AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0442260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0442260 MtrunA17_Chr3g0080031 97.647 170 4 0 1 170 1 170 4.66e-123 343
Msa0442260 MtrunA17_Chr6g0457591 50.610 164 77 2 9 170 3 164 8.89e-54 168
Msa0442260 MtrunA17_Chr6g0460101 54.887 133 59 1 2 134 25 156 1.16e-47 153
Msa0442260 MtrunA17_Chr6g0457601 58.678 121 49 1 14 134 17 136 5.50e-47 151
Msa0442260 MtrunA17_Chr6g0452621 58.140 129 49 3 9 134 3 129 1.44e-46 148
Msa0442260 MtrunA17_Chr6g0460111 47.692 65 34 0 106 170 1 65 1.56e-16 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0442260 AT5G56170.1 61.702 141 50 2 34 170 28 168 3.02e-60 184
Msa0442260 AT4G26466.1 51.064 141 64 3 34 170 25 164 2.94e-48 154
Msa0442260 AT2G20700.1 54.478 134 61 0 37 170 27 160 9.38e-48 153
Msa0442260 AT4G28280.3 56.061 132 55 1 37 168 10 138 2.02e-46 149
Msa0442260 AT4G28280.2 56.061 132 55 1 37 168 31 159 3.20e-46 149
Msa0442260 AT4G28280.1 65.934 91 31 0 53 143 21 111 3.24e-41 135

Find 42 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGCTACATTAATCTTTA+TGG 0.084552 3_3:-11729597 Msa0442260:CDS
TTTGATGCTGAGACACTTAC+TGG 0.273135 3_3:-11731336 Msa0442260:CDS
TCATTTATCACATCAGTATA+AGG 0.287486 3_3:+11729648 None:intergenic
CTATACGGTAACTATATATA+AGG 0.291152 3_3:+11729265 None:intergenic
TTCTTCACTTCTTCTCATCA+TGG 0.345119 3_3:-11731531 Msa0442260:CDS
AAATGCTCCACAACATTCCT+TGG 0.377041 3_3:+11729686 None:intergenic
TTATTCAACAAATCTATAAC+TGG 0.408997 3_3:-11731631 Msa0442260:five_prime_UTR
GAGTTACTAGGAAGAAAAGT+TGG 0.427866 3_3:+11731485 None:intergenic
CATTCCTTGGGAGGGTACTT+AGG 0.429542 3_3:+11729699 None:intergenic
ATACAACATAGGTAACTATA+CGG 0.442461 3_3:+11729250 None:intergenic
TTAATCATCCATTCCTCAAA+AGG 0.448455 3_3:-11731722 Msa0442260:five_prime_UTR
AACTTAATATTTGACAATTG+AGG 0.454715 3_3:-11731697 Msa0442260:five_prime_UTR
AATGCTCCACAACATTCCTT+GGG 0.478717 3_3:+11729687 None:intergenic
CATTTATCACATCAGTATAA+GGG 0.483862 3_3:+11729649 None:intergenic
TATACGGTAACTATATATAA+GGG 0.487341 3_3:+11729266 None:intergenic
CACTCGCTCGCAAAGAGGCC+AGG 0.497941 3_3:+11729564 None:intergenic
CACGTTCGTTATTGTTGATG+CGG 0.503903 3_3:-11729321 Msa0442260:three_prime_UTR
CACGACACTCGCTCGCAAAG+AGG 0.542743 3_3:+11729559 None:intergenic
ACGTTCGTTATTGTTGATGC+GGG 0.543625 3_3:-11729320 Msa0442260:three_prime_UTR
ACATACTCCATCTCTAGTGC+TGG 0.553326 3_3:-11729463 Msa0442260:CDS
TATTAAAATTAAGAAGATGC+AGG 0.556222 3_3:+11729431 None:intergenic
CGCTCGCAAAGAGGCCAGGA+GGG 0.563412 3_3:+11729568 None:intergenic
GAGCGAGTGTCGTGAAGGGA+AGG 0.564311 3_3:-11729550 Msa0442260:CDS
GATTTGATTTGCAGTATCAT+CGG 0.571086 3_3:+11729488 None:intergenic
ACAACACTCAAATACAACAT+AGG 0.579101 3_3:+11729239 None:intergenic
GTGAGCACCAGCACTAGAGA+TGG 0.582002 3_3:+11729456 None:intergenic
GTATCATCGGCGGACACTGA+TGG 0.584167 3_3:+11729501 None:intergenic
CTTTATGGAAGATACCCTCC+TGG 0.585291 3_3:-11729582 Msa0442260:CDS
TACCCTCCCAAGGAATGTTG+TGG 0.591187 3_3:-11729693 Msa0442260:CDS
ACATCATAGAACAAACATCA+TGG 0.591577 3_3:-11731588 Msa0442260:exon
TTTGCGAGCGAGTGTCGTGA+AGG 0.593755 3_3:-11729555 Msa0442260:CDS
CTCCACAACATTCCTTGGGA+GGG 0.609435 3_3:+11729691 None:intergenic
TTGCGAGCGAGTGTCGTGAA+GGG 0.616291 3_3:-11729554 Msa0442260:CDS
GCTCCACAACATTCCTTGGG+AGG 0.623412 3_3:+11729690 None:intergenic
GAGTGTCGTGAAGGGAAGGA+AGG 0.628790 3_3:-11729546 Msa0442260:CDS
TCGCTCGCAAAGAGGCCAGG+AGG 0.631835 3_3:+11729567 None:intergenic
AAGATTAATGTAGCTAAACA+TGG 0.635506 3_3:+11729602 None:intergenic
ATAATCACAAGCAAATGCAA+AGG 0.641999 3_3:-11729723 Msa0442260:CDS
AATGTAGCTAAACATGGTTG+AGG 0.646232 3_3:+11729608 None:intergenic
TCATCGGCGGACACTGATGG+TGG 0.646590 3_3:+11729504 None:intergenic
AGGACCTAAGTACCCTCCCA+AGG 0.651427 3_3:-11729703 Msa0442260:CDS
TTGATTTGCAGTATCATCGG+CGG 0.696584 3_3:+11729491 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGTTCAAATTTTTATATC+TGG - chr3_3:11729574-11729593 Msa0442260:CDS 15.0%
!!! AAGTTCAAATTTTTATATCT+GGG - chr3_3:11729575-11729594 Msa0442260:CDS 15.0%
!! TAAATTGAAAACAACTTAAT+GGG + chr3_3:11730229-11730248 None:intergenic 15.0%
!! ATAAATTGAAAACAACTTAA+TGG + chr3_3:11730230-11730249 None:intergenic 15.0%
!! AAAAAAGAGAAAAAAGAATT+AGG - chr3_3:11730700-11730719 Msa0442260:intron 15.0%
!!! AGTAATATTTTATGTAGAAT+AGG - chr3_3:11731794-11731813 Msa0442260:five_prime_UTR 15.0%
!!! TTTTTCTTGTCTTTGAAAAA+TGG + chr3_3:11729188-11729207 None:intergenic 20.0%
!!! AATATTAAGTTTTCCTTTTG+AGG + chr3_3:11729257-11729276 None:intergenic 20.0%
!! AACTTAATATTTGACAATTG+AGG - chr3_3:11729266-11729285 Msa0442260:three_prime_UTR 20.0%
!! TTATTCAACAAATCTATAAC+TGG - chr3_3:11729332-11729351 Msa0442260:three_prime_UTR 20.0%
!! ATTATTATTGTTTGTAGTTG+TGG - chr3_3:11729852-11729871 Msa0442260:intron 20.0%
!! TATTTCAGTTATCTATGTTT+AGG - chr3_3:11730772-11730791 Msa0442260:intron 20.0%
!! TTATTGTATAATACATGTAC+TGG - chr3_3:11731101-11731120 Msa0442260:intron 20.0%
!! TTTAGCTACATTAATCTTTA+TGG - chr3_3:11731366-11731385 Msa0442260:intron 20.0%
!! TATTAAAATTAAGAAGATGC+AGG + chr3_3:11731535-11731554 None:intergenic 20.0%
!! TATACGGTAACTATATATAA+GGG + chr3_3:11731700-11731719 None:intergenic 20.0%
!!! TCTTTTTTCTTCTATGCTTT+TGG - chr3_3:11729693-11729712 Msa0442260:CDS 25.0%
!! AGTTTTTCCAATATTTGAAG+TGG - chr3_3:11729967-11729986 Msa0442260:intron 25.0%
!!! GTTTTTCCAATATTTGAAGT+GGG - chr3_3:11729968-11729987 Msa0442260:intron 25.0%
!! GATTTGAAGTCACATAAAAA+TGG + chr3_3:11730039-11730058 None:intergenic 25.0%
! TTTATGTGACTTCAAATCTT+TGG - chr3_3:11730041-11730060 Msa0442260:intron 25.0%
! TAGGATCAAAATGATAACTT+AGG - chr3_3:11730123-11730142 Msa0442260:intron 25.0%
! AGGATCAAAATGATAACTTA+GGG - chr3_3:11730124-11730143 Msa0442260:intron 25.0%
! AGATTGTTTGAGAGAATATA+TGG - chr3_3:11730186-11730205 Msa0442260:intron 25.0%
! TATTTCCATAAGCTCTATAT+AGG - chr3_3:11730247-11730266 Msa0442260:intron 25.0%
! TTACACACATACATTAGTAT+TGG - chr3_3:11730401-11730420 Msa0442260:intron 25.0%
! TACACACATACATTAGTATT+GGG - chr3_3:11730402-11730421 Msa0442260:intron 25.0%
! TGATAATTCAACAAGATACA+TGG + chr3_3:11730503-11730522 None:intergenic 25.0%
! TACACACTATAGTATTTATG+TGG + chr3_3:11730656-11730675 None:intergenic 25.0%
! ATACTATAGTGTGTAGAATT+TGG - chr3_3:11730661-11730680 Msa0442260:intron 25.0%
! CATTTATCACATCAGTATAA+GGG + chr3_3:11731317-11731336 None:intergenic 25.0%
! TCATTTATCACATCAGTATA+AGG + chr3_3:11731318-11731337 None:intergenic 25.0%
! AAGATTAATGTAGCTAAACA+TGG + chr3_3:11731364-11731383 None:intergenic 25.0%
! CTATACGGTAACTATATATA+AGG + chr3_3:11731701-11731720 None:intergenic 25.0%
! ATACAACATAGGTAACTATA+CGG + chr3_3:11731716-11731735 None:intergenic 25.0%
TTAATCATCCATTCCTCAAA+AGG - chr3_3:11729241-11729260 Msa0442260:three_prime_UTR 30.0%
!! TAAGTTTTCCTTTTGAGGAA+TGG + chr3_3:11729252-11729271 None:intergenic 30.0%
! GTTTGTTCTATGATGTTTTG+TGG + chr3_3:11729371-11729390 None:intergenic 30.0%
ACATCATAGAACAAACATCA+TGG - chr3_3:11729375-11729394 Msa0442260:three_prime_UTR 30.0%
AAAAAGTTGTGAAGTGAAGA+AGG + chr3_3:11729510-11729529 None:intergenic 30.0%
!!! CTTTTTTAGCTTGAAGAAGA+TGG + chr3_3:11729656-11729675 None:intergenic 30.0%
CATCTTCTTCAAGCTAAAAA+AGG - chr3_3:11729654-11729673 Msa0442260:CDS 30.0%
TTTAGTCCCACTTCAAATAT+TGG + chr3_3:11729977-11729996 None:intergenic 30.0%
! TATTTGAAGTGGGACTAAAA+TGG - chr3_3:11729978-11729997 Msa0442260:intron 30.0%
TGATCTCACATAAAGCTTAA+TGG + chr3_3:11730009-11730028 None:intergenic 30.0%
TGACTTCAAATCTTTGGATT+TGG - chr3_3:11730047-11730066 Msa0442260:intron 30.0%
CTATACTTTAGACCTCAATT+TGG + chr3_3:11730106-11730125 None:intergenic 30.0%
! CAAATTGAGGTCTAAAGTAT+AGG - chr3_3:11730104-11730123 Msa0442260:intron 30.0%
ATGATAACTTAGGGCTTATT+TGG - chr3_3:11730133-11730152 Msa0442260:intron 30.0%
TGATAACTTAGGGCTTATTT+GGG - chr3_3:11730134-11730153 Msa0442260:intron 30.0%
ACACACATACATTAGTATTG+GGG - chr3_3:11730403-11730422 Msa0442260:intron 30.0%
! ACAGCAATTTTTCCAGAATT+TGG + chr3_3:11730453-11730472 None:intergenic 30.0%
GATAAAATATACCGTCTTTG+TGG - chr3_3:11730474-11730493 Msa0442260:intron 30.0%
GATCTAATCATGTTCAAAGT+TGG + chr3_3:11730571-11730590 None:intergenic 30.0%
TGATTAGATCTTCGTATTCT+TGG - chr3_3:11730581-11730600 Msa0442260:intron 30.0%
AACAGCTATTATCTAGAGAA+CGG + chr3_3:11730609-11730628 None:intergenic 30.0%
! AGAATTAGGCTCTTTCTTTT+TGG - chr3_3:11730714-11730733 Msa0442260:intron 30.0%
ATTCATTACTGAACTCATGT+AGG - chr3_3:11730809-11730828 Msa0442260:intron 30.0%
ACTACAATGCTATAACATAG+TGG - chr3_3:11730897-11730916 Msa0442260:intron 30.0%
ATAATCACAAGCAAATGCAA+AGG - chr3_3:11731240-11731259 Msa0442260:intron 30.0%
!! GATTTGATTTGCAGTATCAT+CGG + chr3_3:11731478-11731497 None:intergenic 30.0%
ACAACACTCAAATACAACAT+AGG + chr3_3:11731727-11731746 None:intergenic 30.0%
!! TTAGAGATTGCAAGTTTTGT+TGG - chr3_3:11731747-11731766 Msa0442260:five_prime_UTR 30.0%
!!! AGTTTTGTTGAGAAGAAGCT+TGG + chr3_3:11729212-11729231 None:intergenic 35.0%
TTCTTCACTTCTTCTCATCA+TGG - chr3_3:11729432-11729451 Msa0442260:CDS 35.0%
GAGTTACTAGGAAGAAAAGT+TGG + chr3_3:11729481-11729500 None:intergenic 35.0%
AGAAGGAAAAGAGAGTTACT+AGG + chr3_3:11729493-11729512 None:intergenic 35.0%
!!! AATTTTGAAGCGCCAAATTG+AGG - chr3_3:11730091-11730110 Msa0442260:intron 35.0%
GCTATCCTATATAGAGCTTA+TGG + chr3_3:11730255-11730274 None:intergenic 35.0%
ACTAATGTATGTGTGTAACC+TGG + chr3_3:11730399-11730418 None:intergenic 35.0%
! ACATACATTAGTATTGGGGA+TGG - chr3_3:11730407-11730426 Msa0442260:intron 35.0%
!! GATGGACTAATAGAGAAGTA+TGG - chr3_3:11730425-11730444 Msa0442260:intron 35.0%
ATAGCTGTTTGAGAAAGATC+TGG - chr3_3:11730620-11730639 Msa0442260:intron 35.0%
TTGCATTGAATCAATGCAAC+TGG + chr3_3:11730743-11730762 None:intergenic 35.0%
TACACCATGTTGTCAAATAC+AGG - chr3_3:11730961-11730980 Msa0442260:intron 35.0%
ACAACCTGTATTTGACAACA+TGG + chr3_3:11730968-11730987 None:intergenic 35.0%
TTGTCAAATACAGGTTGTAG+CGG - chr3_3:11730970-11730989 Msa0442260:intron 35.0%
!!! ACGTTATTTTTCACAATCCG+CGG - chr3_3:11731030-11731049 Msa0442260:intron 35.0%
! ACTGATCTATAGCAGAGTTT+TGG - chr3_3:11731061-11731080 Msa0442260:intron 35.0%
AATGTAGCTAAACATGGTTG+AGG + chr3_3:11731358-11731377 None:intergenic 35.0%
TTTGATGCTGAGACACTTAC+TGG - chr3_3:11729627-11729646 Msa0442260:CDS 40.0%
AAGTTTATCGAAACACAGCC+AGG - chr3_3:11730378-11730397 Msa0442260:intron 40.0%
! AGAAGTATGGCTCCAAATTC+TGG - chr3_3:11730438-11730457 Msa0442260:intron 40.0%
ATACATGGAAGCCACAAAGA+CGG + chr3_3:11730488-11730507 None:intergenic 40.0%
TGTCAAATACAGGTTGTAGC+GGG - chr3_3:11730971-11730990 Msa0442260:intron 40.0%
!!! ATAGCAGAGTTTTGGTTGTG+TGG - chr3_3:11731069-11731088 Msa0442260:intron 40.0%
! ATCAGCAAACGCTTCTCTTT+TGG + chr3_3:11731159-11731178 None:intergenic 40.0%
AATGCTCCACAACATTCCTT+GGG + chr3_3:11731279-11731298 None:intergenic 40.0%
AAATGCTCCACAACATTCCT+TGG + chr3_3:11731280-11731299 None:intergenic 40.0%
!! TTGATTTGCAGTATCATCGG+CGG + chr3_3:11731475-11731494 None:intergenic 40.0%
CACGTTCGTTATTGTTGATG+CGG - chr3_3:11731642-11731661 Msa0442260:five_prime_UTR 40.0%
ACGTTCGTTATTGTTGATGC+GGG - chr3_3:11731643-11731662 Msa0442260:five_prime_UTR 40.0%
ACTATGACACTGTTGCGTAG+CGG - chr3_3:11730995-11731014 Msa0442260:intron 45.0%
! TTTTCACAATCCGCGGTTGA+CGG - chr3_3:11731037-11731056 Msa0442260:intron 45.0%
TTGTTTCACATTGCACCTGC+AGG - chr3_3:11731183-11731202 Msa0442260:intron 45.0%
AAATTCACAGAACAGCCTGC+AGG + chr3_3:11731201-11731220 None:intergenic 45.0%
CTTTATGGAAGATACCCTCC+TGG - chr3_3:11731381-11731400 Msa0442260:intron 45.0%
ACATACTCCATCTCTAGTGC+TGG - chr3_3:11731500-11731519 Msa0442260:CDS 45.0%
! GTGTTGTTACATAGCCGCTG+TGG - chr3_3:11730870-11730889 Msa0442260:intron 50.0%
!! CATTCCTTGGGAGGGTACTT+AGG + chr3_3:11731267-11731286 None:intergenic 50.0%
TACCCTCCCAAGGAATGTTG+TGG - chr3_3:11731270-11731289 Msa0442260:intron 50.0%
CTCCACAACATTCCTTGGGA+GGG + chr3_3:11731275-11731294 None:intergenic 50.0%
GCATTGTAGTGCTGCCACAG+CGG + chr3_3:11730887-11730906 None:intergenic 55.0%
TAGATCAGTGCCGTCAACCG+CGG + chr3_3:11731050-11731069 None:intergenic 55.0%
AGGACCTAAGTACCCTCCCA+AGG - chr3_3:11731260-11731279 Msa0442260:intron 55.0%
GCTCCACAACATTCCTTGGG+AGG + chr3_3:11731276-11731295 None:intergenic 55.0%
! TTTGCGAGCGAGTGTCGTGA+AGG - chr3_3:11731408-11731427 Msa0442260:intron 55.0%
! TTGCGAGCGAGTGTCGTGAA+GGG - chr3_3:11731409-11731428 Msa0442260:intron 55.0%
! GAGTGTCGTGAAGGGAAGGA+AGG - chr3_3:11731417-11731436 Msa0442260:intron 55.0%
GTATCATCGGCGGACACTGA+TGG + chr3_3:11731465-11731484 None:intergenic 55.0%
GTGAGCACCAGCACTAGAGA+TGG + chr3_3:11731510-11731529 None:intergenic 55.0%
CACGACACTCGCTCGCAAAG+AGG + chr3_3:11731407-11731426 None:intergenic 60.0%
! GAGCGAGTGTCGTGAAGGGA+AGG - chr3_3:11731413-11731432 Msa0442260:intron 60.0%
TCATCGGCGGACACTGATGG+TGG + chr3_3:11731462-11731481 None:intergenic 60.0%
CGCTCGCAAAGAGGCCAGGA+GGG + chr3_3:11731398-11731417 None:intergenic 65.0%
TCGCTCGCAAAGAGGCCAGG+AGG + chr3_3:11731399-11731418 None:intergenic 65.0%
CACTCGCTCGCAAAGAGGCC+AGG + chr3_3:11731402-11731421 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 11729168 11731817 11729168 ID=Msa0442260;Name=Msa0442260
chr3_3 mRNA 11729168 11731817 11729168 ID=Msa0442260-mRNA-1;Parent=Msa0442260;Name=Msa0442260-mRNA-1;_AED=0.03;_eAED=0.03;_QI=226|1|1|1|1|1|3|257|170
chr3_3 exon 11729168 11729780 11729168 ID=Msa0442260-mRNA-1:exon:2491;Parent=Msa0442260-mRNA-1
chr3_3 exon 11731310 11731366 11731310 ID=Msa0442260-mRNA-1:exon:2490;Parent=Msa0442260-mRNA-1
chr3_3 exon 11731492 11731817 11731492 ID=Msa0442260-mRNA-1:exon:2489;Parent=Msa0442260-mRNA-1
chr3_3 five_prime_UTR 11731592 11731817 11731592 ID=Msa0442260-mRNA-1:five_prime_utr;Parent=Msa0442260-mRNA-1
chr3_3 CDS 11731492 11731591 11731492 ID=Msa0442260-mRNA-1:cds;Parent=Msa0442260-mRNA-1
chr3_3 CDS 11731310 11731366 11731310 ID=Msa0442260-mRNA-1:cds;Parent=Msa0442260-mRNA-1
chr3_3 CDS 11729425 11729780 11729425 ID=Msa0442260-mRNA-1:cds;Parent=Msa0442260-mRNA-1
chr3_3 three_prime_UTR 11729168 11729424 11729168 ID=Msa0442260-mRNA-1:three_prime_utr;Parent=Msa0442260-mRNA-1
Gene Sequence

>Msa0442260

ATGGCTTGTTCTATGAATCAACAAAGCTTCTTGCTTTGTTCTTCACTTCTTCTCATCATGGCTGTTTCTGTTTCTTCTTTTCATCCAACTTTTCTTCCTAATACTATTTTTGATGCTGAGACACTTACTGGTCGCCATCTTCTTCAAGCTAAAAAAGGCTGTTCTGTGAATTTTGAGTTTCTAAACTATACAATAATCACAAGCAAATGCAAAGGACCTAAGTACCCTCCCAAGGAATGTTGTGGAGCATTTAAAGAGTTTGCTTGCCCTTATACTGATGTGATAAATGATTTAACAAACGATTGTGCCTCAACCATGTTTAGCTACATTAATCTTTATGGAAGATACCCTCCTGGCCTCTTTGCGAGCGAGTGTCGTGAAGGGAAGGAAGGTCTAGCATGTGATGCATTGCCACCATCAGTGTCCGCCGATGATACTGCAAATCAAATCGTACATACTCCATCTCTAGTGCTGGTGCTCACAGCCTGCATCTTCTTAATTTTAATATTCTGA

Protein sequence

>Msa0442260

MACSMNQQSFLLCSSLLLIMAVSVSSFHPTFLPNTIFDAETLTGRHLLQAKKGCSVNFEFLNYTIITSKCKGPKYPPKECCGAFKEFACPYTDVINDLTNDCASTMFSYINLYGRYPPGLFASECREGKEGLACDALPPSVSADDTANQIVHTPSLVLVLTACIFLILIF*