Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1373070 | XP_003626248.1 | 99.206 | 126 | 1 | 0 | 1 | 126 | 1 | 126 | 1.06e-88 | 263 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1373070 | sp|Q8L9P8|RLF33_ARATH | 59.483 | 116 | 38 | 1 | 11 | 126 | 9 | 115 | 9.08e-43 | 139 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1373070 | A2Q327 | 99.206 | 126 | 1 | 0 | 1 | 126 | 1 | 126 | 5.07e-89 | 263 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1352590 | Msa1373070 | 0.992829 | 1.061299e-195 | -8.615850e-47 |
| Msa1373070 | Msa1442940 | 0.817265 | 3.606895e-52 | -8.615850e-47 |
| Msa0644830 | Msa1373070 | 0.827032 | 1.956528e-54 | -8.615850e-47 |
| Msa0649460 | Msa1373070 | 0.830040 | 3.673212e-55 | -8.615850e-47 |
| Msa0713910 | Msa1373070 | 0.808978 | 2.374198e-50 | -8.615850e-47 |
| Msa0756170 | Msa1373070 | 0.815215 | 1.036255e-51 | -8.615850e-47 |
| Msa0206440 | Msa1373070 | 0.837456 | 5.144375e-57 | -8.615850e-47 |
| Msa1268530 | Msa1373070 | 0.802172 | 6.363935e-49 | -8.615850e-47 |
| Msa0467570 | Msa1373070 | 0.835997 | 1.211018e-56 | -8.615850e-47 |
| Msa0249360 | Msa1373070 | 0.826346 | 2.853593e-54 | -8.615850e-47 |
| Msa0287850 | Msa1373070 | 0.835043 | 2.110708e-56 | -8.615850e-47 |
| Msa0514460 | Msa1373070 | 0.816053 | 6.743825e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1373070 | MtrunA17_Chr7g0272541 | 99.206 | 126 | 1 | 0 | 1 | 126 | 1 | 126 | 9.77e-93 | 263 |
| Msa1373070 | MtrunA17_Chr4g0067331 | 57.025 | 121 | 45 | 2 | 9 | 126 | 9 | 125 | 1.07e-41 | 134 |
| Msa1373070 | MtrunA17_Chr7g0269131 | 53.390 | 118 | 49 | 2 | 10 | 127 | 6 | 117 | 6.46e-37 | 122 |
| Msa1373070 | MtrunA17_Chr3g0121871 | 42.735 | 117 | 55 | 5 | 10 | 125 | 21 | 126 | 5.77e-21 | 81.6 |
| Msa1373070 | MtrunA17_Chr4g0047131 | 56.250 | 64 | 26 | 1 | 64 | 125 | 44 | 107 | 1.29e-19 | 77.8 |
| Msa1373070 | MtrunA17_Chr6g0476591 | 48.485 | 66 | 29 | 1 | 60 | 125 | 41 | 101 | 1.17e-15 | 67.4 |
| Msa1373070 | MtrunA17_Chr8g0378021 | 39.080 | 87 | 46 | 3 | 44 | 126 | 33 | 116 | 8.33e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1373070 | AT4G15800.1 | 59.483 | 116 | 38 | 1 | 11 | 126 | 9 | 115 | 9.23e-44 | 139 |
| Msa1373070 | AT1G02900.1 | 58.333 | 120 | 43 | 3 | 9 | 127 | 7 | 120 | 3.42e-42 | 135 |
| Msa1373070 | AT3G16570.3 | 65.556 | 90 | 17 | 2 | 51 | 126 | 47 | 136 | 2.23e-33 | 114 |
| Msa1373070 | AT3G16570.2 | 65.556 | 90 | 17 | 2 | 51 | 126 | 47 | 136 | 2.23e-33 | 114 |
| Msa1373070 | AT3G16570.1 | 65.556 | 90 | 17 | 2 | 51 | 126 | 47 | 136 | 2.23e-33 | 114 |
| Msa1373070 | AT3G05490.1 | 81.034 | 58 | 11 | 0 | 69 | 126 | 61 | 118 | 3.04e-30 | 105 |
| Msa1373070 | AT2G33775.1 | 57.971 | 69 | 28 | 1 | 58 | 125 | 37 | 105 | 4.16e-23 | 87.0 |
| Msa1373070 | AT1G28270.1 | 55.072 | 69 | 30 | 1 | 58 | 125 | 37 | 105 | 1.16e-19 | 78.2 |
| Msa1373070 | AT4G13950.1 | 57.812 | 64 | 25 | 2 | 63 | 125 | 42 | 104 | 3.85e-18 | 74.3 |
| Msa1373070 | AT3G23805.1 | 60.714 | 56 | 20 | 2 | 71 | 125 | 55 | 109 | 1.57e-15 | 67.8 |
| Msa1373070 | AT5G67070.1 | 52.632 | 76 | 25 | 5 | 62 | 126 | 53 | 128 | 3.14e-12 | 59.7 |
Find 32 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCATCGTTGTCATCAAA+AGG | 0.415483 | tig0019363:+2728 | None:intergenic |
| GCACTGCATCCACGGCTGTA+AGG | 0.445471 | tig0019363:+2436 | None:intergenic |
| AGTTTGATAATGAAATCAAC+AGG | 0.461525 | tig0019363:-2573 | Msa1373070:CDS |
| CGCACCATAGCTGATGTACT+TGG | 0.465658 | tig0019363:+2534 | None:intergenic |
| CACTGCATCCACGGCTGTAA+GGG | 0.481328 | tig0019363:+2437 | None:intergenic |
| CAATTGCAGACCCGGTGCTC+AGG | 0.488139 | tig0019363:-2464 | Msa1373070:CDS |
| CCTCCAGCTGCATCTACTGT+TGG | 0.503199 | tig0019363:+2697 | None:intergenic |
| GCAGCTGGAGGATTCGAACT+TGG | 0.512453 | tig0019363:-2685 | Msa1373070:CDS |
| GAAGCACCACGGCGAGAACA+AGG | 0.526826 | tig0019363:+2493 | None:intergenic |
| GTAAGGGTTAGCCTGAGCAC+CGG | 0.526971 | tig0019363:+2453 | None:intergenic |
| ATCAGCGATGGAACCTTCGC+AGG | 0.533132 | tig0019363:+2627 | None:intergenic |
| TCTCCAACAGTAGATGCAGC+TGG | 0.538924 | tig0019363:-2700 | Msa1373070:CDS |
| TCTTACTACAATTGCAGACC+CGG | 0.540294 | tig0019363:-2472 | Msa1373070:CDS |
| AGGATTCGAACTTGGAGGAA+TGG | 0.547500 | tig0019363:-2677 | Msa1373070:CDS |
| GCTGGAGGATTCGAACTTGG+AGG | 0.550332 | tig0019363:-2682 | Msa1373070:CDS |
| TCGAACTTGGAGGAATGGAA+TGG | 0.552877 | tig0019363:-2672 | Msa1373070:CDS |
| CATCATCGTTGTCATCAAAA+GGG | 0.554937 | tig0019363:+2729 | None:intergenic |
| TCAGCTATGGTGCGCTTCAG+AGG | 0.563424 | tig0019363:-2525 | Msa1373070:CDS |
| GCAGTGCTATTACGAGGTGC+AGG | 0.566649 | tig0019363:-2420 | Msa1373070:CDS |
| TCGTAATAGCACTGCATCCA+CGG | 0.577260 | tig0019363:+2428 | None:intergenic |
| TAAGGGTTAGCCTGAGCACC+GGG | 0.580885 | tig0019363:+2454 | None:intergenic |
| TTGTAACATGCAATCAGCGA+TGG | 0.586357 | tig0019363:+2615 | None:intergenic |
| CAGGCTAACCCTTACAGCCG+TGG | 0.595858 | tig0019363:-2445 | Msa1373070:CDS |
| ACGACCAAGTACATCAGCTA+TGG | 0.604031 | tig0019363:-2538 | Msa1373070:CDS |
| AGCGATGGAACCTTCGCAGG+TGG | 0.625016 | tig0019363:+2630 | None:intergenic |
| AAGCACCACGGCGAGAACAA+GGG | 0.626487 | tig0019363:+2494 | None:intergenic |
| ACTGTCCCTTGTTCTCGCCG+TGG | 0.647098 | tig0019363:-2499 | Msa1373070:CDS |
| CCAACAGTAGATGCAGCTGG+AGG | 0.701563 | tig0019363:-2697 | Msa1373070:CDS |
| GCTGATTGCATGTTACAACA+AGG | 0.712911 | tig0019363:-2610 | Msa1373070:CDS |
| GTGGATGCAGTGCTATTACG+AGG | 0.733772 | tig0019363:-2426 | Msa1373070:CDS |
| AAAGGGTGACACAAATCACG+AGG | 0.746825 | tig0019363:+2746 | None:intergenic |
| AATTGTAGTAAGAAGCACCA+CGG | 0.751980 | tig0019363:+2482 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTGATAATGAAATCAAC+AGG | - | tig0019363:2616-2635 | Msa1373070:CDS | 25.0% |
| CATCATCGTTGTCATCAAAA+GGG | + | tig0019363:2463-2482 | None:intergenic | 35.0% | |
| TCATCATCGTTGTCATCAAA+AGG | + | tig0019363:2464-2483 | None:intergenic | 35.0% | |
| !! | AATTGTAGTAAGAAGCACCA+CGG | + | tig0019363:2710-2729 | None:intergenic | 35.0% |
| TTGTAACATGCAATCAGCGA+TGG | + | tig0019363:2577-2596 | None:intergenic | 40.0% | |
| GCTGATTGCATGTTACAACA+AGG | - | tig0019363:2579-2598 | Msa1373070:CDS | 40.0% | |
| TCTTACTACAATTGCAGACC+CGG | - | tig0019363:2717-2736 | Msa1373070:CDS | 40.0% | |
| AAAGGGTGACACAAATCACG+AGG | + | tig0019363:2446-2465 | None:intergenic | 45.0% | |
| AGGATTCGAACTTGGAGGAA+TGG | - | tig0019363:2512-2531 | Msa1373070:CDS | 45.0% | |
| TCGAACTTGGAGGAATGGAA+TGG | - | tig0019363:2517-2536 | Msa1373070:CDS | 45.0% | |
| ACGACCAAGTACATCAGCTA+TGG | - | tig0019363:2651-2670 | Msa1373070:CDS | 45.0% | |
| TCGTAATAGCACTGCATCCA+CGG | + | tig0019363:2764-2783 | None:intergenic | 45.0% | |
| TCTCCAACAGTAGATGCAGC+TGG | - | tig0019363:2489-2508 | Msa1373070:CDS | 50.0% | |
| ACAAAAACAGCCACCTGCGA+AGG | - | tig0019363:2549-2568 | Msa1373070:CDS | 50.0% | |
| CGCACCATAGCTGATGTACT+TGG | + | tig0019363:2658-2677 | None:intergenic | 50.0% | |
| ! | GTGGATGCAGTGCTATTACG+AGG | - | tig0019363:2763-2782 | Msa1373070:CDS | 50.0% |
| CCTCCAGCTGCATCTACTGT+TGG | + | tig0019363:2495-2514 | None:intergenic | 55.0% | |
| CCAACAGTAGATGCAGCTGG+AGG | - | tig0019363:2492-2511 | Msa1373070:CDS | 55.0% | |
| GCAGCTGGAGGATTCGAACT+TGG | - | tig0019363:2504-2523 | Msa1373070:CDS | 55.0% | |
| GCTGGAGGATTCGAACTTGG+AGG | - | tig0019363:2507-2526 | Msa1373070:CDS | 55.0% | |
| ATCAGCGATGGAACCTTCGC+AGG | + | tig0019363:2565-2584 | None:intergenic | 55.0% | |
| TCAGCTATGGTGCGCTTCAG+AGG | - | tig0019363:2664-2683 | Msa1373070:CDS | 55.0% | |
| AAGCACCACGGCGAGAACAA+GGG | + | tig0019363:2698-2717 | None:intergenic | 55.0% | |
| !! | TAAGGGTTAGCCTGAGCACC+GGG | + | tig0019363:2738-2757 | None:intergenic | 55.0% |
| !! | GTAAGGGTTAGCCTGAGCAC+CGG | + | tig0019363:2739-2758 | None:intergenic | 55.0% |
| CACTGCATCCACGGCTGTAA+GGG | + | tig0019363:2755-2774 | None:intergenic | 55.0% | |
| ! | GCAGTGCTATTACGAGGTGC+AGG | - | tig0019363:2769-2788 | Msa1373070:CDS | 55.0% |
| AGCGATGGAACCTTCGCAGG+TGG | + | tig0019363:2562-2581 | None:intergenic | 60.0% | |
| ACTGTCCCTTGTTCTCGCCG+TGG | - | tig0019363:2690-2709 | Msa1373070:CDS | 60.0% | |
| ! | GAAGCACCACGGCGAGAACA+AGG | + | tig0019363:2699-2718 | None:intergenic | 60.0% |
| CAATTGCAGACCCGGTGCTC+AGG | - | tig0019363:2725-2744 | Msa1373070:CDS | 60.0% | |
| CAGGCTAACCCTTACAGCCG+TGG | - | tig0019363:2744-2763 | Msa1373070:CDS | 60.0% | |
| GCACTGCATCCACGGCTGTA+AGG | + | tig0019363:2756-2775 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0019363 | gene | 2414 | 2797 | 2414 | ID=Msa1373070;Name=Msa1373070 |
| tig0019363 | mRNA | 2414 | 2797 | 2414 | ID=Msa1373070-mRNA-1;Parent=Msa1373070;Name=Msa1373070-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|127 |
| tig0019363 | exon | 2414 | 2797 | 2414 | ID=Msa1373070-mRNA-1:exon:3112;Parent=Msa1373070-mRNA-1 |
| tig0019363 | CDS | 2414 | 2797 | 2414 | ID=Msa1373070-mRNA-1:cds;Parent=Msa1373070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
