AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0347660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0347660 MtrunA17_Chr3g0080031 97.647 170 4 0 1 170 1 170 4.66e-123 343
Msa0347660 MtrunA17_Chr6g0457591 50.610 164 77 2 9 170 3 164 8.89e-54 168
Msa0347660 MtrunA17_Chr6g0460101 54.887 133 59 1 2 134 25 156 1.16e-47 153
Msa0347660 MtrunA17_Chr6g0457601 58.678 121 49 1 14 134 17 136 5.50e-47 151
Msa0347660 MtrunA17_Chr6g0452621 58.140 129 49 3 9 134 3 129 1.44e-46 148
Msa0347660 MtrunA17_Chr6g0460111 47.692 65 34 0 106 170 1 65 1.56e-16 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0347660 AT5G56170.1 61.702 141 50 2 34 170 28 168 3.02e-60 184
Msa0347660 AT4G26466.1 51.064 141 64 3 34 170 25 164 2.94e-48 154
Msa0347660 AT2G20700.1 54.478 134 61 0 37 170 27 160 9.38e-48 153
Msa0347660 AT4G28280.3 56.061 132 55 1 37 168 10 138 2.02e-46 149
Msa0347660 AT4G28280.2 56.061 132 55 1 37 168 31 159 3.20e-46 149
Msa0347660 AT4G28280.1 65.934 91 31 0 53 143 21 111 3.24e-41 135

Find 42 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGCTACATTAATCTTTA+TGG 0.084552 3_1:-8851816 Msa0347660:CDS
TTTGATGCTGAGACACTTAC+TGG 0.273135 3_1:-8853556 Msa0347660:CDS
TCATTTATCACATCAGTATA+AGG 0.287486 3_1:+8851867 None:intergenic
CTATACGGTAACTATATATA+AGG 0.291152 3_1:+8851484 None:intergenic
TTCTTCACTTCTTCTCATCA+TGG 0.345119 3_1:-8853756 Msa0347660:CDS
AAATGCTCCACAACATTCCT+TGG 0.377041 3_1:+8851905 None:intergenic
TTATTCAACAAATCTATAAC+TGG 0.408997 3_1:-8853856 Msa0347660:five_prime_UTR
GAGTTACTAGGAAGAAAAGT+TGG 0.427866 3_1:+8853710 None:intergenic
CATTCCTTGGGAGGGTACTT+AGG 0.429542 3_1:+8851918 None:intergenic
ATACAACATAGGTAACTATA+CGG 0.442461 3_1:+8851469 None:intergenic
TTAATCATCCATTCCTCAAA+AGG 0.448455 3_1:-8853947 Msa0347660:five_prime_UTR
AACTTAATATTTGACAATTG+AGG 0.454715 3_1:-8853922 Msa0347660:five_prime_UTR
AATGCTCCACAACATTCCTT+GGG 0.478717 3_1:+8851906 None:intergenic
CATTTATCACATCAGTATAA+GGG 0.483862 3_1:+8851868 None:intergenic
TATACGGTAACTATATATAA+GGG 0.487341 3_1:+8851485 None:intergenic
CACTCGCTCGCAAAGAGGCC+AGG 0.497941 3_1:+8851783 None:intergenic
CACGTTCGTTATTGTTGATG+CGG 0.503903 3_1:-8851540 Msa0347660:three_prime_UTR
CACGACACTCGCTCGCAAAG+AGG 0.542743 3_1:+8851778 None:intergenic
ACGTTCGTTATTGTTGATGC+GGG 0.543625 3_1:-8851539 Msa0347660:three_prime_UTR
ACATACTCCATCTCTAGTGC+TGG 0.553326 3_1:-8851682 Msa0347660:CDS
TATTAAAATTAAGAAGATGC+AGG 0.556222 3_1:+8851650 None:intergenic
CGCTCGCAAAGAGGCCAGGA+GGG 0.563412 3_1:+8851787 None:intergenic
GAGCGAGTGTCGTGAAGGGA+AGG 0.564311 3_1:-8851769 Msa0347660:CDS
GATTTGATTTGCAGTATCAT+CGG 0.571086 3_1:+8851707 None:intergenic
ACAACACTCAAATACAACAT+AGG 0.579101 3_1:+8851458 None:intergenic
GTGAGCACCAGCACTAGAGA+TGG 0.582002 3_1:+8851675 None:intergenic
GTATCATCGGCGGACACTGA+TGG 0.584167 3_1:+8851720 None:intergenic
CTTTATGGAAGATACCCTCC+TGG 0.585291 3_1:-8851801 Msa0347660:CDS
TACCCTCCCAAGGAATGTTG+TGG 0.591187 3_1:-8851912 Msa0347660:CDS
ACATCATAGAACAAACATCA+TGG 0.591577 3_1:-8853813 Msa0347660:exon
TTTGCGAGCGAGTGTCGTGA+AGG 0.593755 3_1:-8851774 Msa0347660:CDS
CTCCACAACATTCCTTGGGA+GGG 0.609435 3_1:+8851910 None:intergenic
TTGCGAGCGAGTGTCGTGAA+GGG 0.616291 3_1:-8851773 Msa0347660:CDS
GCTCCACAACATTCCTTGGG+AGG 0.623412 3_1:+8851909 None:intergenic
GAGTGTCGTGAAGGGAAGGA+AGG 0.628790 3_1:-8851765 Msa0347660:CDS
TCGCTCGCAAAGAGGCCAGG+AGG 0.631835 3_1:+8851786 None:intergenic
AAGATTAATGTAGCTAAACA+TGG 0.635506 3_1:+8851821 None:intergenic
ATAATCACAAGCAAATGCAA+AGG 0.641999 3_1:-8851942 Msa0347660:CDS
AATGTAGCTAAACATGGTTG+AGG 0.646232 3_1:+8851827 None:intergenic
TCATCGGCGGACACTGATGG+TGG 0.646590 3_1:+8851723 None:intergenic
AGGACCTAAGTACCCTCCCA+AGG 0.651427 3_1:-8851922 Msa0347660:CDS
TTGATTTGCAGTATCATCGG+CGG 0.696584 3_1:+8851710 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGTTCAAATTTTTATATC+TGG - chr3_1:8851798-8851817 Msa0347660:CDS 15.0%
!!! AAGTTCAAATTTTTATATCT+GGG - chr3_1:8851799-8851818 Msa0347660:CDS 15.0%
!! TAAATTGAAAACAACTTAAT+GGG + chr3_1:8852454-8852473 None:intergenic 15.0%
!! ATAAATTGAAAACAACTTAA+TGG + chr3_1:8852455-8852474 None:intergenic 15.0%
!! AAAAGAAAGAAAAAAGAATT+AGG - chr3_1:8852925-8852944 Msa0347660:intron 15.0%
!!! AGTAATATTTTATGTAGAAT+AGG - chr3_1:8854019-8854038 Msa0347660:five_prime_UTR 15.0%
!!! TTTTTCTTGTCTTTGAAAAA+TGG + chr3_1:8851407-8851426 None:intergenic 20.0%
!!! AATATTAAGTTTTCCTTTTG+AGG + chr3_1:8851476-8851495 None:intergenic 20.0%
!! AACTTAATATTTGACAATTG+AGG - chr3_1:8851485-8851504 Msa0347660:three_prime_UTR 20.0%
!! TTATTCAACAAATCTATAAC+TGG - chr3_1:8851551-8851570 Msa0347660:three_prime_UTR 20.0%
!! ATTATTATTGTTTGTAGTTG+TGG - chr3_1:8852077-8852096 Msa0347660:intron 20.0%
!! TATTTCAGTTATCTATGTTT+AGG - chr3_1:8852997-8853016 Msa0347660:intron 20.0%
!! TTATTGTATAATACATGTAC+TGG - chr3_1:8853326-8853345 Msa0347660:intron 20.0%
!! TTTAGCTACATTAATCTTTA+TGG - chr3_1:8853591-8853610 Msa0347660:intron 20.0%
!! TATTAAAATTAAGAAGATGC+AGG + chr3_1:8853760-8853779 None:intergenic 20.0%
!! TATACGGTAACTATATATAA+GGG + chr3_1:8853925-8853944 None:intergenic 20.0%
!!! TCTTTTTTCTTCTATGCTTT+TGG - chr3_1:8851917-8851936 Msa0347660:CDS 25.0%
!! AGTTTTTCCAATATTTGAAG+TGG - chr3_1:8852192-8852211 Msa0347660:intron 25.0%
!!! GTTTTTCCAATATTTGAAGT+GGG - chr3_1:8852193-8852212 Msa0347660:intron 25.0%
!! GATTTGAAGTCACATAAAAA+TGG + chr3_1:8852264-8852283 None:intergenic 25.0%
! TTTATGTGACTTCAAATCTT+TGG - chr3_1:8852266-8852285 Msa0347660:intron 25.0%
! TAGGATCAAAATGATAACTT+AGG - chr3_1:8852348-8852367 Msa0347660:intron 25.0%
! AGGATCAAAATGATAACTTA+GGG - chr3_1:8852349-8852368 Msa0347660:intron 25.0%
! AGATTGTTTGAGAGAATATA+TGG - chr3_1:8852411-8852430 Msa0347660:intron 25.0%
! TATTTCCATAAGCTCTATAT+AGG - chr3_1:8852472-8852491 Msa0347660:intron 25.0%
! TTACACACATACATTAGTAT+TGG - chr3_1:8852626-8852645 Msa0347660:intron 25.0%
! TACACACATACATTAGTATT+GGG - chr3_1:8852627-8852646 Msa0347660:intron 25.0%
! TGATAATTCAACAAGATACA+TGG + chr3_1:8852727-8852746 None:intergenic 25.0%
! TACACACTATAGTATTTATG+TGG + chr3_1:8852880-8852899 None:intergenic 25.0%
! ATACTATAGTGTGTAGAATT+TGG - chr3_1:8852885-8852904 Msa0347660:intron 25.0%
! CATTTATCACATCAGTATAA+GGG + chr3_1:8853542-8853561 None:intergenic 25.0%
! TCATTTATCACATCAGTATA+AGG + chr3_1:8853543-8853562 None:intergenic 25.0%
! AAGATTAATGTAGCTAAACA+TGG + chr3_1:8853589-8853608 None:intergenic 25.0%
! CTATACGGTAACTATATATA+AGG + chr3_1:8853926-8853945 None:intergenic 25.0%
! ATACAACATAGGTAACTATA+CGG + chr3_1:8853941-8853960 None:intergenic 25.0%
TTAATCATCCATTCCTCAAA+AGG - chr3_1:8851460-8851479 Msa0347660:three_prime_UTR 30.0%
!! TAAGTTTTCCTTTTGAGGAA+TGG + chr3_1:8851471-8851490 None:intergenic 30.0%
! GTTTGTTCTATGATGTTTTG+TGG + chr3_1:8851590-8851609 None:intergenic 30.0%
ACATCATAGAACAAACATCA+TGG - chr3_1:8851594-8851613 Msa0347660:three_prime_UTR 30.0%
AAAAAGTTGTGAAGTGAAGA+AGG + chr3_1:8851729-8851748 None:intergenic 30.0%
!!! CTTTTTTAGCTTGAAGAAGA+TGG + chr3_1:8851880-8851899 None:intergenic 30.0%
CATCTTCTTCAAGCTAAAAA+AGG - chr3_1:8851878-8851897 Msa0347660:CDS 30.0%
TTTAGTCCCACTTCAAATAT+TGG + chr3_1:8852202-8852221 None:intergenic 30.0%
! TATTTGAAGTGGGACTAAAA+TGG - chr3_1:8852203-8852222 Msa0347660:intron 30.0%
TGATCTCACATAAAGCTTAA+TGG + chr3_1:8852234-8852253 None:intergenic 30.0%
TGACTTCAAATCTTTGGATT+TGG - chr3_1:8852272-8852291 Msa0347660:intron 30.0%
CTATACTTTAGACCTCAATT+TGG + chr3_1:8852331-8852350 None:intergenic 30.0%
! CAAATTGAGGTCTAAAGTAT+AGG - chr3_1:8852329-8852348 Msa0347660:intron 30.0%
ATGATAACTTAGGGCTTATT+TGG - chr3_1:8852358-8852377 Msa0347660:intron 30.0%
TGATAACTTAGGGCTTATTT+GGG - chr3_1:8852359-8852378 Msa0347660:intron 30.0%
ACACACATACATTAGTATTG+GGG - chr3_1:8852628-8852647 Msa0347660:intron 30.0%
! ACAGCAATTTTTCCAGAATT+TGG + chr3_1:8852677-8852696 None:intergenic 30.0%
GATAAAATATACCGTCTTTG+TGG - chr3_1:8852698-8852717 Msa0347660:intron 30.0%
GATCTAATCATGTTCAAAGT+TGG + chr3_1:8852795-8852814 None:intergenic 30.0%
TGATTAGATCTTCGTATTCT+TGG - chr3_1:8852805-8852824 Msa0347660:intron 30.0%
AACAGCTATTATCTAGAGAA+CGG + chr3_1:8852833-8852852 None:intergenic 30.0%
! AGAATTAGGCTCTTTCTTTT+TGG - chr3_1:8852939-8852958 Msa0347660:intron 30.0%
ATTCATTACTGAACTCATGT+AGG - chr3_1:8853034-8853053 Msa0347660:intron 30.0%
ACTACAATGCTATAACATAG+TGG - chr3_1:8853122-8853141 Msa0347660:intron 30.0%
ATAATCACAAGCAAATGCAA+AGG - chr3_1:8853465-8853484 Msa0347660:intron 30.0%
!! GATTTGATTTGCAGTATCAT+CGG + chr3_1:8853703-8853722 None:intergenic 30.0%
ACAACACTCAAATACAACAT+AGG + chr3_1:8853952-8853971 None:intergenic 30.0%
!! TTAGAGATTGCAAGTTTTGT+TGG - chr3_1:8853972-8853991 Msa0347660:five_prime_UTR 30.0%
!!! AGTTTTGTTGAGAAGAAGCT+TGG + chr3_1:8851431-8851450 None:intergenic 35.0%
TTCTTCACTTCTTCTCATCA+TGG - chr3_1:8851651-8851670 Msa0347660:CDS 35.0%
GAGTTACTAGGAAGAAAAGT+TGG + chr3_1:8851700-8851719 None:intergenic 35.0%
AGAAGGAAAAGAGAGTTACT+AGG + chr3_1:8851712-8851731 None:intergenic 35.0%
!!! AATTTTGAAGCGCCAAATTG+AGG - chr3_1:8852316-8852335 Msa0347660:intron 35.0%
GCTATCCTATATAGAGCTTA+TGG + chr3_1:8852480-8852499 None:intergenic 35.0%
ACTAATGTATGTGTGTAACC+TGG + chr3_1:8852624-8852643 None:intergenic 35.0%
! ACATACATTAGTATTGGGGA+TGG - chr3_1:8852632-8852651 Msa0347660:intron 35.0%
ATAGCTGTTTGAGAAAGATC+TGG - chr3_1:8852844-8852863 Msa0347660:intron 35.0%
TTGCATTGAATCAATGCAAC+TGG + chr3_1:8852968-8852987 None:intergenic 35.0%
TACACCATGTTGTCAAATAC+AGG - chr3_1:8853186-8853205 Msa0347660:intron 35.0%
ACAACCTGTATTTGACAACA+TGG + chr3_1:8853193-8853212 None:intergenic 35.0%
TTGTCAAATACAGGTTGTAG+CGG - chr3_1:8853195-8853214 Msa0347660:intron 35.0%
!!! ACGTTATTTTTCACAATCCG+CGG - chr3_1:8853255-8853274 Msa0347660:intron 35.0%
! ACTGATCTATAGCAGAGTTT+TGG - chr3_1:8853286-8853305 Msa0347660:intron 35.0%
AATGTAGCTAAACATGGTTG+AGG + chr3_1:8853583-8853602 None:intergenic 35.0%
TTTGATGCTGAGACACTTAC+TGG - chr3_1:8851851-8851870 Msa0347660:CDS 40.0%
AAGTTTATCGAAACACAGCC+AGG - chr3_1:8852603-8852622 Msa0347660:intron 40.0%
!! GGATGGACTATAGAGAAGTA+TGG - chr3_1:8852649-8852668 Msa0347660:intron 40.0%
! AGAAGTATGGCTCCAAATTC+TGG - chr3_1:8852662-8852681 Msa0347660:intron 40.0%
ATACATGGAAGCCACAAAGA+CGG + chr3_1:8852712-8852731 None:intergenic 40.0%
TGTCAAATACAGGTTGTAGC+GGG - chr3_1:8853196-8853215 Msa0347660:intron 40.0%
!!! ATAGCAGAGTTTTGGTTGTG+TGG - chr3_1:8853294-8853313 Msa0347660:intron 40.0%
! ATCAGCAAACGCTTCTCTTT+TGG + chr3_1:8853384-8853403 None:intergenic 40.0%
AATGCTCCACAACATTCCTT+GGG + chr3_1:8853504-8853523 None:intergenic 40.0%
AAATGCTCCACAACATTCCT+TGG + chr3_1:8853505-8853524 None:intergenic 40.0%
!! TTGATTTGCAGTATCATCGG+CGG + chr3_1:8853700-8853719 None:intergenic 40.0%
CACGTTCGTTATTGTTGATG+CGG - chr3_1:8853867-8853886 Msa0347660:five_prime_UTR 40.0%
ACGTTCGTTATTGTTGATGC+GGG - chr3_1:8853868-8853887 Msa0347660:five_prime_UTR 40.0%
ACTATGACACTGTTGCGTAG+CGG - chr3_1:8853220-8853239 Msa0347660:intron 45.0%
! TTTTCACAATCCGCGGTTGA+CGG - chr3_1:8853262-8853281 Msa0347660:intron 45.0%
!! CATGTACTGGTTTGTGTGTG+TGG - chr3_1:8853339-8853358 Msa0347660:intron 45.0%
TTGTTTCACATTGCACCTGC+AGG - chr3_1:8853408-8853427 Msa0347660:intron 45.0%
AAATTCACAGAACAGCCTGC+AGG + chr3_1:8853426-8853445 None:intergenic 45.0%
CTTTATGGAAGATACCCTCC+TGG - chr3_1:8853606-8853625 Msa0347660:intron 45.0%
ACATACTCCATCTCTAGTGC+TGG - chr3_1:8853725-8853744 Msa0347660:CDS 45.0%
! GTGTTGTTACATAGCCGCTG+TGG - chr3_1:8853095-8853114 Msa0347660:intron 50.0%
!! CATTCCTTGGGAGGGTACTT+AGG + chr3_1:8853492-8853511 None:intergenic 50.0%
TACCCTCCCAAGGAATGTTG+TGG - chr3_1:8853495-8853514 Msa0347660:intron 50.0%
CTCCACAACATTCCTTGGGA+GGG + chr3_1:8853500-8853519 None:intergenic 50.0%
GCATTGTAGTGCTGCCACAG+CGG + chr3_1:8853112-8853131 None:intergenic 55.0%
TAGATCAGTGCCGTCAACCG+CGG + chr3_1:8853275-8853294 None:intergenic 55.0%
AGGACCTAAGTACCCTCCCA+AGG - chr3_1:8853485-8853504 Msa0347660:intron 55.0%
GCTCCACAACATTCCTTGGG+AGG + chr3_1:8853501-8853520 None:intergenic 55.0%
! TTTGCGAGCGAGTGTCGTGA+AGG - chr3_1:8853633-8853652 Msa0347660:intron 55.0%
! TTGCGAGCGAGTGTCGTGAA+GGG - chr3_1:8853634-8853653 Msa0347660:intron 55.0%
! GAGTGTCGTGAAGGGAAGGA+AGG - chr3_1:8853642-8853661 Msa0347660:intron 55.0%
GTATCATCGGCGGACACTGA+TGG + chr3_1:8853690-8853709 None:intergenic 55.0%
GTGAGCACCAGCACTAGAGA+TGG + chr3_1:8853735-8853754 None:intergenic 55.0%
CACGACACTCGCTCGCAAAG+AGG + chr3_1:8853632-8853651 None:intergenic 60.0%
! GAGCGAGTGTCGTGAAGGGA+AGG - chr3_1:8853638-8853657 Msa0347660:intron 60.0%
TCATCGGCGGACACTGATGG+TGG + chr3_1:8853687-8853706 None:intergenic 60.0%
CGCTCGCAAAGAGGCCAGGA+GGG + chr3_1:8853623-8853642 None:intergenic 65.0%
TCGCTCGCAAAGAGGCCAGG+AGG + chr3_1:8853624-8853643 None:intergenic 65.0%
CACTCGCTCGCAAAGAGGCC+AGG + chr3_1:8853627-8853646 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 8851387 8854042 8851387 ID=Msa0347660;Name=Msa0347660
chr3_1 mRNA 8851387 8854042 8851387 ID=Msa0347660-mRNA-1;Parent=Msa0347660;Name=Msa0347660-mRNA-1;_AED=0.03;_eAED=0.03;_QI=226|1|1|1|1|1|3|257|170
chr3_1 exon 8851387 8851999 8851387 ID=Msa0347660-mRNA-1:exon:2557;Parent=Msa0347660-mRNA-1
chr3_1 exon 8853530 8853586 8853530 ID=Msa0347660-mRNA-1:exon:2556;Parent=Msa0347660-mRNA-1
chr3_1 exon 8853717 8854042 8853717 ID=Msa0347660-mRNA-1:exon:2555;Parent=Msa0347660-mRNA-1
chr3_1 five_prime_UTR 8853817 8854042 8853817 ID=Msa0347660-mRNA-1:five_prime_utr;Parent=Msa0347660-mRNA-1
chr3_1 CDS 8853717 8853816 8853717 ID=Msa0347660-mRNA-1:cds;Parent=Msa0347660-mRNA-1
chr3_1 CDS 8853530 8853586 8853530 ID=Msa0347660-mRNA-1:cds;Parent=Msa0347660-mRNA-1
chr3_1 CDS 8851644 8851999 8851644 ID=Msa0347660-mRNA-1:cds;Parent=Msa0347660-mRNA-1
chr3_1 three_prime_UTR 8851387 8851643 8851387 ID=Msa0347660-mRNA-1:three_prime_utr;Parent=Msa0347660-mRNA-1
Gene Sequence

>Msa0347660

ATGGCTTGTTCTATGAATCAACAAAGCTTCTTGCTTTGTTCTTCACTTCTTCTCATCATGGCTGTTTCTGTTTCTTCTTTTCATCCAACTTTTCTTCCTAATACTATTTTTGATGCTGAGACACTTACTGGTCGCCATCTTCTTCAAGCTAAAAAAGGCTGTTCTGTGAATTTTGAGTTTCTAAACTATACAATAATCACAAGCAAATGCAAAGGACCTAAGTACCCTCCCAAGGAATGTTGTGGAGCATTTAAAGAGTTTGCTTGCCCTTATACTGATGTGATAAATGATTTAACAAACGATTGTGCCTCAACCATGTTTAGCTACATTAATCTTTATGGAAGATACCCTCCTGGCCTCTTTGCGAGCGAGTGTCGTGAAGGGAAGGAAGGTCTAGCATGTGATGCATTGCCACCATCAGTGTCCGCCGATGATACTGCAAATCAAATCGTACATACTCCATCTCTAGTGCTGGTGCTCACAGCCTGCATCTTCTTAATTTTAATATTCTGA

Protein sequence

>Msa0347660

MACSMNQQSFLLCSSLLLIMAVSVSSFHPTFLPNTIFDAETLTGRHLLQAKKGCSVNFEFLNYTIITSKCKGPKYPPKECCGAFKEFACPYTDVINDLTNDCASTMFSYINLYGRYPPGLFASECREGKEGLACDALPPSVSADDTANQIVHTPSLVLVLTACIFLILIF*