AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0639350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0639350 MtrunA17_Chr4g0050881 97.740 354 8 0 12 365 1 354 0.0 695
Msa0639350 MtrunA17_Chr2g0278541 58.333 348 139 2 17 358 9 356 1.01e-144 415
Msa0639350 MtrunA17_Chr2g0278551 58.859 333 135 2 17 348 9 340 4.04e-138 398
Msa0639350 MtrunA17_Chr2g0290611 54.896 337 150 2 17 352 9 344 6.45e-129 374
Msa0639350 MtrunA17_Chr4g0050891 53.727 322 146 2 17 335 10 331 6.71e-121 353
Msa0639350 MtrunA17_Chr2g0278561 52.395 334 158 1 19 351 14 347 4.77e-120 352
Msa0639350 MtrunA17_Chr4g0050941 50.541 370 169 3 1 364 1 362 1.88e-116 343
Msa0639350 MtrunA17_Chr2g0332001 50.000 346 160 4 17 353 35 376 2.22e-109 325
Msa0639350 MtrunA17_Chr4g0050931 48.204 334 129 4 19 347 11 305 1.74e-99 297
Msa0639350 MtrunA17_Chr4g0051021 42.943 333 187 3 17 348 11 341 1.76e-91 279
Msa0639350 MtrunA17_Chr4g0051001 42.904 303 172 1 17 318 28 330 6.05e-79 245
Msa0639350 MtrunA17_Chr7g0255561 37.500 344 205 4 1 341 1 337 1.56e-75 238
Msa0639350 MtrunA17_Chr7g0255581 37.500 344 205 4 1 341 1 337 1.56e-75 238
Msa0639350 MtrunA17_Chr8g0354121 38.068 352 205 9 11 354 5 351 3.37e-73 232
Msa0639350 MtrunA17_Chr3g0114691 35.854 357 216 4 10 362 10 357 2.02e-71 228
Msa0639350 MtrunA17_Chr7g0271561 37.463 339 202 5 16 346 3 339 1.22e-69 223
Msa0639350 MtrunA17_Chr3g0114731 35.756 344 215 2 20 362 18 356 2.92e-68 220
Msa0639350 MtrunA17_Chr1g0160171 35.465 344 214 3 11 351 7 345 6.39e-67 216
Msa0639350 MtrunA17_Chr2g0278021 50.224 223 107 2 133 351 1 223 8.16e-67 212
Msa0639350 MtrunA17_Chr3g0081421 36.257 342 211 6 15 352 13 351 5.43e-66 214
Msa0639350 MtrunA17_Chr1g0164831 35.503 338 215 3 20 357 3 337 3.27e-65 210
Msa0639350 MtrunA17_Chr2g0284261 34.104 346 210 5 20 357 8 343 3.35e-65 211
Msa0639350 MtrunA17_Chr3g0081511 35.756 344 214 5 20 360 17 356 7.23e-65 211
Msa0639350 MtrunA17_Chr1g0160111 36.890 328 196 4 29 348 16 340 1.02e-64 210
Msa0639350 MtrunA17_Chr3g0129571 32.687 361 224 5 17 361 4 361 8.97e-64 207
Msa0639350 MtrunA17_Chr1g0186601 36.176 340 202 8 17 351 13 342 2.90e-61 200
Msa0639350 MtrunA17_Chr1g0186571 35.043 351 215 6 17 360 10 354 3.31e-61 200
Msa0639350 MtrunA17_Chr7g0263101 34.513 339 212 5 20 356 13 343 2.61e-60 199
Msa0639350 MtrunA17_Chr3g0114711 33.333 390 212 8 17 364 11 394 1.67e-59 197
Msa0639350 MtrunA17_Chr5g0441761 35.207 338 205 4 25 356 19 348 4.96e-59 194
Msa0639350 MtrunA17_Chr3g0114701 36.467 351 214 5 12 356 1 348 1.80e-58 194
Msa0639350 MtrunA17_Chr1g0186611 35.277 343 201 9 17 351 13 342 5.95e-58 192
Msa0639350 MtrunA17_Chr3g0129591 35.952 331 200 5 18 340 3 329 9.65e-58 191
Msa0639350 MtrunA17_Chr1g0182291 32.267 344 213 6 20 357 15 344 1.30e-57 191
Msa0639350 MtrunA17_Chr3g0129601 33.739 329 217 1 17 344 26 354 6.87e-56 187
Msa0639350 MtrunA17_Chr8g0334761 31.700 347 231 3 21 364 11 354 4.75e-55 184
Msa0639350 MtrunA17_Chr1g0186641 33.623 345 218 5 11 352 4 340 1.18e-54 183
Msa0639350 MtrunA17_Chr1g0184221 31.534 352 218 4 17 352 13 357 1.26e-53 181
Msa0639350 MtrunA17_Chr1g0186561 35.588 340 199 8 17 348 13 340 6.51e-53 179
Msa0639350 MtrunA17_Chr5g0428881 32.440 336 226 1 9 343 7 342 8.00e-53 179
Msa0639350 MtrunA17_Chr5g0413961 33.333 324 200 4 33 351 27 339 1.26e-52 178
Msa0639350 MtrunA17_Chr1g0160181 35.127 353 217 6 20 365 18 365 2.17e-52 178
Msa0639350 MtrunA17_Chr4g0073241 31.844 358 220 8 21 364 9 356 6.58e-51 174
Msa0639350 MtrunA17_Chr2g0310441 31.692 325 211 3 21 340 11 329 7.11e-51 174
Msa0639350 MtrunA17_Chr6g0463971 34.049 326 205 4 20 340 16 336 8.57e-51 173
Msa0639350 MtrunA17_Chr1g0171301 31.143 350 223 6 4 348 5 341 2.23e-50 172
Msa0639350 MtrunA17_Chr8g0383731 32.951 349 218 6 20 357 5 348 3.81e-50 171
Msa0639350 MtrunA17_Chr4g0059311 31.366 322 210 5 45 363 37 350 4.16e-50 171
Msa0639350 MtrunA17_Chr3g0129581 33.333 330 210 3 18 340 3 329 8.27e-50 171
Msa0639350 MtrunA17_Chr6g0483401 32.337 368 212 8 12 364 1 346 1.27e-48 167
Msa0639350 MtrunA17_Chr3g0126101 32.424 330 206 5 21 344 7 325 2.60e-47 164
Msa0639350 MtrunA17_Chr8g0334771 31.805 349 221 5 21 360 11 351 1.99e-46 162
Msa0639350 MtrunA17_Chr3g0142131 32.486 354 212 7 25 360 25 369 4.51e-46 162
Msa0639350 MtrunA17_Chr5g0413941 31.515 330 208 4 25 346 12 331 2.27e-45 159
Msa0639350 MtrunA17_Chr5g0413951 32.219 329 199 4 25 342 12 327 3.77e-45 158
Msa0639350 MtrunA17_Chr6g0451211 31.143 350 222 5 21 363 13 350 5.39e-44 155
Msa0639350 MtrunA17_Chr3g0084091 35.918 245 147 2 100 340 11 249 5.41e-44 154
Msa0639350 MtrunA17_Chr5g0413921 32.317 328 208 4 25 346 12 331 4.80e-43 152
Msa0639350 MtrunA17_Chr8g0334751 29.972 357 222 5 21 363 12 354 5.69e-43 152
Msa0639350 MtrunA17_Chr7g0227921 32.722 327 211 4 17 340 8 328 5.84e-43 152
Msa0639350 MtrunA17_Chr1g0150151 29.223 373 235 8 1 363 1 354 1.10e-42 152
Msa0639350 MtrunA17_Chr1g0155761 30.623 369 230 8 14 361 15 378 9.13e-42 150
Msa0639350 MtrunA17_Chr5g0429001 31.970 269 181 1 77 343 9 277 5.67e-41 146
Msa0639350 MtrunA17_Chr6g0451171 32.733 333 204 8 37 365 29 345 1.96e-39 143
Msa0639350 MtrunA17_Chr5g0413931 31.412 347 219 7 24 364 10 343 1.98e-39 143
Msa0639350 MtrunA17_Chr6g0451201 32.092 349 215 7 21 363 13 345 1.21e-38 141
Msa0639350 MtrunA17_Chr5g0407971 31.025 361 209 8 16 350 7 353 2.91e-38 140
Msa0639350 MtrunA17_Chr6g0451251 32.581 310 191 5 38 340 30 328 7.08e-38 139
Msa0639350 MtrunA17_Chr1g0211801 29.297 256 164 3 20 268 11 256 3.99e-36 132
Msa0639350 MtrunA17_Chr4g0050911 47.328 131 63 2 94 218 6 136 9.36e-34 122
Msa0639350 MtrunA17_Chr6g0451261 28.758 306 212 3 18 321 9 310 1.12e-33 132
Msa0639350 MtrunA17_Chr4g0050921 54.455 101 46 0 218 318 1 101 4.82e-33 120
Msa0639350 MtrunA17_Chr8g0369841 29.446 343 222 6 25 351 14 352 4.83e-33 126
Msa0639350 MtrunA17_Chr2g0278031 50.435 115 56 1 19 132 13 127 6.37e-32 117
Msa0639350 MtrunA17_Chr5g0408031 28.804 368 230 6 17 363 7 363 5.41e-31 121
Msa0639350 MtrunA17_Chr5g0408041 28.338 367 231 6 19 364 10 365 5.91e-31 121
Msa0639350 MtrunA17_Chr3g0126091 27.928 333 223 6 21 343 12 337 1.24e-30 119
Msa0639350 MtrunA17_Chr1g0177221 32.806 253 160 4 20 268 18 264 2.26e-30 117
Msa0639350 MtrunA17_Chr5g0407991 28.610 367 224 9 20 363 9 360 3.88e-30 119
Msa0639350 MtrunA17_Chr5g0408001 28.493 365 235 9 12 357 1 358 1.49e-29 117
Msa0639350 MtrunA17_Chr5g0404811 30.992 242 152 5 133 363 7 244 2.96e-28 110
Msa0639350 MtrunA17_Chr6g0451191 29.114 237 147 5 133 365 1 220 2.98e-28 110
Msa0639350 MtrunA17_Chr4g0056081 27.684 354 213 11 25 353 19 354 1.12e-27 111
Msa0639350 MtrunA17_Chr5g0399281 26.074 349 235 7 25 363 13 348 2.77e-27 110
Msa0639350 MtrunA17_Chr8g0334711 33.990 203 116 4 21 215 11 203 9.82e-27 105
Msa0639350 MtrunA17_Chr8g0334721 31.655 139 89 2 232 364 1 139 4.09e-22 91.7
Msa0639350 MtrunA17_Chr1g0182261 36.301 146 86 5 209 351 76 217 1.07e-18 84.0
Msa0639350 MtrunA17_Chr1g0177231 42.667 75 43 0 268 342 28 102 1.94e-15 72.4
Msa0639350 MtrunA17_Chr1g0172891 46.970 66 35 0 275 340 13 78 6.37e-15 70.5
Msa0639350 MtrunA17_Chr4g0050901 60.000 65 23 1 12 73 1 65 1.86e-14 67.8
Msa0639350 MtrunA17_Chr8g0334731 29.717 212 121 6 21 230 12 197 3.38e-14 70.9
Msa0639350 MtrunA17_Chr1g0211811 37.838 74 46 0 272 345 3 76 6.55e-14 67.0
Msa0639350 MtrunA17_Chr6g0451241 39.362 94 54 1 270 363 9 99 1.33e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0639350 AT1G25270.1 40.462 346 200 4 19 362 1 342 6.61e-81 251
Msa0639350 AT1G68170.2 41.390 331 193 1 11 340 3 333 2.62e-75 237
Msa0639350 AT1G68170.1 42.105 323 186 1 19 340 1 323 4.54e-75 236
Msa0639350 AT4G08290.1 36.517 356 217 3 17 364 9 363 2.08e-72 230
Msa0639350 AT1G44800.1 37.322 351 208 5 10 357 1 342 1.27e-68 220
Msa0639350 AT5G07050.1 36.827 353 212 6 17 358 14 366 3.33e-67 218
Msa0639350 AT1G09380.1 34.615 364 210 5 21 364 8 363 4.33e-67 216
Msa0639350 AT2G37460.1 33.908 348 216 5 20 364 14 350 8.62e-66 213
Msa0639350 AT4G08300.1 35.244 349 220 3 10 357 1 344 9.02e-65 210
Msa0639350 AT1G21890.1 34.916 358 215 4 17 365 7 355 2.17e-64 210
Msa0639350 AT5G13670.1 35.692 325 202 6 20 340 7 328 1.30e-63 207
Msa0639350 AT2G39510.1 34.571 350 213 6 20 365 7 344 3.32e-62 204
Msa0639350 AT3G30340.1 34.421 337 207 4 20 349 10 339 3.84e-61 201
Msa0639350 AT4G30420.2 32.880 368 217 5 20 364 8 368 8.59e-59 195
Msa0639350 AT4G30420.1 32.782 363 214 5 25 364 3 358 4.96e-57 190
Msa0639350 AT1G01070.1 32.845 341 222 4 20 358 15 350 8.33e-57 189
Msa0639350 AT2G40900.1 33.828 337 207 6 15 344 5 332 9.42e-56 187
Msa0639350 AT4G01440.1 32.440 336 223 2 12 346 1 333 1.13e-55 186
Msa0639350 AT5G64700.1 34.593 344 216 5 17 351 4 347 1.16e-55 186
Msa0639350 AT1G21890.2 35.256 312 195 2 17 321 7 318 1.08e-53 181
Msa0639350 AT3G56620.1 32.110 327 210 3 14 337 4 321 5.92e-53 180
Msa0639350 AT1G11450.3 31.471 340 225 4 21 357 16 350 3.18e-52 177
Msa0639350 AT1G43650.1 34.146 328 201 4 20 342 6 323 2.07e-51 175
Msa0639350 AT4G01430.1 32.132 333 214 7 21 346 9 336 9.98e-51 174
Msa0639350 AT3G18200.2 31.818 352 214 11 11 351 23 359 1.81e-50 173
Msa0639350 AT4G01440.3 32.143 308 205 2 12 318 1 305 8.68e-50 170
Msa0639350 AT3G53210.1 31.728 353 232 3 12 357 1 351 8.90e-50 171
Msa0639350 AT1G11460.1 32.609 322 212 4 21 340 16 334 5.44e-49 168
Msa0639350 AT2G37450.2 32.036 334 192 5 12 340 1 304 5.65e-49 168
Msa0639350 AT1G11450.5 31.833 311 204 4 50 357 1 306 5.80e-48 165
Msa0639350 AT4G01450.2 33.939 330 210 3 12 340 1 323 4.12e-47 164
Msa0639350 AT5G40240.1 31.420 331 224 2 16 344 15 344 2.33e-46 162
Msa0639350 AT5G40240.3 31.420 331 224 2 16 344 15 344 2.33e-46 162
Msa0639350 AT4G01450.1 33.442 308 197 3 12 318 1 301 3.31e-46 161
Msa0639350 AT5G40240.2 31.902 326 219 2 21 344 34 358 3.73e-46 162
Msa0639350 AT1G01070.2 31.803 305 202 3 55 358 4 303 4.00e-46 160
Msa0639350 AT4G08290.2 35.662 272 169 3 17 287 9 275 6.40e-46 159
Msa0639350 AT4G28040.1 31.063 367 215 7 20 363 7 358 6.81e-45 158
Msa0639350 AT4G28040.5 31.063 367 215 7 20 363 7 358 6.81e-45 158
Msa0639350 AT4G28040.3 31.063 367 215 7 20 363 7 358 6.81e-45 158
Msa0639350 AT4G28040.4 31.063 367 215 7 20 363 7 358 6.81e-45 158
Msa0639350 AT4G28040.2 31.063 367 215 7 20 363 7 358 6.81e-45 158
Msa0639350 AT1G11450.2 31.250 304 201 4 57 357 1 299 2.90e-44 155
Msa0639350 AT3G53210.2 33.209 268 171 2 96 357 29 294 1.30e-42 150
Msa0639350 AT1G75500.1 31.250 352 224 9 17 357 16 360 1.48e-42 152
Msa0639350 AT1G75500.2 31.250 352 224 9 17 357 16 360 1.48e-42 152
Msa0639350 AT5G40230.1 31.611 329 216 5 21 344 21 345 1.07e-41 150
Msa0639350 AT1G11450.4 30.897 301 200 4 21 318 16 311 1.13e-41 148
Msa0639350 AT3G30340.2 35.294 255 152 3 20 268 10 257 7.46e-41 145
Msa0639350 AT4G01450.4 34.109 258 162 3 12 268 1 251 2.08e-40 144
Msa0639350 AT4G01430.2 30.820 305 199 7 21 318 9 308 4.81e-40 144
Msa0639350 AT1G70260.1 28.736 348 214 8 17 344 8 341 5.99e-40 145
Msa0639350 AT3G18200.1 33.193 238 144 6 118 351 3 229 2.24e-39 140
Msa0639350 AT4G01440.5 32.456 228 151 1 119 346 1 225 3.48e-39 140
Msa0639350 AT4G01440.2 32.456 228 151 1 119 346 1 225 3.48e-39 140
Msa0639350 AT2G37450.3 33.613 238 144 3 108 340 4 232 3.60e-39 140
Msa0639350 AT4G15540.1 31.805 349 199 7 21 359 17 336 6.27e-39 142
Msa0639350 AT4G15540.2 31.805 349 199 7 21 359 17 336 6.27e-39 142
Msa0639350 AT5G40210.1 33.437 323 187 9 36 351 28 329 6.70e-39 142
Msa0639350 AT3G28050.1 30.294 340 217 7 36 363 27 358 1.26e-38 142
Msa0639350 AT4G01440.4 32.558 258 170 2 12 268 1 255 1.90e-38 139
Msa0639350 AT5G45370.1 30.435 345 202 6 25 357 24 342 7.58e-38 139
Msa0639350 AT5G45370.2 29.830 352 219 5 25 357 24 366 3.67e-37 138
Msa0639350 AT5G45370.4 29.830 352 219 5 25 357 24 366 3.67e-37 138
Msa0639350 AT1G11450.1 31.250 256 169 3 104 357 8 258 1.46e-36 133
Msa0639350 AT3G28070.2 33.333 255 159 6 102 351 7 255 5.99e-36 132
Msa0639350 AT3G45870.1 31.098 328 210 5 20 335 11 334 8.21e-36 134
Msa0639350 AT3G28070.1 32.830 265 167 6 92 351 89 347 3.05e-35 132
Msa0639350 AT3G28100.1 30.599 317 210 6 36 347 29 340 1.52e-33 127
Msa0639350 AT4G01450.3 34.361 227 141 3 12 237 1 220 2.86e-33 124
Msa0639350 AT5G64700.2 32.422 256 168 4 17 267 4 259 2.69e-32 122
Msa0639350 AT1G60050.1 26.273 373 243 8 12 363 4 365 8.43e-32 123
Msa0639350 AT3G28080.5 31.950 241 157 4 108 345 13 249 1.78e-31 120
Msa0639350 AT3G28080.7 31.950 241 157 4 108 345 13 249 1.78e-31 120
Msa0639350 AT3G28080.8 31.950 241 157 4 108 345 13 249 1.78e-31 120
Msa0639350 AT3G28080.6 31.950 241 157 4 108 345 13 249 1.78e-31 120
Msa0639350 AT3G28130.5 30.252 238 163 3 108 344 15 250 4.55e-31 119
Msa0639350 AT3G28130.1 30.252 238 163 3 108 344 15 250 4.55e-31 119
Msa0639350 AT3G53210.3 31.707 246 165 2 12 256 1 244 4.98e-31 119
Msa0639350 AT3G28080.1 31.950 241 157 4 108 345 102 338 7.67e-31 120
Msa0639350 AT3G28080.2 31.950 241 157 4 108 345 94 330 1.01e-30 120
Msa0639350 AT3G28130.2 30.769 234 159 3 108 340 101 332 2.23e-30 119
Msa0639350 AT4G19185.1 30.678 339 213 8 25 346 25 358 1.75e-29 117
Msa0639350 AT2G37450.1 31.455 213 132 3 133 340 62 265 1.86e-29 115
Msa0639350 AT5G45370.6 30.739 257 153 3 104 342 2 251 2.60e-29 114
Msa0639350 AT5G45370.5 30.739 257 153 3 104 342 2 251 2.60e-29 114
Msa0639350 AT5G47470.1 28.242 347 226 8 25 364 34 364 3.02e-29 116
Msa0639350 AT5G47470.2 28.242 347 226 8 25 364 62 392 4.74e-29 116
Msa0639350 AT3G45870.8 29.260 311 204 5 20 318 11 317 5.50e-28 112
Msa0639350 AT1G43650.2 32.411 253 156 4 20 267 6 248 8.32e-28 110
Msa0639350 AT4G01430.3 31.863 204 129 5 148 346 1 199 1.06e-27 108
Msa0639350 AT4G16620.1 30.097 309 196 8 59 359 49 345 2.45e-27 111
Msa0639350 AT5G47470.3 27.964 329 218 7 25 346 62 378 2.73e-27 111
Msa0639350 AT3G28070.5 31.839 223 141 6 134 351 2 218 3.76e-27 107
Msa0639350 AT4G24980.1 27.974 311 147 9 36 341 2 240 3.86e-27 108
Msa0639350 AT4G19185.2 31.298 262 165 5 98 346 21 280 1.47e-26 108
Msa0639350 AT3G28070.3 31.897 232 147 6 92 318 89 314 3.39e-24 101
Msa0639350 AT3G45870.5 31.429 210 135 2 134 335 51 259 3.80e-23 98.6
Msa0639350 AT4G16620.4 29.183 257 165 5 108 359 4 248 5.42e-23 97.1
Msa0639350 AT4G16620.2 29.183 257 165 5 108 359 4 248 5.42e-23 97.1
Msa0639350 AT3G28080.9 29.767 215 144 4 134 345 2 212 6.45e-23 96.3
Msa0639350 AT5G45370.3 29.075 227 136 3 134 342 74 293 7.45e-23 97.8
Msa0639350 AT3G28130.3 28.846 208 145 3 134 340 2 207 8.20e-23 95.9
Msa0639350 AT3G45870.9 29.719 249 160 4 20 256 11 256 1.41e-20 90.9
Msa0639350 AT3G28080.3 27.917 240 114 4 108 345 102 284 4.42e-18 84.3
Msa0639350 AT3G45870.2 34.286 140 91 1 196 335 45 183 5.14e-18 82.8
Msa0639350 AT3G45870.6 28.497 193 129 2 134 318 51 242 9.97e-16 76.6
Msa0639350 AT3G45870.7 28.497 193 129 2 134 318 51 242 9.97e-16 76.6
Msa0639350 AT3G28100.3 28.390 236 160 5 36 266 29 260 4.00e-13 69.3
Msa0639350 AT4G16620.3 28.934 197 132 5 59 252 49 240 6.37e-13 68.9
Msa0639350 AT3G28080.4 33.735 83 54 1 263 345 114 195 1.05e-11 64.3

Find 106 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCATACTTCCCTTCCTTAA+TGG 0.146793 4_3:-35027915 Msa0639350:exon
TTGTCTTGGCCGTGTCTTTC+GGG 0.189585 4_3:-35027176 Msa0639350:intron
AAGGAGTAGAATTTGATATT+TGG 0.219912 4_3:-35026953 Msa0639350:CDS
CGTTCCGTGTGGTCGTTCTT+TGG 0.231574 4_3:+35026906 None:intergenic
CTCAATCTGGCTCATCATTC+AGG 0.236067 4_3:-35026820 Msa0639350:intron
GTTGCTTCTGTTTCTCAATC+TGG 0.266172 4_3:-35026833 Msa0639350:CDS
GGAAGCTTGGTTGGGATATC+AGG 0.284815 4_3:-35026593 Msa0639350:CDS
GCAATAGACCCCAGCCATTA+AGG 0.287649 4_3:+35027901 None:intergenic
TTTGTCTTGGCCGTGTCTTT+CGG 0.313966 4_3:-35027177 Msa0639350:CDS
TACTTCCCTTCCTTAATGGC+TGG 0.316974 4_3:-35027911 Msa0639350:exon
CAAAAGTGACGGAGGGAATA+AGG 0.317897 4_3:+35027196 None:intergenic
GGAACTATACTTGGATTTGC+TGG 0.338162 4_3:-35026999 Msa0639350:CDS
GATACTCATTCCATCATATA+TGG 0.347161 4_3:+35027772 None:intergenic
CAACAATGGCCTTGCAGCTT+CGG 0.348823 4_3:+35025951 None:intergenic
GCTACAATCAAAAGCATTAG+AGG 0.354140 4_3:+35026349 None:intergenic
ATAGCTTTGATGTCTGTAAT+AGG 0.358630 4_3:-35026669 Msa0639350:CDS
CTTGAAACCAGCCTTGCTAA+TGG 0.382121 4_3:-35027843 Msa0639350:CDS
GCCATTAAGGAAGGGAAGTA+TGG 0.382507 4_3:+35027914 None:intergenic
ACTTCCCTTCCTTAATGGCT+GGG 0.392567 4_3:-35027910 Msa0639350:exon
CATTAGCAAGGCTGGTTTCA+AGG 0.401211 4_3:+35027844 None:intergenic
ACTTGGATTTGCTGGGGCAT+TGG 0.417474 4_3:-35026991 Msa0639350:CDS
GCCGGACCCAATCCACTTAC+CGG 0.420185 4_3:-35029778 Msa0639350:intron
GGAGGAGTGTTGATTGTTTG+TGG 0.420620 4_3:-35026054 Msa0639350:CDS
TTGAAAGCAGACGCATATAA+AGG 0.425809 4_3:+35026373 None:intergenic
AACCAGCCTTGCTAATGGTT+GGG 0.425888 4_3:-35027838 Msa0639350:CDS
TGGCTTATATGCGGTGCTAT+GGG 0.429544 4_3:-35026034 Msa0639350:CDS
GTGGCTTATATGCGGTGCTA+TGG 0.439586 4_3:-35026035 Msa0639350:CDS
TTTCGTGTGTATAGTATACT+AGG 0.440858 4_3:-35026075 Msa0639350:intron
GATGCTACCACTGAATTGTT+AGG 0.445239 4_3:-35026876 Msa0639350:CDS
ACCCCAACCATTAGCAAGGC+TGG 0.447222 4_3:+35027836 None:intergenic
ATTAAATTGGCGAGCAGTAA+CGG 0.448622 4_3:-35027039 Msa0639350:CDS
AAACAAGAGCTCCGTAGAAT+AGG 0.449858 4_3:+35027253 None:intergenic
TGAACCAGTGGAAGCTTGGT+TGG 0.454016 4_3:-35026602 Msa0639350:CDS
TCCAAAAGATTGATTGCAAA+AGG 0.456878 4_3:+35026930 None:intergenic
AACACTCTCCAAGTCATCTT+CGG 0.457047 4_3:+35027439 None:intergenic
TTAAATTGGCGAGCAGTAAC+GGG 0.459220 4_3:-35027038 Msa0639350:CDS
GAACCAGTGGAAGCTTGGTT+GGG 0.459485 4_3:-35026601 Msa0639350:CDS
TACGTACCGGTAAGTGGATT+GGG 0.474662 4_3:+35029772 None:intergenic
TTGCAGTCTGATCTTGAAGA+TGG 0.475617 4_3:-35027882 Msa0639350:CDS
TACAAGCTTGCCATATATGA+TGG 0.476535 4_3:-35027782 Msa0639350:CDS
GATTTGAACCAGTGGAAGCT+TGG 0.483175 4_3:-35026606 Msa0639350:CDS
AAACCAGCCTTGCTAATGGT+TGG 0.486693 4_3:-35027839 Msa0639350:CDS
AAGGTTGTAGGAACTATACT+TGG 0.488678 4_3:-35027008 Msa0639350:CDS
GAACTATACTTGGATTTGCT+GGG 0.489267 4_3:-35026998 Msa0639350:CDS
CAATGTTACGTACCGGTAAG+TGG 0.492691 4_3:+35029766 None:intergenic
ACCGGTAAGTGGATTGGGTC+CGG 0.494650 4_3:+35029777 None:intergenic
TGGAATAATGATTCTTGTCA+TGG 0.496034 4_3:-35026419 Msa0639350:CDS
TTACGTACCGGTAAGTGGAT+TGG 0.496608 4_3:+35029771 None:intergenic
GTGTTTGTCCTCAGTACCTC+TGG 0.499066 4_3:+35029918 None:intergenic
TTATTTCAAAACCTATTCTA+CGG 0.499151 4_3:-35027264 Msa0639350:CDS
CTTCCCTTCCTTAATGGCTG+GGG 0.501081 4_3:-35027909 Msa0639350:exon
ACGGGAAAGGCAAAGGTTGT+AGG 0.503442 4_3:-35027020 Msa0639350:CDS
GGAATCACCAATGATTTCTG+AGG 0.504240 4_3:+35025972 None:intergenic
ATTCGTCGCATCCATGCTCT+TGG 0.505539 4_3:-35026326 Msa0639350:CDS
GGAAGAGACCGAAGATGACT+TGG 0.506957 4_3:-35027447 Msa0639350:CDS
GGATTGGGTCCGGCTCTTCG+AGG 0.509683 4_3:+35029787 None:intergenic
TAATGATTCTTGTCATGGCA+TGG 0.509769 4_3:-35026414 Msa0639350:CDS
AATAAGAGCAAGAGGAATAG+TGG 0.511175 4_3:+35027709 None:intergenic
ATCAAAGCTATGCTTGATTG+AGG 0.515025 4_3:+35026681 None:intergenic
TTAGAGCCCTCAGAAATCAT+TGG 0.522947 4_3:-35025979 Msa0639350:CDS
TATATAATATATACCTTCCG+AGG 0.524794 4_3:+35026290 None:intergenic
TATGCGGTGCTATGGGGTAA+AGG 0.526858 4_3:-35026027 Msa0639350:CDS
ATAGCAACTCCTCGAAGAGC+CGG 0.527327 4_3:-35029796 Msa0639350:five_prime_UTR
GCTATGCTTGATTGAGGGCA+TGG 0.539116 4_3:+35026687 None:intergenic
ACTGAGGACAAACACGCGCC+AGG 0.545882 4_3:-35029910 Msa0639350:five_prime_UTR
ACCAGCCTTGCTAATGGTTG+GGG 0.550467 4_3:-35027837 Msa0639350:CDS
CTTTCGAAAATAAGAGCAAG+AGG 0.553483 4_3:+35027701 None:intergenic
GCATGGGTCGTACAGAAGAA+AGG 0.553756 4_3:-35026397 Msa0639350:CDS
TCAAAGCTATGCTTGATTGA+GGG 0.556100 4_3:+35026682 None:intergenic
TACCTCTGGTACACTAAGCC+TGG 0.559669 4_3:+35029892 None:intergenic
TGCAGTCTGATCTTGAAGAT+GGG 0.567743 4_3:-35027881 Msa0639350:CDS
TATCCCAACCAAGCTTCCAC+TGG 0.569343 4_3:+35026598 None:intergenic
AATGATTCTTGTCATGGCAT+GGG 0.571068 4_3:-35026413 Msa0639350:CDS
TAACTTCTCATCCAAGAGCA+TGG 0.575858 4_3:+35026315 None:intergenic
TAGACCCCAGCCATTAAGGA+AGG 0.576214 4_3:+35027905 None:intergenic
ATAATCCACTTAGAAGTGAA+AGG 0.580234 4_3:+35027413 None:intergenic
CGCTGCAAAGGCGAGACGAT+AGG 0.583202 4_3:+35027736 None:intergenic
AATAGTGGTAACCGCTGCAA+AGG 0.587272 4_3:+35027724 None:intergenic
GAATCACCAATGATTTCTGA+GGG 0.590585 4_3:+35025973 None:intergenic
TTGGCGAGCAGTAACGGGAA+AGG 0.593835 4_3:-35027033 Msa0639350:CDS
AGCAGTAACGGGAAAGGCAA+AGG 0.594780 4_3:-35027027 Msa0639350:CDS
AACTATACTTGGATTTGCTG+GGG 0.597676 4_3:-35026997 Msa0639350:CDS
ATGAGAAGTTAAACCTCGGA+AGG 0.598102 4_3:-35026303 Msa0639350:intron
CTATCGTCTCGCCTTTGCAG+CGG 0.608374 4_3:-35027735 Msa0639350:CDS
CACGGCCAAGACAAAAGTGA+CGG 0.610470 4_3:+35027185 None:intergenic
TTGCACCCCAACCATTAGCA+AGG 0.612822 4_3:+35027832 None:intergenic
TAAATGAGCATCGATGCTCA+TGG 0.613254 4_3:+35025925 None:intergenic
GGCTTATATGCGGTGCTATG+GGG 0.613745 4_3:-35026033 Msa0639350:CDS
CATCGATGCTCATTTAACAC+AGG 0.615170 4_3:-35025918 None:intergenic
TTGGTGATTCCGAAGCTGCA+AGG 0.619485 4_3:-35025960 Msa0639350:CDS
GATGCTCATGGACTCAACAA+TGG 0.622052 4_3:+35025937 None:intergenic
GCCAAGACAAAAGTGACGGA+GGG 0.626370 4_3:+35027189 None:intergenic
GGCCAAGACAAAAGTGACGG+AGG 0.627973 4_3:+35027188 None:intergenic
TTGAGAGGGATTTGAACCAG+TGG 0.633369 4_3:-35026614 Msa0639350:CDS
CAGTAAACACAAAGCACCAG+AGG 0.643966 4_3:-35029934 Msa0639350:five_prime_UTR
AGACCCCAGCCATTAAGGAA+GGG 0.648693 4_3:+35027906 None:intergenic
TAGAACACCTAACAATTCAG+TGG 0.656150 4_3:+35026869 None:intergenic
GATTGTTTGTGGCTTATATG+CGG 0.657640 4_3:-35026043 Msa0639350:CDS
ACAAAGCACCAGAGGTACTG+AGG 0.658480 4_3:-35029926 Msa0639350:five_prime_UTR
CGCCAGGCTTAGTGTACCAG+AGG 0.659080 4_3:-35029894 Msa0639350:five_prime_UTR
CGTGTGTATAGTATACTAGG+AGG 0.659289 4_3:-35026072 Msa0639350:intron
ATCGATGCTCATTTAACACA+GGG 0.659875 4_3:-35025917 None:intergenic
GGATCCAAAGAACGACCACA+CGG 0.660238 4_3:-35026910 Msa0639350:CDS
TTGGATGAGAAGTTAAACCT+CGG 0.681264 4_3:-35026307 Msa0639350:CDS
TTAAACATACCCGAAAGACA+CGG 0.692447 4_3:+35027167 None:intergenic
CATCAGTGACACGTTCCGTG+TGG 0.704527 4_3:+35026895 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTATTTTTAAAATAAATTG+TGG + chr4_3:35027009-35027028 None:intergenic 10.0%
!!! AAAATTTTGTCAAAAAAAAA+TGG + chr4_3:35027138-35027157 None:intergenic 10.0%
!! CATAATAATTCAAAAAAATT+AGG + chr4_3:35027635-35027654 None:intergenic 10.0%
!! CAATAATAAATAAAACTAAA+AGG + chr4_3:35027843-35027862 None:intergenic 10.0%
!!! TTTAGTTTTATTTATTATTG+TGG - chr4_3:35027843-35027862 Msa0639350:CDS 10.0%
!!! TTAGTTTTATTTATTATTGT+GGG - chr4_3:35027844-35027863 Msa0639350:CDS 10.0%
!!! TTAGAGGTTATAATTTTTTT+AGG - chr4_3:35026153-35026172 Msa0639350:intron 15.0%
!! AAATAATTGAAATACTATGT+TGG + chr4_3:35026311-35026330 None:intergenic 15.0%
!! AACATTGCATAATATATTTA+GGG + chr4_3:35026452-35026471 None:intergenic 15.0%
!!! TTTTAAGAAACTAGTTTTTT+GGG - chr4_3:35027064-35027083 Msa0639350:intron 15.0%
!! TTAATGCAAGTATATTAATA+TGG + chr4_3:35027167-35027186 None:intergenic 15.0%
!!! AAAGGATGAAAATTTTATAA+TGG + chr4_3:35027825-35027844 None:intergenic 15.0%
!!! ATTATAATTTAATTTCCCTT+AGG - chr4_3:35027880-35027899 Msa0639350:CDS 15.0%
!! GAAATTCAATATAAAGAATA+TGG + chr4_3:35028282-35028301 None:intergenic 15.0%
!! AATGACTTATATATATATAC+AGG - chr4_3:35028406-35028425 Msa0639350:intron 15.0%
!!! GTTAATTGTTTTAATTTACA+TGG - chr4_3:35029138-35029157 Msa0639350:intron 15.0%
!! TTAATGTGTTAATTATTTGA+AGG - chr4_3:35029419-35029438 Msa0639350:intron 15.0%
!! TAATGTGTTAATTATTTGAA+GGG - chr4_3:35029420-35029439 Msa0639350:intron 15.0%
!!! TTTTGTTGTATCTATTTTCT+TGG + chr4_3:35026010-35026029 None:intergenic 20.0%
!!! GTTATAATTTTTTTAGGTCA+TGG - chr4_3:35026159-35026178 Msa0639350:intron 20.0%
!! GAACATTGCATAATATATTT+AGG + chr4_3:35026453-35026472 None:intergenic 20.0%
!! TATTAGAAAATTGTGAAAAG+TGG - chr4_3:35026656-35026675 Msa0639350:CDS 20.0%
!! TGCAAGACTATTAAAAATAT+GGG + chr4_3:35026779-35026798 None:intergenic 20.0%
!! ATAAACTTGATTTAAATTGC+TGG + chr4_3:35026819-35026838 None:intergenic 20.0%
!! AACTATATTCTTCAATGTTA+CGG + chr4_3:35026876-35026895 None:intergenic 20.0%
!! AATACGGATGATAATAAAAA+TGG + chr4_3:35026971-35026990 None:intergenic 20.0%
!!! CTTTTAAGAAACTAGTTTTT+TGG - chr4_3:35027063-35027082 Msa0639350:intron 20.0%
!! AAATGGTTCAAAATTTAATC+CGG + chr4_3:35027121-35027140 None:intergenic 20.0%
!!! AAAATATTTCAACGTGTTTT+TGG + chr4_3:35027556-35027575 None:intergenic 20.0%
!!! TTGAATTTTAGAATCCAAAT+AGG - chr4_3:35027574-35027593 Msa0639350:intron 20.0%
!! TAATGAAAATGAAAACCTAA+GGG + chr4_3:35027898-35027917 None:intergenic 20.0%
!! ATAATGAAAATGAAAACCTA+AGG + chr4_3:35027899-35027918 None:intergenic 20.0%
!!! GGTAATTAATTTGAAACAAT+AGG - chr4_3:35028195-35028214 Msa0639350:intron 20.0%
!! AAGAAAATTTATATGATGAG+AGG + chr4_3:35028304-35028323 None:intergenic 20.0%
!! GCAAAAAAATCAAACTATAT+AGG - chr4_3:35028324-35028343 Msa0639350:intron 20.0%
!! TTATTTCAAAACCTATTCTA+CGG - chr4_3:35028610-35028629 Msa0639350:intron 20.0%
!! ATTTCACAAATCATATGATA+TGG + chr4_3:35029369-35029388 None:intergenic 20.0%
!!! TTTTTTTAGGTCATGGTAAT+CGG - chr4_3:35026166-35026185 Msa0639350:intron 25.0%
! CACACTTATTCATCTTATAA+AGG + chr4_3:35026413-35026432 None:intergenic 25.0%
! CTTTATAAGATGAATAAGTG+TGG - chr4_3:35026411-35026430 Msa0639350:CDS 25.0%
! CTGCAAGACTATTAAAAATA+TGG + chr4_3:35026780-35026799 None:intergenic 25.0%
!! ATGAATTGTTGTTAGTTTAG+TGG - chr4_3:35026837-35026856 Msa0639350:CDS 25.0%
! GGAAAAATTATTATCCATAG+TGG + chr4_3:35026929-35026948 None:intergenic 25.0%
! GATAGTAAAAGAAAATGGTT+TGG + chr4_3:35027091-35027110 None:intergenic 25.0%
! AACATTCATGCATTAAGTAT+TGG - chr4_3:35027197-35027216 Msa0639350:CDS 25.0%
! ACATTCATGCATTAAGTATT+GGG - chr4_3:35027198-35027217 Msa0639350:CDS 25.0%
!! AGTATTTTACACGTGTTTTT+AGG + chr4_3:35027297-35027316 None:intergenic 25.0%
! TTAGTGATAGTTAACTATCT+GGG + chr4_3:35027328-35027347 None:intergenic 25.0%
! AACATAAAACAAATGAACGT+TGG + chr4_3:35027754-35027773 None:intergenic 25.0%
!!! GTAGTTTTGAGGAATTAAAT+TGG - chr4_3:35028822-35028841 Msa0639350:intron 25.0%
! AAGGAGTAGAATTTGATATT+TGG - chr4_3:35028921-35028940 Msa0639350:intron 25.0%
!!! TGTTTTAATTTACATGGCAT+AGG - chr4_3:35029144-35029163 Msa0639350:intron 25.0%
!!! GCAATACAATGTGTTATTTT+TGG - chr4_3:35029229-35029248 Msa0639350:intron 25.0%
! CTAATTCACAACTAGAATTA+TGG - chr4_3:35029343-35029362 Msa0639350:intron 25.0%
! TATATAATATATACCTTCCG+AGG + chr4_3:35029587-35029606 None:intergenic 25.0%
! TTCATTTAAATGAGTATGCA+TGG - chr4_3:35029618-35029637 Msa0639350:intron 25.0%
! ACCAATTTGAATGTTGTTAT+TGG - chr4_3:35029659-35029678 Msa0639350:intron 25.0%
! TCCAATAACAACATTCAAAT+TGG + chr4_3:35029663-35029682 None:intergenic 25.0%
! AAATCAAGTTATGCATACAA+AGG + chr4_3:35029758-35029777 None:intergenic 25.0%
AACAACTAAAAACACAACCA+AGG - chr4_3:35026037-35026056 Msa0639350:CDS 30.0%
AATGATTCAATGTTACGTAC+CGG + chr4_3:35026118-35026137 None:intergenic 30.0%
!!! TAGATGTCTTGTTTTGTTAG+AGG - chr4_3:35026137-35026156 Msa0639350:intron 30.0%
TTCAATATTGTGTTGGAGAA+TGG + chr4_3:35026237-35026256 None:intergenic 30.0%
TGAAATACTATGTTGGAGAA+TGG + chr4_3:35026304-35026323 None:intergenic 30.0%
! CATTGTAAGTTTAGCTTAGT+TGG + chr4_3:35026479-35026498 None:intergenic 30.0%
TAGTTCAAATCTCATTCACA+TGG - chr4_3:35026891-35026910 Msa0639350:CDS 30.0%
GGTTATAAAAGAATTGACGA+TGG + chr4_3:35026950-35026969 None:intergenic 30.0%
GGTTGGATAGTAAAAGAAAA+TGG + chr4_3:35027096-35027115 None:intergenic 30.0%
!!! ATTGTGTTTTGGTAAAGACA+TGG + chr4_3:35027245-35027264 None:intergenic 30.0%
! AAGCAACTTTCATTGTGTTT+TGG + chr4_3:35027256-35027275 None:intergenic 30.0%
TAGTGATAGTTAACTATCTG+GGG + chr4_3:35027327-35027346 None:intergenic 30.0%
GTTAGTGATAGTTAACTATC+TGG + chr4_3:35027329-35027348 None:intergenic 30.0%
!! TTAAGGGGTTTTATGCAATT+CGG + chr4_3:35027528-35027547 None:intergenic 30.0%
!!! GTGTTTTTGGAAGTTTTAAG+GGG + chr4_3:35027543-35027562 None:intergenic 30.0%
!!! CGTGTTTTTGGAAGTTTTAA+GGG + chr4_3:35027544-35027563 None:intergenic 30.0%
!!! ACGTGTTTTTGGAAGTTTTA+AGG + chr4_3:35027545-35027564 None:intergenic 30.0%
! AAAACAAATGAACGTTGGTT+CGG + chr4_3:35027749-35027768 None:intergenic 30.0%
GATACTCATTCCATCATATA+TGG + chr4_3:35028105-35028124 None:intergenic 30.0%
CATTATCAGTTGTGAATAGT+TGG + chr4_3:35028390-35028409 None:intergenic 30.0%
! TTTTACCTTTCACTTCTAAG+TGG - chr4_3:35028456-35028475 Msa0639350:intron 30.0%
ATAATCCACTTAGAAGTGAA+AGG + chr4_3:35028464-35028483 None:intergenic 30.0%
TTGCATTGCATCACATATAT+AGG + chr4_3:35028570-35028589 None:intergenic 30.0%
!!! TTGGTGTTGACATTTTACAA+AGG - chr4_3:35028902-35028921 Msa0639350:intron 30.0%
TCCAAAAGATTGATTGCAAA+AGG + chr4_3:35028947-35028966 None:intergenic 30.0%
!! ATAGCTTTGATGTCTGTAAT+AGG - chr4_3:35029205-35029224 Msa0639350:intron 30.0%
!!! TTTTTGGTTTCATTGTTGAG+AGG - chr4_3:35029245-35029264 Msa0639350:intron 30.0%
!!! TTTTGGTTTCATTGTTGAGA+GGG - chr4_3:35029246-35029265 Msa0639350:intron 30.0%
!! GTGAAATTGTTGACTCATAA+AGG - chr4_3:35029382-35029401 Msa0639350:intron 30.0%
TGGAATAATGATTCTTGTCA+TGG - chr4_3:35029455-35029474 Msa0639350:intron 30.0%
TGGATCTGATGATATATAGT+TGG - chr4_3:35029679-35029698 Msa0639350:intron 30.0%
TTTCGTGTGTATAGTATACT+AGG - chr4_3:35029799-35029818 Msa0639350:five_prime_UTR 30.0%
AAAAACACAACCAAGGAACA+AGG - chr4_3:35026044-35026063 Msa0639350:CDS 35.0%
! AGGAGTTGCTATCTTTTTCT+TGG + chr4_3:35026070-35026089 None:intergenic 35.0%
TTGTGTTGGAGAATGGTAAT+TGG + chr4_3:35026230-35026249 None:intergenic 35.0%
!! CGTTGACTTCAATATTGTGT+TGG + chr4_3:35026244-35026263 None:intergenic 35.0%
!! AATACTAAAGAGTGTCTGTG+TGG - chr4_3:35026601-35026620 Msa0639350:CDS 35.0%
TGTCTGTGTGGTACTAATTA+AGG - chr4_3:35026613-35026632 Msa0639350:CDS 35.0%
ATCTCGTGCACTAGTTAAAA+AGG + chr4_3:35026744-35026763 None:intergenic 35.0%
GGTTATTAATCTCACCACTA+TGG - chr4_3:35026912-35026931 Msa0639350:CDS 35.0%
GGTTCAAAATTTAATCCGGT+CGG + chr4_3:35027117-35027136 None:intergenic 35.0%
AGTGATAGTTAACTATCTGG+GGG + chr4_3:35027326-35027345 None:intergenic 35.0%
CACATGTTTGTAGGAAACAT+TGG - chr4_3:35027402-35027421 Msa0639350:intron 35.0%
ATCAATGGTGTGATGACATT+TGG + chr4_3:35027677-35027696 None:intergenic 35.0%
TACAAGCTTGCCATATATGA+TGG - chr4_3:35028092-35028111 Msa0639350:intron 35.0%
AATAAGAGCAAGAGGAATAG+TGG + chr4_3:35028168-35028187 None:intergenic 35.0%
CTTTCGAAAATAAGAGCAAG+AGG + chr4_3:35028176-35028195 None:intergenic 35.0%
!!! CTCTTGCTCTTATTTTCGAA+AGG - chr4_3:35028174-35028193 Msa0639350:intron 35.0%
AATGCAATGCAATGAAACAG+TGG - chr4_3:35028583-35028602 Msa0639350:intron 35.0%
TTAAACATACCCGAAAGACA+CGG + chr4_3:35028710-35028729 None:intergenic 35.0%
!! TGATCGTCTATGTAGTTTTG+AGG - chr4_3:35028811-35028830 Msa0639350:intron 35.0%
ATTAAATTGGCGAGCAGTAA+CGG - chr4_3:35028835-35028854 Msa0639350:intron 35.0%
AAGGTTGTAGGAACTATACT+TGG - chr4_3:35028866-35028885 Msa0639350:intron 35.0%
! GAACTATACTTGGATTTGCT+GGG - chr4_3:35028876-35028895 Msa0639350:intron 35.0%
!! AACTATACTTGGATTTGCTG+GGG - chr4_3:35028877-35028896 Msa0639350:intron 35.0%
! GCCTTTTGCAATCAATCTTT+TGG - chr4_3:35028943-35028962 Msa0639350:intron 35.0%
TAGAACACCTAACAATTCAG+TGG + chr4_3:35029008-35029027 None:intergenic 35.0%
TCAAAGCTATGCTTGATTGA+GGG + chr4_3:35029195-35029214 None:intergenic 35.0%
ATCAAAGCTATGCTTGATTG+AGG + chr4_3:35029196-35029215 None:intergenic 35.0%
GAAGCATACCGAAAAAACAA+CGG + chr4_3:35029314-35029333 None:intergenic 35.0%
TAATGATTCTTGTCATGGCA+TGG - chr4_3:35029460-35029479 Msa0639350:intron 35.0%
AATGATTCTTGTCATGGCAT+GGG - chr4_3:35029461-35029480 Msa0639350:intron 35.0%
TTGAAAGCAGACGCATATAA+AGG + chr4_3:35029504-35029523 None:intergenic 35.0%
!! GCTACAATCAAAAGCATTAG+AGG + chr4_3:35029528-35029547 None:intergenic 35.0%
TTGGATGAGAAGTTAAACCT+CGG - chr4_3:35029567-35029586 Msa0639350:intron 35.0%
! AATTGGTTTCAATCTCTGCA+AGG + chr4_3:35029646-35029665 None:intergenic 35.0%
GATTGTTTGTGGCTTATATG+CGG - chr4_3:35029831-35029850 Msa0639350:five_prime_UTR 35.0%
GAATCACCAATGATTTCTGA+GGG + chr4_3:35029904-35029923 None:intergenic 35.0%
!! CTATTTTCTTGGAGTACCTC+TGG + chr4_3:35025999-35026018 None:intergenic 40.0%
!!! TTTTTCTTGGCCTTGTTCCT+TGG + chr4_3:35026057-35026076 None:intergenic 40.0%
! TCTTTTACTATCCAACCGAC+CGG - chr4_3:35027099-35027118 Msa0639350:intron 40.0%
CAAAATTTAATCCGGTCGGT+TGG + chr4_3:35027113-35027132 None:intergenic 40.0%
!! TCATGCATTAAGTATTGGGC+TGG - chr4_3:35027202-35027221 Msa0639350:CDS 40.0%
!! CATGCATTAAGTATTGGGCT+GGG - chr4_3:35027203-35027222 Msa0639350:CDS 40.0%
CAACGGTAGTTAACTACAGT+CGG + chr4_3:35027366-35027385 None:intergenic 40.0%
GACTGTAGTTAACTACCGTT+GGG - chr4_3:35027365-35027384 Msa0639350:intron 40.0%
! GTTAACTACCGTTGGGTTTT+TGG - chr4_3:35027372-35027391 Msa0639350:intron 40.0%
GAGAATGACCAAAAACCCAA+CGG + chr4_3:35027383-35027402 None:intergenic 40.0%
GGTCATTCTCACATGTTTGT+AGG - chr4_3:35027393-35027412 Msa0639350:intron 40.0%
TCATAGGGTGGGAAAAGTAA+TGG - chr4_3:35027609-35027628 Msa0639350:intron 40.0%
!! AGTTTGACTCGGACTATCAA+TGG + chr4_3:35027692-35027711 None:intergenic 40.0%
TCACGAACGTAAGTTTGACT+CGG + chr4_3:35027703-35027722 None:intergenic 40.0%
ACCATACTTCCCTTCCTTAA+TGG - chr4_3:35027959-35027978 Msa0639350:five_prime_UTR 40.0%
TTGCAGTCTGATCTTGAAGA+TGG - chr4_3:35027992-35028011 Msa0639350:intron 40.0%
TGCAGTCTGATCTTGAAGAT+GGG - chr4_3:35027993-35028012 Msa0639350:intron 40.0%
AACACTCTCCAAGTCATCTT+CGG + chr4_3:35028438-35028457 None:intergenic 40.0%
AAACAAGAGCTCCGTAGAAT+AGG + chr4_3:35028624-35028643 None:intergenic 40.0%
TTAAATTGGCGAGCAGTAAC+GGG - chr4_3:35028836-35028855 Msa0639350:intron 40.0%
GGAACTATACTTGGATTTGC+TGG - chr4_3:35028875-35028894 Msa0639350:intron 40.0%
GATGCTACCACTGAATTGTT+AGG - chr4_3:35028998-35029017 Msa0639350:intron 40.0%
! GTTGCTTCTGTTTCTCAATC+TGG - chr4_3:35029041-35029060 Msa0639350:intron 40.0%
ATACCGAAAAAACAACGGTG+AGG + chr4_3:35029309-35029328 None:intergenic 40.0%
! TTGAAGGGAGTTTTTGCATC+TGG - chr4_3:35029435-35029454 Msa0639350:intron 40.0%
TAACTTCTCATCCAAGAGCA+TGG + chr4_3:35029562-35029581 None:intergenic 40.0%
ATGAGAAGTTAAACCTCGGA+AGG - chr4_3:35029571-35029590 Msa0639350:intron 40.0%
CGTGTGTATAGTATACTAGG+AGG - chr4_3:35029802-35029821 Msa0639350:five_prime_UTR 40.0%
TTAGAGCCCTCAGAAATCAT+TGG - chr4_3:35029895-35029914 Msa0639350:five_prime_UTR 40.0%
GGAATCACCAATGATTTCTG+AGG + chr4_3:35029905-35029924 None:intergenic 40.0%
!! TAAATGAGCATCGATGCTCA+TGG + chr4_3:35029952-35029971 None:intergenic 40.0%
! CAGTAAACACAAAGCACCAG+AGG - chr4_3:35025940-35025959 Msa0639350:exon 45.0%
! TACGTACCGGTAAGTGGATT+GGG + chr4_3:35026105-35026124 None:intergenic 45.0%
! TTACGTACCGGTAAGTGGAT+TGG + chr4_3:35026106-35026125 None:intergenic 45.0%
! CAATGTTACGTACCGGTAAG+TGG + chr4_3:35026111-35026130 None:intergenic 45.0%
CGACTGTAGTTAACTACCGT+TGG - chr4_3:35027364-35027383 Msa0639350:intron 45.0%
CTGTGAGAAGCTCATAGGAT+GGG + chr4_3:35027479-35027498 None:intergenic 45.0%
GCCATTAAGGAAGGGAAGTA+TGG + chr4_3:35027963-35027982 None:intergenic 45.0%
TACTTCCCTTCCTTAATGGC+TGG - chr4_3:35027963-35027982 Msa0639350:five_prime_UTR 45.0%
ACTTCCCTTCCTTAATGGCT+GGG - chr4_3:35027964-35027983 Msa0639350:five_prime_UTR 45.0%
!! CATTAGCAAGGCTGGTTTCA+AGG + chr4_3:35028033-35028052 None:intergenic 45.0%
CTTGAAACCAGCCTTGCTAA+TGG - chr4_3:35028031-35028050 Msa0639350:intron 45.0%
AAACCAGCCTTGCTAATGGT+TGG - chr4_3:35028035-35028054 Msa0639350:intron 45.0%
AACCAGCCTTGCTAATGGTT+GGG - chr4_3:35028036-35028055 Msa0639350:intron 45.0%
AATAGTGGTAACCGCTGCAA+AGG + chr4_3:35028153-35028172 None:intergenic 45.0%
! CAAAAGTGACGGAGGGAATA+AGG + chr4_3:35028681-35028700 None:intergenic 45.0%
TTTGTCTTGGCCGTGTCTTT+CGG - chr4_3:35028697-35028716 Msa0639350:intron 45.0%
! CTCAATCTGGCTCATCATTC+AGG - chr4_3:35029054-35029073 Msa0639350:intron 45.0%
TTGAGAGGGATTTGAACCAG+TGG - chr4_3:35029260-35029279 Msa0639350:intron 45.0%
GATTTGAACCAGTGGAAGCT+TGG - chr4_3:35029268-35029287 Msa0639350:intron 45.0%
!!! GCTCCTCACCGTTGTTTTTT+CGG - chr4_3:35029303-35029322 Msa0639350:intron 45.0%
!! GGAGGAGTGTTGATTGTTTG+TGG - chr4_3:35029820-35029839 Msa0639350:five_prime_UTR 45.0%
!! TGGCTTATATGCGGTGCTAT+GGG - chr4_3:35029840-35029859 Msa0639350:five_prime_UTR 45.0%
GATGCTCATGGACTCAACAA+TGG + chr4_3:35029940-35029959 None:intergenic 45.0%
!! GATTTAAAAATATTTAAATA+CGG + chr4_3:35026987-35027006 None:intergenic 5.0%
!! ACAAAGCACCAGAGGTACTG+AGG - chr4_3:35025948-35025967 Msa0639350:CDS 50.0%
GTGTTTGTCCTCAGTACCTC+TGG + chr4_3:35025959-35025978 None:intergenic 50.0%
TACCTCTGGTACACTAAGCC+TGG + chr4_3:35025985-35026004 None:intergenic 50.0%
ATAGCAACTCCTCGAAGAGC+CGG - chr4_3:35026078-35026097 Msa0639350:intron 50.0%
GGCTGGGCATAAGTCTAGAT+GGG - chr4_3:35027219-35027238 Msa0639350:CDS 50.0%
CCTGTGAGAAGCTCATAGGA+TGG + chr4_3:35027480-35027499 None:intergenic 50.0%
CCATCCTATGAGCTTCTCAC+AGG - chr4_3:35027477-35027496 Msa0639350:intron 50.0%
AGAGCCTGTGAGAAGCTCAT+AGG + chr4_3:35027484-35027503 None:intergenic 50.0%
CTCACAGGCTCTATGCAGTT+CGG - chr4_3:35027492-35027511 Msa0639350:intron 50.0%
GAGCTTCTCGACGTCCTATT+TGG + chr4_3:35027591-35027610 None:intergenic 50.0%
TAGGACGTCGAGAAGCTCAT+AGG - chr4_3:35027593-35027612 Msa0639350:intron 50.0%
AGGACGTCGAGAAGCTCATA+GGG - chr4_3:35027594-35027613 Msa0639350:intron 50.0%
CTTCCCTTCCTTAATGGCTG+GGG - chr4_3:35027965-35027984 Msa0639350:five_prime_UTR 50.0%
AGACCCCAGCCATTAAGGAA+GGG + chr4_3:35027971-35027990 None:intergenic 50.0%
TAGACCCCAGCCATTAAGGA+AGG + chr4_3:35027972-35027991 None:intergenic 50.0%
GCAATAGACCCCAGCCATTA+AGG + chr4_3:35027976-35027995 None:intergenic 50.0%
ACCAGCCTTGCTAATGGTTG+GGG - chr4_3:35028037-35028056 Msa0639350:intron 50.0%
TTGCACCCCAACCATTAGCA+AGG + chr4_3:35028045-35028064 None:intergenic 50.0%
GGAAGAGACCGAAGATGACT+TGG - chr4_3:35028427-35028446 Msa0639350:intron 50.0%
!!! TCCCTCCGTCACTTTTGTCT+TGG - chr4_3:35028684-35028703 Msa0639350:intron 50.0%
! GCCAAGACAAAAGTGACGGA+GGG + chr4_3:35028688-35028707 None:intergenic 50.0%
CACGGCCAAGACAAAAGTGA+CGG + chr4_3:35028692-35028711 None:intergenic 50.0%
TTGTCTTGGCCGTGTCTTTC+GGG - chr4_3:35028698-35028717 Msa0639350:intron 50.0%
! AGCAGTAACGGGAAAGGCAA+AGG - chr4_3:35028847-35028866 Msa0639350:intron 50.0%
ACGGGAAAGGCAAAGGTTGT+AGG - chr4_3:35028854-35028873 Msa0639350:intron 50.0%
!! ACTTGGATTTGCTGGGGCAT+TGG - chr4_3:35028883-35028902 Msa0639350:intron 50.0%
GGATCCAAAGAACGACCACA+CGG - chr4_3:35028964-35028983 Msa0639350:intron 50.0%
GCTATGCTTGATTGAGGGCA+TGG + chr4_3:35029190-35029209 None:intergenic 50.0%
TGAACCAGTGGAAGCTTGGT+TGG - chr4_3:35029272-35029291 Msa0639350:intron 50.0%
GAACCAGTGGAAGCTTGGTT+GGG - chr4_3:35029273-35029292 Msa0639350:intron 50.0%
TATCCCAACCAAGCTTCCAC+TGG + chr4_3:35029279-35029298 None:intergenic 50.0%
GGAAGCTTGGTTGGGATATC+AGG - chr4_3:35029281-35029300 Msa0639350:intron 50.0%
GCATGGGTCGTACAGAAGAA+AGG - chr4_3:35029477-35029496 Msa0639350:intron 50.0%
ATTCGTCGCATCCATGCTCT+TGG - chr4_3:35029548-35029567 Msa0639350:intron 50.0%
! GTGGCTTATATGCGGTGCTA+TGG - chr4_3:35029839-35029858 Msa0639350:five_prime_UTR 50.0%
!! GGCTTATATGCGGTGCTATG+GGG - chr4_3:35029841-35029860 Msa0639350:five_prime_UTR 50.0%
!! TATGCGGTGCTATGGGGTAA+AGG - chr4_3:35029847-35029866 Msa0639350:five_prime_UTR 50.0%
! TTGGTGATTCCGAAGCTGCA+AGG - chr4_3:35029914-35029933 Msa0639350:five_prime_UTR 50.0%
! CAACAATGGCCTTGCAGCTT+CGG + chr4_3:35029926-35029945 None:intergenic 50.0%
!! ACCGGTAAGTGGATTGGGTC+CGG + chr4_3:35026100-35026119 None:intergenic 55.0%
GGGCTGGGCATAAGTCTAGA+TGG - chr4_3:35027218-35027237 Msa0639350:CDS 55.0%
ACGTCGAGAAGCTCATAGGG+TGG - chr4_3:35027597-35027616 Msa0639350:intron 55.0%
CGTCGAGAAGCTCATAGGGT+GGG - chr4_3:35027598-35027617 Msa0639350:intron 55.0%
ACCCCAACCATTAGCAAGGC+TGG + chr4_3:35028041-35028060 None:intergenic 55.0%
CTATCGTCTCGCCTTTGCAG+CGG - chr4_3:35028139-35028158 Msa0639350:intron 55.0%
GGCCAAGACAAAAGTGACGG+AGG + chr4_3:35028689-35028708 None:intergenic 55.0%
! TTGGCGAGCAGTAACGGGAA+AGG - chr4_3:35028841-35028860 Msa0639350:intron 55.0%
CGTTCCGTGTGGTCGTTCTT+TGG + chr4_3:35028971-35028990 None:intergenic 55.0%
CATCAGTGACACGTTCCGTG+TGG + chr4_3:35028982-35029001 None:intergenic 55.0%
ACTGAGGACAAACACGCGCC+AGG - chr4_3:35025964-35025983 Msa0639350:CDS 60.0%
CGCCAGGCTTAGTGTACCAG+AGG - chr4_3:35025980-35025999 Msa0639350:CDS 60.0%
GCCGGACCCAATCCACTTAC+CGG - chr4_3:35026096-35026115 Msa0639350:intron 60.0%
CGCTGCAAAGGCGAGACGAT+AGG + chr4_3:35028141-35028160 None:intergenic 60.0%
GGATTGGGTCCGGCTCTTCG+AGG + chr4_3:35026090-35026109 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 35025919 35029977 35025919 ID=Msa0639350;Name=Msa0639350
chr4_3 mRNA 35025919 35029977 35025919 ID=Msa0639350-mRNA-1;Parent=Msa0639350;Name=Msa0639350-mRNA-1;_AED=0.12;_eAED=0.12;_QI=275|1|1|1|0.85|0.75|8|23|365
chr4_3 exon 35025919 35026083 35025919 ID=Msa0639350-mRNA-1:exon:8260;Parent=Msa0639350-mRNA-1
chr4_3 exon 35026304 35026455 35026304 ID=Msa0639350-mRNA-1:exon:8259;Parent=Msa0639350-mRNA-1
chr4_3 exon 35026572 35026730 35026572 ID=Msa0639350-mRNA-1:exon:8258;Parent=Msa0639350-mRNA-1
chr4_3 exon 35026821 35027070 35026821 ID=Msa0639350-mRNA-1:exon:8257;Parent=Msa0639350-mRNA-1
chr4_3 exon 35027177 35027293 35027177 ID=Msa0639350-mRNA-1:exon:8256;Parent=Msa0639350-mRNA-1
chr4_3 exon 35027409 35027468 35027409 ID=Msa0639350-mRNA-1:exon:8255;Parent=Msa0639350-mRNA-1
chr4_3 exon 35027701 35027994 35027701 ID=Msa0639350-mRNA-1:exon:8254;Parent=Msa0639350-mRNA-1
chr4_3 exon 35029779 35029977 35029779 ID=Msa0639350-mRNA-1:exon:8253;Parent=Msa0639350-mRNA-1
chr4_3 five_prime_UTR 35029779 35029977 35029779 ID=Msa0639350-mRNA-1:five_prime_utr;Parent=Msa0639350-mRNA-1
chr4_3 five_prime_UTR 35027919 35027994 35027919 ID=Msa0639350-mRNA-1:five_prime_utr;Parent=Msa0639350-mRNA-1
chr4_3 CDS 35027701 35027918 35027701 ID=Msa0639350-mRNA-1:cds;Parent=Msa0639350-mRNA-1
chr4_3 CDS 35027409 35027468 35027409 ID=Msa0639350-mRNA-1:cds;Parent=Msa0639350-mRNA-1
chr4_3 CDS 35027177 35027293 35027177 ID=Msa0639350-mRNA-1:cds;Parent=Msa0639350-mRNA-1
chr4_3 CDS 35026821 35027070 35026821 ID=Msa0639350-mRNA-1:cds;Parent=Msa0639350-mRNA-1
chr4_3 CDS 35026572 35026730 35026572 ID=Msa0639350-mRNA-1:cds;Parent=Msa0639350-mRNA-1
chr4_3 CDS 35026304 35026455 35026304 ID=Msa0639350-mRNA-1:cds;Parent=Msa0639350-mRNA-1
chr4_3 CDS 35025942 35026083 35025942 ID=Msa0639350-mRNA-1:cds;Parent=Msa0639350-mRNA-1
chr4_3 three_prime_UTR 35025919 35025941 35025919 ID=Msa0639350-mRNA-1:three_prime_utr;Parent=Msa0639350-mRNA-1
Gene Sequence

>Msa0639350

ATGGCTGGGGTCTATTGCAGTCTGATCTTGAAGATGGGAAGCATGTCAAATTCCTTGAAACCAGCCTTGCTAATGGTTGGGGTGCAAATAGTATTTTCTGCTTGCAATATTATCTACAAGCTTGCCATATATGATGGAATGAGTATCATAGTCATTGCAGCCTATCGTCTCGCCTTTGCAGCGGTTACCACTATTCCTCTTGCTCTTATTTTCGAAAGGAAGAGACCGAAGATGACTTGGAGAGTGTTTTACCTTTCACTTCTAAGTGGATTATTTGCTGGACTTTTATTTCAAAACCTATTCTACGGAGCTCTTGTTTTGACATCAGCAACTCTTGTATCAGCTATCTACAACCTTATTCCCTCCGTCACTTTTGTCTTGGCCGTGTCTTTCGGTTTTGAGGAATTAAATTGGCGAGCAGTAACGGGAAAGGCAAAGGTTGTAGGAACTATACTTGGATTTGCTGGGGCATTGGTGTTGACATTTTACAAAGGAGTAGAATTTGATATTTGGCCTTTTGCAATCAATCTTTTGGATCCAAAGAACGACCACACGGAACGTGTCACTGATGCTACCACTGAATTGTTAGGTGTTCTATGTGTCTTACTTAGTTGCTTCTGTTTCTCAATCTGGCTCATCATTCAGGCTAAGATCAGTGAAGTATATCCATGCCCTCAATCAAGCATAGCTTTGATGTCTGTAATAGGAGCAATACAATGTGTTATTTTTGGTTTCATTGTTGAGAGGGATTTGAACCAGTGGAAGCTTGGTTGGGATATCAGGCTCCTCACCGTTGTTTTTTCGGGAGTTTTTGCATCTGGAATAATGATTCTTGTCATGGCATGGGTCGTACAGAAGAAAGGACCTTTATATGCGTCTGCTTTCAATCCTCTAATGCTTTTGATTGTAGCATTCGTCGCATCCATGCTCTTGGATGAGAAGTTAAACCTCGGAAGTATACTAGGAGGAGTGTTGATTGTTTGTGGCTTATATGCGGTGCTATGGGGTAAAGGTAGAGAAACACATAAGAAGATTGAATTAGAGCCCTCAGAAATCATTGGTGATTCCGAAGCTGCAAGGCCATTGTTGAGTCCATGA
Protein sequence

>Msa0639350

MAGVYCSLILKMGSMSNSLKPALLMVGVQIVFSACNIIYKLAIYDGMSIIVIAAYRLAFAAVTTIPLALIFERKRPKMTWRVFYLSLLSGLFAGLLFQNLFYGALVLTSATLVSAIYNLIPSVTFVLAVSFGFEELNWRAVTGKAKVVGTILGFAGALVLTFYKGVEFDIWPFAINLLDPKNDHTERVTDATTELLGVLCVLLSCFCFSIWLIIQAKISEVYPCPQSSIALMSVIGAIQCVIFGFIVERDLNQWKLGWDIRLLTVVFSGVFASGIMILVMAWVVQKKGPLYASAFNPLMLLIVAFVASMLLDEKLNLGSILGGVLIVCGLYAVLWGKGRETHKKIELEPSEIIGDSEAARPLLSP*