AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0623140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623140 MtrunA17_Chr1g0212971 99.157 593 5 0 1 593 1 593 0.0 1201
Msa0623140 MtrunA17_Chr7g0263471 73.042 549 141 4 46 593 40 582 0.0 791
Msa0623140 MtrunA17_Chr2g0327711 46.209 554 253 9 61 593 15 544 2.55e-147 436
Msa0623140 MtrunA17_Chr4g0030551 47.126 522 237 8 58 563 16 514 2.40e-145 431
Msa0623140 MtrunA17_Chr7g0276681 45.421 546 255 7 61 593 31 546 7.71e-137 409
Msa0623140 MtrunA17_Chr1g0161231 45.436 482 246 7 127 593 52 531 5.60e-128 386
Msa0623140 MtrunA17_Chr7g0263421 42.620 542 273 7 66 593 3 520 2.95e-125 379
Msa0623140 MtrunA17_Chr8g0391711 43.659 481 256 4 127 593 59 538 6.24e-125 378
Msa0623140 MtrunA17_Chr2g0331191 22.951 610 356 14 4 567 69 610 8.96e-27 115
Msa0623140 MtrunA17_Chr2g0331191 25.403 248 184 1 96 343 1 247 1.05e-20 96.3
Msa0623140 MtrunA17_Chr3g0136651 22.707 458 298 12 138 577 8 427 3.84e-25 108
Msa0623140 MtrunA17_Chr2g0331201 28.896 308 202 5 74 367 37 341 1.77e-21 99.0
Msa0623140 MtrunA17_Chr2g0331201 26.949 449 250 15 130 522 137 563 1.68e-20 95.9
Msa0623140 MtrunA17_Chr2g0331201 26.471 238 173 2 107 343 11 247 4.28e-19 91.3
Msa0623140 MtrunA17_Chr8g0361991 25.469 373 214 10 76 445 10 321 1.60e-19 91.7
Msa0623140 MtrunA17_Chr2g0328031 31.707 205 121 4 134 322 14 215 1.99e-19 91.3
Msa0623140 MtrunA17_Chr8g0370361 26.756 299 178 6 169 452 46 318 5.30e-19 90.1
Msa0623140 MtrunA17_Chr8g0367671 23.786 412 245 12 169 543 47 426 1.21e-18 89.0
Msa0623140 MtrunA17_Chr1g0178581 24.565 460 262 15 138 556 15 430 3.83e-18 87.4
Msa0623140 MtrunA17_Chr7g0233331 24.611 321 206 6 138 445 12 309 2.26e-16 81.6
Msa0623140 MtrunA17_Chr5g0439361 25.908 413 253 13 169 562 23 401 3.14e-16 81.3
Msa0623140 MtrunA17_Chr5g0439351 27.128 376 238 11 169 533 338 688 3.67e-16 82.0
Msa0623140 MtrunA17_Chr5g0439161 24.319 477 310 14 128 562 207 674 6.51e-16 81.3
Msa0623140 MtrunA17_Chr8g0369921 23.653 427 245 13 169 556 45 429 7.85e-16 80.1
Msa0623140 MtrunA17_Chr4g0031491 30.288 208 124 7 132 322 13 216 9.13e-16 80.1
Msa0623140 MtrunA17_Chr8g0368621 24.315 292 180 6 169 445 42 307 2.74e-15 77.4
Msa0623140 MtrunA17_Chr3g0100301 24.552 391 245 14 189 542 15 392 2.94e-15 78.2
Msa0623140 MtrunA17_Chr7g0262731 27.917 240 158 6 135 361 9 246 3.95e-15 78.6
Msa0623140 MtrunA17_Chr7g0221531 23.099 355 208 9 169 488 44 368 1.99e-14 75.5
Msa0623140 MtrunA17_Chr8g0354071 23.684 342 198 10 177 480 2 318 2.79e-14 75.1
Msa0623140 MtrunA17_Chr8g0364031 25.000 336 200 10 138 453 13 316 5.79e-14 74.3
Msa0623140 MtrunA17_Chr5g0418551 33.708 178 111 5 132 306 20 193 1.02e-13 71.2
Msa0623140 MtrunA17_Chr7g0263431 71.429 49 13 1 274 321 1 49 1.26e-13 65.5
Msa0623140 MtrunA17_Chr3g0093531 23.684 342 198 10 177 480 2 318 1.90e-13 72.4
Msa0623140 MtrunA17_Chr3g0083331 24.917 301 178 9 169 445 45 321 2.71e-13 72.4
Msa0623140 MtrunA17_Chr8g0353311 21.809 376 234 9 169 509 49 399 4.61e-13 71.6
Msa0623140 MtrunA17_Chr8g0376971 26.183 317 194 9 72 352 77 389 6.24e-13 71.6
Msa0623140 MtrunA17_Chr1g0211241 29.952 207 137 5 120 322 120 322 1.10e-12 70.9
Msa0623140 MtrunA17_Chr3g0122941 28.342 187 125 3 131 309 105 290 1.56e-12 70.5
Msa0623140 MtrunA17_Chr3g0122941 27.208 283 173 9 100 355 108 384 2.92e-12 69.7
Msa0623140 MtrunA17_Chr6g0461061 27.935 247 158 7 90 323 125 364 1.63e-11 67.0
Msa0623140 MtrunA17_Chr5g0410201 25.284 352 185 11 213 544 7 300 1.71e-11 65.9
Msa0623140 MtrunA17_Chr4g0064931 30.319 188 127 4 135 322 40 223 2.05e-11 67.0
Msa0623140 MtrunA17_Chr7g0262761 27.922 154 100 3 169 312 47 199 2.36e-11 63.5
Msa0623140 MtrunA17_Chr4g0073161 30.392 204 98 6 128 294 9 205 3.05e-11 66.2
Msa0623140 MtrunA17_Chr1g0196261 28.947 228 134 8 114 317 198 421 5.70e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623140 AT3G12360.1 80.684 585 104 4 14 593 10 590 0.0 954
Msa0623140 AT3G12360.2 80.895 581 102 4 18 593 2 578 0.0 950
Msa0623140 AT3G09550.1 71.502 586 148 3 24 593 25 607 0.0 833
Msa0623140 AT3G09550.3 71.502 586 148 3 24 593 25 607 0.0 833
Msa0623140 AT3G09550.2 71.502 586 148 3 24 593 32 614 0.0 832
Msa0623140 AT2G31820.1 43.534 549 271 7 60 593 138 662 8.01e-138 416
Msa0623140 AT1G05640.1 44.404 554 270 7 56 593 96 627 7.62e-136 410
Msa0623140 AT5G60070.1 46.595 558 244 13 61 593 20 548 3.40e-135 405
Msa0623140 AT2G01680.1 43.110 508 273 8 99 593 28 532 2.25e-129 390
Msa0623140 AT1G07710.2 44.565 552 264 5 61 593 15 543 2.44e-129 390
Msa0623140 AT1G07710.1 44.565 552 264 5 61 593 15 543 2.44e-129 390
Msa0623140 AT5G02620.4 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa0623140 AT5G02620.1 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa0623140 AT5G02620.3 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa0623140 AT5G02620.5 41.899 537 248 8 61 556 5 518 1.15e-118 363
Msa0623140 AT5G02620.2 41.899 537 248 8 61 556 5 518 1.15e-118 363
Msa0623140 AT1G10340.1 25.202 496 309 12 111 552 51 538 2.25e-34 138
Msa0623140 AT5G54710.1 27.308 520 284 17 68 552 97 557 1.49e-33 135
Msa0623140 AT5G54710.2 27.308 520 284 17 68 552 142 602 2.33e-33 135
Msa0623140 AT1G10340.2 24.593 492 313 11 111 552 51 534 2.38e-33 135
Msa0623140 AT1G34050.1 26.145 524 294 15 108 554 28 535 6.31e-33 134
Msa0623140 AT2G24600.3 26.892 502 290 18 72 538 70 529 3.55e-32 131
Msa0623140 AT2G24600.4 26.892 502 290 18 72 538 70 529 3.55e-32 131
Msa0623140 AT4G03500.2 26.047 549 280 17 58 522 54 560 2.29e-30 126
Msa0623140 AT4G03500.3 26.047 549 280 17 58 522 54 560 2.29e-30 126
Msa0623140 AT4G03500.1 26.047 549 280 17 58 522 77 583 2.43e-30 126
Msa0623140 AT1G03670.1 25.581 516 328 9 74 562 107 593 2.56e-26 114
Msa0623140 AT4G03490.3 27.674 430 267 12 133 551 180 576 5.97e-25 110
Msa0623140 AT4G03490.5 27.830 424 262 12 133 545 170 560 7.94e-25 109
Msa0623140 AT4G03490.4 27.830 424 262 12 133 545 180 570 8.14e-25 109
Msa0623140 AT4G03460.2 26.406 409 256 8 128 522 158 535 8.35e-24 106
Msa0623140 AT4G03460.1 26.406 409 256 8 128 522 190 567 9.03e-24 106
Msa0623140 AT4G03490.2 24.915 586 332 18 65 574 9 562 1.03e-23 106
Msa0623140 AT5G50140.1 22.913 515 297 16 126 563 22 513 1.05e-23 105
Msa0623140 AT4G03490.1 25.277 542 318 15 65 551 19 528 2.42e-23 105
Msa0623140 AT5G54700.2 28.435 313 159 9 223 523 20 279 1.39e-22 99.8
Msa0623140 AT4G10720.1 24.096 415 258 12 169 559 40 421 3.32e-22 100
Msa0623140 AT5G54700.1 27.809 356 182 12 202 523 2 316 6.75e-22 99.8
Msa0623140 AT4G10720.2 25.076 327 210 6 169 477 40 349 1.96e-21 97.4
Msa0623140 AT4G14390.2 24.722 449 281 11 128 556 226 637 2.16e-20 95.9
Msa0623140 AT4G14390.4 24.722 449 281 11 128 556 245 656 2.63e-20 95.5
Msa0623140 AT4G14390.1 24.889 450 280 12 128 556 245 657 2.98e-20 95.5
Msa0623140 AT4G03480.1 26.076 395 232 9 118 482 295 659 1.57e-19 93.2
Msa0623140 AT4G03480.2 26.076 395 232 9 118 482 292 656 1.65e-19 93.2
Msa0623140 AT4G05040.3 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0623140 AT4G05040.2 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0623140 AT4G05040.5 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0623140 AT4G05040.4 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0623140 AT4G05040.1 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0623140 AT4G05040.6 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0623140 AT4G05040.7 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0623140 AT5G15500.2 23.427 461 288 15 130 559 1 427 2.70e-18 88.2
Msa0623140 AT2G24600.2 26.420 405 232 14 72 450 70 434 1.75e-17 86.3
Msa0623140 AT2G24600.1 26.420 405 232 14 72 450 70 434 1.75e-17 86.3
Msa0623140 AT4G03450.2 24.505 555 306 17 58 528 45 570 1.81e-17 86.7
Msa0623140 AT5G51160.1 25.000 352 198 8 157 478 38 353 2.38e-17 85.5
Msa0623140 AT4G03450.1 24.505 555 306 17 58 528 45 570 5.28e-17 85.1
Msa0623140 AT4G03470.1 26.044 503 294 18 77 542 157 618 1.34e-16 84.0
Msa0623140 AT3G04140.1 27.622 286 160 8 100 342 105 386 2.92e-16 82.8
Msa0623140 AT1G14500.1 22.769 325 215 5 169 475 40 346 3.84e-15 78.6
Msa0623140 AT2G03430.1 34.659 176 106 5 135 306 16 186 8.50e-15 74.7
Msa0623140 AT4G14390.3 26.748 329 195 8 128 443 226 521 4.92e-13 72.4
Msa0623140 AT5G54620.1 29.570 186 119 2 169 342 40 225 8.08e-13 71.2
Msa0623140 AT5G54610.1 27.717 184 123 2 169 342 40 223 8.13e-13 71.2
Msa0623140 AT3G18670.1 23.111 450 296 12 123 530 113 554 1.09e-12 71.2
Msa0623140 AT2G28840.1 27.273 209 129 7 132 322 12 215 2.01e-12 70.1
Msa0623140 AT4G03440.1 23.126 467 308 12 104 550 258 693 3.95e-12 69.7
Msa0623140 AT4G03440.2 23.126 467 308 12 104 550 251 686 4.36e-12 69.7
Msa0623140 AT4G03440.3 23.126 467 308 12 104 550 251 686 4.36e-12 69.7
Msa0623140 AT4G14400.2 23.242 512 310 20 75 538 71 547 4.01e-11 66.2
Msa0623140 AT4G14400.3 23.242 512 310 20 75 538 71 547 4.01e-11 66.2
Msa0623140 AT3G18670.2 23.684 304 183 7 236 530 346 609 4.70e-11 66.2
Msa0623140 AT4G14400.1 23.242 512 310 20 75 538 137 613 5.68e-11 65.9

Find 171 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTGCATATGGCTGTAAA+AGG 0.222840 4_3:-334681 Msa0623140:CDS
TTATCCTTTCCAACGTAATT+TGG 0.223179 4_3:+333036 None:intergenic
TGATATTGATTCTCAGATAA+TGG 0.227356 4_3:-335935 Msa0623140:CDS
AATCATCATAATAATAATTT+AGG 0.249295 4_3:+336404 None:intergenic
GTGGAGTTTCCTCAGCTCTT+TGG 0.250605 4_3:+333611 None:intergenic
GTTATGCTTCCTGACAAATT+TGG 0.259995 4_3:-334609 Msa0623140:CDS
GAAAGGAAAGAGGGAGGAAA+TGG 0.261658 4_3:+336522 None:intergenic
GAACGAGTGGGCTGCAATTC+TGG 0.267500 4_3:-333253 Msa0623140:CDS
TGTTATAGATCATAACAAAT+TGG 0.278217 4_3:-332814 Msa0623140:three_prime_UTR
ACCTCCAATTTCAACCTTCC+AGG 0.283929 4_3:-336317 Msa0623140:intron
ACTCAAAGAGATTCAGAAAA+AGG 0.285730 4_3:-336180 Msa0623140:CDS
AAGGACATCCTCTCTCGATA+TGG 0.290107 4_3:-333762 Msa0623140:CDS
CCAACCTGGAAGGTTGAAAT+TGG 0.303178 4_3:+336313 None:intergenic
TTCATTTGCAGCCATTGTTC+AGG 0.330505 4_3:-334964 Msa0623140:intron
CTGCTTCTCTTTCTTCCTCA+TGG 0.331730 4_3:+333149 None:intergenic
GCAATTCTGGTTACAGTTGT+TGG 0.335512 4_3:-333240 Msa0623140:CDS
GATATTGATTCTCAGATAAT+GGG 0.350929 4_3:-335934 Msa0623140:CDS
AATTCGATCAACCTCGGAAT+TGG 0.351942 4_3:+333080 None:intergenic
AAAGGAAAGAGGGAGGAAAT+GGG 0.356439 4_3:+336523 None:intergenic
TGAAGAGTCATCAGATATAA+AGG 0.366092 4_3:-333781 Msa0623140:CDS
TAATAAGTGTTGGCTTTGAT+TGG 0.372963 4_3:+336463 None:intergenic
TTTGTTTACTGCTGCTGAAA+AGG 0.377535 4_3:-335815 Msa0623140:CDS
AGATGGCGACGGAGATATTT+CGG 0.380641 4_3:+336125 None:intergenic
GTTCAAATTACCCTTGTTAG+AGG 0.385400 4_3:-333390 Msa0623140:CDS
GTGATAATATCCGGAGTTAT+TGG 0.388823 4_3:-333213 Msa0623140:CDS
GTGAAACTAAAGCAGAGAAA+AGG 0.392404 4_3:-333368 Msa0623140:CDS
TTTGCATTTAGCTGCTCGTC+CGG 0.403120 4_3:-334781 Msa0623140:CDS
GTCAAAGGATGGCAGCTTGT+TGG 0.404723 4_3:-334832 Msa0623140:CDS
GGGTCATCTTGATGTTGTTA+AGG 0.408314 4_3:-335794 Msa0623140:CDS
AGAAAGAGAAGCAGCTAGCC+AGG 0.411228 4_3:-333140 Msa0623140:CDS
AGGACATCCTCTCTCGATAT+GGG 0.415929 4_3:-333761 Msa0623140:CDS
CACCACTGTCACCGAGTTTG+TGG 0.435710 4_3:+333569 None:intergenic
CTCGTCGCTGCTGTTATAAG+TGG 0.455896 4_3:+334885 None:intergenic
TCCGTCGTCCGCGACGGCGC+CGG 0.456322 4_3:-336103 Msa0623140:CDS
ATGGGAAAGTCGTGAGAAAG+AGG 0.462631 4_3:+336541 None:intergenic
AGGCTTTCACGATTTCTGTA+TGG 0.466846 4_3:+334757 None:intergenic
TTGCATTTAGCTGCTCGTCC+GGG 0.469773 4_3:-334780 Msa0623140:CDS
GCTGCTATATTCACCGTACC+TGG 0.472210 4_3:-333519 Msa0623140:CDS
TCCTCAGTTCGTCCCTTGGT+TGG 0.473921 4_3:+333715 None:intergenic
GAGTGGAAGGAAAGGAAAGA+GGG 0.474346 4_3:+336513 None:intergenic
CATTTACGTTTGTGTCTGGC+AGG 0.475438 4_3:+333859 None:intergenic
TTGTTTACTGCTGCTGAAAA+GGG 0.479987 4_3:-335814 Msa0623140:CDS
GCTCAGCAAAGACCCTCAAT+TGG 0.481998 4_3:-334733 Msa0623140:CDS
CTTTAGTTTCACCTCTAACA+AGG 0.482809 4_3:+333379 None:intergenic
CCAAGCTTAAGCAAAACTAT+TGG 0.486154 4_3:-334924 Msa0623140:CDS
GAAAAGATGAGTGGAAGGAA+AGG 0.490183 4_3:+336505 None:intergenic
TGAGTTGGATCCACTTCTCC+TGG 0.491866 4_3:+333122 None:intergenic
GGGGATAACGATGATGGATC+AGG 0.492044 4_3:-333495 Msa0623140:CDS
CAGTCCTCCGAGCCAATTGA+GGG 0.492228 4_3:+334721 None:intergenic
TAATAATAATTTAGGAAGGA+AGG 0.493048 4_3:+336412 None:intergenic
TCCTTTCAAACTCAGGAAAA+CGG 0.494021 4_3:-336074 Msa0623140:CDS
TGAGTGGAAGGAAAGGAAAG+AGG 0.495388 4_3:+336512 None:intergenic
TGAAACTAAAGCAGAGAAAA+GGG 0.501105 4_3:-333367 Msa0623140:CDS
AGGGCAAACTGCATTGCATA+TGG 0.501695 4_3:-334691 Msa0623140:CDS
TCAGCTCTTTGGATATATTG+TGG 0.502735 4_3:+333622 None:intergenic
GGTCATGATAGAAGAGTAGA+GGG 0.503098 4_3:+336337 None:intergenic
ATCGTTATCCCCACCAGGTA+CGG 0.503271 4_3:+333506 None:intergenic
TTGAGGGTCTTTGCTGAGCA+AGG 0.503619 4_3:+334737 None:intergenic
AATGCATTTACGTTTGTGTC+TGG 0.503703 4_3:+333855 None:intergenic
TTGGAAATTGCAAGATCCAA+TGG 0.508338 4_3:-334813 Msa0623140:CDS
AAGTACGTGAAGCAAGTAAC+GGG 0.508570 4_3:-336030 Msa0623140:CDS
GCTCGGAGGACTGACAAGAA+AGG 0.509842 4_3:-334711 Msa0623140:CDS
AAAGAGAAAAGATGAGTGGA+AGG 0.510130 4_3:+336500 None:intergenic
GACGGAGATATTTCGGAAAG+TGG 0.514348 4_3:+336132 None:intergenic
CTCGGAGGACTGACAAGAAA+GGG 0.519611 4_3:-334710 Msa0623140:CDS
TTCACGATTTCTGTATGGCC+CGG 0.525638 4_3:+334762 None:intergenic
TATGCATTTGTATAAATCAC+AGG 0.525663 4_3:-332732 Msa0623140:three_prime_UTR
GTTGGAGGAGTGATAATATC+CGG 0.527167 4_3:-333222 Msa0623140:CDS
GCCGGCGCCGTCGCGGACGA+CGG 0.529146 4_3:+336102 None:intergenic
CATTGCATATGGCTGTAAAA+GGG 0.531587 4_3:-334680 Msa0623140:CDS
AAGACCAAATTACGTTGGAA+AGG 0.532286 4_3:-333040 Msa0623140:three_prime_UTR
TGCTACAAGAAATGTCTTGG+AGG 0.533293 4_3:-332598 Msa0623140:three_prime_UTR
GAAAGATGTTCACACACAGC+TGG 0.535788 4_3:-333673 Msa0623140:CDS
CCTGGTGGGGATAACGATGA+TGG 0.535860 4_3:-333501 Msa0623140:CDS
GCCATCTCCGTCGTCCGCGA+CGG 0.536247 4_3:-336109 Msa0623140:CDS
GCTGTGTTGCCAAATTTGTC+AGG 0.537540 4_3:+334600 None:intergenic
GGAAAACGGATCGATCAAGC+AGG 0.537963 4_3:-336060 Msa0623140:CDS
CTATATTCACCGTACCTGGT+GGG 0.538227 4_3:-333515 Msa0623140:CDS
CAGCATTGCATGTGGCAACC+AGG 0.541967 4_3:-334581 Msa0623140:CDS
AAGCAGCTAGCCAGGAGAAG+TGG 0.542236 4_3:-333132 Msa0623140:CDS
TCAGTCCTCCGAGCCAATTG+AGG 0.545108 4_3:+334720 None:intergenic
TCGGAAAGTGGGTTCTGTGT+TGG 0.547229 4_3:+336144 None:intergenic
GACTAGAGCCGGCGCCGTCG+CGG 0.548417 4_3:+336095 None:intergenic
GAAAGTCGTGAGAAAGAGGG+AGG 0.548432 4_3:+336545 None:intergenic
TTGCATATTGCTGCTAGTCA+AGG 0.549825 4_3:-335709 Msa0623140:CDS
CAGATAATGGGTACTTTGAG+TGG 0.549949 4_3:-335922 Msa0623140:CDS
TGGAAGGAAAGGAAAGAGGG+AGG 0.550810 4_3:+336516 None:intergenic
AAGTCGTGAGAAAGAGGGAG+GGG 0.550978 4_3:+336547 None:intergenic
TGGGAAAGTCGTGAGAAAGA+GGG 0.551251 4_3:+336542 None:intergenic
CTCTGAATGATGCCATGAGT+TGG 0.551504 4_3:+333107 None:intergenic
AAGAGCTGAGGAAACTCCAC+AGG 0.552962 4_3:-333608 Msa0623140:CDS
ATCTATGTAAACTCTTGAAG+TGG 0.553547 4_3:+332894 None:intergenic
ATCTATGTAAACTCTTGAAG+TGG 0.553547 4_3:+332979 None:intergenic
ATTCTGGTTACAGTTGTTGG+AGG 0.554005 4_3:-333237 Msa0623140:CDS
GATTAACAAGCTCATGTGGT+TGG 0.554304 4_3:-333334 Msa0623140:CDS
AAGGGACGAACTGAGGAAAA+CGG 0.554349 4_3:-333709 Msa0623140:CDS
AGGTCATGATAGAAGAGTAG+AGG 0.556929 4_3:+336336 None:intergenic
AAAGTCGTGAGAAAGAGGGA+GGG 0.557510 4_3:+336546 None:intergenic
ACCTGGAAGGTTGAAATTGG+AGG 0.558297 4_3:+336316 None:intergenic
GCCGTCGCGGACGACGGAGA+TGG 0.559929 4_3:+336108 None:intergenic
CCATCATCGTTATCCCCACC+AGG 0.564948 4_3:+333501 None:intergenic
TTTAGTTTCACCTCTAACAA+GGG 0.565107 4_3:+333380 None:intergenic
GTTTGAAGACCAAATTACGT+TGG 0.566835 4_3:-333045 Msa0623140:three_prime_UTR
GCTACAAGAAATGTCTTGGA+GGG 0.566909 4_3:-332597 Msa0623140:three_prime_UTR
GAGTTTGAAAGGACTAGAGC+CGG 0.567456 4_3:+336084 None:intergenic
GCAAAGACCCTCAATTGGCT+CGG 0.569896 4_3:-334728 Msa0623140:CDS
GCTCTAGTCCTTTCAAACTC+AGG 0.572097 4_3:-336081 Msa0623140:CDS
CATTGTTAATTCCTTCCCTG+TGG 0.572226 4_3:+333592 None:intergenic
AGCAGAGAAAAGGGTAGTTG+AGG 0.573019 4_3:-333358 Msa0623140:CDS
TCGTCGGTCGGAGGAACGAG+TGG 0.574175 4_3:-333266 Msa0623140:CDS
CGCTGCTGTTATAAGTGGAG+TGG 0.575162 4_3:+334890 None:intergenic
GCTGCTAGTCAAGGACATCA+TGG 0.575482 4_3:-335700 Msa0623140:intron
ATAAGTGGAGTGGCGTTGGA+TGG 0.578501 4_3:+334900 None:intergenic
GGAAGGAAGGATGAAAGTGA+AGG 0.582860 4_3:+336425 None:intergenic
GCAGCTCAAAGAGGTGATGT+TGG 0.583391 4_3:-335979 Msa0623140:CDS
GTGAATGAAGAGAATGAACT+TGG 0.587406 4_3:-335847 Msa0623140:CDS
TCTGAGAATCAATATCAAGA+AGG 0.587872 4_3:+335941 None:intergenic
ACGGAGATATTTCGGAAAGT+GGG 0.587917 4_3:+336133 None:intergenic
TGTTATAAGTGGAGTGGCGT+TGG 0.592828 4_3:+334896 None:intergenic
TTCAGAGTTCTCCAATTCCG+AGG 0.592884 4_3:-333091 Msa0623140:CDS
GCATGAGTCATAATAAGTGT+TGG 0.594195 4_3:+336453 None:intergenic
ATCATAATAATAATTTAGGA+AGG 0.594731 4_3:+336408 None:intergenic
GCTATATTCACCGTACCTGG+TGG 0.598478 4_3:-333516 Msa0623140:CDS
ATCTAGTAAAACCTGAACAA+TGG 0.600510 4_3:+334953 None:intergenic
GCCTCTTCGTACATAGTCGT+CGG 0.600824 4_3:-333282 Msa0623140:CDS
CGCATAAATTCGATCAACCT+CGG 0.601587 4_3:+333074 None:intergenic
TGATAAAGAGAAAAGATGAG+TGG 0.602428 4_3:+336496 None:intergenic
CGTACATAGTCGTCGGTCGG+AGG 0.602499 4_3:-333275 Msa0623140:CDS
GTTGGCTTCTGTCTGTACCT+CGG 0.602568 4_3:-333316 Msa0623140:CDS
CTGAGGAAACTCCACAGGGA+AGG 0.603249 4_3:-333603 Msa0623140:CDS
TATATTCACCGTACCTGGTG+GGG 0.603908 4_3:-333514 Msa0623140:CDS
GAAGTACGTGAAGCAAGTAA+CGG 0.604371 4_3:-336031 Msa0623140:CDS
GGAGAAGTGGATCCAACTCA+TGG 0.605497 4_3:-333119 Msa0623140:CDS
AGAAGAAGAACCAACCTGGA+AGG 0.609441 4_3:+336303 None:intergenic
GACGAGAGGGCACGTCGAAG+TGG 0.609993 4_3:-334868 Msa0623140:CDS
GGTGAATGAACTTCTGTCAA+AGG 0.612992 4_3:-334847 Msa0623140:CDS
TTATAACAGCAGCGACGAGA+GGG 0.613377 4_3:-334881 Msa0623140:CDS
AATGAACTTCTGTCAAAGGA+TGG 0.613527 4_3:-334843 Msa0623140:CDS
TCTGATGACTCTTCAGAGAG+AGG 0.615975 4_3:+333789 None:intergenic
AATTAACAATGCCACAAACT+CGG 0.616650 4_3:-333580 Msa0623140:CDS
CGTCGGTCGGAGGAACGAGT+GGG 0.619749 4_3:-333265 Msa0623140:CDS
ACCAACCAAGGGACGAACTG+AGG 0.620407 4_3:-333716 Msa0623140:CDS
CTAACGAGCTCAACCAACCA+AGG 0.621035 4_3:-333728 Msa0623140:CDS
CTTCATCTAGCAGCTCAAAG+AGG 0.623399 4_3:-335988 Msa0623140:CDS
CTTCGTACATAGTCGTCGGT+CGG 0.624785 4_3:-333278 Msa0623140:CDS
CTTATAACAGCAGCGACGAG+AGG 0.631608 4_3:-334882 Msa0623140:CDS
TGCCACAAACTCGGTGACAG+TGG 0.632510 4_3:-333571 Msa0623140:CDS
AGTCATAGTGCCAATAACTC+CGG 0.636725 4_3:+333203 None:intergenic
TGGCAACACAGCATTGCATG+TGG 0.637663 4_3:-334589 Msa0623140:CDS
GGACATCCTCTCTCGATATG+GGG 0.638757 4_3:-333760 Msa0623140:CDS
TGACTAGCAGCAATATGCAA+AGG 0.639675 4_3:+335712 None:intergenic
ACAATATATCCAAAGAGCTG+AGG 0.644642 4_3:-333620 Msa0623140:CDS
AAAATTCTCAGTAACAGACT+AGG 0.646190 4_3:+332620 None:intergenic
AAGACCCTCAATTGGCTCGG+AGG 0.651684 4_3:-334725 Msa0623140:CDS
GCGGACGACGGAGATGGCGA+CGG 0.652416 4_3:+336114 None:intergenic
GGCTTCTGTCTGTACCTCGG+TGG 0.655940 4_3:-333313 Msa0623140:CDS
GAAAACGGATCGATCAAGCA+GGG 0.663000 4_3:-336059 Msa0623140:CDS
TAACGATGATGGATCAGGAG+TGG 0.663853 4_3:-333490 Msa0623140:CDS
TAAGTGCCCCATATCGAGAG+AGG 0.665007 4_3:+333754 None:intergenic
AGGTGATTAACAAGCTCATG+TGG 0.671742 4_3:-333338 Msa0623140:CDS
ACCGACGACTATGTACGAAG+AGG 0.681066 4_3:+333281 None:intergenic
CACAAACTCGGTGACAGTGG+TGG 0.691648 4_3:-333568 Msa0623140:CDS
TAACGAGCTCAACCAACCAA+GGG 0.693605 4_3:-333727 Msa0623140:CDS
ACTAGGATAACATATAGACC+AGG 0.706188 4_3:+332637 None:intergenic
CTATGACTTACTATGTAGTG+AGG 0.708286 4_3:-333188 Msa0623140:CDS
CTAAGAGAACAAGATCCATG+AGG 0.720939 4_3:-333164 Msa0623140:CDS
AGAGCTGAGGAAACTCCACA+GGG 0.730372 4_3:-333607 Msa0623140:CDS
AAAAGGGCAGAGCTGTGACG+TGG 0.742175 4_3:-334664 Msa0623140:CDS
GTCATGATAGAAGAGTAGAG+GGG 0.743790 4_3:+336338 None:intergenic
AGAGGCAATAAATGCCACCG+AGG 0.784742 4_3:+333299 None:intergenic
GATTTGTGAGAAGAATAACA+CGG 0.801731 4_3:-332705 Msa0623140:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATCATAATAATAATTT+AGG + chr4_3:332747-332766 None:intergenic 10.0%
!! ATCATAATAATAATTTAGGA+AGG + chr4_3:332743-332762 None:intergenic 15.0%
!!! TATTTTGCTTTTTGTAAAAA+TGG - chr4_3:333560-333579 Msa0623140:CDS 15.0%
!!! ATTTTGCTTTTTGTAAAAAT+GGG - chr4_3:333561-333580 Msa0623140:CDS 15.0%
!!! TTTTTTTAAGAAGCTATTAA+TGG - chr4_3:333953-333972 Msa0623140:intron 15.0%
!! AAAAATTAAAAGAAAACAAG+AGG + chr4_3:335152-335171 None:intergenic 15.0%
!!! AATATTAGATTTTTCATTCA+AGG + chr4_3:335225-335244 None:intergenic 15.0%
!! TAATAATAATTTAGGAAGGA+AGG + chr4_3:332739-332758 None:intergenic 20.0%
!!! TATTTTGAATGAATTGACTT+TGG - chr4_3:333595-333614 Msa0623140:CDS 20.0%
!!! TTTTTTTTGGAACATATTCT+TGG - chr4_3:333793-333812 Msa0623140:CDS 20.0%
!! AATTTAATCAGATATACTTC+TGG + chr4_3:333841-333860 None:intergenic 20.0%
!! TCATAATTGAGTTTCTTTAT+TGG + chr4_3:333918-333937 None:intergenic 20.0%
!! TAATACTACTACTACAAATA+TGG + chr4_3:334726-334745 None:intergenic 20.0%
!! GCAACTACTTAAATAAATAA+AGG + chr4_3:334954-334973 None:intergenic 20.0%
!!! TGTTATTTTGCTTTGAAAAT+TGG - chr4_3:335028-335047 Msa0623140:intron 20.0%
!! TGTTATAGATCATAACAAAT+TGG - chr4_3:336334-336353 Msa0623140:exon 20.0%
! TTACAGTGAAGTAAAAAGAT+GGG + chr4_3:332880-332899 None:intergenic 25.0%
! TTTACAGTGAAGTAAAAAGA+TGG + chr4_3:332881-332900 None:intergenic 25.0%
!!! TGATATTGATTCTCAGATAA+TGG - chr4_3:333213-333232 Msa0623140:CDS 25.0%
!!! GATATTGATTCTCAGATAAT+GGG - chr4_3:333214-333233 Msa0623140:CDS 25.0%
! AACATCAAAACAAAGGAAAA+AGG + chr4_3:333521-333540 None:intergenic 25.0%
!!! TTTTTCCTTTGTTTTGATGT+TGG - chr4_3:333520-333539 Msa0623140:CDS 25.0%
!!! TTTTCCTTTGTTTTGATGTT+GGG - chr4_3:333521-333540 Msa0623140:CDS 25.0%
! AAGGTAAAACGATTTATGTT+TGG - chr4_3:333632-333651 Msa0623140:CDS 25.0%
! AGGTAAAACGATTTATGTTT+GGG - chr4_3:333633-333652 Msa0623140:CDS 25.0%
! TTGAGTATTTATCTTGCTTA+AGG - chr4_3:333889-333908 Msa0623140:intron 25.0%
! CTCAATTATGATGCTATTAA+TGG - chr4_3:333927-333946 Msa0623140:intron 25.0%
! ATTAACTTACTCGAACAAAT+CGG - chr4_3:334676-334695 Msa0623140:CDS 25.0%
! TTTAAGTAGTTGCAAGTATA+AGG - chr4_3:334961-334980 Msa0623140:intron 25.0%
! AAACATGTTATATGAGACAA+GGG - chr4_3:335094-335113 Msa0623140:intron 25.0%
!! TCAATTTTGTCTAGAAGTTT+TGG - chr4_3:335125-335144 Msa0623140:intron 25.0%
!! CAATTTTGTCTAGAAGTTTT+GGG - chr4_3:335126-335145 Msa0623140:intron 25.0%
!!! ATTGTTGTTTCTCAATTTCT+TGG - chr4_3:335242-335261 Msa0623140:intron 25.0%
!! TATATTGTGGACATTTTTGT+TGG + chr4_3:335516-335535 None:intergenic 25.0%
! TATGCATTTGTATAAATCAC+AGG - chr4_3:336416-336435 Msa0623140:five_prime_UTR 25.0%
! TTGTAACACAAAAATATTCC+TGG - chr4_3:336493-336512 Msa0623140:five_prime_UTR 25.0%
TGATAAAGAGAAAAGATGAG+TGG + chr4_3:332655-332674 None:intergenic 30.0%
!! TAATAAGTGTTGGCTTTGAT+TGG + chr4_3:332688-332707 None:intergenic 30.0%
TACAGTGAAGTAAAAAGATG+GGG + chr4_3:332879-332898 None:intergenic 30.0%
ACTCAAAGAGATTCAGAAAA+AGG - chr4_3:332968-332987 Msa0623140:three_prime_UTR 30.0%
TCTGAGAATCAATATCAAGA+AGG + chr4_3:333210-333229 None:intergenic 30.0%
! GTTTCTAAAAAGAATCGATC+TGG - chr4_3:333406-333425 Msa0623140:CDS 30.0%
AAATCCCAACATCAAAACAA+AGG + chr4_3:333528-333547 None:intergenic 30.0%
!!! TTGTTTTGATGTTGGGATTT+TGG - chr4_3:333528-333547 Msa0623140:CDS 30.0%
!! TTTGGCTAAAATTGAGTTGA+AGG - chr4_3:333613-333632 Msa0623140:CDS 30.0%
ACATAGCATTAACTCTACTA+CGG + chr4_3:334024-334043 None:intergenic 30.0%
AATAAGACAACGTTGATGTT+TGG + chr4_3:334058-334077 None:intergenic 30.0%
! AACATCAACGTTGTCTTATT+TGG - chr4_3:334058-334077 Msa0623140:intron 30.0%
AGATTACTACAAAGAACAGA+AGG + chr4_3:334154-334173 None:intergenic 30.0%
ATCTAGTAAAACCTGAACAA+TGG + chr4_3:334198-334217 None:intergenic 30.0%
!! CAATAGTTTTGCTTAAGCTT+GGG + chr4_3:334226-334245 None:intergenic 30.0%
!!! ATGCAAAGCATTTTTTCCAT+TGG + chr4_3:334354-334373 None:intergenic 30.0%
TCATCACTATATGTACTGTA+TGG + chr4_3:334646-334665 None:intergenic 30.0%
TTAACTTACTCGAACAAATC+GGG - chr4_3:334677-334696 Msa0623140:CDS 30.0%
AAGTCTCCAATATTGAAAAC+AGG - chr4_3:334763-334782 Msa0623140:CDS 30.0%
! TAGCTACCTGTTTTCAATAT+TGG + chr4_3:334772-334791 None:intergenic 30.0%
GTGCACACTACTTAAAAAAA+TGG + chr4_3:335064-335083 None:intergenic 30.0%
GAAACATGTTATATGAGACA+AGG - chr4_3:335093-335112 Msa0623140:intron 30.0%
! AAGATTTTCAGCAATGTCAA+GGG + chr4_3:335339-335358 None:intergenic 30.0%
TGAAGAGTCATCAGATATAA+AGG - chr4_3:335367-335386 Msa0623140:intron 30.0%
AATTAACAATGCCACAAACT+CGG - chr4_3:335568-335587 Msa0623140:intron 30.0%
!! TATTGCGCTTTTTACATCTT+TGG - chr4_3:335724-335743 Msa0623140:CDS 30.0%
! TTTAGTTTCACCTCTAACAA+GGG + chr4_3:335771-335790 None:intergenic 30.0%
TGAAACTAAAGCAGAGAAAA+GGG - chr4_3:335781-335800 Msa0623140:CDS 30.0%
TTATCCTTTCCAACGTAATT+TGG + chr4_3:336115-336134 None:intergenic 30.0%
ATCTATGTAAACTCTTGAAG+TGG + chr4_3:336172-336191 None:intergenic 30.0%
ATCTATGTAAACTCTTGAAG+TGG + chr4_3:336172-336191 None:intergenic 30.0%
GATTTGTGAGAAGAATAACA+CGG - chr4_3:336443-336462 Msa0623140:five_prime_UTR 30.0%
AAAATTCTCAGTAACAGACT+AGG + chr4_3:336531-336550 None:intergenic 30.0%
! TTTTGCTACAAGAAATGTCT+TGG - chr4_3:336547-336566 Msa0623140:five_prime_UTR 30.0%
AAAGAGAAAAGATGAGTGGA+AGG + chr4_3:332651-332670 None:intergenic 35.0%
GCATGAGTCATAATAAGTGT+TGG + chr4_3:332698-332717 None:intergenic 35.0%
ACAGTGAAGTAAAAAGATGG+GGG + chr4_3:332878-332897 None:intergenic 35.0%
! TACGCTTTTACGTTTATTGC+AGG - chr4_3:332935-332954 Msa0623140:three_prime_UTR 35.0%
TCCTTTCAAACTCAGGAAAA+CGG - chr4_3:333074-333093 Msa0623140:CDS 35.0%
GTGAATGAAGAGAATGAACT+TGG - chr4_3:333301-333320 Msa0623140:CDS 35.0%
TTTGTTTACTGCTGCTGAAA+AGG - chr4_3:333333-333352 Msa0623140:CDS 35.0%
TTGTTTACTGCTGCTGAAAA+GGG - chr4_3:333334-333353 Msa0623140:CDS 35.0%
GCTTCAAGTAGAATCAGTTA+TGG - chr4_3:333731-333750 Msa0623140:CDS 35.0%
TCAGTTATGGAGACAAAATC+AGG - chr4_3:333744-333763 Msa0623140:CDS 35.0%
CTTGAGTAGCTTCAATTTGT+GGG - chr4_3:334097-334116 Msa0623140:intron 35.0%
! TAGCTTCAATTTGTGGGTTA+TGG - chr4_3:334103-334122 Msa0623140:intron 35.0%
!! CCAATAGTTTTGCTTAAGCT+TGG + chr4_3:334227-334246 None:intergenic 35.0%
CCAAGCTTAAGCAAAACTAT+TGG - chr4_3:334224-334243 Msa0623140:intron 35.0%
AATGAACTTCTGTCAAAGGA+TGG - chr4_3:334305-334324 Msa0623140:intron 35.0%
TTGGAAATTGCAAGATCCAA+TGG - chr4_3:334335-334354 Msa0623140:intron 35.0%
CATTGCATATGGCTGTAAAA+GGG - chr4_3:334468-334487 Msa0623140:intron 35.0%
GTTATGCTTCCTGACAAATT+TGG - chr4_3:334539-334558 Msa0623140:intron 35.0%
TGCTACAAAAAATGCAAACG+AGG - chr4_3:334796-334815 Msa0623140:CDS 35.0%
AAGTAGTTGCAAGTATAAGG+TGG - chr4_3:334964-334983 Msa0623140:intron 35.0%
ACATGCTCAAAAGTAACTAG+GGG + chr4_3:335195-335214 None:intergenic 35.0%
GACATGCTCAAAAGTAACTA+GGG + chr4_3:335196-335215 None:intergenic 35.0%
TGACATGCTCAAAAGTAACT+AGG + chr4_3:335197-335216 None:intergenic 35.0%
AATGCATTTACGTTTGTGTC+TGG + chr4_3:335296-335315 None:intergenic 35.0%
! GAAGATTTTCAGCAATGTCA+AGG + chr4_3:335340-335359 None:intergenic 35.0%
!!! GACATTTTTGTTGGTTCTCT+TGG + chr4_3:335507-335526 None:intergenic 35.0%
TCAGCTCTTTGGATATATTG+TGG + chr4_3:335529-335548 None:intergenic 35.0%
ACAATATATCCAAAGAGCTG+AGG - chr4_3:335528-335547 Msa0623140:intron 35.0%
! TTTTACATCTTTGGCTGTTG+TGG - chr4_3:335733-335752 Msa0623140:CDS 35.0%
GTTCAAATTACCCTTGTTAG+AGG - chr4_3:335758-335777 Msa0623140:CDS 35.0%
! CTTTAGTTTCACCTCTAACA+AGG + chr4_3:335772-335791 None:intergenic 35.0%
GTGAAACTAAAGCAGAGAAA+AGG - chr4_3:335780-335799 Msa0623140:CDS 35.0%
GTGATAATATCCGGAGTTAT+TGG - chr4_3:335935-335954 Msa0623140:CDS 35.0%
CTATGACTTACTATGTAGTG+AGG - chr4_3:335960-335979 Msa0623140:CDS 35.0%
GTTTGAAGACCAAATTACGT+TGG - chr4_3:336103-336122 Msa0623140:CDS 35.0%
AAGACCAAATTACGTTGGAA+AGG - chr4_3:336108-336127 Msa0623140:CDS 35.0%
ACTAGGATAACATATAGACC+AGG + chr4_3:336514-336533 None:intergenic 35.0%
AAAGGAAAGAGGGAGGAAAT+GGG + chr4_3:332628-332647 None:intergenic 40.0%
GAAAAGATGAGTGGAAGGAA+AGG + chr4_3:332646-332665 None:intergenic 40.0%
GTCATGATAGAAGAGTAGAG+GGG + chr4_3:332813-332832 None:intergenic 40.0%
GGTCATGATAGAAGAGTAGA+GGG + chr4_3:332814-332833 None:intergenic 40.0%
AGGTCATGATAGAAGAGTAG+AGG + chr4_3:332815-332834 None:intergenic 40.0%
AGATAGAAGAAGAACCAACC+TGG + chr4_3:332852-332871 None:intergenic 40.0%
! GCTTTTACGTTTATTGCAGG+TGG - chr4_3:332938-332957 Msa0623140:three_prime_UTR 40.0%
ACGGAGATATTTCGGAAAGT+GGG + chr4_3:333018-333037 None:intergenic 40.0%
! TCCGTTTTCCTGAGTTTGAA+AGG + chr4_3:333078-333097 None:intergenic 40.0%
GAAGTACGTGAAGCAAGTAA+CGG - chr4_3:333117-333136 Msa0623140:CDS 40.0%
AAGTACGTGAAGCAAGTAAC+GGG - chr4_3:333118-333137 Msa0623140:CDS 40.0%
!! CAGATAATGGGTACTTTGAG+TGG - chr4_3:333226-333245 Msa0623140:CDS 40.0%
GGGTCATCTTGATGTTGTTA+AGG - chr4_3:333354-333373 Msa0623140:CDS 40.0%
TGACTAGCAGCAATATGCAA+AGG + chr4_3:333439-333458 None:intergenic 40.0%
TTGCATATTGCTGCTAGTCA+AGG - chr4_3:333439-333458 Msa0623140:CDS 40.0%
GCTACTCAAGGTCTAACAAT+TGG + chr4_3:334087-334106 None:intergenic 40.0%
CCACAAATTGAAGCTACTCA+AGG + chr4_3:334099-334118 None:intergenic 40.0%
CCTTGAGTAGCTTCAATTTG+TGG - chr4_3:334096-334115 Msa0623140:intron 40.0%
TTCATTTGCAGCCATTGTTC+AGG - chr4_3:334184-334203 Msa0623140:intron 40.0%
GGTGAATGAACTTCTGTCAA+AGG - chr4_3:334301-334320 Msa0623140:intron 40.0%
AGGCTTTCACGATTTCTGTA+TGG + chr4_3:334394-334413 None:intergenic 40.0%
GCATTGCATATGGCTGTAAA+AGG - chr4_3:334467-334486 Msa0623140:intron 40.0%
CATTGTTAATTCCTTCCCTG+TGG + chr4_3:335559-335578 None:intergenic 40.0%
AGGTGATTAACAAGCTCATG+TGG - chr4_3:335810-335829 Msa0623140:CDS 40.0%
GATTAACAAGCTCATGTGGT+TGG - chr4_3:335814-335833 Msa0623140:CDS 40.0%
! GCAATTCTGGTTACAGTTGT+TGG - chr4_3:335908-335927 Msa0623140:CDS 40.0%
! ATTCTGGTTACAGTTGTTGG+AGG - chr4_3:335911-335930 Msa0623140:CDS 40.0%
GTTGGAGGAGTGATAATATC+CGG - chr4_3:335926-335945 Msa0623140:CDS 40.0%
AGTCATAGTGCCAATAACTC+CGG + chr4_3:335948-335967 None:intergenic 40.0%
CTAAGAGAACAAGATCCATG+AGG - chr4_3:335984-336003 Msa0623140:CDS 40.0%
AATTCGATCAACCTCGGAAT+TGG + chr4_3:336071-336090 None:intergenic 40.0%
CGCATAAATTCGATCAACCT+CGG + chr4_3:336077-336096 None:intergenic 40.0%
TGCTACAAGAAATGTCTTGG+AGG - chr4_3:336550-336569 Msa0623140:five_prime_UTR 40.0%
GCTACAAGAAATGTCTTGGA+GGG - chr4_3:336551-336570 Msa0623140:five_prime_UTR 40.0%
AAAGTCGTGAGAAAGAGGGA+GGG + chr4_3:332605-332624 None:intergenic 45.0%
TGGGAAAGTCGTGAGAAAGA+GGG + chr4_3:332609-332628 None:intergenic 45.0%
ATGGGAAAGTCGTGAGAAAG+AGG + chr4_3:332610-332629 None:intergenic 45.0%
GAAAGGAAAGAGGGAGGAAA+TGG + chr4_3:332629-332648 None:intergenic 45.0%
GAGTGGAAGGAAAGGAAAGA+GGG + chr4_3:332638-332657 None:intergenic 45.0%
TGAGTGGAAGGAAAGGAAAG+AGG + chr4_3:332639-332658 None:intergenic 45.0%
GGAAGGAAGGATGAAAGTGA+AGG + chr4_3:332726-332745 None:intergenic 45.0%
ACCTCCAATTTCAACCTTCC+AGG - chr4_3:332831-332850 Msa0623140:three_prime_UTR 45.0%
ACCTGGAAGGTTGAAATTGG+AGG + chr4_3:332835-332854 None:intergenic 45.0%
CCAACCTGGAAGGTTGAAAT+TGG + chr4_3:332838-332857 None:intergenic 45.0%
CCAATTTCAACCTTCCAGGT+TGG - chr4_3:332835-332854 Msa0623140:three_prime_UTR 45.0%
AGAAGAAGAACCAACCTGGA+AGG + chr4_3:332848-332867 None:intergenic 45.0%
GACGGAGATATTTCGGAAAG+TGG + chr4_3:333019-333038 None:intergenic 45.0%
AGATGGCGACGGAGATATTT+CGG + chr4_3:333026-333045 None:intergenic 45.0%
GAGTTTGAAAGGACTAGAGC+CGG + chr4_3:333067-333086 None:intergenic 45.0%
GCTCTAGTCCTTTCAAACTC+AGG - chr4_3:333067-333086 Msa0623140:exon 45.0%
GAAAACGGATCGATCAAGCA+GGG - chr4_3:333089-333108 Msa0623140:CDS 45.0%
CTTCATCTAGCAGCTCAAAG+AGG - chr4_3:333160-333179 Msa0623140:CDS 45.0%
TGTTATAAGTGGAGTGGCGT+TGG + chr4_3:334255-334274 None:intergenic 45.0%
TTATAACAGCAGCGACGAGA+GGG - chr4_3:334267-334286 Msa0623140:intron 45.0%
TTTGCATTTAGCTGCTCGTC+CGG - chr4_3:334367-334386 Msa0623140:intron 45.0%
TTCACGATTTCTGTATGGCC+CGG + chr4_3:334389-334408 None:intergenic 45.0%
AGGGCAAACTGCATTGCATA+TGG - chr4_3:334457-334476 Msa0623140:intron 45.0%
GCTGTGTTGCCAAATTTGTC+AGG + chr4_3:334551-334570 None:intergenic 45.0%
AACCAGGAAAAAGCGAGTAG+AGG - chr4_3:334583-334602 Msa0623140:CDS 45.0%
!!! TACCTCTACTCGCTTTTTCC+TGG + chr4_3:334588-334607 None:intergenic 45.0%
CATTTACGTTTGTGTCTGGC+AGG + chr4_3:335292-335311 None:intergenic 45.0%
!! CAATGTCAAGGGCTGTTTTG+TGG + chr4_3:335328-335347 None:intergenic 45.0%
TCTGATGACTCTTCAGAGAG+AGG + chr4_3:335362-335381 None:intergenic 45.0%
AAGGACATCCTCTCTCGATA+TGG - chr4_3:335386-335405 Msa0623140:intron 45.0%
AGGACATCCTCTCTCGATAT+GGG - chr4_3:335387-335406 Msa0623140:intron 45.0%
TAACGAGCTCAACCAACCAA+GGG - chr4_3:335421-335440 Msa0623140:intron 45.0%
AAGGGACGAACTGAGGAAAA+CGG - chr4_3:335439-335458 Msa0623140:intron 45.0%
GAAAGATGTTCACACACAGC+TGG - chr4_3:335475-335494 Msa0623140:intron 45.0%
CTATATTCACCGTACCTGGT+GGG - chr4_3:335633-335652 Msa0623140:intron 45.0%
TATATTCACCGTACCTGGTG+GGG - chr4_3:335634-335653 Msa0623140:intron 45.0%
!! TAACGATGATGGATCAGGAG+TGG - chr4_3:335658-335677 Msa0623140:intron 45.0%
AGCAGAGAAAAGGGTAGTTG+AGG - chr4_3:335790-335809 Msa0623140:CDS 45.0%
CTGCTTCTCTTTCTTCCTCA+TGG + chr4_3:336002-336021 None:intergenic 45.0%
CTCTGAATGATGCCATGAGT+TGG + chr4_3:336044-336063 None:intergenic 45.0%
TTCAGAGTTCTCCAATTCCG+AGG - chr4_3:336057-336076 Msa0623140:CDS 45.0%
!! GAGTTTTGTGCACTTCAGCA+TGG - chr4_3:336387-336406 Msa0623140:five_prime_UTR 45.0%
!!! TTTTGTATTTTTTTTTTTTT+TGG - chr4_3:333780-333799 Msa0623140:CDS 5.0%
AAGTCGTGAGAAAGAGGGAG+GGG + chr4_3:332604-332623 None:intergenic 50.0%
GAAAGTCGTGAGAAAGAGGG+AGG + chr4_3:332606-332625 None:intergenic 50.0%
TGGAAGGAAAGGAAAGAGGG+AGG + chr4_3:332635-332654 None:intergenic 50.0%
! TCGGAAAGTGGGTTCTGTGT+TGG + chr4_3:333007-333026 None:intergenic 50.0%
GGAAAACGGATCGATCAAGC+AGG - chr4_3:333088-333107 Msa0623140:CDS 50.0%
GCAGCTCAAAGAGGTGATGT+TGG - chr4_3:333169-333188 Msa0623140:CDS 50.0%
GCTGCTAGTCAAGGACATCA+TGG - chr4_3:333448-333467 Msa0623140:CDS 50.0%
! ATAAGTGGAGTGGCGTTGGA+TGG + chr4_3:334251-334270 None:intergenic 50.0%
CGCTGCTGTTATAAGTGGAG+TGG + chr4_3:334261-334280 None:intergenic 50.0%
CTCGTCGCTGCTGTTATAAG+TGG + chr4_3:334266-334285 None:intergenic 50.0%
CTTATAACAGCAGCGACGAG+AGG - chr4_3:334266-334285 Msa0623140:intron 50.0%
GTCAAAGGATGGCAGCTTGT+TGG - chr4_3:334316-334335 Msa0623140:intron 50.0%
TTGCATTTAGCTGCTCGTCC+GGG - chr4_3:334368-334387 Msa0623140:intron 50.0%
TTGAGGGTCTTTGCTGAGCA+AGG + chr4_3:334414-334433 None:intergenic 50.0%
GCTCAGCAAAGACCCTCAAT+TGG - chr4_3:334415-334434 Msa0623140:intron 50.0%
GCAAAGACCCTCAATTGGCT+CGG - chr4_3:334420-334439 Msa0623140:intron 50.0%
CTCGGAGGACTGACAAGAAA+GGG - chr4_3:334438-334457 Msa0623140:intron 50.0%
TGGCAACACAGCATTGCATG+TGG - chr4_3:334559-334578 Msa0623140:intron 50.0%
GGACATCCTCTCTCGATATG+GGG - chr4_3:335388-335407 Msa0623140:intron 50.0%
TAAGTGCCCCATATCGAGAG+AGG + chr4_3:335397-335416 None:intergenic 50.0%
CTAACGAGCTCAACCAACCA+AGG - chr4_3:335420-335439 Msa0623140:intron 50.0%
! GTTTTCCTCAGTTCGTCCCT+TGG + chr4_3:335440-335459 None:intergenic 50.0%
GTGGAGTTTCCTCAGCTCTT+TGG + chr4_3:335540-335559 None:intergenic 50.0%
AAGAGCTGAGGAAACTCCAC+AGG - chr4_3:335540-335559 Msa0623140:intron 50.0%
AGAGCTGAGGAAACTCCACA+GGG - chr4_3:335541-335560 Msa0623140:intron 50.0%
GCTGCTATATTCACCGTACC+TGG - chr4_3:335629-335648 Msa0623140:intron 50.0%
GCTATATTCACCGTACCTGG+TGG - chr4_3:335632-335651 Msa0623140:intron 50.0%
ATCGTTATCCCCACCAGGTA+CGG + chr4_3:335645-335664 None:intergenic 50.0%
!! GGGGATAACGATGATGGATC+AGG - chr4_3:335653-335672 Msa0623140:intron 50.0%
! GTTGGCTTCTGTCTGTACCT+CGG - chr4_3:335832-335851 Msa0623140:CDS 50.0%
AGAGGCAATAAATGCCACCG+AGG + chr4_3:335852-335871 None:intergenic 50.0%
GCCTCTTCGTACATAGTCGT+CGG - chr4_3:335866-335885 Msa0623140:CDS 50.0%
ACCGACGACTATGTACGAAG+AGG + chr4_3:335870-335889 None:intergenic 50.0%
CTTCGTACATAGTCGTCGGT+CGG - chr4_3:335870-335889 Msa0623140:CDS 50.0%
AGAAAGAGAAGCAGCTAGCC+AGG - chr4_3:336008-336027 Msa0623140:CDS 50.0%
! TGAGTTGGATCCACTTCTCC+TGG + chr4_3:336029-336048 None:intergenic 50.0%
GGAGAAGTGGATCCAACTCA+TGG - chr4_3:336029-336048 Msa0623140:CDS 50.0%
!! AAGACCCTCAATTGGCTCGG+AGG - chr4_3:334423-334442 Msa0623140:intron 55.0%
CAGTCCTCCGAGCCAATTGA+GGG + chr4_3:334430-334449 None:intergenic 55.0%
TCAGTCCTCCGAGCCAATTG+AGG + chr4_3:334431-334450 None:intergenic 55.0%
GCTCGGAGGACTGACAAGAA+AGG - chr4_3:334437-334456 Msa0623140:intron 55.0%
AAAAGGGCAGAGCTGTGACG+TGG - chr4_3:334484-334503 Msa0623140:intron 55.0%
CAGCATTGCATGTGGCAACC+AGG - chr4_3:334567-334586 Msa0623140:CDS 55.0%
ACCAACCAAGGGACGAACTG+AGG - chr4_3:335432-335451 Msa0623140:intron 55.0%
TCCTCAGTTCGTCCCTTGGT+TGG + chr4_3:335436-335455 None:intergenic 55.0%
CTGAGGAAACTCCACAGGGA+AGG - chr4_3:335545-335564 Msa0623140:intron 55.0%
! TGCCACAAACTCGGTGACAG+TGG - chr4_3:335577-335596 Msa0623140:intron 55.0%
CACCACTGTCACCGAGTTTG+TGG + chr4_3:335582-335601 None:intergenic 55.0%
! CACAAACTCGGTGACAGTGG+TGG - chr4_3:335580-335599 Msa0623140:intron 55.0%
CCATCATCGTTATCCCCACC+AGG + chr4_3:335650-335669 None:intergenic 55.0%
CCTGGTGGGGATAACGATGA+TGG - chr4_3:335647-335666 Msa0623140:intron 55.0%
!! GAACGAGTGGGCTGCAATTC+TGG - chr4_3:335895-335914 Msa0623140:CDS 55.0%
AAGCAGCTAGCCAGGAGAAG+TGG - chr4_3:336016-336035 Msa0623140:CDS 55.0%
GGCTTCTGTCTGTACCTCGG+TGG - chr4_3:335835-335854 Msa0623140:CDS 60.0%
CGTACATAGTCGTCGGTCGG+AGG - chr4_3:335873-335892 Msa0623140:CDS 60.0%
! GACGAGAGGGCACGTCGAAG+TGG - chr4_3:334280-334299 Msa0623140:intron 65.0%
TCGTCGGTCGGAGGAACGAG+TGG - chr4_3:335882-335901 Msa0623140:CDS 65.0%
CGTCGGTCGGAGGAACGAGT+GGG - chr4_3:335883-335902 Msa0623140:CDS 65.0%
GCGGACGACGGAGATGGCGA+CGG + chr4_3:333037-333056 None:intergenic 70.0%
GCCATCTCCGTCGTCCGCGA+CGG - chr4_3:333039-333058 Msa0623140:three_prime_UTR 70.0%
GCCGTCGCGGACGACGGAGA+TGG + chr4_3:333043-333062 None:intergenic 75.0%
GACTAGAGCCGGCGCCGTCG+CGG + chr4_3:333056-333075 None:intergenic 75.0%
! TCCGTCGTCCGCGACGGCGC+CGG - chr4_3:333045-333064 Msa0623140:three_prime_UTR 80.0%
! GCCGGCGCCGTCGCGGACGA+CGG + chr4_3:333049-333068 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 332594 336576 332594 ID=Msa0623140;Name=Msa0623140
chr4_3 mRNA 332594 336576 332594 ID=Msa0623140-mRNA-1;Parent=Msa0623140;Name=Msa0623140-mRNA-1;_AED=0.05;_eAED=0.05;_QI=234|1|1|1|0.75|0.6|5|454|593
chr4_3 exon 332594 332765 332594 ID=Msa0623140-mRNA-1:exon:69;Parent=Msa0623140-mRNA-1
chr4_3 exon 332786 333901 332786 ID=Msa0623140-mRNA-1:exon:68;Parent=Msa0623140-mRNA-1
chr4_3 exon 334566 334975 334566 ID=Msa0623140-mRNA-1:exon:67;Parent=Msa0623140-mRNA-1
chr4_3 exon 335701 336213 335701 ID=Msa0623140-mRNA-1:exon:66;Parent=Msa0623140-mRNA-1
chr4_3 exon 336318 336576 336318 ID=Msa0623140-mRNA-1:exon:65;Parent=Msa0623140-mRNA-1
chr4_3 five_prime_UTR 336343 336576 336343 ID=Msa0623140-mRNA-1:five_prime_utr;Parent=Msa0623140-mRNA-1
chr4_3 CDS 336318 336342 336318 ID=Msa0623140-mRNA-1:cds;Parent=Msa0623140-mRNA-1
chr4_3 CDS 335701 336213 335701 ID=Msa0623140-mRNA-1:cds;Parent=Msa0623140-mRNA-1
chr4_3 CDS 334566 334975 334566 ID=Msa0623140-mRNA-1:cds;Parent=Msa0623140-mRNA-1
chr4_3 CDS 333068 333901 333068 ID=Msa0623140-mRNA-1:cds;Parent=Msa0623140-mRNA-1
chr4_3 three_prime_UTR 332786 333067 332786 ID=Msa0623140-mRNA-1:three_prime_utr;Parent=Msa0623140-mRNA-1
chr4_3 three_prime_UTR 332594 332765 332594 ID=Msa0623140-mRNA-1:three_prime_utr;Parent=Msa0623140-mRNA-1
Gene Sequence

>Msa0623140

ATGACCTCCAATTTCAACCTTCCAGGTGGAGCTCAAACTCAAAGAGATTCAGAAAAAGGAACGATGATGACACCAACACAGAACCCACTTTCCGAAATATCTCCGTCGCCATCTCCGTCGTCCGCGACGGCGCCGGCTCTAGTCCTTTCAAACTCAGGAAAACGGATCGATCAAGCAGGGAAGAAGAAGTACGTGAAGCAAGTAACGGGTCGTCACAACGACACAGAGCTTCATCTAGCAGCTCAAAGAGGTGATGTTGGTGCTGTGAGACAGATCCTTCTTGATATTGATTCTCAGATAATGGGTACTTTGAGTGGTGATGATGATGTTGATCTTAACGCTGAGATTGCTGAAGTTCGTGCTCTTGTTGTGAATGAAGAGAATGAACTTGGTGAAACTGCTTTGTTTACTGCTGCTGAAAAGGGTCATCTTGATGTTGTTAAGGAGTTGTTGAAACATTCAACTCTTCAAACTGTTTCTAAAAAGAATCGATCTGGTTTTGATCCTTTGCATATTGCTGCTAGTCAAGGACATCATGCCATTGTTCAGGTTTTACTAGATTATGACCCAAGCTTAAGCAAAACTATTGGTCCATCCAACGCCACTCCACTTATAACAGCAGCGACGAGAGGGCACGTCGAAGTGGTGAATGAACTTCTGTCAAAGGATGGCAGCTTGTTGGAAATTGCAAGATCCAATGGAAAAAATGCTTTGCATTTAGCTGCTCGTCCGGGCCATACAGAAATCGTGAAAGCCTTGCTCAGCAAAGACCCTCAATTGGCTCGGAGGACTGACAAGAAAGGGCAAACTGCATTGCATATGGCTGTAAAAGGGCAGAGCTGTGACGTGGTGAAATTGCTTCTTGATGCAGATGCTGCTATTGTTATGCTTCCTGACAAATTTGGCAACACAGCATTGCATGTGGCAACCAGGAAAAAGCGAGTAGAGATAGTGAATGAGTTGTTACTCCTGCCAGACACAAACGTAAATGCATTAACACGAGACCACAAAACAGCCCTTGACATTGCTGAAAATCTTCCTCTCTCTGAAGAGTCATCAGATATAAAGGACATCCTCTCTCGATATGGGGCACTTAGAGCTAACGAGCTCAACCAACCAAGGGACGAACTGAGGAAAACGGTTACACAAATTAAGAAAGATGTTCACACACAGCTGGAACAAACCAAGAGAACCAACAAAAATGTCCACAATATATCCAAAGAGCTGAGGAAACTCCACAGGGAAGGAATTAACAATGCCACAAACTCGGTGACAGTGGTGGCTGTGTTGTTTGCTACAGTTGCTTTTGCTGCTATATTCACCGTACCTGGTGGGGATAACGATGATGGATCAGGAGTGGTAGCGAGTTATTCTGCTTTTAAAATTTTCTTCATATTCAATGCTATTGCGCTTTTTACATCTTTGGCTGTTGTGGTTGTTCAAATTACCCTTGTTAGAGGTGAAACTAAAGCAGAGAAAAGGGTAGTTGAGGTGATTAACAAGCTCATGTGGTTGGCTTCTGTCTGTACCTCGGTGGCATTTATTGCCTCTTCGTACATAGTCGTCGGTCGGAGGAACGAGTGGGCTGCAATTCTGGTTACAGTTGTTGGAGGAGTGATAATATCCGGAGTTATTGGCACTATGACTTACTATGTAGTGAGGTCTAAGAGAACAAGATCCATGAGGAAGAAAGAGAAGCAGCTAGCCAGGAGAAGTGGATCCAACTCATGGCATCATTCAGAGTTCTCCAATTCCGAGGTTGATCGAATTTATGCGCTCTAA
Protein sequence

>Msa0623140

MTSNFNLPGGAQTQRDSEKGTMMTPTQNPLSEISPSPSPSSATAPALVLSNSGKRIDQAGKKKYVKQVTGRHNDTELHLAAQRGDVGAVRQILLDIDSQIMGTLSGDDDVDLNAEIAEVRALVVNEENELGETALFTAAEKGHLDVVKELLKHSTLQTVSKKNRSGFDPLHIAASQGHHAIVQVLLDYDPSLSKTIGPSNATPLITAATRGHVEVVNELLSKDGSLLEIARSNGKNALHLAARPGHTEIVKALLSKDPQLARRTDKKGQTALHMAVKGQSCDVVKLLLDADAAIVMLPDKFGNTALHVATRKKRVEIVNELLLLPDTNVNALTRDHKTALDIAENLPLSEESSDIKDILSRYGALRANELNQPRDELRKTVTQIKKDVHTQLEQTKRTNKNVHNISKELRKLHREGINNATNSVTVVAVLFATVAFAAIFTVPGGDNDDGSGVVASYSAFKIFFIFNAIALFTSLAVVVVQITLVRGETKAEKRVVEVINKLMWLASVCTSVAFIASSYIVVGRRNEWAAILVTVVGGVIISGVIGTMTYYVVRSKRTRSMRKKEKQLARRSGSNSWHHSEFSNSEVDRIYAL*