AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0677010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0677010 MtrunA17_Chr4g0020771 95.721 444 19 0 1 444 1 444 0.0 898
Msa0677010 MtrunA17_Chr3g0089021 59.775 445 169 6 1 443 2 438 0.0 521
Msa0677010 MtrunA17_Chr1g0173451 50.455 440 199 6 8 441 9 435 8.30e-145 421
Msa0677010 MtrunA17_Chr2g0332801 46.092 371 193 5 73 439 58 425 5.97e-113 339
Msa0677010 MtrunA17_Chr8g0365901 38.914 442 243 9 7 439 6 429 1.19e-108 328
Msa0677010 MtrunA17_Chr7g0257701 47.368 342 132 3 62 365 53 384 1.64e-101 308
Msa0677010 MtrunA17_Chr7g0257721 49.390 328 151 6 11 330 11 331 5.39e-96 292
Msa0677010 MtrunA17_Chr6g0449961 43.732 343 180 7 76 415 47 379 2.15e-92 286
Msa0677010 MtrunA17_Chr4g0052621 43.251 363 191 7 68 419 59 417 3.89e-90 281
Msa0677010 MtrunA17_Chr7g0270361 40.872 367 207 6 62 419 55 420 7.88e-90 280
Msa0677010 MtrunA17_Chr3g0121881 40.108 369 202 7 70 430 29 386 7.54e-87 270
Msa0677010 MtrunA17_Chr5g0413141 38.043 368 219 7 79 439 64 429 1.43e-86 271
Msa0677010 MtrunA17_Chr2g0303941 41.623 382 205 10 65 440 19 388 1.88e-86 269
Msa0677010 MtrunA17_Chr1g0194231 40.872 367 192 9 70 419 77 435 3.90e-86 271
Msa0677010 MtrunA17_Chr8g0365891 38.360 378 196 7 70 416 103 474 6.12e-84 267
Msa0677010 MtrunA17_Chr4g0043661 37.867 375 205 8 71 430 20 381 1.14e-83 262
Msa0677010 MtrunA17_Chr5g0418411 40.833 360 195 8 79 430 34 383 7.97e-83 260
Msa0677010 MtrunA17_Chr3g0121891 39.496 357 195 7 79 426 34 378 2.75e-81 256
Msa0677010 MtrunA17_Chr2g0332971 40.233 343 197 5 76 412 30 370 1.14e-79 252
Msa0677010 MtrunA17_Chr5g0413411 38.275 371 193 8 76 416 123 487 1.20e-79 256
Msa0677010 MtrunA17_Chr7g0230891 39.353 371 207 10 76 440 39 397 3.14e-77 246
Msa0677010 MtrunA17_Chr4g0067631 37.469 403 230 14 51 443 32 422 6.95e-77 245
Msa0677010 MtrunA17_Chr8g0348981 39.718 355 197 9 73 418 26 372 4.79e-74 237
Msa0677010 MtrunA17_Chr8g0337571 38.606 373 201 9 70 434 27 379 4.21e-73 234
Msa0677010 MtrunA17_Chr8g0337581 38.108 370 202 9 76 437 33 383 1.71e-71 231
Msa0677010 MtrunA17_Chr4g0043431 39.826 344 194 9 76 414 37 372 5.09e-71 229
Msa0677010 MtrunA17_Chr6g0461181 34.901 404 206 9 75 432 31 423 5.43e-71 231
Msa0677010 MtrunA17_Chr7g0228411 36.074 377 206 10 80 428 46 415 6.74e-70 228
Msa0677010 MtrunA17_Chr2g0293681 40.122 329 183 6 68 386 61 385 8.94e-70 227
Msa0677010 MtrunA17_Chr4g0018151 33.482 448 248 10 12 430 1 427 4.79e-69 226
Msa0677010 MtrunA17_Chr2g0307831 34.908 381 237 9 66 441 17 391 3.40e-68 223
Msa0677010 MtrunA17_Chr2g0307821 34.908 381 237 9 66 441 17 391 3.40e-68 223
Msa0677010 MtrunA17_Chr3g0100091 38.542 288 161 5 142 428 59 331 4.69e-66 215
Msa0677010 MtrunA17_Chr2g0291841 37.463 339 202 8 104 440 29 359 6.26e-65 213
Msa0677010 MtrunA17_Chr2g0307811 36.053 380 220 9 73 441 24 391 2.37e-64 213
Msa0677010 MtrunA17_Chr1g0195221 32.911 395 243 11 57 439 18 402 3.29e-63 209
Msa0677010 MtrunA17_Chr2g0295451 36.544 353 189 13 62 393 43 381 6.69e-61 202
Msa0677010 MtrunA17_Chr1g0150291 34.493 345 208 9 74 412 45 377 1.13e-59 200
Msa0677010 MtrunA17_Chr1g0150301 34.783 345 207 9 74 412 27 359 9.07e-59 197
Msa0677010 MtrunA17_Chr5g0425811 36.749 283 162 7 90 367 2 272 2.10e-55 185
Msa0677010 MtrunA17_Chr8g0336481 37.729 273 152 4 131 401 14 270 8.81e-54 181
Msa0677010 MtrunA17_Chr5g0447901 35.443 316 187 9 73 382 24 328 4.25e-52 178
Msa0677010 MtrunA17_Chr3g0093011 41.518 224 128 1 199 419 13 236 9.11e-52 175
Msa0677010 MtrunA17_Chr8g0337601 42.616 237 129 4 202 437 4 234 1.40e-51 174
Msa0677010 MtrunA17_Chr8g0337511 40.260 231 130 3 199 428 4 227 1.46e-51 174
Msa0677010 MtrunA17_Chr8g0337521 35.816 282 156 7 54 329 11 273 2.08e-46 162
Msa0677010 MtrunA17_Chr7g0273191 52.143 140 64 2 174 313 2 138 2.30e-44 152
Msa0677010 MtrunA17_Chr8g0337351 46.667 165 87 1 217 380 8 172 4.12e-42 147
Msa0677010 MtrunA17_Chr1g0149241 29.650 371 213 9 66 430 20 348 3.44e-41 150
Msa0677010 MtrunA17_Chr7g0257711 50.862 116 56 1 330 444 1 116 1.35e-30 114
Msa0677010 MtrunA17_Chr8g0337551 47.656 128 67 0 202 329 7 134 2.16e-30 114
Msa0677010 MtrunA17_Chr4g0067811 24.198 405 230 11 74 429 40 416 1.24e-21 97.1
Msa0677010 MtrunA17_Chr2g0301091 28.958 259 159 7 168 412 169 416 1.62e-20 93.6
Msa0677010 MtrunA17_Chr3g0095221 25.581 387 230 14 74 421 47 414 4.23e-20 92.4
Msa0677010 MtrunA17_Chr5g0442221 27.273 264 169 6 168 419 158 410 2.30e-18 87.0
Msa0677010 MtrunA17_Chr7g0273181 47.500 80 42 0 347 426 1 80 2.55e-18 80.9
Msa0677010 MtrunA17_Chr7g0270821 26.158 367 201 13 74 394 43 385 2.82e-18 87.0
Msa0677010 MtrunA17_Chr1g0193961 26.611 357 195 12 74 386 43 376 2.82e-18 87.0
Msa0677010 MtrunA17_Chr4g0070281 26.255 259 166 7 168 412 188 435 3.24e-18 87.0
Msa0677010 MtrunA17_Chr3g0093021 41.221 131 64 9 68 190 17 142 1.80e-16 77.0
Msa0677010 MtrunA17_Chr7g0255311 23.077 403 250 15 54 415 43 426 3.82e-16 80.5
Msa0677010 MtrunA17_Chr2g0295241 21.975 405 239 10 74 429 37 413 4.14e-16 80.1
Msa0677010 MtrunA17_Chr2g0330221 31.522 184 116 6 67 247 17 193 4.75e-16 76.6
Msa0677010 MtrunA17_Chr3g0130251 25.794 252 163 6 157 394 177 418 6.57e-16 79.7
Msa0677010 MtrunA17_Chr8g0387391 25.633 316 177 11 75 350 45 342 1.59e-15 78.6
Msa0677010 MtrunA17_Chr8g0348491 24.148 352 219 13 74 390 55 393 4.88e-15 77.0
Msa0677010 MtrunA17_Chr8g0373171 41.772 79 46 0 200 278 7 85 5.82e-14 68.6
Msa0677010 MtrunA17_Chr7g0248451 22.573 412 251 13 75 441 56 444 7.01e-14 73.6
Msa0677010 MtrunA17_Chr8g0337541 32.812 128 73 6 58 179 20 140 8.04e-14 68.9
Msa0677010 MtrunA17_Chr1g0202651 22.487 378 238 11 76 415 57 417 1.08e-13 72.8
Msa0677010 MtrunA17_Chr1g0202641 22.222 378 239 14 76 415 57 417 3.16e-12 68.9
Msa0677010 MtrunA17_Chr1g0202641 22.102 371 248 13 76 415 557 917 3.80e-11 65.5
Msa0677010 MtrunA17_Chr8g0337561 39.423 104 56 3 332 434 8 105 4.42e-12 62.8
Msa0677010 MtrunA17_Chr7g0258111 24.444 270 178 5 147 400 123 382 5.66e-12 67.4
Msa0677010 MtrunA17_Chr8g0337531 38.679 106 58 3 330 434 23 122 3.25e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0677010 AT3G07970.1 52.423 454 189 11 1 442 1 439 3.62e-150 434
Msa0677010 AT3G57510.1 48.416 442 200 12 11 442 8 431 1.01e-137 402
Msa0677010 AT2G41850.1 49.433 441 197 12 10 441 7 430 8.78e-127 374
Msa0677010 AT1G80170.1 47.606 376 189 6 69 439 48 420 3.01e-117 350
Msa0677010 AT1G23460.2 45.119 379 199 7 66 439 33 407 2.89e-108 327
Msa0677010 AT1G23460.1 45.119 379 199 7 66 439 57 431 5.19e-108 327
Msa0677010 AT1G70500.1 43.536 379 205 7 66 439 57 431 1.94e-104 318
Msa0677010 AT1G56710.1 43.697 357 184 7 76 419 50 402 3.67e-92 286
Msa0677010 AT3G15720.3 42.703 370 190 8 70 430 19 375 4.85e-92 286
Msa0677010 AT3G15720.1 42.703 370 190 8 70 430 20 376 5.27e-92 286
Msa0677010 AT5G14650.1 40.710 366 202 7 59 419 36 391 6.90e-90 280
Msa0677010 AT3G15720.2 42.432 370 188 9 70 430 19 372 4.51e-89 278
Msa0677010 AT1G48100.1 40.054 367 197 8 68 419 74 432 1.78e-87 275
Msa0677010 AT2G43890.1 39.257 377 217 8 67 440 22 389 3.05e-86 269
Msa0677010 AT4G35670.1 41.111 360 195 7 76 429 26 374 5.60e-86 268
Msa0677010 AT3G26610.1 35.714 420 250 9 6 419 8 413 3.90e-85 269
Msa0677010 AT5G27530.1 39.665 358 191 5 79 430 58 396 2.83e-84 266
Msa0677010 AT1G05650.1 39.362 376 217 7 67 439 21 388 1.45e-83 262
Msa0677010 AT3G15720.4 43.034 323 165 6 114 430 3 312 1.07e-82 260
Msa0677010 AT1G02460.1 41.033 368 189 9 72 419 87 446 1.09e-82 263
Msa0677010 AT1G05660.1 38.992 377 217 8 67 439 21 388 8.62e-82 258
Msa0677010 AT1G60590.1 38.378 370 197 7 76 416 125 492 1.13e-81 262
Msa0677010 AT1G10640.1 38.147 367 199 7 76 416 121 485 1.46e-80 259
Msa0677010 AT1G80140.1 41.195 318 169 7 108 419 6 311 3.55e-80 251
Msa0677010 AT4G18180.1 37.566 378 210 8 73 430 38 409 3.10e-78 249
Msa0677010 AT5G44840.1 37.500 360 209 7 76 429 26 375 1.17e-77 247
Msa0677010 AT1G80170.2 46.816 267 135 5 69 331 48 311 2.36e-77 244
Msa0677010 AT2G43870.1 39.164 383 216 12 65 441 12 383 6.11e-76 242
Msa0677010 AT2G33160.1 39.096 376 213 9 73 439 31 399 8.55e-76 249
Msa0677010 AT5G17200.1 38.760 387 196 9 70 429 35 407 9.91e-76 243
Msa0677010 AT4G01890.1 38.268 358 201 8 76 417 67 420 1.32e-75 244
Msa0677010 AT3G59850.1 40.058 342 190 10 79 414 28 360 2.72e-74 238
Msa0677010 AT2G15460.1 35.884 379 224 9 73 440 30 400 2.83e-74 238
Msa0677010 AT1G43080.1 37.368 380 217 11 73 440 30 400 3.59e-74 238
Msa0677010 AT3G59850.2 39.942 343 191 10 78 414 71 404 4.94e-74 239
Msa0677010 AT2G15450.1 35.714 378 224 9 73 439 30 399 1.40e-73 237
Msa0677010 AT1G02790.1 37.337 383 207 11 76 441 54 420 1.89e-73 237
Msa0677010 AT2G15470.1 35.620 379 225 9 73 440 30 400 5.97e-73 235
Msa0677010 AT1G43090.1 36.800 375 218 9 73 436 30 396 1.63e-72 235
Msa0677010 AT2G26620.1 36.340 377 223 9 73 440 30 398 5.49e-72 233
Msa0677010 AT2G43880.1 38.810 353 201 8 67 414 24 366 1.88e-71 231
Msa0677010 AT2G40310.1 36.340 377 219 11 76 440 33 400 2.94e-71 231
Msa0677010 AT1G43100.1 36.533 375 219 9 73 436 30 396 2.95e-71 232
Msa0677010 AT1G17150.1 37.466 371 217 9 76 439 35 397 3.19e-71 231
Msa0677010 AT1G65570.1 35.162 401 234 10 46 440 14 394 4.17e-71 230
Msa0677010 AT4G35670.2 42.238 277 149 2 153 429 2 267 9.32e-71 226
Msa0677010 AT4G13760.1 35.092 379 227 9 73 440 30 400 1.57e-70 229
Msa0677010 AT3G07820.1 36.314 369 219 9 81 442 28 387 1.79e-70 228
Msa0677010 AT1G78400.1 36.828 372 218 11 76 439 37 399 2.04e-70 228
Msa0677010 AT2G43860.1 38.997 359 203 10 64 414 20 370 2.71e-70 228
Msa0677010 AT5G48140.1 37.607 351 203 8 85 429 33 373 8.00e-70 227
Msa0677010 AT3G07840.1 35.580 371 216 9 73 429 13 374 3.97e-69 225
Msa0677010 AT3G07830.1 35.359 362 219 8 85 440 33 385 5.34e-69 224
Msa0677010 AT3G14040.1 36.997 373 219 9 74 439 71 434 1.68e-67 222
Msa0677010 AT3G07850.1 36.675 379 224 9 68 439 64 433 5.05e-67 221
Msa0677010 AT5G44830.1 36.503 326 185 4 106 429 11 316 6.23e-67 217
Msa0677010 AT4G32380.1 34.677 372 223 8 73 434 25 386 2.16e-66 219
Msa0677010 AT5G14650.2 38.489 278 158 5 59 331 36 305 4.25e-61 201
Msa0677010 AT4G32370.1 37.540 309 171 7 69 365 29 327 7.70e-61 202
Msa0677010 AT2G43880.2 38.511 309 175 8 67 370 24 322 3.30e-58 194
Msa0677010 AT4G32375.1 39.080 261 148 3 153 412 13 263 3.90e-58 197
Msa0677010 AT5G39910.1 34.426 305 169 7 73 369 35 316 2.18e-51 178
Msa0677010 AT4G32380.2 30.811 370 199 8 73 434 25 345 2.27e-51 179
Msa0677010 AT1G19170.1 28.185 259 161 6 168 412 201 448 6.84e-21 95.5
Msa0677010 AT3G62110.1 28.879 232 142 5 168 386 156 377 1.20e-20 94.7
Msa0677010 AT3G62110.2 28.879 232 142 5 168 386 156 377 1.20e-20 94.7
Msa0677010 AT3G42950.1 26.738 374 228 14 73 412 65 426 3.29e-20 93.2
Msa0677010 AT4G23820.1 24.557 395 240 14 74 429 41 416 2.59e-19 90.5
Msa0677010 AT4G33440.1 25.000 392 218 14 73 408 71 442 7.48e-18 86.3
Msa0677010 AT3G06770.2 24.147 381 226 12 74 412 43 402 4.10e-17 83.6
Msa0677010 AT3G16850.1 23.774 265 170 5 168 415 153 402 4.27e-17 83.6
Msa0677010 AT3G06770.5 24.147 381 226 12 74 412 44 403 4.43e-17 83.6
Msa0677010 AT2G23900.1 25.000 316 178 10 76 350 58 355 6.33e-17 83.2
Msa0677010 AT5G41870.1 24.859 354 208 14 73 386 44 379 1.04e-16 82.4
Msa0677010 AT3G48950.1 23.919 347 199 11 47 350 20 344 1.71e-16 82.0
Msa0677010 AT5G49215.2 23.438 384 231 11 74 415 41 403 1.89e-16 81.6
Msa0677010 AT5G49215.1 23.438 384 231 11 74 415 43 405 1.96e-16 81.6
Msa0677010 AT4G23500.1 25.316 316 178 11 75 350 69 366 1.02e-15 79.7
Msa0677010 AT3G06770.1 24.031 258 172 5 168 412 87 333 4.31e-15 77.0
Msa0677010 AT3G06770.3 24.031 258 172 5 168 412 87 333 4.31e-15 77.0
Msa0677010 AT3G06770.4 24.031 258 172 5 168 412 69 315 4.86e-15 76.6
Msa0677010 AT3G61490.4 30.208 192 113 6 168 346 271 454 7.31e-14 73.9
Msa0677010 AT3G61490.5 30.208 192 113 6 168 346 173 356 7.49e-14 73.9
Msa0677010 AT3G61490.2 30.366 191 112 6 168 345 158 340 8.54e-14 73.6
Msa0677010 AT3G61490.3 30.366 191 112 6 168 345 158 340 8.54e-14 73.6
Msa0677010 AT3G61490.1 30.366 191 112 6 168 345 158 340 8.54e-14 73.6
Msa0677010 AT3G57790.2 28.037 214 139 5 74 272 35 248 4.71e-11 65.1
Msa0677010 AT3G57790.1 28.037 214 139 5 74 272 35 248 4.71e-11 65.1

Find 89 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACCAAATACTGCCTTTA+TGG 0.149151 4_4:+29105258 Msa0677010:CDS
GACATAATAAACTGTGATAT+TGG 0.217261 4_4:+29106298 Msa0677010:CDS
CTCACCATAAAGGCAGTATT+TGG 0.245870 4_4:-29105262 None:intergenic
AAAACAAAACATCATCATTT+TGG 0.257008 4_4:+29104906 Msa0677010:CDS
AATTCATCATGTTAGCTCTA+TGG 0.292346 4_4:+29105601 Msa0677010:intron
GTAGTTCCTCCACCACCTTC+TGG 0.295016 4_4:-29108024 None:intergenic
TTCTGCAGTATTGGAAGTTT+AGG 0.326431 4_4:+29107168 Msa0677010:intron
GTTATTGTTGGAATATTTGC+AGG 0.329378 4_4:-29105761 None:intergenic
ATTTGAGAGTAAAGAATCTA+AGG 0.331559 4_4:+29106143 Msa0677010:CDS
GAGATGGTATATCCTATTCT+TGG 0.332828 4_4:-29105209 None:intergenic
TTGCAGAGAAATATACTTGC+AGG 0.334080 4_4:+29107987 Msa0677010:CDS
GCACGGCTTTGTTCCGCTCT+TGG 0.338883 4_4:-29107879 None:intergenic
GTAGTATTGATCTATGATTA+TGG 0.353266 4_4:-29107836 None:intergenic
ATCATGAGAAGTGTGATAAT+TGG 0.354858 4_4:-29104780 None:intergenic
GTGAACAGGGCTGCCTTAAC+AGG 0.368388 4_4:+29107228 Msa0677010:CDS
GTCTCAAATTTCAAAGTTGA+TGG 0.380238 4_4:+29105694 Msa0677010:CDS
GCTCTTGGCATGGCTCTGTT+TGG 0.383793 4_4:-29107864 None:intergenic
GCGGACCCGGACATGGAATC+AGG 0.384459 4_4:+29106507 Msa0677010:CDS
AAAACTTACAAGGTTATTGT+TGG 0.389616 4_4:-29105773 None:intergenic
TCCATTAGTTGTTCCTGTTA+AGG 0.393409 4_4:-29107241 None:intergenic
ACATAATAAACTGTGATATT+GGG 0.395546 4_4:+29106299 Msa0677010:CDS
TTTCCAATGTAGTAGTGAAC+AGG 0.405039 4_4:+29107214 Msa0677010:CDS
CTCCTTTAAGGCATTTAAGA+AGG 0.412653 4_4:+29105144 Msa0677010:intron
ATATGATCACAGGGTGGTTC+AGG 0.430537 4_4:+29107760 Msa0677010:intron
ATGAAACCTGATTCCATGTC+CGG 0.431003 4_4:-29106513 None:intergenic
GCCTTAACAGGAACAACTAA+TGG 0.431469 4_4:+29107240 Msa0677010:CDS
TGTCAGCATATGAAGAAGAT+AGG 0.435363 4_4:+29105647 Msa0677010:CDS
TATGATCTTACAGTTGGTCT+TGG 0.435649 4_4:-29106016 None:intergenic
GATTGTATTTCGATAGTTAG+TGG 0.441436 4_4:+29106452 Msa0677010:CDS
TAGTATTGATCTATGATTAT+GGG 0.445220 4_4:-29107835 None:intergenic
TCCTCAATGCTCTCCACAAT+AGG 0.452988 4_4:+29108092 Msa0677010:CDS
GATGGTGGTGGCACTTTCAA+TGG 0.457262 4_4:+29105712 Msa0677010:CDS
AGATGGTATATCCTATTCTT+GGG 0.459795 4_4:-29105208 None:intergenic
TAGTGTAAGACTCACCATAA+AGG 0.459802 4_4:-29105272 None:intergenic
CACATGAATTCCATCGGTGT+TGG 0.469737 4_4:-29106257 None:intergenic
GCTTTGTTCCGCTCTTGGCA+TGG 0.478055 4_4:-29107874 None:intergenic
CAAAACTTGGCTTAATTGCA+CGG 0.479353 4_4:-29107896 None:intergenic
ACATGAATTCCATCGGTGTT+GGG 0.481545 4_4:-29106256 None:intergenic
ACAATATCCATTGCCTATTG+TGG 0.484295 4_4:-29108105 None:intergenic
TGGAATGAAGCATGTTCTAA+AGG 0.492280 4_4:+29105169 Msa0677010:CDS
AGCCCTGTTCACTACTACAT+TGG 0.494296 4_4:-29107217 None:intergenic
CTCTTCAAAGTCTCAAGGAA+TGG 0.495111 4_4:+29104962 Msa0677010:CDS
GGAATGAAGCATGTTCTAAA+GGG 0.497809 4_4:+29105170 Msa0677010:CDS
ATGGTTAGTGTTGATGACTT+TGG 0.500293 4_4:+29104981 Msa0677010:CDS
AGATGGAAGAGATGATAGAG+AGG 0.500756 4_4:+29105013 Msa0677010:CDS
GACTTTGGAGCAAGAGCAGA+TGG 0.502754 4_4:+29104996 Msa0677010:CDS
TCCTTGTGGTGCCCAAGAAT+AGG 0.505871 4_4:+29105197 Msa0677010:CDS
TCGGTGTTGGGACTATCCTC+TGG 0.506883 4_4:-29106244 None:intergenic
AATTTCAAAGTTGATGGTGG+TGG 0.508006 4_4:+29105700 Msa0677010:CDS
TTTCTCTGCAAGGAACGCTT+CGG 0.508888 4_4:-29107975 None:intergenic
TTAAGGCATTTAAGAAGGCA+TGG 0.511868 4_4:+29105149 Msa0677010:intron
ATAGATATCACTTGCGGACC+CGG 0.513667 4_4:+29106494 Msa0677010:CDS
TCAACATTGGCTTGATGAAG+TGG 0.522002 4_4:-29104849 None:intergenic
GTGGCACTTTCAATGGCAAT+GGG 0.535626 4_4:+29105719 Msa0677010:CDS
ATCACTTGCGGACCCGGACA+TGG 0.542140 4_4:+29106500 Msa0677010:CDS
GGTGGCACTTTCAATGGCAA+TGG 0.545089 4_4:+29105718 Msa0677010:CDS
GGTCTTGGTTCATCATTGCA+TGG 0.546282 4_4:-29106001 None:intergenic
ATGCAATGGCTCTTACATAG+TGG 0.549275 4_4:+29104824 Msa0677010:CDS
ATGAAGAAGATAGGCAACAC+TGG 0.549453 4_4:+29105656 Msa0677010:CDS
ATAAACTGTGATATTGGGAC+AGG 0.552284 4_4:+29106304 Msa0677010:CDS
TCAATCTACCATCATGTACA+TGG 0.568484 4_4:-29104881 None:intergenic
AATGTTAGATATGTAAATAG+AGG 0.571277 4_4:+29108060 Msa0677010:CDS
TTCAGCCACATGAATTCCAT+CGG 0.571554 4_4:-29106263 None:intergenic
TTCTAAAGGGTCTATCCTTG+TGG 0.572564 4_4:+29105183 Msa0677010:CDS
TGAAACCTGATTCCATGTCC+GGG 0.576277 4_4:-29106512 None:intergenic
CTAGAACCAGAAGGTGGTGG+AGG 0.582001 4_4:+29108018 Msa0677010:CDS
GCCTATTGTGGAGAGCATTG+AGG 0.588158 4_4:-29108093 None:intergenic
TGCAAGTATATTTCTCTGCA+AGG 0.588447 4_4:-29107985 None:intergenic
GAACATAGAAATGCAAAATG+TGG 0.591101 4_4:+29107807 Msa0677010:CDS
ATTCTAGAACCAGAAGGTGG+TGG 0.593437 4_4:+29108015 Msa0677010:CDS
AAGGCAGTATTTGGTTGACA+TGG 0.594353 4_4:-29105253 None:intergenic
AATGCTACATGCATTTGTTG+TGG 0.595049 4_4:-29106175 None:intergenic
TGCATATGAGTAATGAAGCT+AGG 0.596335 4_4:-29104806 None:intergenic
GAGGATAGTCCCAACACCGA+TGG 0.596952 4_4:+29106247 Msa0677010:CDS
CGTTACTACCATGTACATGA+TGG 0.600317 4_4:+29104873 Msa0677010:CDS
TCCTATTCTTGGGCACCACA+AGG 0.600648 4_4:-29105198 None:intergenic
TTAGCTCTATGGAACAATTG+AGG 0.602214 4_4:+29105612 Msa0677010:intron
ATGGTAGTAACGTTCAACAT+TGG 0.605356 4_4:-29104862 None:intergenic
TTTGATTGTGAGAGCGCCAG+AGG 0.607314 4_4:+29106228 Msa0677010:CDS
AAACAGAGCCATGCCAAGAG+CGG 0.620744 4_4:+29107866 Msa0677010:CDS
GCTTCATTACTCATATGCAA+TGG 0.623445 4_4:+29104810 Msa0677010:CDS
TCAAATTTCAAAGTTGATGG+TGG 0.632469 4_4:+29105697 Msa0677010:CDS
TCTATGGAACAATTGAGGCA+TGG 0.634765 4_4:+29105617 Msa0677010:CDS
TGAAGAAGATAGGCAACACT+GGG 0.645980 4_4:+29105657 Msa0677010:CDS
CAACACCGATGGAATTCATG+TGG 0.652375 4_4:+29106258 Msa0677010:CDS
TTCCAATGTAGTAGTGAACA+GGG 0.653388 4_4:+29107215 Msa0677010:CDS
CGAGCTATAGATATCACTTG+CGG 0.678372 4_4:+29106488 Msa0677010:CDS
GTGATTCTAGAACCAGAAGG+TGG 0.703740 4_4:+29108012 Msa0677010:CDS
GATGTGATTCTAGAACCAGA+AGG 0.739033 4_4:+29108009 Msa0677010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAACAATAACATATA+TGG + chr4_4:29105038-29105057 Msa0677010:intron 10.0%
!! AAAATATAACAAATGAAAAT+TGG - chr4_4:29105819-29105838 None:intergenic 10.0%
!! ACATTCAAAAAATAAATATT+TGG + chr4_4:29106362-29106381 Msa0677010:intron 10.0%
!!! AAAAGAATGATTTCAAATTT+TGG + chr4_4:29105875-29105894 Msa0677010:intron 15.0%
!!! ATACTTGTTGTAAAAATTTA+TGG - chr4_4:29106051-29106070 None:intergenic 15.0%
!! AATGGAAAATAAATTTATGT+AGG + chr4_4:29106096-29106115 Msa0677010:intron 15.0%
!! CTAAATAAAAAACATTCAAT+TGG + chr4_4:29106388-29106407 Msa0677010:intron 15.0%
!!! ATTGATTAAAACTTTTTCTT+TGG + chr4_4:29106601-29106620 Msa0677010:intron 15.0%
!!! TCTAACATATATTTTTTTTG+AGG + chr4_4:29106712-29106731 Msa0677010:intron 15.0%
!!! CTAACATATATTTTTTTTGA+GGG + chr4_4:29106713-29106732 Msa0677010:intron 15.0%
!! AATTTCAACATATATGAAAA+AGG + chr4_4:29106740-29106759 Msa0677010:intron 15.0%
!! ATTTCAACATATATGAAAAA+GGG + chr4_4:29106741-29106760 Msa0677010:intron 15.0%
!!! ATTATATTTGACTTTTTAAC+CGG + chr4_4:29106875-29106894 Msa0677010:intron 15.0%
!! ATATAAAAAAATGTCTTAAC+CGG - chr4_4:29106913-29106932 None:intergenic 15.0%
!!! GTTAAGACATTTTTTTATAT+GGG + chr4_4:29106913-29106932 Msa0677010:intron 15.0%
!! ATTTAGAAGAAATTAATAAG+TGG - chr4_4:29107606-29107625 None:intergenic 15.0%
!! TTTCTATGGTTATTAATTAA+AGG - chr4_4:29107642-29107661 None:intergenic 15.0%
!!! AAAACAAAACATCATCATTT+TGG + chr4_4:29104906-29104925 Msa0677010:CDS 20.0%
!!! TTCATTTAATTTCTCCTTTA+AGG + chr4_4:29105132-29105151 Msa0677010:intron 20.0%
!!! TCAGTAATGCAATTTTAAAT+TGG - chr4_4:29105317-29105336 None:intergenic 20.0%
!!! TAATGAATTGATTTTGAACA+TGG - chr4_4:29105563-29105582 None:intergenic 20.0%
!! AAATTGGAAAGAGAAAAAAA+AGG - chr4_4:29105803-29105822 None:intergenic 20.0%
!!! CTAATTGATTCATACTTTAA+TGG + chr4_4:29106078-29106097 Msa0677010:intron 20.0%
!! ACATAATAAACTGTGATATT+GGG + chr4_4:29106299-29106318 Msa0677010:CDS 20.0%
!!! TTTACTTTCTTTTTTGTTGA+AGG + chr4_4:29106425-29106444 Msa0677010:intron 20.0%
!!! ATTAAAACTTTTTCTTTGGA+GGG + chr4_4:29106605-29106624 Msa0677010:intron 20.0%
!!! TAAAACTTTAAAGTTGAGAT+TGG + chr4_4:29106637-29106656 Msa0677010:intron 20.0%
!!! AAAACTTTAAAGTTGAGATT+GGG + chr4_4:29106638-29106657 Msa0677010:intron 20.0%
!!! GGTTAAGACATTTTTTTATA+TGG + chr4_4:29106912-29106931 Msa0677010:intron 20.0%
!! CAATGTAATATTACTACATT+TGG + chr4_4:29107044-29107063 Msa0677010:intron 20.0%
!! CAGAAAAGGAAATATAAAAA+GGG - chr4_4:29107115-29107134 None:intergenic 20.0%
!! TAATATATATTCTGCAGTAT+TGG + chr4_4:29107159-29107178 Msa0677010:intron 20.0%
!!! ATTTTGAAAATTAGTCTGTT+AGG + chr4_4:29107303-29107322 Msa0677010:intron 20.0%
!!! TAGTATTGATCTATGATTAT+GGG - chr4_4:29107838-29107857 None:intergenic 20.0%
!! AATGTTAGATATGTAAATAG+AGG + chr4_4:29108060-29108079 Msa0677010:CDS 20.0%
! CGTAATTAACTGAATTATTG+AGG - chr4_4:29105426-29105445 None:intergenic 25.0%
! TTCAGTTAATTACGTTACTA+TGG + chr4_4:29105432-29105451 Msa0677010:intron 25.0%
! TTACGTTACTATGGATAAAT+CGG + chr4_4:29105441-29105460 Msa0677010:intron 25.0%
! TTACTTTGTGAACTAATCTA+TGG - chr4_4:29105490-29105509 None:intergenic 25.0%
!! TTGATTTTGAACATGGTATA+AGG - chr4_4:29105556-29105575 None:intergenic 25.0%
! AAAACTTACAAGGTTATTGT+TGG - chr4_4:29105776-29105795 None:intergenic 25.0%
!! AAAAGGTACAAAAACTTACA+AGG - chr4_4:29105786-29105805 None:intergenic 25.0%
! TTATGAAAAATACTTGAGAG+AGG + chr4_4:29105852-29105871 Msa0677010:intron 25.0%
! GGAAAATAAATTTATGTAGG+CGG + chr4_4:29106099-29106118 Msa0677010:intron 25.0%
! ATTTGAGAGTAAAGAATCTA+AGG + chr4_4:29106143-29106162 Msa0677010:CDS 25.0%
! GACATAATAAACTGTGATAT+TGG + chr4_4:29106298-29106317 Msa0677010:CDS 25.0%
! CTCAATCTAAGAATTAAATG+AGG - chr4_4:29106556-29106575 None:intergenic 25.0%
!!! GATTAAAACTTTTTCTTTGG+AGG + chr4_4:29106604-29106623 Msa0677010:intron 25.0%
! CATTGAAACTTATGCATTTA+AGG - chr4_4:29107029-29107048 None:intergenic 25.0%
! CCAGAAAAGGAAATATAAAA+AGG - chr4_4:29107116-29107135 None:intergenic 25.0%
!! CCTTTTTATATTTCCTTTTC+TGG + chr4_4:29107113-29107132 Msa0677010:intron 25.0%
!! TAAGCTATTTGCCAAATTTT+AGG + chr4_4:29107439-29107458 Msa0677010:intron 25.0%
! ATAACCGATAAAACAAACAT+TGG + chr4_4:29107465-29107484 Msa0677010:intron 25.0%
!!! CTTTCCAATGTTTGTTTTAT+CGG - chr4_4:29107472-29107491 None:intergenic 25.0%
!!! GTATTTTGGTCAATTTGTAT+GGG - chr4_4:29107696-29107715 None:intergenic 25.0%
!!! AGTATTTTGGTCAATTTGTA+TGG - chr4_4:29107697-29107716 None:intergenic 25.0%
! ATATTGTAATAAAACTCACG+TGG - chr4_4:29107720-29107739 None:intergenic 25.0%
!!! GTAGTATTGATCTATGATTA+TGG - chr4_4:29107839-29107858 None:intergenic 25.0%
!!! TGATGTTTTTGTACAAAACT+TGG - chr4_4:29107912-29107931 None:intergenic 25.0%
! ATCATGAGAAGTGTGATAAT+TGG - chr4_4:29104783-29104802 None:intergenic 30.0%
!! ATCATTTTGGTCACATAACT+AGG + chr4_4:29104919-29104938 Msa0677010:CDS 30.0%
ACATATATGGCAACAAAAAC+AGG + chr4_4:29105051-29105070 Msa0677010:intron 30.0%
AGATGGTATATCCTATTCTT+GGG - chr4_4:29105211-29105230 None:intergenic 30.0%
!! AAAAAGTTATTGGCTTGAGA+TGG - chr4_4:29105228-29105247 None:intergenic 30.0%
TTCACAAAGTAAAGCTTAGT+GGG + chr4_4:29105498-29105517 Msa0677010:intron 30.0%
! ATTTTGAACATGGTATAAGG+AGG - chr4_4:29105553-29105572 None:intergenic 30.0%
!! AATTCATCATGTTAGCTCTA+TGG + chr4_4:29105601-29105620 Msa0677010:intron 30.0%
GTCTCAAATTTCAAAGTTGA+TGG + chr4_4:29105694-29105713 Msa0677010:CDS 30.0%
!! TCAAATTTCAAAGTTGATGG+TGG + chr4_4:29105697-29105716 Msa0677010:CDS 30.0%
GTTATTGTTGGAATATTTGC+AGG - chr4_4:29105764-29105783 None:intergenic 30.0%
GAAGAATATGATCTTACAGT+TGG - chr4_4:29106025-29106044 None:intergenic 30.0%
GATTGTATTTCGATAGTTAG+TGG + chr4_4:29106452-29106471 Msa0677010:CDS 30.0%
! ATCTATAGCTCGAACATTTT+TGG - chr4_4:29106480-29106499 None:intergenic 30.0%
ATGGAGTGAGAATAAAAACT+TGG + chr4_4:29107259-29107278 Msa0677010:CDS 30.0%
TTATGATTCCATATGATCAC+AGG + chr4_4:29107750-29107769 Msa0677010:intron 30.0%
TATGATTCCATATGATCACA+GGG + chr4_4:29107751-29107770 Msa0677010:intron 30.0%
GAACATAGAAATGCAAAATG+TGG + chr4_4:29107807-29107826 Msa0677010:CDS 30.0%
! GTTTTGTACAAAAACATCAG+AGG + chr4_4:29107913-29107932 Msa0677010:CDS 30.0%
TGCATATGAGTAATGAAGCT+AGG - chr4_4:29104809-29104828 None:intergenic 35.0%
GCTTCATTACTCATATGCAA+TGG + chr4_4:29104810-29104829 Msa0677010:CDS 35.0%
ATGGTAGTAACGTTCAACAT+TGG - chr4_4:29104865-29104884 None:intergenic 35.0%
TCAATCTACCATCATGTACA+TGG - chr4_4:29104884-29104903 None:intergenic 35.0%
! CTTTTCTCTTCAAAGTCTCA+AGG + chr4_4:29104957-29104976 Msa0677010:CDS 35.0%
!! ATGGTTAGTGTTGATGACTT+TGG + chr4_4:29104981-29105000 Msa0677010:CDS 35.0%
!! CTCCTTTAAGGCATTTAAGA+AGG + chr4_4:29105144-29105163 Msa0677010:intron 35.0%
TGCCTTCTTAAATGCCTTAA+AGG - chr4_4:29105149-29105168 None:intergenic 35.0%
! TTAAGGCATTTAAGAAGGCA+TGG + chr4_4:29105149-29105168 Msa0677010:intron 35.0%
TGGAATGAAGCATGTTCTAA+AGG + chr4_4:29105169-29105188 Msa0677010:CDS 35.0%
! GGAATGAAGCATGTTCTAAA+GGG + chr4_4:29105170-29105189 Msa0677010:CDS 35.0%
GAGATGGTATATCCTATTCT+TGG - chr4_4:29105212-29105231 None:intergenic 35.0%
!! CTCAAGCCAATAACTTTTTC+AGG + chr4_4:29105229-29105248 Msa0677010:CDS 35.0%
CATGGACCTGAAAAAGTTAT+TGG - chr4_4:29105238-29105257 None:intergenic 35.0%
TCAACCAAATACTGCCTTTA+TGG + chr4_4:29105258-29105277 Msa0677010:CDS 35.0%
TAGTGTAAGACTCACCATAA+AGG - chr4_4:29105275-29105294 None:intergenic 35.0%
! GGTTTTTGTTGCATTACTGA+AGG - chr4_4:29105376-29105395 None:intergenic 35.0%
ACCCTCAATAACATTACTGA+CGG + chr4_4:29105393-29105412 Msa0677010:intron 35.0%
TCCGTCAGTAATGTTATTGA+GGG - chr4_4:29105397-29105416 None:intergenic 35.0%
TTCCGTCAGTAATGTTATTG+AGG - chr4_4:29105398-29105417 None:intergenic 35.0%
CAGATTTGTCAATTGAGTAC+TGG - chr4_4:29105467-29105486 None:intergenic 35.0%
GTTCACAAAGTAAAGCTTAG+TGG + chr4_4:29105497-29105516 Msa0677010:intron 35.0%
!! TTAGCTCTATGGAACAATTG+AGG + chr4_4:29105612-29105631 Msa0677010:intron 35.0%
TGTCAGCATATGAAGAAGAT+AGG + chr4_4:29105647-29105666 Msa0677010:CDS 35.0%
!! AATTTCAAAGTTGATGGTGG+TGG + chr4_4:29105700-29105719 Msa0677010:CDS 35.0%
TCAATGGCAATGGGAAAAAA+TGG + chr4_4:29105728-29105747 Msa0677010:CDS 35.0%
! TATGATCTTACAGTTGGTCT+TGG - chr4_4:29106019-29106038 None:intergenic 35.0%
! AATGCTACATGCATTTGTTG+TGG - chr4_4:29106178-29106197 None:intergenic 35.0%
ATAAACTGTGATATTGGGAC+AGG + chr4_4:29106304-29106323 Msa0677010:CDS 35.0%
TGAAAAAGGGTAATGCTAAC+CGG + chr4_4:29106754-29106773 Msa0677010:intron 35.0%
CTCTATAAGTAGTCCAGAAA+AGG - chr4_4:29107129-29107148 None:intergenic 35.0%
! TTCTGCAGTATTGGAAGTTT+AGG + chr4_4:29107168-29107187 Msa0677010:intron 35.0%
TTTCCAATGTAGTAGTGAAC+AGG + chr4_4:29107214-29107233 Msa0677010:CDS 35.0%
TTCCAATGTAGTAGTGAACA+GGG + chr4_4:29107215-29107234 Msa0677010:CDS 35.0%
TCCATTAGTTGTTCCTGTTA+AGG - chr4_4:29107244-29107263 None:intergenic 35.0%
AGTGAGAATAAAAACTTGGC+AGG + chr4_4:29107263-29107282 Msa0677010:CDS 35.0%
AAAACTTGGCAGGTAAAAGT+TGG + chr4_4:29107273-29107292 Msa0677010:intron 35.0%
TCGGTTATGCACCTAAAATT+TGG - chr4_4:29107453-29107472 None:intergenic 35.0%
TTGACAAATCTCCACTTTGT+TGG + chr4_4:29107516-29107535 Msa0677010:intron 35.0%
TGAAAAAAGGACCAACAAAG+TGG - chr4_4:29107530-29107549 None:intergenic 35.0%
!! ACGGTTTGGTCAATGAAAAA+AGG - chr4_4:29107543-29107562 None:intergenic 35.0%
GTTAGGTAGTTTGAACTAGA+CGG - chr4_4:29107562-29107581 None:intergenic 35.0%
AAGTGGATAAAACTTGTCAC+AGG - chr4_4:29107589-29107608 None:intergenic 35.0%
! AGGAGAAGAGCAATTTTCTA+TGG - chr4_4:29107656-29107675 None:intergenic 35.0%
!! AAAACTCACGTGGAGTATTT+TGG - chr4_4:29107710-29107729 None:intergenic 35.0%
CAAAACTTGGCTTAATTGCA+CGG - chr4_4:29107899-29107918 None:intergenic 35.0%
TGCAAGTATATTTCTCTGCA+AGG - chr4_4:29107988-29108007 None:intergenic 35.0%
TTGCAGAGAAATATACTTGC+AGG + chr4_4:29107987-29108006 Msa0677010:CDS 35.0%
ATGCAATGGCTCTTACATAG+TGG + chr4_4:29104824-29104843 Msa0677010:CDS 40.0%
!! TCAACATTGGCTTGATGAAG+TGG - chr4_4:29104852-29104871 None:intergenic 40.0%
CGTTACTACCATGTACATGA+TGG + chr4_4:29104873-29104892 Msa0677010:CDS 40.0%
CTCTTCAAAGTCTCAAGGAA+TGG + chr4_4:29104962-29104981 Msa0677010:CDS 40.0%
AGATGGAAGAGATGATAGAG+AGG + chr4_4:29105013-29105032 Msa0677010:CDS 40.0%
! TTCTAAAGGGTCTATCCTTG+TGG + chr4_4:29105183-29105202 Msa0677010:CDS 40.0%
AAGGCAGTATTTGGTTGACA+TGG - chr4_4:29105256-29105275 None:intergenic 40.0%
CTCACCATAAAGGCAGTATT+TGG - chr4_4:29105265-29105284 None:intergenic 40.0%
AGACCCTCTGTAATTACTGA+CGG + chr4_4:29105344-29105363 Msa0677010:intron 40.0%
AGACCGTCAGTAATTACAGA+GGG - chr4_4:29105350-29105369 None:intergenic 40.0%
TAGACCGTCAGTAATTACAG+AGG - chr4_4:29105351-29105370 None:intergenic 40.0%
TCTATGGAACAATTGAGGCA+TGG + chr4_4:29105617-29105636 Msa0677010:CDS 40.0%
ATGAAGAAGATAGGCAACAC+TGG + chr4_4:29105656-29105675 Msa0677010:CDS 40.0%
TGAAGAAGATAGGCAACACT+GGG + chr4_4:29105657-29105676 Msa0677010:CDS 40.0%
ATGGCAATGGGAAAAAATGG+TGG + chr4_4:29105731-29105750 Msa0677010:CDS 40.0%
CTCCTCTCTAATTTCGTATG+AGG - chr4_4:29105901-29105920 None:intergenic 40.0%
! ACATGAATTCCATCGGTGTT+GGG - chr4_4:29106259-29106278 None:intergenic 40.0%
TTCAGCCACATGAATTCCAT+CGG - chr4_4:29106266-29106285 None:intergenic 40.0%
CGAGCTATAGATATCACTTG+CGG + chr4_4:29106488-29106507 Msa0677010:CDS 40.0%
ATGAAACCTGATTCCATGTC+CGG - chr4_4:29106516-29106535 None:intergenic 40.0%
!! TGTCTCTAGGGCATTAGTTA+AGG + chr4_4:29106777-29106796 Msa0677010:intron 40.0%
GCCTTAACAGGAACAACTAA+TGG + chr4_4:29107240-29107259 Msa0677010:CDS 40.0%
GTAGTTTGAACTAGACGGTT+TGG - chr4_4:29107557-29107576 None:intergenic 40.0%
AACTTGTCACAGGAGTTGTT+AGG - chr4_4:29107579-29107598 None:intergenic 40.0%
!! GATGTGATTCTAGAACCAGA+AGG + chr4_4:29108009-29108028 Msa0677010:CDS 40.0%
GTGGCACTTTCAATGGCAAT+GGG + chr4_4:29105719-29105738 Msa0677010:CDS 45.0%
GGCCTCATACGAAATTAGAG+AGG + chr4_4:29105896-29105915 Msa0677010:intron 45.0%
GGCTGTGTTCAACAAGAAGA+GGG - chr4_4:29105983-29106002 None:intergenic 45.0%
TGGCTGTGTTCAACAAGAAG+AGG - chr4_4:29105984-29106003 None:intergenic 45.0%
GGTCTTGGTTCATCATTGCA+TGG - chr4_4:29106004-29106023 None:intergenic 45.0%
CACATGAATTCCATCGGTGT+TGG - chr4_4:29106260-29106279 None:intergenic 45.0%
CAACACCGATGGAATTCATG+TGG + chr4_4:29106258-29106277 Msa0677010:CDS 45.0%
ATAGATATCACTTGCGGACC+CGG + chr4_4:29106494-29106513 Msa0677010:CDS 45.0%
TGAAACCTGATTCCATGTCC+GGG - chr4_4:29106515-29106534 None:intergenic 45.0%
TAATGCTAACCGGTGTCTCT+AGG + chr4_4:29106764-29106783 Msa0677010:intron 45.0%
AATGCTAACCGGTGTCTCTA+GGG + chr4_4:29106765-29106784 Msa0677010:intron 45.0%
AACTAATGCCCTAGAGACAC+CGG - chr4_4:29106776-29106795 None:intergenic 45.0%
GGAAATGTTAACTAGTGCCC+TGG - chr4_4:29107094-29107113 None:intergenic 45.0%
AGCCCTGTTCACTACTACAT+TGG - chr4_4:29107220-29107239 None:intergenic 45.0%
!!! GGGAGTGAGCGTTTTTTGTT+AGG - chr4_4:29107676-29107695 None:intergenic 45.0%
GATTCCATATGATCACAGGG+TGG + chr4_4:29107754-29107773 Msa0677010:intron 45.0%
TGAACCACCCTGTGATCATA+TGG - chr4_4:29107761-29107780 None:intergenic 45.0%
ATATGATCACAGGGTGGTTC+AGG + chr4_4:29107760-29107779 Msa0677010:intron 45.0%
TTTCTCTGCAAGGAACGCTT+CGG - chr4_4:29107978-29107997 None:intergenic 45.0%
!! GTGATTCTAGAACCAGAAGG+TGG + chr4_4:29108012-29108031 Msa0677010:CDS 45.0%
!! ATTCTAGAACCAGAAGGTGG+TGG + chr4_4:29108015-29108034 Msa0677010:CDS 45.0%
!! GACTTTGGAGCAAGAGCAGA+TGG + chr4_4:29104996-29105015 Msa0677010:CDS 50.0%
TCCTTGTGGTGCCCAAGAAT+AGG + chr4_4:29105197-29105216 Msa0677010:CDS 50.0%
TCCTATTCTTGGGCACCACA+AGG - chr4_4:29105201-29105220 None:intergenic 50.0%
!! GATGGTGGTGGCACTTTCAA+TGG + chr4_4:29105712-29105731 Msa0677010:CDS 50.0%
GGTGGCACTTTCAATGGCAA+TGG + chr4_4:29105718-29105737 Msa0677010:CDS 50.0%
!! TTTGATTGTGAGAGCGCCAG+AGG + chr4_4:29106228-29106247 Msa0677010:CDS 50.0%
! ACTTTTTAACCGGTGCCCAG+AGG + chr4_4:29106885-29106904 Msa0677010:intron 50.0%
ATGTCTTAACCGGTGCCTCT+GGG - chr4_4:29106903-29106922 None:intergenic 50.0%
AATGTCTTAACCGGTGCCTC+TGG - chr4_4:29106904-29106923 None:intergenic 50.0%
!! GGTCATTTTAACCAGTGCCC+CGG + chr4_4:29106934-29106953 Msa0677010:intron 50.0%
!! GTCATTTTAACCAGTGCCCC+GGG + chr4_4:29106935-29106954 Msa0677010:intron 50.0%
!! TCATTTTAACCAGTGCCCCG+GGG + chr4_4:29106936-29106955 Msa0677010:intron 50.0%
AAACAGAGCCATGCCAAGAG+CGG + chr4_4:29107866-29107885 Msa0677010:CDS 50.0%
!! TCGGTGTTGGGACTATCCTC+TGG - chr4_4:29106247-29106266 None:intergenic 55.0%
GAGGATAGTCCCAACACCGA+TGG + chr4_4:29106247-29106266 Msa0677010:CDS 55.0%
!! CATTTTAACCAGTGCCCCGG+GGG + chr4_4:29106937-29106956 Msa0677010:intron 55.0%
ACTTCCTTAACCAGTGCCCC+CGG - chr4_4:29106956-29106975 None:intergenic 55.0%
GCTTCCTTAACCAGTGCCCT+GGG + chr4_4:29107066-29107085 Msa0677010:intron 55.0%
AACTAGTGCCCTGGATCCCA+GGG - chr4_4:29107085-29107104 None:intergenic 55.0%
TAACTAGTGCCCTGGATCCC+AGG - chr4_4:29107086-29107105 None:intergenic 55.0%
GTGAACAGGGCTGCCTTAAC+AGG + chr4_4:29107228-29107247 Msa0677010:CDS 55.0%
! GCTCTTGGCATGGCTCTGTT+TGG - chr4_4:29107867-29107886 None:intergenic 55.0%
! GCTTTGTTCCGCTCTTGGCA+TGG - chr4_4:29107877-29107896 None:intergenic 55.0%
! CTAGAACCAGAAGGTGGTGG+AGG + chr4_4:29108018-29108037 Msa0677010:CDS 55.0%
GTAGTTCCTCCACCACCTTC+TGG - chr4_4:29108027-29108046 None:intergenic 55.0%
ATCACTTGCGGACCCGGACA+TGG + chr4_4:29106500-29106519 Msa0677010:CDS 60.0%
TTCCTTAACCAGTGCCCCCG+GGG - chr4_4:29106954-29106973 None:intergenic 60.0%
CTTCCTTAACCAGTGCCCCC+GGG - chr4_4:29106955-29106974 None:intergenic 60.0%
GGCTTCCTTAACCAGTGCCC+TGG + chr4_4:29107065-29107084 Msa0677010:intron 60.0%
!! GGATCCCAGGGCACTGGTTA+AGG - chr4_4:29107073-29107092 None:intergenic 60.0%
TAACCAGTGCCCTGGGATCC+AGG + chr4_4:29107073-29107092 Msa0677010:intron 60.0%
AACCAGTGCCCTGGGATCCA+GGG + chr4_4:29107074-29107093 Msa0677010:intron 60.0%
!! GCACGGCTTTGTTCCGCTCT+TGG - chr4_4:29107882-29107901 None:intergenic 60.0%
GCGGACCCGGACATGGAATC+AGG + chr4_4:29106507-29106526 Msa0677010:CDS 65.0%
! TAACCGGTGCCCAGAGGCAC+CGG + chr4_4:29106891-29106910 Msa0677010:intron 65.0%
TAACCGGTGCCTCTGGGCAC+CGG - chr4_4:29106897-29106916 None:intergenic 65.0%
!! TGCCCCGGGGGCACTGGTTA+AGG + chr4_4:29106949-29106968 Msa0677010:intron 70.0%
TGCCCTGGATCCCAGGGCAC+TGG - chr4_4:29107079-29107098 None:intergenic 70.0%
! AACCAGTGCCCCGGGGGCAC+TGG + chr4_4:29106943-29106962 Msa0677010:intron 75.0%
AACCAGTGCCCCCGGGGCAC+TGG - chr4_4:29106948-29106967 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 29104765 29108113 29104765 ID=Msa0677010;Name=Msa0677010
chr4_4 mRNA 29104765 29108113 29104765 ID=Msa0677010-mRNA-1;Parent=Msa0677010;Name=Msa0677010-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0.71|0.5|1|1|1|8|0|444
chr4_4 exon 29104765 29105034 29104765 ID=Msa0677010-mRNA-1:exon:7506;Parent=Msa0677010-mRNA-1
chr4_4 exon 29105154 29105279 29105154 ID=Msa0677010-mRNA-1:exon:7507;Parent=Msa0677010-mRNA-1
chr4_4 exon 29105616 29105786 29105616 ID=Msa0677010-mRNA-1:exon:7508;Parent=Msa0677010-mRNA-1
chr4_4 exon 29106001 29106027 29106001 ID=Msa0677010-mRNA-1:exon:7509;Parent=Msa0677010-mRNA-1
chr4_4 exon 29106118 29106325 29106118 ID=Msa0677010-mRNA-1:exon:7510;Parent=Msa0677010-mRNA-1
chr4_4 exon 29106447 29106528 29106447 ID=Msa0677010-mRNA-1:exon:7511;Parent=Msa0677010-mRNA-1
chr4_4 exon 29107176 29107284 29107176 ID=Msa0677010-mRNA-1:exon:7512;Parent=Msa0677010-mRNA-1
chr4_4 exon 29107772 29108113 29107772 ID=Msa0677010-mRNA-1:exon:7513;Parent=Msa0677010-mRNA-1
chr4_4 CDS 29104765 29105034 29104765 ID=Msa0677010-mRNA-1:cds;Parent=Msa0677010-mRNA-1
chr4_4 CDS 29105154 29105279 29105154 ID=Msa0677010-mRNA-1:cds;Parent=Msa0677010-mRNA-1
chr4_4 CDS 29105616 29105786 29105616 ID=Msa0677010-mRNA-1:cds;Parent=Msa0677010-mRNA-1
chr4_4 CDS 29106001 29106027 29106001 ID=Msa0677010-mRNA-1:cds;Parent=Msa0677010-mRNA-1
chr4_4 CDS 29106118 29106325 29106118 ID=Msa0677010-mRNA-1:cds;Parent=Msa0677010-mRNA-1
chr4_4 CDS 29106447 29106528 29106447 ID=Msa0677010-mRNA-1:cds;Parent=Msa0677010-mRNA-1
chr4_4 CDS 29107176 29107284 29107176 ID=Msa0677010-mRNA-1:cds;Parent=Msa0677010-mRNA-1
chr4_4 CDS 29107772 29108113 29107772 ID=Msa0677010-mRNA-1:cds;Parent=Msa0677010-mRNA-1
Gene Sequence

>Msa0677010

ATGTATCAACAAAAGCCAATTATCACACTTCTCATGATATTCCTAGCTTCATTACTCATATGCAATGGCTCTTACATAGTGGAACCACTTCATCAAGCCAATGTTGAACGTTACTACCATGTACATGATGGTAGATTGATCAAAACAAAACATCATCATTTTGGTCACATAACTAGGACAAATAGAGATACACTTTTCTCTTCAAAGTCTCAAGGAATGGTTAGTGTTGATGACTTTGGAGCAAGAGCAGATGGAAGAGATGATAGAGAGGCATTTAAGAAGGCATGGAATGAAGCATGTTCTAAAGGGTCTATCCTTGTGGTGCCCAAGAATAGGATATACCATCTCAAGCCAATAACTTTTTCAGGTCCATGTCAACCAAATACTGCCTTTATGCTCTATGGAACAATTGAGGCATGGTCTCAAATGTCAGCATATGAAGAAGATAGGCAACACTGGGTTGTGTTTGATAGTGTCTCAAATTTCAAAGTTGATGGTGGTGGCACTTTCAATGGCAATGGGAAAAAATGGTGGCAAAACTCCTGCAAATATTCCAACAATAACCTTCCATGCAATGATGAACCAAGACCAACTGCGGTGACATTCTATGATTGCAAAAATTTGAGAGTAAAGAATCTAAGGTTCAAAGATCCACAACAAATGCATGTAGCATTTGAGAGATGCTTCAATGTGTTTGTTTCGAATTTGATTGTGAGAGCGCCAGAGGATAGTCCCAACACCGATGGAATTCATGTGGCTGAAACACAAAATATTGACATAATAAACTGTGATATTGGGACAGGTGATGATTGTATTTCGATAGTTAGTGGATCCAAAAATGTTCGAGCTATAGATATCACTTGCGGACCCGGACATGGAATCAGTATTGGAAGTTTAGGAGCTGATAACTCAGAAGCTGAAGTTTCCAATGTAGTAGTGAACAGGGCTGCCTTAACAGGAACAACTAATGGAGTGAGAATAAAAACTTGGCAGGGTGGTTCAGGATATGCAAGAAACATCAAATTTATGAACATAGAAATGCAAAATGTGGCAAATCCCATAATCATAGATCAATACTACTGCGACCAAACAGAGCCATGCCAAGAGCGGAACAAAGCCGTGCAATTAAGCCAAGTTTTGTACAAAAACATCAGAGGAACGAGTGCTTCAGAAGTAGCGATCAAATTTAATTGCAGCCGAAGCGTTCCTTGCAGAGAAATATACTTGCAGGATGTGATTCTAGAACCAGAAGGTGGTGGAGGAACTACTGCAACATGTGAGAATGTTAGATATGTAAATAGAGGAAAGTTTTATCCTCAATGCTCTCCACAATAG
Protein sequence

>Msa0677010

MYQQKPIITLLMIFLASLLICNGSYIVEPLHQANVERYYHVHDGRLIKTKHHHFGHITRTNRDTLFSSKSQGMVSVDDFGARADGRDDREAFKKAWNEACSKGSILVVPKNRIYHLKPITFSGPCQPNTAFMLYGTIEAWSQMSAYEEDRQHWVVFDSVSNFKVDGGGTFNGNGKKWWQNSCKYSNNNLPCNDEPRPTAVTFYDCKNLRVKNLRFKDPQQMHVAFERCFNVFVSNLIVRAPEDSPNTDGIHVAETQNIDIINCDIGTGDDCISIVSGSKNVRAIDITCGPGHGISIGSLGADNSEAEVSNVVVNRAALTGTTNGVRIKTWQGGSGYARNIKFMNIEMQNVANPIIIDQYYCDQTEPCQERNKAVQLSQVLYKNIRGTSASEVAIKFNCSRSVPCREIYLQDVILEPEGGGGTTATCENVRYVNRGKFYPQCSPQ*