AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0619840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0619840 MtrunA17_Chr4g0043301 99.200 500 4 0 1 500 1 500 0.0 1022
Msa0619840 MtrunA17_Chr7g0263711 93.600 500 32 0 1 500 1 500 0.0 969
Msa0619840 MtrunA17_Chr4g0043311 89.336 497 53 0 4 500 1 497 0.0 926
Msa0619840 MtrunA17_Chr6g0448901 78.819 491 104 0 10 500 20 510 0.0 818
Msa0619840 MtrunA17_Chr7g0265591 42.485 499 275 7 12 500 30 526 1.31e-128 384
Msa0619840 MtrunA17_Chr1g0197911 41.748 515 282 9 1 500 14 525 2.63e-127 380
Msa0619840 MtrunA17_Chr1g0179541 41.517 501 263 7 12 500 30 512 6.11e-122 367
Msa0619840 MtrunA17_Chr5g0400361 38.780 410 237 8 6 412 73 471 4.73e-89 283
Msa0619840 MtrunA17_Chr6g0463101 35.787 489 265 12 9 486 104 554 4.68e-84 270
Msa0619840 MtrunA17_Chr1g0202291 35.174 489 268 12 9 486 102 552 1.54e-83 269
Msa0619840 MtrunA17_Chr1g0188101 31.600 500 285 11 11 485 90 557 5.75e-71 236
Msa0619840 MtrunA17_Chr1g0206791 32.383 491 294 11 11 488 17 482 2.11e-69 230
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0619840 AT5G56350.1 89.271 494 53 0 7 500 5 498 0.0 908
Msa0619840 AT4G26390.1 83.603 494 81 0 7 500 4 497 0.0 867
Msa0619840 AT5G08570.1 79.022 491 103 0 10 500 20 510 0.0 821
Msa0619840 AT5G08570.2 79.022 491 103 0 10 500 20 510 0.0 821
Msa0619840 AT5G08570.3 79.022 491 103 0 10 500 20 510 0.0 821
Msa0619840 AT5G63680.3 78.702 493 105 0 8 500 18 510 0.0 818
Msa0619840 AT5G63680.1 78.702 493 105 0 8 500 18 510 0.0 818
Msa0619840 AT5G63680.2 78.702 493 105 0 8 500 18 510 0.0 818
Msa0619840 AT3G04050.1 73.790 496 126 3 9 500 15 510 0.0 758
Msa0619840 AT3G25960.1 72.470 494 123 4 9 500 15 497 0.0 728
Msa0619840 AT3G55650.1 71.169 496 139 3 9 500 15 510 0.0 726
Msa0619840 AT3G55810.1 67.944 496 137 4 9 500 15 492 0.0 673
Msa0619840 AT2G36580.1 43.487 499 270 7 12 500 30 526 3.03e-130 389
Msa0619840 AT3G52990.1 43.287 499 271 7 12 500 30 526 2.82e-129 386
Msa0619840 AT3G52990.2 42.526 475 261 7 36 500 1 473 3.76e-120 361
Msa0619840 AT5G52920.1 36.179 492 272 13 11 495 111 567 1.42e-84 272
Msa0619840 AT1G32440.1 39.320 412 229 10 7 412 96 492 4.21e-81 263
Msa0619840 AT3G22960.1 31.717 495 289 12 11 485 118 583 3.25e-70 235
Msa0619840 AT3G49160.3 31.618 272 159 9 73 322 325 591 1.55e-21 98.6
Msa0619840 AT3G49160.1 31.618 272 159 9 73 322 413 679 1.75e-21 98.6

Find 136 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAACTCTGATGCATTTA+TGG 0.128456 4_2:+71172696 Msa0619840:CDS
AAGCGGTTTCAGTTGTTTAT+TGG 0.237023 4_2:+71170688 Msa0619840:five_prime_UTR
CAAAGTTTGCAACCCCTTCT+TGG 0.251729 4_2:-71172663 None:intergenic
CGTGCAAACGATCTTAGTTT+TGG 0.261618 4_2:-71170804 None:intergenic
TCTCAACAATAACAATAATA+AGG 0.268598 4_2:-71173636 None:intergenic
GAGTGGTGTGGTGGTTCTTC+TGG 0.285238 4_2:-71170736 None:intergenic
AGGAGGGACTACTGCAAAAT+TGG 0.314986 4_2:+71173143 Msa0619840:CDS
GTACAAGTTCGAAAACTTCT+AGG 0.320879 4_2:+71172320 Msa0619840:CDS
TGTGAGAACTCTGCTGTTCT+AGG 0.329692 4_2:+71172152 Msa0619840:CDS
GCAGAAGCAGCACTTAATAT+TGG 0.330688 4_2:-71173294 None:intergenic
TGATGTTGCGAATGCAGTTC+TGG 0.344149 4_2:+71172882 Msa0619840:CDS
GAAAGGGACTCTGCGTTAAC+GGG 0.361244 4_2:+71173379 Msa0619840:CDS
CTTATCGTGTGACAAAGAAT+TGG 0.361638 4_2:+71172112 Msa0619840:CDS
TTGTATTCTCATCACCCTTT+AGG 0.370256 4_2:-71172004 None:intergenic
CGATAAGACTGTAAATGATA+TGG 0.371207 4_2:-71172096 None:intergenic
AGCTTCAGCTCTGGTTGGCC+GGG 0.390347 4_2:-71172857 None:intergenic
TAGCTTCAGCTCTGGTTGGC+CGG 0.391145 4_2:-71172858 None:intergenic
CAAGGAAGATATCATGGCTT+GGG 0.397205 4_2:+71172238 Msa0619840:CDS
CCTTTCCCTTGGCACATTGA+AGG 0.403085 4_2:-71173362 None:intergenic
GCTGAGAGCACCATTGACTA+TGG 0.403578 4_2:+71172997 Msa0619840:CDS
GGAAAGGGACTCTGCGTTAA+CGG 0.404260 4_2:+71173378 Msa0619840:CDS
CGCCGTCATGCTCGATACTA+AGG 0.406356 4_2:+71170982 Msa0619840:CDS
TCAACTGACTATAGCCTAAA+GGG 0.420476 4_2:+71171990 Msa0619840:CDS
CATGACGGCGCAGAGAATTC+CGG 0.420747 4_2:-71170969 None:intergenic
CATCCAACGTAGACTGTAGA+TGG 0.432323 4_2:-71173547 None:intergenic
TTGTTTCAGTTGGATAGGTT+TGG 0.435015 4_2:-71171949 None:intergenic
TGCTAGAGCAGCTCTCATAT+TGG 0.437951 4_2:+71173110 Msa0619840:CDS
AGATGTATTCAAAAGGATAA+TGG 0.438677 4_2:+71173020 Msa0619840:CDS
GGAACGACAACAGAAAGAAT+TGG 0.441249 4_2:-71173189 None:intergenic
GCACGCTGGGACCCGCATCT+AGG 0.445382 4_2:+71170822 Msa0619840:CDS
TTATCGTGTGACAAAGAATT+GGG 0.446712 4_2:+71172113 Msa0619840:CDS
ACAGAAAGAATTGGCGTGCC+TGG 0.449963 4_2:-71173180 None:intergenic
TATCATGATTGAGAAGTGTA+TGG 0.450523 4_2:+71173783 Msa0619840:three_prime_UTR
GCGCTTTCATTTGTTCGAAA+AGG 0.450686 4_2:+71172287 Msa0619840:CDS
CAAAATTGGTGGCTAAATAC+AGG 0.450918 4_2:+71173157 Msa0619840:CDS
TTTCATTACCTTGTTTCAGT+TGG 0.451778 4_2:-71171959 None:intergenic
TTACCTTGTTTCAGTTGGAT+AGG 0.452533 4_2:-71171954 None:intergenic
TGTTCGAAAAGGTTCTGATC+TGG 0.452582 4_2:+71172298 Msa0619840:CDS
TCCAATGGGCTCATGGGTAC+TGG 0.457116 4_2:-71173051 None:intergenic
TAACGACTGATACCTTTGAT+TGG 0.457143 4_2:+71173217 Msa0619840:CDS
AGAAATCCAGTTCGAATCTC+AGG 0.458723 4_2:-71171918 None:intergenic
ACTAAGATCGTTTGCACGCT+GGG 0.459837 4_2:+71170809 Msa0619840:CDS
GAGAGCTGCTCTAGCAGAGT+TGG 0.462449 4_2:-71173103 None:intergenic
CTTTCCCTTGGCACATTGAA+GGG 0.463400 4_2:-71173361 None:intergenic
ATTGTACGAACAGCAAGTTC+TGG 0.463793 4_2:-71172955 None:intergenic
GAATACATCTCCATAGTCAA+TGG 0.466000 4_2:-71173007 None:intergenic
TAACAATAATAAGGTAATAG+TGG 0.466373 4_2:-71173627 None:intergenic
AACGCAGAGTCCCTTTCCCT+TGG 0.468309 4_2:-71173373 None:intergenic
CGCAAAAGCTTCTCGACCAT+GGG 0.471680 4_2:-71170851 None:intergenic
TAGACTCTCCAATGGGCTCA+TGG 0.478114 4_2:-71173058 None:intergenic
TTGGCTCATGATGTGAAGCC+TGG 0.480964 4_2:+71172047 Msa0619840:CDS
CAATTGGAATTTCCATTCCA+AGG 0.480988 4_2:-71172732 None:intergenic
GCTAGATTCAACTTCTCTCA+TGG 0.485672 4_2:+71170890 Msa0619840:CDS
GTGTGACAAAGAATTGGGTT+TGG 0.488491 4_2:+71172118 Msa0619840:CDS
GTGCAATATCCAAGGAAAAC+CGG 0.489545 4_2:+71172792 Msa0619840:CDS
AGAAAGAATGTTAATCTGCC+TGG 0.492316 4_2:+71172179 Msa0619840:CDS
AATATCAAGCTATGTCTAGC+AGG 0.493341 4_2:-71173258 None:intergenic
TAGGGGCCTGAGATTCGAAC+TGG 0.499972 4_2:+71171912 Msa0619840:intron
CTTCAATGTGCCAAGGGAAA+GGG 0.500302 4_2:+71173363 Msa0619840:CDS
TTCAACTGACTATAGCCTAA+AGG 0.500418 4_2:+71171989 Msa0619840:CDS
CGTTAACGGGGATTCTGTTG+TGG 0.503390 4_2:+71173392 Msa0619840:CDS
GCTTCAGCTCTGGTTGGCCG+GGG 0.507929 4_2:-71172856 None:intergenic
CGTTACTGCGACTCAGATGT+TGG 0.507988 4_2:+71172816 Msa0619840:CDS
ATCTGAGTCGCAGTAACGAC+CGG 0.509493 4_2:-71172811 None:intergenic
GAAAGACAAGGAAGATATCA+TGG 0.513280 4_2:+71172232 Msa0619840:CDS
TGGTTCTCATGAGTATCATC+AGG 0.514740 4_2:+71170910 Msa0619840:CDS
CCTTCAATGTGCCAAGGGAA+AGG 0.523286 4_2:+71173362 Msa0619840:CDS
ACAGACTGTGTCATGCTTAG+TGG 0.525623 4_2:+71172910 Msa0619840:CDS
ACAAGGAAGATATCATGGCT+TGG 0.526189 4_2:+71172237 Msa0619840:CDS
AAGGTATCAGTCGTTAGCTC+AGG 0.530961 4_2:-71173210 None:intergenic
CGCATCTAGGTCTATTCCCA+TGG 0.531643 4_2:+71170835 Msa0619840:CDS
TGATAATCTTAGAGCTGCCA+TGG 0.531806 4_2:+71170940 Msa0619840:CDS
AGTGTCATACTCTGCGCAGA+TGG 0.532331 4_2:+71172071 Msa0619840:CDS
ACTATGGAGATGTATTCAAA+AGG 0.538144 4_2:+71173013 Msa0619840:CDS
GGAAGATCCACTACGACTCC+AGG 0.538876 4_2:-71172197 None:intergenic
CTTGTCTTTCTCAGTCAAAG+TGG 0.540820 4_2:-71172219 None:intergenic
AACTAAGATCGTTTGCACGC+TGG 0.542989 4_2:+71170808 Msa0619840:CDS
TCGCAAAAGCTTCTCGACCA+TGG 0.546576 4_2:-71170852 None:intergenic
CTTAGTGGTGAAACTGCTGC+TGG 0.547208 4_2:+71172925 Msa0619840:CDS
AACATCAGTAGCTTCAGCTC+TGG 0.547477 4_2:-71172866 None:intergenic
GGCAGGTTGAAAACCAAGAA+GGG 0.548016 4_2:+71172650 Msa0619840:intron
GAACATTCTTCTCATGTCAA+AGG 0.556045 4_2:+71172355 Msa0619840:CDS
AGACTCTCCAATGGGCTCAT+GGG 0.558290 4_2:-71173057 None:intergenic
AGGGACTACTGCAAAATTGG+TGG 0.560616 4_2:+71173146 Msa0619840:CDS
CTCATATTGGTTCTAACTAG+AGG 0.560702 4_2:+71173123 Msa0619840:CDS
TTAATATTGGAATCAATCCT+CGG 0.562918 4_2:-71173281 None:intergenic
AGAGCTGCCATGGAGAATAC+CGG 0.568658 4_2:+71170950 Msa0619840:CDS
ACCAGTACCCATGAGCCCAT+TGG 0.569139 4_2:+71173050 Msa0619840:CDS
TTTGAGAATATCTATATAAG+CGG 0.569632 4_2:+71170671 Msa0619840:five_prime_UTR
TTGTCTTTCTCAGTCAAAGT+GGG 0.570627 4_2:-71172218 None:intergenic
TTTAATGGGAGATCATTTCA+CGG 0.575483 4_2:-71173457 None:intergenic
GGCACGTGGTGACCTTGGAA+TGG 0.579482 4_2:+71172720 Msa0619840:CDS
AAGAAGCTAGACTCTCCAAT+GGG 0.579536 4_2:-71173065 None:intergenic
TTGGTGGCTAAATACAGGCC+AGG 0.581932 4_2:+71173162 Msa0619840:CDS
TGGCAGGTTGAAAACCAAGA+AGG 0.582341 4_2:+71172649 Msa0619840:intron
ATATCAAGCTATGTCTAGCA+GGG 0.582962 4_2:-71173257 None:intergenic
TCAGTAGCTTCAGCTCTGGT+TGG 0.584103 4_2:-71172862 None:intergenic
TGTTGTGGTTCTGCATCGCG+TGG 0.586595 4_2:+71173407 Msa0619840:CDS
GATTCGAACTGGATTTCTCA+AGG 0.588567 4_2:+71171923 Msa0619840:CDS
ATTTGCCCTTCAATGTGCCA+AGG 0.591498 4_2:+71173356 Msa0619840:CDS
TAAATGCATCAGAGTTTGCA+AGG 0.592927 4_2:-71172693 None:intergenic
ATGGTGGCACGTGGTGACCT+TGG 0.597261 4_2:+71172715 Msa0619840:CDS
ATTTATAAGTGCAATATCCA+AGG 0.598187 4_2:+71172784 Msa0619840:CDS
AGAACTTGCTGTTCGTACAA+TGG 0.602563 4_2:+71172957 Msa0619840:CDS
AGACATAGCTTGATATTCCG+AGG 0.602907 4_2:+71173264 Msa0619840:CDS
GCGCAGAGTATGACACTCCC+AGG 0.606193 4_2:-71172065 None:intergenic
ATATTGGTTCTAACTAGAGG+AGG 0.607238 4_2:+71173126 Msa0619840:CDS
CACTTTGACTGAGAAAGACA+AGG 0.607462 4_2:+71172220 Msa0619840:CDS
TGAGAAGAATGTTCTTAGCA+TGG 0.609210 4_2:-71172346 None:intergenic
TTTGCCCTTCAATGTGCCAA+GGG 0.609763 4_2:+71173357 Msa0619840:CDS
GTTGCGAATGCAGTTCTGGA+TGG 0.610844 4_2:+71172886 Msa0619840:CDS
GAGAATTCCGGTATTCTCCA+TGG 0.611587 4_2:-71170957 None:intergenic
ATCACTGCATGACCAATCAA+AGG 0.619997 4_2:-71173229 None:intergenic
GAAGAAGCTAGACTCTCCAA+TGG 0.622268 4_2:-71173066 None:intergenic
TGGCTCATGATGTGAAGCCT+GGG 0.622471 4_2:+71172048 Msa0619840:CDS
TAATCTGCCTGGAGTCGTAG+TGG 0.623976 4_2:+71172190 Msa0619840:CDS
ATGGGAATAGACCTAGATGC+GGG 0.628393 4_2:-71170833 None:intergenic
TATTGGTTCTAACTAGAGGA+GGG 0.629589 4_2:+71173127 Msa0619840:CDS
AAACCTATCCAACTGAAACA+AGG 0.639584 4_2:+71171951 Msa0619840:CDS
AAGGAAGATATCATGGCTTG+GGG 0.639801 4_2:+71172239 Msa0619840:CDS
TCATCTCATCGATCTAAACA+TGG 0.643002 4_2:+71170757 Msa0619840:exon
CGATGAGATGAGTGGTGTGG+TGG 0.644965 4_2:-71170745 None:intergenic
AGTCAATGATCAAATCTCCC+CGG 0.646746 4_2:+71172839 Msa0619840:CDS
AAAGGGACTCTGCGTTAACG+GGG 0.647503 4_2:+71173380 Msa0619840:CDS
GTTGTGGTTCTGCATCGCGT+GGG 0.653541 4_2:+71173408 Msa0619840:CDS
ATACCATCTACAGTCTACGT+TGG 0.672189 4_2:+71173544 Msa0619840:three_prime_UTR
GCAGGTTGAAAACCAAGAAG+GGG 0.675723 4_2:+71172651 Msa0619840:intron
TCAAACAAGCAAGTCTCACG+CGG 0.683267 4_2:-71170653 None:intergenic
TACCTTAGTATCGAGCATGA+CGG 0.683613 4_2:-71170984 None:intergenic
CATGGGAATAGACCTAGATG+CGG 0.689751 4_2:-71170834 None:intergenic
GATCGATGAGATGAGTGGTG+TGG 0.702627 4_2:-71170748 None:intergenic
AAACTCTGATGCATTTATGG+TGG 0.705208 4_2:+71172699 Msa0619840:CDS
TATCAAGCTATGTCTAGCAG+GGG 0.708675 4_2:-71173256 None:intergenic
GTTTAGATCGATGAGATGAG+TGG 0.731788 4_2:-71170753 None:intergenic
GATGCATTTATGGTGGCACG+TGG 0.742236 4_2:+71172706 Msa0619840:CDS
GAACAGCAGAGTTCTCACAG+CGG 0.862341 4_2:-71172148 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAAAAAATAAAAAA+AGG - chr4_2:71171015-71171034 None:intergenic 0.0%
!!! ATAAAAAATCACATTTTAAT+GGG - chr4_2:71173474-71173493 None:intergenic 10.0%
!!! CTGAAAATAATTTTTGTATT+TGG + chr4_2:71171417-71171436 Msa0619840:intron 15.0%
!! TACTTGATTCTTAATATTTA+TGG + chr4_2:71171738-71171757 Msa0619840:intron 15.0%
!!! GATAAAAAATCACATTTTAA+TGG - chr4_2:71173475-71173494 None:intergenic 15.0%
!!! TGTTTTTGATTATTATTTTG+CGG + chr4_2:71173733-71173752 Msa0619840:three_prime_UTR 15.0%
!!! CTAGTTTTGTTGAATAATTT+TGG + chr4_2:71170629-71170648 Msa0619840:five_prime_UTR 20.0%
!! TTTGAGAATATCTATATAAG+CGG + chr4_2:71170671-71170690 Msa0619840:five_prime_UTR 20.0%
!! AATTCTTATCCATACATATA+AGG - chr4_2:71171180-71171199 None:intergenic 20.0%
!! TAAAAGAACTATAGTCTATT+TGG - chr4_2:71171670-71171689 None:intergenic 20.0%
!! ATTAAGAATCAAGTAAAAAC+CGG - chr4_2:71171733-71171752 None:intergenic 20.0%
!! TAACAATAATAAGGTAATAG+TGG - chr4_2:71173630-71173649 None:intergenic 20.0%
!! TCTCAACAATAACAATAATA+AGG - chr4_2:71173639-71173658 None:intergenic 20.0%
!!! AGATTATATGCATGAATTTT+TGG + chr4_2:71173686-71173705 Msa0619840:three_prime_UTR 20.0%
!!! TGATTTTGACCTTATATGTA+TGG + chr4_2:71171168-71171187 Msa0619840:intron 25.0%
! TGTTAGATTAGGTTATGTTT+GGG + chr4_2:71171254-71171273 Msa0619840:intron 25.0%
!! TTTTATAAGATGTCTTAGAG+AGG + chr4_2:71171381-71171400 Msa0619840:intron 25.0%
!!! TATGGTTTAGAACCTTATTT+TGG + chr4_2:71171756-71171775 Msa0619840:intron 25.0%
! TTACAATACTCACCAAAATA+AGG - chr4_2:71171771-71171790 None:intergenic 25.0%
!!! TATTAAGAGAGTTTTAGTTG+CGG - chr4_2:71171816-71171835 None:intergenic 25.0%
!! GCAATCATGTCAATTTTATT+GGG - chr4_2:71172269-71172288 None:intergenic 25.0%
! AAGAGATAGAAAAAATCAGT+AGG - chr4_2:71172567-71172586 None:intergenic 25.0%
! ATTTATAAGTGCAATATCCA+AGG + chr4_2:71172784-71172803 Msa0619840:CDS 25.0%
! AGATGTATTCAAAAGGATAA+TGG + chr4_2:71173020-71173039 Msa0619840:CDS 25.0%
! TTAATATTGGAATCAATCCT+CGG - chr4_2:71173284-71173303 None:intergenic 25.0%
!!! GTGATTTTTTATCTGCTTTT+TGG + chr4_2:71173482-71173501 Msa0619840:three_prime_UTR 25.0%
!! ACATCAATTGATGCTTTTAT+TGG + chr4_2:71173574-71173593 Msa0619840:three_prime_UTR 25.0%
!! CATCAATTGATGCTTTTATT+GGG + chr4_2:71173575-71173594 Msa0619840:three_prime_UTR 25.0%
!! ATCAATTGATGCTTTTATTG+GGG + chr4_2:71173576-71173595 Msa0619840:three_prime_UTR 25.0%
! ATGCAAATACGTGTAAAAAA+GGG - chr4_2:71173606-71173625 None:intergenic 25.0%
! TATGCAAATACGTGTAAAAA+AGG - chr4_2:71173607-71173626 None:intergenic 25.0%
!!! TTTTTGGTACTCAAAAATGT+AGG + chr4_2:71173702-71173721 Msa0619840:three_prime_UTR 25.0%
CGGTAAAAGTCACTATTAAT+CGG - chr4_2:71171217-71171236 None:intergenic 30.0%
ACAGTTCAAACATGTATTGA+CGG - chr4_2:71171237-71171256 None:intergenic 30.0%
CATGTTTGAACTGTTAGATT+AGG + chr4_2:71171243-71171262 Msa0619840:intron 30.0%
CTGTTAGATTAGGTTATGTT+TGG + chr4_2:71171253-71171272 Msa0619840:intron 30.0%
TGTTTCATGCAAGTTATTAG+CGG + chr4_2:71171357-71171376 Msa0619840:intron 30.0%
!! ATAAAAGCATCTTCAATGTC+CGG + chr4_2:71171711-71171730 Msa0619840:intron 30.0%
! TTTCATTACCTTGTTTCAGT+TGG - chr4_2:71171962-71171981 None:intergenic 30.0%
CGATAAGACTGTAAATGATA+TGG - chr4_2:71172099-71172118 None:intergenic 30.0%
TTATCGTGTGACAAAGAATT+GGG + chr4_2:71172113-71172132 Msa0619840:CDS 30.0%
! CGCAATCATGTCAATTTTAT+TGG - chr4_2:71172270-71172289 None:intergenic 30.0%
! GCGAGAAATATTTTCTCAAT+TGG - chr4_2:71172751-71172770 None:intergenic 30.0%
ACTATGGAGATGTATTCAAA+AGG + chr4_2:71173013-71173032 Msa0619840:CDS 30.0%
!! CAAGATTTTGATGATTGATG+CGG - chr4_2:71173436-71173455 None:intergenic 30.0%
TTTAATGGGAGATCATTTCA+CGG - chr4_2:71173460-71173479 None:intergenic 30.0%
!! TTTGGCGAAGTATTTTATGA+TGG + chr4_2:71173500-71173519 Msa0619840:three_prime_UTR 30.0%
! TATCATGATTGAGAAGTGTA+TGG + chr4_2:71173783-71173802 Msa0619840:three_prime_UTR 30.0%
AAGCGGTTTCAGTTGTTTAT+TGG + chr4_2:71170688-71170707 Msa0619840:five_prime_UTR 35.0%
TCATCTCATCGATCTAAACA+TGG + chr4_2:71170757-71170776 Msa0619840:exon 35.0%
!! GTGCAAACGATCTTAGTTTT+GGG - chr4_2:71170806-71170825 None:intergenic 35.0%
AAGATGTCTTAGAGAGGTTA+TGG + chr4_2:71171387-71171406 Msa0619840:intron 35.0%
! TTGGTGAGTATTGTAATTCG+AGG + chr4_2:71171775-71171794 Msa0619840:intron 35.0%
! TGGTGAGTATTGTAATTCGA+GGG + chr4_2:71171776-71171795 Msa0619840:intron 35.0%
ATGAAGCAACTTGAAAATGC+TGG - chr4_2:71171847-71171866 None:intergenic 35.0%
TTACATTGCTCATTGCTGTA+GGG + chr4_2:71171894-71171913 Msa0619840:intron 35.0%
TTGTTTCAGTTGGATAGGTT+TGG - chr4_2:71171952-71171971 None:intergenic 35.0%
AAACCTATCCAACTGAAACA+AGG + chr4_2:71171951-71171970 Msa0619840:CDS 35.0%
! TTACCTTGTTTCAGTTGGAT+AGG - chr4_2:71171957-71171976 None:intergenic 35.0%
TTCAACTGACTATAGCCTAA+AGG + chr4_2:71171989-71172008 Msa0619840:CDS 35.0%
TCAACTGACTATAGCCTAAA+GGG + chr4_2:71171990-71172009 Msa0619840:CDS 35.0%
TTGTATTCTCATCACCCTTT+AGG - chr4_2:71172007-71172026 None:intergenic 35.0%
CTTATCGTGTGACAAAGAAT+TGG + chr4_2:71172112-71172131 Msa0619840:CDS 35.0%
AGAAAGAATGTTAATCTGCC+TGG + chr4_2:71172179-71172198 Msa0619840:CDS 35.0%
TTGTCTTTCTCAGTCAAAGT+GGG - chr4_2:71172221-71172240 None:intergenic 35.0%
GAAAGACAAGGAAGATATCA+TGG + chr4_2:71172232-71172251 Msa0619840:CDS 35.0%
GTACAAGTTCGAAAACTTCT+AGG + chr4_2:71172320-71172339 Msa0619840:CDS 35.0%
TGAGAAGAATGTTCTTAGCA+TGG - chr4_2:71172349-71172368 None:intergenic 35.0%
GAACATTCTTCTCATGTCAA+AGG + chr4_2:71172355-71172374 Msa0619840:CDS 35.0%
TCCACGTTAATGTGTGTTAT+TGG + chr4_2:71172489-71172508 Msa0619840:intron 35.0%
TCCAATAACACACATTAACG+TGG - chr4_2:71172493-71172512 None:intergenic 35.0%
TAAATGCATCAGAGTTTGCA+AGG - chr4_2:71172696-71172715 None:intergenic 35.0%
TGCAAACTCTGATGCATTTA+TGG + chr4_2:71172696-71172715 Msa0619840:CDS 35.0%
AAACTCTGATGCATTTATGG+TGG + chr4_2:71172699-71172718 Msa0619840:CDS 35.0%
CAATTGGAATTTCCATTCCA+AGG - chr4_2:71172735-71172754 None:intergenic 35.0%
GAATACATCTCCATAGTCAA+TGG - chr4_2:71173010-71173029 None:intergenic 35.0%
!! CTCATATTGGTTCTAACTAG+AGG + chr4_2:71173123-71173142 Msa0619840:CDS 35.0%
!! ATATTGGTTCTAACTAGAGG+AGG + chr4_2:71173126-71173145 Msa0619840:CDS 35.0%
!! TATTGGTTCTAACTAGAGGA+GGG + chr4_2:71173127-71173146 Msa0619840:CDS 35.0%
! CAAAATTGGTGGCTAAATAC+AGG + chr4_2:71173157-71173176 Msa0619840:CDS 35.0%
TAACGACTGATACCTTTGAT+TGG + chr4_2:71173217-71173236 Msa0619840:CDS 35.0%
ATATCAAGCTATGTCTAGCA+GGG - chr4_2:71173260-71173279 None:intergenic 35.0%
AATATCAAGCTATGTCTAGC+AGG - chr4_2:71173261-71173280 None:intergenic 35.0%
GTTTAGATCGATGAGATGAG+TGG - chr4_2:71170756-71170775 None:intergenic 40.0%
! CGTGCAAACGATCTTAGTTT+TGG - chr4_2:71170807-71170826 None:intergenic 40.0%
GCTAGATTCAACTTCTCTCA+TGG + chr4_2:71170890-71170909 Msa0619840:CDS 40.0%
TGGTTCTCATGAGTATCATC+AGG + chr4_2:71170910-71170929 Msa0619840:CDS 40.0%
TGATAATCTTAGAGCTGCCA+TGG + chr4_2:71170940-71170959 Msa0619840:CDS 40.0%
! TACCTTAGTATCGAGCATGA+CGG - chr4_2:71170987-71171006 None:intergenic 40.0%
CTTACATTGCTCATTGCTGT+AGG + chr4_2:71171893-71171912 Msa0619840:intron 40.0%
TACATTGCTCATTGCTGTAG+GGG + chr4_2:71171895-71171914 Msa0619840:intron 40.0%
AGAAATCCAGTTCGAATCTC+AGG - chr4_2:71171921-71171940 None:intergenic 40.0%
GATTCGAACTGGATTTCTCA+AGG + chr4_2:71171923-71171942 Msa0619840:CDS 40.0%
CTCCATGAGCTACAAAAAGT+TGG + chr4_2:71172028-71172047 Msa0619840:CDS 40.0%
! AGCCAACTTTTTGTAGCTCA+TGG - chr4_2:71172033-71172052 None:intergenic 40.0%
GTGTGACAAAGAATTGGGTT+TGG + chr4_2:71172118-71172137 Msa0619840:CDS 40.0%
CTTGTCTTTCTCAGTCAAAG+TGG - chr4_2:71172222-71172241 None:intergenic 40.0%
CACTTTGACTGAGAAAGACA+AGG + chr4_2:71172220-71172239 Msa0619840:CDS 40.0%
ACAAGGAAGATATCATGGCT+TGG + chr4_2:71172237-71172256 Msa0619840:CDS 40.0%
CAAGGAAGATATCATGGCTT+GGG + chr4_2:71172238-71172257 Msa0619840:CDS 40.0%
AAGGAAGATATCATGGCTTG+GGG + chr4_2:71172239-71172258 Msa0619840:CDS 40.0%
GCGCTTTCATTTGTTCGAAA+AGG + chr4_2:71172287-71172306 Msa0619840:CDS 40.0%
! TGTTCGAAAAGGTTCTGATC+TGG + chr4_2:71172298-71172317 Msa0619840:CDS 40.0%
CATACATTTGCAAGTTGCAG+AGG - chr4_2:71172629-71172648 None:intergenic 40.0%
CTGCAACTTGCAAATGTATG+TGG + chr4_2:71172629-71172648 Msa0619840:intron 40.0%
AACTTGCAAATGTATGTGGC+AGG + chr4_2:71172633-71172652 Msa0619840:intron 40.0%
GTGCAATATCCAAGGAAAAC+CGG + chr4_2:71172792-71172811 Msa0619840:CDS 40.0%
AGTCAATGATCAAATCTCCC+CGG + chr4_2:71172839-71172858 Msa0619840:CDS 40.0%
ATTGTACGAACAGCAAGTTC+TGG - chr4_2:71172958-71172977 None:intergenic 40.0%
!! AGAACTTGCTGTTCGTACAA+TGG + chr4_2:71172957-71172976 Msa0619840:CDS 40.0%
AAGAAGCTAGACTCTCCAAT+GGG - chr4_2:71173068-71173087 None:intergenic 40.0%
GGAACGACAACAGAAAGAAT+TGG - chr4_2:71173192-71173211 None:intergenic 40.0%
ATCACTGCATGACCAATCAA+AGG - chr4_2:71173232-71173251 None:intergenic 40.0%
TATCAAGCTATGTCTAGCAG+GGG - chr4_2:71173259-71173278 None:intergenic 40.0%
AGACATAGCTTGATATTCCG+AGG + chr4_2:71173264-71173283 Msa0619840:CDS 40.0%
GCAGAAGCAGCACTTAATAT+TGG - chr4_2:71173297-71173316 None:intergenic 40.0%
ATACCATCTACAGTCTACGT+TGG + chr4_2:71173544-71173563 Msa0619840:three_prime_UTR 40.0%
TCAAACAAGCAAGTCTCACG+CGG - chr4_2:71170656-71170675 None:intergenic 45.0%
AACTAAGATCGTTTGCACGC+TGG + chr4_2:71170808-71170827 Msa0619840:CDS 45.0%
ACTAAGATCGTTTGCACGCT+GGG + chr4_2:71170809-71170828 Msa0619840:CDS 45.0%
ATGGGAATAGACCTAGATGC+GGG - chr4_2:71170836-71170855 None:intergenic 45.0%
CATGGGAATAGACCTAGATG+CGG - chr4_2:71170837-71170856 None:intergenic 45.0%
GAGAATTCCGGTATTCTCCA+TGG - chr4_2:71170960-71170979 None:intergenic 45.0%
TGTGAGAACTCTGCTGTTCT+AGG + chr4_2:71172152-71172171 Msa0619840:CDS 45.0%
! TGGCAGGTTGAAAACCAAGA+AGG + chr4_2:71172649-71172668 Msa0619840:intron 45.0%
! GGCAGGTTGAAAACCAAGAA+GGG + chr4_2:71172650-71172669 Msa0619840:intron 45.0%
! GCAGGTTGAAAACCAAGAAG+GGG + chr4_2:71172651-71172670 Msa0619840:intron 45.0%
CAAAGTTTGCAACCCCTTCT+TGG - chr4_2:71172666-71172685 None:intergenic 45.0%
AACATCAGTAGCTTCAGCTC+TGG - chr4_2:71172869-71172888 None:intergenic 45.0%
TGATGTTGCGAATGCAGTTC+TGG + chr4_2:71172882-71172901 Msa0619840:CDS 45.0%
ACAGACTGTGTCATGCTTAG+TGG + chr4_2:71172910-71172929 Msa0619840:CDS 45.0%
GAAGAAGCTAGACTCTCCAA+TGG - chr4_2:71173069-71173088 None:intergenic 45.0%
TGCTAGAGCAGCTCTCATAT+TGG + chr4_2:71173110-71173129 Msa0619840:CDS 45.0%
AGGAGGGACTACTGCAAAAT+TGG + chr4_2:71173143-71173162 Msa0619840:CDS 45.0%
AGGGACTACTGCAAAATTGG+TGG + chr4_2:71173146-71173165 Msa0619840:CDS 45.0%
! AAGGTATCAGTCGTTAGCTC+AGG - chr4_2:71173213-71173232 None:intergenic 45.0%
ATTTGCCCTTCAATGTGCCA+AGG + chr4_2:71173356-71173375 Msa0619840:CDS 45.0%
TTTGCCCTTCAATGTGCCAA+GGG + chr4_2:71173357-71173376 Msa0619840:CDS 45.0%
CTTTCCCTTGGCACATTGAA+GGG - chr4_2:71173364-71173383 None:intergenic 45.0%
CTTCAATGTGCCAAGGGAAA+GGG + chr4_2:71173363-71173382 Msa0619840:CDS 45.0%
CATCCAACGTAGACTGTAGA+TGG - chr4_2:71173550-71173569 None:intergenic 45.0%
!!! ATTTTTTATTATTTTTTTCA+TGG + chr4_2:71172393-71172412 Msa0619840:intron 5.0%
GATCGATGAGATGAGTGGTG+TGG - chr4_2:71170751-71170770 None:intergenic 50.0%
CGCATCTAGGTCTATTCCCA+TGG + chr4_2:71170835-71170854 Msa0619840:CDS 50.0%
CGCAAAAGCTTCTCGACCAT+GGG - chr4_2:71170854-71170873 None:intergenic 50.0%
TCGCAAAAGCTTCTCGACCA+TGG - chr4_2:71170855-71170874 None:intergenic 50.0%
AGAGCTGCCATGGAGAATAC+CGG + chr4_2:71170950-71170969 Msa0619840:CDS 50.0%
TTGGCTCATGATGTGAAGCC+TGG + chr4_2:71172047-71172066 Msa0619840:CDS 50.0%
TGGCTCATGATGTGAAGCCT+GGG + chr4_2:71172048-71172067 Msa0619840:CDS 50.0%
AGTGTCATACTCTGCGCAGA+TGG + chr4_2:71172071-71172090 Msa0619840:CDS 50.0%
GAACAGCAGAGTTCTCACAG+CGG - chr4_2:71172151-71172170 None:intergenic 50.0%
TAATCTGCCTGGAGTCGTAG+TGG + chr4_2:71172190-71172209 Msa0619840:CDS 50.0%
GATGCATTTATGGTGGCACG+TGG + chr4_2:71172706-71172725 Msa0619840:CDS 50.0%
!! CAGTAACGACCGGTTTTCCT+TGG - chr4_2:71172804-71172823 None:intergenic 50.0%
ATCTGAGTCGCAGTAACGAC+CGG - chr4_2:71172814-71172833 None:intergenic 50.0%
CGTTACTGCGACTCAGATGT+TGG + chr4_2:71172816-71172835 Msa0619840:CDS 50.0%
! TCAGTAGCTTCAGCTCTGGT+TGG - chr4_2:71172865-71172884 None:intergenic 50.0%
!! GTTGCGAATGCAGTTCTGGA+TGG + chr4_2:71172886-71172905 Msa0619840:CDS 50.0%
CTTAGTGGTGAAACTGCTGC+TGG + chr4_2:71172925-71172944 Msa0619840:CDS 50.0%
!! GCTGAGAGCACCATTGACTA+TGG + chr4_2:71172997-71173016 Msa0619840:CDS 50.0%
! AGACTCTCCAATGGGCTCAT+GGG - chr4_2:71173060-71173079 None:intergenic 50.0%
! TAGACTCTCCAATGGGCTCA+TGG - chr4_2:71173061-71173080 None:intergenic 50.0%
! TTGGTGGCTAAATACAGGCC+AGG + chr4_2:71173162-71173181 Msa0619840:CDS 50.0%
ACAGAAAGAATTGGCGTGCC+TGG - chr4_2:71173183-71173202 None:intergenic 50.0%
CCTTTCCCTTGGCACATTGA+AGG - chr4_2:71173365-71173384 None:intergenic 50.0%
CCTTCAATGTGCCAAGGGAA+AGG + chr4_2:71173362-71173381 Msa0619840:CDS 50.0%
GGAAAGGGACTCTGCGTTAA+CGG + chr4_2:71173378-71173397 Msa0619840:CDS 50.0%
GAAAGGGACTCTGCGTTAAC+GGG + chr4_2:71173379-71173398 Msa0619840:CDS 50.0%
AAAGGGACTCTGCGTTAACG+GGG + chr4_2:71173380-71173399 Msa0619840:CDS 50.0%
CGTTAACGGGGATTCTGTTG+TGG + chr4_2:71173392-71173411 Msa0619840:CDS 50.0%
GAGTGGTGTGGTGGTTCTTC+TGG - chr4_2:71170739-71170758 None:intergenic 55.0%
CGATGAGATGAGTGGTGTGG+TGG - chr4_2:71170748-71170767 None:intergenic 55.0%
! GTCGAGAAGCTTTTGCGAGC+AGG + chr4_2:71170857-71170876 Msa0619840:CDS 55.0%
CATGACGGCGCAGAGAATTC+CGG - chr4_2:71170972-71170991 None:intergenic 55.0%
CGCCGTCATGCTCGATACTA+AGG + chr4_2:71170982-71171001 Msa0619840:CDS 55.0%
TAGGGGCCTGAGATTCGAAC+TGG + chr4_2:71171912-71171931 Msa0619840:intron 55.0%
GGAAGATCCACTACGACTCC+AGG - chr4_2:71172200-71172219 None:intergenic 55.0%
! TAGCTTCAGCTCTGGTTGGC+CGG - chr4_2:71172861-71172880 None:intergenic 55.0%
ACCAGTACCCATGAGCCCAT+TGG + chr4_2:71173050-71173069 Msa0619840:CDS 55.0%
! TCCAATGGGCTCATGGGTAC+TGG - chr4_2:71173054-71173073 None:intergenic 55.0%
GAGAGCTGCTCTAGCAGAGT+TGG - chr4_2:71173106-71173125 None:intergenic 55.0%
AACGCAGAGTCCCTTTCCCT+TGG - chr4_2:71173376-71173395 None:intergenic 55.0%
! TGTTGTGGTTCTGCATCGCG+TGG + chr4_2:71173407-71173426 Msa0619840:CDS 55.0%
! GTTGTGGTTCTGCATCGCGT+GGG + chr4_2:71173408-71173427 Msa0619840:CDS 55.0%
GCGCAGAGTATGACACTCCC+AGG - chr4_2:71172068-71172087 None:intergenic 60.0%
ATGGTGGCACGTGGTGACCT+TGG + chr4_2:71172715-71172734 Msa0619840:CDS 60.0%
GGCACGTGGTGACCTTGGAA+TGG + chr4_2:71172720-71172739 Msa0619840:CDS 60.0%
! AGCTTCAGCTCTGGTTGGCC+GGG - chr4_2:71172860-71172879 None:intergenic 60.0%
! GCTTCAGCTCTGGTTGGCCG+GGG - chr4_2:71172859-71172878 None:intergenic 65.0%
GCACGCTGGGACCCGCATCT+AGG + chr4_2:71170822-71170841 Msa0619840:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 71170616 71173810 71170616 ID=Msa0619840;Name=Msa0619840
chr4_2 mRNA 71170616 71173810 71170616 ID=Msa0619840-mRNA-1;Parent=Msa0619840;Name=Msa0619840-mRNA-1;_AED=0.23;_eAED=0.23;_QI=160|1|1|1|1|1|3|343|500
chr4_2 exon 71170616 71171003 71170616 ID=Msa0619840-mRNA-1:exon:20941;Parent=Msa0619840-mRNA-1
chr4_2 exon 71171915 71172376 71171915 ID=Msa0619840-mRNA-1:exon:20942;Parent=Msa0619840-mRNA-1
chr4_2 exon 71172655 71173810 71172655 ID=Msa0619840-mRNA-1:exon:20943;Parent=Msa0619840-mRNA-1
chr4_2 five_prime_UTR 71170616 71170775 71170616 ID=Msa0619840-mRNA-1:five_prime_utr;Parent=Msa0619840-mRNA-1
chr4_2 CDS 71170776 71171003 71170776 ID=Msa0619840-mRNA-1:cds;Parent=Msa0619840-mRNA-1
chr4_2 CDS 71171915 71172376 71171915 ID=Msa0619840-mRNA-1:cds;Parent=Msa0619840-mRNA-1
chr4_2 CDS 71172655 71173467 71172655 ID=Msa0619840-mRNA-1:cds;Parent=Msa0619840-mRNA-1
chr4_2 three_prime_UTR 71173468 71173810 71173468 ID=Msa0619840-mRNA-1:three_prime_utr;Parent=Msa0619840-mRNA-1
Gene Sequence

>Msa0619840

ATGGCGACGATTGTAGCAGAGAAGAAGCCCAAAACTAAGATCGTTTGCACGCTGGGACCCGCATCTAGGTCTATTCCCATGGTCGAGAAGCTTTTGCGAGCAGGTATGAACGTTGCTAGATTCAACTTCTCTCATGGTTCTCATGAGTATCATCAGGAAACTCTTGATAATCTTAGAGCTGCCATGGAGAATACCGGAATTCTCTGCGCCGTCATGCTCGATACTAAGGGGCCTGAGATTCGAACTGGATTTCTCAAGGACGCCAAACCTATCCAACTGAAACAAGGTAATGAAATAACTATTTCAACTGACTATAGCCTAAAGGGTGATGAGAATACAATCTCCATGAGCTACAAAAAGTTGGCTCATGATGTGAAGCCTGGGAGTGTCATACTCTGCGCAGATGGAACCATATCATTTACAGTCTTATCGTGTGACAAAGAATTGGGTTTGGTTCAAGTCCGCTGTGAGAACTCTGCTGTTCTAGGCGAGAGAAAGAATGTTAATCTGCCTGGAGTCGTAGTGGATCTTCCCACTTTGACTGAGAAAGACAAGGAAGATATCATGGCTTGGGGAGTTCCCAATAAAATTGACATGATTGCGCTTTCATTTGTTCGAAAAGGTTCTGATCTGGTACAAGTTCGAAAACTTCTAGGACACCATGCTAAGAACATTCTTCTCATGTCAAAGGTTGAAAACCAAGAAGGGGTTGCAAACTTTGATGATATCCTTGCAAACTCTGATGCATTTATGGTGGCACGTGGTGACCTTGGAATGGAAATTCCAATTGAGAAAATATTTCTCGCACAGAAAGTGATGATTTATAAGTGCAATATCCAAGGAAAACCGGTCGTTACTGCGACTCAGATGTTGGAGTCAATGATCAAATCTCCCCGGCCAACCAGAGCTGAAGCTACTGATGTTGCGAATGCAGTTCTGGATGGAACAGACTGTGTCATGCTTAGTGGTGAAACTGCTGCTGGAGCTTATCCAGAACTTGCTGTTCGTACAATGGCTAAAATATGTGTTGAAGCTGAGAGCACCATTGACTATGGAGATGTATTCAAAAGGATAATGGAGCACTCACCAGTACCCATGAGCCCATTGGAGAGTCTAGCTTCTTCTGCTGTTAGAACTGCCAACTCTGCTAGAGCAGCTCTCATATTGGTTCTAACTAGAGGAGGGACTACTGCAAAATTGGTGGCTAAATACAGGCCAGGCACGCCAATTCTTTCTGTTGTCGTTCCTGAGCTAACGACTGATACCTTTGATTGGTCATGCAGTGATGAGTCCCCTGCTAGACATAGCTTGATATTCCGAGGATTGATTCCAATATTAAGTGCTGCTTCTGCTAGAGCTTCTCATGCAGAAACAACAGAAGATGCAATAGAATTTGCCCTTCAATGTGCCAAGGGAAAGGGACTCTGCGTTAACGGGGATTCTGTTGTGGTTCTGCATCGCGTGGGTACCGCATCAATCATCAAAATCTTGACCGTGAAATGA
Protein sequence

>Msa0619840

MATIVAEKKPKTKIVCTLGPASRSIPMVEKLLRAGMNVARFNFSHGSHEYHQETLDNLRAAMENTGILCAVMLDTKGPEIRTGFLKDAKPIQLKQGNEITISTDYSLKGDENTISMSYKKLAHDVKPGSVILCADGTISFTVLSCDKELGLVQVRCENSAVLGERKNVNLPGVVVDLPTLTEKDKEDIMAWGVPNKIDMIALSFVRKGSDLVQVRKLLGHHAKNILLMSKVENQEGVANFDDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTIDYGDVFKRIMEHSPVPMSPLESLASSAVRTANSARAALILVLTRGGTTAKLVAKYRPGTPILSVVVPELTTDTFDWSCSDESPARHSLIFRGLIPILSAASARASHAETTEDAIEFALQCAKGKGLCVNGDSVVVLHRVGTASIIKILTVK*