AlfalfaGEDB | Msa0231370

M. sativa cultivar zhongmu-4 / Msa0231370

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0231370	RYR35552.1	59.783	92	34	1	177	265	94	185	1.14e-19	98.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0231370	A0A445BA41	59.783	92	34	1	177	265	94	185	5.43e-20	98.6

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003890	Msa0231370	0.800091	1.695336e-48	-8.615850e-47
Msa0006110	Msa0231370	0.801783	7.650753e-49	-8.615850e-47
Msa0015610	Msa0231370	0.801744	7.793241e-49	-8.615850e-47
Msa0034110	Msa0231370	0.839096	1.944194e-57	-8.615850e-47
Msa0036140	Msa0231370	0.823506	1.335302e-53	-8.615850e-47
Msa0045790	Msa0231370	0.811306	7.479808e-51	-8.615850e-47
Msa0046160	Msa0231370	0.817428	3.314968e-52	-8.615850e-47
Msa0064800	Msa0231370	0.861188	1.222954e-63	-8.615850e-47
Msa0080090	Msa0231370	0.817189	3.752581e-52	-8.615850e-47
Msa0091460	Msa0231370	0.810626	1.049994e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 273 sgRNAs with CRISPR-Local

Find 688 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCCAAACGAAAAGCAG+GGG	0.622726	2_2:+3588159	None:intergenic
AAACAATCCAGTCTGTAAGC+CGG	0.564048	2_2:+3586718	None:intergenic
AAACTGCTATTGAGAAACAA+AGG	0.604603	2_2:-3588978	Msa0231370:three_prime_UTR
AAACTTGAAATAGAGGAAAA+AGG	0.305445	2_2:+3591033	None:intergenic
AAAGGATGCTTGAGGAAAAC+AGG	0.423850	2_2:-3585212	Msa0231370:three_prime_UTR
AAAGGGTCCAGGCATCAAGC+CGG	0.598580	2_2:-3586737	Msa0231370:three_prime_UTR
AAATCATTAGTTGGCTCTTT+TGG	0.244228	2_2:-3588861	Msa0231370:three_prime_UTR
AAATCTTCCAGTTCAAGCCT+CGG	0.525335	2_2:-3589015	Msa0231370:CDS
AACGAAAAGCAGGGGAGAAT+AGG	0.483486	2_2:+3588167	None:intergenic
AAGAAAGGAAATGGTTATAA+AGG	0.322988	2_2:+3588681	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GACAAGAAAAATCAAATGCA+AGG	-	chr2_2:3584776-3584795	Msa0231370:three_prime_UTR	30.0%
AGAAAAATCAAATGCAAGGA+AGG	-	chr2_2:3584780-3584799	Msa0231370:three_prime_UTR	30.0%
GAAAAATCAAATGCAAGGAA+GGG	-	chr2_2:3584781-3584800	Msa0231370:three_prime_UTR	30.0%
CTGGAAGTTATTTGATTGTT+TGG	+	chr2_2:3585110-3585129	None:intergenic	30.0%
ACTAGCCAAATGGAAATATA+TGG	-	chr2_2:3585159-3585178	Msa0231370:three_prime_UTR	30.0%
TATGGCCATATATTTCCATT+TGG	+	chr2_2:3585167-3585186	None:intergenic	30.0%
TGCTTTCTTCAAAATGGTTA+TGG	+	chr2_2:3585185-3585204	None:intergenic	30.0%
AGTTTATGGAAGAACTTTGT+TGG	+	chr2_2:3585259-3585278	None:intergenic	30.0%
ACAAAGTTCTTCCATAAACT+TGG	-	chr2_2:3585259-3585278	Msa0231370:intron	30.0%
TTGGGAATTATCCAAGTTTA+TGG	+	chr2_2:3585273-3585292	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	3584754	3595566	3584754	ID=Msa0231370;Name=Msa0231370
chr2_2	mRNA	3584754	3595566	3584754	ID=Msa0231370-mRNA-1;Parent=Msa0231370;Name=Msa0231370-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0\|0.8\|0.66\|0.83\|0.8\|0.66\|6\|3712\|273
chr2_2	exon	3584754	3585265	3584754	ID=Msa0231370-mRNA-1:exon:1475;Parent=Msa0231370-mRNA-1
chr2_2	exon	3585787	3586877	3585787	ID=Msa0231370-mRNA-1:exon:1474;Parent=Msa0231370-mRNA-1
chr2_2	exon	3586898	3589173	3586898	ID=Msa0231370-mRNA-1:exon:1473;Parent=Msa0231370-mRNA-1
chr2_2	exon	3590601	3590797	3590601	ID=Msa0231370-mRNA-1:exon:1472;Parent=Msa0231370-mRNA-1
chr2_2	exon	3591018	3591145	3591018	ID=Msa0231370-mRNA-1:exon:1471;Parent=Msa0231370-mRNA-1
chr2_2	exon	3595237	3595566	3595237	ID=Msa0231370-mRNA-1:exon:1470;Parent=Msa0231370-mRNA-1
chr2_2	CDS	3595237	3595566	3595237	ID=Msa0231370-mRNA-1:cds;Parent=Msa0231370-mRNA-1
chr2_2	CDS	3591018	3591145	3591018	ID=Msa0231370-mRNA-1:cds;Parent=Msa0231370-mRNA-1
chr2_2	CDS	3590601	3590797	3590601	ID=Msa0231370-mRNA-1:cds;Parent=Msa0231370-mRNA-1
chr2_2	CDS	3589007	3589173	3589007	ID=Msa0231370-mRNA-1:cds;Parent=Msa0231370-mRNA-1
chr2_2	three_prime_UTR	3586898	3589006	3586898	ID=Msa0231370-mRNA-1:three_prime_utr;Parent=Msa0231370-mRNA-1
chr2_2	three_prime_UTR	3585787	3586877	3585787	ID=Msa0231370-mRNA-1:three_prime_utr;Parent=Msa0231370-mRNA-1
chr2_2	three_prime_UTR	3584754	3585265	3584754	ID=Msa0231370-mRNA-1:three_prime_utr;Parent=Msa0231370-mRNA-1

Gene Sequence

>Msa0231370

ATGCCCTCCGATGCCGAGCCGAGACAAGAAAAATCAAATGCAAGGAAGGGCCATAGTCTACAATGGAAGGAGGTCATATACAAGACCAAGGTTCCAAAGATAAAAGTGATGCCCTCCTATGCCAAGCCGAGACAAGAAAAAACCAAATGCAAGCCAATAGAGATGCATAACCATGTGATGTGTCTTCAAACCATAGAAAACAAAGCCATGTTGATGCATTCAAACCATAGGCCAAACCAATGCTTCCAAGCAATCCAAGGACAGCATGCTAGAGCTCAACAAAGAAACCAACATGTTGAAAACCAATGGCTTCAACAAAATAGCAAACAAGGTTTGCAGATTTTCAATTTCCCAATTCCTCCATACTTTCATCTCGATAACCATAAATTCAACTCTATATCACAACACAACATCTCTCTTCCTTTTTCCTCTATTTCAAGTTTCCCTTCATTTTTCAGGGAAACTTTATCTTTGTCTGATTGTGAAAAGTATATTTTTGAGCTCATTGGGGATCGGGTGTTGGGAGGATGCCTAGAGACTTGTCGCGATCCCGATCGCCTCCCTACAGGCGTAGGCATTCACCGTCACCGGTTGGACATAGATATAGCAGGAGGAGCAGACGTGATAGAAGCCGGTCTCCATATTCGTATAGCAGGCGAAAAAGTCGTTCTATTTCACCAAGAGGCCGCAGAAGTCCTTCTACAACTACTTGGCGTCGCAGAAGTCGATCTCCAACTGCAAAACGATACAGAAGACAAAGAAGTAGGAGTTCCTCATTGTCTCCTGCTCATAAATCTTCCAGTTCAAGCCTCGGGTCAGTAG

Protein sequence

>Msa0231370

MPSDAEPRQEKSNARKGHSLQWKEVIYKTKVPKIKVMPSYAKPRQEKTKCKPIEMHNHVMCLQTIENKAMLMHSNHRPNQCFQAIQGQHARAQQRNQHVENQWLQQNSKQGLQIFNFPIPPYFHLDNHKFNSISQHNISLPFSSISSFPSFFRETLSLSDCEKYIFELIGDRVLGGCLETCRDPDRLPTGVGIHRHRLDIDIAGGADVIEAGLHIRIAGEKVVLFHQEAAEVLLQLLGVAEVDLQLQNDTEDKEVGVPHCLLLINLPVQASGQ*