AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0598060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0598060 MtrunA17_Chr4g0020771 95.495 444 20 0 1 444 1 444 0.0 896
Msa0598060 MtrunA17_Chr3g0089021 60.000 445 168 6 1 443 2 438 0.0 523
Msa0598060 MtrunA17_Chr1g0173451 50.455 440 199 6 8 441 9 435 9.67e-145 421
Msa0598060 MtrunA17_Chr2g0332801 46.092 371 193 5 73 439 58 425 6.10e-113 339
Msa0598060 MtrunA17_Chr8g0365901 38.914 442 243 9 7 439 6 429 1.33e-108 328
Msa0598060 MtrunA17_Chr7g0257701 47.368 342 132 3 62 365 53 384 1.61e-101 308
Msa0598060 MtrunA17_Chr7g0257721 49.390 328 151 6 11 330 11 331 7.06e-96 291
Msa0598060 MtrunA17_Chr6g0449961 44.023 343 179 7 76 415 47 379 5.47e-93 287
Msa0598060 MtrunA17_Chr4g0052621 43.251 363 191 7 68 419 59 417 4.33e-90 281
Msa0598060 MtrunA17_Chr7g0270361 40.872 367 207 6 62 419 55 420 8.40e-90 280
Msa0598060 MtrunA17_Chr3g0121881 40.108 369 202 7 70 430 29 386 7.30e-87 270
Msa0598060 MtrunA17_Chr5g0413141 38.043 368 219 7 79 439 64 429 1.63e-86 271
Msa0598060 MtrunA17_Chr2g0303941 41.623 382 205 10 65 440 19 388 1.69e-86 269
Msa0598060 MtrunA17_Chr1g0194231 40.872 367 192 9 70 419 77 435 5.49e-86 271
Msa0598060 MtrunA17_Chr4g0043661 38.133 375 204 8 71 430 20 381 2.06e-84 264
Msa0598060 MtrunA17_Chr8g0365891 38.360 378 196 7 70 416 103 474 3.59e-84 267
Msa0598060 MtrunA17_Chr5g0418411 41.111 360 194 8 79 430 34 383 1.85e-83 262
Msa0598060 MtrunA17_Chr3g0121891 39.776 357 194 7 79 426 34 378 6.32e-82 258
Msa0598060 MtrunA17_Chr2g0332971 40.525 343 196 5 76 412 30 370 3.01e-80 254
Msa0598060 MtrunA17_Chr5g0413411 38.275 371 193 8 76 416 123 487 9.51e-80 256
Msa0598060 MtrunA17_Chr7g0230891 39.353 371 207 10 76 440 39 397 3.17e-77 246
Msa0598060 MtrunA17_Chr4g0067631 37.469 403 230 14 51 443 32 422 6.59e-77 246
Msa0598060 MtrunA17_Chr8g0348981 40.000 355 196 9 73 418 26 372 9.94e-75 239
Msa0598060 MtrunA17_Chr8g0337571 38.874 373 200 9 70 434 27 379 9.43e-74 236
Msa0598060 MtrunA17_Chr8g0337581 38.378 370 201 9 76 437 33 383 3.40e-72 232
Msa0598060 MtrunA17_Chr4g0043431 40.116 344 193 9 76 414 37 372 1.08e-71 231
Msa0598060 MtrunA17_Chr6g0461181 35.149 404 205 9 75 432 31 423 1.41e-71 232
Msa0598060 MtrunA17_Chr7g0228411 36.074 377 206 10 80 428 46 415 4.59e-70 228
Msa0598060 MtrunA17_Chr2g0293681 40.122 329 183 6 68 386 61 385 8.12e-70 227
Msa0598060 MtrunA17_Chr4g0018151 33.705 448 247 10 12 430 1 427 1.30e-69 227
Msa0598060 MtrunA17_Chr2g0307831 35.171 381 236 9 66 441 17 391 7.20e-69 224
Msa0598060 MtrunA17_Chr2g0307821 35.171 381 236 9 66 441 17 391 7.20e-69 224
Msa0598060 MtrunA17_Chr3g0100091 38.889 288 160 5 142 428 59 331 1.00e-66 217
Msa0598060 MtrunA17_Chr2g0291841 37.758 339 201 8 104 440 29 359 1.57e-65 214
Msa0598060 MtrunA17_Chr2g0307811 36.316 380 219 9 73 441 24 391 4.39e-65 214
Msa0598060 MtrunA17_Chr1g0195221 33.165 395 242 11 57 439 18 402 7.70e-64 211
Msa0598060 MtrunA17_Chr2g0295451 36.544 353 189 13 62 393 43 381 7.52e-61 202
Msa0598060 MtrunA17_Chr1g0150291 34.783 345 207 9 74 412 45 377 2.11e-60 202
Msa0598060 MtrunA17_Chr1g0150301 35.072 345 206 9 74 412 27 359 2.00e-59 199
Msa0598060 MtrunA17_Chr5g0425811 37.102 283 161 7 90 367 2 272 4.91e-56 187
Msa0598060 MtrunA17_Chr8g0336481 38.095 273 151 4 131 401 14 270 2.11e-54 182
Msa0598060 MtrunA17_Chr5g0447901 35.759 316 186 9 73 382 24 328 1.07e-52 180
Msa0598060 MtrunA17_Chr3g0093011 41.964 224 127 1 199 419 13 236 2.28e-52 176
Msa0598060 MtrunA17_Chr8g0337601 43.038 237 128 4 202 437 4 234 3.12e-52 176
Msa0598060 MtrunA17_Chr8g0337511 40.260 231 130 3 199 428 4 227 1.31e-51 174
Msa0598060 MtrunA17_Chr8g0337521 36.170 282 155 7 54 329 11 273 4.70e-47 164
Msa0598060 MtrunA17_Chr7g0273191 52.143 140 64 2 174 313 2 138 2.20e-44 152
Msa0598060 MtrunA17_Chr8g0337351 46.667 165 87 1 217 380 8 172 4.53e-42 147
Msa0598060 MtrunA17_Chr1g0149241 29.919 371 212 9 66 430 20 348 5.78e-42 152
Msa0598060 MtrunA17_Chr8g0337551 48.438 128 66 0 202 329 7 134 5.88e-31 116
Msa0598060 MtrunA17_Chr7g0257711 50.862 116 56 1 330 444 1 116 1.38e-30 114
Msa0598060 MtrunA17_Chr4g0067811 24.198 405 230 11 74 429 40 416 1.26e-21 97.1
Msa0598060 MtrunA17_Chr2g0301091 28.958 259 159 7 168 412 169 416 1.66e-20 93.6
Msa0598060 MtrunA17_Chr3g0095221 25.581 387 230 14 74 421 47 414 3.27e-20 92.8
Msa0598060 MtrunA17_Chr5g0442221 27.273 264 169 6 168 419 158 410 1.69e-18 87.4
Msa0598060 MtrunA17_Chr7g0273181 47.500 80 42 0 347 426 1 80 2.68e-18 80.9
Msa0598060 MtrunA17_Chr7g0270821 26.158 367 201 13 74 394 43 385 2.89e-18 87.0
Msa0598060 MtrunA17_Chr1g0193961 26.891 357 194 13 74 386 43 376 3.33e-18 86.7
Msa0598060 MtrunA17_Chr4g0070281 26.255 259 166 7 168 412 188 435 3.43e-18 86.7
Msa0598060 MtrunA17_Chr7g0255311 23.325 403 249 15 54 415 43 426 1.17e-16 82.0
Msa0598060 MtrunA17_Chr3g0093021 41.221 131 64 9 68 190 17 142 1.76e-16 77.0
Msa0598060 MtrunA17_Chr2g0295241 21.975 405 239 10 74 429 37 413 2.68e-16 80.9
Msa0598060 MtrunA17_Chr2g0330221 31.522 184 116 6 67 247 17 193 4.75e-16 76.6
Msa0598060 MtrunA17_Chr3g0130251 25.794 252 163 6 157 394 177 418 7.18e-16 79.7
Msa0598060 MtrunA17_Chr8g0348491 24.432 352 218 13 74 390 55 393 1.33e-15 79.0
Msa0598060 MtrunA17_Chr8g0387391 25.633 316 177 11 75 350 45 342 1.66e-15 78.6
Msa0598060 MtrunA17_Chr7g0248451 22.816 412 250 13 75 441 56 444 2.08e-14 75.1
Msa0598060 MtrunA17_Chr1g0202651 22.751 378 237 11 76 415 57 417 2.69e-14 74.7
Msa0598060 MtrunA17_Chr8g0373171 41.772 79 46 0 200 278 7 85 6.05e-14 68.2
Msa0598060 MtrunA17_Chr8g0337541 32.812 128 73 6 58 179 20 140 8.20e-14 68.9
Msa0598060 MtrunA17_Chr1g0202641 22.487 378 238 12 76 415 57 417 9.51e-13 70.5
Msa0598060 MtrunA17_Chr1g0202641 22.372 371 247 13 76 415 557 917 1.15e-11 67.0
Msa0598060 MtrunA17_Chr7g0258111 24.815 270 177 5 147 400 123 382 1.72e-12 69.3
Msa0598060 MtrunA17_Chr8g0337561 39.423 104 56 3 332 434 8 105 4.64e-12 62.8
Msa0598060 MtrunA17_Chr8g0337531 38.679 106 58 3 330 434 23 122 3.22e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0598060 AT3G07970.1 52.643 454 188 11 1 442 1 439 4.98e-151 436
Msa0598060 AT3G57510.1 48.416 442 200 12 11 442 8 431 8.04e-138 402
Msa0598060 AT2G41850.1 49.433 441 197 12 10 441 7 430 1.18e-126 374
Msa0598060 AT1G80170.1 47.606 376 189 6 69 439 48 420 2.91e-117 350
Msa0598060 AT1G23460.2 45.119 379 199 7 66 439 33 407 3.25e-108 327
Msa0598060 AT1G23460.1 45.119 379 199 7 66 439 57 431 5.72e-108 327
Msa0598060 AT1G70500.1 43.536 379 205 7 66 439 57 431 2.20e-104 318
Msa0598060 AT1G56710.1 43.978 357 183 7 76 419 50 402 8.48e-93 287
Msa0598060 AT3G15720.3 42.703 370 190 8 70 430 19 375 3.12e-92 286
Msa0598060 AT3G15720.1 42.703 370 190 8 70 430 20 376 3.21e-92 286
Msa0598060 AT5G14650.1 40.984 366 201 7 59 419 36 391 1.47e-90 281
Msa0598060 AT3G15720.2 42.432 370 188 9 70 430 19 372 2.64e-89 279
Msa0598060 AT1G48100.1 40.054 367 197 8 68 419 74 432 1.98e-87 275
Msa0598060 AT4G35670.1 41.389 360 194 7 76 429 26 374 1.23e-86 270
Msa0598060 AT2G43890.1 39.257 377 217 8 67 440 22 389 2.86e-86 269
Msa0598060 AT3G26610.1 35.714 420 250 9 6 419 8 413 3.82e-85 269
Msa0598060 AT5G27530.1 39.944 358 190 5 79 430 58 396 5.44e-85 268
Msa0598060 AT1G05650.1 39.362 376 217 7 67 439 21 388 1.92e-83 262
Msa0598060 AT3G15720.4 43.034 323 165 6 114 430 3 312 6.24e-83 260
Msa0598060 AT1G02460.1 41.033 368 189 9 72 419 87 446 1.38e-82 263
Msa0598060 AT1G05660.1 38.992 377 217 8 67 439 21 388 1.07e-81 257
Msa0598060 AT1G60590.1 38.378 370 197 7 76 416 125 492 1.38e-81 261
Msa0598060 AT1G80140.1 41.195 318 169 7 108 419 6 311 2.28e-80 252
Msa0598060 AT1G10640.1 37.875 367 200 7 76 416 121 485 6.26e-80 257
Msa0598060 AT5G44840.1 37.778 360 208 7 76 429 26 375 2.78e-78 248
Msa0598060 AT4G18180.1 37.566 378 210 8 73 430 38 409 3.37e-78 249
Msa0598060 AT1G80170.2 46.816 267 135 5 69 331 48 311 2.07e-77 244
Msa0598060 AT2G43870.1 39.426 383 215 12 65 441 12 383 1.16e-76 244
Msa0598060 AT5G17200.1 39.018 387 195 9 70 429 35 407 2.42e-76 244
Msa0598060 AT2G33160.1 39.096 376 213 9 73 439 31 399 5.22e-76 250
Msa0598060 AT4G01890.1 38.268 358 201 8 76 417 67 420 1.58e-75 244
Msa0598060 AT3G59850.1 40.351 342 189 10 79 414 28 360 5.83e-75 240
Msa0598060 AT3G59850.2 40.233 343 190 10 78 414 71 404 1.04e-74 240
Msa0598060 AT2G15460.1 35.884 379 224 9 73 440 30 400 2.39e-74 239
Msa0598060 AT1G43080.1 37.368 380 217 11 73 440 30 400 3.09e-74 238
Msa0598060 AT1G02790.1 37.598 383 206 11 76 441 54 420 4.10e-74 239
Msa0598060 AT2G15450.1 35.714 378 224 9 73 439 30 399 1.19e-73 237
Msa0598060 AT2G15470.1 35.620 379 225 9 73 440 30 400 5.19e-73 235
Msa0598060 AT1G43090.1 36.800 375 218 9 73 436 30 396 2.06e-72 235
Msa0598060 AT2G26620.1 36.340 377 223 9 73 440 30 398 4.73e-72 233
Msa0598060 AT2G43880.1 38.810 353 201 8 67 414 24 366 1.84e-71 231
Msa0598060 AT4G35670.2 42.599 277 148 2 153 429 2 267 2.24e-71 227
Msa0598060 AT2G40310.1 36.340 377 219 11 76 440 33 400 2.67e-71 231
Msa0598060 AT1G17150.1 37.466 371 217 9 76 439 35 397 3.51e-71 230
Msa0598060 AT3G07820.1 36.585 369 218 9 81 442 28 387 3.85e-71 230
Msa0598060 AT1G43100.1 36.533 375 219 9 73 436 30 396 3.97e-71 231
Msa0598060 AT1G65570.1 35.162 401 234 10 46 440 14 394 5.06e-71 230
Msa0598060 AT5G48140.1 37.892 351 202 8 85 429 33 373 1.39e-70 229
Msa0598060 AT4G13760.1 35.092 379 227 9 73 440 30 400 1.58e-70 229
Msa0598060 AT1G78400.1 36.828 372 218 11 76 439 37 399 1.80e-70 229
Msa0598060 AT2G43860.1 39.276 359 202 10 64 414 20 370 2.47e-70 228
Msa0598060 AT3G07840.1 35.849 371 215 9 73 429 13 374 7.70e-70 227
Msa0598060 AT3G07830.1 35.635 362 218 8 85 440 33 385 1.29e-69 226
Msa0598060 AT3G14040.1 36.997 373 219 9 74 439 71 434 1.47e-67 222
Msa0598060 AT5G44830.1 36.810 326 184 4 106 429 11 316 1.69e-67 219
Msa0598060 AT4G32380.1 34.946 372 222 8 73 434 25 386 4.10e-67 221
Msa0598060 AT3G07850.1 36.675 379 224 9 68 439 64 433 4.54e-67 221
Msa0598060 AT5G14650.2 38.849 278 157 5 59 331 36 305 1.06e-61 203
Msa0598060 AT4G32370.1 37.540 309 171 7 69 365 29 327 8.75e-61 201
Msa0598060 AT2G43880.2 38.511 309 175 8 67 370 24 322 3.41e-58 194
Msa0598060 AT4G32375.1 39.080 261 148 3 153 412 13 263 4.11e-58 197
Msa0598060 AT4G32380.2 31.081 370 198 8 73 434 25 345 4.78e-52 181
Msa0598060 AT5G39910.1 34.754 305 168 7 73 369 35 316 5.13e-52 179
Msa0598060 AT1G19170.1 28.185 259 161 6 168 412 201 448 7.03e-21 95.5
Msa0598060 AT3G62110.1 28.879 232 142 5 168 386 156 377 1.38e-20 94.4
Msa0598060 AT3G62110.2 28.879 232 142 5 168 386 156 377 1.38e-20 94.4
Msa0598060 AT3G42950.1 26.738 374 228 14 73 412 65 426 3.33e-20 93.2
Msa0598060 AT4G23820.1 24.810 395 239 14 74 429 41 416 7.96e-20 92.0
Msa0598060 AT4G33440.1 25.000 392 218 14 73 408 71 442 9.83e-18 85.9
Msa0598060 AT2G23900.1 25.316 316 177 10 76 350 58 355 2.01e-17 84.7
Msa0598060 AT3G16850.1 23.774 265 170 5 168 415 153 402 2.79e-17 84.3
Msa0598060 AT5G41870.1 25.141 354 207 14 73 386 44 379 2.95e-17 84.0
Msa0598060 AT3G06770.5 24.147 381 226 12 74 412 44 403 3.87e-17 84.0
Msa0598060 AT3G06770.2 24.147 381 226 12 74 412 43 402 4.41e-17 83.6
Msa0598060 AT3G48950.1 24.207 347 198 11 47 350 20 344 4.90e-17 83.6
Msa0598060 AT5G49215.2 23.438 384 231 11 74 415 41 403 2.09e-16 81.6
Msa0598060 AT5G49215.1 23.438 384 231 11 74 415 43 405 2.23e-16 81.6
Msa0598060 AT4G23500.1 25.316 316 178 11 75 350 69 366 1.03e-15 79.7
Msa0598060 AT3G06770.1 24.031 258 172 5 168 412 87 333 4.51e-15 77.0
Msa0598060 AT3G06770.3 24.031 258 172 5 168 412 87 333 4.51e-15 77.0
Msa0598060 AT3G06770.4 24.031 258 172 5 168 412 69 315 5.84e-15 76.6
Msa0598060 AT3G61490.4 30.729 192 112 6 168 346 271 454 2.04e-14 75.9
Msa0598060 AT3G61490.5 30.729 192 112 6 168 346 173 356 2.20e-14 75.5
Msa0598060 AT3G61490.2 30.890 191 111 6 168 345 158 340 2.27e-14 75.5
Msa0598060 AT3G61490.3 30.890 191 111 6 168 345 158 340 2.27e-14 75.5
Msa0598060 AT3G61490.1 30.890 191 111 6 168 345 158 340 2.27e-14 75.5
Msa0598060 AT3G57790.2 25.369 339 213 11 74 384 35 361 4.16e-11 65.1
Msa0598060 AT3G57790.1 25.369 339 213 11 74 384 35 361 4.16e-11 65.1

Find 88 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACCAAATACTGCCTTTA+TGG 0.149151 4_2:+32027081 Msa0598060:CDS
GACATAATAAACTGTGATAT+TGG 0.217261 4_2:+32028122 Msa0598060:CDS
CTCACCATAAAGGCAGTATT+TGG 0.245870 4_2:-32027085 None:intergenic
AAAACAAAACATCATCATTT+TGG 0.257008 4_2:+32026729 Msa0598060:CDS
AATTCATCATGTTAGCTCTA+TGG 0.292346 4_2:+32027423 Msa0598060:intron
GTAGTTCCTCCACCACCTTC+TGG 0.295016 4_2:-32029781 None:intergenic
TTCTGCAGTATTGGAAGTTT+AGG 0.326431 4_2:+32028916 Msa0598060:intron
GTTATTGTTGGAATATTTGC+AGG 0.329378 4_2:-32027583 None:intergenic
ATTTGAGAGTAAAGAATCTA+AGG 0.331559 4_2:+32027967 Msa0598060:CDS
GAGATGGTATATCCTATTCT+TGG 0.332828 4_2:-32027032 None:intergenic
TTGCAGAGAAATATACTTGC+AGG 0.334080 4_2:+32029744 Msa0598060:CDS
GCACGGCTTTGTTCCGCTCT+TGG 0.338883 4_2:-32029636 None:intergenic
GTAGTATTGATCTATGATTA+TGG 0.353266 4_2:-32029593 None:intergenic
ATCATGAGAAGTGTGATAAT+TGG 0.354858 4_2:-32026603 None:intergenic
ATATGATCGCAGGGTGGTTC+TGG 0.365600 4_2:+32029517 Msa0598060:intron
GTCTCAAATTTCAAAGTTGA+TGG 0.380238 4_2:+32027516 Msa0598060:CDS
GCTCTTGGCATGGCTCTGTT+TGG 0.383793 4_2:-32029621 None:intergenic
GCGGACCCGGACATGGAATC+AGG 0.384459 4_2:+32028332 Msa0598060:CDS
AAAACTTACAAGGTTATTGT+TGG 0.389616 4_2:-32027595 None:intergenic
ACATAATAAACTGTGATATT+GGG 0.395546 4_2:+32028123 Msa0598060:CDS
TTTCCAATGTAGTAGTGAAC+AGG 0.405039 4_2:+32028962 Msa0598060:CDS
CTCCTTTAAGGCATTTAAGA+AGG 0.417702 4_2:+32026967 Msa0598060:intron
GTGAACAGGGCTACATTAAC+AGG 0.418572 4_2:+32028976 Msa0598060:CDS
ATGAAACCTGATTCCATGTC+CGG 0.431003 4_2:-32028338 None:intergenic
TGTCAGCATATGAAGAAGAT+AGG 0.435363 4_2:+32027469 Msa0598060:CDS
TATGATCTTACAGTTGGTCT+TGG 0.435649 4_2:-32027840 None:intergenic
GATTGTATTTCGATAGTTAG+TGG 0.441436 4_2:+32028277 Msa0598060:CDS
ACATTAACAGGAACAACTAA+TGG 0.445131 4_2:+32028988 Msa0598060:CDS
TAGTATTGATCTATGATTAT+GGG 0.445220 4_2:-32029592 None:intergenic
TCCTCAATGCTCTCCACAAT+AGG 0.452988 4_2:+32029849 Msa0598060:CDS
GATGGTGGTGGCACTTTCAA+TGG 0.457262 4_2:+32027534 Msa0598060:CDS
AGATGGTATATCCTATTCTT+GGG 0.459795 4_2:-32027031 None:intergenic
TAGTGTAAGACTCACCATAA+AGG 0.459802 4_2:-32027095 None:intergenic
AGCCCTGTTCACTACTACAT+TGG 0.464076 4_2:-32028965 None:intergenic
CACATGAATTCCATCGGTGT+TGG 0.469737 4_2:-32028081 None:intergenic
GCTTTGTTCCGCTCTTGGCA+TGG 0.478055 4_2:-32029631 None:intergenic
CAAAACTTGGCTTAATTGCA+CGG 0.479353 4_2:-32029653 None:intergenic
ACATGAATTCCATCGGTGTT+GGG 0.481545 4_2:-32028080 None:intergenic
ACAATATCCATTGCCTATTG+TGG 0.484295 4_2:-32029862 None:intergenic
TGGAATGAAGCATGTTCTAA+AGG 0.492280 4_2:+32026992 Msa0598060:CDS
CTCTTCAAAGTCTCAAGGAA+TGG 0.495111 4_2:+32026785 Msa0598060:CDS
GGAATGAAGCATGTTCTAAA+GGG 0.497809 4_2:+32026993 Msa0598060:CDS
ATGGTTAGTGTTGATGACTT+TGG 0.500293 4_2:+32026804 Msa0598060:CDS
AGATGGAAGAGATGATAGAG+AGG 0.500756 4_2:+32026836 Msa0598060:CDS
GACTTTGGAGCAAGAGCAGA+TGG 0.502754 4_2:+32026819 Msa0598060:CDS
TCCTTGTGGTGCCCAAGAAT+AGG 0.505871 4_2:+32027020 Msa0598060:CDS
TCGGTGTTGGGACTATCCTC+TGG 0.506883 4_2:-32028068 None:intergenic
AATTTCAAAGTTGATGGTGG+TGG 0.508006 4_2:+32027522 Msa0598060:CDS
TTTCTCTGCAAGGAACGCTT+CGG 0.508888 4_2:-32029732 None:intergenic
TTAAGGCATTTAAGAAGGCA+TGG 0.511868 4_2:+32026972 Msa0598060:intron
ATAGATATCACTTGCGGACC+CGG 0.513667 4_2:+32028319 Msa0598060:CDS
TCAACATTGGCTTGATGAAG+TGG 0.522002 4_2:-32026672 None:intergenic
GTGGCACTTTCAATGGCAAT+GGG 0.535626 4_2:+32027541 Msa0598060:CDS
ATCACTTGCGGACCCGGACA+TGG 0.542140 4_2:+32028325 Msa0598060:CDS
GGTGGCACTTTCAATGGCAA+TGG 0.545089 4_2:+32027540 Msa0598060:CDS
GGTCTTGGTTCATCATTGCA+TGG 0.546282 4_2:-32027825 None:intergenic
ATGCAATGGCTCTTACATAG+TGG 0.549275 4_2:+32026647 Msa0598060:CDS
ATGAAGAAGATAGGCAACAC+TGG 0.549453 4_2:+32027478 Msa0598060:CDS
ATAAACTGTGATATTGGGAC+AGG 0.552284 4_2:+32028128 Msa0598060:CDS
TCAATCTACCATCATGTACA+TGG 0.568484 4_2:-32026704 None:intergenic
AATGTTAGATATGTAAATAG+AGG 0.571277 4_2:+32029817 Msa0598060:CDS
TTCAGCCACATGAATTCCAT+CGG 0.571554 4_2:-32028087 None:intergenic
TTCTAAAGGGTCTATCCTTG+TGG 0.572564 4_2:+32027006 Msa0598060:CDS
TGAAACCTGATTCCATGTCC+GGG 0.576277 4_2:-32028337 None:intergenic
CTAGAACCAGAAGGTGGTGG+AGG 0.582001 4_2:+32029775 Msa0598060:CDS
GCCTATTGTGGAGAGCATTG+AGG 0.588158 4_2:-32029850 None:intergenic
TGCAAGTATATTTCTCTGCA+AGG 0.588447 4_2:-32029742 None:intergenic
GAACATAGAAATGCAAAATG+TGG 0.591101 4_2:+32029564 Msa0598060:CDS
ATTCTAGAACCAGAAGGTGG+TGG 0.593437 4_2:+32029772 Msa0598060:CDS
AAGGCAGTATTTGGTTGACA+TGG 0.594353 4_2:-32027076 None:intergenic
AATGCTACATGCATTTGTTG+TGG 0.595049 4_2:-32027999 None:intergenic
TGCATATGAGTAATGAAGCT+AGG 0.596335 4_2:-32026629 None:intergenic
GAGGATAGTCCCAACACCGA+TGG 0.596952 4_2:+32028071 Msa0598060:CDS
CGTTACTACCATGTACATGA+TGG 0.600317 4_2:+32026696 Msa0598060:CDS
TCCTATTCTTGGGCACCACA+AGG 0.600648 4_2:-32027021 None:intergenic
TTAGCTCTATGGAACAATTG+AGG 0.602214 4_2:+32027434 Msa0598060:intron
ATGGTAGTAACGTTCAACAT+TGG 0.605356 4_2:-32026685 None:intergenic
TTTGATTGTGAGAGCGCCAG+AGG 0.607314 4_2:+32028052 Msa0598060:CDS
AAACAGAGCCATGCCAAGAG+CGG 0.620744 4_2:+32029623 Msa0598060:CDS
GCTTCATTACTCATATGCAA+TGG 0.623445 4_2:+32026633 Msa0598060:CDS
TCAAATTTCAAAGTTGATGG+TGG 0.632469 4_2:+32027519 Msa0598060:CDS
TCTATGGAACAATTGAGGCA+TGG 0.634765 4_2:+32027439 Msa0598060:CDS
TGAAGAAGATAGGCAACACT+GGG 0.645980 4_2:+32027479 Msa0598060:CDS
TTCCAATGTAGTAGTGAACA+GGG 0.647492 4_2:+32028963 Msa0598060:CDS
CAACACCGATGGAATTCATG+TGG 0.652375 4_2:+32028082 Msa0598060:CDS
CGAGCTATAGATATCACTTG+CGG 0.678372 4_2:+32028313 Msa0598060:CDS
GTGATTCTAGAACCAGAAGG+TGG 0.703740 4_2:+32029769 Msa0598060:CDS
GATGTGATTCTAGAACCAGA+AGG 0.739033 4_2:+32029766 Msa0598060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAACAATAACATATA+TGG + chr4_2:32026861-32026880 Msa0598060:intron 10.0%
!! AAAATATAACAAATGAAAAT+TGG - chr4_2:32027643-32027662 None:intergenic 10.0%
!! ACATTCAAAAAATAAATATT+TGG + chr4_2:32028187-32028206 Msa0598060:intron 10.0%
!!! ATATATTTGAAGTATTTTTT+TGG + chr4_2:32028624-32028643 Msa0598060:intron 10.0%
!! ATTGGAAAGAAAAAAAAAAA+AGG - chr4_2:32027625-32027644 None:intergenic 15.0%
!!! AAAAGAATGATTTCAAATTT+TGG + chr4_2:32027699-32027718 Msa0598060:intron 15.0%
!!! ATACTTGTTGTAAAAATTTA+TGG - chr4_2:32027875-32027894 None:intergenic 15.0%
!! AATGGAAAATAAATTTATGT+AGG + chr4_2:32027920-32027939 Msa0598060:intron 15.0%
!! CTAAATAAAAAACATTCAAT+TGG + chr4_2:32028213-32028232 Msa0598060:intron 15.0%
!!! ATTGATTAAAACTTTTTCTT+TGG + chr4_2:32028426-32028445 Msa0598060:intron 15.0%
!! AATTTCAACATATATGAAAA+AGG + chr4_2:32028533-32028552 Msa0598060:intron 15.0%
!!! GAGGAAATTTAAAATAAATA+AGG + chr4_2:32028601-32028620 Msa0598060:intron 15.0%
!!! TTGAATAACAATTTTTCATA+GGG - chr4_2:32028818-32028837 None:intergenic 15.0%
!!! TTTGAATAACAATTTTTCAT+AGG - chr4_2:32028819-32028838 None:intergenic 15.0%
!!! TTTTTTTATATTTCCTTTTC+TGG + chr4_2:32028861-32028880 Msa0598060:intron 15.0%
!! ATTTAGAAGAAATTAATAAG+TGG - chr4_2:32029363-32029382 None:intergenic 15.0%
!! TTTCTATGGTTATTAATTAA+AGG - chr4_2:32029399-32029418 None:intergenic 15.0%
!!! AAAACAAAACATCATCATTT+TGG + chr4_2:32026729-32026748 Msa0598060:CDS 20.0%
!!! TCAGTAATGCAATTTTAAAT+TGG - chr4_2:32027140-32027159 None:intergenic 20.0%
!!! TAATGAATTGATTTTGAACA+TGG - chr4_2:32027385-32027404 None:intergenic 20.0%
!!! CTAATTGATTCATACTTTAA+TGG + chr4_2:32027902-32027921 Msa0598060:intron 20.0%
!! ACATAATAAACTGTGATATT+GGG + chr4_2:32028123-32028142 Msa0598060:CDS 20.0%
!!! TTTACTTTCTTTTTTGTTGA+AGG + chr4_2:32028250-32028269 Msa0598060:intron 20.0%
!!! ATTAAAACTTTTTCTTTGGA+GGG + chr4_2:32028430-32028449 Msa0598060:intron 20.0%
!!! TAAAACTTTAAAGTTGAGAT+TGG + chr4_2:32028462-32028481 Msa0598060:intron 20.0%
!!! AAAACTTTAAAGTTGAGATT+GGG + chr4_2:32028463-32028482 Msa0598060:intron 20.0%
!! ATAGATCTCAAAAATTAAGA+TGG + chr4_2:32028503-32028522 Msa0598060:intron 20.0%
!!! TATTTGAAGTATTTTTTTGG+TGG + chr4_2:32028627-32028646 Msa0598060:intron 20.0%
!! TAATATATATTCTGCAGTAT+TGG + chr4_2:32028907-32028926 Msa0598060:intron 20.0%
!!! ATTTTGAAAATTAGTCTGTT+AGG + chr4_2:32029051-32029070 Msa0598060:intron 20.0%
!!! TAGTATTGATCTATGATTAT+GGG - chr4_2:32029595-32029614 None:intergenic 20.0%
!! AATGTTAGATATGTAAATAG+AGG + chr4_2:32029817-32029836 Msa0598060:CDS 20.0%
! TTCATTTAATGTCTCCTTTA+AGG + chr4_2:32026955-32026974 Msa0598060:intron 25.0%
! CGTAATTAACTGAATTATTG+AGG - chr4_2:32027249-32027268 None:intergenic 25.0%
! TTCAGTTAATTACGTTACTA+TGG + chr4_2:32027255-32027274 Msa0598060:intron 25.0%
! TTACGTTACTATGGATAAAT+CGG + chr4_2:32027264-32027283 Msa0598060:intron 25.0%
! TTACTTTGTGAACTAATCTA+TGG - chr4_2:32027313-32027332 None:intergenic 25.0%
!! TTGATTTTGAACATGGTATA+AGG - chr4_2:32027378-32027397 None:intergenic 25.0%
! AAAACTTACAAGGTTATTGT+TGG - chr4_2:32027598-32027617 None:intergenic 25.0%
!! AAAAGGTACAAAAACTTACA+AGG - chr4_2:32027608-32027627 None:intergenic 25.0%
! TTATGAAAAATACTTGAGAG+AGG + chr4_2:32027676-32027695 Msa0598060:intron 25.0%
! GGAAAATAAATTTATGTAGG+CGG + chr4_2:32027923-32027942 Msa0598060:intron 25.0%
! ATTTGAGAGTAAAGAATCTA+AGG + chr4_2:32027967-32027986 Msa0598060:CDS 25.0%
! GACATAATAAACTGTGATAT+TGG + chr4_2:32028122-32028141 Msa0598060:CDS 25.0%
! CTCAATCTATGAATTAAATG+AGG - chr4_2:32028381-32028400 None:intergenic 25.0%
!!! GATTAAAACTTTTTCTTTGG+AGG + chr4_2:32028429-32028448 Msa0598060:intron 25.0%
!! TAAGTTATTTGCCAACTTTT+AGG + chr4_2:32029196-32029215 Msa0598060:intron 25.0%
! ATAACCGATAAAACAAACAT+TGG + chr4_2:32029222-32029241 Msa0598060:intron 25.0%
!!! CTTTCCAATGTTTGTTTTAT+CGG - chr4_2:32029229-32029248 None:intergenic 25.0%
!!! AGTATTTTGGTCAATTTGTA+TGG - chr4_2:32029454-32029473 None:intergenic 25.0%
! ATATTGTAATAAAACTCACG+TGG - chr4_2:32029477-32029496 None:intergenic 25.0%
!!! GTAGTATTGATCTATGATTA+TGG - chr4_2:32029596-32029615 None:intergenic 25.0%
!!! TGATGTTTTTGTACAAAACT+TGG - chr4_2:32029669-32029688 None:intergenic 25.0%
! ATCATGAGAAGTGTGATAAT+TGG - chr4_2:32026606-32026625 None:intergenic 30.0%
!! ATCATTTTGGTCACATAACT+AGG + chr4_2:32026742-32026761 Msa0598060:CDS 30.0%
ACATATATGGCAACAAAAAC+AGG + chr4_2:32026874-32026893 Msa0598060:intron 30.0%
AGATGGTATATCCTATTCTT+GGG - chr4_2:32027034-32027053 None:intergenic 30.0%
!! AAAAAGTTATTGGCTTGAGA+TGG - chr4_2:32027051-32027070 None:intergenic 30.0%
TTCACAAAGTAAAGCTTAGT+GGG + chr4_2:32027321-32027340 Msa0598060:intron 30.0%
! ATTTTGAACATGGTATAAGG+AGG - chr4_2:32027375-32027394 None:intergenic 30.0%
!! AATTCATCATGTTAGCTCTA+TGG + chr4_2:32027423-32027442 Msa0598060:intron 30.0%
GTCTCAAATTTCAAAGTTGA+TGG + chr4_2:32027516-32027535 Msa0598060:CDS 30.0%
!! TCAAATTTCAAAGTTGATGG+TGG + chr4_2:32027519-32027538 Msa0598060:CDS 30.0%
GTTATTGTTGGAATATTTGC+AGG - chr4_2:32027586-32027605 None:intergenic 30.0%
GAAGAATATGATCTTACAGT+TGG - chr4_2:32027849-32027868 None:intergenic 30.0%
GATTGTATTTCGATAGTTAG+TGG + chr4_2:32028277-32028296 Msa0598060:CDS 30.0%
! ATCTATAGCTCGAACATTTT+TGG - chr4_2:32028305-32028324 None:intergenic 30.0%
TTAGTTAAGGAAAGCAAAAG+AGG + chr4_2:32028582-32028601 Msa0598060:intron 30.0%
!!! TTGAAGTATTTTTTTGGTGG+TGG + chr4_2:32028630-32028649 Msa0598060:intron 30.0%
ACAATGCAGAAAAATATGCA+AGG - chr4_2:32028675-32028694 None:intergenic 30.0%
ACATTAACAGGAACAACTAA+TGG + chr4_2:32028988-32029007 Msa0598060:CDS 30.0%
ATGGAGTGAGAATAAAAACT+TGG + chr4_2:32029007-32029026 Msa0598060:CDS 30.0%
GAACATAGAAATGCAAAATG+TGG + chr4_2:32029564-32029583 Msa0598060:CDS 30.0%
! GTTTTGTACAAAAACATCAG+AGG + chr4_2:32029670-32029689 Msa0598060:CDS 30.0%
TGCATATGAGTAATGAAGCT+AGG - chr4_2:32026632-32026651 None:intergenic 35.0%
GCTTCATTACTCATATGCAA+TGG + chr4_2:32026633-32026652 Msa0598060:CDS 35.0%
ATGGTAGTAACGTTCAACAT+TGG - chr4_2:32026688-32026707 None:intergenic 35.0%
TCAATCTACCATCATGTACA+TGG - chr4_2:32026707-32026726 None:intergenic 35.0%
! CTTTTCTCTTCAAAGTCTCA+AGG + chr4_2:32026780-32026799 Msa0598060:CDS 35.0%
!! ATGGTTAGTGTTGATGACTT+TGG + chr4_2:32026804-32026823 Msa0598060:CDS 35.0%
!! CTCCTTTAAGGCATTTAAGA+AGG + chr4_2:32026967-32026986 Msa0598060:intron 35.0%
TGCCTTCTTAAATGCCTTAA+AGG - chr4_2:32026972-32026991 None:intergenic 35.0%
! TTAAGGCATTTAAGAAGGCA+TGG + chr4_2:32026972-32026991 Msa0598060:intron 35.0%
TGGAATGAAGCATGTTCTAA+AGG + chr4_2:32026992-32027011 Msa0598060:CDS 35.0%
! GGAATGAAGCATGTTCTAAA+GGG + chr4_2:32026993-32027012 Msa0598060:CDS 35.0%
GAGATGGTATATCCTATTCT+TGG - chr4_2:32027035-32027054 None:intergenic 35.0%
!! CTCAAGCCAATAACTTTTTC+AGG + chr4_2:32027052-32027071 Msa0598060:CDS 35.0%
CATGGACCTGAAAAAGTTAT+TGG - chr4_2:32027061-32027080 None:intergenic 35.0%
TCAACCAAATACTGCCTTTA+TGG + chr4_2:32027081-32027100 Msa0598060:CDS 35.0%
TAGTGTAAGACTCACCATAA+AGG - chr4_2:32027098-32027117 None:intergenic 35.0%
! GGTTTTTGTTGCATTACTGA+AGG - chr4_2:32027199-32027218 None:intergenic 35.0%
ACCCTCAATAACATTACTGA+CGG + chr4_2:32027216-32027235 Msa0598060:intron 35.0%
TCCGTCAGTAATGTTATTGA+GGG - chr4_2:32027220-32027239 None:intergenic 35.0%
TTCCGTCAGTAATGTTATTG+AGG - chr4_2:32027221-32027240 None:intergenic 35.0%
CAGATTTGTCAATTGAGTAC+TGG - chr4_2:32027290-32027309 None:intergenic 35.0%
GTTCACAAAGTAAAGCTTAG+TGG + chr4_2:32027320-32027339 Msa0598060:intron 35.0%
!! TTAGCTCTATGGAACAATTG+AGG + chr4_2:32027434-32027453 Msa0598060:intron 35.0%
TGTCAGCATATGAAGAAGAT+AGG + chr4_2:32027469-32027488 Msa0598060:CDS 35.0%
!! AATTTCAAAGTTGATGGTGG+TGG + chr4_2:32027522-32027541 Msa0598060:CDS 35.0%
TCAATGGCAATGGGAAAAAA+TGG + chr4_2:32027550-32027569 Msa0598060:CDS 35.0%
! TATGATCTTACAGTTGGTCT+TGG - chr4_2:32027843-32027862 None:intergenic 35.0%
! AATGCTACATGCATTTGTTG+TGG - chr4_2:32028002-32028021 None:intergenic 35.0%
ATAAACTGTGATATTGGGAC+AGG + chr4_2:32028128-32028147 Msa0598060:CDS 35.0%
! TTTTCATAGGGTCATCTAAC+CGG - chr4_2:32028806-32028825 None:intergenic 35.0%
CTCTATAAGTAGTCCAGAAA+AGG - chr4_2:32028877-32028896 None:intergenic 35.0%
! TTCTGCAGTATTGGAAGTTT+AGG + chr4_2:32028916-32028935 Msa0598060:intron 35.0%
TTTCCAATGTAGTAGTGAAC+AGG + chr4_2:32028962-32028981 Msa0598060:CDS 35.0%
TTCCAATGTAGTAGTGAACA+GGG + chr4_2:32028963-32028982 Msa0598060:CDS 35.0%
AGTGAGAATAAAAACTTGGC+AGG + chr4_2:32029011-32029030 Msa0598060:CDS 35.0%
AAAACTTGGCAGGTAAAAGT+TGG + chr4_2:32029021-32029040 Msa0598060:intron 35.0%
TTGACAAATCTCCACTTTGT+TGG + chr4_2:32029273-32029292 Msa0598060:intron 35.0%
TGAAAAAAGGACCAACAAAG+TGG - chr4_2:32029287-32029306 None:intergenic 35.0%
!! ACGGTTTGGTCAATGAAAAA+AGG - chr4_2:32029300-32029319 None:intergenic 35.0%
GTTAGGTAGTTTGAACTAGA+CGG - chr4_2:32029319-32029338 None:intergenic 35.0%
AAGTGGATAAAACTTGTCAC+AGG - chr4_2:32029346-32029365 None:intergenic 35.0%
! AGGAGAAGAGCAATTTTCTA+TGG - chr4_2:32029413-32029432 None:intergenic 35.0%
!! AAAACTCACGTGGAGTATTT+TGG - chr4_2:32029467-32029486 None:intergenic 35.0%
TTATGATTCCATATGATCGC+AGG + chr4_2:32029507-32029526 Msa0598060:intron 35.0%
TATGATTCCATATGATCGCA+GGG + chr4_2:32029508-32029527 Msa0598060:intron 35.0%
CAAAACTTGGCTTAATTGCA+CGG - chr4_2:32029656-32029675 None:intergenic 35.0%
TGCAAGTATATTTCTCTGCA+AGG - chr4_2:32029745-32029764 None:intergenic 35.0%
TTGCAGAGAAATATACTTGC+AGG + chr4_2:32029744-32029763 Msa0598060:CDS 35.0%
ATGCAATGGCTCTTACATAG+TGG + chr4_2:32026647-32026666 Msa0598060:CDS 40.0%
!! TCAACATTGGCTTGATGAAG+TGG - chr4_2:32026675-32026694 None:intergenic 40.0%
CGTTACTACCATGTACATGA+TGG + chr4_2:32026696-32026715 Msa0598060:CDS 40.0%
CTCTTCAAAGTCTCAAGGAA+TGG + chr4_2:32026785-32026804 Msa0598060:CDS 40.0%
AGATGGAAGAGATGATAGAG+AGG + chr4_2:32026836-32026855 Msa0598060:CDS 40.0%
! TTCTAAAGGGTCTATCCTTG+TGG + chr4_2:32027006-32027025 Msa0598060:CDS 40.0%
AAGGCAGTATTTGGTTGACA+TGG - chr4_2:32027079-32027098 None:intergenic 40.0%
CTCACCATAAAGGCAGTATT+TGG - chr4_2:32027088-32027107 None:intergenic 40.0%
AGACCCTCTGTAATTACTGA+CGG + chr4_2:32027167-32027186 Msa0598060:intron 40.0%
AGACCGTCAGTAATTACAGA+GGG - chr4_2:32027173-32027192 None:intergenic 40.0%
TAGACCGTCAGTAATTACAG+AGG - chr4_2:32027174-32027193 None:intergenic 40.0%
TCTATGGAACAATTGAGGCA+TGG + chr4_2:32027439-32027458 Msa0598060:CDS 40.0%
ATGAAGAAGATAGGCAACAC+TGG + chr4_2:32027478-32027497 Msa0598060:CDS 40.0%
TGAAGAAGATAGGCAACACT+GGG + chr4_2:32027479-32027498 Msa0598060:CDS 40.0%
ATGGCAATGGGAAAAAATGG+TGG + chr4_2:32027553-32027572 Msa0598060:CDS 40.0%
CTCCTCTCTAATTTCGTATG+AGG - chr4_2:32027725-32027744 None:intergenic 40.0%
! ACATGAATTCCATCGGTGTT+GGG - chr4_2:32028083-32028102 None:intergenic 40.0%
TTCAGCCACATGAATTCCAT+CGG - chr4_2:32028090-32028109 None:intergenic 40.0%
CGAGCTATAGATATCACTTG+CGG + chr4_2:32028313-32028332 Msa0598060:CDS 40.0%
ATGAAACCTGATTCCATGTC+CGG - chr4_2:32028341-32028360 None:intergenic 40.0%
AGAAAAATATGCAAGGTCCG+GGG - chr4_2:32028668-32028687 None:intergenic 40.0%
CAGAAAAATATGCAAGGTCC+GGG - chr4_2:32028669-32028688 None:intergenic 40.0%
GCAGAAAAATATGCAAGGTC+CGG - chr4_2:32028670-32028689 None:intergenic 40.0%
TCGGTTATGCACCTAAAAGT+TGG - chr4_2:32029210-32029229 None:intergenic 40.0%
GTAGTTTGAACTAGACGGTT+TGG - chr4_2:32029314-32029333 None:intergenic 40.0%
AACTTGTCACAGGAGTTGTT+AGG - chr4_2:32029336-32029355 None:intergenic 40.0%
!!! GGAAGTGAGCGTTTTTTGTT+AGG - chr4_2:32029433-32029452 None:intergenic 40.0%
!! GATGTGATTCTAGAACCAGA+AGG + chr4_2:32029766-32029785 Msa0598060:CDS 40.0%
GTGGCACTTTCAATGGCAAT+GGG + chr4_2:32027541-32027560 Msa0598060:CDS 45.0%
GGCCTCATACGAAATTAGAG+AGG + chr4_2:32027720-32027739 Msa0598060:intron 45.0%
GGCTGTGTTCAACAAGAAGA+GGG - chr4_2:32027807-32027826 None:intergenic 45.0%
TGGCTGTGTTCAACAAGAAG+AGG - chr4_2:32027808-32027827 None:intergenic 45.0%
GGTCTTGGTTCATCATTGCA+TGG - chr4_2:32027828-32027847 None:intergenic 45.0%
CACATGAATTCCATCGGTGT+TGG - chr4_2:32028084-32028103 None:intergenic 45.0%
CAACACCGATGGAATTCATG+TGG + chr4_2:32028082-32028101 Msa0598060:CDS 45.0%
ATAGATATCACTTGCGGACC+CGG + chr4_2:32028319-32028338 Msa0598060:CDS 45.0%
TGAAACCTGATTCCATGTCC+GGG - chr4_2:32028340-32028359 None:intergenic 45.0%
GCTTTCCTTAACTAATGCCC+CGG - chr4_2:32028577-32028596 None:intergenic 45.0%
! TGAGCTTAGCTCAGTTGGTA+TGG - chr4_2:32028698-32028717 None:intergenic 45.0%
AGCCCTGTTCACTACTACAT+TGG - chr4_2:32028968-32028987 None:intergenic 45.0%
GTGAACAGGGCTACATTAAC+AGG + chr4_2:32028976-32028995 Msa0598060:CDS 45.0%
TTTCTCTGCAAGGAACGCTT+CGG - chr4_2:32029735-32029754 None:intergenic 45.0%
!! GTGATTCTAGAACCAGAAGG+TGG + chr4_2:32029769-32029788 Msa0598060:CDS 45.0%
!! ATTCTAGAACCAGAAGGTGG+TGG + chr4_2:32029772-32029791 Msa0598060:CDS 45.0%
!! GACTTTGGAGCAAGAGCAGA+TGG + chr4_2:32026819-32026838 Msa0598060:CDS 50.0%
TCCTTGTGGTGCCCAAGAAT+AGG + chr4_2:32027020-32027039 Msa0598060:CDS 50.0%
TCCTATTCTTGGGCACCACA+AGG - chr4_2:32027024-32027043 None:intergenic 50.0%
!! GATGGTGGTGGCACTTTCAA+TGG + chr4_2:32027534-32027553 Msa0598060:CDS 50.0%
GGTGGCACTTTCAATGGCAA+TGG + chr4_2:32027540-32027559 Msa0598060:CDS 50.0%
!! TTTGATTGTGAGAGCGCCAG+AGG + chr4_2:32028052-32028071 Msa0598060:CDS 50.0%
GTAGTGCTAACCGATGCTTC+CGG + chr4_2:32028555-32028574 Msa0598060:intron 50.0%
TAGTGCTAACCGATGCTTCC+GGG + chr4_2:32028556-32028575 Msa0598060:intron 50.0%
! AACTAATGCCCCGGAAGCAT+CGG - chr4_2:32028568-32028587 None:intergenic 50.0%
! TGCTTCCGGGGCATTAGTTA+AGG + chr4_2:32028569-32028588 Msa0598060:intron 50.0%
AAGGTCCGGGGTTCAAATCT+CGG - chr4_2:32028656-32028675 None:intergenic 50.0%
CATCGTGAGCTTAGCTCAGT+TGG - chr4_2:32028703-32028722 None:intergenic 50.0%
TAACCAGTGTCTCGATGCAC+CGG + chr4_2:32028784-32028803 Msa0598060:intron 50.0%
GATTCCATATGATCGCAGGG+TGG + chr4_2:32029511-32029530 Msa0598060:intron 50.0%
AGAACCACCCTGCGATCATA+TGG - chr4_2:32029518-32029537 None:intergenic 50.0%
ATATGATCGCAGGGTGGTTC+TGG + chr4_2:32029517-32029536 Msa0598060:intron 50.0%
AAACAGAGCCATGCCAAGAG+CGG + chr4_2:32029623-32029642 Msa0598060:CDS 50.0%
!! TCGGTGTTGGGACTATCCTC+TGG - chr4_2:32028071-32028090 None:intergenic 55.0%
GAGGATAGTCCCAACACCGA+TGG + chr4_2:32028071-32028090 Msa0598060:CDS 55.0%
AGTGCTAACCGATGCTTCCG+GGG + chr4_2:32028557-32028576 Msa0598060:intron 55.0%
TAACCGGTGCATCGAGACAC+TGG - chr4_2:32028790-32028809 None:intergenic 55.0%
! GCTCTTGGCATGGCTCTGTT+TGG - chr4_2:32029624-32029643 None:intergenic 55.0%
! GCTTTGTTCCGCTCTTGGCA+TGG - chr4_2:32029634-32029653 None:intergenic 55.0%
! CTAGAACCAGAAGGTGGTGG+AGG + chr4_2:32029775-32029794 Msa0598060:CDS 55.0%
GTAGTTCCTCCACCACCTTC+TGG - chr4_2:32029784-32029803 None:intergenic 55.0%
ATCACTTGCGGACCCGGACA+TGG + chr4_2:32028325-32028344 Msa0598060:CDS 60.0%
GGTGGCCGAGATTTGAACCC+CGG + chr4_2:32028648-32028667 Msa0598060:intron 60.0%
!! GCACGGCTTTGTTCCGCTCT+TGG - chr4_2:32029639-32029658 None:intergenic 60.0%
GCGGACCCGGACATGGAATC+AGG + chr4_2:32028332-32028351 Msa0598060:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 32026588 32029870 32026588 ID=Msa0598060;Name=Msa0598060
chr4_2 mRNA 32026588 32029870 32026588 ID=Msa0598060-mRNA-1;Parent=Msa0598060;Name=Msa0598060-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.71|0.5|1|1|1|8|0|444
chr4_2 exon 32026588 32026857 32026588 ID=Msa0598060-mRNA-1:exon:7824;Parent=Msa0598060-mRNA-1
chr4_2 exon 32026977 32027102 32026977 ID=Msa0598060-mRNA-1:exon:7825;Parent=Msa0598060-mRNA-1
chr4_2 exon 32027438 32027608 32027438 ID=Msa0598060-mRNA-1:exon:7826;Parent=Msa0598060-mRNA-1
chr4_2 exon 32027825 32027851 32027825 ID=Msa0598060-mRNA-1:exon:7827;Parent=Msa0598060-mRNA-1
chr4_2 exon 32027942 32028149 32027942 ID=Msa0598060-mRNA-1:exon:7828;Parent=Msa0598060-mRNA-1
chr4_2 exon 32028272 32028353 32028272 ID=Msa0598060-mRNA-1:exon:7829;Parent=Msa0598060-mRNA-1
chr4_2 exon 32028924 32029032 32028924 ID=Msa0598060-mRNA-1:exon:7830;Parent=Msa0598060-mRNA-1
chr4_2 exon 32029529 32029870 32029529 ID=Msa0598060-mRNA-1:exon:7831;Parent=Msa0598060-mRNA-1
chr4_2 CDS 32026588 32026857 32026588 ID=Msa0598060-mRNA-1:cds;Parent=Msa0598060-mRNA-1
chr4_2 CDS 32026977 32027102 32026977 ID=Msa0598060-mRNA-1:cds;Parent=Msa0598060-mRNA-1
chr4_2 CDS 32027438 32027608 32027438 ID=Msa0598060-mRNA-1:cds;Parent=Msa0598060-mRNA-1
chr4_2 CDS 32027825 32027851 32027825 ID=Msa0598060-mRNA-1:cds;Parent=Msa0598060-mRNA-1
chr4_2 CDS 32027942 32028149 32027942 ID=Msa0598060-mRNA-1:cds;Parent=Msa0598060-mRNA-1
chr4_2 CDS 32028272 32028353 32028272 ID=Msa0598060-mRNA-1:cds;Parent=Msa0598060-mRNA-1
chr4_2 CDS 32028924 32029032 32028924 ID=Msa0598060-mRNA-1:cds;Parent=Msa0598060-mRNA-1
chr4_2 CDS 32029529 32029870 32029529 ID=Msa0598060-mRNA-1:cds;Parent=Msa0598060-mRNA-1
Gene Sequence

>Msa0598060

ATGTATCAACAAAAGCCAATTATCACACTTCTCATGATATTCCTAGCTTCATTACTCATATGCAATGGCTCTTACATAGTGGAACCACTTCATCAAGCCAATGTTGAACGTTACTACCATGTACATGATGGTAGATTGATCAAAACAAAACATCATCATTTTGGTCACATAACTAGGACAAATAGAGATACACTTTTCTCTTCAAAGTCTCAAGGAATGGTTAGTGTTGATGACTTTGGAGCAAGAGCAGATGGAAGAGATGATAGAGAGGCATTTAAGAAGGCATGGAATGAAGCATGTTCTAAAGGGTCTATCCTTGTGGTGCCCAAGAATAGGATATACCATCTCAAGCCAATAACTTTTTCAGGTCCATGTCAACCAAATACTGCCTTTATGCTCTATGGAACAATTGAGGCATGGTCTCAAATGTCAGCATATGAAGAAGATAGGCAACACTGGGTTGTGTTTGATAGTGTCTCAAATTTCAAAGTTGATGGTGGTGGCACTTTCAATGGCAATGGGAAAAAATGGTGGCAAAACTCCTGCAAATATTCCAACAATAACCTTCCATGCAATGATGAACCAAGACCAACTGCGGTGACATTCTATGATTGCAAAAATTTGAGAGTAAAGAATCTAAGGTTCAAAGATCCACAACAAATGCATGTAGCATTTGAGAGATGCTTCAATGTGTTTGTTTCGAATTTGATTGTGAGAGCGCCAGAGGATAGTCCCAACACCGATGGAATTCATGTGGCTGAAACACAAAATATTGACATAATAAACTGTGATATTGGGACAGGTGATGATTGTATTTCGATAGTTAGTGGATCCAAAAATGTTCGAGCTATAGATATCACTTGCGGACCCGGACATGGAATCAGTATTGGAAGTTTAGGAGCTGATAACTCAGAAGCTGAAGTTTCCAATGTAGTAGTGAACAGGGCTACATTAACAGGAACAACTAATGGAGTGAGAATAAAAACTTGGCAGGGTGGTTCTGGATATGCAAGAAACATCAAATTTATGAACATAGAAATGCAAAATGTGGCAAATCCCATAATCATAGATCAATACTACTGCGACCAAACAGAGCCATGCCAAGAGCGGAACAAAGCCGTGCAATTAAGCCAAGTTTTGTACAAAAACATCAGAGGAACGAGTGCTTCAGAAGTAGCGATCAAATTTAATTGCAGCCGAAGCGTTCCTTGCAGAGAAATATACTTGCAGGATGTGATTCTAGAACCAGAAGGTGGTGGAGGAACTACTGCAACATGTGAGAATGTTAGATATGTAAATAGAGGAAAGTTTTATCCTCAATGCTCTCCACAATAG
Protein sequence

>Msa0598060

MYQQKPIITLLMIFLASLLICNGSYIVEPLHQANVERYYHVHDGRLIKTKHHHFGHITRTNRDTLFSSKSQGMVSVDDFGARADGRDDREAFKKAWNEACSKGSILVVPKNRIYHLKPITFSGPCQPNTAFMLYGTIEAWSQMSAYEEDRQHWVVFDSVSNFKVDGGGTFNGNGKKWWQNSCKYSNNNLPCNDEPRPTAVTFYDCKNLRVKNLRFKDPQQMHVAFERCFNVFVSNLIVRAPEDSPNTDGIHVAETQNIDIINCDIGTGDDCISIVSGSKNVRAIDITCGPGHGISIGSLGADNSEAEVSNVVVNRATLTGTTNGVRIKTWQGGSGYARNIKFMNIEMQNVANPIIIDQYYCDQTEPCQERNKAVQLSQVLYKNIRGTSASEVAIKFNCSRSVPCREIYLQDVILEPEGGGGTTATCENVRYVNRGKFYPQCSPQ*