AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0658630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0658630 MtrunA17_Chr8g0381861 85.714 154 10 1 1 142 137 290 2.59e-84 248
Msa0658630 MtrunA17_Chr3g0123831 53.285 137 42 3 2 123 135 264 2.47e-36 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0658630 AT5G65250.1 58.537 123 38 2 1 111 150 271 2.25e-37 129

Find 69 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCATTTACTTTCATTTC+AGG 0.176402 4_3:+68842799 Msa0658630:three_prime_UTR
GTCTAGGCAGCTTGAGAAAT+TGG 0.177372 4_3:+68839981 Msa0658630:CDS
CTGGTTCTGTGGTAAATTTC+GGG 0.222924 4_3:+68842752 Msa0658630:three_prime_UTR
TTTAACCCAAGCTTCAAATT+TGG 0.231116 4_3:-68842498 None:intergenic
AGCCCTGCAATCAGACATTT+TGG 0.237492 4_3:+68841874 Msa0658630:CDS
CCGTGTTCTTCACTAGTTTC+AGG 0.242419 4_3:-68842418 None:intergenic
TTTATGTGCCAATAAAATTC+TGG 0.256690 4_3:-68842624 None:intergenic
TCTGGTTCTGTGGTAAATTT+CGG 0.258448 4_3:+68842751 Msa0658630:three_prime_UTR
TTTGCAGAGAATTGAGAAAT+TGG 0.262448 4_3:+68839918 Msa0658630:CDS
GGCTAATGCTCTTGCGGCCT+TGG 0.272603 4_3:-68841855 None:intergenic
GAACGGTGGGGTTTCTGTTT+CGG 0.293881 4_3:+68839885 Msa0658630:CDS
TTCATCCCTCTGTAATTTGA+TGG 0.314171 4_3:-68842531 None:intergenic
AGAGTAATGCGACGGTTGTT+AGG 0.336551 4_3:+68839953 Msa0658630:CDS
AGCTTGAGAAATTGGGGATT+AGG 0.351998 4_3:+68839989 Msa0658630:CDS
ACTTTGACTTTGTGTGTAGA+TGG 0.353715 4_3:-68842599 None:intergenic
TAGATTAGTAACTGAGCTAT+TGG 0.353976 4_3:+68842941 Msa0658630:three_prime_UTR
TCTAGGCAGCTTGAGAAATT+GGG 0.354881 4_3:+68839982 Msa0658630:CDS
TCTCCAAAATGTCTGATTGC+AGG 0.356336 4_3:-68841877 None:intergenic
AGATTAGTAACTGAGCTATT+GGG 0.356741 4_3:+68842942 Msa0658630:three_prime_UTR
GTCCACCAAATTTGAAGCTT+GGG 0.368340 4_3:+68842493 Msa0658630:exon
TTGAAGCTTGGGTTAAACTA+AGG 0.401346 4_3:+68842504 Msa0658630:exon
TGGGGTTTCTGTTTCGGAGG+TGG 0.403058 4_3:+68839891 Msa0658630:CDS
ATGAGATAGGATTGAATTCA+TGG 0.407376 4_3:+68842981 Msa0658630:three_prime_UTR
TTGAATGAAGACTGCAGCTC+TGG 0.409148 4_3:+68841820 Msa0658630:intron
TAATGATTACACATTTACTA+AGG 0.435911 4_3:+68842647 Msa0658630:three_prime_UTR
GGTCCACCAAATTTGAAGCT+TGG 0.435933 4_3:+68842492 Msa0658630:exon
GAATCTCAATGCGGTAAATC+AGG 0.435977 4_3:+68842468 Msa0658630:CDS
GAGTAATGCGACGGTTGTTA+GGG 0.448272 4_3:+68839954 Msa0658630:CDS
AACCCAAGCTTCAAATTTGG+TGG 0.451264 4_3:-68842495 None:intergenic
GCAGAGAATTGAGAAATTGG+AGG 0.472739 4_3:+68839921 Msa0658630:CDS
TTGCGAAGGCAGGTAAGCTA+TGG 0.479688 4_3:+68842386 Msa0658630:CDS
CGGTGATTAGGAGAAGGAAA+TGG 0.490410 4_3:+68839848 None:intergenic
ATTCTTGCAATTGCGAAGGC+AGG 0.502036 4_3:+68842376 Msa0658630:CDS
TAGGATTGAATTCATGGTGG+TGG 0.502520 4_3:+68842987 Msa0658630:three_prime_UTR
GGAGAATTGTCTGAACTCTC+TGG 0.503072 4_3:+68842733 Msa0658630:three_prime_UTR
GAGAATTGAGAAATTGGAGG+AGG 0.507588 4_3:+68839924 Msa0658630:CDS
CTCCAAAATGTCTGATTGCA+GGG 0.525218 4_3:-68841876 None:intergenic
ACTTTCATTTCAGGATTACA+AGG 0.527608 4_3:+68842808 Msa0658630:three_prime_UTR
TTACACAACGAGTCAATTCA+AGG 0.531777 4_3:+68842707 Msa0658630:three_prime_UTR
CTAGGCAGCTTGAGAAATTG+GGG 0.538326 4_3:+68839983 Msa0658630:CDS
CGGTGGGGTTTCTGTTTCGG+AGG 0.539820 4_3:+68839888 Msa0658630:CDS
AAGTTTCCCGTGGATACAGA+TGG 0.540326 4_3:+68842677 Msa0658630:three_prime_UTR
GAGAGACGGTGTTGAACGGT+GGG 0.546933 4_3:+68839872 Msa0658630:CDS
GATTAGTAACTGAGCTATTG+GGG 0.561521 4_3:+68842943 Msa0658630:three_prime_UTR
GAGAAGGAAATGGAGAGAGA+CGG 0.562581 4_3:+68839858 None:intergenic
GGAAAACCATCAAATTACAG+AGG 0.566530 4_3:+68842525 Msa0658630:three_prime_UTR
AGTTTCCCGTGGATACAGAT+GGG 0.579307 4_3:+68842678 Msa0658630:three_prime_UTR
GAAAACCATCAAATTACAGA+GGG 0.582046 4_3:+68842526 Msa0658630:three_prime_UTR
AATGATTACACATTTACTAA+GGG 0.583298 4_3:+68842648 Msa0658630:three_prime_UTR
TGGAGAGAGACGGTGTTGAA+CGG 0.589136 4_3:+68839868 Msa0658630:CDS
TGCGAAGGCAGGTAAGCTAT+GGG 0.592521 4_3:+68842387 Msa0658630:CDS
GTCTGAACTCTCTGGTTCTG+TGG 0.592692 4_3:+68842741 Msa0658630:three_prime_UTR
CTGTATCCACGGGAAACTTG+AGG 0.615624 4_3:-68842673 None:intergenic
TTGCAGGGCTAATGCTCTTG+CGG 0.622054 4_3:-68841861 None:intergenic
ACAACGAGTCAATTCAAGGA+GGG 0.627644 4_3:+68842711 Msa0658630:three_prime_UTR
CAACGAGTCAATTCAAGGAG+GGG 0.628381 4_3:+68842712 Msa0658630:three_prime_UTR
GGATTTGAAGAGTAATGCGA+CGG 0.629644 4_3:+68839945 Msa0658630:CDS
TCTCATTCTTGCAATTGCGA+AGG 0.648334 4_3:+68842372 Msa0658630:CDS
AGAGAGACGGTGTTGAACGG+TGG 0.648405 4_3:+68839871 Msa0658630:CDS
AGGGAGCCTCAAGTTTCCCG+TGG 0.653464 4_3:+68842667 Msa0658630:three_prime_UTR
TCTGGCACAGAAGAATTCCA+AGG 0.669972 4_3:+68841838 Msa0658630:CDS
GTTTCCCGTGGATACAGATG+GGG 0.675443 4_3:+68842679 Msa0658630:three_prime_UTR
TCTCAATGCGGTAAATCAGG+AGG 0.687985 4_3:+68842471 Msa0658630:CDS
AGAGACGGTGTTGAACGGTG+GGG 0.693868 4_3:+68839873 Msa0658630:CDS
CCTGAAACTAGTGAAGAACA+CGG 0.696745 4_3:+68842418 Msa0658630:CDS
CAGATGAATATGCATTGGTG+TGG 0.711307 4_3:+68842888 Msa0658630:three_prime_UTR
CACAACGAGTCAATTCAAGG+AGG 0.714306 4_3:+68842710 Msa0658630:three_prime_UTR
CTCAGCTGCGAATCTCAATG+CGG 0.734213 4_3:+68842459 Msa0658630:CDS
AGATAGGATTGAATTCATGG+TGG 0.761795 4_3:+68842984 Msa0658630:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCTTTTTTTTTTTTTTTG+AGG + chr4_3:68840521-68840540 Msa0658630:intron 10.0%
!!! TTCTTTTTTTTTTTTTTTGA+GGG + chr4_3:68840522-68840541 Msa0658630:intron 10.0%
!! ACATTTCATAATAAACTTTA+TGG - chr4_3:68840129-68840148 None:intergenic 15.0%
!! TATTCTATCTAATATTCAAT+AGG - chr4_3:68840370-68840389 None:intergenic 15.0%
!! TATTGAATATTAGATAGAAT+AGG + chr4_3:68840369-68840388 Msa0658630:intron 15.0%
!! TATAAGCAATAATATCATAA+TGG + chr4_3:68840630-68840649 Msa0658630:intron 15.0%
!!! TTTAGTATAGTTTTTACCAA+TGG + chr4_3:68840338-68840357 Msa0658630:intron 20.0%
!!! ATTTTGTTATTTGAACAAGT+CGG - chr4_3:68840440-68840459 None:intergenic 20.0%
!!! AGTTTGTTATTGAACTTTAA+AGG + chr4_3:68842010-68842029 Msa0658630:intron 20.0%
!!! TTAAAGGTAACATTATTTTG+TGG + chr4_3:68842026-68842045 Msa0658630:intron 20.0%
!! TAATGATTACACATTTACTA+AGG + chr4_3:68842647-68842666 Msa0658630:three_prime_UTR 20.0%
!! AATGATTACACATTTACTAA+GGG + chr4_3:68842648-68842667 Msa0658630:three_prime_UTR 20.0%
! ATTATAAAGACGAAAACTGT+TGG - chr4_3:68840195-68840214 None:intergenic 25.0%
! TCAAATATTCTAGCAATGTT+AGG + chr4_3:68840654-68840673 Msa0658630:intron 25.0%
! TTCCTAAAAACATAAGCTTA+TGG + chr4_3:68840853-68840872 Msa0658630:intron 25.0%
!! ATCCATAAGCTTATGTTTTT+AGG - chr4_3:68840858-68840877 None:intergenic 25.0%
!!! TTACTAGTGTTTTTAGCTTA+AGG - chr4_3:68841124-68841143 None:intergenic 25.0%
!!! TATCTTTGAAACAAAGTATG+AGG + chr4_3:68841389-68841408 Msa0658630:intron 25.0%
! AGAGTTTAAGTAACATTGAA+GGG - chr4_3:68841952-68841971 None:intergenic 25.0%
! TTTATGTGCCAATAAAATTC+TGG - chr4_3:68842627-68842646 None:intergenic 25.0%
! TGTTCATTTACTTTCATTTC+AGG + chr4_3:68842799-68842818 Msa0658630:three_prime_UTR 25.0%
!! TTTTTGTTACAGCAAAACTA+AGG - chr4_3:68842868-68842887 None:intergenic 25.0%
! AAAAACAGATGAATATGCAT+TGG + chr4_3:68842883-68842902 Msa0658630:three_prime_UTR 25.0%
!!! AAGTTTGTTTTGTATGAGAT+AGG + chr4_3:68842968-68842987 Msa0658630:three_prime_UTR 25.0%
TTTGCAGAGAATTGAGAAAT+TGG + chr4_3:68839918-68839937 Msa0658630:CDS 30.0%
GTAGTAATTACCTCAGTTAT+AGG - chr4_3:68840045-68840064 None:intergenic 30.0%
ACTGTTGGAATATATTCAAG+AGG - chr4_3:68840180-68840199 None:intergenic 30.0%
! ATTCAATAGGTCTTTTCCAT+TGG - chr4_3:68840357-68840376 None:intergenic 30.0%
! TTAGGTTGCTGACAAAATTA+AGG + chr4_3:68840672-68840691 Msa0658630:intron 30.0%
TTAAGGTTCATAACCAATCT+TGG + chr4_3:68840689-68840708 Msa0658630:intron 30.0%
! CCAGATTGAGTCAATTTTTA+GGG - chr4_3:68841504-68841523 None:intergenic 30.0%
! ACCAGATTGAGTCAATTTTT+AGG - chr4_3:68841505-68841524 None:intergenic 30.0%
TTCCAGTAATATGAATTCCT+CGG - chr4_3:68841584-68841603 None:intergenic 30.0%
!! TCATTAACATTTTGAGTGTC+TGG + chr4_3:68841720-68841739 Msa0658630:intron 30.0%
CAGAGTTTAAGTAACATTGA+AGG - chr4_3:68841953-68841972 None:intergenic 30.0%
AAACTGGAATACTAGATTCA+TGG - chr4_3:68842209-68842228 None:intergenic 30.0%
TTTAACCCAAGCTTCAAATT+TGG - chr4_3:68842501-68842520 None:intergenic 30.0%
GAAAACCATCAAATTACAGA+GGG + chr4_3:68842526-68842545 Msa0658630:three_prime_UTR 30.0%
! CAAAGTAGCCAGAATTTTAT+TGG + chr4_3:68842616-68842635 Msa0658630:three_prime_UTR 30.0%
ACTTTCATTTCAGGATTACA+AGG + chr4_3:68842808-68842827 Msa0658630:three_prime_UTR 30.0%
TAGATTAGTAACTGAGCTAT+TGG + chr4_3:68842941-68842960 Msa0658630:three_prime_UTR 30.0%
AGATTAGTAACTGAGCTATT+GGG + chr4_3:68842942-68842961 Msa0658630:three_prime_UTR 30.0%
ATGAGATAGGATTGAATTCA+TGG + chr4_3:68842981-68843000 Msa0658630:three_prime_UTR 30.0%
TTTGAAGGAACCTATAACTG+AGG + chr4_3:68840032-68840051 Msa0658630:intron 35.0%
! ATATGTGCTGAGAGTTTGTT+GGG + chr4_3:68840490-68840509 Msa0658630:intron 35.0%
!!! TTTTTTGAGGGAGAGTTTGT+TGG + chr4_3:68840534-68840553 Msa0658630:intron 35.0%
!! TTTTTGAGGGAGAGTTTGTT+GGG + chr4_3:68840535-68840554 Msa0658630:intron 35.0%
GGTTGCAATCTAGGTTATTT+AGG - chr4_3:68840748-68840767 None:intergenic 35.0%
ATTGCAACCAGAAGTAGTTA+GGG + chr4_3:68840759-68840778 Msa0658630:intron 35.0%
TGTATTCATGTGTTCGTCAT+TGG + chr4_3:68840811-68840830 Msa0658630:intron 35.0%
ATCGAAGATTGATTGAAAGC+CGG - chr4_3:68840961-68840980 None:intergenic 35.0%
ACTAACCTCAAACTGCTATA+GGG + chr4_3:68840996-68841015 Msa0658630:intron 35.0%
AATAGAGTCTGGCACTTTAT+TGG + chr4_3:68841019-68841038 Msa0658630:intron 35.0%
GGAAGAAAAAAACAGGAATG+TGG + chr4_3:68841193-68841212 Msa0658630:intron 35.0%
! TTGCAAGAAAATGCAGGTTT+TGG + chr4_3:68841341-68841360 Msa0658630:intron 35.0%
! TGCAAGAAAATGCAGGTTTT+GGG + chr4_3:68841342-68841361 Msa0658630:intron 35.0%
CAAAGTATGAGGTTTCAAGA+AGG + chr4_3:68841400-68841419 Msa0658630:intron 35.0%
AGGTCAGAAAACAATGAAAC+TGG + chr4_3:68841424-68841443 Msa0658630:intron 35.0%
GGTCAGAAAACAATGAAACT+GGG + chr4_3:68841425-68841444 Msa0658630:intron 35.0%
GTCAGAAAACAATGAAACTG+GGG + chr4_3:68841426-68841445 Msa0658630:intron 35.0%
! CCCTAAAAATTGACTCAATC+TGG + chr4_3:68841501-68841520 Msa0658630:intron 35.0%
AAATCTCTAGTCTGACGTAT+GGG - chr4_3:68842235-68842254 None:intergenic 35.0%
AAAATCTCTAGTCTGACGTA+TGG - chr4_3:68842236-68842255 None:intergenic 35.0%
! TCGAGTTTGACTTATCTTGA+TGG + chr4_3:68842307-68842326 Msa0658630:intron 35.0%
TGGTGTTTGATCATTCATGT+AGG + chr4_3:68842327-68842346 Msa0658630:intron 35.0%
TTGAAGCTTGGGTTAAACTA+AGG + chr4_3:68842504-68842523 Msa0658630:exon 35.0%
GGAAAACCATCAAATTACAG+AGG + chr4_3:68842525-68842544 Msa0658630:three_prime_UTR 35.0%
TTCATCCCTCTGTAATTTGA+TGG - chr4_3:68842534-68842553 None:intergenic 35.0%
! ACTTTGACTTTGTGTGTAGA+TGG - chr4_3:68842602-68842621 None:intergenic 35.0%
TTACACAACGAGTCAATTCA+AGG + chr4_3:68842707-68842726 Msa0658630:three_prime_UTR 35.0%
!! TCTGGTTCTGTGGTAAATTT+CGG + chr4_3:68842751-68842770 Msa0658630:three_prime_UTR 35.0%
GATTAGTAACTGAGCTATTG+GGG + chr4_3:68842943-68842962 Msa0658630:three_prime_UTR 35.0%
AGATAGGATTGAATTCATGG+TGG + chr4_3:68842984-68843003 Msa0658630:three_prime_UTR 35.0%
GCAGAGAATTGAGAAATTGG+AGG + chr4_3:68839921-68839940 Msa0658630:CDS 40.0%
GAGAATTGAGAAATTGGAGG+AGG + chr4_3:68839924-68839943 Msa0658630:CDS 40.0%
GGATTTGAAGAGTAATGCGA+CGG + chr4_3:68839945-68839964 Msa0658630:CDS 40.0%
TCTAGGCAGCTTGAGAAATT+GGG + chr4_3:68839982-68840001 Msa0658630:CDS 40.0%
AGCTTGAGAAATTGGGGATT+AGG + chr4_3:68839989-68840008 Msa0658630:CDS 40.0%
GATATGTGCTGAGAGTTTGT+TGG + chr4_3:68840489-68840508 Msa0658630:intron 40.0%
! ACTACTTCTGGTTGCAATCT+AGG - chr4_3:68840757-68840776 None:intergenic 40.0%
GATTGCAACCAGAAGTAGTT+AGG + chr4_3:68840758-68840777 Msa0658630:intron 40.0%
TTGCAACCAGAAGTAGTTAG+GGG + chr4_3:68840760-68840779 Msa0658630:intron 40.0%
CTAATTCCCCTAACTACTTC+TGG - chr4_3:68840769-68840788 None:intergenic 40.0%
CGAACACATGAATACACACT+TGG - chr4_3:68840807-68840826 None:intergenic 40.0%
!! CATATCAGGGGTTAGTTTAG+AGG - chr4_3:68840910-68840929 None:intergenic 40.0%
AACCCCTGATATGAGTAGAT+TGG + chr4_3:68840917-68840936 Msa0658630:intron 40.0%
CTCCAATCTACTCATATCAG+GGG - chr4_3:68840922-68840941 None:intergenic 40.0%
GCTCCAATCTACTCATATCA+GGG - chr4_3:68840923-68840942 None:intergenic 40.0%
AGCTCCAATCTACTCATATC+AGG - chr4_3:68840924-68840943 None:intergenic 40.0%
TCGAAGATTGATTGAAAGCC+GGG - chr4_3:68840960-68840979 None:intergenic 40.0%
GACTAACCTCAAACTGCTAT+AGG + chr4_3:68840995-68841014 Msa0658630:intron 40.0%
CTATTCCCTATAGCAGTTTG+AGG - chr4_3:68841004-68841023 None:intergenic 40.0%
ACAGAAGACTTAGAACACAG+CGG + chr4_3:68841171-68841190 Msa0658630:intron 40.0%
GTGGAATCTGAGCAAAAGAT+AGG + chr4_3:68841212-68841231 Msa0658630:intron 40.0%
ATGAGCTTGCAAGAAAATGC+AGG + chr4_3:68841335-68841354 Msa0658630:intron 40.0%
!!! AAGAAAATGCAGGTTTTGGG+TGG + chr4_3:68841345-68841364 Msa0658630:intron 40.0%
!! GTTTTGGGTGGAAATGACAT+TGG + chr4_3:68841357-68841376 Msa0658630:intron 40.0%
GTATGAGGTTTCAAGAAGGA+AGG + chr4_3:68841404-68841423 Msa0658630:intron 40.0%
! AGTGGCTGCTTTCATGAAAT+GGG - chr4_3:68841467-68841486 None:intergenic 40.0%
! TAGTGGCTGCTTTCATGAAA+TGG - chr4_3:68841468-68841487 None:intergenic 40.0%
! AATTGACTCAATCTGGTTGC+AGG + chr4_3:68841508-68841527 Msa0658630:intron 40.0%
ACAGTACAGGATCTACATCT+CGG - chr4_3:68841553-68841572 None:intergenic 40.0%
CAGTAATATGAATTCCTCGG+AGG - chr4_3:68841581-68841600 None:intergenic 40.0%
CTCCGAGGAATTCATATTAC+TGG + chr4_3:68841579-68841598 Msa0658630:intron 40.0%
!! CATTTTGAGTGTCTGGTTGA+AGG + chr4_3:68841727-68841746 Msa0658630:intron 40.0%
CTCCAAAATGTCTGATTGCA+GGG - chr4_3:68841879-68841898 None:intergenic 40.0%
TCTCCAAAATGTCTGATTGC+AGG - chr4_3:68841880-68841899 None:intergenic 40.0%
!! GCAATCAGACATTTTGGAGA+AGG + chr4_3:68841880-68841899 Msa0658630:CDS 40.0%
! AAAGGTTTTGCTAGCAATGC+AGG + chr4_3:68841919-68841938 Msa0658630:CDS 40.0%
!! AACACTTTAGCTCTCATGTG+CGG + chr4_3:68842066-68842085 Msa0658630:intron 40.0%
CACATCATTGTGTTGTGTGT+GGG - chr4_3:68842149-68842168 None:intergenic 40.0%
TCACATCATTGTGTTGTGTG+TGG - chr4_3:68842150-68842169 None:intergenic 40.0%
GGAATACTAGATTCATGGTC+TGG - chr4_3:68842204-68842223 None:intergenic 40.0%
TCTGACGTATGGGTTTAAAC+TGG - chr4_3:68842225-68842244 None:intergenic 40.0%
TCTCATTCTTGCAATTGCGA+AGG + chr4_3:68842372-68842391 Msa0658630:CDS 40.0%
CCTGAAACTAGTGAAGAACA+CGG + chr4_3:68842418-68842437 Msa0658630:CDS 40.0%
GAATCTCAATGCGGTAAATC+AGG + chr4_3:68842468-68842487 Msa0658630:CDS 40.0%
! GTCCACCAAATTTGAAGCTT+GGG + chr4_3:68842493-68842512 Msa0658630:exon 40.0%
AACCCAAGCTTCAAATTTGG+TGG - chr4_3:68842498-68842517 None:intergenic 40.0%
! TTTTTCCCCATCTGTATCCA+CGG - chr4_3:68842687-68842706 None:intergenic 40.0%
ACAACGAGTCAATTCAAGGA+GGG + chr4_3:68842711-68842730 Msa0658630:three_prime_UTR 40.0%
!! CTGGTTCTGTGGTAAATTTC+GGG + chr4_3:68842752-68842771 Msa0658630:three_prime_UTR 40.0%
! CAGATGAATATGCATTGGTG+TGG + chr4_3:68842888-68842907 Msa0658630:three_prime_UTR 40.0%
TAGGATTGAATTCATGGTGG+TGG + chr4_3:68842987-68843006 Msa0658630:three_prime_UTR 40.0%
AGAGTAATGCGACGGTTGTT+AGG + chr4_3:68839953-68839972 Msa0658630:CDS 45.0%
GAGTAATGCGACGGTTGTTA+GGG + chr4_3:68839954-68839973 Msa0658630:CDS 45.0%
!! CGGTTGTTAGGGTTTTGTCT+AGG + chr4_3:68839965-68839984 Msa0658630:CDS 45.0%
GTCTAGGCAGCTTGAGAAAT+TGG + chr4_3:68839981-68840000 Msa0658630:CDS 45.0%
CTAGGCAGCTTGAGAAATTG+GGG + chr4_3:68839983-68840002 Msa0658630:CDS 45.0%
GGATTAGGTTTCGTGTTACC+AGG + chr4_3:68840004-68840023 Msa0658630:intron 45.0%
TAGGTTTCGTGTTACCAGGA+AGG + chr4_3:68840008-68840027 Msa0658630:intron 45.0%
AGGTTTCGTGTTACCAGGAA+GGG + chr4_3:68840009-68840028 Msa0658630:intron 45.0%
TGTTACCAGGAAGGGTTTGA+AGG + chr4_3:68840017-68840036 Msa0658630:intron 45.0%
GAGCTTGAAATGCTAATGCC+CGG + chr4_3:68840939-68840958 Msa0658630:intron 45.0%
CTGCTATAGGGAATAGAGTC+TGG + chr4_3:68841008-68841027 Msa0658630:intron 45.0%
CAGAAGACTTAGAACACAGC+GGG + chr4_3:68841172-68841191 Msa0658630:intron 45.0%
CACAGCGGGAAGAAAAAAAC+AGG + chr4_3:68841186-68841205 Msa0658630:intron 45.0%
! TGGCTGCTTTCATGAAATGG+GGG - chr4_3:68841465-68841484 None:intergenic 45.0%
! GTGGCTGCTTTCATGAAATG+GGG - chr4_3:68841466-68841485 None:intergenic 45.0%
AGGGAGATTCACAGTTGTAG+TGG - chr4_3:68841485-68841504 None:intergenic 45.0%
TTGAATGAAGACTGCAGCTC+TGG + chr4_3:68841820-68841839 Msa0658630:intron 45.0%
TCTGGCACAGAAGAATTCCA+AGG + chr4_3:68841838-68841857 Msa0658630:CDS 45.0%
! AGCCCTGCAATCAGACATTT+TGG + chr4_3:68841874-68841893 Msa0658630:CDS 45.0%
!! AGACATTTTGGAGAAGGAGC+TGG + chr4_3:68841886-68841905 Msa0658630:CDS 45.0%
!! GACATTTTGGAGAAGGAGCT+GGG + chr4_3:68841887-68841906 Msa0658630:CDS 45.0%
!! ACATTTTGGAGAAGGAGCTG+GGG + chr4_3:68841888-68841907 Msa0658630:CDS 45.0%
ATTGTGTTGTGTGTGGGTGT+GGG - chr4_3:68842143-68842162 None:intergenic 45.0%
ATTCTTGCAATTGCGAAGGC+AGG + chr4_3:68842376-68842395 Msa0658630:CDS 45.0%
! CCGTGTTCTTCACTAGTTTC+AGG - chr4_3:68842421-68842440 None:intergenic 45.0%
TCTCAATGCGGTAAATCAGG+AGG + chr4_3:68842471-68842490 Msa0658630:CDS 45.0%
! GGTCCACCAAATTTGAAGCT+TGG + chr4_3:68842492-68842511 Msa0658630:exon 45.0%
AAGTTTCCCGTGGATACAGA+TGG + chr4_3:68842677-68842696 Msa0658630:three_prime_UTR 45.0%
AGTTTCCCGTGGATACAGAT+GGG + chr4_3:68842678-68842697 Msa0658630:three_prime_UTR 45.0%
! TTTTCCCCATCTGTATCCAC+GGG - chr4_3:68842686-68842705 None:intergenic 45.0%
CACAACGAGTCAATTCAAGG+AGG + chr4_3:68842710-68842729 Msa0658630:three_prime_UTR 45.0%
CAACGAGTCAATTCAAGGAG+GGG + chr4_3:68842712-68842731 Msa0658630:three_prime_UTR 45.0%
GGAGAATTGTCTGAACTCTC+TGG + chr4_3:68842733-68842752 Msa0658630:three_prime_UTR 45.0%
! TGGAGAGAGACGGTGTTGAA+CGG + chr4_3:68839868-68839887 Msa0658630:CDS 50.0%
GAACGGTGGGGTTTCTGTTT+CGG + chr4_3:68839885-68839904 Msa0658630:CDS 50.0%
AGGTTCCTTCAAACCCTTCC+TGG - chr4_3:68840025-68840044 None:intergenic 50.0%
ACCAATCTTGGCAGCTCTAC+AGG + chr4_3:68840701-68840720 Msa0658630:intron 50.0%
TCCTGTAGAGCTGCCAAGAT+TGG - chr4_3:68840705-68840724 None:intergenic 50.0%
TTGCAGGGCTAATGCTCTTG+CGG - chr4_3:68841864-68841883 None:intergenic 50.0%
!! CATTTTGGAGAAGGAGCTGG+GGG + chr4_3:68841889-68841908 Msa0658630:CDS 50.0%
CATTGTGTTGTGTGTGGGTG+TGG - chr4_3:68842144-68842163 None:intergenic 50.0%
TTGCGAAGGCAGGTAAGCTA+TGG + chr4_3:68842386-68842405 Msa0658630:CDS 50.0%
TGCGAAGGCAGGTAAGCTAT+GGG + chr4_3:68842387-68842406 Msa0658630:CDS 50.0%
CTCAGCTGCGAATCTCAATG+CGG + chr4_3:68842459-68842478 Msa0658630:CDS 50.0%
CTGTATCCACGGGAAACTTG+AGG - chr4_3:68842676-68842695 None:intergenic 50.0%
GTTTCCCGTGGATACAGATG+GGG + chr4_3:68842679-68842698 Msa0658630:three_prime_UTR 50.0%
!! GTCTGAACTCTCTGGTTCTG+TGG + chr4_3:68842741-68842760 Msa0658630:three_prime_UTR 50.0%
! AGAGAGACGGTGTTGAACGG+TGG + chr4_3:68839871-68839890 Msa0658630:CDS 55.0%
! GAGAGACGGTGTTGAACGGT+GGG + chr4_3:68839872-68839891 Msa0658630:CDS 55.0%
! AGAGACGGTGTTGAACGGTG+GGG + chr4_3:68839873-68839892 Msa0658630:CDS 55.0%
TGGGGTTTCTGTTTCGGAGG+TGG + chr4_3:68839891-68839910 Msa0658630:CDS 55.0%
CTCGGAGGACACTACAGTAC+AGG - chr4_3:68841566-68841585 None:intergenic 55.0%
CTGTACTGTAGTGTCCTCCG+AGG + chr4_3:68841564-68841583 Msa0658630:intron 55.0%
GGAGCTGGGGGAAGTTCAAA+AGG + chr4_3:68841901-68841920 Msa0658630:CDS 55.0%
CGGTGGGGTTTCTGTTTCGG+AGG + chr4_3:68839888-68839907 Msa0658630:CDS 60.0%
GGCTAATGCTCTTGCGGCCT+TGG - chr4_3:68841858-68841877 None:intergenic 60.0%
AGGGAGCCTCAAGTTTCCCG+TGG + chr4_3:68842667-68842686 Msa0658630:three_prime_UTR 60.0%
GGGTGTGGGTGTGCGTAGGT+GGG - chr4_3:68842129-68842148 None:intergenic 65.0%
TGGGTGTGGGTGTGCGTAGG+TGG - chr4_3:68842130-68842149 None:intergenic 65.0%
GTGTGGGTGTGGGTGTGCGT+AGG - chr4_3:68842133-68842152 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 68839859 68843022 68839859 ID=Msa0658630;Name=Msa0658630
chr4_3 mRNA 68839859 68843022 68839859 ID=Msa0658630-mRNA-1;Parent=Msa0658630;Name=Msa0658630-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0.5|0.66|1|0.5|0.66|3|515|142
chr4_3 exon 68839859 68840017 68839859 ID=Msa0658630-mRNA-1:exon:20015;Parent=Msa0658630-mRNA-1
chr4_3 exon 68841830 68841940 68841830 ID=Msa0658630-mRNA-1:exon:20016;Parent=Msa0658630-mRNA-1
chr4_3 exon 68842349 68843022 68842349 ID=Msa0658630-mRNA-1:exon:20017;Parent=Msa0658630-mRNA-1
chr4_3 CDS 68839859 68840017 68839859 ID=Msa0658630-mRNA-1:cds;Parent=Msa0658630-mRNA-1
chr4_3 CDS 68841830 68841940 68841830 ID=Msa0658630-mRNA-1:cds;Parent=Msa0658630-mRNA-1
chr4_3 CDS 68842349 68842507 68842349 ID=Msa0658630-mRNA-1:cds;Parent=Msa0658630-mRNA-1
chr4_3 three_prime_UTR 68842508 68843022 68842508 ID=Msa0658630-mRNA-1:three_prime_utr;Parent=Msa0658630-mRNA-1
Gene Sequence

>Msa0658630

AGAAGGAAATGGAGAGAGACGGTGTTGAACGGTGGGGTTTCTGTTTCGGAGGTGGAATTTTTGCAGAGAATTGAGAAATTGGAGGAGGATTTGAAGAGTAATGCGACGGTTGTTAGGGTTTTGTCTAGGCAGCTTGAGAAATTGGGGATTAGGTTTCGTACTGCAGCTCTGGCACAGAAGAATTCCAAGGCCGCAAGAGCATTAGCCCTGCAATCAGACATTTTGGAGAAGGAGCTGGGGGAAGTTCAAAAGGTTTTGCTAGCAATGCAGGAACAACAGCAAAAACAACTTGATCTCATTCTTGCAATTGCGAAGGCAGGTAAGCTATGGGAAAGCAAGCCTGAAACTAGTGAAGAACACGGTACAATAGATATGTCTAACTCAGCTGCGAATCTCAATGCGGTAAATCAGGAGGTCCACCAAATTTGA
Protein sequence

>Msa0658630

RRKWRETVLNGGVSVSEVEFLQRIEKLEEDLKSNATVVRVLSRQLEKLGIRFRTAALAQKNSKAARALALQSDILEKELGEVQKVLLAMQEQQQKQLDLILAIAKAGKLWESKPETSEEHGTIDMSNSAANLNAVNQEVHQI*