AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1409720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1409720 MtrunA17_Chr1g0212971 99.157 593 5 0 1 593 1 593 0.0 1201
Msa1409720 MtrunA17_Chr7g0263471 73.042 549 141 4 46 593 40 582 0.0 791
Msa1409720 MtrunA17_Chr2g0327711 46.209 554 253 9 61 593 15 544 2.55e-147 436
Msa1409720 MtrunA17_Chr4g0030551 47.126 522 237 8 58 563 16 514 2.40e-145 431
Msa1409720 MtrunA17_Chr7g0276681 45.421 546 255 7 61 593 31 546 7.71e-137 409
Msa1409720 MtrunA17_Chr1g0161231 45.436 482 246 7 127 593 52 531 5.60e-128 386
Msa1409720 MtrunA17_Chr7g0263421 42.620 542 273 7 66 593 3 520 2.95e-125 379
Msa1409720 MtrunA17_Chr8g0391711 43.659 481 256 4 127 593 59 538 6.24e-125 378
Msa1409720 MtrunA17_Chr2g0331191 22.951 610 356 14 4 567 69 610 8.96e-27 115
Msa1409720 MtrunA17_Chr2g0331191 25.403 248 184 1 96 343 1 247 1.05e-20 96.3
Msa1409720 MtrunA17_Chr3g0136651 22.707 458 298 12 138 577 8 427 3.84e-25 108
Msa1409720 MtrunA17_Chr2g0331201 28.896 308 202 5 74 367 37 341 1.77e-21 99.0
Msa1409720 MtrunA17_Chr2g0331201 26.949 449 250 15 130 522 137 563 1.68e-20 95.9
Msa1409720 MtrunA17_Chr2g0331201 26.471 238 173 2 107 343 11 247 4.28e-19 91.3
Msa1409720 MtrunA17_Chr8g0361991 25.469 373 214 10 76 445 10 321 1.60e-19 91.7
Msa1409720 MtrunA17_Chr2g0328031 31.707 205 121 4 134 322 14 215 1.99e-19 91.3
Msa1409720 MtrunA17_Chr8g0370361 26.756 299 178 6 169 452 46 318 5.30e-19 90.1
Msa1409720 MtrunA17_Chr8g0367671 23.786 412 245 12 169 543 47 426 1.21e-18 89.0
Msa1409720 MtrunA17_Chr1g0178581 24.565 460 262 15 138 556 15 430 3.83e-18 87.4
Msa1409720 MtrunA17_Chr7g0233331 24.611 321 206 6 138 445 12 309 2.26e-16 81.6
Msa1409720 MtrunA17_Chr5g0439361 25.908 413 253 13 169 562 23 401 3.14e-16 81.3
Msa1409720 MtrunA17_Chr5g0439351 27.128 376 238 11 169 533 338 688 3.67e-16 82.0
Msa1409720 MtrunA17_Chr5g0439161 24.319 477 310 14 128 562 207 674 6.51e-16 81.3
Msa1409720 MtrunA17_Chr8g0369921 23.653 427 245 13 169 556 45 429 7.85e-16 80.1
Msa1409720 MtrunA17_Chr4g0031491 30.288 208 124 7 132 322 13 216 9.13e-16 80.1
Msa1409720 MtrunA17_Chr8g0368621 24.315 292 180 6 169 445 42 307 2.74e-15 77.4
Msa1409720 MtrunA17_Chr3g0100301 24.552 391 245 14 189 542 15 392 2.94e-15 78.2
Msa1409720 MtrunA17_Chr7g0262731 27.917 240 158 6 135 361 9 246 3.95e-15 78.6
Msa1409720 MtrunA17_Chr7g0221531 23.099 355 208 9 169 488 44 368 1.99e-14 75.5
Msa1409720 MtrunA17_Chr8g0354071 23.684 342 198 10 177 480 2 318 2.79e-14 75.1
Msa1409720 MtrunA17_Chr8g0364031 25.000 336 200 10 138 453 13 316 5.79e-14 74.3
Msa1409720 MtrunA17_Chr5g0418551 33.708 178 111 5 132 306 20 193 1.02e-13 71.2
Msa1409720 MtrunA17_Chr7g0263431 71.429 49 13 1 274 321 1 49 1.26e-13 65.5
Msa1409720 MtrunA17_Chr3g0093531 23.684 342 198 10 177 480 2 318 1.90e-13 72.4
Msa1409720 MtrunA17_Chr3g0083331 24.917 301 178 9 169 445 45 321 2.71e-13 72.4
Msa1409720 MtrunA17_Chr8g0353311 21.809 376 234 9 169 509 49 399 4.61e-13 71.6
Msa1409720 MtrunA17_Chr8g0376971 26.183 317 194 9 72 352 77 389 6.24e-13 71.6
Msa1409720 MtrunA17_Chr1g0211241 29.952 207 137 5 120 322 120 322 1.10e-12 70.9
Msa1409720 MtrunA17_Chr3g0122941 28.342 187 125 3 131 309 105 290 1.56e-12 70.5
Msa1409720 MtrunA17_Chr3g0122941 27.208 283 173 9 100 355 108 384 2.92e-12 69.7
Msa1409720 MtrunA17_Chr6g0461061 27.935 247 158 7 90 323 125 364 1.63e-11 67.0
Msa1409720 MtrunA17_Chr5g0410201 25.284 352 185 11 213 544 7 300 1.71e-11 65.9
Msa1409720 MtrunA17_Chr4g0064931 30.319 188 127 4 135 322 40 223 2.05e-11 67.0
Msa1409720 MtrunA17_Chr7g0262761 27.922 154 100 3 169 312 47 199 2.36e-11 63.5
Msa1409720 MtrunA17_Chr4g0073161 30.392 204 98 6 128 294 9 205 3.05e-11 66.2
Msa1409720 MtrunA17_Chr1g0196261 28.947 228 134 8 114 317 198 421 5.70e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1409720 AT3G12360.1 80.684 585 104 4 14 593 10 590 0.0 954
Msa1409720 AT3G12360.2 80.895 581 102 4 18 593 2 578 0.0 950
Msa1409720 AT3G09550.1 71.502 586 148 3 24 593 25 607 0.0 833
Msa1409720 AT3G09550.3 71.502 586 148 3 24 593 25 607 0.0 833
Msa1409720 AT3G09550.2 71.502 586 148 3 24 593 32 614 0.0 832
Msa1409720 AT2G31820.1 43.534 549 271 7 60 593 138 662 8.01e-138 416
Msa1409720 AT1G05640.1 44.404 554 270 7 56 593 96 627 7.62e-136 410
Msa1409720 AT5G60070.1 46.595 558 244 13 61 593 20 548 3.40e-135 405
Msa1409720 AT2G01680.1 43.110 508 273 8 99 593 28 532 2.25e-129 390
Msa1409720 AT1G07710.2 44.565 552 264 5 61 593 15 543 2.44e-129 390
Msa1409720 AT1G07710.1 44.565 552 264 5 61 593 15 543 2.44e-129 390
Msa1409720 AT5G02620.4 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa1409720 AT5G02620.1 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa1409720 AT5G02620.3 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa1409720 AT5G02620.5 41.899 537 248 8 61 556 5 518 1.15e-118 363
Msa1409720 AT5G02620.2 41.899 537 248 8 61 556 5 518 1.15e-118 363
Msa1409720 AT1G10340.1 25.202 496 309 12 111 552 51 538 2.25e-34 138
Msa1409720 AT5G54710.1 27.308 520 284 17 68 552 97 557 1.49e-33 135
Msa1409720 AT5G54710.2 27.308 520 284 17 68 552 142 602 2.33e-33 135
Msa1409720 AT1G10340.2 24.593 492 313 11 111 552 51 534 2.38e-33 135
Msa1409720 AT1G34050.1 26.145 524 294 15 108 554 28 535 6.31e-33 134
Msa1409720 AT2G24600.3 26.892 502 290 18 72 538 70 529 3.55e-32 131
Msa1409720 AT2G24600.4 26.892 502 290 18 72 538 70 529 3.55e-32 131
Msa1409720 AT4G03500.2 26.047 549 280 17 58 522 54 560 2.29e-30 126
Msa1409720 AT4G03500.3 26.047 549 280 17 58 522 54 560 2.29e-30 126
Msa1409720 AT4G03500.1 26.047 549 280 17 58 522 77 583 2.43e-30 126
Msa1409720 AT1G03670.1 25.581 516 328 9 74 562 107 593 2.56e-26 114
Msa1409720 AT4G03490.3 27.674 430 267 12 133 551 180 576 5.97e-25 110
Msa1409720 AT4G03490.5 27.830 424 262 12 133 545 170 560 7.94e-25 109
Msa1409720 AT4G03490.4 27.830 424 262 12 133 545 180 570 8.14e-25 109
Msa1409720 AT4G03460.2 26.406 409 256 8 128 522 158 535 8.35e-24 106
Msa1409720 AT4G03460.1 26.406 409 256 8 128 522 190 567 9.03e-24 106
Msa1409720 AT4G03490.2 24.915 586 332 18 65 574 9 562 1.03e-23 106
Msa1409720 AT5G50140.1 22.913 515 297 16 126 563 22 513 1.05e-23 105
Msa1409720 AT4G03490.1 25.277 542 318 15 65 551 19 528 2.42e-23 105
Msa1409720 AT5G54700.2 28.435 313 159 9 223 523 20 279 1.39e-22 99.8
Msa1409720 AT4G10720.1 24.096 415 258 12 169 559 40 421 3.32e-22 100
Msa1409720 AT5G54700.1 27.809 356 182 12 202 523 2 316 6.75e-22 99.8
Msa1409720 AT4G10720.2 25.076 327 210 6 169 477 40 349 1.96e-21 97.4
Msa1409720 AT4G14390.2 24.722 449 281 11 128 556 226 637 2.16e-20 95.9
Msa1409720 AT4G14390.4 24.722 449 281 11 128 556 245 656 2.63e-20 95.5
Msa1409720 AT4G14390.1 24.889 450 280 12 128 556 245 657 2.98e-20 95.5
Msa1409720 AT4G03480.1 26.076 395 232 9 118 482 295 659 1.57e-19 93.2
Msa1409720 AT4G03480.2 26.076 395 232 9 118 482 292 656 1.65e-19 93.2
Msa1409720 AT4G05040.3 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1409720 AT4G05040.2 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1409720 AT4G05040.5 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1409720 AT4G05040.4 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1409720 AT4G05040.1 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1409720 AT4G05040.6 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1409720 AT4G05040.7 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1409720 AT5G15500.2 23.427 461 288 15 130 559 1 427 2.70e-18 88.2
Msa1409720 AT2G24600.2 26.420 405 232 14 72 450 70 434 1.75e-17 86.3
Msa1409720 AT2G24600.1 26.420 405 232 14 72 450 70 434 1.75e-17 86.3
Msa1409720 AT4G03450.2 24.505 555 306 17 58 528 45 570 1.81e-17 86.7
Msa1409720 AT5G51160.1 25.000 352 198 8 157 478 38 353 2.38e-17 85.5
Msa1409720 AT4G03450.1 24.505 555 306 17 58 528 45 570 5.28e-17 85.1
Msa1409720 AT4G03470.1 26.044 503 294 18 77 542 157 618 1.34e-16 84.0
Msa1409720 AT3G04140.1 27.622 286 160 8 100 342 105 386 2.92e-16 82.8
Msa1409720 AT1G14500.1 22.769 325 215 5 169 475 40 346 3.84e-15 78.6
Msa1409720 AT2G03430.1 34.659 176 106 5 135 306 16 186 8.50e-15 74.7
Msa1409720 AT4G14390.3 26.748 329 195 8 128 443 226 521 4.92e-13 72.4
Msa1409720 AT5G54620.1 29.570 186 119 2 169 342 40 225 8.08e-13 71.2
Msa1409720 AT5G54610.1 27.717 184 123 2 169 342 40 223 8.13e-13 71.2
Msa1409720 AT3G18670.1 23.111 450 296 12 123 530 113 554 1.09e-12 71.2
Msa1409720 AT2G28840.1 27.273 209 129 7 132 322 12 215 2.01e-12 70.1
Msa1409720 AT4G03440.1 23.126 467 308 12 104 550 258 693 3.95e-12 69.7
Msa1409720 AT4G03440.2 23.126 467 308 12 104 550 251 686 4.36e-12 69.7
Msa1409720 AT4G03440.3 23.126 467 308 12 104 550 251 686 4.36e-12 69.7
Msa1409720 AT4G14400.2 23.242 512 310 20 75 538 71 547 4.01e-11 66.2
Msa1409720 AT4G14400.3 23.242 512 310 20 75 538 71 547 4.01e-11 66.2
Msa1409720 AT3G18670.2 23.684 304 183 7 236 530 346 609 4.70e-11 66.2
Msa1409720 AT4G14400.1 23.242 512 310 20 75 538 137 613 5.68e-11 65.9

Find 168 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTGCATATGGCTGTAAA+AGG 0.222840 tig0023536:-55555 Msa1409720:CDS
TTATCCTTTCCAACGTAATT+TGG 0.223179 tig0023536:+53910 None:intergenic
TGATATTGATTCTCAGATAA+TGG 0.227356 tig0023536:-56844 Msa1409720:CDS
TAAGCTGCTGTTGTTGTTAT+AGG 0.245673 tig0023536:-53617 Msa1409720:three_prime_UTR
AAGAATCATCATAATAATTT+AGG 0.249295 tig0023536:+57311 None:intergenic
GTGGAGTTTCCTCAGCTCTT+TGG 0.250605 tig0023536:+54485 None:intergenic
GTTATGCTTCCTGACAAATT+TGG 0.259995 tig0023536:-55483 Msa1409720:CDS
GAAAGGAAAGAGGGAGGAAA+TGG 0.261658 tig0023536:+57426 None:intergenic
GAACGAGTGGGCTGCAATTC+TGG 0.267500 tig0023536:-54127 Msa1409720:CDS
ACCTCCAATTTCAACCTTCC+AGG 0.283929 tig0023536:-57227 Msa1409720:intron
ACTCAAAGAGATTCAGAAAA+AGG 0.285730 tig0023536:-57089 Msa1409720:CDS
AAGGACATCCTCTCTCGATA+TGG 0.290107 tig0023536:-54636 Msa1409720:CDS
CCAACCTGGAAGGTTGAAAT+TGG 0.303178 tig0023536:+57223 None:intergenic
GTTATAGGATCATAACAAAT+TGG 0.307029 tig0023536:-53602 Msa1409720:three_prime_UTR
TTCATTTGCAGCCATTGTTC+AGG 0.330505 tig0023536:-55838 Msa1409720:intron
CTGCTTCTCTTTCTTCCTCA+TGG 0.331730 tig0023536:+54023 None:intergenic
GCAATTCTGGTTACAGTTGT+TGG 0.335512 tig0023536:-54114 Msa1409720:CDS
GATATTGATTCTCAGATAAT+GGG 0.350929 tig0023536:-56843 Msa1409720:CDS
AATTCGATCAACCTCGGAAT+TGG 0.351942 tig0023536:+53954 None:intergenic
AAAGGAAAGAGGGAGGAAAT+GGG 0.356439 tig0023536:+57427 None:intergenic
TGAAGAGTCATCAGATATAA+AGG 0.366092 tig0023536:-54655 Msa1409720:CDS
TAATAAGTGTTGGCTTTGAT+TGG 0.372963 tig0023536:+57370 None:intergenic
TTTGTTTACTGCTGCTGAAA+AGG 0.377535 tig0023536:-56724 Msa1409720:CDS
AGATGGCGACGGAGATATTT+CGG 0.380641 tig0023536:+57034 None:intergenic
GTTCAAATTACCCTTGTTAG+AGG 0.385400 tig0023536:-54264 Msa1409720:CDS
GTGATAATATCCGGAGTTAT+TGG 0.388823 tig0023536:-54087 Msa1409720:CDS
GTGAAACTAAAGCAGAGAAA+AGG 0.392404 tig0023536:-54242 Msa1409720:CDS
TTTGCATTTAGCTGCTCGTC+CGG 0.403120 tig0023536:-55655 Msa1409720:CDS
GTCAAAGGATGGCAGCTTGT+TGG 0.404723 tig0023536:-55706 Msa1409720:CDS
GGGTCATCTTGATGTTGTTA+AGG 0.408314 tig0023536:-56703 Msa1409720:CDS
AGAAAGAGAAGCAGCTAGCC+AGG 0.411228 tig0023536:-54014 Msa1409720:CDS
AGGACATCCTCTCTCGATAT+GGG 0.415929 tig0023536:-54635 Msa1409720:CDS
CACCACTGTCACCGAGTTTG+TGG 0.435710 tig0023536:+54443 None:intergenic
CTCGTCGCTGCTGTTATAAG+TGG 0.455896 tig0023536:+55759 None:intergenic
TCCGTCGTCCGCGACGGCGC+CGG 0.456322 tig0023536:-57012 Msa1409720:CDS
ATGGGAAAGTCGTGAGAAAG+AGG 0.462631 tig0023536:+57445 None:intergenic
AGGCTTTCACGATTTCTGTA+TGG 0.466846 tig0023536:+55631 None:intergenic
TTGCATTTAGCTGCTCGTCC+GGG 0.469773 tig0023536:-55654 Msa1409720:CDS
GCTGCTATATTCACCGTACC+TGG 0.472210 tig0023536:-54393 Msa1409720:CDS
TCCTCAGTTCGTCCCTTGGT+TGG 0.473921 tig0023536:+54589 None:intergenic
GAGTGGAAGGAAAGGAAAGA+GGG 0.474346 tig0023536:+57417 None:intergenic
CATTTACGTTTGTGTCTGGC+AGG 0.475438 tig0023536:+54733 None:intergenic
TTGTTTACTGCTGCTGAAAA+GGG 0.479987 tig0023536:-56723 Msa1409720:CDS
GCTCAGCAAAGACCCTCAAT+TGG 0.481998 tig0023536:-55607 Msa1409720:CDS
CTTTAGTTTCACCTCTAACA+AGG 0.482809 tig0023536:+54253 None:intergenic
CCAAGCTTAAGCAAAACTAT+TGG 0.486154 tig0023536:-55798 Msa1409720:CDS
GAAAAGATGAGTGGAAGGAA+AGG 0.490183 tig0023536:+57409 None:intergenic
TGAGTTGGATCCACTTCTCC+TGG 0.491866 tig0023536:+53996 None:intergenic
GGGGATAACGATGATGGATC+AGG 0.492044 tig0023536:-54369 Msa1409720:CDS
CAGTCCTCCGAGCCAATTGA+GGG 0.492228 tig0023536:+55595 None:intergenic
TCCTTTCAAACTCAGGAAAA+CGG 0.494021 tig0023536:-56983 Msa1409720:CDS
TGAGTGGAAGGAAAGGAAAG+AGG 0.495388 tig0023536:+57416 None:intergenic
TGAAACTAAAGCAGAGAAAA+GGG 0.501105 tig0023536:-54241 Msa1409720:CDS
AGGGCAAACTGCATTGCATA+TGG 0.501695 tig0023536:-55565 Msa1409720:CDS
TCAGCTCTTTGGATATATTG+TGG 0.502735 tig0023536:+54496 None:intergenic
GGTCATGATAGAAGAGTAGA+GGG 0.503098 tig0023536:+57247 None:intergenic
ATCGTTATCCCCACCAGGTA+CGG 0.503271 tig0023536:+54380 None:intergenic
TTGAGGGTCTTTGCTGAGCA+AGG 0.503619 tig0023536:+55611 None:intergenic
AATGCATTTACGTTTGTGTC+TGG 0.503703 tig0023536:+54729 None:intergenic
TTGGAAATTGCAAGATCCAA+TGG 0.508338 tig0023536:-55687 Msa1409720:CDS
AAGTACGTGAAGCAAGTAAC+GGG 0.508570 tig0023536:-56939 Msa1409720:CDS
GCTCGGAGGACTGACAAGAA+AGG 0.509842 tig0023536:-55585 Msa1409720:CDS
AAAGAGAAAAGATGAGTGGA+AGG 0.510130 tig0023536:+57404 None:intergenic
GACGGAGATATTTCGGAAAG+TGG 0.514348 tig0023536:+57041 None:intergenic
CTCGGAGGACTGACAAGAAA+GGG 0.519611 tig0023536:-55584 Msa1409720:CDS
TTCACGATTTCTGTATGGCC+CGG 0.525638 tig0023536:+55636 None:intergenic
TATGCATTTGTATAAATCAC+AGG 0.525663 tig0023536:-53520 Msa1409720:three_prime_UTR
GTTGGAGGAGTGATAATATC+CGG 0.527167 tig0023536:-54096 Msa1409720:CDS
GCCGGCGCCGTCGCGGACGA+CGG 0.529146 tig0023536:+57011 None:intergenic
CATTGCATATGGCTGTAAAA+GGG 0.531587 tig0023536:-55554 Msa1409720:CDS
AAGACCAAATTACGTTGGAA+AGG 0.532286 tig0023536:-53914 Msa1409720:three_prime_UTR
GAAAGATGTTCACACACAGC+TGG 0.535788 tig0023536:-54547 Msa1409720:CDS
CCTGGTGGGGATAACGATGA+TGG 0.535860 tig0023536:-54375 Msa1409720:CDS
GCCATCTCCGTCGTCCGCGA+CGG 0.536247 tig0023536:-57018 Msa1409720:CDS
GCTGTGTTGCCAAATTTGTC+AGG 0.537540 tig0023536:+55474 None:intergenic
GGAAAACGGATCGATCAAGC+AGG 0.537963 tig0023536:-56969 Msa1409720:CDS
CTATATTCACCGTACCTGGT+GGG 0.538227 tig0023536:-54389 Msa1409720:CDS
TCATAATAATTTAGGAAGGA+AGG 0.539269 tig0023536:+57319 None:intergenic
CAGCATTGCATGTGGCAACC+AGG 0.541967 tig0023536:-55455 Msa1409720:CDS
AAGCAGCTAGCCAGGAGAAG+TGG 0.542236 tig0023536:-54006 Msa1409720:CDS
TCAGTCCTCCGAGCCAATTG+AGG 0.545108 tig0023536:+55594 None:intergenic
TCGGAAAGTGGGTTCTGTGT+TGG 0.547229 tig0023536:+57053 None:intergenic
GACTAGAGCCGGCGCCGTCG+CGG 0.548417 tig0023536:+57004 None:intergenic
GAAAGTCGTGAGAAAGAGGG+AGG 0.548432 tig0023536:+57449 None:intergenic
TTGCATATTGCTGCTAGTCA+AGG 0.549825 tig0023536:-56618 Msa1409720:CDS
CAGATAATGGGTACTTTGAG+TGG 0.549949 tig0023536:-56831 Msa1409720:CDS
TGGAAGGAAAGGAAAGAGGG+AGG 0.550810 tig0023536:+57420 None:intergenic
AAGTCGTGAGAAAGAGGGAG+GGG 0.550978 tig0023536:+57451 None:intergenic
TGGGAAAGTCGTGAGAAAGA+GGG 0.551251 tig0023536:+57446 None:intergenic
CTCTGAATGATGCCATGAGT+TGG 0.551504 tig0023536:+53981 None:intergenic
AAGAGCTGAGGAAACTCCAC+AGG 0.552962 tig0023536:-54482 Msa1409720:CDS
ATCTATGTAAACTCTTGAAG+TGG 0.553547 tig0023536:+53683 None:intergenic
ATCTATGTAAACTCTTGAAG+TGG 0.553547 tig0023536:+53853 None:intergenic
ATTCTGGTTACAGTTGTTGG+AGG 0.554005 tig0023536:-54111 Msa1409720:CDS
GATTAACAAGCTCATGTGGT+TGG 0.554304 tig0023536:-54208 Msa1409720:CDS
AAGGGACGAACTGAGGAAAA+CGG 0.554349 tig0023536:-54583 Msa1409720:CDS
AGGTCATGATAGAAGAGTAG+AGG 0.556929 tig0023536:+57246 None:intergenic
AAAGTCGTGAGAAAGAGGGA+GGG 0.557510 tig0023536:+57450 None:intergenic
ACCTGGAAGGTTGAAATTGG+AGG 0.558297 tig0023536:+57226 None:intergenic
GCCGTCGCGGACGACGGAGA+TGG 0.559929 tig0023536:+57017 None:intergenic
CCATCATCGTTATCCCCACC+AGG 0.564948 tig0023536:+54375 None:intergenic
TTTAGTTTCACCTCTAACAA+GGG 0.565107 tig0023536:+54254 None:intergenic
GTTTGAAGACCAAATTACGT+TGG 0.566835 tig0023536:-53919 Msa1409720:three_prime_UTR
GAGTTTGAAAGGACTAGAGC+CGG 0.567456 tig0023536:+56993 None:intergenic
GCAAAGACCCTCAATTGGCT+CGG 0.569896 tig0023536:-55602 Msa1409720:CDS
GCTCTAGTCCTTTCAAACTC+AGG 0.572097 tig0023536:-56990 Msa1409720:CDS
CATTGTTAATTCCTTCCCTG+TGG 0.572226 tig0023536:+54466 None:intergenic
AGCAGAGAAAAGGGTAGTTG+AGG 0.573019 tig0023536:-54232 Msa1409720:CDS
TCGTCGGTCGGAGGAACGAG+TGG 0.574175 tig0023536:-54140 Msa1409720:CDS
CGCTGCTGTTATAAGTGGAG+TGG 0.575162 tig0023536:+55764 None:intergenic
GCTGCTAGTCAAGGACATCA+TGG 0.575482 tig0023536:-56609 Msa1409720:intron
TGATAAAGAGAAAAGATGAG+TGG 0.575965 tig0023536:+57400 None:intergenic
ATAAGTGGAGTGGCGTTGGA+TGG 0.578501 tig0023536:+55774 None:intergenic
GGAAGGAAGGATGAAAGTGA+AGG 0.582860 tig0023536:+57332 None:intergenic
GCAGCTCAAAGAGGTGATGT+TGG 0.583391 tig0023536:-56888 Msa1409720:CDS
GTGAATGAAGAGAATGAACT+TGG 0.587406 tig0023536:-56756 Msa1409720:CDS
TCTGAGAATCAATATCAAGA+AGG 0.587872 tig0023536:+56850 None:intergenic
ACGGAGATATTTCGGAAAGT+GGG 0.587917 tig0023536:+57042 None:intergenic
TGTTATAAGTGGAGTGGCGT+TGG 0.592828 tig0023536:+55770 None:intergenic
TTCAGAGTTCTCCAATTCCG+AGG 0.592884 tig0023536:-53965 Msa1409720:CDS
GCATGAGTCATAATAAGTGT+TGG 0.594195 tig0023536:+57360 None:intergenic
ATCATCATAATAATTTAGGA+AGG 0.594731 tig0023536:+57315 None:intergenic
GCTATATTCACCGTACCTGG+TGG 0.598478 tig0023536:-54390 Msa1409720:CDS
ATCTAGTAAAACCTGAACAA+TGG 0.600510 tig0023536:+55827 None:intergenic
GCCTCTTCGTACATAGTCGT+CGG 0.600824 tig0023536:-54156 Msa1409720:CDS
CGCATAAATTCGATCAACCT+CGG 0.601587 tig0023536:+53948 None:intergenic
CGTACATAGTCGTCGGTCGG+AGG 0.602499 tig0023536:-54149 Msa1409720:CDS
GTTGGCTTCTGTCTGTACCT+CGG 0.602568 tig0023536:-54190 Msa1409720:CDS
CTGAGGAAACTCCACAGGGA+AGG 0.603249 tig0023536:-54477 Msa1409720:CDS
TATATTCACCGTACCTGGTG+GGG 0.603908 tig0023536:-54388 Msa1409720:CDS
GAAGTACGTGAAGCAAGTAA+CGG 0.604371 tig0023536:-56940 Msa1409720:CDS
GGAGAAGTGGATCCAACTCA+TGG 0.605497 tig0023536:-53993 Msa1409720:CDS
AGAAGAAGAACCAACCTGGA+AGG 0.609441 tig0023536:+57213 None:intergenic
GACGAGAGGGCACGTCGAAG+TGG 0.609993 tig0023536:-55742 Msa1409720:CDS
GGTGAATGAACTTCTGTCAA+AGG 0.612992 tig0023536:-55721 Msa1409720:CDS
TTATAACAGCAGCGACGAGA+GGG 0.613377 tig0023536:-55755 Msa1409720:CDS
AATGAACTTCTGTCAAAGGA+TGG 0.613527 tig0023536:-55717 Msa1409720:CDS
TCTGATGACTCTTCAGAGAG+AGG 0.615975 tig0023536:+54663 None:intergenic
AATTAACAATGCCACAAACT+CGG 0.616650 tig0023536:-54454 Msa1409720:CDS
CGTCGGTCGGAGGAACGAGT+GGG 0.619749 tig0023536:-54139 Msa1409720:CDS
ACCAACCAAGGGACGAACTG+AGG 0.620407 tig0023536:-54590 Msa1409720:CDS
CTAACGAGCTCAACCAACCA+AGG 0.621035 tig0023536:-54602 Msa1409720:CDS
CTTCATCTAGCAGCTCAAAG+AGG 0.623399 tig0023536:-56897 Msa1409720:CDS
CTTCGTACATAGTCGTCGGT+CGG 0.624785 tig0023536:-54152 Msa1409720:CDS
CTTATAACAGCAGCGACGAG+AGG 0.631608 tig0023536:-55756 Msa1409720:CDS
TGCCACAAACTCGGTGACAG+TGG 0.632510 tig0023536:-54445 Msa1409720:CDS
AGTCATAGTGCCAATAACTC+CGG 0.636725 tig0023536:+54077 None:intergenic
TGGCAACACAGCATTGCATG+TGG 0.637663 tig0023536:-55463 Msa1409720:CDS
GGACATCCTCTCTCGATATG+GGG 0.638757 tig0023536:-54634 Msa1409720:CDS
TGACTAGCAGCAATATGCAA+AGG 0.639675 tig0023536:+56621 None:intergenic
ACAATATATCCAAAGAGCTG+AGG 0.644642 tig0023536:-54494 Msa1409720:CDS
AAGACCCTCAATTGGCTCGG+AGG 0.651684 tig0023536:-55599 Msa1409720:CDS
GCGGACGACGGAGATGGCGA+CGG 0.652416 tig0023536:+57023 None:intergenic
GGCTTCTGTCTGTACCTCGG+TGG 0.655940 tig0023536:-54187 Msa1409720:CDS
GAAAACGGATCGATCAAGCA+GGG 0.663000 tig0023536:-56968 Msa1409720:CDS
TAACGATGATGGATCAGGAG+TGG 0.663853 tig0023536:-54364 Msa1409720:CDS
TAAGTGCCCCATATCGAGAG+AGG 0.665007 tig0023536:+54628 None:intergenic
AGGTGATTAACAAGCTCATG+TGG 0.671742 tig0023536:-54212 Msa1409720:CDS
ACCGACGACTATGTACGAAG+AGG 0.681066 tig0023536:+54155 None:intergenic
CACAAACTCGGTGACAGTGG+TGG 0.691648 tig0023536:-54442 Msa1409720:CDS
TAACGAGCTCAACCAACCAA+GGG 0.693605 tig0023536:-54601 Msa1409720:CDS
CTATGACTTACTATGTAGTG+AGG 0.708286 tig0023536:-54062 Msa1409720:CDS
CTAAGAGAACAAGATCCATG+AGG 0.720939 tig0023536:-54038 Msa1409720:CDS
AGAGCTGAGGAAACTCCACA+GGG 0.730372 tig0023536:-54481 Msa1409720:CDS
AAAAGGGCAGAGCTGTGACG+TGG 0.742175 tig0023536:-55538 Msa1409720:CDS
GTCATGATAGAAGAGTAGAG+GGG 0.743790 tig0023536:+57248 None:intergenic
AGAGGCAATAAATGCCACCG+AGG 0.784742 tig0023536:+54173 None:intergenic
GATTTGTGAGAAGAATAACA+CGG 0.801731 tig0023536:-53493 Msa1409720:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTGTATTTTTTTTTTT+TGG - tig0023536:54622-54641 Msa1409720:CDS 10.0%
!! AAGAATCATCATAATAATTT+AGG + tig0023536:53593-53612 None:intergenic 15.0%
!!! TATTTTGCTTTTTGTAAAAA+TGG - tig0023536:54404-54423 Msa1409720:CDS 15.0%
!!! ATTTTGCTTTTTGTAAAAAT+GGG - tig0023536:54405-54424 Msa1409720:CDS 15.0%
!!! TTTTTTTAAGAAGCTATTAA+TGG - tig0023536:54832-54851 Msa1409720:intron 15.0%
!! AAAAATTAAAAGAAAACAAG+AGG + tig0023536:56031-56050 None:intergenic 15.0%
!!! AATATTAGATTTTTCATTCA+AGG + tig0023536:56104-56123 None:intergenic 15.0%
!! ATCATCATAATAATTTAGGA+AGG + tig0023536:53589-53608 None:intergenic 20.0%
!!! TATTTTGAATGAATTGACTT+TGG - tig0023536:54439-54458 Msa1409720:CDS 20.0%
!!! TTTTTTTTGGAACATATTCT+TGG - tig0023536:54635-54654 Msa1409720:CDS 20.0%
!! AATTTAATCAGATATACTTC+TGG + tig0023536:54720-54739 None:intergenic 20.0%
!! TCATAATTGAGTTTCTTAAT+TGG + tig0023536:54797-54816 None:intergenic 20.0%
!! TAATACTACTACTACAAATA+TGG + tig0023536:55605-55624 None:intergenic 20.0%
!! GCAACTACTTAAATAAATAA+AGG + tig0023536:55833-55852 None:intergenic 20.0%
!!! TGTTATTTTGCTTTGAAAAT+TGG - tig0023536:55907-55926 Msa1409720:intron 20.0%
! TCATAATAATTTAGGAAGGA+AGG + tig0023536:53585-53604 None:intergenic 25.0%
! TTACAGTGAAGTAAAAAGAT+GGG + tig0023536:53723-53742 None:intergenic 25.0%
! TTTACAGTGAAGTAAAAAGA+TGG + tig0023536:53724-53743 None:intergenic 25.0%
!!! TGATATTGATTCTCAGATAA+TGG - tig0023536:54057-54076 Msa1409720:CDS 25.0%
!!! GATATTGATTCTCAGATAAT+GGG - tig0023536:54058-54077 Msa1409720:CDS 25.0%
! AACATCAAAACAAAGGAAAA+AGG + tig0023536:54365-54384 None:intergenic 25.0%
!!! TTTTTCCTTTGTTTTGATGT+TGG - tig0023536:54364-54383 Msa1409720:CDS 25.0%
!!! TTTTCCTTTGTTTTGATGTT+GGG - tig0023536:54365-54384 Msa1409720:CDS 25.0%
! AAGGTAAAACGATTTATGTT+TGG - tig0023536:54476-54495 Msa1409720:CDS 25.0%
! AGGTAAAACGATTTATGTTT+GGG - tig0023536:54477-54496 Msa1409720:CDS 25.0%
! TTGAGTATTTATCTTGCTTA+AGG - tig0023536:54768-54787 Msa1409720:intron 25.0%
! CTCAATTATGATGCTATTAA+TGG - tig0023536:54806-54825 Msa1409720:intron 25.0%
! ATTAACTTACTCGAACAAAT+CGG - tig0023536:55555-55574 Msa1409720:CDS 25.0%
! TTTAAGTAGTTGCAAGTATA+AGG - tig0023536:55840-55859 Msa1409720:intron 25.0%
! AAACATGTTATATGAGACAA+GGG - tig0023536:55973-55992 Msa1409720:intron 25.0%
!! TCAATTTTGTCTAGAAGTTT+TGG - tig0023536:56004-56023 Msa1409720:intron 25.0%
!! CAATTTTGTCTAGAAGTTTT+GGG - tig0023536:56005-56024 Msa1409720:intron 25.0%
!!! ATTGTTGTTTCTCAATTTCT+TGG - tig0023536:56121-56140 Msa1409720:intron 25.0%
!! TATATTGTGGACATTTTTGT+TGG + tig0023536:56395-56414 None:intergenic 25.0%
! GTTATAGGATCATAACAAAT+TGG - tig0023536:57299-57318 Msa1409720:five_prime_UTR 25.0%
! TATGCATTTGTATAAATCAC+AGG - tig0023536:57381-57400 Msa1409720:five_prime_UTR 25.0%
TGATAAAGAGAAAAGATGAG+TGG + tig0023536:53504-53523 None:intergenic 30.0%
!! TAATAAGTGTTGGCTTTGAT+TGG + tig0023536:53534-53553 None:intergenic 30.0%
TACAGTGAAGTAAAAAGATG+GGG + tig0023536:53722-53741 None:intergenic 30.0%
ACTCAAAGAGATTCAGAAAA+AGG - tig0023536:53812-53831 Msa1409720:three_prime_UTR 30.0%
TCTGAGAATCAATATCAAGA+AGG + tig0023536:54054-54073 None:intergenic 30.0%
! GTTTCTAAAAAGAATCGATC+TGG - tig0023536:54250-54269 Msa1409720:CDS 30.0%
AAATCCCAACATCAAAACAA+AGG + tig0023536:54372-54391 None:intergenic 30.0%
!!! TTGTTTTGATGTTGGGATTT+TGG - tig0023536:54372-54391 Msa1409720:CDS 30.0%
!! TTTGGCTAAAATTGAGTTGA+AGG - tig0023536:54457-54476 Msa1409720:CDS 30.0%
ACATAGCATTAACTCTACTA+CGG + tig0023536:54903-54922 None:intergenic 30.0%
AATAAGACAACGTTGATGTT+TGG + tig0023536:54937-54956 None:intergenic 30.0%
! AACATCAACGTTGTCTTATT+TGG - tig0023536:54937-54956 Msa1409720:intron 30.0%
AGATTACTACAAAGAACAGA+AGG + tig0023536:55033-55052 None:intergenic 30.0%
ATCTAGTAAAACCTGAACAA+TGG + tig0023536:55077-55096 None:intergenic 30.0%
!! CAATAGTTTTGCTTAAGCTT+GGG + tig0023536:55105-55124 None:intergenic 30.0%
!!! ATGCAAAGCATTTTTTCCAT+TGG + tig0023536:55233-55252 None:intergenic 30.0%
TCATCACTATATGTACTGTA+TGG + tig0023536:55525-55544 None:intergenic 30.0%
TTAACTTACTCGAACAAATC+GGG - tig0023536:55556-55575 Msa1409720:CDS 30.0%
AAGTCTCCAATATTGAAAAC+AGG - tig0023536:55642-55661 Msa1409720:CDS 30.0%
! TAGCTACCTGTTTTCAATAT+TGG + tig0023536:55651-55670 None:intergenic 30.0%
GTGCACACTACTTAAAAAAA+TGG + tig0023536:55943-55962 None:intergenic 30.0%
GAAACATGTTATATGAGACA+AGG - tig0023536:55972-55991 Msa1409720:intron 30.0%
! AAGATTTTCAGCAATGTCAA+GGG + tig0023536:56218-56237 None:intergenic 30.0%
TGAAGAGTCATCAGATATAA+AGG - tig0023536:56246-56265 Msa1409720:intron 30.0%
AATTAACAATGCCACAAACT+CGG - tig0023536:56447-56466 Msa1409720:intron 30.0%
!! TATTGCGCTTTTTACATCTT+TGG - tig0023536:56603-56622 Msa1409720:intron 30.0%
! TTTAGTTTCACCTCTAACAA+GGG + tig0023536:56650-56669 None:intergenic 30.0%
TGAAACTAAAGCAGAGAAAA+GGG - tig0023536:56660-56679 Msa1409720:CDS 30.0%
TTATCCTTTCCAACGTAATT+TGG + tig0023536:56994-57013 None:intergenic 30.0%
ATCTATGTAAACTCTTGAAG+TGG + tig0023536:57051-57070 None:intergenic 30.0%
ATCTATGTAAACTCTTGAAG+TGG + tig0023536:57051-57070 None:intergenic 30.0%
ATCTATGTAAACTCTTGAAG+TGG + tig0023536:57051-57070 None:intergenic 30.0%
GATTTGTGAGAAGAATAACA+CGG - tig0023536:57408-57427 Msa1409720:five_prime_UTR 30.0%
AAAGAGAAAAGATGAGTGGA+AGG + tig0023536:53500-53519 None:intergenic 35.0%
GCATGAGTCATAATAAGTGT+TGG + tig0023536:53544-53563 None:intergenic 35.0%
ACAGTGAAGTAAAAAGATGG+GGG + tig0023536:53721-53740 None:intergenic 35.0%
! TACGCTTTTACGTTTATTGC+AGG - tig0023536:53779-53798 Msa1409720:intron 35.0%
TCCTTTCAAACTCAGGAAAA+CGG - tig0023536:53918-53937 Msa1409720:three_prime_UTR 35.0%
GTGAATGAAGAGAATGAACT+TGG - tig0023536:54145-54164 Msa1409720:CDS 35.0%
TTTGTTTACTGCTGCTGAAA+AGG - tig0023536:54177-54196 Msa1409720:CDS 35.0%
TTGTTTACTGCTGCTGAAAA+GGG - tig0023536:54178-54197 Msa1409720:CDS 35.0%
GCTTCAAGTAGAATCAGTTA+TGG - tig0023536:54575-54594 Msa1409720:CDS 35.0%
TCAGTTATGGAGACAAAATC+AGG - tig0023536:54588-54607 Msa1409720:CDS 35.0%
CTTGAGTAGCTTCAATTTGT+GGG - tig0023536:54976-54995 Msa1409720:intron 35.0%
! TAGCTTCAATTTGTGGGTTA+TGG - tig0023536:54982-55001 Msa1409720:intron 35.0%
!! CCAATAGTTTTGCTTAAGCT+TGG + tig0023536:55106-55125 None:intergenic 35.0%
CCAAGCTTAAGCAAAACTAT+TGG - tig0023536:55103-55122 Msa1409720:intron 35.0%
AATGAACTTCTGTCAAAGGA+TGG - tig0023536:55184-55203 Msa1409720:intron 35.0%
TTGGAAATTGCAAGATCCAA+TGG - tig0023536:55214-55233 Msa1409720:intron 35.0%
CATTGCATATGGCTGTAAAA+GGG - tig0023536:55347-55366 Msa1409720:intron 35.0%
GTTATGCTTCCTGACAAATT+TGG - tig0023536:55418-55437 Msa1409720:intron 35.0%
TGCTACAAAAAATGCAAACG+AGG - tig0023536:55675-55694 Msa1409720:CDS 35.0%
AAGTAGTTGCAAGTATAAGG+TGG - tig0023536:55843-55862 Msa1409720:intron 35.0%
ACATGCTCAAAAGTAACTAG+GGG + tig0023536:56074-56093 None:intergenic 35.0%
GACATGCTCAAAAGTAACTA+GGG + tig0023536:56075-56094 None:intergenic 35.0%
TGACATGCTCAAAAGTAACT+AGG + tig0023536:56076-56095 None:intergenic 35.0%
AATGCATTTACGTTTGTGTC+TGG + tig0023536:56175-56194 None:intergenic 35.0%
! GAAGATTTTCAGCAATGTCA+AGG + tig0023536:56219-56238 None:intergenic 35.0%
!!! GACATTTTTGTTGGTTCTCT+TGG + tig0023536:56386-56405 None:intergenic 35.0%
TCAGCTCTTTGGATATATTG+TGG + tig0023536:56408-56427 None:intergenic 35.0%
ACAATATATCCAAAGAGCTG+AGG - tig0023536:56407-56426 Msa1409720:intron 35.0%
! TTTTACATCTTTGGCTGTTG+TGG - tig0023536:56612-56631 Msa1409720:CDS 35.0%
GTTCAAATTACCCTTGTTAG+AGG - tig0023536:56637-56656 Msa1409720:CDS 35.0%
! CTTTAGTTTCACCTCTAACA+AGG + tig0023536:56651-56670 None:intergenic 35.0%
GTGAAACTAAAGCAGAGAAA+AGG - tig0023536:56659-56678 Msa1409720:CDS 35.0%
GTGATAATATCCGGAGTTAT+TGG - tig0023536:56814-56833 Msa1409720:CDS 35.0%
CTATGACTTACTATGTAGTG+AGG - tig0023536:56839-56858 Msa1409720:CDS 35.0%
GTTTGAAGACCAAATTACGT+TGG - tig0023536:56982-57001 Msa1409720:CDS 35.0%
AAGACCAAATTACGTTGGAA+AGG - tig0023536:56987-57006 Msa1409720:CDS 35.0%
! TAAGCTGCTGTTGTTGTTAT+AGG - tig0023536:57284-57303 Msa1409720:five_prime_UTR 35.0%
AAAGGAAAGAGGGAGGAAAT+GGG + tig0023536:53477-53496 None:intergenic 40.0%
GAAAAGATGAGTGGAAGGAA+AGG + tig0023536:53495-53514 None:intergenic 40.0%
GTCATGATAGAAGAGTAGAG+GGG + tig0023536:53656-53675 None:intergenic 40.0%
GGTCATGATAGAAGAGTAGA+GGG + tig0023536:53657-53676 None:intergenic 40.0%
AGGTCATGATAGAAGAGTAG+AGG + tig0023536:53658-53677 None:intergenic 40.0%
AGATAGAAGAAGAACCAACC+TGG + tig0023536:53695-53714 None:intergenic 40.0%
! GCTTTTACGTTTATTGCAGG+TGG - tig0023536:53782-53801 Msa1409720:intron 40.0%
ACGGAGATATTTCGGAAAGT+GGG + tig0023536:53862-53881 None:intergenic 40.0%
! TCCGTTTTCCTGAGTTTGAA+AGG + tig0023536:53922-53941 None:intergenic 40.0%
GAAGTACGTGAAGCAAGTAA+CGG - tig0023536:53961-53980 Msa1409720:CDS 40.0%
AAGTACGTGAAGCAAGTAAC+GGG - tig0023536:53962-53981 Msa1409720:CDS 40.0%
!! CAGATAATGGGTACTTTGAG+TGG - tig0023536:54070-54089 Msa1409720:CDS 40.0%
GGGTCATCTTGATGTTGTTA+AGG - tig0023536:54198-54217 Msa1409720:CDS 40.0%
TGACTAGCAGCAATATGCAA+AGG + tig0023536:54283-54302 None:intergenic 40.0%
TTGCATATTGCTGCTAGTCA+AGG - tig0023536:54283-54302 Msa1409720:CDS 40.0%
GCTACTCAAGGTCTAACAAT+TGG + tig0023536:54966-54985 None:intergenic 40.0%
CCACAAATTGAAGCTACTCA+AGG + tig0023536:54978-54997 None:intergenic 40.0%
CCTTGAGTAGCTTCAATTTG+TGG - tig0023536:54975-54994 Msa1409720:intron 40.0%
TTCATTTGCAGCCATTGTTC+AGG - tig0023536:55063-55082 Msa1409720:intron 40.0%
GGTGAATGAACTTCTGTCAA+AGG - tig0023536:55180-55199 Msa1409720:intron 40.0%
AGGCTTTCACGATTTCTGTA+TGG + tig0023536:55273-55292 None:intergenic 40.0%
GCATTGCATATGGCTGTAAA+AGG - tig0023536:55346-55365 Msa1409720:intron 40.0%
CATTGTTAATTCCTTCCCTG+TGG + tig0023536:56438-56457 None:intergenic 40.0%
AGGTGATTAACAAGCTCATG+TGG - tig0023536:56689-56708 Msa1409720:CDS 40.0%
GATTAACAAGCTCATGTGGT+TGG - tig0023536:56693-56712 Msa1409720:CDS 40.0%
! GCAATTCTGGTTACAGTTGT+TGG - tig0023536:56787-56806 Msa1409720:CDS 40.0%
! ATTCTGGTTACAGTTGTTGG+AGG - tig0023536:56790-56809 Msa1409720:CDS 40.0%
GTTGGAGGAGTGATAATATC+CGG - tig0023536:56805-56824 Msa1409720:CDS 40.0%
AGTCATAGTGCCAATAACTC+CGG + tig0023536:56827-56846 None:intergenic 40.0%
CTAAGAGAACAAGATCCATG+AGG - tig0023536:56863-56882 Msa1409720:CDS 40.0%
AATTCGATCAACCTCGGAAT+TGG + tig0023536:56950-56969 None:intergenic 40.0%
CGCATAAATTCGATCAACCT+CGG + tig0023536:56956-56975 None:intergenic 40.0%
AAAGTCGTGAGAAAGAGGGA+GGG + tig0023536:53454-53473 None:intergenic 45.0%
TGGGAAAGTCGTGAGAAAGA+GGG + tig0023536:53458-53477 None:intergenic 45.0%
ATGGGAAAGTCGTGAGAAAG+AGG + tig0023536:53459-53478 None:intergenic 45.0%
GAAAGGAAAGAGGGAGGAAA+TGG + tig0023536:53478-53497 None:intergenic 45.0%
GAGTGGAAGGAAAGGAAAGA+GGG + tig0023536:53487-53506 None:intergenic 45.0%
TGAGTGGAAGGAAAGGAAAG+AGG + tig0023536:53488-53507 None:intergenic 45.0%
GGAAGGAAGGATGAAAGTGA+AGG + tig0023536:53572-53591 None:intergenic 45.0%
ACCTCCAATTTCAACCTTCC+AGG - tig0023536:53674-53693 Msa1409720:three_prime_UTR 45.0%
ACCTGGAAGGTTGAAATTGG+AGG + tig0023536:53678-53697 None:intergenic 45.0%
CCAACCTGGAAGGTTGAAAT+TGG + tig0023536:53681-53700 None:intergenic 45.0%
CCAATTTCAACCTTCCAGGT+TGG - tig0023536:53678-53697 Msa1409720:three_prime_UTR 45.0%
AGAAGAAGAACCAACCTGGA+AGG + tig0023536:53691-53710 None:intergenic 45.0%
GACGGAGATATTTCGGAAAG+TGG + tig0023536:53863-53882 None:intergenic 45.0%
AGATGGCGACGGAGATATTT+CGG + tig0023536:53870-53889 None:intergenic 45.0%
GAGTTTGAAAGGACTAGAGC+CGG + tig0023536:53911-53930 None:intergenic 45.0%
GCTCTAGTCCTTTCAAACTC+AGG - tig0023536:53911-53930 Msa1409720:three_prime_UTR 45.0%
GAAAACGGATCGATCAAGCA+GGG - tig0023536:53933-53952 Msa1409720:exon 45.0%
CTTCATCTAGCAGCTCAAAG+AGG - tig0023536:54004-54023 Msa1409720:CDS 45.0%
TGTTATAAGTGGAGTGGCGT+TGG + tig0023536:55134-55153 None:intergenic 45.0%
TTATAACAGCAGCGACGAGA+GGG - tig0023536:55146-55165 Msa1409720:intron 45.0%
TTTGCATTTAGCTGCTCGTC+CGG - tig0023536:55246-55265 Msa1409720:intron 45.0%
TTCACGATTTCTGTATGGCC+CGG + tig0023536:55268-55287 None:intergenic 45.0%
AGGGCAAACTGCATTGCATA+TGG - tig0023536:55336-55355 Msa1409720:intron 45.0%
GCTGTGTTGCCAAATTTGTC+AGG + tig0023536:55430-55449 None:intergenic 45.0%
AACCAGGAAAAAGCGAGTAG+AGG - tig0023536:55462-55481 Msa1409720:CDS 45.0%
!!! TACCTCTACTCGCTTTTTCC+TGG + tig0023536:55467-55486 None:intergenic 45.0%
CATTTACGTTTGTGTCTGGC+AGG + tig0023536:56171-56190 None:intergenic 45.0%
!! CAATGTCAAGGGCTGTTTTG+TGG + tig0023536:56207-56226 None:intergenic 45.0%
TCTGATGACTCTTCAGAGAG+AGG + tig0023536:56241-56260 None:intergenic 45.0%
AAGGACATCCTCTCTCGATA+TGG - tig0023536:56265-56284 Msa1409720:intron 45.0%
AGGACATCCTCTCTCGATAT+GGG - tig0023536:56266-56285 Msa1409720:intron 45.0%
TAACGAGCTCAACCAACCAA+GGG - tig0023536:56300-56319 Msa1409720:intron 45.0%
AAGGGACGAACTGAGGAAAA+CGG - tig0023536:56318-56337 Msa1409720:intron 45.0%
GAAAGATGTTCACACACAGC+TGG - tig0023536:56354-56373 Msa1409720:intron 45.0%
CTATATTCACCGTACCTGGT+GGG - tig0023536:56512-56531 Msa1409720:intron 45.0%
TATATTCACCGTACCTGGTG+GGG - tig0023536:56513-56532 Msa1409720:intron 45.0%
!! TAACGATGATGGATCAGGAG+TGG - tig0023536:56537-56556 Msa1409720:intron 45.0%
AGCAGAGAAAAGGGTAGTTG+AGG - tig0023536:56669-56688 Msa1409720:CDS 45.0%
CTGCTTCTCTTTCTTCCTCA+TGG + tig0023536:56881-56900 None:intergenic 45.0%
CTCTGAATGATGCCATGAGT+TGG + tig0023536:56923-56942 None:intergenic 45.0%
TTCAGAGTTCTCCAATTCCG+AGG - tig0023536:56936-56955 Msa1409720:CDS 45.0%
!! GAGTTTTGTGCACTTCAGCA+TGG - tig0023536:57352-57371 Msa1409720:five_prime_UTR 45.0%
!!! TTTTTTTTTTTTTTTTTGAA+AGG + tig0023536:54681-54700 None:intergenic 5.0%
AAGTCGTGAGAAAGAGGGAG+GGG + tig0023536:53453-53472 None:intergenic 50.0%
GAAAGTCGTGAGAAAGAGGG+AGG + tig0023536:53455-53474 None:intergenic 50.0%
TGGAAGGAAAGGAAAGAGGG+AGG + tig0023536:53484-53503 None:intergenic 50.0%
! TCGGAAAGTGGGTTCTGTGT+TGG + tig0023536:53851-53870 None:intergenic 50.0%
GGAAAACGGATCGATCAAGC+AGG - tig0023536:53932-53951 Msa1409720:exon 50.0%
GCAGCTCAAAGAGGTGATGT+TGG - tig0023536:54013-54032 Msa1409720:CDS 50.0%
GCTGCTAGTCAAGGACATCA+TGG - tig0023536:54292-54311 Msa1409720:CDS 50.0%
! ATAAGTGGAGTGGCGTTGGA+TGG + tig0023536:55130-55149 None:intergenic 50.0%
CGCTGCTGTTATAAGTGGAG+TGG + tig0023536:55140-55159 None:intergenic 50.0%
CTCGTCGCTGCTGTTATAAG+TGG + tig0023536:55145-55164 None:intergenic 50.0%
CTTATAACAGCAGCGACGAG+AGG - tig0023536:55145-55164 Msa1409720:intron 50.0%
GTCAAAGGATGGCAGCTTGT+TGG - tig0023536:55195-55214 Msa1409720:intron 50.0%
TTGCATTTAGCTGCTCGTCC+GGG - tig0023536:55247-55266 Msa1409720:intron 50.0%
TTGAGGGTCTTTGCTGAGCA+AGG + tig0023536:55293-55312 None:intergenic 50.0%
GCTCAGCAAAGACCCTCAAT+TGG - tig0023536:55294-55313 Msa1409720:intron 50.0%
GCAAAGACCCTCAATTGGCT+CGG - tig0023536:55299-55318 Msa1409720:intron 50.0%
CTCGGAGGACTGACAAGAAA+GGG - tig0023536:55317-55336 Msa1409720:intron 50.0%
TGGCAACACAGCATTGCATG+TGG - tig0023536:55438-55457 Msa1409720:intron 50.0%
GGACATCCTCTCTCGATATG+GGG - tig0023536:56267-56286 Msa1409720:intron 50.0%
TAAGTGCCCCATATCGAGAG+AGG + tig0023536:56276-56295 None:intergenic 50.0%
CTAACGAGCTCAACCAACCA+AGG - tig0023536:56299-56318 Msa1409720:intron 50.0%
! GTTTTCCTCAGTTCGTCCCT+TGG + tig0023536:56319-56338 None:intergenic 50.0%
GTGGAGTTTCCTCAGCTCTT+TGG + tig0023536:56419-56438 None:intergenic 50.0%
AAGAGCTGAGGAAACTCCAC+AGG - tig0023536:56419-56438 Msa1409720:intron 50.0%
AGAGCTGAGGAAACTCCACA+GGG - tig0023536:56420-56439 Msa1409720:intron 50.0%
GCTGCTATATTCACCGTACC+TGG - tig0023536:56508-56527 Msa1409720:intron 50.0%
GCTATATTCACCGTACCTGG+TGG - tig0023536:56511-56530 Msa1409720:intron 50.0%
ATCGTTATCCCCACCAGGTA+CGG + tig0023536:56524-56543 None:intergenic 50.0%
!! GGGGATAACGATGATGGATC+AGG - tig0023536:56532-56551 Msa1409720:intron 50.0%
! GTTGGCTTCTGTCTGTACCT+CGG - tig0023536:56711-56730 Msa1409720:CDS 50.0%
AGAGGCAATAAATGCCACCG+AGG + tig0023536:56731-56750 None:intergenic 50.0%
GCCTCTTCGTACATAGTCGT+CGG - tig0023536:56745-56764 Msa1409720:CDS 50.0%
ACCGACGACTATGTACGAAG+AGG + tig0023536:56749-56768 None:intergenic 50.0%
CTTCGTACATAGTCGTCGGT+CGG - tig0023536:56749-56768 Msa1409720:CDS 50.0%
AGAAAGAGAAGCAGCTAGCC+AGG - tig0023536:56887-56906 Msa1409720:CDS 50.0%
! TGAGTTGGATCCACTTCTCC+TGG + tig0023536:56908-56927 None:intergenic 50.0%
GGAGAAGTGGATCCAACTCA+TGG - tig0023536:56908-56927 Msa1409720:CDS 50.0%
!! AAGACCCTCAATTGGCTCGG+AGG - tig0023536:55302-55321 Msa1409720:intron 55.0%
CAGTCCTCCGAGCCAATTGA+GGG + tig0023536:55309-55328 None:intergenic 55.0%
TCAGTCCTCCGAGCCAATTG+AGG + tig0023536:55310-55329 None:intergenic 55.0%
GCTCGGAGGACTGACAAGAA+AGG - tig0023536:55316-55335 Msa1409720:intron 55.0%
AAAAGGGCAGAGCTGTGACG+TGG - tig0023536:55363-55382 Msa1409720:intron 55.0%
CAGCATTGCATGTGGCAACC+AGG - tig0023536:55446-55465 Msa1409720:CDS 55.0%
ACCAACCAAGGGACGAACTG+AGG - tig0023536:56311-56330 Msa1409720:intron 55.0%
TCCTCAGTTCGTCCCTTGGT+TGG + tig0023536:56315-56334 None:intergenic 55.0%
CTGAGGAAACTCCACAGGGA+AGG - tig0023536:56424-56443 Msa1409720:intron 55.0%
! TGCCACAAACTCGGTGACAG+TGG - tig0023536:56456-56475 Msa1409720:intron 55.0%
CACCACTGTCACCGAGTTTG+TGG + tig0023536:56461-56480 None:intergenic 55.0%
! CACAAACTCGGTGACAGTGG+TGG - tig0023536:56459-56478 Msa1409720:intron 55.0%
CCATCATCGTTATCCCCACC+AGG + tig0023536:56529-56548 None:intergenic 55.0%
CCTGGTGGGGATAACGATGA+TGG - tig0023536:56526-56545 Msa1409720:intron 55.0%
!! GAACGAGTGGGCTGCAATTC+TGG - tig0023536:56774-56793 Msa1409720:CDS 55.0%
AAGCAGCTAGCCAGGAGAAG+TGG - tig0023536:56895-56914 Msa1409720:CDS 55.0%
GGCTTCTGTCTGTACCTCGG+TGG - tig0023536:56714-56733 Msa1409720:CDS 60.0%
CGTACATAGTCGTCGGTCGG+AGG - tig0023536:56752-56771 Msa1409720:CDS 60.0%
! GACGAGAGGGCACGTCGAAG+TGG - tig0023536:55159-55178 Msa1409720:intron 65.0%
TCGTCGGTCGGAGGAACGAG+TGG - tig0023536:56761-56780 Msa1409720:CDS 65.0%
CGTCGGTCGGAGGAACGAGT+GGG - tig0023536:56762-56781 Msa1409720:CDS 65.0%
GCGGACGACGGAGATGGCGA+CGG + tig0023536:53881-53900 None:intergenic 70.0%
GCCATCTCCGTCGTCCGCGA+CGG - tig0023536:53883-53902 Msa1409720:three_prime_UTR 70.0%
GCCGTCGCGGACGACGGAGA+TGG + tig0023536:53887-53906 None:intergenic 75.0%
GACTAGAGCCGGCGCCGTCG+CGG + tig0023536:53900-53919 None:intergenic 75.0%
! TCCGTCGTCCGCGACGGCGC+CGG - tig0023536:53889-53908 Msa1409720:three_prime_UTR 80.0%
! GCCGGCGCCGTCGCGGACGA+CGG + tig0023536:53893-53912 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
tig0023536 gene 53443 57480 53443 ID=Msa1409720;Name=Msa1409720
tig0023536 mRNA 53443 57480 53443 ID=Msa1409720-mRNA-1;Parent=Msa1409720;Name=Msa1409720-mRNA-1;_AED=0.06;_eAED=0.06;_QI=228|1|1|1|0.75|0.6|5|414|593
tig0023536 exon 53443 53720 53443 ID=Msa1409720-mRNA-1:exon:26167;Parent=Msa1409720-mRNA-1
tig0023536 exon 53806 54775 53806 ID=Msa1409720-mRNA-1:exon:26166;Parent=Msa1409720-mRNA-1
tig0023536 exon 55440 55849 55440 ID=Msa1409720-mRNA-1:exon:26165;Parent=Msa1409720-mRNA-1
tig0023536 exon 56610 57122 56610 ID=Msa1409720-mRNA-1:exon:26164;Parent=Msa1409720-mRNA-1
tig0023536 exon 57228 57480 57228 ID=Msa1409720-mRNA-1:exon:26163;Parent=Msa1409720-mRNA-1
tig0023536 five_prime_UTR 57253 57480 57253 ID=Msa1409720-mRNA-1:five_prime_utr;Parent=Msa1409720-mRNA-1
tig0023536 CDS 57228 57252 57228 ID=Msa1409720-mRNA-1:cds;Parent=Msa1409720-mRNA-1
tig0023536 CDS 56610 57122 56610 ID=Msa1409720-mRNA-1:cds;Parent=Msa1409720-mRNA-1
tig0023536 CDS 55440 55849 55440 ID=Msa1409720-mRNA-1:cds;Parent=Msa1409720-mRNA-1
tig0023536 CDS 53942 54775 53942 ID=Msa1409720-mRNA-1:cds;Parent=Msa1409720-mRNA-1
tig0023536 three_prime_UTR 53806 53941 53806 ID=Msa1409720-mRNA-1:three_prime_utr;Parent=Msa1409720-mRNA-1
tig0023536 three_prime_UTR 53443 53720 53443 ID=Msa1409720-mRNA-1:three_prime_utr;Parent=Msa1409720-mRNA-1
Gene Sequence

>Msa1409720

ATGACCTCCAATTTCAACCTTCCAGGTGGAGCTCAAACTCAAAGAGATTCAGAAAAAGGAACGATGATGACACCAACACAGAACCCACTTTCCGAAATATCTCCGTCGCCATCTCCGTCGTCCGCGACGGCGCCGGCTCTAGTCCTTTCAAACTCAGGAAAACGGATCGATCAAGCAGGGAAGAAGAAGTACGTGAAGCAAGTAACGGGTCGTCACAACGACACAGAGCTTCATCTAGCAGCTCAAAGAGGTGATGTTGGTGCTGTGAGACAGATCCTTCTTGATATTGATTCTCAGATAATGGGTACTTTGAGTGGTGATGATGATGTTGATCTTAACGCTGAGATTGCTGAAGTTCGTGCTCTTGTTGTGAATGAAGAGAATGAACTTGGTGAAACTGCTTTGTTTACTGCTGCTGAAAAGGGTCATCTTGATGTTGTTAAGGAGTTGTTGAAACATTCAACTCTTCAAACTGTTTCTAAAAAGAATCGATCTGGTTTTGATCCTTTGCATATTGCTGCTAGTCAAGGACATCATGCCATTGTTCAGGTTTTACTAGATTATGACCCAAGCTTAAGCAAAACTATTGGTCCATCCAACGCCACTCCACTTATAACAGCAGCGACGAGAGGGCACGTCGAAGTGGTGAATGAACTTCTGTCAAAGGATGGCAGCTTGTTGGAAATTGCAAGATCCAATGGAAAAAATGCTTTGCATTTAGCTGCTCGTCCGGGCCATACAGAAATCGTGAAAGCCTTGCTCAGCAAAGACCCTCAATTGGCTCGGAGGACTGACAAGAAAGGGCAAACTGCATTGCATATGGCTGTAAAAGGGCAGAGCTGTGACGTGGTGAAATTGCTTCTTGATGCAGATGCTGCTATTGTTATGCTTCCTGACAAATTTGGCAACACAGCATTGCATGTGGCAACCAGGAAAAAGCGAGTAGAGATAGTGAATGAGTTGTTACTCCTGCCAGACACAAACGTAAATGCATTAACACGAGACCACAAAACAGCCCTTGACATTGCTGAAAATCTTCCTCTCTCTGAAGAGTCATCAGATATAAAGGACATCCTCTCTCGATATGGGGCACTTAGAGCTAACGAGCTCAACCAACCAAGGGACGAACTGAGGAAAACGGTTACACAAATTAAGAAAGATGTTCACACACAGCTGGAACAAACCAAGAGAACCAACAAAAATGTCCACAATATATCCAAAGAGCTGAGGAAACTCCACAGGGAAGGAATTAACAATGCCACAAACTCGGTGACAGTGGTGGCTGTGTTGTTTGCTACAGTTGCTTTTGCTGCTATATTCACCGTACCTGGTGGGGATAACGATGATGGATCAGGAGTGGTAGCGAGTTATTCTGCTTTTAAAATTTTCTTCATATTCAATGCTATTGCGCTTTTTACATCTTTGGCTGTTGTGGTTGTTCAAATTACCCTTGTTAGAGGTGAAACTAAAGCAGAGAAAAGGGTAGTTGAGGTGATTAACAAGCTCATGTGGTTGGCTTCTGTCTGTACCTCGGTGGCATTTATTGCCTCTTCGTACATAGTCGTCGGTCGGAGGAACGAGTGGGCTGCAATTCTGGTTACAGTTGTTGGAGGAGTGATAATATCCGGAGTTATTGGCACTATGACTTACTATGTAGTGAGGTCTAAGAGAACAAGATCCATGAGGAAGAAAGAGAAGCAGCTAGCCAGGAGAAGTGGATCCAACTCATGGCATCATTCAGAGTTCTCCAATTCCGAGGTTGATCGAATTTATGCGCTCTAA
Protein sequence

>Msa1409720

MTSNFNLPGGAQTQRDSEKGTMMTPTQNPLSEISPSPSPSSATAPALVLSNSGKRIDQAGKKKYVKQVTGRHNDTELHLAAQRGDVGAVRQILLDIDSQIMGTLSGDDDVDLNAEIAEVRALVVNEENELGETALFTAAEKGHLDVVKELLKHSTLQTVSKKNRSGFDPLHIAASQGHHAIVQVLLDYDPSLSKTIGPSNATPLITAATRGHVEVVNELLSKDGSLLEIARSNGKNALHLAARPGHTEIVKALLSKDPQLARRTDKKGQTALHMAVKGQSCDVVKLLLDADAAIVMLPDKFGNTALHVATRKKRVEIVNELLLLPDTNVNALTRDHKTALDIAENLPLSEESSDIKDILSRYGALRANELNQPRDELRKTVTQIKKDVHTQLEQTKRTNKNVHNISKELRKLHREGINNATNSVTVVAVLFATVAFAAIFTVPGGDNDDGSGVVASYSAFKIFFIFNAIALFTSLAVVVVQITLVRGETKAEKRVVEVINKLMWLASVCTSVAFIASSYIVVGRRNEWAAILVTVVGGVIISGVIGTMTYYVVRSKRTRSMRKKEKQLARRSGSNSWHHSEFSNSEVDRIYAL*