-81335002 Msa1218700:five_prime_UTR 40.0%
! TGGATTTATGTTGGTGGAGG+TGG + chr8_1:81332807-81332826 None:intergenic 45.0%
AGGGAGAAGATATCAGGGTT+TGG + chr8_1:81332827-81332846 None:intergenic 45.0%
GTGTGAGGGAGAAGATATCA+GGG + chr8_1:81332832-81332851 None:intergenic 45.0%
AGTGTGAGGGAGAAGATATC+AGG + chr8_1:81332833-81332852 None:intergenic 45.0%
! AAAGAGAAGCATGTGAGGGA+AGG + chr8_1:81332876-81332895 None:intergenic 45.0%
! TGGCAAAGAGAAGCATGTGA+GGG + chr8_1:81332880-81332899 None:intergenic 45.0%
!! GGGATTGAAGGTTTTGGTGA+AGG - chr8_1:81333435-81333454 Msa1218700:intron 45.0%
TCTTGCTCATCTGTGTCTGA+AGG - chr8_1:81333501-81333520 Msa1218700:intron 45.0%
GGAGGAGAACAAGTCCTATA+AGG - chr8_1:81333801-81333820 Msa1218700:intron 45.0%
AAGTCCTATAAGGCACGCTA+AGG - chr8_1:81333811-81333830 Msa1218700:intron 45.0%
AGTCCTATAAGGCACGCTAA+GGG - chr8_1:81333812-81333831 Msa1218700:intron 45.0%
!! CAATCCACTTTTCTGGAAGG+AGG + chr8_1:81333894-81333913 None:intergenic 45.0%
TATGCAAAGTCAACCTGCCA+TGG + chr8_1:81334635-81334654 None:intergenic 45.0%
! TGGCAGGTTGACTTTGCATA+AGG - chr8_1:81334635-81334654 Msa1218700:intron 45.0%
! GGCAGGTTGACTTTGCATAA+GGG - chr8_1:81334636-81334655 Msa1218700:intron 45.0%
GGCACGTATAACTTTATGGG+TGG - chr8_1:81334710-81334729 Msa1218700:five_prime_UTR 45.0%
! TGGATTTATGTTGGTGGAGG+TGG + chr8_1:81332807-81332826 None:intergenic 45.0%
AGGGAGAAGATATCAGGGTT+TGG + chr8_1:81332827-81332846 None:intergenic 45.0%
GTGTGAGGGAGAAGATATCA+GGG + chr8_1:81332832-81332851 None:intergenic 45.0%
AGTGTGAGGGAGAAGATATC+AGG + chr8_1:81332833-81332852 None:intergenic 45.0%
! AAAGAGAAGCATGTGAGGGA+AGG + chr8_1:81332876-81332895 None:intergenic 45.0%
! TGGCAAAGAGAAGCATGTGA+GGG + chr8_1:81332880-81332899 None:intergenic 45.0%
!! GGGATTGAAGGTTTTGGTGA+AGG - chr8_1:81333435-81333454 Msa1218700:intron 45.0%
TCTTGCTCATCTGTGTCTGA+AGG - chr8_1:81333501-81333520 Msa1218700:intron 45.0%
GGAGGAGAACAAGTCCTATA+AGG - chr8_1:81333801-81333820 Msa1218700:intron 45.0%
AAGTCCTATAAGGCACGCTA+AGG - chr8_1:81333811-81333830 Msa1218700:intron 45.0%
AGTCCTATAAGGCACGCTAA+GGG - chr8_1:81333812-81333831 Msa1218700:intron 45.0%
!! CAATCCACTTTTCTGGAAGG+AGG + chr8_1:81333894-81333913 None:intergenic 45.0%
TATGCAAAGTCAACCTGCCA+TGG + chr8_1:81334635-81334654 None:intergenic 45.0%
! TGGCAGGTTGACTTTGCATA+AGG - chr8_1:81334635-81334654 Msa1218700:intron 45.0%
! GGCAGGTTGACTTTGCATAA+GGG - chr8_1:81334636-81334655 Msa1218700:intron 45.0%
GGCACGTATAACTTTATGGG+TGG - chr8_1:81334710-81334729 Msa1218700:five_prime_UTR 45.0%
! GTGGCAAAGAGAAGCATGTG+AGG + chr8_1:81332881-81332900 None:intergenic 50.0%
AGAAAGGGAGGAGGTTCAGA+TGG + chr8_1:81332903-81332922 None:intergenic 50.0%
AATCGGAGAAGAAAGGGAGG+AGG + chr8_1:81332912-81332931 None:intergenic 50.0%
GTGAATCGGAGAAGAAAGGG+AGG + chr8_1:81332915-81332934 None:intergenic 50.0%
TTTGCCTCTGCACTTTGAGC+TGG - chr8_1:81334456-81334475 Msa1218700:intron 50.0%
ATGTCCAGCTCAAAGTGCAG+AGG + chr8_1:81334463-81334482 None:intergenic 50.0%
! GTGGCAAAGAGAAGCATGTG+AGG + chr8_1:81332881-81332900 None:intergenic 50.0%
AGAAAGGGAGGAGGTTCAGA+TGG + chr8_1:81332903-81332922 None:intergenic 50.0%
AATCGGAGAAGAAAGGGAGG+AGG + chr8_1:81332912-81332931 None:intergenic 50.0%
GTGAATCGGAGAAGAAAGGG+AGG + chr8_1:81332915-81332934 None:intergenic 50.0%
TTTGCCTCTGCACTTTGAGC+TGG - chr8_1:81334456-81334475 Msa1218700:intron 50.0%
ATGTCCAGCTCAAAGTGCAG+AGG + chr8_1:81334463-81334482 None:intergenic 50.0%
AAGGGAGGAGGTTCAGATGG+TGG + chr8_1:81332900-81332919 None:intergenic 55.0%
TCCAGGGATATGTAGCCCCA+TGG - chr8_1:81333548-81333567 Msa1218700:intron 55.0%
CATCCACACCGCCTTCACAT+CGG - chr8_1:81333574-81333593 Msa1218700:intron 55.0%
TTACCGATGTGAAGGCGGTG+TGG + chr8_1:81333580-81333599 None:intergenic 55.0%
CCACCCTTAGCGTGCCTTAT+AGG + chr8_1:81333818-81333837 None:intergenic 55.0%
CCTATAAGGCACGCTAAGGG+TGG - chr8_1:81333815-81333834 Msa1218700:intron 55.0%
TAAGGCACGCTAAGGGTGGT+TGG - chr8_1:81333819-81333838 Msa1218700:intron 55.0%
AAGGGAGGAGGTTCAGATGG+TGG + chr8_1:81332900-81332919 None:intergenic 55.0%
TCCAGGGATATGTAGCCCCA+TGG - chr8_1:81333548-81333567 Msa1218700:intron 55.0%
CATCCACACCGCCTTCACAT+CGG - chr8_1:81333574-81333593 Msa1218700:intron 55.0%
TTACCGATGTGAAGGCGGTG+TGG + chr8_1:81333580-81333599 None:intergenic 55.0%
CCACCCTTAGCGTGCCTTAT+AGG + chr8_1:81333818-81333837 None:intergenic 55.0%
CCTATAAGGCACGCTAAGGG+TGG - chr8_1:81333815-81333834 Msa1218700:intron 55.0%
TAAGGCACGCTAAGGGTGGT+TGG - chr8_1:81333819-81333838 Msa1218700:intron 55.0%
GCCATGGGGCTACATATCCC+TGG + chr8_1:81333552-81333571 None:intergenic 60.0%
GCGGTGTGGATGTAGCCATG+GGG + chr8_1:81333566-81333585 None:intergenic 60.0%
GGCGGTGTGGATGTAGCCAT+GGG + chr8_1:81333567-81333586 None:intergenic 60.0%
AGGCGGTGTGGATGTAGCCA+TGG + chr8_1:81333568-81333587 None:intergenic 60.0%
GGGTGGTTGGACAGCAGAAG+AGG - chr8_1:81333832-81333851 Msa1218700:CDS 60.0%
GCCATGGGGCTACATATCCC+TGG + chr8_1:81333552-81333571 None:intergenic 60.0%
GCGGTGTGGATGTAGCCATG+GGG + chr8_1:81333566-81333585 None:intergenic 60.0%
GGCGGTGTGGATGTAGCCAT+GGG + chr8_1:81333567-81333586 None:intergenic 60.0%
AGGCGGTGTGGATGTAGCCA+TGG + chr8_1:81333568-81333587 None:intergenic 60.0%
GGGTGGTTGGACAGCAGAAG+AGG - chr8_1:81333832-81333851 Msa1218700:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 81332649 81335031 81332649 ID=Msa1218700;Name=Msa1218700
chr8_1 mRNA 81332649 81335031 81332649 ID=Msa1218700-mRNA-1;Parent=Msa1218700;Name=Msa1218700-mRNA-1;_AED=0.39;_eAED=0.47;_QI=496|0.75|0.8|1|0.75|0.6|5|0|79
chr8_1 exon 81332649 81332749 81332649 ID=Msa1218700-mRNA-1:exon:28806;Parent=Msa1218700-mRNA-1
chr8_1 exon 81333649 81333706 81333649 ID=Msa1218700-mRNA-1:exon:28805;Parent=Msa1218700-mRNA-1
chr8_1 exon 81333827 81333878 81333827 ID=Msa1218700-mRNA-1:exon:28804;Parent=Msa1218700-mRNA-1
chr8_1 exon 81334085 81334244 81334085 ID=Msa1218700-mRNA-1:exon:28803;Parent=Msa1218700-mRNA-1
chr8_1 exon 81334667 81335031 81334667 ID=Msa1218700-mRNA-1:exon:28802;Parent=Msa1218700-mRNA-1
chr8_1 five_prime_UTR 81334667 81335031 81334667 ID=Msa1218700-mRNA-1:five_prime_utr;Parent=Msa1218700-mRNA-1
chr8_1 five_prime_UTR 81334114 81334244 81334114 ID=Msa1218700-mRNA-1:five_prime_utr;Parent=Msa1218700-mRNA-1
chr8_1 CDS 81334085 81334113 81334085 ID=Msa1218700-mRNA-1:cds;Parent=Msa1218700-mRNA-1
chr8_1 CDS 81333827 81333878 81333827 ID=Msa1218700-mRNA-1:cds;Parent=Msa1218700-mRNA-1
chr8_1 CDS 81333649 81333706 81333649 ID=Msa1218700-mRNA-1:cds;Parent=Msa1218700-mRNA-1
chr8_1 CDS 81332649 81332749 81332649 ID=Msa1218700-mRNA-1:cds;Parent=Msa1218700-mRNA-1
Gene Sequence

>Msa1218700

ATGGCTACATCCACACCGCCTTCACATCGGAGGAGAACAAGTCCTATAAGGCACGCTAAGGGTGGTTGGACAGCAGAAGAGGATGAGACATTAAGGAATGCCGTTACAGCTTTTAAGGGAAAACATTGGAAGAAAATAGATGTAATTGGTCATGTTGTTGAGAAAGACACTGTCATGAAAACTGAGAAAAATGGAAAAATCAGAAAGGCTGTGGATGTGACAGTAGAACATTTAGAGTAA
Protein sequence

>Msa1218700

MATSTPPSHRRRTSPIRHAKGGWTAEEDETLRNAVTAFKGKHWKKIDVIGHVVEKDTVMKTEKNGKIRKAVDVTVEHLE*