AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1399470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1399470 MtrunA17_Chr1g0212971 99.157 593 5 0 1 593 1 593 0.0 1201
Msa1399470 MtrunA17_Chr7g0263471 73.042 549 141 4 46 593 40 582 0.0 791
Msa1399470 MtrunA17_Chr2g0327711 46.209 554 253 9 61 593 15 544 2.55e-147 436
Msa1399470 MtrunA17_Chr4g0030551 47.126 522 237 8 58 563 16 514 2.40e-145 431
Msa1399470 MtrunA17_Chr7g0276681 45.421 546 255 7 61 593 31 546 7.71e-137 409
Msa1399470 MtrunA17_Chr1g0161231 45.436 482 246 7 127 593 52 531 5.60e-128 386
Msa1399470 MtrunA17_Chr7g0263421 42.620 542 273 7 66 593 3 520 2.95e-125 379
Msa1399470 MtrunA17_Chr8g0391711 43.659 481 256 4 127 593 59 538 6.24e-125 378
Msa1399470 MtrunA17_Chr2g0331191 22.951 610 356 14 4 567 69 610 8.96e-27 115
Msa1399470 MtrunA17_Chr2g0331191 25.403 248 184 1 96 343 1 247 1.05e-20 96.3
Msa1399470 MtrunA17_Chr3g0136651 22.707 458 298 12 138 577 8 427 3.84e-25 108
Msa1399470 MtrunA17_Chr2g0331201 28.896 308 202 5 74 367 37 341 1.77e-21 99.0
Msa1399470 MtrunA17_Chr2g0331201 26.949 449 250 15 130 522 137 563 1.68e-20 95.9
Msa1399470 MtrunA17_Chr2g0331201 26.471 238 173 2 107 343 11 247 4.28e-19 91.3
Msa1399470 MtrunA17_Chr8g0361991 25.469 373 214 10 76 445 10 321 1.60e-19 91.7
Msa1399470 MtrunA17_Chr2g0328031 31.707 205 121 4 134 322 14 215 1.99e-19 91.3
Msa1399470 MtrunA17_Chr8g0370361 26.756 299 178 6 169 452 46 318 5.30e-19 90.1
Msa1399470 MtrunA17_Chr8g0367671 23.786 412 245 12 169 543 47 426 1.21e-18 89.0
Msa1399470 MtrunA17_Chr1g0178581 24.565 460 262 15 138 556 15 430 3.83e-18 87.4
Msa1399470 MtrunA17_Chr7g0233331 24.611 321 206 6 138 445 12 309 2.26e-16 81.6
Msa1399470 MtrunA17_Chr5g0439361 25.908 413 253 13 169 562 23 401 3.14e-16 81.3
Msa1399470 MtrunA17_Chr5g0439351 27.128 376 238 11 169 533 338 688 3.67e-16 82.0
Msa1399470 MtrunA17_Chr5g0439161 24.319 477 310 14 128 562 207 674 6.51e-16 81.3
Msa1399470 MtrunA17_Chr8g0369921 23.653 427 245 13 169 556 45 429 7.85e-16 80.1
Msa1399470 MtrunA17_Chr4g0031491 30.288 208 124 7 132 322 13 216 9.13e-16 80.1
Msa1399470 MtrunA17_Chr8g0368621 24.315 292 180 6 169 445 42 307 2.74e-15 77.4
Msa1399470 MtrunA17_Chr3g0100301 24.552 391 245 14 189 542 15 392 2.94e-15 78.2
Msa1399470 MtrunA17_Chr7g0262731 27.917 240 158 6 135 361 9 246 3.95e-15 78.6
Msa1399470 MtrunA17_Chr7g0221531 23.099 355 208 9 169 488 44 368 1.99e-14 75.5
Msa1399470 MtrunA17_Chr8g0354071 23.684 342 198 10 177 480 2 318 2.79e-14 75.1
Msa1399470 MtrunA17_Chr8g0364031 25.000 336 200 10 138 453 13 316 5.79e-14 74.3
Msa1399470 MtrunA17_Chr5g0418551 33.708 178 111 5 132 306 20 193 1.02e-13 71.2
Msa1399470 MtrunA17_Chr7g0263431 71.429 49 13 1 274 321 1 49 1.26e-13 65.5
Msa1399470 MtrunA17_Chr3g0093531 23.684 342 198 10 177 480 2 318 1.90e-13 72.4
Msa1399470 MtrunA17_Chr3g0083331 24.917 301 178 9 169 445 45 321 2.71e-13 72.4
Msa1399470 MtrunA17_Chr8g0353311 21.809 376 234 9 169 509 49 399 4.61e-13 71.6
Msa1399470 MtrunA17_Chr8g0376971 26.183 317 194 9 72 352 77 389 6.24e-13 71.6
Msa1399470 MtrunA17_Chr1g0211241 29.952 207 137 5 120 322 120 322 1.10e-12 70.9
Msa1399470 MtrunA17_Chr3g0122941 28.342 187 125 3 131 309 105 290 1.56e-12 70.5
Msa1399470 MtrunA17_Chr3g0122941 27.208 283 173 9 100 355 108 384 2.92e-12 69.7
Msa1399470 MtrunA17_Chr6g0461061 27.935 247 158 7 90 323 125 364 1.63e-11 67.0
Msa1399470 MtrunA17_Chr5g0410201 25.284 352 185 11 213 544 7 300 1.71e-11 65.9
Msa1399470 MtrunA17_Chr4g0064931 30.319 188 127 4 135 322 40 223 2.05e-11 67.0
Msa1399470 MtrunA17_Chr7g0262761 27.922 154 100 3 169 312 47 199 2.36e-11 63.5
Msa1399470 MtrunA17_Chr4g0073161 30.392 204 98 6 128 294 9 205 3.05e-11 66.2
Msa1399470 MtrunA17_Chr1g0196261 28.947 228 134 8 114 317 198 421 5.70e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1399470 AT3G12360.1 80.684 585 104 4 14 593 10 590 0.0 954
Msa1399470 AT3G12360.2 80.895 581 102 4 18 593 2 578 0.0 950
Msa1399470 AT3G09550.1 71.502 586 148 3 24 593 25 607 0.0 833
Msa1399470 AT3G09550.3 71.502 586 148 3 24 593 25 607 0.0 833
Msa1399470 AT3G09550.2 71.502 586 148 3 24 593 32 614 0.0 832
Msa1399470 AT2G31820.1 43.534 549 271 7 60 593 138 662 8.01e-138 416
Msa1399470 AT1G05640.1 44.404 554 270 7 56 593 96 627 7.62e-136 410
Msa1399470 AT5G60070.1 46.595 558 244 13 61 593 20 548 3.40e-135 405
Msa1399470 AT2G01680.1 43.110 508 273 8 99 593 28 532 2.25e-129 390
Msa1399470 AT1G07710.2 44.565 552 264 5 61 593 15 543 2.44e-129 390
Msa1399470 AT1G07710.1 44.565 552 264 5 61 593 15 543 2.44e-129 390
Msa1399470 AT5G02620.4 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa1399470 AT5G02620.1 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa1399470 AT5G02620.3 43.774 514 248 7 61 556 5 495 4.25e-124 376
Msa1399470 AT5G02620.5 41.899 537 248 8 61 556 5 518 1.15e-118 363
Msa1399470 AT5G02620.2 41.899 537 248 8 61 556 5 518 1.15e-118 363
Msa1399470 AT1G10340.1 25.202 496 309 12 111 552 51 538 2.25e-34 138
Msa1399470 AT5G54710.1 27.308 520 284 17 68 552 97 557 1.49e-33 135
Msa1399470 AT5G54710.2 27.308 520 284 17 68 552 142 602 2.33e-33 135
Msa1399470 AT1G10340.2 24.593 492 313 11 111 552 51 534 2.38e-33 135
Msa1399470 AT1G34050.1 26.145 524 294 15 108 554 28 535 6.31e-33 134
Msa1399470 AT2G24600.3 26.892 502 290 18 72 538 70 529 3.55e-32 131
Msa1399470 AT2G24600.4 26.892 502 290 18 72 538 70 529 3.55e-32 131
Msa1399470 AT4G03500.2 26.047 549 280 17 58 522 54 560 2.29e-30 126
Msa1399470 AT4G03500.3 26.047 549 280 17 58 522 54 560 2.29e-30 126
Msa1399470 AT4G03500.1 26.047 549 280 17 58 522 77 583 2.43e-30 126
Msa1399470 AT1G03670.1 25.581 516 328 9 74 562 107 593 2.56e-26 114
Msa1399470 AT4G03490.3 27.674 430 267 12 133 551 180 576 5.97e-25 110
Msa1399470 AT4G03490.5 27.830 424 262 12 133 545 170 560 7.94e-25 109
Msa1399470 AT4G03490.4 27.830 424 262 12 133 545 180 570 8.14e-25 109
Msa1399470 AT4G03460.2 26.406 409 256 8 128 522 158 535 8.35e-24 106
Msa1399470 AT4G03460.1 26.406 409 256 8 128 522 190 567 9.03e-24 106
Msa1399470 AT4G03490.2 24.915 586 332 18 65 574 9 562 1.03e-23 106
Msa1399470 AT5G50140.1 22.913 515 297 16 126 563 22 513 1.05e-23 105
Msa1399470 AT4G03490.1 25.277 542 318 15 65 551 19 528 2.42e-23 105
Msa1399470 AT5G54700.2 28.435 313 159 9 223 523 20 279 1.39e-22 99.8
Msa1399470 AT4G10720.1 24.096 415 258 12 169 559 40 421 3.32e-22 100
Msa1399470 AT5G54700.1 27.809 356 182 12 202 523 2 316 6.75e-22 99.8
Msa1399470 AT4G10720.2 25.076 327 210 6 169 477 40 349 1.96e-21 97.4
Msa1399470 AT4G14390.2 24.722 449 281 11 128 556 226 637 2.16e-20 95.9
Msa1399470 AT4G14390.4 24.722 449 281 11 128 556 245 656 2.63e-20 95.5
Msa1399470 AT4G14390.1 24.889 450 280 12 128 556 245 657 2.98e-20 95.5
Msa1399470 AT4G03480.1 26.076 395 232 9 118 482 295 659 1.57e-19 93.2
Msa1399470 AT4G03480.2 26.076 395 232 9 118 482 292 656 1.65e-19 93.2
Msa1399470 AT4G05040.3 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1399470 AT4G05040.2 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1399470 AT4G05040.5 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1399470 AT4G05040.4 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1399470 AT4G05040.1 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1399470 AT4G05040.6 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1399470 AT4G05040.7 28.736 348 199 9 118 447 211 527 9.01e-19 90.5
Msa1399470 AT5G15500.2 23.427 461 288 15 130 559 1 427 2.70e-18 88.2
Msa1399470 AT2G24600.2 26.420 405 232 14 72 450 70 434 1.75e-17 86.3
Msa1399470 AT2G24600.1 26.420 405 232 14 72 450 70 434 1.75e-17 86.3
Msa1399470 AT4G03450.2 24.505 555 306 17 58 528 45 570 1.81e-17 86.7
Msa1399470 AT5G51160.1 25.000 352 198 8 157 478 38 353 2.38e-17 85.5
Msa1399470 AT4G03450.1 24.505 555 306 17 58 528 45 570 5.28e-17 85.1
Msa1399470 AT4G03470.1 26.044 503 294 18 77 542 157 618 1.34e-16 84.0
Msa1399470 AT3G04140.1 27.622 286 160 8 100 342 105 386 2.92e-16 82.8
Msa1399470 AT1G14500.1 22.769 325 215 5 169 475 40 346 3.84e-15 78.6
Msa1399470 AT2G03430.1 34.659 176 106 5 135 306 16 186 8.50e-15 74.7
Msa1399470 AT4G14390.3 26.748 329 195 8 128 443 226 521 4.92e-13 72.4
Msa1399470 AT5G54620.1 29.570 186 119 2 169 342 40 225 8.08e-13 71.2
Msa1399470 AT5G54610.1 27.717 184 123 2 169 342 40 223 8.13e-13 71.2
Msa1399470 AT3G18670.1 23.111 450 296 12 123 530 113 554 1.09e-12 71.2
Msa1399470 AT2G28840.1 27.273 209 129 7 132 322 12 215 2.01e-12 70.1
Msa1399470 AT4G03440.1 23.126 467 308 12 104 550 258 693 3.95e-12 69.7
Msa1399470 AT4G03440.2 23.126 467 308 12 104 550 251 686 4.36e-12 69.7
Msa1399470 AT4G03440.3 23.126 467 308 12 104 550 251 686 4.36e-12 69.7
Msa1399470 AT4G14400.2 23.242 512 310 20 75 538 71 547 4.01e-11 66.2
Msa1399470 AT4G14400.3 23.242 512 310 20 75 538 71 547 4.01e-11 66.2
Msa1399470 AT3G18670.2 23.684 304 183 7 236 530 346 609 4.70e-11 66.2
Msa1399470 AT4G14400.1 23.242 512 310 20 75 538 137 613 5.68e-11 65.9

Find 168 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTGCATATGGCTGTAAA+AGG 0.222840 tig0022368:-6233 Msa1399470:CDS
TTATCCTTTCCAACGTAATT+TGG 0.223179 tig0022368:+4588 None:intergenic
TGATATTGATTCTCAGATAA+TGG 0.227356 tig0022368:-7486 Msa1399470:CDS
TAAGCTGCTGTTGTTGTTAT+AGG 0.245673 tig0022368:-4301 Msa1399470:intron
AATCATCATAATAATAATTT+AGG 0.249295 tig0022368:+7955 None:intergenic
GTGGAGTTTCCTCAGCTCTT+TGG 0.250605 tig0022368:+5163 None:intergenic
GTTATGCTTCCTGACAAATT+TGG 0.259995 tig0022368:-6161 Msa1399470:CDS
GAAAGGAAAGAGGGAGGAAA+TGG 0.261658 tig0022368:+8073 None:intergenic
GAACGAGTGGGCTGCAATTC+TGG 0.267500 tig0022368:-4805 Msa1399470:CDS
ACCTCCAATTTCAACCTTCC+AGG 0.283929 tig0022368:-7868 Msa1399470:intron
ACTCAAAGAGATTCAGAAAA+AGG 0.285730 tig0022368:-7731 Msa1399470:CDS
AAGGACATCCTCTCTCGATA+TGG 0.290107 tig0022368:-5314 Msa1399470:CDS
CCAACCTGGAAGGTTGAAAT+TGG 0.303178 tig0022368:+7864 None:intergenic
GTTATAGGATCATAACAAAT+TGG 0.307029 tig0022368:-4286 Msa1399470:three_prime_UTR
TTCATTTGCAGCCATTGTTC+AGG 0.330505 tig0022368:-6516 Msa1399470:intron
CTGCTTCTCTTTCTTCCTCA+TGG 0.331730 tig0022368:+4701 None:intergenic
GCAATTCTGGTTACAGTTGT+TGG 0.335512 tig0022368:-4792 Msa1399470:CDS
GATATTGATTCTCAGATAAT+GGG 0.350929 tig0022368:-7485 Msa1399470:CDS
AATTCGATCAACCTCGGAAT+TGG 0.351942 tig0022368:+4632 None:intergenic
AAAGGAAAGAGGGAGGAAAT+GGG 0.356439 tig0022368:+8074 None:intergenic
TGAAGAGTCATCAGATATAA+AGG 0.366092 tig0022368:-5333 Msa1399470:CDS
TAATAAGTGTTGGCTTTGAT+TGG 0.372963 tig0022368:+8014 None:intergenic
TTTGTTTACTGCTGCTGAAA+AGG 0.377535 tig0022368:-7366 Msa1399470:CDS
AGATGGCGACGGAGATATTT+CGG 0.380641 tig0022368:+7676 None:intergenic
GTTCAAATTACCCTTGTTAG+AGG 0.385400 tig0022368:-4942 Msa1399470:CDS
GTGATAATATCCGGAGTTAT+TGG 0.388823 tig0022368:-4765 Msa1399470:CDS
GTGAAACTAAAGCAGAGAAA+AGG 0.392404 tig0022368:-4920 Msa1399470:CDS
TTTGCATTTAGCTGCTCGTC+CGG 0.403120 tig0022368:-6333 Msa1399470:CDS
GTCAAAGGATGGCAGCTTGT+TGG 0.404723 tig0022368:-6384 Msa1399470:CDS
GGGTCATCTTGATGTTGTTA+AGG 0.408314 tig0022368:-7345 Msa1399470:CDS
AGAAAGAGAAGCAGCTAGCC+AGG 0.411228 tig0022368:-4692 Msa1399470:CDS
AGGACATCCTCTCTCGATAT+GGG 0.415929 tig0022368:-5313 Msa1399470:CDS
CACCACTGTCACCGAGTTTG+TGG 0.435710 tig0022368:+5121 None:intergenic
CTCGTCGCTGCTGTTATAAG+TGG 0.455896 tig0022368:+6437 None:intergenic
TCCGTCGTCCGCGACGGCGC+CGG 0.456322 tig0022368:-7654 Msa1399470:CDS
ATGGGAAAGTCGTGAGAAAG+AGG 0.462631 tig0022368:+8092 None:intergenic
AGGCTTTCACGATTTCTGTA+TGG 0.466846 tig0022368:+6309 None:intergenic
TTGCATTTAGCTGCTCGTCC+GGG 0.469773 tig0022368:-6332 Msa1399470:CDS
GCTGCTATATTCACCGTACC+TGG 0.472210 tig0022368:-5071 Msa1399470:CDS
TCCTCAGTTCGTCCCTTGGT+TGG 0.473921 tig0022368:+5267 None:intergenic
GAGTGGAAGGAAAGGAAAGA+GGG 0.474346 tig0022368:+8064 None:intergenic
CATTTACGTTTGTGTCTGGC+AGG 0.475438 tig0022368:+5411 None:intergenic
TTGTTTACTGCTGCTGAAAA+GGG 0.479987 tig0022368:-7365 Msa1399470:CDS
GCTCAGCAAAGACCCTCAAT+TGG 0.481998 tig0022368:-6285 Msa1399470:CDS
CTTTAGTTTCACCTCTAACA+AGG 0.482809 tig0022368:+4931 None:intergenic
CCAAGCTTAAGCAAAACTAT+TGG 0.486154 tig0022368:-6476 Msa1399470:CDS
GAAAAGATGAGTGGAAGGAA+AGG 0.490183 tig0022368:+8056 None:intergenic
TGAGTTGGATCCACTTCTCC+TGG 0.491866 tig0022368:+4674 None:intergenic
GGGGATAACGATGATGGATC+AGG 0.492044 tig0022368:-5047 Msa1399470:CDS
CAGTCCTCCGAGCCAATTGA+GGG 0.492228 tig0022368:+6273 None:intergenic
TAATAATAATTTAGGAAGGA+AGG 0.493048 tig0022368:+7963 None:intergenic
TCCTTTCAAACTCAGGAAAA+CGG 0.494021 tig0022368:-7625 Msa1399470:CDS
TGAGTGGAAGGAAAGGAAAG+AGG 0.495388 tig0022368:+8063 None:intergenic
TGAAACTAAAGCAGAGAAAA+GGG 0.501105 tig0022368:-4919 Msa1399470:CDS
AGGGCAAACTGCATTGCATA+TGG 0.501695 tig0022368:-6243 Msa1399470:CDS
TCAGCTCTTTGGATATATTG+TGG 0.502735 tig0022368:+5174 None:intergenic
GGTCATGATAGAAGAGTAGA+GGG 0.503098 tig0022368:+7888 None:intergenic
ATCGTTATCCCCACCAGGTA+CGG 0.503271 tig0022368:+5058 None:intergenic
TTGAGGGTCTTTGCTGAGCA+AGG 0.503619 tig0022368:+6289 None:intergenic
AATGCATTTACGTTTGTGTC+TGG 0.503703 tig0022368:+5407 None:intergenic
TTGGAAATTGCAAGATCCAA+TGG 0.508338 tig0022368:-6365 Msa1399470:CDS
AAGTACGTGAAGCAAGTAAC+GGG 0.508570 tig0022368:-7581 Msa1399470:CDS
GCTCGGAGGACTGACAAGAA+AGG 0.509842 tig0022368:-6263 Msa1399470:CDS
AAAGAGAAAAGATGAGTGGA+AGG 0.510130 tig0022368:+8051 None:intergenic
GACGGAGATATTTCGGAAAG+TGG 0.514348 tig0022368:+7683 None:intergenic
CTCGGAGGACTGACAAGAAA+GGG 0.519611 tig0022368:-6262 Msa1399470:CDS
TTCACGATTTCTGTATGGCC+CGG 0.525638 tig0022368:+6314 None:intergenic
TATGCATTTGTATAAATCAC+AGG 0.525663 tig0022368:-4205 Msa1399470:three_prime_UTR
GTTGGAGGAGTGATAATATC+CGG 0.527167 tig0022368:-4774 Msa1399470:CDS
GCCGGCGCCGTCGCGGACGA+CGG 0.529146 tig0022368:+7653 None:intergenic
CATTGCATATGGCTGTAAAA+GGG 0.531587 tig0022368:-6232 Msa1399470:CDS
AAGACCAAATTACGTTGGAA+AGG 0.532286 tig0022368:-4592 Msa1399470:three_prime_UTR
GAAAGATGTTCACACACAGC+TGG 0.535788 tig0022368:-5225 Msa1399470:CDS
CCTGGTGGGGATAACGATGA+TGG 0.535860 tig0022368:-5053 Msa1399470:CDS
GCCATCTCCGTCGTCCGCGA+CGG 0.536247 tig0022368:-7660 Msa1399470:CDS
GCTGTGTTGCCAAATTTGTC+AGG 0.537540 tig0022368:+6152 None:intergenic
GGAAAACGGATCGATCAAGC+AGG 0.537963 tig0022368:-7611 Msa1399470:CDS
CTATATTCACCGTACCTGGT+GGG 0.538227 tig0022368:-5067 Msa1399470:CDS
CAGCATTGCATGTGGCAACC+AGG 0.541967 tig0022368:-6133 Msa1399470:CDS
AAGCAGCTAGCCAGGAGAAG+TGG 0.542236 tig0022368:-4684 Msa1399470:CDS
TCAGTCCTCCGAGCCAATTG+AGG 0.545108 tig0022368:+6272 None:intergenic
TCGGAAAGTGGGTTCTGTGT+TGG 0.547229 tig0022368:+7695 None:intergenic
GACTAGAGCCGGCGCCGTCG+CGG 0.548417 tig0022368:+7646 None:intergenic
GAAAGTCGTGAGAAAGAGGG+AGG 0.548432 tig0022368:+8096 None:intergenic
TTGCATATTGCTGCTAGTCA+AGG 0.549825 tig0022368:-7260 Msa1399470:CDS
CAGATAATGGGTACTTTGAG+TGG 0.549949 tig0022368:-7473 Msa1399470:CDS
TGGAAGGAAAGGAAAGAGGG+AGG 0.550810 tig0022368:+8067 None:intergenic
AAGTCGTGAGAAAGAGGGAG+GGG 0.550978 tig0022368:+8098 None:intergenic
TGGGAAAGTCGTGAGAAAGA+GGG 0.551251 tig0022368:+8093 None:intergenic
CTCTGAATGATGCCATGAGT+TGG 0.551504 tig0022368:+4659 None:intergenic
AAGAGCTGAGGAAACTCCAC+AGG 0.552962 tig0022368:-5160 Msa1399470:CDS
ATCTATGTAAACTCTTGAAG+TGG 0.553547 tig0022368:+4446 None:intergenic
ATCTATGTAAACTCTTGAAG+TGG 0.553547 tig0022368:+4531 None:intergenic
ATTCTGGTTACAGTTGTTGG+AGG 0.554005 tig0022368:-4789 Msa1399470:CDS
GATTAACAAGCTCATGTGGT+TGG 0.554304 tig0022368:-4886 Msa1399470:CDS
AAGGGACGAACTGAGGAAAA+CGG 0.554349 tig0022368:-5261 Msa1399470:CDS
AGGTCATGATAGAAGAGTAG+AGG 0.556929 tig0022368:+7887 None:intergenic
AAAGTCGTGAGAAAGAGGGA+GGG 0.557510 tig0022368:+8097 None:intergenic
ACCTGGAAGGTTGAAATTGG+AGG 0.558297 tig0022368:+7867 None:intergenic
GCCGTCGCGGACGACGGAGA+TGG 0.559929 tig0022368:+7659 None:intergenic
CCATCATCGTTATCCCCACC+AGG 0.564948 tig0022368:+5053 None:intergenic
TTTAGTTTCACCTCTAACAA+GGG 0.565107 tig0022368:+4932 None:intergenic
GTTTGAAGACCAAATTACGT+TGG 0.566835 tig0022368:-4597 Msa1399470:three_prime_UTR
GAGTTTGAAAGGACTAGAGC+CGG 0.567456 tig0022368:+7635 None:intergenic
GCAAAGACCCTCAATTGGCT+CGG 0.569896 tig0022368:-6280 Msa1399470:CDS
GCTCTAGTCCTTTCAAACTC+AGG 0.572097 tig0022368:-7632 Msa1399470:CDS
CATTGTTAATTCCTTCCCTG+TGG 0.572226 tig0022368:+5144 None:intergenic
AGCAGAGAAAAGGGTAGTTG+AGG 0.573019 tig0022368:-4910 Msa1399470:CDS
TCGTCGGTCGGAGGAACGAG+TGG 0.574175 tig0022368:-4818 Msa1399470:CDS
CGCTGCTGTTATAAGTGGAG+TGG 0.575162 tig0022368:+6442 None:intergenic
GCTGCTAGTCAAGGACATCA+TGG 0.575482 tig0022368:-7251 Msa1399470:intron
ATAAGTGGAGTGGCGTTGGA+TGG 0.578501 tig0022368:+6452 None:intergenic
GGAAGGAAGGATGAAAGTGA+AGG 0.582860 tig0022368:+7976 None:intergenic
GCAGCTCAAAGAGGTGATGT+TGG 0.583391 tig0022368:-7530 Msa1399470:CDS
GTGAATGAAGAGAATGAACT+TGG 0.587406 tig0022368:-7398 Msa1399470:CDS
TCTGAGAATCAATATCAAGA+AGG 0.587872 tig0022368:+7492 None:intergenic
ACGGAGATATTTCGGAAAGT+GGG 0.587917 tig0022368:+7684 None:intergenic
TGTTATAAGTGGAGTGGCGT+TGG 0.592828 tig0022368:+6448 None:intergenic
TTCAGAGTTCTCCAATTCCG+AGG 0.592884 tig0022368:-4643 Msa1399470:CDS
GCATGAGTCATAATAAGTGT+TGG 0.594195 tig0022368:+8004 None:intergenic
ATCATAATAATAATTTAGGA+AGG 0.594731 tig0022368:+7959 None:intergenic
GCTATATTCACCGTACCTGG+TGG 0.598478 tig0022368:-5068 Msa1399470:CDS
ATCTAGTAAAACCTGAACAA+TGG 0.600510 tig0022368:+6505 None:intergenic
GCCTCTTCGTACATAGTCGT+CGG 0.600824 tig0022368:-4834 Msa1399470:CDS
CGCATAAATTCGATCAACCT+CGG 0.601587 tig0022368:+4626 None:intergenic
TGATAAAGAGAAAAGATGAG+TGG 0.602428 tig0022368:+8047 None:intergenic
CGTACATAGTCGTCGGTCGG+AGG 0.602499 tig0022368:-4827 Msa1399470:CDS
GTTGGCTTCTGTCTGTACCT+CGG 0.602568 tig0022368:-4868 Msa1399470:CDS
CTGAGGAAACTCCACAGGGA+AGG 0.603249 tig0022368:-5155 Msa1399470:CDS
TATATTCACCGTACCTGGTG+GGG 0.603908 tig0022368:-5066 Msa1399470:CDS
GAAGTACGTGAAGCAAGTAA+CGG 0.604371 tig0022368:-7582 Msa1399470:CDS
GGAGAAGTGGATCCAACTCA+TGG 0.605497 tig0022368:-4671 Msa1399470:CDS
AGAAGAAGAACCAACCTGGA+AGG 0.609441 tig0022368:+7854 None:intergenic
GACGAGAGGGCACGTCGAAG+TGG 0.609993 tig0022368:-6420 Msa1399470:CDS
GGTGAATGAACTTCTGTCAA+AGG 0.612992 tig0022368:-6399 Msa1399470:CDS
TTATAACAGCAGCGACGAGA+GGG 0.613377 tig0022368:-6433 Msa1399470:CDS
AATGAACTTCTGTCAAAGGA+TGG 0.613527 tig0022368:-6395 Msa1399470:CDS
TCTGATGACTCTTCAGAGAG+AGG 0.615975 tig0022368:+5341 None:intergenic
AATTAACAATGCCACAAACT+CGG 0.616650 tig0022368:-5132 Msa1399470:CDS
CGTCGGTCGGAGGAACGAGT+GGG 0.619749 tig0022368:-4817 Msa1399470:CDS
ACCAACCAAGGGACGAACTG+AGG 0.620407 tig0022368:-5268 Msa1399470:CDS
CTAACGAGCTCAACCAACCA+AGG 0.621035 tig0022368:-5280 Msa1399470:CDS
CTTCATCTAGCAGCTCAAAG+AGG 0.623399 tig0022368:-7539 Msa1399470:CDS
CTTCGTACATAGTCGTCGGT+CGG 0.624785 tig0022368:-4830 Msa1399470:CDS
CTTATAACAGCAGCGACGAG+AGG 0.631608 tig0022368:-6434 Msa1399470:CDS
TGCCACAAACTCGGTGACAG+TGG 0.632510 tig0022368:-5123 Msa1399470:CDS
AGTCATAGTGCCAATAACTC+CGG 0.636725 tig0022368:+4755 None:intergenic
TGGCAACACAGCATTGCATG+TGG 0.637663 tig0022368:-6141 Msa1399470:CDS
GGACATCCTCTCTCGATATG+GGG 0.638757 tig0022368:-5312 Msa1399470:CDS
TGACTAGCAGCAATATGCAA+AGG 0.639675 tig0022368:+7263 None:intergenic
ACAATATATCCAAAGAGCTG+AGG 0.644642 tig0022368:-5172 Msa1399470:CDS
AAGACCCTCAATTGGCTCGG+AGG 0.651684 tig0022368:-6277 Msa1399470:CDS
GCGGACGACGGAGATGGCGA+CGG 0.652416 tig0022368:+7665 None:intergenic
GGCTTCTGTCTGTACCTCGG+TGG 0.655940 tig0022368:-4865 Msa1399470:CDS
GAAAACGGATCGATCAAGCA+GGG 0.663000 tig0022368:-7610 Msa1399470:CDS
TAACGATGATGGATCAGGAG+TGG 0.663853 tig0022368:-5042 Msa1399470:CDS
TAAGTGCCCCATATCGAGAG+AGG 0.665007 tig0022368:+5306 None:intergenic
AGGTGATTAACAAGCTCATG+TGG 0.671742 tig0022368:-4890 Msa1399470:CDS
ACCGACGACTATGTACGAAG+AGG 0.681066 tig0022368:+4833 None:intergenic
CACAAACTCGGTGACAGTGG+TGG 0.691648 tig0022368:-5120 Msa1399470:CDS
TAACGAGCTCAACCAACCAA+GGG 0.693605 tig0022368:-5279 Msa1399470:CDS
CTATGACTTACTATGTAGTG+AGG 0.708286 tig0022368:-4740 Msa1399470:CDS
CTAAGAGAACAAGATCCATG+AGG 0.720939 tig0022368:-4716 Msa1399470:CDS
AGAGCTGAGGAAACTCCACA+GGG 0.730372 tig0022368:-5159 Msa1399470:CDS
AAAAGGGCAGAGCTGTGACG+TGG 0.742175 tig0022368:-6216 Msa1399470:CDS
GTCATGATAGAAGAGTAGAG+GGG 0.743790 tig0022368:+7889 None:intergenic
AGAGGCAATAAATGCCACCG+AGG 0.784742 tig0022368:+4851 None:intergenic
GATTTGTGAGAAGAATAACA+CGG 0.801731 tig0022368:-4178 Msa1399470:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATCATAATAATAATTT+AGG + tig0022368:4281-4300 None:intergenic 10.0%
!! ATCATAATAATAATTTAGGA+AGG + tig0022368:4277-4296 None:intergenic 15.0%
!!! TATTTTGCTTTTTGTAAAAA+TGG - tig0022368:5094-5113 Msa1399470:CDS 15.0%
!!! ATTTTGCTTTTTGTAAAAAT+GGG - tig0022368:5095-5114 Msa1399470:CDS 15.0%
!!! TTTTTTTAAGAAGCTATTAA+TGG - tig0022368:5486-5505 Msa1399470:intron 15.0%
!! AAAAATTAAAAGAAAACAAG+AGG + tig0022368:6685-6704 None:intergenic 15.0%
!!! AATATTAGATTTTTCATTCA+AGG + tig0022368:6758-6777 None:intergenic 15.0%
!! TAATAATAATTTAGGAAGGA+AGG + tig0022368:4273-4292 None:intergenic 20.0%
!!! TATTTTGAATGAATTGACTT+TGG - tig0022368:5129-5148 Msa1399470:CDS 20.0%
!!! TTTTTTTTGGAACATATTCT+TGG - tig0022368:5327-5346 Msa1399470:CDS 20.0%
!! AATTTAATCAGATATACTTC+TGG + tig0022368:5375-5394 None:intergenic 20.0%
!! TCATAATTGAGTTTCTTTAT+TGG + tig0022368:5452-5471 None:intergenic 20.0%
!! TAATACTACTACTACAAATA+TGG + tig0022368:6259-6278 None:intergenic 20.0%
!! GCAACTACTTAAATAAATAA+AGG + tig0022368:6487-6506 None:intergenic 20.0%
!!! TGTTATTTTGCTTTGAAAAT+TGG - tig0022368:6561-6580 Msa1399470:intron 20.0%
! TTACAGTGAAGTAAAAAGAT+GGG + tig0022368:4414-4433 None:intergenic 25.0%
! TTTACAGTGAAGTAAAAAGA+TGG + tig0022368:4415-4434 None:intergenic 25.0%
!!! TGATATTGATTCTCAGATAA+TGG - tig0022368:4747-4766 Msa1399470:CDS 25.0%
!!! GATATTGATTCTCAGATAAT+GGG - tig0022368:4748-4767 Msa1399470:CDS 25.0%
! AACATCAAAACAAAGGAAAA+AGG + tig0022368:5055-5074 None:intergenic 25.0%
!!! TTTTTCCTTTGTTTTGATGT+TGG - tig0022368:5054-5073 Msa1399470:CDS 25.0%
!!! TTTTCCTTTGTTTTGATGTT+GGG - tig0022368:5055-5074 Msa1399470:CDS 25.0%
! AAGGTAAAACGATTTATGTT+TGG - tig0022368:5166-5185 Msa1399470:CDS 25.0%
! AGGTAAAACGATTTATGTTT+GGG - tig0022368:5167-5186 Msa1399470:CDS 25.0%
! TTGAGTATTTATCTTGCTTA+AGG - tig0022368:5423-5442 Msa1399470:CDS 25.0%
! CTCAATTATGATGCTATTAA+TGG - tig0022368:5461-5480 Msa1399470:intron 25.0%
! ATTAACTTACTCGAACAAAT+CGG - tig0022368:6209-6228 Msa1399470:CDS 25.0%
! TTTAAGTAGTTGCAAGTATA+AGG - tig0022368:6494-6513 Msa1399470:CDS 25.0%
! AAACATGTTATATGAGACAA+GGG - tig0022368:6627-6646 Msa1399470:intron 25.0%
!! TCAATTTTGTCTAGAAGTTT+TGG - tig0022368:6658-6677 Msa1399470:intron 25.0%
!! CAATTTTGTCTAGAAGTTTT+GGG - tig0022368:6659-6678 Msa1399470:intron 25.0%
!!! ATTGTTGTTTCTCAATTTCT+TGG - tig0022368:6775-6794 Msa1399470:intron 25.0%
!! TATATTGTGGACATTTTTGT+TGG + tig0022368:7049-7068 None:intergenic 25.0%
! GTTATAGGATCATAACAAAT+TGG - tig0022368:7947-7966 Msa1399470:five_prime_UTR 25.0%
! TATGCATTTGTATAAATCAC+AGG - tig0022368:8028-8047 Msa1399470:five_prime_UTR 25.0%
TGATAAAGAGAAAAGATGAG+TGG + tig0022368:4189-4208 None:intergenic 30.0%
!! TAATAAGTGTTGGCTTTGAT+TGG + tig0022368:4222-4241 None:intergenic 30.0%
TACAGTGAAGTAAAAAGATG+GGG + tig0022368:4413-4432 None:intergenic 30.0%
ACTCAAAGAGATTCAGAAAA+AGG - tig0022368:4502-4521 Msa1399470:three_prime_UTR 30.0%
TCTGAGAATCAATATCAAGA+AGG + tig0022368:4744-4763 None:intergenic 30.0%
! GTTTCTAAAAAGAATCGATC+TGG - tig0022368:4940-4959 Msa1399470:CDS 30.0%
AAATCCCAACATCAAAACAA+AGG + tig0022368:5062-5081 None:intergenic 30.0%
!!! TTGTTTTGATGTTGGGATTT+TGG - tig0022368:5062-5081 Msa1399470:CDS 30.0%
!! TTTGGCTAAAATTGAGTTGA+AGG - tig0022368:5147-5166 Msa1399470:CDS 30.0%
ACATAGCATTAACTCTACTA+CGG + tig0022368:5557-5576 None:intergenic 30.0%
AATAAGACAACGTTGATGTT+TGG + tig0022368:5591-5610 None:intergenic 30.0%
! AACATCAACGTTGTCTTATT+TGG - tig0022368:5591-5610 Msa1399470:intron 30.0%
AGATTACTACAAAGAACAGA+AGG + tig0022368:5687-5706 None:intergenic 30.0%
ATCTAGTAAAACCTGAACAA+TGG + tig0022368:5731-5750 None:intergenic 30.0%
!! CAATAGTTTTGCTTAAGCTT+GGG + tig0022368:5759-5778 None:intergenic 30.0%
!!! ATGCAAAGCATTTTTTCCAT+TGG + tig0022368:5887-5906 None:intergenic 30.0%
TCATCACTATATGTACTGTA+TGG + tig0022368:6179-6198 None:intergenic 30.0%
TTAACTTACTCGAACAAATC+GGG - tig0022368:6210-6229 Msa1399470:CDS 30.0%
AAGTCTCCAATATTGAAAAC+AGG - tig0022368:6296-6315 Msa1399470:CDS 30.0%
! TAGCTACCTGTTTTCAATAT+TGG + tig0022368:6305-6324 None:intergenic 30.0%
GTGCACACTACTTAAAAAAA+TGG + tig0022368:6597-6616 None:intergenic 30.0%
GAAACATGTTATATGAGACA+AGG - tig0022368:6626-6645 Msa1399470:intron 30.0%
! AAGATTTTCAGCAATGTCAA+GGG + tig0022368:6872-6891 None:intergenic 30.0%
TGAAGAGTCATCAGATATAA+AGG - tig0022368:6900-6919 Msa1399470:intron 30.0%
AATTAACAATGCCACAAACT+CGG - tig0022368:7101-7120 Msa1399470:intron 30.0%
!! TATTGCGCTTTTTACATCTT+TGG - tig0022368:7257-7276 Msa1399470:CDS 30.0%
! TTTAGTTTCACCTCTAACAA+GGG + tig0022368:7304-7323 None:intergenic 30.0%
TGAAACTAAAGCAGAGAAAA+GGG - tig0022368:7314-7333 Msa1399470:CDS 30.0%
TTATCCTTTCCAACGTAATT+TGG + tig0022368:7648-7667 None:intergenic 30.0%
ATCTATGTAAACTCTTGAAG+TGG + tig0022368:7705-7724 None:intergenic 30.0%
ATCTATGTAAACTCTTGAAG+TGG + tig0022368:7705-7724 None:intergenic 30.0%
GATTTGTGAGAAGAATAACA+CGG - tig0022368:8055-8074 Msa1399470:five_prime_UTR 30.0%
AAAGAGAAAAGATGAGTGGA+AGG + tig0022368:4185-4204 None:intergenic 35.0%
GCATGAGTCATAATAAGTGT+TGG + tig0022368:4232-4251 None:intergenic 35.0%
ACAGTGAAGTAAAAAGATGG+GGG + tig0022368:4412-4431 None:intergenic 35.0%
! TACGCTTTTACGTTTATTGC+AGG - tig0022368:4469-4488 Msa1399470:three_prime_UTR 35.0%
TCCTTTCAAACTCAGGAAAA+CGG - tig0022368:4608-4627 Msa1399470:exon 35.0%
GTGAATGAAGAGAATGAACT+TGG - tig0022368:4835-4854 Msa1399470:CDS 35.0%
TTTGTTTACTGCTGCTGAAA+AGG - tig0022368:4867-4886 Msa1399470:CDS 35.0%
TTGTTTACTGCTGCTGAAAA+GGG - tig0022368:4868-4887 Msa1399470:CDS 35.0%
GCTTCAAGTAGAATCAGTTA+TGG - tig0022368:5265-5284 Msa1399470:CDS 35.0%
TCAGTTATGGAGACAAAATC+AGG - tig0022368:5278-5297 Msa1399470:CDS 35.0%
CTTGAGTAGCTTCAATTTGT+GGG - tig0022368:5630-5649 Msa1399470:intron 35.0%
! TAGCTTCAATTTGTGGGTTA+TGG - tig0022368:5636-5655 Msa1399470:intron 35.0%
!! CCAATAGTTTTGCTTAAGCT+TGG + tig0022368:5760-5779 None:intergenic 35.0%
CCAAGCTTAAGCAAAACTAT+TGG - tig0022368:5757-5776 Msa1399470:intron 35.0%
AATGAACTTCTGTCAAAGGA+TGG - tig0022368:5838-5857 Msa1399470:intron 35.0%
TTGGAAATTGCAAGATCCAA+TGG - tig0022368:5868-5887 Msa1399470:intron 35.0%
CATTGCATATGGCTGTAAAA+GGG - tig0022368:6001-6020 Msa1399470:intron 35.0%
GTTATGCTTCCTGACAAATT+TGG - tig0022368:6072-6091 Msa1399470:intron 35.0%
TGCTACAAAAAATGCAAACG+AGG - tig0022368:6329-6348 Msa1399470:CDS 35.0%
AAGTAGTTGCAAGTATAAGG+TGG - tig0022368:6497-6516 Msa1399470:CDS 35.0%
ACATGCTCAAAAGTAACTAG+GGG + tig0022368:6728-6747 None:intergenic 35.0%
GACATGCTCAAAAGTAACTA+GGG + tig0022368:6729-6748 None:intergenic 35.0%
TGACATGCTCAAAAGTAACT+AGG + tig0022368:6730-6749 None:intergenic 35.0%
AATGCATTTACGTTTGTGTC+TGG + tig0022368:6829-6848 None:intergenic 35.0%
! GAAGATTTTCAGCAATGTCA+AGG + tig0022368:6873-6892 None:intergenic 35.0%
!!! GACATTTTTGTTGGTTCTCT+TGG + tig0022368:7040-7059 None:intergenic 35.0%
TCAGCTCTTTGGATATATTG+TGG + tig0022368:7062-7081 None:intergenic 35.0%
ACAATATATCCAAAGAGCTG+AGG - tig0022368:7061-7080 Msa1399470:intron 35.0%
! TTTTACATCTTTGGCTGTTG+TGG - tig0022368:7266-7285 Msa1399470:CDS 35.0%
GTTCAAATTACCCTTGTTAG+AGG - tig0022368:7291-7310 Msa1399470:CDS 35.0%
! CTTTAGTTTCACCTCTAACA+AGG + tig0022368:7305-7324 None:intergenic 35.0%
GTGAAACTAAAGCAGAGAAA+AGG - tig0022368:7313-7332 Msa1399470:CDS 35.0%
GTGATAATATCCGGAGTTAT+TGG - tig0022368:7468-7487 Msa1399470:CDS 35.0%
CTATGACTTACTATGTAGTG+AGG - tig0022368:7493-7512 Msa1399470:CDS 35.0%
GTTTGAAGACCAAATTACGT+TGG - tig0022368:7636-7655 Msa1399470:CDS 35.0%
AAGACCAAATTACGTTGGAA+AGG - tig0022368:7641-7660 Msa1399470:CDS 35.0%
! TAAGCTGCTGTTGTTGTTAT+AGG - tig0022368:7932-7951 Msa1399470:five_prime_UTR 35.0%
AAAGGAAAGAGGGAGGAAAT+GGG + tig0022368:4162-4181 None:intergenic 40.0%
GAAAAGATGAGTGGAAGGAA+AGG + tig0022368:4180-4199 None:intergenic 40.0%
GTCATGATAGAAGAGTAGAG+GGG + tig0022368:4347-4366 None:intergenic 40.0%
GGTCATGATAGAAGAGTAGA+GGG + tig0022368:4348-4367 None:intergenic 40.0%
AGGTCATGATAGAAGAGTAG+AGG + tig0022368:4349-4368 None:intergenic 40.0%
AGATAGAAGAAGAACCAACC+TGG + tig0022368:4386-4405 None:intergenic 40.0%
! GCTTTTACGTTTATTGCAGG+TGG - tig0022368:4472-4491 Msa1399470:three_prime_UTR 40.0%
ACGGAGATATTTCGGAAAGT+GGG + tig0022368:4552-4571 None:intergenic 40.0%
! TCCGTTTTCCTGAGTTTGAA+AGG + tig0022368:4612-4631 None:intergenic 40.0%
GAAGTACGTGAAGCAAGTAA+CGG - tig0022368:4651-4670 Msa1399470:CDS 40.0%
AAGTACGTGAAGCAAGTAAC+GGG - tig0022368:4652-4671 Msa1399470:CDS 40.0%
!! CAGATAATGGGTACTTTGAG+TGG - tig0022368:4760-4779 Msa1399470:CDS 40.0%
GGGTCATCTTGATGTTGTTA+AGG - tig0022368:4888-4907 Msa1399470:CDS 40.0%
TGACTAGCAGCAATATGCAA+AGG + tig0022368:4973-4992 None:intergenic 40.0%
TTGCATATTGCTGCTAGTCA+AGG - tig0022368:4973-4992 Msa1399470:CDS 40.0%
GCTACTCAAGGTCTAACAAT+TGG + tig0022368:5620-5639 None:intergenic 40.0%
CCACAAATTGAAGCTACTCA+AGG + tig0022368:5632-5651 None:intergenic 40.0%
CCTTGAGTAGCTTCAATTTG+TGG - tig0022368:5629-5648 Msa1399470:intron 40.0%
TTCATTTGCAGCCATTGTTC+AGG - tig0022368:5717-5736 Msa1399470:intron 40.0%
GGTGAATGAACTTCTGTCAA+AGG - tig0022368:5834-5853 Msa1399470:intron 40.0%
AGGCTTTCACGATTTCTGTA+TGG + tig0022368:5927-5946 None:intergenic 40.0%
GCATTGCATATGGCTGTAAA+AGG - tig0022368:6000-6019 Msa1399470:intron 40.0%
CATTGTTAATTCCTTCCCTG+TGG + tig0022368:7092-7111 None:intergenic 40.0%
AGGTGATTAACAAGCTCATG+TGG - tig0022368:7343-7362 Msa1399470:CDS 40.0%
GATTAACAAGCTCATGTGGT+TGG - tig0022368:7347-7366 Msa1399470:CDS 40.0%
! GCAATTCTGGTTACAGTTGT+TGG - tig0022368:7441-7460 Msa1399470:CDS 40.0%
! ATTCTGGTTACAGTTGTTGG+AGG - tig0022368:7444-7463 Msa1399470:CDS 40.0%
GTTGGAGGAGTGATAATATC+CGG - tig0022368:7459-7478 Msa1399470:CDS 40.0%
AGTCATAGTGCCAATAACTC+CGG + tig0022368:7481-7500 None:intergenic 40.0%
CTAAGAGAACAAGATCCATG+AGG - tig0022368:7517-7536 Msa1399470:CDS 40.0%
AATTCGATCAACCTCGGAAT+TGG + tig0022368:7604-7623 None:intergenic 40.0%
CGCATAAATTCGATCAACCT+CGG + tig0022368:7610-7629 None:intergenic 40.0%
AAAGTCGTGAGAAAGAGGGA+GGG + tig0022368:4139-4158 None:intergenic 45.0%
TGGGAAAGTCGTGAGAAAGA+GGG + tig0022368:4143-4162 None:intergenic 45.0%
ATGGGAAAGTCGTGAGAAAG+AGG + tig0022368:4144-4163 None:intergenic 45.0%
GAAAGGAAAGAGGGAGGAAA+TGG + tig0022368:4163-4182 None:intergenic 45.0%
GAGTGGAAGGAAAGGAAAGA+GGG + tig0022368:4172-4191 None:intergenic 45.0%
TGAGTGGAAGGAAAGGAAAG+AGG + tig0022368:4173-4192 None:intergenic 45.0%
GGAAGGAAGGATGAAAGTGA+AGG + tig0022368:4260-4279 None:intergenic 45.0%
ACCTCCAATTTCAACCTTCC+AGG - tig0022368:4365-4384 Msa1399470:intron 45.0%
ACCTGGAAGGTTGAAATTGG+AGG + tig0022368:4369-4388 None:intergenic 45.0%
CCAACCTGGAAGGTTGAAAT+TGG + tig0022368:4372-4391 None:intergenic 45.0%
CCAATTTCAACCTTCCAGGT+TGG - tig0022368:4369-4388 Msa1399470:intron 45.0%
AGAAGAAGAACCAACCTGGA+AGG + tig0022368:4382-4401 None:intergenic 45.0%
GACGGAGATATTTCGGAAAG+TGG + tig0022368:4553-4572 None:intergenic 45.0%
AGATGGCGACGGAGATATTT+CGG + tig0022368:4560-4579 None:intergenic 45.0%
GAGTTTGAAAGGACTAGAGC+CGG + tig0022368:4601-4620 None:intergenic 45.0%
GCTCTAGTCCTTTCAAACTC+AGG - tig0022368:4601-4620 Msa1399470:exon 45.0%
GAAAACGGATCGATCAAGCA+GGG - tig0022368:4623-4642 Msa1399470:CDS 45.0%
CTTCATCTAGCAGCTCAAAG+AGG - tig0022368:4694-4713 Msa1399470:CDS 45.0%
TGTTATAAGTGGAGTGGCGT+TGG + tig0022368:5788-5807 None:intergenic 45.0%
TTATAACAGCAGCGACGAGA+GGG - tig0022368:5800-5819 Msa1399470:intron 45.0%
TTTGCATTTAGCTGCTCGTC+CGG - tig0022368:5900-5919 Msa1399470:intron 45.0%
TTCACGATTTCTGTATGGCC+CGG + tig0022368:5922-5941 None:intergenic 45.0%
AGGGCAAACTGCATTGCATA+TGG - tig0022368:5990-6009 Msa1399470:intron 45.0%
GCTGTGTTGCCAAATTTGTC+AGG + tig0022368:6084-6103 None:intergenic 45.0%
AACCAGGAAAAAGCGAGTAG+AGG - tig0022368:6116-6135 Msa1399470:intron 45.0%
!!! TACCTCTACTCGCTTTTTCC+TGG + tig0022368:6121-6140 None:intergenic 45.0%
CATTTACGTTTGTGTCTGGC+AGG + tig0022368:6825-6844 None:intergenic 45.0%
!! CAATGTCAAGGGCTGTTTTG+TGG + tig0022368:6861-6880 None:intergenic 45.0%
TCTGATGACTCTTCAGAGAG+AGG + tig0022368:6895-6914 None:intergenic 45.0%
AAGGACATCCTCTCTCGATA+TGG - tig0022368:6919-6938 Msa1399470:intron 45.0%
AGGACATCCTCTCTCGATAT+GGG - tig0022368:6920-6939 Msa1399470:intron 45.0%
TAACGAGCTCAACCAACCAA+GGG - tig0022368:6954-6973 Msa1399470:intron 45.0%
AAGGGACGAACTGAGGAAAA+CGG - tig0022368:6972-6991 Msa1399470:intron 45.0%
GAAAGATGTTCACACACAGC+TGG - tig0022368:7008-7027 Msa1399470:intron 45.0%
CTATATTCACCGTACCTGGT+GGG - tig0022368:7166-7185 Msa1399470:intron 45.0%
TATATTCACCGTACCTGGTG+GGG - tig0022368:7167-7186 Msa1399470:intron 45.0%
!! TAACGATGATGGATCAGGAG+TGG - tig0022368:7191-7210 Msa1399470:intron 45.0%
AGCAGAGAAAAGGGTAGTTG+AGG - tig0022368:7323-7342 Msa1399470:CDS 45.0%
CTGCTTCTCTTTCTTCCTCA+TGG + tig0022368:7535-7554 None:intergenic 45.0%
CTCTGAATGATGCCATGAGT+TGG + tig0022368:7577-7596 None:intergenic 45.0%
TTCAGAGTTCTCCAATTCCG+AGG - tig0022368:7590-7609 Msa1399470:CDS 45.0%
!! GAGTTTTGTGCACTTCAGCA+TGG - tig0022368:7999-8018 Msa1399470:five_prime_UTR 45.0%
!!! TTTTGTATTTTTTTTTTTTT+TGG - tig0022368:5314-5333 Msa1399470:CDS 5.0%
AAGTCGTGAGAAAGAGGGAG+GGG + tig0022368:4138-4157 None:intergenic 50.0%
GAAAGTCGTGAGAAAGAGGG+AGG + tig0022368:4140-4159 None:intergenic 50.0%
TGGAAGGAAAGGAAAGAGGG+AGG + tig0022368:4169-4188 None:intergenic 50.0%
! TCGGAAAGTGGGTTCTGTGT+TGG + tig0022368:4541-4560 None:intergenic 50.0%
GGAAAACGGATCGATCAAGC+AGG - tig0022368:4622-4641 Msa1399470:CDS 50.0%
GCAGCTCAAAGAGGTGATGT+TGG - tig0022368:4703-4722 Msa1399470:CDS 50.0%
GCTGCTAGTCAAGGACATCA+TGG - tig0022368:4982-5001 Msa1399470:CDS 50.0%
! ATAAGTGGAGTGGCGTTGGA+TGG + tig0022368:5784-5803 None:intergenic 50.0%
CGCTGCTGTTATAAGTGGAG+TGG + tig0022368:5794-5813 None:intergenic 50.0%
CTCGTCGCTGCTGTTATAAG+TGG + tig0022368:5799-5818 None:intergenic 50.0%
CTTATAACAGCAGCGACGAG+AGG - tig0022368:5799-5818 Msa1399470:intron 50.0%
GTCAAAGGATGGCAGCTTGT+TGG - tig0022368:5849-5868 Msa1399470:intron 50.0%
TTGCATTTAGCTGCTCGTCC+GGG - tig0022368:5901-5920 Msa1399470:intron 50.0%
TTGAGGGTCTTTGCTGAGCA+AGG + tig0022368:5947-5966 None:intergenic 50.0%
GCTCAGCAAAGACCCTCAAT+TGG - tig0022368:5948-5967 Msa1399470:intron 50.0%
GCAAAGACCCTCAATTGGCT+CGG - tig0022368:5953-5972 Msa1399470:intron 50.0%
CTCGGAGGACTGACAAGAAA+GGG - tig0022368:5971-5990 Msa1399470:intron 50.0%
TGGCAACACAGCATTGCATG+TGG - tig0022368:6092-6111 Msa1399470:intron 50.0%
GGACATCCTCTCTCGATATG+GGG - tig0022368:6921-6940 Msa1399470:intron 50.0%
TAAGTGCCCCATATCGAGAG+AGG + tig0022368:6930-6949 None:intergenic 50.0%
CTAACGAGCTCAACCAACCA+AGG - tig0022368:6953-6972 Msa1399470:intron 50.0%
! GTTTTCCTCAGTTCGTCCCT+TGG + tig0022368:6973-6992 None:intergenic 50.0%
GTGGAGTTTCCTCAGCTCTT+TGG + tig0022368:7073-7092 None:intergenic 50.0%
AAGAGCTGAGGAAACTCCAC+AGG - tig0022368:7073-7092 Msa1399470:intron 50.0%
AGAGCTGAGGAAACTCCACA+GGG - tig0022368:7074-7093 Msa1399470:intron 50.0%
GCTGCTATATTCACCGTACC+TGG - tig0022368:7162-7181 Msa1399470:intron 50.0%
GCTATATTCACCGTACCTGG+TGG - tig0022368:7165-7184 Msa1399470:intron 50.0%
ATCGTTATCCCCACCAGGTA+CGG + tig0022368:7178-7197 None:intergenic 50.0%
!! GGGGATAACGATGATGGATC+AGG - tig0022368:7186-7205 Msa1399470:intron 50.0%
! GTTGGCTTCTGTCTGTACCT+CGG - tig0022368:7365-7384 Msa1399470:CDS 50.0%
AGAGGCAATAAATGCCACCG+AGG + tig0022368:7385-7404 None:intergenic 50.0%
GCCTCTTCGTACATAGTCGT+CGG - tig0022368:7399-7418 Msa1399470:CDS 50.0%
ACCGACGACTATGTACGAAG+AGG + tig0022368:7403-7422 None:intergenic 50.0%
CTTCGTACATAGTCGTCGGT+CGG - tig0022368:7403-7422 Msa1399470:CDS 50.0%
AGAAAGAGAAGCAGCTAGCC+AGG - tig0022368:7541-7560 Msa1399470:CDS 50.0%
! TGAGTTGGATCCACTTCTCC+TGG + tig0022368:7562-7581 None:intergenic 50.0%
GGAGAAGTGGATCCAACTCA+TGG - tig0022368:7562-7581 Msa1399470:CDS 50.0%
!! AAGACCCTCAATTGGCTCGG+AGG - tig0022368:5956-5975 Msa1399470:intron 55.0%
CAGTCCTCCGAGCCAATTGA+GGG + tig0022368:5963-5982 None:intergenic 55.0%
TCAGTCCTCCGAGCCAATTG+AGG + tig0022368:5964-5983 None:intergenic 55.0%
GCTCGGAGGACTGACAAGAA+AGG - tig0022368:5970-5989 Msa1399470:intron 55.0%
AAAAGGGCAGAGCTGTGACG+TGG - tig0022368:6017-6036 Msa1399470:intron 55.0%
CAGCATTGCATGTGGCAACC+AGG - tig0022368:6100-6119 Msa1399470:intron 55.0%
ACCAACCAAGGGACGAACTG+AGG - tig0022368:6965-6984 Msa1399470:intron 55.0%
TCCTCAGTTCGTCCCTTGGT+TGG + tig0022368:6969-6988 None:intergenic 55.0%
CTGAGGAAACTCCACAGGGA+AGG - tig0022368:7078-7097 Msa1399470:intron 55.0%
! TGCCACAAACTCGGTGACAG+TGG - tig0022368:7110-7129 Msa1399470:intron 55.0%
CACCACTGTCACCGAGTTTG+TGG + tig0022368:7115-7134 None:intergenic 55.0%
! CACAAACTCGGTGACAGTGG+TGG - tig0022368:7113-7132 Msa1399470:intron 55.0%
CCATCATCGTTATCCCCACC+AGG + tig0022368:7183-7202 None:intergenic 55.0%
CCTGGTGGGGATAACGATGA+TGG - tig0022368:7180-7199 Msa1399470:intron 55.0%
!! GAACGAGTGGGCTGCAATTC+TGG - tig0022368:7428-7447 Msa1399470:CDS 55.0%
AAGCAGCTAGCCAGGAGAAG+TGG - tig0022368:7549-7568 Msa1399470:CDS 55.0%
GGCTTCTGTCTGTACCTCGG+TGG - tig0022368:7368-7387 Msa1399470:CDS 60.0%
CGTACATAGTCGTCGGTCGG+AGG - tig0022368:7406-7425 Msa1399470:CDS 60.0%
! GACGAGAGGGCACGTCGAAG+TGG - tig0022368:5813-5832 Msa1399470:intron 65.0%
TCGTCGGTCGGAGGAACGAG+TGG - tig0022368:7415-7434 Msa1399470:CDS 65.0%
CGTCGGTCGGAGGAACGAGT+GGG - tig0022368:7416-7435 Msa1399470:CDS 65.0%
GCGGACGACGGAGATGGCGA+CGG + tig0022368:4571-4590 None:intergenic 70.0%
GCCATCTCCGTCGTCCGCGA+CGG - tig0022368:4573-4592 Msa1399470:three_prime_UTR 70.0%
GCCGTCGCGGACGACGGAGA+TGG + tig0022368:4577-4596 None:intergenic 75.0%
GACTAGAGCCGGCGCCGTCG+CGG + tig0022368:4590-4609 None:intergenic 75.0%
! TCCGTCGTCCGCGACGGCGC+CGG - tig0022368:4579-4598 Msa1399470:three_prime_UTR 80.0%
! GCCGGCGCCGTCGCGGACGA+CGG + tig0022368:4583-4602 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
tig0022368 gene 4128 8127 4128 ID=Msa1399470;Name=Msa1399470
tig0022368 mRNA 4128 8127 4128 ID=Msa1399470-mRNA-1;Parent=Msa1399470;Name=Msa1399470-mRNA-1;_AED=0.06;_eAED=0.06;_QI=234|1|1|1|0.75|0.6|5|413|593
tig0022368 exon 4128 4319 4128 ID=Msa1399470-mRNA-1:exon:17222;Parent=Msa1399470-mRNA-1
tig0022368 exon 4399 5453 4399 ID=Msa1399470-mRNA-1:exon:17221;Parent=Msa1399470-mRNA-1
tig0022368 exon 6118 6527 6118 ID=Msa1399470-mRNA-1:exon:17220;Parent=Msa1399470-mRNA-1
tig0022368 exon 7252 7764 7252 ID=Msa1399470-mRNA-1:exon:17219;Parent=Msa1399470-mRNA-1
tig0022368 exon 7869 8127 7869 ID=Msa1399470-mRNA-1:exon:17218;Parent=Msa1399470-mRNA-1
tig0022368 five_prime_UTR 7894 8127 7894 ID=Msa1399470-mRNA-1:five_prime_utr;Parent=Msa1399470-mRNA-1
tig0022368 CDS 7869 7893 7869 ID=Msa1399470-mRNA-1:cds;Parent=Msa1399470-mRNA-1
tig0022368 CDS 7252 7764 7252 ID=Msa1399470-mRNA-1:cds;Parent=Msa1399470-mRNA-1
tig0022368 CDS 6118 6527 6118 ID=Msa1399470-mRNA-1:cds;Parent=Msa1399470-mRNA-1
tig0022368 CDS 4620 5453 4620 ID=Msa1399470-mRNA-1:cds;Parent=Msa1399470-mRNA-1
tig0022368 three_prime_UTR 4399 4619 4399 ID=Msa1399470-mRNA-1:three_prime_utr;Parent=Msa1399470-mRNA-1
tig0022368 three_prime_UTR 4128 4319 4128 ID=Msa1399470-mRNA-1:three_prime_utr;Parent=Msa1399470-mRNA-1
Gene Sequence

>Msa1399470

ATGACCTCCAATTTCAACCTTCCAGGTGGAGCTCAAACTCAAAGAGATTCAGAAAAAGGAACGATGATGACACCAACACAGAACCCACTTTCCGAAATATCTCCGTCGCCATCTCCGTCGTCCGCGACGGCGCCGGCTCTAGTCCTTTCAAACTCAGGAAAACGGATCGATCAAGCAGGGAAGAAGAAGTACGTGAAGCAAGTAACGGGTCGTCACAACGACACAGAGCTTCATCTAGCAGCTCAAAGAGGTGATGTTGGTGCTGTGAGACAGATCCTTCTTGATATTGATTCTCAGATAATGGGTACTTTGAGTGGTGATGATGATGTTGATCTTAACGCTGAGATTGCTGAAGTTCGTGCTCTTGTTGTGAATGAAGAGAATGAACTTGGTGAAACTGCTTTGTTTACTGCTGCTGAAAAGGGTCATCTTGATGTTGTTAAGGAGTTGTTGAAACATTCAACTCTTCAAACTGTTTCTAAAAAGAATCGATCTGGTTTTGATCCTTTGCATATTGCTGCTAGTCAAGGACATCATGCCATTGTTCAGGTTTTACTAGATTATGACCCAAGCTTAAGCAAAACTATTGGTCCATCCAACGCCACTCCACTTATAACAGCAGCGACGAGAGGGCACGTCGAAGTGGTGAATGAACTTCTGTCAAAGGATGGCAGCTTGTTGGAAATTGCAAGATCCAATGGAAAAAATGCTTTGCATTTAGCTGCTCGTCCGGGCCATACAGAAATCGTGAAAGCCTTGCTCAGCAAAGACCCTCAATTGGCTCGGAGGACTGACAAGAAAGGGCAAACTGCATTGCATATGGCTGTAAAAGGGCAGAGCTGTGACGTGGTGAAATTGCTTCTTGATGCAGATGCTGCTATTGTTATGCTTCCTGACAAATTTGGCAACACAGCATTGCATGTGGCAACCAGGAAAAAGCGAGTAGAGATAGTGAATGAGTTGTTACTCCTGCCAGACACAAACGTAAATGCATTAACACGAGACCACAAAACAGCCCTTGACATTGCTGAAAATCTTCCTCTCTCTGAAGAGTCATCAGATATAAAGGACATCCTCTCTCGATATGGGGCACTTAGAGCTAACGAGCTCAACCAACCAAGGGACGAACTGAGGAAAACGGTTACACAAATTAAGAAAGATGTTCACACACAGCTGGAACAAACCAAGAGAACCAACAAAAATGTCCACAATATATCCAAAGAGCTGAGGAAACTCCACAGGGAAGGAATTAACAATGCCACAAACTCGGTGACAGTGGTGGCTGTGTTGTTTGCTACAGTTGCTTTTGCTGCTATATTCACCGTACCTGGTGGGGATAACGATGATGGATCAGGAGTGGTAGCGAGTTATTCTGCTTTTAAAATTTTCTTCATATTCAATGCTATTGCGCTTTTTACATCTTTGGCTGTTGTGGTTGTTCAAATTACCCTTGTTAGAGGTGAAACTAAAGCAGAGAAAAGGGTAGTTGAGGTGATTAACAAGCTCATGTGGTTGGCTTCTGTCTGTACCTCGGTGGCATTTATTGCCTCTTCGTACATAGTCGTCGGTCGGAGGAACGAGTGGGCTGCAATTCTGGTTACAGTTGTTGGAGGAGTGATAATATCCGGAGTTATTGGCACTATGACTTACTATGTAGTGAGGTCTAAGAGAACAAGATCCATGAGGAAGAAAGAGAAGCAGCTAGCCAGGAGAAGTGGATCCAACTCATGGCATCATTCAGAGTTCTCCAATTCCGAGGTTGATCGAATTTATGCGCTCTAA
Protein sequence

>Msa1399470

MTSNFNLPGGAQTQRDSEKGTMMTPTQNPLSEISPSPSPSSATAPALVLSNSGKRIDQAGKKKYVKQVTGRHNDTELHLAAQRGDVGAVRQILLDIDSQIMGTLSGDDDVDLNAEIAEVRALVVNEENELGETALFTAAEKGHLDVVKELLKHSTLQTVSKKNRSGFDPLHIAASQGHHAIVQVLLDYDPSLSKTIGPSNATPLITAATRGHVEVVNELLSKDGSLLEIARSNGKNALHLAARPGHTEIVKALLSKDPQLARRTDKKGQTALHMAVKGQSCDVVKLLLDADAAIVMLPDKFGNTALHVATRKKRVEIVNELLLLPDTNVNALTRDHKTALDIAENLPLSEESSDIKDILSRYGALRANELNQPRDELRKTVTQIKKDVHTQLEQTKRTNKNVHNISKELRKLHREGINNATNSVTVVAVLFATVAFAAIFTVPGGDNDDGSGVVASYSAFKIFFIFNAIALFTSLAVVVVQITLVRGETKAEKRVVEVINKLMWLASVCTSVAFIASSYIVVGRRNEWAAILVTVVGGVIISGVIGTMTYYVVRSKRTRSMRKKEKQLARRSGSNSWHHSEFSNSEVDRIYAL*