AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0650970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650970 MtrunA17_Chr4g0043301 99.200 500 4 0 1 500 1 500 0.0 1022
Msa0650970 MtrunA17_Chr7g0263711 93.600 500 32 0 1 500 1 500 0.0 969
Msa0650970 MtrunA17_Chr4g0043311 89.336 497 53 0 4 500 1 497 0.0 926
Msa0650970 MtrunA17_Chr6g0448901 78.819 491 104 0 10 500 20 510 0.0 818
Msa0650970 MtrunA17_Chr7g0265591 42.485 499 275 7 12 500 30 526 1.31e-128 384
Msa0650970 MtrunA17_Chr1g0197911 41.748 515 282 9 1 500 14 525 2.63e-127 380
Msa0650970 MtrunA17_Chr1g0179541 41.517 501 263 7 12 500 30 512 6.11e-122 367
Msa0650970 MtrunA17_Chr5g0400361 38.780 410 237 8 6 412 73 471 4.73e-89 283
Msa0650970 MtrunA17_Chr6g0463101 35.787 489 265 12 9 486 104 554 4.68e-84 270
Msa0650970 MtrunA17_Chr1g0202291 35.174 489 268 12 9 486 102 552 1.54e-83 269
Msa0650970 MtrunA17_Chr1g0188101 31.600 500 285 11 11 485 90 557 5.75e-71 236
Msa0650970 MtrunA17_Chr1g0206791 32.383 491 294 11 11 488 17 482 2.11e-69 230
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650970 AT5G56350.1 89.271 494 53 0 7 500 5 498 0.0 908
Msa0650970 AT4G26390.1 83.603 494 81 0 7 500 4 497 0.0 867
Msa0650970 AT5G08570.1 79.022 491 103 0 10 500 20 510 0.0 821
Msa0650970 AT5G08570.2 79.022 491 103 0 10 500 20 510 0.0 821
Msa0650970 AT5G08570.3 79.022 491 103 0 10 500 20 510 0.0 821
Msa0650970 AT5G63680.3 78.702 493 105 0 8 500 18 510 0.0 818
Msa0650970 AT5G63680.1 78.702 493 105 0 8 500 18 510 0.0 818
Msa0650970 AT5G63680.2 78.702 493 105 0 8 500 18 510 0.0 818
Msa0650970 AT3G04050.1 73.790 496 126 3 9 500 15 510 0.0 758
Msa0650970 AT3G25960.1 72.470 494 123 4 9 500 15 497 0.0 728
Msa0650970 AT3G55650.1 71.169 496 139 3 9 500 15 510 0.0 726
Msa0650970 AT3G55810.1 67.944 496 137 4 9 500 15 492 0.0 673
Msa0650970 AT2G36580.1 43.487 499 270 7 12 500 30 526 3.03e-130 389
Msa0650970 AT3G52990.1 43.287 499 271 7 12 500 30 526 2.82e-129 386
Msa0650970 AT3G52990.2 42.526 475 261 7 36 500 1 473 3.76e-120 361
Msa0650970 AT5G52920.1 36.179 492 272 13 11 495 111 567 1.42e-84 272
Msa0650970 AT1G32440.1 39.320 412 229 10 7 412 96 492 4.21e-81 263
Msa0650970 AT3G22960.1 31.717 495 289 12 11 485 118 583 3.25e-70 235
Msa0650970 AT3G49160.3 31.618 272 159 9 73 322 325 591 1.55e-21 98.6
Msa0650970 AT3G49160.1 31.618 272 159 9 73 322 413 679 1.75e-21 98.6

Find 136 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAACTCTGATGCATTTA+TGG 0.128456 4_3:-58265009 Msa0650970:CDS
AAGCGGTTTCAGTTGTTTAT+TGG 0.237023 4_3:-58267072 Msa0650970:five_prime_UTR
CAAAGTTTGCAACCCCTTCT+TGG 0.251729 4_3:+58265042 None:intergenic
CGTGCAAACGATCTTAGTTT+TGG 0.261618 4_3:+58266958 None:intergenic
TCTCAACAATAACAATAATA+AGG 0.268598 4_3:+58264074 None:intergenic
GAGTGGTGTGGTGGTTCTTC+TGG 0.285238 4_3:+58267026 None:intergenic
AGGAGGGACTACTGCAAAAT+TGG 0.314986 4_3:-58264562 Msa0650970:CDS
GTACAAGTTCGAAAACTTCT+AGG 0.320879 4_3:-58265385 Msa0650970:CDS
TGTGAGAACTCTGCTGTTCT+AGG 0.329692 4_3:-58265553 Msa0650970:CDS
GCAGAAGCAGCACTTAATAT+TGG 0.330688 4_3:+58264411 None:intergenic
TGATGTTGCGAATGCAGTTC+TGG 0.344149 4_3:-58264823 Msa0650970:CDS
GAAAGGGACTCTGCGTTAAC+GGG 0.361244 4_3:-58264326 Msa0650970:CDS
CTTATCGTGTGACAAAGAAT+TGG 0.361638 4_3:-58265593 Msa0650970:CDS
TTGTATTCTCATCACCCTTT+AGG 0.370256 4_3:+58265701 None:intergenic
CGATAAGACTGTAAATGATA+TGG 0.371207 4_3:+58265609 None:intergenic
AGCTTCAGCTCTGGTTGGCC+GGG 0.390347 4_3:+58264848 None:intergenic
TAGCTTCAGCTCTGGTTGGC+CGG 0.391145 4_3:+58264847 None:intergenic
CAAGGAAGATATCATGGCTT+GGG 0.397205 4_3:-58265467 Msa0650970:CDS
CCTTTCCCTTGGCACATTGA+AGG 0.403085 4_3:+58264343 None:intergenic
GCTGAGAGCACCATTGACTA+TGG 0.403578 4_3:-58264708 Msa0650970:CDS
GGAAAGGGACTCTGCGTTAA+CGG 0.404260 4_3:-58264327 Msa0650970:CDS
CGCCGTCATGCTCGATACTA+AGG 0.406356 4_3:-58266780 Msa0650970:intron
TCAACTGACTATAGCCTAAA+GGG 0.420476 4_3:-58265715 Msa0650970:CDS
CATGACGGCGCAGAGAATTC+CGG 0.420747 4_3:+58266793 None:intergenic
CATCCAACGTAGACTGTAGA+TGG 0.432323 4_3:+58264163 None:intergenic
TTGTTTCAGTTGGATAGGTT+TGG 0.435015 4_3:+58265756 None:intergenic
TGCTAGAGCAGCTCTCATAT+TGG 0.437951 4_3:-58264595 Msa0650970:CDS
AGATGTATTCAAAAGGATAA+TGG 0.438677 4_3:-58264685 Msa0650970:CDS
GGAACGACAACAGAAAGAAT+TGG 0.441249 4_3:+58264516 None:intergenic
GCACGCTGGGACCCGCATCT+AGG 0.445382 4_3:-58266940 Msa0650970:CDS
TTATCGTGTGACAAAGAATT+GGG 0.446712 4_3:-58265592 Msa0650970:CDS
ACAGAAAGAATTGGCGTGCC+TGG 0.449963 4_3:+58264525 None:intergenic
TATCATGATTGAGAAGTGTA+TGG 0.450523 4_3:-58263927 Msa0650970:three_prime_UTR
GCGCTTTCATTTGTTCGAAA+AGG 0.450686 4_3:-58265418 Msa0650970:CDS
CAAAATTGGTGGCTAAATAC+AGG 0.450918 4_3:-58264548 Msa0650970:CDS
TTTCATTACCTTGTTTCAGT+TGG 0.451778 4_3:+58265746 None:intergenic
TTACCTTGTTTCAGTTGGAT+AGG 0.452533 4_3:+58265751 None:intergenic
TGTTCGAAAAGGTTCTGATC+TGG 0.452582 4_3:-58265407 Msa0650970:CDS
TCCAATGGGCTCATGGGTAC+TGG 0.457116 4_3:+58264654 None:intergenic
TAACGACTGATACCTTTGAT+TGG 0.457143 4_3:-58264488 Msa0650970:CDS
AGAAATCCAGTTCGAATCTC+AGG 0.458723 4_3:+58265787 None:intergenic
ACTAAGATCGTTTGCACGCT+GGG 0.459837 4_3:-58266953 Msa0650970:CDS
GAGAGCTGCTCTAGCAGAGT+TGG 0.462449 4_3:+58264602 None:intergenic
CTTTCCCTTGGCACATTGAA+GGG 0.463400 4_3:+58264344 None:intergenic
ATTGTACGAACAGCAAGTTC+TGG 0.463793 4_3:+58264750 None:intergenic
GAATACATCTCCATAGTCAA+TGG 0.466000 4_3:+58264698 None:intergenic
TAACAATAATAAGGTAATAG+TGG 0.466373 4_3:+58264083 None:intergenic
AACGCAGAGTCCCTTTCCCT+TGG 0.468309 4_3:+58264332 None:intergenic
CGCAAAAGCTTCTCGACCAT+GGG 0.471680 4_3:+58266911 None:intergenic
TAGACTCTCCAATGGGCTCA+TGG 0.478114 4_3:+58264647 None:intergenic
TTGGCTCATGATGTGAAGCC+TGG 0.480964 4_3:-58265658 Msa0650970:CDS
CAATTGGAATTTCCATTCCA+AGG 0.480988 4_3:+58264973 None:intergenic
GCTAGATTCAACTTCTCTCA+TGG 0.485672 4_3:-58266872 Msa0650970:CDS
GTGTGACAAAGAATTGGGTT+TGG 0.488491 4_3:-58265587 Msa0650970:CDS
GTGCAATATCCAAGGAAAAC+CGG 0.489545 4_3:-58264913 Msa0650970:CDS
AGAAAGAATGTTAATCTGCC+TGG 0.492316 4_3:-58265526 Msa0650970:CDS
AATATCAAGCTATGTCTAGC+AGG 0.493341 4_3:+58264447 None:intergenic
TAGGGGCCTGAGATTCGAAC+TGG 0.499972 4_3:-58265793 Msa0650970:CDS
CTTCAATGTGCCAAGGGAAA+GGG 0.500302 4_3:-58264342 Msa0650970:CDS
TTCAACTGACTATAGCCTAA+AGG 0.500418 4_3:-58265716 Msa0650970:CDS
CGTTAACGGGGATTCTGTTG+TGG 0.503390 4_3:-58264313 Msa0650970:CDS
GCTTCAGCTCTGGTTGGCCG+GGG 0.507929 4_3:+58264849 None:intergenic
CGTTACTGCGACTCAGATGT+TGG 0.507988 4_3:-58264889 Msa0650970:CDS
ATCTGAGTCGCAGTAACGAC+CGG 0.509493 4_3:+58264894 None:intergenic
GAAAGACAAGGAAGATATCA+TGG 0.513280 4_3:-58265473 Msa0650970:CDS
TGGTTCTCATGAGTATCATC+AGG 0.514740 4_3:-58266852 Msa0650970:CDS
CCTTCAATGTGCCAAGGGAA+AGG 0.523286 4_3:-58264343 Msa0650970:CDS
ACAGACTGTGTCATGCTTAG+TGG 0.525623 4_3:-58264795 Msa0650970:CDS
ACAAGGAAGATATCATGGCT+TGG 0.526189 4_3:-58265468 Msa0650970:CDS
AAGGTATCAGTCGTTAGCTC+AGG 0.530961 4_3:+58264495 None:intergenic
CGCATCTAGGTCTATTCCCA+TGG 0.531643 4_3:-58266927 Msa0650970:CDS
TGATAATCTTAGAGCTGCCA+TGG 0.531806 4_3:-58266822 Msa0650970:CDS
AGTGTCATACTCTGCGCAGA+TGG 0.532331 4_3:-58265634 Msa0650970:CDS
ACTATGGAGATGTATTCAAA+AGG 0.538144 4_3:-58264692 Msa0650970:CDS
GGAAGATCCACTACGACTCC+AGG 0.538876 4_3:+58265508 None:intergenic
CTTGTCTTTCTCAGTCAAAG+TGG 0.540820 4_3:+58265486 None:intergenic
AACTAAGATCGTTTGCACGC+TGG 0.542989 4_3:-58266954 Msa0650970:CDS
TCGCAAAAGCTTCTCGACCA+TGG 0.546576 4_3:+58266910 None:intergenic
CTTAGTGGTGAAACTGCTGC+TGG 0.547208 4_3:-58264780 Msa0650970:CDS
AACATCAGTAGCTTCAGCTC+TGG 0.547477 4_3:+58264839 None:intergenic
GGCAGGTTGAAAACCAAGAA+GGG 0.548016 4_3:-58265055 Msa0650970:intron
GAACATTCTTCTCATGTCAA+AGG 0.556045 4_3:-58265350 Msa0650970:intron
AGACTCTCCAATGGGCTCAT+GGG 0.558290 4_3:+58264648 None:intergenic
AGGGACTACTGCAAAATTGG+TGG 0.560616 4_3:-58264559 Msa0650970:CDS
CTCATATTGGTTCTAACTAG+AGG 0.560702 4_3:-58264582 Msa0650970:CDS
TTAATATTGGAATCAATCCT+CGG 0.562918 4_3:+58264424 None:intergenic
AGAGCTGCCATGGAGAATAC+CGG 0.568658 4_3:-58266812 Msa0650970:CDS
ACCAGTACCCATGAGCCCAT+TGG 0.569139 4_3:-58264655 Msa0650970:CDS
TTTGAGAATATCTATATAAG+CGG 0.569632 4_3:-58267089 Msa0650970:five_prime_UTR
TTGTCTTTCTCAGTCAAAGT+GGG 0.570627 4_3:+58265487 None:intergenic
TTTAATGGGAGATCATTTCA+CGG 0.575483 4_3:+58264248 None:intergenic
GGCACGTGGTGACCTTGGAA+TGG 0.579482 4_3:-58264985 Msa0650970:CDS
AAGAAGCTAGACTCTCCAAT+GGG 0.579536 4_3:+58264640 None:intergenic
TTGGTGGCTAAATACAGGCC+AGG 0.581932 4_3:-58264543 Msa0650970:CDS
TGGCAGGTTGAAAACCAAGA+AGG 0.582341 4_3:-58265056 Msa0650970:intron
ATATCAAGCTATGTCTAGCA+GGG 0.582962 4_3:+58264448 None:intergenic
TCAGTAGCTTCAGCTCTGGT+TGG 0.584103 4_3:+58264843 None:intergenic
TGTTGTGGTTCTGCATCGCG+TGG 0.586595 4_3:-58264298 Msa0650970:CDS
GATTCGAACTGGATTTCTCA+AGG 0.588567 4_3:-58265782 Msa0650970:CDS
ATTTGCCCTTCAATGTGCCA+AGG 0.591498 4_3:-58264349 Msa0650970:CDS
TAAATGCATCAGAGTTTGCA+AGG 0.592927 4_3:+58265012 None:intergenic
ATGGTGGCACGTGGTGACCT+TGG 0.597261 4_3:-58264990 Msa0650970:CDS
ATTTATAAGTGCAATATCCA+AGG 0.598187 4_3:-58264921 Msa0650970:CDS
AGAACTTGCTGTTCGTACAA+TGG 0.602563 4_3:-58264748 Msa0650970:CDS
AGACATAGCTTGATATTCCG+AGG 0.602907 4_3:-58264441 Msa0650970:CDS
GCGCAGAGTATGACACTCCC+AGG 0.606193 4_3:+58265640 None:intergenic
ATATTGGTTCTAACTAGAGG+AGG 0.607238 4_3:-58264579 Msa0650970:CDS
CACTTTGACTGAGAAAGACA+AGG 0.607462 4_3:-58265485 Msa0650970:CDS
TGAGAAGAATGTTCTTAGCA+TGG 0.609210 4_3:+58265359 None:intergenic
TTTGCCCTTCAATGTGCCAA+GGG 0.609763 4_3:-58264348 Msa0650970:CDS
GTTGCGAATGCAGTTCTGGA+TGG 0.610844 4_3:-58264819 Msa0650970:CDS
GAGAATTCCGGTATTCTCCA+TGG 0.611587 4_3:+58266805 None:intergenic
ATCACTGCATGACCAATCAA+AGG 0.619997 4_3:+58264476 None:intergenic
GAAGAAGCTAGACTCTCCAA+TGG 0.622268 4_3:+58264639 None:intergenic
TGGCTCATGATGTGAAGCCT+GGG 0.622471 4_3:-58265657 Msa0650970:CDS
TAATCTGCCTGGAGTCGTAG+TGG 0.623976 4_3:-58265515 Msa0650970:CDS
ATGGGAATAGACCTAGATGC+GGG 0.628393 4_3:+58266929 None:intergenic
TATTGGTTCTAACTAGAGGA+GGG 0.629589 4_3:-58264578 Msa0650970:CDS
AAACCTATCCAACTGAAACA+AGG 0.639584 4_3:-58265754 Msa0650970:CDS
AAGGAAGATATCATGGCTTG+GGG 0.639801 4_3:-58265466 Msa0650970:CDS
TCATCTCATCGATCTAAACA+TGG 0.643002 4_3:-58267005 Msa0650970:exon
CGATGAGATGAGTGGTGTGG+TGG 0.644965 4_3:+58267017 None:intergenic
AGTCAATGATCAAATCTCCC+CGG 0.646746 4_3:-58264866 Msa0650970:CDS
AAAGGGACTCTGCGTTAACG+GGG 0.647503 4_3:-58264325 Msa0650970:CDS
GTTGTGGTTCTGCATCGCGT+GGG 0.653541 4_3:-58264297 Msa0650970:CDS
GCAGGTTGAAAACCAAGAAG+GGG 0.675723 4_3:-58265054 Msa0650970:intron
TCAAACAAGCAAGTCTCACG+CGG 0.683267 4_3:+58267107 None:intergenic
ATACCATCTACAGTCTACGT+TGG 0.683554 4_3:-58264166 Msa0650970:three_prime_UTR
TACCTTAGTATCGAGCATGA+CGG 0.683613 4_3:+58266778 None:intergenic
CATGGGAATAGACCTAGATG+CGG 0.689751 4_3:+58266928 None:intergenic
GATCGATGAGATGAGTGGTG+TGG 0.702627 4_3:+58267014 None:intergenic
AAACTCTGATGCATTTATGG+TGG 0.705208 4_3:-58265006 Msa0650970:CDS
TATCAAGCTATGTCTAGCAG+GGG 0.708675 4_3:+58264449 None:intergenic
GTTTAGATCGATGAGATGAG+TGG 0.731788 4_3:+58267009 None:intergenic
GATGCATTTATGGTGGCACG+TGG 0.742236 4_3:-58264999 Msa0650970:CDS
GAACAGCAGAGTTCTCACAG+CGG 0.862341 4_3:+58265557 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAAAAAATAAAAAA+AGG + chr4_3:58264319-58264338 None:intergenic 0.0%
!!! ATAAAAAATCACATTTTAAT+GGG + chr4_3:58266835-58266854 None:intergenic 10.0%
!!! CTGAAAATAATTTTTGTATT+TGG - chr4_3:58264778-58264797 Msa0650970:CDS 15.0%
!! TACTTGATTCTTAATATTTA+TGG - chr4_3:58265099-58265118 Msa0650970:intron 15.0%
!!! GATAAAAAATCACATTTTAA+TGG + chr4_3:58266836-58266855 None:intergenic 15.0%
!!! TGTTTTTGATTATTATTTTG+CGG - chr4_3:58267089-58267108 Msa0650970:five_prime_UTR 15.0%
!!! CTACTTTTGTTGAATAATTT+TGG - chr4_3:58263935-58263954 Msa0650970:three_prime_UTR 20.0%
!! TTTGAGAATATCTATATAAG+CGG - chr4_3:58263977-58263996 Msa0650970:three_prime_UTR 20.0%
!! AATTCTTATCCATACATATA+AGG + chr4_3:58264541-58264560 None:intergenic 20.0%
!! TAAAAGAACTATAGTCTATT+TGG + chr4_3:58265031-58265050 None:intergenic 20.0%
!! ATTAAGAATCAAGTAAAAAC+CGG + chr4_3:58265094-58265113 None:intergenic 20.0%
!! TAACAATAATAAGGTAATAG+TGG + chr4_3:58266986-58267005 None:intergenic 20.0%
!! TCTCAACAATAACAATAATA+AGG + chr4_3:58266995-58267014 None:intergenic 20.0%
!!! TTTTTTGTACTCAAAAATGT+AGG - chr4_3:58267058-58267077 Msa0650970:five_prime_UTR 20.0%
!!! TGATTTTGACCTTATATGTA+TGG - chr4_3:58264529-58264548 Msa0650970:CDS 25.0%
! TGTTAGATTAGGTTATGTTT+GGG - chr4_3:58264615-58264634 Msa0650970:CDS 25.0%
!! TTTTATAAGATGTCTTAGAG+AGG - chr4_3:58264742-58264761 Msa0650970:CDS 25.0%
!!! TATGGTTTAGAACCTTATTT+TGG - chr4_3:58265117-58265136 Msa0650970:intron 25.0%
! TTACAATACTCACCAAAATA+AGG + chr4_3:58265132-58265151 None:intergenic 25.0%
!!! TATTAAGAGAGTTTTAGTTG+CGG + chr4_3:58265177-58265196 None:intergenic 25.0%
!! GCAATCATGTCAATTTTATT+GGG + chr4_3:58265630-58265649 None:intergenic 25.0%
! AAGAGATAGAAAAAATCAGT+AGG + chr4_3:58265928-58265947 None:intergenic 25.0%
! ATTTATAAGTGCAATATCCA+AGG - chr4_3:58266145-58266164 Msa0650970:intron 25.0%
! AGATGTATTCAAAAGGATAA+TGG - chr4_3:58266381-58266400 Msa0650970:intron 25.0%
! TTAATATTGGAATCAATCCT+CGG + chr4_3:58266645-58266664 None:intergenic 25.0%
!!! GTGATTTTTTATCTGCTTTT+TGG - chr4_3:58266843-58266862 Msa0650970:CDS 25.0%
!! ACATCAATTGATGCTTTTAT+TGG - chr4_3:58266930-58266949 Msa0650970:CDS 25.0%
!! CATCAATTGATGCTTTTATT+GGG - chr4_3:58266931-58266950 Msa0650970:CDS 25.0%
!! ATCAATTGATGCTTTTATTG+GGG - chr4_3:58266932-58266951 Msa0650970:CDS 25.0%
! ATGCAAATACGTGTAAAAAA+GGG + chr4_3:58266962-58266981 None:intergenic 25.0%
! TATGCAAATACGTGTAAAAA+AGG + chr4_3:58266963-58266982 None:intergenic 25.0%
CGGTAAAAGTCACTATTAAT+CGG + chr4_3:58264578-58264597 None:intergenic 30.0%
ACAGTTCAAACATGTATTGA+CGG + chr4_3:58264598-58264617 None:intergenic 30.0%
CATGTTTGAACTGTTAGATT+AGG - chr4_3:58264604-58264623 Msa0650970:CDS 30.0%
CTGTTAGATTAGGTTATGTT+TGG - chr4_3:58264614-58264633 Msa0650970:CDS 30.0%
TGTTTCATGCAAGTTATTAG+CGG - chr4_3:58264718-58264737 Msa0650970:CDS 30.0%
!! ATAAAAGCATCTTCAATGTC+CGG - chr4_3:58265072-58265091 Msa0650970:intron 30.0%
! TTTCATTACCTTGTTTCAGT+TGG + chr4_3:58265323-58265342 None:intergenic 30.0%
CGATAAGACTGTAAATGATA+TGG + chr4_3:58265460-58265479 None:intergenic 30.0%
TTATCGTGTGACAAAGAATT+GGG - chr4_3:58265474-58265493 Msa0650970:CDS 30.0%
! CGCAATCATGTCAATTTTAT+TGG + chr4_3:58265631-58265650 None:intergenic 30.0%
! GCGAGAAATATTTTCTCAAT+TGG + chr4_3:58266112-58266131 None:intergenic 30.0%
ACTATGGAGATGTATTCAAA+AGG - chr4_3:58266374-58266393 Msa0650970:intron 30.0%
!! CAAGATTTTGATGATTGATG+CGG + chr4_3:58266797-58266816 None:intergenic 30.0%
TTTAATGGGAGATCATTTCA+CGG + chr4_3:58266821-58266840 None:intergenic 30.0%
!! TTTGGCGAAGTATTTTATGA+TGG - chr4_3:58266861-58266880 Msa0650970:CDS 30.0%
! TATCATGATTGAGAAGTGTA+TGG - chr4_3:58267139-58267158 Msa0650970:five_prime_UTR 30.0%
AAGCGGTTTCAGTTGTTTAT+TGG - chr4_3:58263994-58264013 Msa0650970:three_prime_UTR 35.0%
TCATCTCATCGATCTAAACA+TGG - chr4_3:58264061-58264080 Msa0650970:three_prime_UTR 35.0%
!! GTGCAAACGATCTTAGTTTT+GGG + chr4_3:58264110-58264129 None:intergenic 35.0%
AAGATGTCTTAGAGAGGTTA+TGG - chr4_3:58264748-58264767 Msa0650970:CDS 35.0%
! TTGGTGAGTATTGTAATTCG+AGG - chr4_3:58265136-58265155 Msa0650970:intron 35.0%
! TGGTGAGTATTGTAATTCGA+GGG - chr4_3:58265137-58265156 Msa0650970:intron 35.0%
ATGAAGCAACTTGAAAATGC+TGG + chr4_3:58265208-58265227 None:intergenic 35.0%
TTACATTGCTCATTGCTGTA+GGG - chr4_3:58265255-58265274 Msa0650970:intron 35.0%
TTGTTTCAGTTGGATAGGTT+TGG + chr4_3:58265313-58265332 None:intergenic 35.0%
AAACCTATCCAACTGAAACA+AGG - chr4_3:58265312-58265331 Msa0650970:intron 35.0%
! TTACCTTGTTTCAGTTGGAT+AGG + chr4_3:58265318-58265337 None:intergenic 35.0%
TTCAACTGACTATAGCCTAA+AGG - chr4_3:58265350-58265369 Msa0650970:intron 35.0%
TCAACTGACTATAGCCTAAA+GGG - chr4_3:58265351-58265370 Msa0650970:CDS 35.0%
TTGTATTCTCATCACCCTTT+AGG + chr4_3:58265368-58265387 None:intergenic 35.0%
CTTATCGTGTGACAAAGAAT+TGG - chr4_3:58265473-58265492 Msa0650970:CDS 35.0%
AGAAAGAATGTTAATCTGCC+TGG - chr4_3:58265540-58265559 Msa0650970:CDS 35.0%
TTGTCTTTCTCAGTCAAAGT+GGG + chr4_3:58265582-58265601 None:intergenic 35.0%
GAAAGACAAGGAAGATATCA+TGG - chr4_3:58265593-58265612 Msa0650970:CDS 35.0%
GTACAAGTTCGAAAACTTCT+AGG - chr4_3:58265681-58265700 Msa0650970:CDS 35.0%
TGAGAAGAATGTTCTTAGCA+TGG + chr4_3:58265710-58265729 None:intergenic 35.0%
GAACATTCTTCTCATGTCAA+AGG - chr4_3:58265716-58265735 Msa0650970:CDS 35.0%
TCCACGTTAATGTGTGTTAT+TGG - chr4_3:58265850-58265869 Msa0650970:intron 35.0%
TCCAATAACACACATTAACG+TGG + chr4_3:58265854-58265873 None:intergenic 35.0%
TAAATGCATCAGAGTTTGCA+AGG + chr4_3:58266057-58266076 None:intergenic 35.0%
TGCAAACTCTGATGCATTTA+TGG - chr4_3:58266057-58266076 Msa0650970:intron 35.0%
AAACTCTGATGCATTTATGG+TGG - chr4_3:58266060-58266079 Msa0650970:intron 35.0%
CAATTGGAATTTCCATTCCA+AGG + chr4_3:58266096-58266115 None:intergenic 35.0%
GAATACATCTCCATAGTCAA+TGG + chr4_3:58266371-58266390 None:intergenic 35.0%
!! CTCATATTGGTTCTAACTAG+AGG - chr4_3:58266484-58266503 Msa0650970:intron 35.0%
!! ATATTGGTTCTAACTAGAGG+AGG - chr4_3:58266487-58266506 Msa0650970:intron 35.0%
!! TATTGGTTCTAACTAGAGGA+GGG - chr4_3:58266488-58266507 Msa0650970:intron 35.0%
! CAAAATTGGTGGCTAAATAC+AGG - chr4_3:58266518-58266537 Msa0650970:intron 35.0%
TAACGACTGATACCTTTGAT+TGG - chr4_3:58266578-58266597 Msa0650970:intron 35.0%
ATATCAAGCTATGTCTAGCA+GGG + chr4_3:58266621-58266640 None:intergenic 35.0%
AATATCAAGCTATGTCTAGC+AGG + chr4_3:58266622-58266641 None:intergenic 35.0%
GTTTAGATCGATGAGATGAG+TGG + chr4_3:58264060-58264079 None:intergenic 40.0%
! CGTGCAAACGATCTTAGTTT+TGG + chr4_3:58264111-58264130 None:intergenic 40.0%
GCTAGATTCAACTTCTCTCA+TGG - chr4_3:58264194-58264213 Msa0650970:three_prime_UTR 40.0%
TGGTTCTCATGAGTATCATC+AGG - chr4_3:58264214-58264233 Msa0650970:three_prime_UTR 40.0%
TGATAATCTTAGAGCTGCCA+TGG - chr4_3:58264244-58264263 Msa0650970:exon 40.0%
! TACCTTAGTATCGAGCATGA+CGG + chr4_3:58264291-58264310 None:intergenic 40.0%
CTTACATTGCTCATTGCTGT+AGG - chr4_3:58265254-58265273 Msa0650970:intron 40.0%
TACATTGCTCATTGCTGTAG+GGG - chr4_3:58265256-58265275 Msa0650970:intron 40.0%
AGAAATCCAGTTCGAATCTC+AGG + chr4_3:58265282-58265301 None:intergenic 40.0%
GATTCGAACTGGATTTCTCA+AGG - chr4_3:58265284-58265303 Msa0650970:intron 40.0%
CTCCATGAGCTACAAAAAGT+TGG - chr4_3:58265389-58265408 Msa0650970:CDS 40.0%
! AGCCAACTTTTTGTAGCTCA+TGG + chr4_3:58265394-58265413 None:intergenic 40.0%
GTGTGACAAAGAATTGGGTT+TGG - chr4_3:58265479-58265498 Msa0650970:CDS 40.0%
CTTGTCTTTCTCAGTCAAAG+TGG + chr4_3:58265583-58265602 None:intergenic 40.0%
CACTTTGACTGAGAAAGACA+AGG - chr4_3:58265581-58265600 Msa0650970:CDS 40.0%
ACAAGGAAGATATCATGGCT+TGG - chr4_3:58265598-58265617 Msa0650970:CDS 40.0%
CAAGGAAGATATCATGGCTT+GGG - chr4_3:58265599-58265618 Msa0650970:CDS 40.0%
AAGGAAGATATCATGGCTTG+GGG - chr4_3:58265600-58265619 Msa0650970:CDS 40.0%
GCGCTTTCATTTGTTCGAAA+AGG - chr4_3:58265648-58265667 Msa0650970:CDS 40.0%
! TGTTCGAAAAGGTTCTGATC+TGG - chr4_3:58265659-58265678 Msa0650970:CDS 40.0%
CATACATTTGCAAGTTGCAG+AGG + chr4_3:58265990-58266009 None:intergenic 40.0%
CTGCAACTTGCAAATGTATG+TGG - chr4_3:58265990-58266009 Msa0650970:intron 40.0%
AACTTGCAAATGTATGTGGC+AGG - chr4_3:58265994-58266013 Msa0650970:intron 40.0%
GTGCAATATCCAAGGAAAAC+CGG - chr4_3:58266153-58266172 Msa0650970:intron 40.0%
AGTCAATGATCAAATCTCCC+CGG - chr4_3:58266200-58266219 Msa0650970:intron 40.0%
ATTGTACGAACAGCAAGTTC+TGG + chr4_3:58266319-58266338 None:intergenic 40.0%
!! AGAACTTGCTGTTCGTACAA+TGG - chr4_3:58266318-58266337 Msa0650970:intron 40.0%
AAGAAGCTAGACTCTCCAAT+GGG + chr4_3:58266429-58266448 None:intergenic 40.0%
GGAACGACAACAGAAAGAAT+TGG + chr4_3:58266553-58266572 None:intergenic 40.0%
ATCACTGCATGACCAATCAA+AGG + chr4_3:58266593-58266612 None:intergenic 40.0%
TATCAAGCTATGTCTAGCAG+GGG + chr4_3:58266620-58266639 None:intergenic 40.0%
AGACATAGCTTGATATTCCG+AGG - chr4_3:58266625-58266644 Msa0650970:intron 40.0%
GCAGAAGCAGCACTTAATAT+TGG + chr4_3:58266658-58266677 None:intergenic 40.0%
ATACCATCTACAGTCTACGT+TGG - chr4_3:58266900-58266919 Msa0650970:CDS 40.0%
TCAAACAAGCAAGTCTCACG+CGG + chr4_3:58263962-58263981 None:intergenic 45.0%
AACTAAGATCGTTTGCACGC+TGG - chr4_3:58264112-58264131 Msa0650970:three_prime_UTR 45.0%
ACTAAGATCGTTTGCACGCT+GGG - chr4_3:58264113-58264132 Msa0650970:three_prime_UTR 45.0%
ATGGGAATAGACCTAGATGC+GGG + chr4_3:58264140-58264159 None:intergenic 45.0%
CATGGGAATAGACCTAGATG+CGG + chr4_3:58264141-58264160 None:intergenic 45.0%
GAGAATTCCGGTATTCTCCA+TGG + chr4_3:58264264-58264283 None:intergenic 45.0%
TGTGAGAACTCTGCTGTTCT+AGG - chr4_3:58265513-58265532 Msa0650970:CDS 45.0%
! TGGCAGGTTGAAAACCAAGA+AGG - chr4_3:58266010-58266029 Msa0650970:intron 45.0%
! GGCAGGTTGAAAACCAAGAA+GGG - chr4_3:58266011-58266030 Msa0650970:intron 45.0%
! GCAGGTTGAAAACCAAGAAG+GGG - chr4_3:58266012-58266031 Msa0650970:intron 45.0%
CAAAGTTTGCAACCCCTTCT+TGG + chr4_3:58266027-58266046 None:intergenic 45.0%
AACATCAGTAGCTTCAGCTC+TGG + chr4_3:58266230-58266249 None:intergenic 45.0%
TGATGTTGCGAATGCAGTTC+TGG - chr4_3:58266243-58266262 Msa0650970:intron 45.0%
ACAGACTGTGTCATGCTTAG+TGG - chr4_3:58266271-58266290 Msa0650970:intron 45.0%
GAAGAAGCTAGACTCTCCAA+TGG + chr4_3:58266430-58266449 None:intergenic 45.0%
TGCTAGAGCAGCTCTCATAT+TGG - chr4_3:58266471-58266490 Msa0650970:intron 45.0%
AGGAGGGACTACTGCAAAAT+TGG - chr4_3:58266504-58266523 Msa0650970:intron 45.0%
AGGGACTACTGCAAAATTGG+TGG - chr4_3:58266507-58266526 Msa0650970:intron 45.0%
! AAGGTATCAGTCGTTAGCTC+AGG + chr4_3:58266574-58266593 None:intergenic 45.0%
ATTTGCCCTTCAATGTGCCA+AGG - chr4_3:58266717-58266736 Msa0650970:intron 45.0%
TTTGCCCTTCAATGTGCCAA+GGG - chr4_3:58266718-58266737 Msa0650970:intron 45.0%
CTTTCCCTTGGCACATTGAA+GGG + chr4_3:58266725-58266744 None:intergenic 45.0%
CTTCAATGTGCCAAGGGAAA+GGG - chr4_3:58266724-58266743 Msa0650970:intron 45.0%
CATCCAACGTAGACTGTAGA+TGG + chr4_3:58266906-58266925 None:intergenic 45.0%
!!! ATTTTTTATTATTTTTTTCA+TGG - chr4_3:58265754-58265773 Msa0650970:CDS 5.0%
GATCGATGAGATGAGTGGTG+TGG + chr4_3:58264055-58264074 None:intergenic 50.0%
CGCATCTAGGTCTATTCCCA+TGG - chr4_3:58264139-58264158 Msa0650970:three_prime_UTR 50.0%
CGCAAAAGCTTCTCGACCAT+GGG + chr4_3:58264158-58264177 None:intergenic 50.0%
TCGCAAAAGCTTCTCGACCA+TGG + chr4_3:58264159-58264178 None:intergenic 50.0%
AGAGCTGCCATGGAGAATAC+CGG - chr4_3:58264254-58264273 Msa0650970:exon 50.0%
TTGGCTCATGATGTGAAGCC+TGG - chr4_3:58265408-58265427 Msa0650970:CDS 50.0%
TGGCTCATGATGTGAAGCCT+GGG - chr4_3:58265409-58265428 Msa0650970:CDS 50.0%
AGTGTCATACTCTGCGCAGA+TGG - chr4_3:58265432-58265451 Msa0650970:CDS 50.0%
GAACAGCAGAGTTCTCACAG+CGG + chr4_3:58265512-58265531 None:intergenic 50.0%
TAATCTGCCTGGAGTCGTAG+TGG - chr4_3:58265551-58265570 Msa0650970:CDS 50.0%
GATGCATTTATGGTGGCACG+TGG - chr4_3:58266067-58266086 Msa0650970:intron 50.0%
!! CAGTAACGACCGGTTTTCCT+TGG + chr4_3:58266165-58266184 None:intergenic 50.0%
ATCTGAGTCGCAGTAACGAC+CGG + chr4_3:58266175-58266194 None:intergenic 50.0%
CGTTACTGCGACTCAGATGT+TGG - chr4_3:58266177-58266196 Msa0650970:intron 50.0%
! TCAGTAGCTTCAGCTCTGGT+TGG + chr4_3:58266226-58266245 None:intergenic 50.0%
!! GTTGCGAATGCAGTTCTGGA+TGG - chr4_3:58266247-58266266 Msa0650970:intron 50.0%
CTTAGTGGTGAAACTGCTGC+TGG - chr4_3:58266286-58266305 Msa0650970:intron 50.0%
!! GCTGAGAGCACCATTGACTA+TGG - chr4_3:58266358-58266377 Msa0650970:intron 50.0%
! AGACTCTCCAATGGGCTCAT+GGG + chr4_3:58266421-58266440 None:intergenic 50.0%
! TAGACTCTCCAATGGGCTCA+TGG + chr4_3:58266422-58266441 None:intergenic 50.0%
! TTGGTGGCTAAATACAGGCC+AGG - chr4_3:58266523-58266542 Msa0650970:intron 50.0%
ACAGAAAGAATTGGCGTGCC+TGG + chr4_3:58266544-58266563 None:intergenic 50.0%
CCTTTCCCTTGGCACATTGA+AGG + chr4_3:58266726-58266745 None:intergenic 50.0%
CCTTCAATGTGCCAAGGGAA+AGG - chr4_3:58266723-58266742 Msa0650970:intron 50.0%
GGAAAGGGACTCTGCGTTAA+CGG - chr4_3:58266739-58266758 Msa0650970:intron 50.0%
GAAAGGGACTCTGCGTTAAC+GGG - chr4_3:58266740-58266759 Msa0650970:intron 50.0%
AAAGGGACTCTGCGTTAACG+GGG - chr4_3:58266741-58266760 Msa0650970:intron 50.0%
CGTTAACGGGGATTCTGTTG+TGG - chr4_3:58266753-58266772 Msa0650970:intron 50.0%
GAGTGGTGTGGTGGTTCTTC+TGG + chr4_3:58264043-58264062 None:intergenic 55.0%
CGATGAGATGAGTGGTGTGG+TGG + chr4_3:58264052-58264071 None:intergenic 55.0%
! GTCGAGAAGCTTTTGCGAGC+AGG - chr4_3:58264161-58264180 Msa0650970:three_prime_UTR 55.0%
CATGACGGCGCAGAGAATTC+CGG + chr4_3:58264276-58264295 None:intergenic 55.0%
CGCCGTCATGCTCGATACTA+AGG - chr4_3:58264286-58264305 Msa0650970:CDS 55.0%
TAGGGGCCTGAGATTCGAAC+TGG - chr4_3:58265273-58265292 Msa0650970:intron 55.0%
GGAAGATCCACTACGACTCC+AGG + chr4_3:58265561-58265580 None:intergenic 55.0%
! TAGCTTCAGCTCTGGTTGGC+CGG + chr4_3:58266222-58266241 None:intergenic 55.0%
ACCAGTACCCATGAGCCCAT+TGG - chr4_3:58266411-58266430 Msa0650970:intron 55.0%
! TCCAATGGGCTCATGGGTAC+TGG + chr4_3:58266415-58266434 None:intergenic 55.0%
GAGAGCTGCTCTAGCAGAGT+TGG + chr4_3:58266467-58266486 None:intergenic 55.0%
AACGCAGAGTCCCTTTCCCT+TGG + chr4_3:58266737-58266756 None:intergenic 55.0%
! TGTTGTGGTTCTGCATCGCG+TGG - chr4_3:58266768-58266787 Msa0650970:intron 55.0%
! GTTGTGGTTCTGCATCGCGT+GGG - chr4_3:58266769-58266788 Msa0650970:intron 55.0%
GCGCAGAGTATGACACTCCC+AGG + chr4_3:58265429-58265448 None:intergenic 60.0%
ATGGTGGCACGTGGTGACCT+TGG - chr4_3:58266076-58266095 Msa0650970:intron 60.0%
GGCACGTGGTGACCTTGGAA+TGG - chr4_3:58266081-58266100 Msa0650970:intron 60.0%
! AGCTTCAGCTCTGGTTGGCC+GGG + chr4_3:58266221-58266240 None:intergenic 60.0%
! GCTTCAGCTCTGGTTGGCCG+GGG + chr4_3:58266220-58266239 None:intergenic 65.0%
GCACGCTGGGACCCGCATCT+AGG - chr4_3:58264126-58264145 Msa0650970:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 58263922 58267166 58263922 ID=Msa0650970;Name=Msa0650970
chr4_3 mRNA 58263922 58267166 58263922 ID=Msa0650970-mRNA-1;Parent=Msa0650970;Name=Msa0650970-mRNA-1;_AED=0.25;_eAED=0.25;_QI=158|1|1|1|1|1|3|338|500
chr4_3 exon 58263922 58265072 58263922 ID=Msa0650970-mRNA-1:exon:15403;Parent=Msa0650970-mRNA-1
chr4_3 exon 58265351 58265812 58265351 ID=Msa0650970-mRNA-1:exon:15402;Parent=Msa0650970-mRNA-1
chr4_3 exon 58266781 58267166 58266781 ID=Msa0650970-mRNA-1:exon:15401;Parent=Msa0650970-mRNA-1
chr4_3 five_prime_UTR 58267009 58267166 58267009 ID=Msa0650970-mRNA-1:five_prime_utr;Parent=Msa0650970-mRNA-1
chr4_3 CDS 58266781 58267008 58266781 ID=Msa0650970-mRNA-1:cds;Parent=Msa0650970-mRNA-1
chr4_3 CDS 58265351 58265812 58265351 ID=Msa0650970-mRNA-1:cds;Parent=Msa0650970-mRNA-1
chr4_3 CDS 58264260 58265072 58264260 ID=Msa0650970-mRNA-1:cds;Parent=Msa0650970-mRNA-1
chr4_3 three_prime_UTR 58263922 58264259 58263922 ID=Msa0650970-mRNA-1:three_prime_utr;Parent=Msa0650970-mRNA-1
Gene Sequence

>Msa0650970

ATGGCGACGATTGTAGCAGAGAAGAAGCCCAAAACTAAGATCGTTTGCACGCTGGGACCCGCATCTAGGTCTATTCCCATGGTCGAGAAGCTTTTGCGAGCAGGTATGAACGTTGCTAGATTCAACTTCTCTCATGGTTCTCATGAGTATCATCAGGAAACTCTTGATAATCTTAGAGCTGCCATGGAGAATACCGGAATTCTCTGCGCCGTCATGCTCGATACTAAGGGGCCTGAGATTCGAACTGGATTTCTCAAGGACGCCAAACCTATCCAACTGAAACAAGGTAATGAAATAACTATTTCAACTGACTATAGCCTAAAGGGTGATGAGAATACAATCTCCATGAGCTACAAAAAGTTGGCTCATGATGTGAAGCCTGGGAGTGTCATACTCTGCGCAGATGGAACCATATCATTTACAGTCTTATCGTGTGACAAAGAATTGGGTTTGGTTCAAGTCCGCTGTGAGAACTCTGCTGTTCTAGGCGAGAGAAAGAATGTTAATCTGCCTGGAGTCGTAGTGGATCTTCCCACTTTGACTGAGAAAGACAAGGAAGATATCATGGCTTGGGGAGTTCCCAATAAAATTGACATGATTGCGCTTTCATTTGTTCGAAAAGGTTCTGATCTGGTACAAGTTCGAAAACTTCTAGGACACCATGCTAAGAACATTCTTCTCATGTCAAAGGTTGAAAACCAAGAAGGGGTTGCAAACTTTGATGATATCCTTGCAAACTCTGATGCATTTATGGTGGCACGTGGTGACCTTGGAATGGAAATTCCAATTGAGAAAATATTTCTCGCACAGAAAGTGATGATTTATAAGTGCAATATCCAAGGAAAACCGGTCGTTACTGCGACTCAGATGTTGGAGTCAATGATCAAATCTCCCCGGCCAACCAGAGCTGAAGCTACTGATGTTGCGAATGCAGTTCTGGATGGAACAGACTGTGTCATGCTTAGTGGTGAAACTGCTGCTGGAGCTTATCCAGAACTTGCTGTTCGTACAATGGCTAAAATATGTGTTGAAGCTGAGAGCACCATTGACTATGGAGATGTATTCAAAAGGATAATGGAGCACTCACCAGTACCCATGAGCCCATTGGAGAGTCTAGCTTCTTCTGCTGTTAGAACTGCCAACTCTGCTAGAGCAGCTCTCATATTGGTTCTAACTAGAGGAGGGACTACTGCAAAATTGGTGGCTAAATACAGGCCAGGCACGCCAATTCTTTCTGTTGTCGTTCCTGAGCTAACGACTGATACCTTTGATTGGTCATGCAGTGATGAGTCCCCTGCTAGACATAGCTTGATATTCCGAGGATTGATTCCAATATTAAGTGCTGCTTCTGCTAGAGCTTCTCATGCAGAAACAACAGAAGATGCAATAGAATTTGCCCTTCAATGTGCCAAGGGAAAGGGACTCTGCGTTAACGGGGATTCTGTTGTGGTTCTGCATCGCGTGGGTACCGCATCAATCATCAAAATCTTGACCGTGAAATGA
Protein sequence

>Msa0650970

MATIVAEKKPKTKIVCTLGPASRSIPMVEKLLRAGMNVARFNFSHGSHEYHQETLDNLRAAMENTGILCAVMLDTKGPEIRTGFLKDAKPIQLKQGNEITISTDYSLKGDENTISMSYKKLAHDVKPGSVILCADGTISFTVLSCDKELGLVQVRCENSAVLGERKNVNLPGVVVDLPTLTEKDKEDIMAWGVPNKIDMIALSFVRKGSDLVQVRKLLGHHAKNILLMSKVENQEGVANFDDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTIDYGDVFKRIMEHSPVPMSPLESLASSAVRTANSARAALILVLTRGGTTAKLVAKYRPGTPILSVVVPELTTDTFDWSCSDESPARHSLIFRGLIPILSAASARASHAETTEDAIEFALQCAKGKGLCVNGDSVVVLHRVGTASIIKILTVK*