AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0658900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCACAATTGTAAATAATT+AGG 0.257693 4_3:-69092996 Msa0658900:three_prime_UTR
TGGTAATATTTGTCCATATT+GGG 0.282086 4_3:-69093171 Msa0658900:CDS
TGAAGATGGTCAGTTGTATA+TGG 0.293940 4_3:-69093063 Msa0658900:three_prime_UTR
ATTCTTTAGGCAACCCAATA+TGG 0.309483 4_3:+69093158 None:intergenic
TTGGTAATATTTGTCCATAT+TGG 0.311792 4_3:-69093172 Msa0658900:CDS
TAGGTGTTTCTACAAATTAT+TGG 0.319434 4_3:-69092977 Msa0658900:three_prime_UTR
TGGTTGTGAGGGATAAAATA+AGG 0.335775 4_3:+69096254 None:intergenic
AAACTGAAGTTGGTTTCATT+AGG 0.366193 4_3:-69092931 Msa0658900:three_prime_UTR
AGAGAGAGTGGTGGTAGGTT+TGG 0.386635 4_3:+69096234 None:intergenic
TTATTAATGAAAACTGAAGT+TGG 0.406369 4_3:-69092941 Msa0658900:three_prime_UTR
TGGTGGTAGGTTTGGTTGTG+AGG 0.433839 4_3:+69096242 None:intergenic
GAAAGAAGGTAGAGGAAGAA+TGG 0.435986 4_3:+69096164 None:intergenic
AGTATATGTGAAAACAGTAT+TGG 0.437391 4_3:-69092866 Msa0658900:three_prime_UTR
CGACGTTTCAAAGCATCATC+AGG 0.447392 4_3:-69095988 Msa0658900:intron
CGTGAGAGAGAAAGAGAATG+AGG 0.449011 4_3:+69096209 None:intergenic
GAGAATGAGGGAAGAGAGAG+TGG 0.454177 4_3:+69096222 None:intergenic
GAATGTATAAATGAATTAGT+TGG 0.455497 4_3:+69096064 None:intergenic
GATAACAACCAAAACAAAAC+AGG 0.457405 4_3:-69093109 Msa0658900:three_prime_UTR
ACAGACTCATGAGATTCTTT+AGG 0.457748 4_3:+69093145 None:intergenic
ATATATGTTCACTCAAGGTT+TGG 0.459413 4_3:-69093191 Msa0658900:CDS
CTTATTGAAGGGATAATACT+AGG 0.484084 4_3:-69092810 None:intergenic
GGTGGTAGGTTTGGTTGTGA+GGG 0.485295 4_3:+69096243 None:intergenic
CTGCTAACATGAGCAGCATT+CGG 0.491082 4_3:-69095241 Msa0658900:CDS
AATTCCGACGCGGTGGTCAA+TGG 0.496352 4_3:+69096018 None:intergenic
AGAAGGTAGAGGAAGAATGG+TGG 0.518556 4_3:+69096167 None:intergenic
GGATGAAGAGAGGAAGATGA+TGG 0.529212 4_3:+69096096 None:intergenic
AGAGAATATATGTTCACTCA+AGG 0.533933 4_3:-69093196 Msa0658900:CDS
GTGAGAGAGAAAGAGAATGA+GGG 0.546907 4_3:+69096210 None:intergenic
CGTACAAGAAGTGTGCGTCC+TGG 0.549349 4_3:+69095832 None:intergenic
GCATAATGAAAGAATGAGCC+AGG 0.551370 4_3:-69095850 Msa0658900:exon
AGGGAAGAGAGAGTGGTGGT+AGG 0.554495 4_3:+69096229 None:intergenic
GGTGGTCTCAAAATTGAACT+TGG 0.556174 4_3:+69096039 None:intergenic
CAGGACGCACACTTCTTGTA+CGG 0.559296 4_3:-69095831 Msa0658900:CDS
TGAAGAGAGGAAGATGATGG+TGG 0.578560 4_3:+69096099 None:intergenic
AAAATGAAGAAAGAAGGTAG+AGG 0.585853 4_3:+69096156 None:intergenic
CTGATGATGCTTTGAAACGT+CGG 0.595597 4_3:+69095989 None:intergenic
AATGAGGGAAGAGAGAGTGG+TGG 0.600251 4_3:+69096225 None:intergenic
TCCGACGCGGTGGTCAATGG+TGG 0.610146 4_3:+69096021 None:intergenic
GACGCACACTTCTTGTACGG+AGG 0.632791 4_3:-69095828 Msa0658900:intron
ACCACCATTGACCACCGCGT+CGG 0.649259 4_3:-69096022 Msa0658900:five_prime_UTR
GAGAGCGAATTCCGACGCGG+TGG 0.690172 4_3:+69096011 None:intergenic
TCGGAGAGCGAATTCCGACG+CGG 0.766054 4_3:+69096008 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAATACCAAATAAAAT+TGG + chr4_3:69093328-69093347 None:intergenic 10.0%
!! AATATAAAAGCTATTATAAA+AGG + chr4_3:69093579-69093598 None:intergenic 10.0%
!! ATAAATCTGAAAATGAAAAA+TGG + chr4_3:69093274-69093293 None:intergenic 15.0%
!! ACAAATAAGAAAAGTTAAAA+GGG + chr4_3:69093388-69093407 None:intergenic 15.0%
!! TACAAATAAGAAAAGTTAAA+AGG + chr4_3:69093389-69093408 None:intergenic 15.0%
!!! AATTCAATTATTTTATTTGC+AGG - chr4_3:69093783-69093802 Msa0658900:intron 15.0%
!! GATTTAATAGAAACAATTAA+TGG + chr4_3:69093909-69093928 None:intergenic 15.0%
!!! GATTGTTAATCTATTTTTTT+GGG - chr4_3:69094550-69094569 Msa0658900:intron 15.0%
!! TAATTTATCTTTGCATAATA+AGG - chr4_3:69094649-69094668 Msa0658900:intron 15.0%
!!! TTATTATAATCACATCTTTT+AGG - chr4_3:69094989-69095008 Msa0658900:intron 15.0%
!! TATAAGTTTGAATTTCAAAA+TGG + chr4_3:69095248-69095267 None:intergenic 15.0%
!!! TATTTAGTATTTTCTCTTTT+TGG - chr4_3:69095666-69095685 Msa0658900:intron 15.0%
!!! ATTTAGTATTTTCTCTTTTT+GGG - chr4_3:69095667-69095686 Msa0658900:intron 15.0%
!! AATCACAATTGTAAATAATT+AGG - chr4_3:69096069-69096088 Msa0658900:five_prime_UTR 15.0%
!!! AATTTTGTTAATCTTATTGA+AGG - chr4_3:69096243-69096262 Msa0658900:five_prime_UTR 15.0%
!!! ATTTTGTTAATCTTATTGAA+GGG - chr4_3:69096244-69096263 Msa0658900:five_prime_UTR 15.0%
!!! TCTTCATTTTTGATCAATTT+CGG - chr4_3:69092922-69092941 Msa0658900:three_prime_UTR 20.0%
!! GAATGTATAAATGAATTAGT+TGG + chr4_3:69093004-69093023 None:intergenic 20.0%
!! TCTCAAAATGCAAAATATAA+TGG + chr4_3:69093301-69093320 None:intergenic 20.0%
!! AAATACCAAATAAAATTGGT+TGG + chr4_3:69093324-69093343 None:intergenic 20.0%
!! TAATAGAAACAATTAATGGA+AGG + chr4_3:69093905-69093924 None:intergenic 20.0%
!! ACAAATAATGACAATCAAAT+GGG + chr4_3:69093947-69093966 None:intergenic 20.0%
!! TACAAATAATGACAATCAAA+TGG + chr4_3:69093948-69093967 None:intergenic 20.0%
!!! TGTTATTGATGTTAATTTGA+TGG - chr4_3:69094022-69094041 Msa0658900:intron 20.0%
!! TTTGAATAACAAAATGATAG+TGG + chr4_3:69094161-69094180 None:intergenic 20.0%
!!! GGATTGTTAATCTATTTTTT+TGG - chr4_3:69094549-69094568 Msa0658900:intron 20.0%
!!! TAGCTATAACTTTCTATTTT+TGG - chr4_3:69094584-69094603 Msa0658900:intron 20.0%
!!! TCTATTATTTCTTAGATGTA+TGG - chr4_3:69094687-69094706 Msa0658900:intron 20.0%
!!! AAAATTTAGAGTGTATAGAA+TGG - chr4_3:69094713-69094732 Msa0658900:intron 20.0%
!!! CTACTAATAACTACTATTTT+TGG + chr4_3:69094784-69094803 None:intergenic 20.0%
!!! ATACTTTTGACATAAAATCA+TGG + chr4_3:69095729-69095748 None:intergenic 20.0%
!! TTATTAATGAAAACTGAAGT+TGG - chr4_3:69096124-69096143 Msa0658900:five_prime_UTR 20.0%
! TGATCAAAAATGAAGAAAGA+AGG + chr4_3:69092918-69092937 None:intergenic 25.0%
! ACTATTAACAGATCGAAAAT+GGG + chr4_3:69093135-69093154 None:intergenic 25.0%
! TACTATTAACAGATCGAAAA+TGG + chr4_3:69093136-69093155 None:intergenic 25.0%
!!! GTGTTTGTAGTTTTGAATTT+TGG - chr4_3:69093185-69093204 Msa0658900:CDS 25.0%
! AGAAATATGAGATTAGATAG+AGG + chr4_3:69093508-69093527 None:intergenic 25.0%
! AATCTCATATTTCTTGATTC+TGG - chr4_3:69093514-69093533 Msa0658900:intron 25.0%
! ATTTAATTGAGATGAGTTTG+TGG - chr4_3:69093638-69093657 Msa0658900:intron 25.0%
!!! TCAATTATTTTATTTGCAGG+CGG - chr4_3:69093786-69093805 Msa0658900:intron 25.0%
! AGTTCTTCCAAATTTATTGT+AGG - chr4_3:69093862-69093881 Msa0658900:intron 25.0%
! CAAATAATGACAATCAAATG+GGG + chr4_3:69093946-69093965 None:intergenic 25.0%
!!! TTGAAATTTGAAAGTGTTAG+TGG + chr4_3:69094129-69094148 None:intergenic 25.0%
!!! AATAACAAAATGATAGTGGA+GGG + chr4_3:69094157-69094176 None:intergenic 25.0%
! TCAAACAAATATCCACTAAA+AGG - chr4_3:69094176-69094195 Msa0658900:intron 25.0%
! AACCTCAAAATTAAAGTACA+GGG + chr4_3:69094341-69094360 None:intergenic 25.0%
! AAACCTCAAAATTAAAGTAC+AGG + chr4_3:69094342-69094361 None:intergenic 25.0%
!!! TTTTGAGGTTTAAAGACATA+TGG - chr4_3:69094351-69094370 Msa0658900:intron 25.0%
! AAATAACATGCAAACAATTC+AGG + chr4_3:69094421-69094440 None:intergenic 25.0%
! GTAGTATATTAGATCAATCT+AGG - chr4_3:69094460-69094479 Msa0658900:intron 25.0%
!! GCATAAGCAATTTTCTAAAT+TGG + chr4_3:69094504-69094523 None:intergenic 25.0%
!!! ATCTATTTTTTTGGGTGTTT+TGG - chr4_3:69094558-69094577 Msa0658900:intron 25.0%
! CTTTGCATAATAAGGTAAAT+TGG - chr4_3:69094657-69094676 Msa0658900:intron 25.0%
! AATTATATTCCTCTTGAACA+TGG - chr4_3:69094855-69094874 Msa0658900:intron 25.0%
! TTTGAATTTCAAAATGGTAG+TGG + chr4_3:69095242-69095261 None:intergenic 25.0%
! ATATCACACAGAAAACAAAA+GGG - chr4_3:69095311-69095330 Msa0658900:intron 25.0%
! AAAACAAAAGGGACAATAAT+GGG - chr4_3:69095322-69095341 Msa0658900:intron 25.0%
!!! TAAAAGTATTGGAAAAGACA+TGG + chr4_3:69095358-69095377 None:intergenic 25.0%
! TACTACAAATAGACAAACAT+GGG + chr4_3:69095385-69095404 None:intergenic 25.0%
! ATACTACAAATAGACAAACA+TGG + chr4_3:69095386-69095405 None:intergenic 25.0%
!!! TTTAGATTTACATGTCGTTT+TGG - chr4_3:69095555-69095574 Msa0658900:intron 25.0%
! TTCAAAGAAATCTTTAGCTA+AGG + chr4_3:69095706-69095725 None:intergenic 25.0%
! TCAAGAAGACTTGAATAATT+AGG + chr4_3:69095763-69095782 None:intergenic 25.0%
!! TTGGTAATATTTGTCCATAT+TGG - chr4_3:69095893-69095912 Msa0658900:intron 25.0%
! TGGTAATATTTGTCCATATT+GGG - chr4_3:69095894-69095913 Msa0658900:intron 25.0%
!! TAGGTGTTTCTACAAATTAT+TGG - chr4_3:69096088-69096107 Msa0658900:five_prime_UTR 25.0%
! AGTATATGTGAAAACAGTAT+TGG - chr4_3:69096199-69096218 Msa0658900:five_prime_UTR 25.0%
AAAATGAAGAAAGAAGGTAG+AGG + chr4_3:69092912-69092931 None:intergenic 30.0%
!!! TGAATTAGTTGGTTTTGTAG+AGG + chr4_3:69092993-69093012 None:intergenic 30.0%
! AGATCGAAAATGGGTATTTT+CGG + chr4_3:69093126-69093145 None:intergenic 30.0%
! TGAGACCAACCAATTTTATT+TGG - chr4_3:69093316-69093335 Msa0658900:intron 30.0%
! ACATTGCGATTTTATGCTTT+TGG - chr4_3:69093463-69093482 Msa0658900:intron 30.0%
! CATATTTCTTGATTCTGGAA+TGG - chr4_3:69093519-69093538 Msa0658900:intron 30.0%
CTCAATAGTATCACATAACT+TGG + chr4_3:69093741-69093760 None:intergenic 30.0%
GGAAGAACCTACAATAAATT+TGG + chr4_3:69093872-69093891 None:intergenic 30.0%
TTATTGTAGGTTCTTCCATT+TGG - chr4_3:69093875-69093894 Msa0658900:intron 30.0%
!! GAATAACAAAATGATAGTGG+AGG + chr4_3:69094158-69094177 None:intergenic 30.0%
! TTTGAAATTTGCATGTGTCT+TGG + chr4_3:69094314-69094333 None:intergenic 30.0%
!! TTCCCTGTACTTTAATTTTG+AGG - chr4_3:69094336-69094355 Msa0658900:intron 30.0%
TTCAGGAGAAAATACAGAAA+AGG + chr4_3:69094404-69094423 None:intergenic 30.0%
! TCAGTGCAATTTATTTGTTG+TGG - chr4_3:69094528-69094547 Msa0658900:intron 30.0%
TTTCATTGAGAGAAACAAAG+TGG + chr4_3:69094837-69094856 None:intergenic 30.0%
TGAAGATTCTGTTAAGAAGA+AGG + chr4_3:69094963-69094982 None:intergenic 30.0%
CTCATAAAATGGAAACAACT+TGG + chr4_3:69095064-69095083 None:intergenic 30.0%
CATTCGTTCTTCTCATAAAA+TGG + chr4_3:69095075-69095094 None:intergenic 30.0%
! TTTTATGAGAAGAACGAATG+CGG - chr4_3:69095075-69095094 Msa0658900:intron 30.0%
CATATCACACAGAAAACAAA+AGG - chr4_3:69095310-69095329 Msa0658900:intron 30.0%
GAAAACAAAAGGGACAATAA+TGG - chr4_3:69095321-69095340 Msa0658900:intron 30.0%
! ACATGGGAATGTAAAAGTAT+TGG + chr4_3:69095369-69095388 None:intergenic 30.0%
AGAGAATATATGTTCACTCA+AGG - chr4_3:69095869-69095888 Msa0658900:five_prime_UTR 30.0%
ATATATGTTCACTCAAGGTT+TGG - chr4_3:69095874-69095893 Msa0658900:intron 30.0%
GATAACAACCAAAACAAAAC+AGG - chr4_3:69095956-69095975 Msa0658900:intron 30.0%
!! TGTGATGTTTCTTTTGAAGA+TGG - chr4_3:69095988-69096007 Msa0658900:intron 30.0%
! AAACTGAAGTTGGTTTCATT+AGG - chr4_3:69096134-69096153 Msa0658900:five_prime_UTR 30.0%
! TTGCGATTTTATGCTTTTGG+TGG - chr4_3:69093466-69093485 Msa0658900:intron 35.0%
!! AACACTTGCTATAGTGAACA+TGG - chr4_3:69093673-69093692 Msa0658900:intron 35.0%
TATAGTGAACATGGTACTCA+TGG - chr4_3:69093682-69093701 Msa0658900:intron 35.0%
TTAATGGAAGGAACACCAAA+TGG + chr4_3:69093893-69093912 None:intergenic 35.0%
ACAAATATCCACTAAAAGGC+AGG - chr4_3:69094180-69094199 Msa0658900:intron 35.0%
! GTGCAAGTCAGTTTTCATTA+TGG + chr4_3:69094242-69094261 None:intergenic 35.0%
!! AAGTTAGCTTGCTGTTCTAT+TGG - chr4_3:69094740-69094759 Msa0658900:intron 35.0%
AGAGAAACAAAGTGGCAAAA+TGG + chr4_3:69094829-69094848 None:intergenic 35.0%
AAACTGAAACCATGTTCAAG+AGG + chr4_3:69094867-69094886 None:intergenic 35.0%
TTCAGTTTCTGCATAACATG+TGG - chr4_3:69094879-69094898 Msa0658900:intron 35.0%
TTGAATTAAGTCTCCTGTTG+CGG - chr4_3:69095485-69095504 Msa0658900:intron 35.0%
!! CTATTTTGGATACTGGCATT+TGG - chr4_3:69095508-69095527 Msa0658900:intron 35.0%
ATTCTTTAGGCAACCCAATA+TGG + chr4_3:69095910-69095929 None:intergenic 35.0%
ACAGACTCATGAGATTCTTT+AGG + chr4_3:69095923-69095942 None:intergenic 35.0%
TGAAGATGGTCAGTTGTATA+TGG - chr4_3:69096002-69096021 Msa0658900:five_prime_UTR 35.0%
! GTTTCATTAGGATTTTGCTC+AGG - chr4_3:69096146-69096165 Msa0658900:five_prime_UTR 35.0%
GTGAGAGAGAAAGAGAATGA+GGG + chr4_3:69092858-69092877 None:intergenic 40.0%
GAAAGAAGGTAGAGGAAGAA+TGG + chr4_3:69092904-69092923 None:intergenic 40.0%
!! GATGGTGGCTTTCTTTTAGT+GGG + chr4_3:69092954-69092973 None:intergenic 40.0%
!!! TGATGGTGGCTTTCTTTTAG+TGG + chr4_3:69092955-69092974 None:intergenic 40.0%
! GTTTTGTAGAGGATGAAGAG+AGG + chr4_3:69092982-69093001 None:intergenic 40.0%
GGTGGTCTCAAAATTGAACT+TGG + chr4_3:69093029-69093048 None:intergenic 40.0%
!! CTGATGATGCTTTGAAACGT+CGG + chr4_3:69093079-69093098 None:intergenic 40.0%
GCATAATGAAAGAATGAGCC+AGG - chr4_3:69093215-69093234 Msa0658900:intron 40.0%
!! ATGCTTTTGGTGGTCTTCAA+TGG - chr4_3:69093476-69093495 Msa0658900:intron 40.0%
!! TGCTTTTGGTGGTCTTCAAT+GGG - chr4_3:69093477-69093496 Msa0658900:intron 40.0%
!! ATTCTGGAATGGTTCACTGT+TGG - chr4_3:69093530-69093549 Msa0658900:intron 40.0%
GCTAAACATGCAGTCATTGT+AGG - chr4_3:69094606-69094625 Msa0658900:intron 40.0%
! TGCTGTTCTATTGGTTAGCA+CGG - chr4_3:69094749-69094768 Msa0658900:intron 40.0%
!!! GTGTTGCACCTGTTTTGTTT+TGG + chr4_3:69095967-69095986 None:intergenic 40.0%
! TCATTAGGATTTTGCTCAGG+AGG - chr4_3:69096149-69096168 Msa0658900:five_prime_UTR 40.0%
! CATTAGGATTTTGCTCAGGA+GGG - chr4_3:69096150-69096169 Msa0658900:five_prime_UTR 40.0%
! ATTAGGATTTTGCTCAGGAG+GGG - chr4_3:69096151-69096170 Msa0658900:five_prime_UTR 40.0%
CGTGAGAGAGAAAGAGAATG+AGG + chr4_3:69092859-69092878 None:intergenic 45.0%
AGAAGGTAGAGGAAGAATGG+TGG + chr4_3:69092901-69092920 None:intergenic 45.0%
! TGAAGAGAGGAAGATGATGG+TGG + chr4_3:69092969-69092988 None:intergenic 45.0%
GGATGAAGAGAGGAAGATGA+TGG + chr4_3:69092972-69092991 None:intergenic 45.0%
! CGACGTTTCAAAGCATCATC+AGG - chr4_3:69093077-69093096 Msa0658900:three_prime_UTR 45.0%
GAACATGGTACTCATGGAAG+AGG - chr4_3:69093688-69093707 Msa0658900:intron 45.0%
CTGCTAACATGAGCAGCATT+CGG - chr4_3:69093824-69093843 Msa0658900:intron 45.0%
! TAAGTCAGCCTGCCTTTTAG+TGG + chr4_3:69094191-69094210 None:intergenic 45.0%
! TGTGTCAGCGTGTCTTTTAG+TGG + chr4_3:69095272-69095291 None:intergenic 45.0%
GTATCCAAAATAGCCGCAAC+AGG + chr4_3:69095501-69095520 None:intergenic 45.0%
!! GTTGCGGCTATTTTGGATAC+TGG - chr4_3:69095501-69095520 Msa0658900:intron 45.0%
! GGTGGTAGGTTTGGTTGTGA+GGG + chr4_3:69092825-69092844 None:intergenic 50.0%
! TGGTGGTAGGTTTGGTTGTG+AGG + chr4_3:69092826-69092845 None:intergenic 50.0%
AGAGAGAGTGGTGGTAGGTT+TGG + chr4_3:69092834-69092853 None:intergenic 50.0%
AATGAGGGAAGAGAGAGTGG+TGG + chr4_3:69092843-69092862 None:intergenic 50.0%
GAGAATGAGGGAAGAGAGAG+TGG + chr4_3:69092846-69092865 None:intergenic 50.0%
! GGCTTTCTTTTAGTGGGAGG+AGG + chr4_3:69092948-69092967 None:intergenic 50.0%
! GGTGGCTTTCTTTTAGTGGG+AGG + chr4_3:69092951-69092970 None:intergenic 50.0%
CAGGACGCACACTTCTTGTA+CGG - chr4_3:69093234-69093253 Msa0658900:intron 50.0%
! GTCTCCTGTTGCGGCTATTT+TGG - chr4_3:69095494-69095513 Msa0658900:intron 50.0%
AGGGAAGAGAGAGTGGTGGT+AGG + chr4_3:69092839-69092858 None:intergenic 55.0%
AATTCCGACGCGGTGGTCAA+TGG + chr4_3:69093050-69093069 None:intergenic 55.0%
! CGTACAAGAAGTGTGCGTCC+TGG + chr4_3:69093236-69093255 None:intergenic 55.0%
GACGCACACTTCTTGTACGG+AGG - chr4_3:69093237-69093256 Msa0658900:intron 55.0%
ACCACCATTGACCACCGCGT+CGG - chr4_3:69093043-69093062 Msa0658900:three_prime_UTR 60.0%
TCGGAGAGCGAATTCCGACG+CGG + chr4_3:69093060-69093079 None:intergenic 60.0%
TCCGACGCGGTGGTCAATGG+TGG + chr4_3:69093047-69093066 None:intergenic 65.0%
GAGAGCGAATTCCGACGCGG+TGG + chr4_3:69093057-69093076 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 69092812 69096275 69092812 ID=Msa0658900;Name=Msa0658900
chr4_3 mRNA 69092812 69096275 69092812 ID=Msa0658900-mRNA-1;Parent=Msa0658900;Name=Msa0658900-mRNA-1;_AED=0.11;_eAED=0.11;_QI=320|1|0.8|1|0|0|5|326|72
chr4_3 exon 69092812 69093230 69092812 ID=Msa0658900-mRNA-1:exon:20247;Parent=Msa0658900-mRNA-1
chr4_3 exon 69095229 69095282 69095229 ID=Msa0658900-mRNA-1:exon:20246;Parent=Msa0658900-mRNA-1
chr4_3 exon 69095589 69095629 69095589 ID=Msa0658900-mRNA-1:exon:20245;Parent=Msa0658900-mRNA-1
chr4_3 exon 69095829 69095892 69095829 ID=Msa0658900-mRNA-1:exon:20244;Parent=Msa0658900-mRNA-1
chr4_3 exon 69095989 69096275 69095989 ID=Msa0658900-mRNA-1:exon:20243;Parent=Msa0658900-mRNA-1
chr4_3 five_prime_UTR 69095989 69096275 69095989 ID=Msa0658900-mRNA-1:five_prime_utr;Parent=Msa0658900-mRNA-1
chr4_3 five_prime_UTR 69095860 69095892 69095860 ID=Msa0658900-mRNA-1:five_prime_utr;Parent=Msa0658900-mRNA-1
chr4_3 CDS 69095829 69095859 69095829 ID=Msa0658900-mRNA-1:cds;Parent=Msa0658900-mRNA-1
chr4_3 CDS 69095589 69095629 69095589 ID=Msa0658900-mRNA-1:cds;Parent=Msa0658900-mRNA-1
chr4_3 CDS 69095229 69095282 69095229 ID=Msa0658900-mRNA-1:cds;Parent=Msa0658900-mRNA-1
chr4_3 CDS 69093138 69093230 69093138 ID=Msa0658900-mRNA-1:cds;Parent=Msa0658900-mRNA-1
chr4_3 three_prime_UTR 69092812 69093137 69092812 ID=Msa0658900-mRNA-1:three_prime_utr;Parent=Msa0658900-mRNA-1
Gene Sequence

>Msa0658900

ATGAGCCAGGACGCACACTTCTTGTACGGAGCAATAACATTGCGATTTTATGCTTTTGGTGGTCTTCAATGGGCGGTTGATTTTATCTCTACTGCTAACATGAGCAGCATTCGGTTGTTATTATTTATTTTCATGATTAGAGAATATATGTTCACTCAAGGTTTGGTAATATTTGTCCATATTGGGTTGCCTAAAGAATCTCATGAGTCTGTGCTTTAG
Protein sequence

>Msa0658900

MSQDAHFLYGAITLRFYAFGGLQWAVDFISTANMSSIRLLLFIFMIREYMFTQGLVIFVHIGLPKESHESVL*