AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0045780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0045780 MtrunA17_Chr1g0212971 99.157 593 5 0 1 593 1 593 0.0 1201
Msa0045780 MtrunA17_Chr7g0263471 73.042 549 141 4 46 593 40 582 0.0 791
Msa0045780 MtrunA17_Chr2g0327711 46.209 554 253 9 61 593 15 544 2.55e-147 436
Msa0045780 MtrunA17_Chr4g0030551 47.126 522 237 8 58 563 16 514 2.40e-145 431
Msa0045780 MtrunA17_Chr7g0276681 45.421 546 255 7 61 593 31 546 7.71e-137 409
Msa0045780 MtrunA17_Chr1g0161231 45.436 482 246 7 127 593 52 531 5.60e-128 386
Msa0045780 MtrunA17_Chr7g0263421 42.620 542 273 7 66 593 3 520 2.95e-125 379
Msa0045780 MtrunA17_Chr8g0391711 43.659 481 256 4 127 593 59 538 6.24e-125 378
Msa0045780 MtrunA17_Chr2g0331191 22.951 610 356 14 4 567 69 610 8.96e-27 115
Msa0045780 MtrunA17_Chr2g0331191 25.403 248 184 1 96 343 1 247 1.05e-20 96.3
Msa0045780 MtrunA17_Chr3g0136651 22.707 458 298 12 138 577 8 427 3.84e-25 108
Msa0045780 MtrunA17_Chr2g0331201 28.896 308 202 5 74 367 37 341 1.77e-21 99.0
Msa0045780 MtrunA17_Chr2g0331201 26.949 449 250 15 130 522 137 563 1.68e-20 95.9
Msa0045780 MtrunA17_Chr2g0331201 26.471 238 173 2 107 343 11 247 4.28e-19 91.3
Msa0045780 MtrunA17_Chr8g0361991 25.469 373 214 10 76 445 10 321 1.60e-19 91.7
Msa0045780 MtrunA17_Chr2g0328031 31.707 205 121 4 134 322 14 215 1.99e-19 91.3
Msa0045780 MtrunA17_Chr8g0370361 26.756 299 178 6 169 452 46 318 5.30e-19 90.1
Msa0045780 MtrunA17_Chr8g0367671 23.786 412 245 12 169 543 47 426 1.21e-18 89.0
Msa0045780 MtrunA17_Chr1g0178581 24.565 460 262 15 138 556 15 430 3.83e-18 87.4
Msa0045780 MtrunA17_Chr7g0233331 24.611 321 206 6 138 445 12 309 2.26e-16 81.6
Msa0045780 MtrunA17_Chr5g0439361 25.908 413 253 13 169 562 23 401 3.14e-16 81.3
Msa0045780 MtrunA17_Chr5g0439351 27.128 376 238 11 169 533 338 688 3.67e-16 82.0
Msa0045780 MtrunA17_Chr5g0439161 24.319 477 310 14 128 562 207 674 6.51e-16 81.3
Msa0045780 MtrunA17_Chr8g0369921 23.653 427 245 13 169 556 45 429 7.85e-16 80.1
Msa0045780 MtrunA17_Chr4g0031491 30.288 208 124 7 132 322 13 216 9.13e-16 80.1
Msa0045780 MtrunA17_Chr8g0368621 24.315 292 180 6 169 445 42 307 2.74e-15 77.4
Msa0045780 MtrunA17_Chr3g0100301 24.552 391 245 14 189 542 15 392 2.94e-15 78.2
Msa0045780 MtrunA17_Chr7g0262731 27.917 240 158 6 135 361 9 246 3.95e-15 78.6
Msa0045780 MtrunA17_Chr7g0221531 23.099 355 208 9 169 488 44 368 1.99e-14 75.5
Msa0045780 MtrunA17_Chr8g0354071 23.684 342 198 10 177 480 2 318 2.79e-14 75.1
Msa0045780 MtrunA17_Chr8g0364031 25.000 336 200 10 138 453 13 316 5.79e-14 74.3
Msa0045780 MtrunA17_Chr5g0418551 33.708 178 111 5 132 306 20 193 1.02e-13 71.2
Msa0045780 MtrunA17_Chr7g0263431 71.429 49 13 1 274 321 1 49 1.26e-13 65.5
Msa0045780 MtrunA17_Chr3g0093531 23.684 342 198 10 177 480 2 318 1.90e-13 72.4
Msa0045780 MtrunA17_Chr3g0083331 24.917 301 178 9 169 445 45 321 2.71e-13 72.4
Msa0045780 MtrunA17_Chr8g0353311 21.809 376 234 9 169 509 49 399 4.61e-13 71.6
Msa0045780 MtrunA17_Chr8g0376971 26.183 317 194 9 72 352 77 389 6.24e-13 71.6
Msa0045780 MtrunA17_Chr1g0211241 29.952 207 137 5 120 322 120 322 1.10e-12 70.9
Msa0045780 MtrunA17_Chr3g0122941 28.342 187 125 3 131 309 105 290 1.56e-12 70.5
Msa0045780 MtrunA17_Chr3g0122941 27.208 283 173 9 100 355 108 384 2.92e-12 69.7
Msa0045780 MtrunA17_Chr6g0461061 27.935 247 158 7 90 323 125 364 1.63e-11 67.0
Msa0045780 MtrunA17_Chr5g0410201 25.284 352 185 11 213 544 7 300 1.71e-11 65.9
Msa0045780 MtrunA17_Chr4g0064931 30.319 188 127 4 135 322 40 223 2.05e-11 67.0
Msa0045780 MtrunA17_Chr7g0262761 27.922 154 100 3 169 312 47 199 2.36e-11 63.5
Msa0045780 MtrunA17_Chr4g0073161 30.392 204 98 6 128 294 9 205 3.05e-11 66.2
Msa0045780 MtrunA17_Chr1g0196261 28.947 228 134 8 114 317 198 421 5.70e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0045780 AT3G12360.1 80.684 585 104 4 14 593 10 590 0.0 954
Msa0045780 AT3G12360.2 80.895 581 102 4 18 593 2 578 0.0 950
Msa0045780 AT3G09550.1 71.502 586 148 3 24 593 25 607 0.0 833
Msa0045780 AT3G09550.3 71.502 586 148 3 24 593 25 607 0.0 833
Msa0045780 AT3G09550.2 71.502 586 148 3 24 593 32 614 0.0 832
Msa0045780 AT2G31820.1 43.534 549 271 7 60 593 138 662 8.01e-138 416
Msa0045780 AT1G05640.1 44.404 554 270 7 56 593 96 627 7.62e-136 410
Msa0045780 AT5G60070.1 46.595 558 244 13 61 593 20 548 3.40e-135 405
Msa0045780 AT2G01680.1 43.110 508 273 8 99 593 28 532 2.25e-129 390
Msa0045780 AT1G07710.2 44.565 552 264 5 61 593 15 543 2.44e-129 390
Msa0045780 AT1G07710.1 44.565 552 264 5 61 593 15 543 2.44e-129 390
Msa0045780 AT5G02620.4 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa0045780 AT5G02620.1 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa0045780 AT5G02620.3 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa0045780 AT5G02620.5 41.899 537 248 8 61 556 5 518 1.15e-118 363
Msa0045780 AT5G02620.2 41.899 537 248 8 61 556 5 518 1.15e-118 363
Msa0045780 AT1G10340.1 25.202 496 309 12 111 552 51 538 2.25e-34 138
Msa0045780 AT5G54710.1 27.308 520 284 17 68 552 97 557 1.49e-33 135
Msa0045780 AT5G54710.2 27.308 520 284 17 68 552 142 602 2.33e-33 135
Msa0045780 AT1G10340.2 24.593 492 313 11 111 552 51 534 2.38e-33 135
Msa0045780 AT1G34050.1 26.145 524 294 15 108 554 28 535 6.31e-33 134
Msa0045780 AT2G24600.3 26.892 502 290 18 72 538 70 529 3.55e-32 131
Msa0045780 AT2G24600.4 26.892 502 290 18 72 538 70 529 3.55e-32 131
Msa0045780 AT4G03500.2 26.047 549 280 17 58 522 54 560 2.29e-30 126
Msa0045780 AT4G03500.3 26.047 549 280 17 58 522 54 560 2.29e-30 126
Msa0045780 AT4G03500.1 26.047 549 280 17 58 522 77 583 2.43e-30 126
Msa0045780 AT1G03670.1 25.581 516 328 9 74 562 107 593 2.56e-26 114
Msa0045780 AT4G03490.3 27.674 430 267 12 133 551 180 576 5.97e-25 110
Msa0045780 AT4G03490.5 27.830 424 262 12 133 545 170 560 7.94e-25 109
Msa0045780 AT4G03490.4 27.830 424 262 12 133 545 180 570 8.14e-25 109
Msa0045780 AT4G03460.2 26.406 409 256 8 128 522 158 535 8.35e-24 106
Msa0045780 AT4G03460.1 26.406 409 256 8 128 522 190 567 9.03e-24 106
Msa0045780 AT4G03490.2 24.915 586 332 18 65 574 9 562 1.03e-23 106
Msa0045780 AT5G50140.1 22.913 515 297 16 126 563 22 513 1.05e-23 105
Msa0045780 AT4G03490.1 25.277 542 318 15 65 551 19 528 2.42e-23 105
Msa0045780 AT5G54700.2 28.435 313 159 9 223 523 20 279 1.39e-22 99.8
Msa0045780 AT4G10720.1 24.096 415 258 12 169 559 40 421 3.32e-22 100
Msa0045780 AT5G54700.1 27.809 356 182 12 202 523 2 316 6.75e-22 99.8
Msa0045780 AT4G10720.2 25.076 327 210 6 169 477 40 349 1.96e-21 97.4
Msa0045780 AT4G14390.2 24.722 449 281 11 128 556 226 637 2.16e-20 95.9
Msa0045780 AT4G14390.4 24.722 449 281 11 128 556 245 656 2.63e-20 95.5
Msa0045780 AT4G14390.1 24.889 450 280 12 128 556 245 657 2.98e-20 95.5
Msa0045780 AT4G03480.1 26.076 395 232 9 118 482 295 659 1.57e-19 93.2
Msa0045780 AT4G03480.2 26.076 395 232 9 118 482 292 656 1.65e-19 93.2
Msa0045780 AT4G05040.3 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0045780 AT4G05040.2 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0045780 AT4G05040.5 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0045780 AT4G05040.4 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0045780 AT4G05040.1 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0045780 AT4G05040.6 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0045780 AT4G05040.7 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa0045780 AT5G15500.2 23.427 461 288 15 130 559 1 427 2.70e-18 88.2
Msa0045780 AT2G24600.2 26.420 405 232 14 72 450 70 434 1.75e-17 86.3
Msa0045780 AT2G24600.1 26.420 405 232 14 72 450 70 434 1.75e-17 86.3
Msa0045780 AT4G03450.2 24.505 555 306 17 58 528 45 570 1.81e-17 86.7
Msa0045780 AT5G51160.1 25.000 352 198 8 157 478 38 353 2.38e-17 85.5
Msa0045780 AT4G03450.1 24.505 555 306 17 58 528 45 570 5.28e-17 85.1
Msa0045780 AT4G03470.1 26.044 503 294 18 77 542 157 618 1.34e-16 84.0
Msa0045780 AT3G04140.1 27.622 286 160 8 100 342 105 386 2.92e-16 82.8
Msa0045780 AT1G14500.1 22.769 325 215 5 169 475 40 346 3.84e-15 78.6
Msa0045780 AT2G03430.1 34.659 176 106 5 135 306 16 186 8.50e-15 74.7
Msa0045780 AT4G14390.3 26.748 329 195 8 128 443 226 521 4.92e-13 72.4
Msa0045780 AT5G54620.1 29.570 186 119 2 169 342 40 225 8.08e-13 71.2
Msa0045780 AT5G54610.1 27.717 184 123 2 169 342 40 223 8.13e-13 71.2
Msa0045780 AT3G18670.1 23.111 450 296 12 123 530 113 554 1.09e-12 71.2
Msa0045780 AT2G28840.1 27.273 209 129 7 132 322 12 215 2.01e-12 70.1
Msa0045780 AT4G03440.1 23.126 467 308 12 104 550 258 693 3.95e-12 69.7
Msa0045780 AT4G03440.2 23.126 467 308 12 104 550 251 686 4.36e-12 69.7
Msa0045780 AT4G03440.3 23.126 467 308 12 104 550 251 686 4.36e-12 69.7
Msa0045780 AT4G14400.2 23.242 512 310 20 75 538 71 547 4.01e-11 66.2
Msa0045780 AT4G14400.3 23.242 512 310 20 75 538 71 547 4.01e-11 66.2
Msa0045780 AT3G18670.2 23.684 304 183 7 236 530 346 609 4.70e-11 66.2
Msa0045780 AT4G14400.1 23.242 512 310 20 75 538 137 613 5.68e-11 65.9

Find 172 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTGCATATGGCTGTAAA+AGG 0.222840 1_1:+77736313 Msa0045780:CDS
TTATCCTTTCCAACGTAATT+TGG 0.223179 1_1:-77737958 None:intergenic
TGATATTGATTCTCAGATAA+TGG 0.227356 1_1:+77735060 Msa0045780:CDS
TAAGCTGCTGTTGTTGTTAT+AGG 0.245673 1_1:+77738166 Msa0045780:three_prime_UTR
AATCATCATAATAATAATTT+AGG 0.249295 1_1:-77734591 None:intergenic
GTGGAGTTTCCTCAGCTCTT+TGG 0.250605 1_1:-77737383 None:intergenic
GTTATGCTTCCTGACAAATT+TGG 0.259995 1_1:+77736385 Msa0045780:CDS
GAAAGGAAAGAGGGAGGAAA+TGG 0.261658 1_1:-77734473 None:intergenic
GAACGAGTGGGCTGCAATTC+TGG 0.267500 1_1:+77737741 Msa0045780:CDS
ACCTCCAATTTCAACCTTCC+AGG 0.283929 1_1:+77734678 Msa0045780:CDS
ACTCAAAGAGATTCAGAAAA+AGG 0.285730 1_1:+77734815 Msa0045780:CDS
AAGGACATCCTCTCTCGATA+TGG 0.290107 1_1:+77737232 Msa0045780:CDS
CCAACCTGGAAGGTTGAAAT+TGG 0.303178 1_1:-77734682 None:intergenic
GTTATAGGATCATAACAAAT+TGG 0.307029 1_1:+77738181 Msa0045780:three_prime_UTR
TTCATTTGCAGCCATTGTTC+AGG 0.330505 1_1:+77736030 Msa0045780:intron
CTGCTTCTCTTTCTTCCTCA+TGG 0.331730 1_1:-77737845 None:intergenic
GCAATTCTGGTTACAGTTGT+TGG 0.335512 1_1:+77737754 Msa0045780:CDS
GATATTGATTCTCAGATAAT+GGG 0.350929 1_1:+77735061 Msa0045780:CDS
AATTCGATCAACCTCGGAAT+TGG 0.351942 1_1:-77737914 None:intergenic
AAAGGAAAGAGGGAGGAAAT+GGG 0.356439 1_1:-77734472 None:intergenic
TGAAGAGTCATCAGATATAA+AGG 0.366092 1_1:+77737213 Msa0045780:CDS
TAATAAGTGTTGGCTTTGAT+TGG 0.372963 1_1:-77734532 None:intergenic
TTTGTTTACTGCTGCTGAAA+AGG 0.377535 1_1:+77735180 Msa0045780:CDS
AGATGGCGACGGAGATATTT+CGG 0.380641 1_1:-77734870 None:intergenic
GTTCAAATTACCCTTGTTAG+AGG 0.385400 1_1:+77737604 Msa0045780:CDS
GTGATAATATCCGGAGTTAT+TGG 0.388823 1_1:+77737781 Msa0045780:CDS
GTGAAACTAAAGCAGAGAAA+AGG 0.392404 1_1:+77737626 Msa0045780:CDS
TTTGCATTTAGCTGCTCGTC+CGG 0.403120 1_1:+77736213 Msa0045780:CDS
GTCAAAGGATGGCAGCTTGT+TGG 0.404723 1_1:+77736162 Msa0045780:CDS
GGGTCATCTTGATGTTGTTA+AGG 0.408314 1_1:+77735201 Msa0045780:CDS
AGAAAGAGAAGCAGCTAGCC+AGG 0.411228 1_1:+77737854 Msa0045780:CDS
AGGACATCCTCTCTCGATAT+GGG 0.415929 1_1:+77737233 Msa0045780:CDS
CACCACTGTCACCGAGTTTG+TGG 0.435710 1_1:-77737425 None:intergenic
CTCGTCGCTGCTGTTATAAG+TGG 0.455896 1_1:-77736109 None:intergenic
TCCGTCGTCCGCGACGGCGC+CGG 0.456322 1_1:+77734892 Msa0045780:CDS
ATGGGAAAGTCGTGAGAAAG+AGG 0.462631 1_1:-77734454 None:intergenic
AGGCTTTCACGATTTCTGTA+TGG 0.466846 1_1:-77736237 None:intergenic
TTGCATTTAGCTGCTCGTCC+GGG 0.469773 1_1:+77736214 Msa0045780:CDS
GCTGCTATATTCACCGTACC+TGG 0.472210 1_1:+77737475 Msa0045780:CDS
TCCTCAGTTCGTCCCTTGGT+TGG 0.473921 1_1:-77737279 None:intergenic
GAGTGGAAGGAAAGGAAAGA+GGG 0.474346 1_1:-77734482 None:intergenic
CATTTACGTTTGTGTCTGGC+AGG 0.475438 1_1:-77737135 None:intergenic
TTGTTTACTGCTGCTGAAAA+GGG 0.479987 1_1:+77735181 Msa0045780:CDS
GCTCAGCAAAGACCCTCAAT+TGG 0.481998 1_1:+77736261 Msa0045780:CDS
CTTTAGTTTCACCTCTAACA+AGG 0.482809 1_1:-77737615 None:intergenic
CCAAGCTTAAGCAAAACTAT+TGG 0.486154 1_1:+77736070 Msa0045780:CDS
GAAAAGATGAGTGGAAGGAA+AGG 0.490183 1_1:-77734490 None:intergenic
TGAGTTGGATCCACTTCTCC+TGG 0.491866 1_1:-77737872 None:intergenic
GGGGATAACGATGATGGATC+AGG 0.492044 1_1:+77737499 Msa0045780:CDS
CAGTCCTCCGAGCCAATTGA+GGG 0.492228 1_1:-77736273 None:intergenic
TAATAATAATTTAGGAAGGA+AGG 0.493048 1_1:-77734583 None:intergenic
TCCTTTCAAACTCAGGAAAA+CGG 0.494021 1_1:+77734921 Msa0045780:CDS
TGAGTGGAAGGAAAGGAAAG+AGG 0.495388 1_1:-77734483 None:intergenic
TGAAACTAAAGCAGAGAAAA+GGG 0.501105 1_1:+77737627 Msa0045780:CDS
AGGGCAAACTGCATTGCATA+TGG 0.501695 1_1:+77736303 Msa0045780:CDS
TCAGCTCTTTGGATATATTG+TGG 0.502735 1_1:-77737372 None:intergenic
GGTCATGATAGAAGAGTAGA+GGG 0.503098 1_1:-77734658 None:intergenic
ATCGTTATCCCCACCAGGTA+CGG 0.503271 1_1:-77737488 None:intergenic
TTGAGGGTCTTTGCTGAGCA+AGG 0.503619 1_1:-77736257 None:intergenic
AATGCATTTACGTTTGTGTC+TGG 0.503703 1_1:-77737139 None:intergenic
TTGGAAATTGCAAGATCCAA+TGG 0.508338 1_1:+77736181 Msa0045780:CDS
AAGTACGTGAAGCAAGTAAC+GGG 0.508570 1_1:+77734965 Msa0045780:CDS
GCTCGGAGGACTGACAAGAA+AGG 0.509842 1_1:+77736283 Msa0045780:CDS
AAAGAGAAAAGATGAGTGGA+AGG 0.510130 1_1:-77734495 None:intergenic
GACGGAGATATTTCGGAAAG+TGG 0.514348 1_1:-77734863 None:intergenic
CTCGGAGGACTGACAAGAAA+GGG 0.519611 1_1:+77736284 Msa0045780:CDS
TTCACGATTTCTGTATGGCC+CGG 0.525638 1_1:-77736232 None:intergenic
TATGCATTTGTATAAATCAC+AGG 0.525663 1_1:+77738263 Msa0045780:three_prime_UTR
GTTGGAGGAGTGATAATATC+CGG 0.527167 1_1:+77737772 Msa0045780:CDS
GCCGGCGCCGTCGCGGACGA+CGG 0.529146 1_1:-77734893 None:intergenic
CATTGCATATGGCTGTAAAA+GGG 0.531587 1_1:+77736314 Msa0045780:CDS
AAGACCAAATTACGTTGGAA+AGG 0.532286 1_1:+77737954 Msa0045780:three_prime_UTR
TGCTACAAGAAATGTCTTGG+AGG 0.533293 1_1:+77738397 Msa0045780:three_prime_UTR
GAAAGATGTTCACACACAGC+TGG 0.535788 1_1:+77737321 Msa0045780:CDS
CCTGGTGGGGATAACGATGA+TGG 0.535860 1_1:+77737493 Msa0045780:CDS
GCCATCTCCGTCGTCCGCGA+CGG 0.536247 1_1:+77734886 Msa0045780:CDS
GCTGTGTTGCCAAATTTGTC+AGG 0.537540 1_1:-77736394 None:intergenic
GGAAAACGGATCGATCAAGC+AGG 0.537963 1_1:+77734935 Msa0045780:CDS
CTATATTCACCGTACCTGGT+GGG 0.538227 1_1:+77737479 Msa0045780:CDS
CAGCATTGCATGTGGCAACC+AGG 0.541967 1_1:+77736413 Msa0045780:CDS
AAGCAGCTAGCCAGGAGAAG+TGG 0.542236 1_1:+77737862 Msa0045780:CDS
TCAGTCCTCCGAGCCAATTG+AGG 0.545108 1_1:-77736274 None:intergenic
TCGGAAAGTGGGTTCTGTGT+TGG 0.547229 1_1:-77734851 None:intergenic
GACTAGAGCCGGCGCCGTCG+CGG 0.548417 1_1:-77734900 None:intergenic
GAAAGTCGTGAGAAAGAGGG+AGG 0.548432 1_1:-77734450 None:intergenic
TTGCATATTGCTGCTAGTCA+AGG 0.549825 1_1:+77735286 Msa0045780:CDS
CAGATAATGGGTACTTTGAG+TGG 0.549949 1_1:+77735073 Msa0045780:CDS
TGGAAGGAAAGGAAAGAGGG+AGG 0.550810 1_1:-77734479 None:intergenic
AAGTCGTGAGAAAGAGGGAG+GGG 0.550978 1_1:-77734448 None:intergenic
TGGGAAAGTCGTGAGAAAGA+GGG 0.551251 1_1:-77734453 None:intergenic
CTCTGAATGATGCCATGAGT+TGG 0.551504 1_1:-77737887 None:intergenic
AAGAGCTGAGGAAACTCCAC+AGG 0.552962 1_1:+77737386 Msa0045780:CDS
ATCTATGTAAACTCTTGAAG+TGG 0.553547 1_1:-77738015 None:intergenic
ATCTATGTAAACTCTTGAAG+TGG 0.553547 1_1:-77738100 None:intergenic
ATTCTGGTTACAGTTGTTGG+AGG 0.554005 1_1:+77737757 Msa0045780:CDS
GATTAACAAGCTCATGTGGT+TGG 0.554304 1_1:+77737660 Msa0045780:CDS
AAGGGACGAACTGAGGAAAA+CGG 0.554349 1_1:+77737285 Msa0045780:CDS
AGGTCATGATAGAAGAGTAG+AGG 0.556929 1_1:-77734659 None:intergenic
AAAGTCGTGAGAAAGAGGGA+GGG 0.557510 1_1:-77734449 None:intergenic
ACCTGGAAGGTTGAAATTGG+AGG 0.558297 1_1:-77734679 None:intergenic
GCCGTCGCGGACGACGGAGA+TGG 0.559929 1_1:-77734887 None:intergenic
CCATCATCGTTATCCCCACC+AGG 0.564948 1_1:-77737493 None:intergenic
TTTAGTTTCACCTCTAACAA+GGG 0.565107 1_1:-77737614 None:intergenic
GTTTGAAGACCAAATTACGT+TGG 0.566835 1_1:+77737949 Msa0045780:three_prime_UTR
GCTACAAGAAATGTCTTGGA+GGG 0.566909 1_1:+77738398 Msa0045780:three_prime_UTR
GAGTTTGAAAGGACTAGAGC+CGG 0.567456 1_1:-77734911 None:intergenic
GCAAAGACCCTCAATTGGCT+CGG 0.569896 1_1:+77736266 Msa0045780:CDS
GCTCTAGTCCTTTCAAACTC+AGG 0.572097 1_1:+77734914 Msa0045780:CDS
CATTGTTAATTCCTTCCCTG+TGG 0.572226 1_1:-77737402 None:intergenic
AGCAGAGAAAAGGGTAGTTG+AGG 0.573019 1_1:+77737636 Msa0045780:CDS
TCGTCGGTCGGAGGAACGAG+TGG 0.574175 1_1:+77737728 Msa0045780:CDS
CGCTGCTGTTATAAGTGGAG+TGG 0.575162 1_1:-77736104 None:intergenic
GCTGCTAGTCAAGGACATCA+TGG 0.575482 1_1:+77735295 Msa0045780:CDS
ATAAGTGGAGTGGCGTTGGA+TGG 0.578501 1_1:-77736094 None:intergenic
GGAAGGAAGGATGAAAGTGA+AGG 0.582860 1_1:-77734570 None:intergenic
GCAGCTCAAAGAGGTGATGT+TGG 0.583391 1_1:+77735016 Msa0045780:CDS
GTGAATGAAGAGAATGAACT+TGG 0.587406 1_1:+77735148 Msa0045780:CDS
TCTGAGAATCAATATCAAGA+AGG 0.587872 1_1:-77735054 None:intergenic
ACGGAGATATTTCGGAAAGT+GGG 0.587917 1_1:-77734862 None:intergenic
TGTTATAAGTGGAGTGGCGT+TGG 0.592828 1_1:-77736098 None:intergenic
TTCAGAGTTCTCCAATTCCG+AGG 0.592884 1_1:+77737903 Msa0045780:CDS
GCATGAGTCATAATAAGTGT+TGG 0.594195 1_1:-77734542 None:intergenic
ATCATAATAATAATTTAGGA+AGG 0.594731 1_1:-77734587 None:intergenic
GCTATATTCACCGTACCTGG+TGG 0.598478 1_1:+77737478 Msa0045780:CDS
ATCTAGTAAAACCTGAACAA+TGG 0.600510 1_1:-77736041 None:intergenic
GCCTCTTCGTACATAGTCGT+CGG 0.600824 1_1:+77737712 Msa0045780:CDS
CGCATAAATTCGATCAACCT+CGG 0.601587 1_1:-77737920 None:intergenic
TGATAAAGAGAAAAGATGAG+TGG 0.602428 1_1:-77734499 None:intergenic
CGTACATAGTCGTCGGTCGG+AGG 0.602499 1_1:+77737719 Msa0045780:CDS
GTTGGCTTCTGTCTGTACCT+CGG 0.602568 1_1:+77737678 Msa0045780:CDS
CTGAGGAAACTCCACAGGGA+AGG 0.603249 1_1:+77737391 Msa0045780:CDS
TATATTCACCGTACCTGGTG+GGG 0.603908 1_1:+77737480 Msa0045780:CDS
GAAGTACGTGAAGCAAGTAA+CGG 0.604371 1_1:+77734964 Msa0045780:CDS
GGAGAAGTGGATCCAACTCA+TGG 0.605497 1_1:+77737875 Msa0045780:CDS
AGAAGAAGAACCAACCTGGA+AGG 0.609441 1_1:-77734692 None:intergenic
GACGAGAGGGCACGTCGAAG+TGG 0.609993 1_1:+77736126 Msa0045780:CDS
GGTGAATGAACTTCTGTCAA+AGG 0.612992 1_1:+77736147 Msa0045780:CDS
TTATAACAGCAGCGACGAGA+GGG 0.613377 1_1:+77736113 Msa0045780:CDS
AATGAACTTCTGTCAAAGGA+TGG 0.613527 1_1:+77736151 Msa0045780:CDS
TCTGATGACTCTTCAGAGAG+AGG 0.615975 1_1:-77737205 None:intergenic
AATTAACAATGCCACAAACT+CGG 0.616650 1_1:+77737414 Msa0045780:CDS
CGTCGGTCGGAGGAACGAGT+GGG 0.619749 1_1:+77737729 Msa0045780:CDS
ACCAACCAAGGGACGAACTG+AGG 0.620407 1_1:+77737278 Msa0045780:CDS
CTAACGAGCTCAACCAACCA+AGG 0.621035 1_1:+77737266 Msa0045780:CDS
CTTCATCTAGCAGCTCAAAG+AGG 0.623399 1_1:+77735007 Msa0045780:CDS
CTTCGTACATAGTCGTCGGT+CGG 0.624785 1_1:+77737716 Msa0045780:CDS
CTTATAACAGCAGCGACGAG+AGG 0.631608 1_1:+77736112 Msa0045780:CDS
TGCCACAAACTCGGTGACAG+TGG 0.632510 1_1:+77737423 Msa0045780:CDS
AGTCATAGTGCCAATAACTC+CGG 0.636725 1_1:-77737791 None:intergenic
TGGCAACACAGCATTGCATG+TGG 0.637663 1_1:+77736405 Msa0045780:CDS
GGACATCCTCTCTCGATATG+GGG 0.638757 1_1:+77737234 Msa0045780:CDS
TGACTAGCAGCAATATGCAA+AGG 0.639675 1_1:-77735283 None:intergenic
ACAATATATCCAAAGAGCTG+AGG 0.644642 1_1:+77737374 Msa0045780:CDS
AAAATTCTCAGTAACAGACT+AGG 0.646190 1_1:-77738375 None:intergenic
AAGACCCTCAATTGGCTCGG+AGG 0.651684 1_1:+77736269 Msa0045780:CDS
GCGGACGACGGAGATGGCGA+CGG 0.652416 1_1:-77734881 None:intergenic
GGCTTCTGTCTGTACCTCGG+TGG 0.655940 1_1:+77737681 Msa0045780:CDS
GAAAACGGATCGATCAAGCA+GGG 0.663000 1_1:+77734936 Msa0045780:CDS
TAACGATGATGGATCAGGAG+TGG 0.663853 1_1:+77737504 Msa0045780:CDS
TAAGTGCCCCATATCGAGAG+AGG 0.665007 1_1:-77737240 None:intergenic
AGGTGATTAACAAGCTCATG+TGG 0.671742 1_1:+77737656 Msa0045780:CDS
ACCGACGACTATGTACGAAG+AGG 0.681066 1_1:-77737713 None:intergenic
CACAAACTCGGTGACAGTGG+TGG 0.691648 1_1:+77737426 Msa0045780:CDS
TAACGAGCTCAACCAACCAA+GGG 0.693605 1_1:+77737267 Msa0045780:CDS
ACTAGGATAACATATAGACC+AGG 0.706188 1_1:-77738358 None:intergenic
CTATGACTTACTATGTAGTG+AGG 0.708286 1_1:+77737806 Msa0045780:CDS
CTAAGAGAACAAGATCCATG+AGG 0.720939 1_1:+77737830 Msa0045780:CDS
AGAGCTGAGGAAACTCCACA+GGG 0.730372 1_1:+77737387 Msa0045780:CDS
AAAAGGGCAGAGCTGTGACG+TGG 0.742175 1_1:+77736330 Msa0045780:CDS
GTCATGATAGAAGAGTAGAG+GGG 0.743790 1_1:-77734657 None:intergenic
AGAGGCAATAAATGCCACCG+AGG 0.784742 1_1:-77737695 None:intergenic
GATTTGTGAGAAGAATAACA+CGG 0.801731 1_1:+77738290 Msa0045780:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATCATAATAATAATTT+AGG - chr1_1:77734594-77734613 None:intergenic 10.0%
!!! GTTTTGTATTTTTTTTTTTT+TGG + chr1_1:77735626-77735645 Msa0045780:intron 10.0%
!! ATCATAATAATAATTTAGGA+AGG - chr1_1:77734590-77734609 None:intergenic 15.0%
!!! TATTTTGCTTTTTGTAAAAA+TGG + chr1_1:77735407-77735426 Msa0045780:intron 15.0%
!!! ATTTTGCTTTTTGTAAAAAT+GGG + chr1_1:77735408-77735427 Msa0045780:intron 15.0%
!!! TTTTTTTAAGAAGCTATTAA+TGG + chr1_1:77735799-77735818 Msa0045780:intron 15.0%
!! AAAAATTAAAAGAAAACAAG+AGG - chr1_1:77736998-77737017 None:intergenic 15.0%
!!! AATATTAGATTTTTCATTCA+AGG - chr1_1:77737071-77737090 None:intergenic 15.0%
!! TAATAATAATTTAGGAAGGA+AGG - chr1_1:77734586-77734605 None:intergenic 20.0%
!!! TATTTTGAATGAATTGACTT+TGG + chr1_1:77735442-77735461 Msa0045780:intron 20.0%
!!! TTTTTTTTGGAACATATTCT+TGG + chr1_1:77735639-77735658 Msa0045780:intron 20.0%
!! AATTTAATCAGATATACTTC+TGG - chr1_1:77735687-77735706 None:intergenic 20.0%
!! TCATAATTGAGTTTCTTTAT+TGG - chr1_1:77735764-77735783 None:intergenic 20.0%
!! TAATACTACTACTACAAATA+TGG - chr1_1:77736572-77736591 None:intergenic 20.0%
!! GCAACTACTTAAATAAATAA+AGG - chr1_1:77736800-77736819 None:intergenic 20.0%
!!! TGTTATTTTGCTTTGAAAAT+TGG + chr1_1:77736874-77736893 Msa0045780:intron 20.0%
! TTACAGTGAAGTAAAAAGAT+GGG - chr1_1:77734727-77734746 None:intergenic 25.0%
! TTTACAGTGAAGTAAAAAGA+TGG - chr1_1:77734728-77734747 None:intergenic 25.0%
!!! TGATATTGATTCTCAGATAA+TGG + chr1_1:77735060-77735079 Msa0045780:CDS 25.0%
!!! GATATTGATTCTCAGATAAT+GGG + chr1_1:77735061-77735080 Msa0045780:CDS 25.0%
! AACATCAAAACAAAGGAAAA+AGG - chr1_1:77735368-77735387 None:intergenic 25.0%
!!! TTTTTCCTTTGTTTTGATGT+TGG + chr1_1:77735367-77735386 Msa0045780:intron 25.0%
!!! TTTTCCTTTGTTTTGATGTT+GGG + chr1_1:77735368-77735387 Msa0045780:intron 25.0%
! AAGGTAAAACGATTTATGTT+TGG + chr1_1:77735479-77735498 Msa0045780:intron 25.0%
! AGGTAAAACGATTTATGTTT+GGG + chr1_1:77735480-77735499 Msa0045780:intron 25.0%
! TTGAGTATTTATCTTGCTTA+AGG + chr1_1:77735735-77735754 Msa0045780:intron 25.0%
! CTCAATTATGATGCTATTAA+TGG + chr1_1:77735773-77735792 Msa0045780:intron 25.0%
! ATTAACTTACTCGAACAAAT+CGG + chr1_1:77736522-77736541 Msa0045780:intron 25.0%
! TTTAAGTAGTTGCAAGTATA+AGG + chr1_1:77736807-77736826 Msa0045780:intron 25.0%
! AAACATGTTATATGAGACAA+GGG + chr1_1:77736940-77736959 Msa0045780:intron 25.0%
!! TCAATTTTGTCTAGAAGTTT+TGG + chr1_1:77736971-77736990 Msa0045780:intron 25.0%
!! CAATTTTGTCTAGAAGTTTT+GGG + chr1_1:77736972-77736991 Msa0045780:intron 25.0%
!!! ATTGTTGTTTCTCAATTTCT+TGG + chr1_1:77737088-77737107 Msa0045780:intron 25.0%
!! TATATTGTGGACATTTTTGT+TGG - chr1_1:77737362-77737381 None:intergenic 25.0%
! GTTATAGGATCATAACAAAT+TGG + chr1_1:77738181-77738200 Msa0045780:three_prime_UTR 25.0%
! TATGCATTTGTATAAATCAC+AGG + chr1_1:77738263-77738282 Msa0045780:three_prime_UTR 25.0%
! TTGTAACACAAAAATATTCC+TGG + chr1_1:77738340-77738359 Msa0045780:three_prime_UTR 25.0%
TGATAAAGAGAAAAGATGAG+TGG - chr1_1:77734502-77734521 None:intergenic 30.0%
!! TAATAAGTGTTGGCTTTGAT+TGG - chr1_1:77734535-77734554 None:intergenic 30.0%
TACAGTGAAGTAAAAAGATG+GGG - chr1_1:77734726-77734745 None:intergenic 30.0%
ACTCAAAGAGATTCAGAAAA+AGG + chr1_1:77734815-77734834 Msa0045780:CDS 30.0%
TCTGAGAATCAATATCAAGA+AGG - chr1_1:77735057-77735076 None:intergenic 30.0%
! GTTTCTAAAAAGAATCGATC+TGG + chr1_1:77735253-77735272 Msa0045780:CDS 30.0%
AAATCCCAACATCAAAACAA+AGG - chr1_1:77735375-77735394 None:intergenic 30.0%
!!! TTGTTTTGATGTTGGGATTT+TGG + chr1_1:77735375-77735394 Msa0045780:intron 30.0%
!! TTTGGCTAAAATTGAGTTGA+AGG + chr1_1:77735460-77735479 Msa0045780:intron 30.0%
ACATAGCATTAACTCTACTA+CGG - chr1_1:77735870-77735889 None:intergenic 30.0%
AATAAGACAACGTTGATGTT+TGG - chr1_1:77735904-77735923 None:intergenic 30.0%
! AACATCAACGTTGTCTTATT+TGG + chr1_1:77735904-77735923 Msa0045780:intron 30.0%
AGATTACTACAAAGAACAGA+AGG - chr1_1:77736000-77736019 None:intergenic 30.0%
ATCTAGTAAAACCTGAACAA+TGG - chr1_1:77736044-77736063 None:intergenic 30.0%
!! CAATAGTTTTGCTTAAGCTT+GGG - chr1_1:77736072-77736091 None:intergenic 30.0%
!!! ATGCAAAGCATTTTTTCCAT+TGG - chr1_1:77736200-77736219 None:intergenic 30.0%
TCATCACTATATGTACTGTA+TGG - chr1_1:77736492-77736511 None:intergenic 30.0%
TTAACTTACTCGAACAAATC+GGG + chr1_1:77736523-77736542 Msa0045780:intron 30.0%
AAGTCTCCAATATTGAAAAC+AGG + chr1_1:77736609-77736628 Msa0045780:intron 30.0%
! TAGCTACCTGTTTTCAATAT+TGG - chr1_1:77736618-77736637 None:intergenic 30.0%
GTGCACACTACTTAAAAAAA+TGG - chr1_1:77736910-77736929 None:intergenic 30.0%
GAAACATGTTATATGAGACA+AGG + chr1_1:77736939-77736958 Msa0045780:intron 30.0%
! AAGATTTTCAGCAATGTCAA+GGG - chr1_1:77737185-77737204 None:intergenic 30.0%
TGAAGAGTCATCAGATATAA+AGG + chr1_1:77737213-77737232 Msa0045780:CDS 30.0%
AATTAACAATGCCACAAACT+CGG + chr1_1:77737414-77737433 Msa0045780:CDS 30.0%
!! TATTGCGCTTTTTACATCTT+TGG + chr1_1:77737570-77737589 Msa0045780:CDS 30.0%
! TTTAGTTTCACCTCTAACAA+GGG - chr1_1:77737617-77737636 None:intergenic 30.0%
TGAAACTAAAGCAGAGAAAA+GGG + chr1_1:77737627-77737646 Msa0045780:CDS 30.0%
TTATCCTTTCCAACGTAATT+TGG - chr1_1:77737961-77737980 None:intergenic 30.0%
ATCTATGTAAACTCTTGAAG+TGG - chr1_1:77738018-77738037 None:intergenic 30.0%
ATCTATGTAAACTCTTGAAG+TGG - chr1_1:77738018-77738037 None:intergenic 30.0%
GATTTGTGAGAAGAATAACA+CGG + chr1_1:77738290-77738309 Msa0045780:three_prime_UTR 30.0%
AAAATTCTCAGTAACAGACT+AGG - chr1_1:77738378-77738397 None:intergenic 30.0%
! TTTTGCTACAAGAAATGTCT+TGG + chr1_1:77738394-77738413 Msa0045780:three_prime_UTR 30.0%
AAAGAGAAAAGATGAGTGGA+AGG - chr1_1:77734498-77734517 None:intergenic 35.0%
GCATGAGTCATAATAAGTGT+TGG - chr1_1:77734545-77734564 None:intergenic 35.0%
ACAGTGAAGTAAAAAGATGG+GGG - chr1_1:77734725-77734744 None:intergenic 35.0%
! TACGCTTTTACGTTTATTGC+AGG + chr1_1:77734782-77734801 Msa0045780:intron 35.0%
TCCTTTCAAACTCAGGAAAA+CGG + chr1_1:77734921-77734940 Msa0045780:CDS 35.0%
GTGAATGAAGAGAATGAACT+TGG + chr1_1:77735148-77735167 Msa0045780:CDS 35.0%
TTTGTTTACTGCTGCTGAAA+AGG + chr1_1:77735180-77735199 Msa0045780:CDS 35.0%
TTGTTTACTGCTGCTGAAAA+GGG + chr1_1:77735181-77735200 Msa0045780:CDS 35.0%
GCTTCAAGTAGAATCAGTTA+TGG + chr1_1:77735578-77735597 Msa0045780:intron 35.0%
TCAGTTATGGAGACAAAATC+AGG + chr1_1:77735591-77735610 Msa0045780:intron 35.0%
CTTGAGTAGCTTCAATTTGT+GGG + chr1_1:77735943-77735962 Msa0045780:intron 35.0%
! TAGCTTCAATTTGTGGGTTA+TGG + chr1_1:77735949-77735968 Msa0045780:intron 35.0%
!! CCAATAGTTTTGCTTAAGCT+TGG - chr1_1:77736073-77736092 None:intergenic 35.0%
CCAAGCTTAAGCAAAACTAT+TGG + chr1_1:77736070-77736089 Msa0045780:CDS 35.0%
AATGAACTTCTGTCAAAGGA+TGG + chr1_1:77736151-77736170 Msa0045780:CDS 35.0%
TTGGAAATTGCAAGATCCAA+TGG + chr1_1:77736181-77736200 Msa0045780:CDS 35.0%
CATTGCATATGGCTGTAAAA+GGG + chr1_1:77736314-77736333 Msa0045780:CDS 35.0%
GTTATGCTTCCTGACAAATT+TGG + chr1_1:77736385-77736404 Msa0045780:CDS 35.0%
TGCTACAAAAAATGCAAACG+AGG + chr1_1:77736642-77736661 Msa0045780:intron 35.0%
AAGTAGTTGCAAGTATAAGG+TGG + chr1_1:77736810-77736829 Msa0045780:intron 35.0%
ACATGCTCAAAAGTAACTAG+GGG - chr1_1:77737041-77737060 None:intergenic 35.0%
GACATGCTCAAAAGTAACTA+GGG - chr1_1:77737042-77737061 None:intergenic 35.0%
TGACATGCTCAAAAGTAACT+AGG - chr1_1:77737043-77737062 None:intergenic 35.0%
AATGCATTTACGTTTGTGTC+TGG - chr1_1:77737142-77737161 None:intergenic 35.0%
! GAAGATTTTCAGCAATGTCA+AGG - chr1_1:77737186-77737205 None:intergenic 35.0%
!!! GACATTTTTGTTGGTTCTCT+TGG - chr1_1:77737353-77737372 None:intergenic 35.0%
TCAGCTCTTTGGATATATTG+TGG - chr1_1:77737375-77737394 None:intergenic 35.0%
ACAATATATCCAAAGAGCTG+AGG + chr1_1:77737374-77737393 Msa0045780:CDS 35.0%
! TTTTACATCTTTGGCTGTTG+TGG + chr1_1:77737579-77737598 Msa0045780:CDS 35.0%
GTTCAAATTACCCTTGTTAG+AGG + chr1_1:77737604-77737623 Msa0045780:CDS 35.0%
! CTTTAGTTTCACCTCTAACA+AGG - chr1_1:77737618-77737637 None:intergenic 35.0%
GTGAAACTAAAGCAGAGAAA+AGG + chr1_1:77737626-77737645 Msa0045780:CDS 35.0%
GTGATAATATCCGGAGTTAT+TGG + chr1_1:77737781-77737800 Msa0045780:CDS 35.0%
CTATGACTTACTATGTAGTG+AGG + chr1_1:77737806-77737825 Msa0045780:CDS 35.0%
GTTTGAAGACCAAATTACGT+TGG + chr1_1:77737949-77737968 Msa0045780:three_prime_UTR 35.0%
AAGACCAAATTACGTTGGAA+AGG + chr1_1:77737954-77737973 Msa0045780:three_prime_UTR 35.0%
! TAAGCTGCTGTTGTTGTTAT+AGG + chr1_1:77738166-77738185 Msa0045780:three_prime_UTR 35.0%
ACTAGGATAACATATAGACC+AGG - chr1_1:77738361-77738380 None:intergenic 35.0%
AAAGGAAAGAGGGAGGAAAT+GGG - chr1_1:77734475-77734494 None:intergenic 40.0%
GAAAAGATGAGTGGAAGGAA+AGG - chr1_1:77734493-77734512 None:intergenic 40.0%
GTCATGATAGAAGAGTAGAG+GGG - chr1_1:77734660-77734679 None:intergenic 40.0%
GGTCATGATAGAAGAGTAGA+GGG - chr1_1:77734661-77734680 None:intergenic 40.0%
AGGTCATGATAGAAGAGTAG+AGG - chr1_1:77734662-77734681 None:intergenic 40.0%
AGATAGAAGAAGAACCAACC+TGG - chr1_1:77734699-77734718 None:intergenic 40.0%
! GCTTTTACGTTTATTGCAGG+TGG + chr1_1:77734785-77734804 Msa0045780:intron 40.0%
ACGGAGATATTTCGGAAAGT+GGG - chr1_1:77734865-77734884 None:intergenic 40.0%
! TCCGTTTTCCTGAGTTTGAA+AGG - chr1_1:77734925-77734944 None:intergenic 40.0%
GAAGTACGTGAAGCAAGTAA+CGG + chr1_1:77734964-77734983 Msa0045780:CDS 40.0%
AAGTACGTGAAGCAAGTAAC+GGG + chr1_1:77734965-77734984 Msa0045780:CDS 40.0%
!! CAGATAATGGGTACTTTGAG+TGG + chr1_1:77735073-77735092 Msa0045780:CDS 40.0%
GGGTCATCTTGATGTTGTTA+AGG + chr1_1:77735201-77735220 Msa0045780:CDS 40.0%
TGACTAGCAGCAATATGCAA+AGG - chr1_1:77735286-77735305 None:intergenic 40.0%
TTGCATATTGCTGCTAGTCA+AGG + chr1_1:77735286-77735305 Msa0045780:CDS 40.0%
GCTACTCAAGGTCTAACAAT+TGG - chr1_1:77735933-77735952 None:intergenic 40.0%
CCACAAATTGAAGCTACTCA+AGG - chr1_1:77735945-77735964 None:intergenic 40.0%
CCTTGAGTAGCTTCAATTTG+TGG + chr1_1:77735942-77735961 Msa0045780:intron 40.0%
TTCATTTGCAGCCATTGTTC+AGG + chr1_1:77736030-77736049 Msa0045780:intron 40.0%
GGTGAATGAACTTCTGTCAA+AGG + chr1_1:77736147-77736166 Msa0045780:CDS 40.0%
AGGCTTTCACGATTTCTGTA+TGG - chr1_1:77736240-77736259 None:intergenic 40.0%
GCATTGCATATGGCTGTAAA+AGG + chr1_1:77736313-77736332 Msa0045780:CDS 40.0%
CATTGTTAATTCCTTCCCTG+TGG - chr1_1:77737405-77737424 None:intergenic 40.0%
AGGTGATTAACAAGCTCATG+TGG + chr1_1:77737656-77737675 Msa0045780:CDS 40.0%
GATTAACAAGCTCATGTGGT+TGG + chr1_1:77737660-77737679 Msa0045780:CDS 40.0%
! GCAATTCTGGTTACAGTTGT+TGG + chr1_1:77737754-77737773 Msa0045780:CDS 40.0%
! ATTCTGGTTACAGTTGTTGG+AGG + chr1_1:77737757-77737776 Msa0045780:CDS 40.0%
GTTGGAGGAGTGATAATATC+CGG + chr1_1:77737772-77737791 Msa0045780:CDS 40.0%
AGTCATAGTGCCAATAACTC+CGG - chr1_1:77737794-77737813 None:intergenic 40.0%
CTAAGAGAACAAGATCCATG+AGG + chr1_1:77737830-77737849 Msa0045780:CDS 40.0%
AATTCGATCAACCTCGGAAT+TGG - chr1_1:77737917-77737936 None:intergenic 40.0%
CGCATAAATTCGATCAACCT+CGG - chr1_1:77737923-77737942 None:intergenic 40.0%
TGCTACAAGAAATGTCTTGG+AGG + chr1_1:77738397-77738416 Msa0045780:three_prime_UTR 40.0%
GCTACAAGAAATGTCTTGGA+GGG + chr1_1:77738398-77738417 Msa0045780:three_prime_UTR 40.0%
AAAGTCGTGAGAAAGAGGGA+GGG - chr1_1:77734452-77734471 None:intergenic 45.0%
TGGGAAAGTCGTGAGAAAGA+GGG - chr1_1:77734456-77734475 None:intergenic 45.0%
ATGGGAAAGTCGTGAGAAAG+AGG - chr1_1:77734457-77734476 None:intergenic 45.0%
GAAAGGAAAGAGGGAGGAAA+TGG - chr1_1:77734476-77734495 None:intergenic 45.0%
GAGTGGAAGGAAAGGAAAGA+GGG - chr1_1:77734485-77734504 None:intergenic 45.0%
TGAGTGGAAGGAAAGGAAAG+AGG - chr1_1:77734486-77734505 None:intergenic 45.0%
GGAAGGAAGGATGAAAGTGA+AGG - chr1_1:77734573-77734592 None:intergenic 45.0%
ACCTCCAATTTCAACCTTCC+AGG + chr1_1:77734678-77734697 Msa0045780:CDS 45.0%
ACCTGGAAGGTTGAAATTGG+AGG - chr1_1:77734682-77734701 None:intergenic 45.0%
CCAACCTGGAAGGTTGAAAT+TGG - chr1_1:77734685-77734704 None:intergenic 45.0%
CCAATTTCAACCTTCCAGGT+TGG + chr1_1:77734682-77734701 Msa0045780:intron 45.0%
AGAAGAAGAACCAACCTGGA+AGG - chr1_1:77734695-77734714 None:intergenic 45.0%
GACGGAGATATTTCGGAAAG+TGG - chr1_1:77734866-77734885 None:intergenic 45.0%
AGATGGCGACGGAGATATTT+CGG - chr1_1:77734873-77734892 None:intergenic 45.0%
GAGTTTGAAAGGACTAGAGC+CGG - chr1_1:77734914-77734933 None:intergenic 45.0%
GCTCTAGTCCTTTCAAACTC+AGG + chr1_1:77734914-77734933 Msa0045780:CDS 45.0%
GAAAACGGATCGATCAAGCA+GGG + chr1_1:77734936-77734955 Msa0045780:CDS 45.0%
CTTCATCTAGCAGCTCAAAG+AGG + chr1_1:77735007-77735026 Msa0045780:CDS 45.0%
TGTTATAAGTGGAGTGGCGT+TGG - chr1_1:77736101-77736120 None:intergenic 45.0%
TTATAACAGCAGCGACGAGA+GGG + chr1_1:77736113-77736132 Msa0045780:CDS 45.0%
TTTGCATTTAGCTGCTCGTC+CGG + chr1_1:77736213-77736232 Msa0045780:CDS 45.0%
TTCACGATTTCTGTATGGCC+CGG - chr1_1:77736235-77736254 None:intergenic 45.0%
AGGGCAAACTGCATTGCATA+TGG + chr1_1:77736303-77736322 Msa0045780:CDS 45.0%
GCTGTGTTGCCAAATTTGTC+AGG - chr1_1:77736397-77736416 None:intergenic 45.0%
AACCAGGAAAAAGCGAGTAG+AGG + chr1_1:77736429-77736448 Msa0045780:CDS 45.0%
!!! TACCTCTACTCGCTTTTTCC+TGG - chr1_1:77736434-77736453 None:intergenic 45.0%
CATTTACGTTTGTGTCTGGC+AGG - chr1_1:77737138-77737157 None:intergenic 45.0%
!! CAATGTCAAGGGCTGTTTTG+TGG - chr1_1:77737174-77737193 None:intergenic 45.0%
TCTGATGACTCTTCAGAGAG+AGG - chr1_1:77737208-77737227 None:intergenic 45.0%
AAGGACATCCTCTCTCGATA+TGG + chr1_1:77737232-77737251 Msa0045780:CDS 45.0%
AGGACATCCTCTCTCGATAT+GGG + chr1_1:77737233-77737252 Msa0045780:CDS 45.0%
TAACGAGCTCAACCAACCAA+GGG + chr1_1:77737267-77737286 Msa0045780:CDS 45.0%
AAGGGACGAACTGAGGAAAA+CGG + chr1_1:77737285-77737304 Msa0045780:CDS 45.0%
GAAAGATGTTCACACACAGC+TGG + chr1_1:77737321-77737340 Msa0045780:CDS 45.0%
CTATATTCACCGTACCTGGT+GGG + chr1_1:77737479-77737498 Msa0045780:CDS 45.0%
TATATTCACCGTACCTGGTG+GGG + chr1_1:77737480-77737499 Msa0045780:CDS 45.0%
!! TAACGATGATGGATCAGGAG+TGG + chr1_1:77737504-77737523 Msa0045780:CDS 45.0%
AGCAGAGAAAAGGGTAGTTG+AGG + chr1_1:77737636-77737655 Msa0045780:CDS 45.0%
CTGCTTCTCTTTCTTCCTCA+TGG - chr1_1:77737848-77737867 None:intergenic 45.0%
CTCTGAATGATGCCATGAGT+TGG - chr1_1:77737890-77737909 None:intergenic 45.0%
TTCAGAGTTCTCCAATTCCG+AGG + chr1_1:77737903-77737922 Msa0045780:CDS 45.0%
!! GAGTTTTGTGCACTTCAGCA+TGG + chr1_1:77738234-77738253 Msa0045780:intron 45.0%
AAGTCGTGAGAAAGAGGGAG+GGG - chr1_1:77734451-77734470 None:intergenic 50.0%
GAAAGTCGTGAGAAAGAGGG+AGG - chr1_1:77734453-77734472 None:intergenic 50.0%
TGGAAGGAAAGGAAAGAGGG+AGG - chr1_1:77734482-77734501 None:intergenic 50.0%
! TCGGAAAGTGGGTTCTGTGT+TGG - chr1_1:77734854-77734873 None:intergenic 50.0%
GGAAAACGGATCGATCAAGC+AGG + chr1_1:77734935-77734954 Msa0045780:CDS 50.0%
GCAGCTCAAAGAGGTGATGT+TGG + chr1_1:77735016-77735035 Msa0045780:CDS 50.0%
GCTGCTAGTCAAGGACATCA+TGG + chr1_1:77735295-77735314 Msa0045780:CDS 50.0%
! ATAAGTGGAGTGGCGTTGGA+TGG - chr1_1:77736097-77736116 None:intergenic 50.0%
CGCTGCTGTTATAAGTGGAG+TGG - chr1_1:77736107-77736126 None:intergenic 50.0%
CTCGTCGCTGCTGTTATAAG+TGG - chr1_1:77736112-77736131 None:intergenic 50.0%
CTTATAACAGCAGCGACGAG+AGG + chr1_1:77736112-77736131 Msa0045780:CDS 50.0%
GTCAAAGGATGGCAGCTTGT+TGG + chr1_1:77736162-77736181 Msa0045780:CDS 50.0%
TTGCATTTAGCTGCTCGTCC+GGG + chr1_1:77736214-77736233 Msa0045780:CDS 50.0%
TTGAGGGTCTTTGCTGAGCA+AGG - chr1_1:77736260-77736279 None:intergenic 50.0%
GCTCAGCAAAGACCCTCAAT+TGG + chr1_1:77736261-77736280 Msa0045780:CDS 50.0%
GCAAAGACCCTCAATTGGCT+CGG + chr1_1:77736266-77736285 Msa0045780:CDS 50.0%
CTCGGAGGACTGACAAGAAA+GGG + chr1_1:77736284-77736303 Msa0045780:CDS 50.0%
TGGCAACACAGCATTGCATG+TGG + chr1_1:77736405-77736424 Msa0045780:CDS 50.0%
GGACATCCTCTCTCGATATG+GGG + chr1_1:77737234-77737253 Msa0045780:CDS 50.0%
TAAGTGCCCCATATCGAGAG+AGG - chr1_1:77737243-77737262 None:intergenic 50.0%
CTAACGAGCTCAACCAACCA+AGG + chr1_1:77737266-77737285 Msa0045780:CDS 50.0%
! GTTTTCCTCAGTTCGTCCCT+TGG - chr1_1:77737286-77737305 None:intergenic 50.0%
GTGGAGTTTCCTCAGCTCTT+TGG - chr1_1:77737386-77737405 None:intergenic 50.0%
AAGAGCTGAGGAAACTCCAC+AGG + chr1_1:77737386-77737405 Msa0045780:CDS 50.0%
AGAGCTGAGGAAACTCCACA+GGG + chr1_1:77737387-77737406 Msa0045780:CDS 50.0%
GCTGCTATATTCACCGTACC+TGG + chr1_1:77737475-77737494 Msa0045780:CDS 50.0%
GCTATATTCACCGTACCTGG+TGG + chr1_1:77737478-77737497 Msa0045780:CDS 50.0%
ATCGTTATCCCCACCAGGTA+CGG - chr1_1:77737491-77737510 None:intergenic 50.0%
!! GGGGATAACGATGATGGATC+AGG + chr1_1:77737499-77737518 Msa0045780:CDS 50.0%
! GTTGGCTTCTGTCTGTACCT+CGG + chr1_1:77737678-77737697 Msa0045780:CDS 50.0%
AGAGGCAATAAATGCCACCG+AGG - chr1_1:77737698-77737717 None:intergenic 50.0%
GCCTCTTCGTACATAGTCGT+CGG + chr1_1:77737712-77737731 Msa0045780:CDS 50.0%
ACCGACGACTATGTACGAAG+AGG - chr1_1:77737716-77737735 None:intergenic 50.0%
CTTCGTACATAGTCGTCGGT+CGG + chr1_1:77737716-77737735 Msa0045780:CDS 50.0%
AGAAAGAGAAGCAGCTAGCC+AGG + chr1_1:77737854-77737873 Msa0045780:CDS 50.0%
! TGAGTTGGATCCACTTCTCC+TGG - chr1_1:77737875-77737894 None:intergenic 50.0%
GGAGAAGTGGATCCAACTCA+TGG + chr1_1:77737875-77737894 Msa0045780:CDS 50.0%
!! AAGACCCTCAATTGGCTCGG+AGG + chr1_1:77736269-77736288 Msa0045780:CDS 55.0%
CAGTCCTCCGAGCCAATTGA+GGG - chr1_1:77736276-77736295 None:intergenic 55.0%
TCAGTCCTCCGAGCCAATTG+AGG - chr1_1:77736277-77736296 None:intergenic 55.0%
GCTCGGAGGACTGACAAGAA+AGG + chr1_1:77736283-77736302 Msa0045780:CDS 55.0%
AAAAGGGCAGAGCTGTGACG+TGG + chr1_1:77736330-77736349 Msa0045780:CDS 55.0%
CAGCATTGCATGTGGCAACC+AGG + chr1_1:77736413-77736432 Msa0045780:CDS 55.0%
ACCAACCAAGGGACGAACTG+AGG + chr1_1:77737278-77737297 Msa0045780:CDS 55.0%
TCCTCAGTTCGTCCCTTGGT+TGG - chr1_1:77737282-77737301 None:intergenic 55.0%
CTGAGGAAACTCCACAGGGA+AGG + chr1_1:77737391-77737410 Msa0045780:CDS 55.0%
! TGCCACAAACTCGGTGACAG+TGG + chr1_1:77737423-77737442 Msa0045780:CDS 55.0%
CACCACTGTCACCGAGTTTG+TGG - chr1_1:77737428-77737447 None:intergenic 55.0%
! CACAAACTCGGTGACAGTGG+TGG + chr1_1:77737426-77737445 Msa0045780:CDS 55.0%
CCATCATCGTTATCCCCACC+AGG - chr1_1:77737496-77737515 None:intergenic 55.0%
CCTGGTGGGGATAACGATGA+TGG + chr1_1:77737493-77737512 Msa0045780:CDS 55.0%
!! GAACGAGTGGGCTGCAATTC+TGG + chr1_1:77737741-77737760 Msa0045780:CDS 55.0%
AAGCAGCTAGCCAGGAGAAG+TGG + chr1_1:77737862-77737881 Msa0045780:CDS 55.0%
GGCTTCTGTCTGTACCTCGG+TGG + chr1_1:77737681-77737700 Msa0045780:CDS 60.0%
CGTACATAGTCGTCGGTCGG+AGG + chr1_1:77737719-77737738 Msa0045780:CDS 60.0%
! GACGAGAGGGCACGTCGAAG+TGG + chr1_1:77736126-77736145 Msa0045780:CDS 65.0%
TCGTCGGTCGGAGGAACGAG+TGG + chr1_1:77737728-77737747 Msa0045780:CDS 65.0%
CGTCGGTCGGAGGAACGAGT+GGG + chr1_1:77737729-77737748 Msa0045780:CDS 65.0%
GCGGACGACGGAGATGGCGA+CGG - chr1_1:77734884-77734903 None:intergenic 70.0%
GCCATCTCCGTCGTCCGCGA+CGG + chr1_1:77734886-77734905 Msa0045780:CDS 70.0%
GCCGTCGCGGACGACGGAGA+TGG - chr1_1:77734890-77734909 None:intergenic 75.0%
GACTAGAGCCGGCGCCGTCG+CGG - chr1_1:77734903-77734922 None:intergenic 75.0%
! TCCGTCGTCCGCGACGGCGC+CGG + chr1_1:77734892-77734911 Msa0045780:CDS 80.0%
! GCCGGCGCCGTCGCGGACGA+CGG - chr1_1:77734896-77734915 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 77734441 77738423 77734441 ID=Msa0045780;Name=Msa0045780
chr1_1 mRNA 77734441 77738423 77734441 ID=Msa0045780-mRNA-1;Parent=Msa0045780;Name=Msa0045780-mRNA-1;_AED=0.04;_eAED=0.04;_QI=234|1|1|1|0.75|0.6|5|455|593
chr1_1 exon 77734441 77734699 77734441 ID=Msa0045780-mRNA-1:exon:26601;Parent=Msa0045780-mRNA-1
chr1_1 exon 77734804 77735316 77734804 ID=Msa0045780-mRNA-1:exon:26602;Parent=Msa0045780-mRNA-1
chr1_1 exon 77736041 77736450 77736041 ID=Msa0045780-mRNA-1:exon:26603;Parent=Msa0045780-mRNA-1
chr1_1 exon 77737115 77738231 77737115 ID=Msa0045780-mRNA-1:exon:26604;Parent=Msa0045780-mRNA-1
chr1_1 exon 77738252 77738423 77738252 ID=Msa0045780-mRNA-1:exon:26605;Parent=Msa0045780-mRNA-1
chr1_1 five_prime_UTR 77734441 77734674 77734441 ID=Msa0045780-mRNA-1:five_prime_utr;Parent=Msa0045780-mRNA-1
chr1_1 CDS 77734675 77734699 77734675 ID=Msa0045780-mRNA-1:cds;Parent=Msa0045780-mRNA-1
chr1_1 CDS 77734804 77735316 77734804 ID=Msa0045780-mRNA-1:cds;Parent=Msa0045780-mRNA-1
chr1_1 CDS 77736041 77736450 77736041 ID=Msa0045780-mRNA-1:cds;Parent=Msa0045780-mRNA-1
chr1_1 CDS 77737115 77737948 77737115 ID=Msa0045780-mRNA-1:cds;Parent=Msa0045780-mRNA-1
chr1_1 three_prime_UTR 77737949 77738231 77737949 ID=Msa0045780-mRNA-1:three_prime_utr;Parent=Msa0045780-mRNA-1
chr1_1 three_prime_UTR 77738252 77738423 77738252 ID=Msa0045780-mRNA-1:three_prime_utr;Parent=Msa0045780-mRNA-1
Gene Sequence

>Msa0045780

ATGACCTCCAATTTCAACCTTCCAGGTGGAGCTCAAACTCAAAGAGATTCAGAAAAAGGAACGATGATGACACCAACACAGAACCCACTTTCCGAAATATCTCCGTCGCCATCTCCGTCGTCCGCGACGGCGCCGGCTCTAGTCCTTTCAAACTCAGGAAAACGGATCGATCAAGCAGGGAAGAAGAAGTACGTGAAGCAAGTAACGGGTCGTCACAACGACACAGAGCTTCATCTAGCAGCTCAAAGAGGTGATGTTGGTGCTGTGAGACAGATCCTTCTTGATATTGATTCTCAGATAATGGGTACTTTGAGTGGTGATGATGATGTTGATCTTAACGCTGAGATTGCTGAAGTTCGTGCTCTTGTTGTGAATGAAGAGAATGAACTTGGTGAAACTGCTTTGTTTACTGCTGCTGAAAAGGGTCATCTTGATGTTGTTAAGGAGTTGTTGAAACATTCAACTCTTCAAACTGTTTCTAAAAAGAATCGATCTGGTTTTGATCCTTTGCATATTGCTGCTAGTCAAGGACATCATGCCATTGTTCAGGTTTTACTAGATTATGACCCAAGCTTAAGCAAAACTATTGGTCCATCCAACGCCACTCCACTTATAACAGCAGCGACGAGAGGGCACGTCGAAGTGGTGAATGAACTTCTGTCAAAGGATGGCAGCTTGTTGGAAATTGCAAGATCCAATGGAAAAAATGCTTTGCATTTAGCTGCTCGTCCGGGCCATACAGAAATCGTGAAAGCCTTGCTCAGCAAAGACCCTCAATTGGCTCGGAGGACTGACAAGAAAGGGCAAACTGCATTGCATATGGCTGTAAAAGGGCAGAGCTGTGACGTGGTGAAATTGCTTCTTGATGCAGATGCTGCTATTGTTATGCTTCCTGACAAATTTGGCAACACAGCATTGCATGTGGCAACCAGGAAAAAGCGAGTAGAGATAGTGAATGAGTTGTTACTCCTGCCAGACACAAACGTAAATGCATTAACACGAGACCACAAAACAGCCCTTGACATTGCTGAAAATCTTCCTCTCTCTGAAGAGTCATCAGATATAAAGGACATCCTCTCTCGATATGGGGCACTTAGAGCTAACGAGCTCAACCAACCAAGGGACGAACTGAGGAAAACGGTTACACAAATTAAGAAAGATGTTCACACACAGCTGGAACAAACCAAGAGAACCAACAAAAATGTCCACAATATATCCAAAGAGCTGAGGAAACTCCACAGGGAAGGAATTAACAATGCCACAAACTCGGTGACAGTGGTGGCTGTGTTGTTTGCTACAGTTGCTTTTGCTGCTATATTCACCGTACCTGGTGGGGATAACGATGATGGATCAGGAGTGGTAGCGAGTTATTCTGCTTTTAAAATTTTCTTCATATTCAATGCTATTGCGCTTTTTACATCTTTGGCTGTTGTGGTTGTTCAAATTACCCTTGTTAGAGGTGAAACTAAAGCAGAGAAAAGGGTAGTTGAGGTGATTAACAAGCTCATGTGGTTGGCTTCTGTCTGTACCTCGGTGGCATTTATTGCCTCTTCGTACATAGTCGTCGGTCGGAGGAACGAGTGGGCTGCAATTCTGGTTACAGTTGTTGGAGGAGTGATAATATCCGGAGTTATTGGCACTATGACTTACTATGTAGTGAGGTCTAAGAGAACAAGATCCATGAGGAAGAAAGAGAAGCAGCTAGCCAGGAGAAGTGGATCCAACTCATGGCATCATTCAGAGTTCTCCAATTCCGAGGTTGATCGAATTTATGCGCTCTAA
Protein sequence

>Msa0045780

MTSNFNLPGGAQTQRDSEKGTMMTPTQNPLSEISPSPSPSSATAPALVLSNSGKRIDQAGKKKYVKQVTGRHNDTELHLAAQRGDVGAVRQILLDIDSQIMGTLSGDDDVDLNAEIAEVRALVVNEENELGETALFTAAEKGHLDVVKELLKHSTLQTVSKKNRSGFDPLHIAASQGHHAIVQVLLDYDPSLSKTIGPSNATPLITAATRGHVEVVNELLSKDGSLLEIARSNGKNALHLAARPGHTEIVKALLSKDPQLARRTDKKGQTALHMAVKGQSCDVVKLLLDADAAIVMLPDKFGNTALHVATRKKRVEIVNELLLLPDTNVNALTRDHKTALDIAENLPLSEESSDIKDILSRYGALRANELNQPRDELRKTVTQIKKDVHTQLEQTKRTNKNVHNISKELRKLHREGINNATNSVTVVAVLFATVAFAAIFTVPGGDNDDGSGVVASYSAFKIFFIFNAIALFTSLAVVVVQITLVRGETKAEKRVVEVINKLMWLASVCTSVAFIASSYIVVGRRNEWAAILVTVVGGVIISGVIGTMTYYVVRSKRTRSMRKKEKQLARRSGSNSWHHSEFSNSEVDRIYAL*