AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1315480


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1315480 MtrunA17_Chr8g0345171 95.522 134 5 1 1 134 1 133 1.43e-93 266
Msa1315480 MtrunA17_Chr7g0276621 55.469 128 43 6 1 123 23 141 2.06e-37 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1315480 AT3G14280.1 38.889 108 58 3 21 128 21 120 6.14e-15 67.4

Find 29 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTTGAATTGCAATAAAA+TGG 0.221285 8_4:-14956047 Msa1315480:exon
TTCTTGAAGAATTAGCTTAA+TGG 0.238281 8_4:-14957082 Msa1315480:exon
GAATTATCATATTTCTGAAT+TGG 0.275390 8_4:+14957030 None:intergenic
AAGAAGAAGATGAGATAATA+AGG 0.331139 8_4:+14957119 None:intergenic
AATTATCATATTTCTGAATT+GGG 0.345181 8_4:+14957031 None:intergenic
TTGTCTCACTCATAGCTTTG+AGG 0.351441 8_4:-14955994 Msa1315480:three_prime_UTR
AGTTGGTTTCAAATTGCAAA+AGG 0.405294 8_4:-14956117 Msa1315480:CDS
CTTTGGAGCATAGCATCGTT+TGG 0.411384 8_4:+14956975 None:intergenic
TGAATCTTCTAAAACAAAGT+TGG 0.441522 8_4:-14956134 Msa1315480:CDS
CTTGAAATTTGTCTATAAGA+TGG 0.456782 8_4:+14956455 None:intergenic
TCAAATAGCACATGTGTAGT+AGG 0.464126 8_4:-14955925 Msa1315480:three_prime_UTR
TTATTTCTCCACTCTCATCT+TGG 0.480024 8_4:+14956501 None:intergenic
GTTGGTTTCAAATTGCAAAA+GGG 0.500062 8_4:-14956116 Msa1315480:CDS
ATTTGTGAAGGGTATGATGA+AGG 0.501766 8_4:-14956479 Msa1315480:CDS
GACCAACGGTTCATCACAAA+AGG 0.504204 8_4:-14956433 Msa1315480:intron
GAAATTGATGATGCAATTGA+AGG 0.528412 8_4:-14956090 Msa1315480:CDS
GTGGAGAAATAATTTGTGAA+GGG 0.550320 8_4:-14956490 Msa1315480:CDS
AGTGGAGAAATAATTTGTGA+AGG 0.561503 8_4:-14956491 Msa1315480:CDS
TTTGTGAAGGGTATGATGAA+GGG 0.566441 8_4:-14956478 Msa1315480:CDS
CACAGCATCCATCAAGAACA+AGG 0.570428 8_4:-14956917 Msa1315480:intron
ATTCTTCAAGAACAAGAAAG+AGG 0.573168 8_4:+14957093 None:intergenic
AAGAAGATGAGATAATAAGG+AGG 0.575986 8_4:+14957122 None:intergenic
TAGACAAATTTCAAGACCAA+CGG 0.578411 8_4:-14956447 Msa1315480:CDS
TGAAGGTGGTCGTTTGCAAG+AGG 0.580771 8_4:-14956073 Msa1315480:CDS
AAAGTCAACAGTTGGTCCAG+TGG 0.602548 8_4:-14956950 Msa1315480:CDS
CTTTGCTACCAAGATGAGAG+TGG 0.619052 8_4:-14956509 Msa1315480:CDS
CAAAGCTATGAGTGAGACAA+AGG 0.622287 8_4:+14955997 None:intergenic
CTTCAAGAACAAGAAAGAGG+TGG 0.639571 8_4:+14957096 None:intergenic
ATTGATGATGCAATTGAAGG+TGG 0.706142 8_4:-14956087 Msa1315480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATCATATTTCTGAATT+GGG + chr8_4:14955996-14956015 None:intergenic 15.0%
!! AAGAATAGATAAAAGATAAT+AGG + chr8_4:14956140-14956159 None:intergenic 15.0%
!! ATAGATATATGTATATCAAT+TGG + chr8_4:14956183-14956202 None:intergenic 15.0%
!! AAAAAAGATGAAAAAAACTT+TGG - chr8_4:14956229-14956248 Msa1315480:intron 15.0%
!!! AGATAAGTTTTTTCTTTTTT+TGG - chr8_4:14956349-14956368 Msa1315480:intron 15.0%
!!! AGAATAAAATTGTCATTTTA+TGG + chr8_4:14956675-14956694 None:intergenic 15.0%
!!! AAAATGACAATTTTATTCTT+CGG - chr8_4:14956676-14956695 Msa1315480:intron 15.0%
!! TAATTTGAATTGCAATAAAA+TGG - chr8_4:14956977-14956996 Msa1315480:CDS 15.0%
!!! AATTATCATATTTCTGAATT+GGG + chr8_4:14955996-14956015 None:intergenic 15.0%
!! AAGAATAGATAAAAGATAAT+AGG + chr8_4:14956140-14956159 None:intergenic 15.0%
!! ATAGATATATGTATATCAAT+TGG + chr8_4:14956183-14956202 None:intergenic 15.0%
!! AAAAAAGATGAAAAAAACTT+TGG - chr8_4:14956229-14956248 Msa1315480:intron 15.0%
!!! AGATAAGTTTTTTCTTTTTT+TGG - chr8_4:14956349-14956368 Msa1315480:intron 15.0%
!!! AGAATAAAATTGTCATTTTA+TGG + chr8_4:14956675-14956694 None:intergenic 15.0%
!!! AAAATGACAATTTTATTCTT+CGG - chr8_4:14956676-14956695 Msa1315480:intron 15.0%
!! TAATTTGAATTGCAATAAAA+TGG - chr8_4:14956977-14956996 Msa1315480:CDS 15.0%
!!! GAATTATCATATTTCTGAAT+TGG + chr8_4:14955997-14956016 None:intergenic 20.0%
!!! AAAAAAACTTTGGTTAGTTA+TGG - chr8_4:14956239-14956258 Msa1315480:intron 20.0%
!!! TACCTTATAGTTTTTTCTAA+GGG + chr8_4:14956317-14956336 None:intergenic 20.0%
!!! TGGATATATCTACTTTTTAT+TGG - chr8_4:14956369-14956388 Msa1315480:intron 20.0%
!! AATTGATGTTTATATTCTGA+AGG - chr8_4:14956639-14956658 Msa1315480:intron 20.0%
!! ATATAAATATGTCTTACCAA+TGG - chr8_4:14956834-14956853 Msa1315480:intron 20.0%
!!! GAATTATCATATTTCTGAAT+TGG + chr8_4:14955997-14956016 None:intergenic 20.0%
!!! AAAAAAACTTTGGTTAGTTA+TGG - chr8_4:14956239-14956258 Msa1315480:intron 20.0%
!!! TACCTTATAGTTTTTTCTAA+GGG + chr8_4:14956317-14956336 None:intergenic 20.0%
!!! TGGATATATCTACTTTTTAT+TGG - chr8_4:14956369-14956388 Msa1315480:intron 20.0%
!! AATTGATGTTTATATTCTGA+AGG - chr8_4:14956639-14956658 Msa1315480:intron 20.0%
!! ATATAAATATGTCTTACCAA+TGG - chr8_4:14956834-14956853 Msa1315480:intron 20.0%
! AATAAGGAGGAAAAATGAAA+TGG + chr8_4:14955892-14955911 None:intergenic 25.0%
! AAGAAGAAGATGAGATAATA+AGG + chr8_4:14955908-14955927 None:intergenic 25.0%
!! TTCTTGAAGAATTAGCTTAA+TGG - chr8_4:14955942-14955961 Msa1315480:three_prime_UTR 25.0%
!! TTTGGTTAGTTATGGAAATT+TGG - chr8_4:14956247-14956266 Msa1315480:intron 25.0%
! TTTGTCTCTAGTTATATCTT+TGG - chr8_4:14956286-14956305 Msa1315480:intron 25.0%
!!! GTACCTTATAGTTTTTTCTA+AGG + chr8_4:14956318-14956337 None:intergenic 25.0%
! TTTCTCCTAGTAGTAAAAAT+AGG - chr8_4:14956405-14956424 Msa1315480:intron 25.0%
! TGTCTAATTTGATACTAAAC+AGG - chr8_4:14956463-14956482 Msa1315480:CDS 25.0%
!!! TACTAAACAGGTTTTTTATG+AGG - chr8_4:14956475-14956494 Msa1315480:CDS 25.0%
! CTTGAAATTTGTCTATAAGA+TGG + chr8_4:14956572-14956591 None:intergenic 25.0%
!!! TGAATCTTCTAAAACAAAGT+TGG - chr8_4:14956890-14956909 Msa1315480:intron 25.0%
! AATAAGGAGGAAAAATGAAA+TGG + chr8_4:14955892-14955911 None:intergenic 25.0%
! AAGAAGAAGATGAGATAATA+AGG + chr8_4:14955908-14955927 None:intergenic 25.0%
!! TTCTTGAAGAATTAGCTTAA+TGG - chr8_4:14955942-14955961 Msa1315480:three_prime_UTR 25.0%
!! TTTGGTTAGTTATGGAAATT+TGG - chr8_4:14956247-14956266 Msa1315480:intron 25.0%
! TTTGTCTCTAGTTATATCTT+TGG - chr8_4:14956286-14956305 Msa1315480:intron 25.0%
!!! GTACCTTATAGTTTTTTCTA+AGG + chr8_4:14956318-14956337 None:intergenic 25.0%
! TTTCTCCTAGTAGTAAAAAT+AGG - chr8_4:14956405-14956424 Msa1315480:intron 25.0%
! TGTCTAATTTGATACTAAAC+AGG - chr8_4:14956463-14956482 Msa1315480:CDS 25.0%
!!! TACTAAACAGGTTTTTTATG+AGG - chr8_4:14956475-14956494 Msa1315480:CDS 25.0%
! CTTGAAATTTGTCTATAAGA+TGG + chr8_4:14956572-14956591 None:intergenic 25.0%
!!! TGAATCTTCTAAAACAAAGT+TGG - chr8_4:14956890-14956909 Msa1315480:intron 25.0%
AAGAAGATGAGATAATAAGG+AGG + chr8_4:14955905-14955924 None:intergenic 30.0%
ATTCTTCAAGAACAAGAAAG+AGG + chr8_4:14955934-14955953 None:intergenic 30.0%
!! GATTTTAACCTTGTTCTTGA+TGG + chr8_4:14956118-14956137 None:intergenic 30.0%
GTCCCTTAGAAAAAACTATA+AGG - chr8_4:14956312-14956331 Msa1315480:intron 30.0%
ACTATAAGGTACGTCAAAAT+TGG - chr8_4:14956326-14956345 Msa1315480:intron 30.0%
! AGTGGAGAAATAATTTGTGA+AGG - chr8_4:14956533-14956552 Msa1315480:CDS 30.0%
! GTGGAGAAATAATTTGTGAA+GGG - chr8_4:14956534-14956553 Msa1315480:CDS 30.0%
TAGACAAATTTCAAGACCAA+CGG - chr8_4:14956577-14956596 Msa1315480:intron 30.0%
! ATGACAATTTTATTCTTCGG+CGG - chr8_4:14956679-14956698 Msa1315480:intron 30.0%
TGCTACCAGTTAAAATCTTT+CGG - chr8_4:14956707-14956726 Msa1315480:intron 30.0%
AAATGTCGATGAGGTTAATT+TGG - chr8_4:14956735-14956754 Msa1315480:intron 30.0%
!! AGGTTAATTTGGTATTTTCG+TGG - chr8_4:14956746-14956765 Msa1315480:intron 30.0%
! TTTCTAGCATTCTTTGATGT+CGG - chr8_4:14956772-14956791 Msa1315480:intron 30.0%
AGCACAACAAAAAATTCCAT+TGG + chr8_4:14956853-14956872 None:intergenic 30.0%
!!! TGGAATTTTTTGTTGTGCTT+AGG - chr8_4:14956854-14956873 Msa1315480:intron 30.0%
!!! GGAATTTTTTGTTGTGCTTA+GGG - chr8_4:14956855-14956874 Msa1315480:intron 30.0%
! AGTTGGTTTCAAATTGCAAA+AGG - chr8_4:14956907-14956926 Msa1315480:intron 30.0%
! GTTGGTTTCAAATTGCAAAA+GGG - chr8_4:14956908-14956927 Msa1315480:intron 30.0%
!! GAAATTGATGATGCAATTGA+AGG - chr8_4:14956934-14956953 Msa1315480:CDS 30.0%
TAAAATGAAAAATGTGCTGC+AGG + chr8_4:14957131-14957150 None:intergenic 30.0%
AAGAAGATGAGATAATAAGG+AGG + chr8_4:14955905-14955924 None:intergenic 30.0%
ATTCTTCAAGAACAAGAAAG+AGG + chr8_4:14955934-14955953 None:intergenic 30.0%
!! GATTTTAACCTTGTTCTTGA+TGG + chr8_4:14956118-14956137 None:intergenic 30.0%
GTCCCTTAGAAAAAACTATA+AGG - chr8_4:14956312-14956331 Msa1315480:intron 30.0%
ACTATAAGGTACGTCAAAAT+TGG - chr8_4:14956326-14956345 Msa1315480:intron 30.0%
! AGTGGAGAAATAATTTGTGA+AGG - chr8_4:14956533-14956552 Msa1315480:CDS 30.0%
! GTGGAGAAATAATTTGTGAA+GGG - chr8_4:14956534-14956553 Msa1315480:CDS 30.0%
TAGACAAATTTCAAGACCAA+CGG - chr8_4:14956577-14956596 Msa1315480:intron 30.0%
! ATGACAATTTTATTCTTCGG+CGG - chr8_4:14956679-14956698 Msa1315480:intron 30.0%
TGCTACCAGTTAAAATCTTT+CGG - chr8_4:14956707-14956726 Msa1315480:intron 30.0%
AAATGTCGATGAGGTTAATT+TGG - chr8_4:14956735-14956754 Msa1315480:intron 30.0%
!! AGGTTAATTTGGTATTTTCG+TGG - chr8_4:14956746-14956765 Msa1315480:intron 30.0%
! TTTCTAGCATTCTTTGATGT+CGG - chr8_4:14956772-14956791 Msa1315480:intron 30.0%
AGCACAACAAAAAATTCCAT+TGG + chr8_4:14956853-14956872 None:intergenic 30.0%
!!! TGGAATTTTTTGTTGTGCTT+AGG - chr8_4:14956854-14956873 Msa1315480:intron 30.0%
!!! GGAATTTTTTGTTGTGCTTA+GGG - chr8_4:14956855-14956874 Msa1315480:intron 30.0%
! AGTTGGTTTCAAATTGCAAA+AGG - chr8_4:14956907-14956926 Msa1315480:intron 30.0%
! GTTGGTTTCAAATTGCAAAA+GGG - chr8_4:14956908-14956927 Msa1315480:intron 30.0%
!! GAAATTGATGATGCAATTGA+AGG - chr8_4:14956934-14956953 Msa1315480:CDS 30.0%
TAAAATGAAAAATGTGCTGC+AGG + chr8_4:14957131-14957150 None:intergenic 30.0%
!!! CCAACTGTTGACTTTTTCTT+TGG + chr8_4:14956069-14956088 None:intergenic 35.0%
CCAAAGAAAAAGTCAACAGT+TGG - chr8_4:14956066-14956085 Msa1315480:CDS 35.0%
!!! CCTAGCCTATTTTTACTACT+AGG + chr8_4:14956413-14956432 None:intergenic 35.0%
CCTAGTAGTAAAAATAGGCT+AGG - chr8_4:14956410-14956429 Msa1315480:intron 35.0%
! TTTTATGAGGATGAGTCTCA+AGG - chr8_4:14956488-14956507 Msa1315480:CDS 35.0%
! TTATTTCTCCACTCTCATCT+TGG + chr8_4:14956526-14956545 None:intergenic 35.0%
! ATTTGTGAAGGGTATGATGA+AGG - chr8_4:14956545-14956564 Msa1315480:intron 35.0%
! TTTGTGAAGGGTATGATGAA+GGG - chr8_4:14956546-14956565 Msa1315480:intron 35.0%
TACCAGTTAAAATCTTTCGG+CGG - chr8_4:14956710-14956729 Msa1315480:intron 35.0%
!! ATTGATGATGCAATTGAAGG+TGG - chr8_4:14956937-14956956 Msa1315480:CDS 35.0%
TCAAATAGCACATGTGTAGT+AGG - chr8_4:14957099-14957118 Msa1315480:five_prime_UTR 35.0%
!!! CCAACTGTTGACTTTTTCTT+TGG + chr8_4:14956069-14956088 None:intergenic 35.0%
CCAAAGAAAAAGTCAACAGT+TGG - chr8_4:14956066-14956085 Msa1315480:CDS 35.0%
!!! CCTAGCCTATTTTTACTACT+AGG + chr8_4:14956413-14956432 None:intergenic 35.0%
CCTAGTAGTAAAAATAGGCT+AGG - chr8_4:14956410-14956429 Msa1315480:intron 35.0%
! TTTTATGAGGATGAGTCTCA+AGG - chr8_4:14956488-14956507 Msa1315480:CDS 35.0%
! TTATTTCTCCACTCTCATCT+TGG + chr8_4:14956526-14956545 None:intergenic 35.0%
! ATTTGTGAAGGGTATGATGA+AGG - chr8_4:14956545-14956564 Msa1315480:intron 35.0%
! TTTGTGAAGGGTATGATGAA+GGG - chr8_4:14956546-14956565 Msa1315480:intron 35.0%
TACCAGTTAAAATCTTTCGG+CGG - chr8_4:14956710-14956729 Msa1315480:intron 35.0%
!! ATTGATGATGCAATTGAAGG+TGG - chr8_4:14956937-14956956 Msa1315480:CDS 35.0%
TCAAATAGCACATGTGTAGT+AGG - chr8_4:14957099-14957118 Msa1315480:five_prime_UTR 35.0%
CTTCAAGAACAAGAAAGAGG+TGG + chr8_4:14955931-14955950 None:intergenic 40.0%
! GCTGTGATTTTCAATACCAC+TGG + chr8_4:14956093-14956112 None:intergenic 40.0%
! TACCTTTTGTGATGAACCGT+TGG + chr8_4:14956596-14956615 None:intergenic 40.0%
!! AACCGCCGAAAGATTTTAAC+TGG + chr8_4:14956715-14956734 None:intergenic 40.0%
CAAAGCTATGAGTGAGACAA+AGG + chr8_4:14957030-14957049 None:intergenic 40.0%
! TTGTCTCACTCATAGCTTTG+AGG - chr8_4:14957030-14957049 Msa1315480:CDS 40.0%
CTTCAAGAACAAGAAAGAGG+TGG + chr8_4:14955931-14955950 None:intergenic 40.0%
! GCTGTGATTTTCAATACCAC+TGG + chr8_4:14956093-14956112 None:intergenic 40.0%
! TACCTTTTGTGATGAACCGT+TGG + chr8_4:14956596-14956615 None:intergenic 40.0%
!! AACCGCCGAAAGATTTTAAC+TGG + chr8_4:14956715-14956734 None:intergenic 40.0%
CAAAGCTATGAGTGAGACAA+AGG + chr8_4:14957030-14957049 None:intergenic 40.0%
! TTGTCTCACTCATAGCTTTG+AGG - chr8_4:14957030-14957049 Msa1315480:CDS 40.0%
!! CTTTGGAGCATAGCATCGTT+TGG + chr8_4:14956052-14956071 None:intergenic 45.0%
AAAGTCAACAGTTGGTCCAG+TGG - chr8_4:14956074-14956093 Msa1315480:CDS 45.0%
CACAGCATCCATCAAGAACA+AGG - chr8_4:14956107-14956126 Msa1315480:CDS 45.0%
CTTTGCTACCAAGATGAGAG+TGG - chr8_4:14956515-14956534 Msa1315480:CDS 45.0%
GACCAACGGTTCATCACAAA+AGG - chr8_4:14956591-14956610 Msa1315480:intron 45.0%
!! CTTTGGAGCATAGCATCGTT+TGG + chr8_4:14956052-14956071 None:intergenic 45.0%
AAAGTCAACAGTTGGTCCAG+TGG - chr8_4:14956074-14956093 Msa1315480:CDS 45.0%
CACAGCATCCATCAAGAACA+AGG - chr8_4:14956107-14956126 Msa1315480:CDS 45.0%
CTTTGCTACCAAGATGAGAG+TGG - chr8_4:14956515-14956534 Msa1315480:CDS 45.0%
GACCAACGGTTCATCACAAA+AGG - chr8_4:14956591-14956610 Msa1315480:intron 45.0%
TCGGCGGTTAAATGTCGATG+AGG - chr8_4:14956726-14956745 Msa1315480:intron 50.0%
TGAAGGTGGTCGTTTGCAAG+AGG - chr8_4:14956951-14956970 Msa1315480:CDS 50.0%
TCGGCGGTTAAATGTCGATG+AGG - chr8_4:14956726-14956745 Msa1315480:intron 50.0%
TGAAGGTGGTCGTTTGCAAG+AGG - chr8_4:14956951-14956970 Msa1315480:CDS 50.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 14955888 14957158 14955888 ID=Msa1315480;Name=Msa1315480
chr8_4 mRNA 14955888 14957158 14955888 ID=Msa1315480-mRNA-1;Parent=Msa1315480;Name=Msa1315480-mRNA-1;_AED=0.06;_eAED=0.06;_QI=73|1|1|1|1|1|3|174|134
chr8_4 exon 14955888 14956170 14955888 ID=Msa1315480-mRNA-1:exon:5684;Parent=Msa1315480-mRNA-1
chr8_4 exon 14956434 14956561 14956434 ID=Msa1315480-mRNA-1:exon:5683;Parent=Msa1315480-mRNA-1
chr8_4 exon 14956918 14957158 14956918 ID=Msa1315480-mRNA-1:exon:5682;Parent=Msa1315480-mRNA-1
chr8_4 five_prime_UTR 14957086 14957158 14957086 ID=Msa1315480-mRNA-1:five_prime_utr;Parent=Msa1315480-mRNA-1
chr8_4 CDS 14956918 14957085 14956918 ID=Msa1315480-mRNA-1:cds;Parent=Msa1315480-mRNA-1
chr8_4 CDS 14956434 14956561 14956434 ID=Msa1315480-mRNA-1:cds;Parent=Msa1315480-mRNA-1
chr8_4 CDS 14956062 14956170 14956062 ID=Msa1315480-mRNA-1:cds;Parent=Msa1315480-mRNA-1
chr8_4 three_prime_UTR 14955888 14956061 14955888 ID=Msa1315480-mRNA-1:three_prime_utr;Parent=Msa1315480-mRNA-1
Gene Sequence

>Msa1315480

ATGGCTGTTCACTGCAATTCTTTAGCTCAAATCCCAATTCAGAAATATGATAATTCATTTGTGAAATATTCAAACAATTTGTTGATGTCCAAACGATGCTATGCTCCAAAGAAAAAGTCAACAGTTGGTCCAGTGGTATTGAAAATCACAGCATCCATCAAGAACAAGGTTTTTTATGAGGATGAGTCTCAAGGTATACTTTGCTACCAAGATGAGAGTGGAGAAATAATTTGTGAAGGGTATGATGAAGGGCCATCTTATAGACAAATTTCAAGACCAACGGTTCATCACAAAAGGGATGTAGAAATTGTGAATCTTCTAAAACAAAGTTGGTTTCAAATTGCAAAAGGGAAAGAAATTGATGATGCAATTGAAGGTGGTCGTTTGCAAGAGGATTTAATTTGA

Protein sequence

>Msa1315480

MAVHCNSLAQIPIQKYDNSFVKYSNNLLMSKRCYAPKKKSTVGPVVLKITASIKNKVFYEDESQGILCYQDESGEIICEGYDEGPSYRQISRPTVHHKRDVEIVNLLKQSWFQIAKGKEIDDAIEGGRLQEDLI*