AlfalfaGEDB | Msa0683300

M. sativa cultivar zhongmu-4 / Msa0683300

KEGG: K09284
GO: -
PFAM: PF00847.23

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0683300	XP_013456044.1	96.429	308	10	1	3	310	69	375	0.0	620

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0683300	sp\|Q84TB5\|AP22_ORYSJ	75.610	205	49	1	42	245	94	298	2.97e-99	302

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0683300	A0A072UW41	96.429	308	10	1	3	310	69	375	0.0	620

TFs/TRs:

Gene ID	Type	Classification
Msa0683300	TF	AP2/ERF-AP2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0683300	Msa0784860	0.808078	3.695135e-50	-8.615850e-47
Msa0683300	Msa0870150	0.803392	3.564142e-49	-8.615850e-47
Msa0683300	Msa1260130	0.824732	6.881609e-54	-8.615850e-47
Msa0683300	Msa1465960	0.822393	2.426517e-53	-8.615850e-47
Msa0429290	Msa0683300	0.807594	4.682694e-50	-8.615850e-47
Msa0475580	Msa0683300	0.803250	3.812140e-49	-8.615850e-47
Msa0519220	Msa0683300	0.800476	1.415855e-48	-8.615850e-47
Msa0545080	Msa0683300	0.856315	3.496162e-62	-8.615850e-47
Msa0557480	Msa0683300	0.815166	1.062666e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0683300	MtrunA17_Chr4g0030191	96.429	308	10	1	3	310	69	375	0.0	620
Msa0683300	MtrunA17_Chr2g0325911	64.762	315	88	5	2	310	67	364	6.60e-119	350
Msa0683300	MtrunA17_Chr7g0263581	74.583	240	58	2	9	245	79	318	6.23e-105	315
Msa0683300	MtrunA17_Chr5g0401891	66.797	256	70	2	1	241	72	327	3.53e-98	299
Msa0683300	MtrunA17_Chr4g0053181	78.788	198	41	1	51	247	161	358	1.71e-93	287
Msa0683300	MtrunA17_Chr3g0126151	93.617	94	6	0	104	197	47	140	2.94e-59	187
Msa0683300	MtrunA17_Chr1g0170761	45.833	288	123	9	8	287	80	342	1.91e-56	187
Msa0683300	MtrunA17_Chr7g0216601	47.959	196	91	2	63	247	65	260	1.88e-53	180
Msa0683300	MtrunA17_Chr4g0054351	51.685	178	75	3	63	229	310	487	1.62e-52	183
Msa0683300	MtrunA17_Chr5g0400871	52.047	171	71	3	63	222	249	419	2.14e-50	175
Msa0683300	MtrunA17_Chr4g0072291	51.479	169	71	3	63	220	159	327	3.71e-50	174
Msa0683300	MtrunA17_Chr7g0249621	48.370	184	82	4	63	233	259	442	3.19e-49	174
Msa0683300	MtrunA17_Chr7g0249621	43.956	91	46	2	42	127	337	427	2.46e-12	67.8
Msa0683300	MtrunA17_Chr3g0135161	50.838	179	78	4	63	231	310	488	3.64e-49	174
Msa0683300	MtrunA17_Chr4g0032631	42.731	227	114	5	19	232	122	345	7.69e-49	171
Msa0683300	MtrunA17_Chr8g0355191	45.116	215	104	3	48	251	40	251	5.77e-48	166
Msa0683300	MtrunA17_Chr8g0343851	51.807	166	72	3	63	220	248	413	9.78e-48	169
Msa0683300	MtrunA17_Chr8g0343851	43.956	91	46	2	42	127	323	413	9.14e-12	65.9
Msa0683300	MtrunA17_Chr4g0073521	50.292	171	77	3	57	219	41	211	5.67e-47	162
Msa0683300	MtrunA17_Chr6g0452911	48.066	181	76	4	67	229	65	245	6.64e-47	162
Msa0683300	MtrunA17_Chr6g0452911	40.000	110	58	4	64	165	171	280	3.28e-11	63.9
Msa0683300	MtrunA17_Chr1g0152161	49.162	179	81	4	63	231	312	490	9.29e-47	167
Msa0683300	MtrunA17_Chr1g0152161	39.216	102	57	2	31	127	376	477	1.63e-12	68.2
Msa0683300	MtrunA17_Chr8g0355181	37.367	281	152	5	40	302	23	297	5.20e-46	160
Msa0683300	MtrunA17_Chr4g0002011	52.874	174	71	3	57	219	30	203	8.85e-46	158
Msa0683300	MtrunA17_Chr2g0328971	42.793	222	108	6	24	232	131	346	3.14e-45	161
Msa0683300	MtrunA17_Chr5g0412151	45.815	227	106	6	31	240	188	414	1.87e-43	156
Msa0683300	MtrunA17_Chr8g0367311	55.970	134	58	1	90	222	19	152	3.65e-43	149
Msa0683300	MtrunA17_Chr8g0362541	46.369	179	74	3	63	219	69	247	1.83e-37	138
Msa0683300	MtrunA17_Chr7g0257361	49.286	140	54	3	63	189	57	192	2.98e-27	110
Msa0683300	MtrunA17_Chr2g0305821	46.094	128	57	3	47	162	26	153	6.21e-20	85.1
Msa0683300	MtrunA17_Chr2g0305831	56.364	55	24	0	165	219	5	59	1.92e-13	68.6
Msa0683300	MtrunA17_Chr4g0063751	48.684	76	38	1	156	230	4	79	1.38e-12	66.2
Msa0683300	MtrunA17_Chr8g0375031	50.000	74	30	3	66	133	124	196	3.17e-11	62.4
Msa0683300	MtrunA17_Chr5g0422501	40.404	99	51	4	55	149	98	192	6.70e-11	62.8
Msa0683300	MtrunA17_Chr4g0044881	48.000	75	34	3	61	133	108	179	9.80e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0683300	AT2G28550.3	63.259	313	90	9	10	306	85	388	5.01e-104	313
Msa0683300	AT4G36920.1	66.403	253	68	3	6	241	57	309	9.48e-95	288
Msa0683300	AT4G36920.2	66.403	253	68	3	6	241	57	309	9.48e-95	288
Msa0683300	AT2G28550.1	58.786	313	89	10	10	306	85	373	1.17e-88	273
Msa0683300	AT5G67180.2	66.524	233	75	2	10	241	41	271	4.52e-88	266
Msa0683300	AT5G67180.1	66.524	233	75	2	10	241	41	271	6.95e-88	268
Msa0683300	AT5G67180.3	70.093	214	61	2	10	222	41	252	4.98e-86	260
Msa0683300	AT2G28550.4	69.820	222	50	6	10	216	85	304	3.91e-81	250
Msa0683300	AT5G67180.4	70.526	190	53	2	10	198	41	228	2.77e-73	227
Msa0683300	AT5G60120.1	48.742	318	123	9	9	308	87	382	9.19e-73	233
Msa0683300	AT5G60120.4	51.056	284	100	7	9	275	87	348	3.72e-71	226
Msa0683300	AT5G60120.3	51.056	284	100	7	9	275	87	348	3.72e-71	226
Msa0683300	AT5G60120.5	51.056	284	100	7	9	275	87	348	3.72e-71	226
Msa0683300	AT5G60120.6	54.231	260	81	7	9	251	87	325	3.49e-70	226
Msa0683300	AT5G60120.2	51.408	284	99	8	9	275	87	348	6.20e-70	226
Msa0683300	AT2G28550.2	74.194	186	32	5	10	181	85	268	2.03e-69	218
Msa0683300	AT2G28550.6	74.194	186	32	5	10	181	85	268	2.03e-69	218
Msa0683300	AT2G28550.5	56.637	226	74	6	83	306	4	207	1.57e-63	203
Msa0683300	AT5G57390.2	44.796	221	102	5	40	249	59	270	1.19e-52	179
Msa0683300	AT1G72570.1	48.485	198	82	5	41	220	193	388	3.48e-52	177
Msa0683300	AT5G57390.1	44.796	221	102	5	40	249	177	388	7.16e-52	180
Msa0683300	AT1G51190.1	43.210	243	112	6	3	232	140	369	2.37e-51	179
Msa0683300	AT3G20840.1	49.180	183	80	4	63	232	178	360	1.60e-50	176
Msa0683300	AT3G20840.1	45.055	91	45	2	42	127	256	346	1.39e-12	68.9
Msa0683300	AT4G37750.1	48.901	182	80	3	63	231	280	461	4.62e-49	172
Msa0683300	AT5G17430.2	47.090	189	89	3	42	219	124	312	5.91e-48	169
Msa0683300	AT5G17430.1	47.090	189	89	3	42	219	186	374	1.08e-47	169
Msa0683300	AT2G39250.2	67.164	134	41	1	31	164	76	206	1.92e-47	160
Msa0683300	AT2G39250.1	49.767	215	75	5	31	244	76	258	2.42e-47	162
Msa0683300	AT3G54990.1	52.655	226	78	5	11	235	72	269	4.81e-46	159
Msa0683300	AT3G54990.2	63.190	163	53	2	11	172	72	228	4.96e-46	157
Msa0683300	AT5G10510.7	50.515	194	83	4	63	243	227	420	2.19e-44	159
Msa0683300	AT5G10510.5	50.515	194	83	4	63	243	227	420	2.19e-44	159
Msa0683300	AT5G10510.6	50.515	194	83	4	63	243	265	458	2.88e-44	160
Msa0683300	AT5G10510.4	50.515	194	83	4	63	243	265	458	2.88e-44	160
Msa0683300	AT5G10510.1	50.515	194	83	4	63	243	250	443	4.15e-44	159
Msa0683300	AT3G54320.3	49.718	177	78	2	63	228	62	238	1.44e-43	155
Msa0683300	AT3G54320.1	49.718	177	78	2	63	228	62	238	1.82e-43	155
Msa0683300	AT5G65510.4	52.632	171	70	3	63	222	170	340	1.87e-42	152
Msa0683300	AT5G65510.2	50.273	183	79	4	63	233	228	410	2.59e-42	153
Msa0683300	AT5G65510.1	50.273	183	79	4	63	233	228	410	2.59e-42	153
Msa0683300	AT1G79700.2	53.448	174	70	2	57	219	43	216	3.14e-40	144
Msa0683300	AT5G10510.2	48.969	194	83	5	63	243	227	417	5.07e-40	147
Msa0683300	AT5G65510.3	42.000	200	100	5	3	188	109	306	1.02e-39	142
Msa0683300	AT1G16060.1	52.273	176	73	2	55	219	47	222	2.46e-38	139
Msa0683300	AT5G10510.3	44.393	214	86	5	63	243	265	478	1.61e-37	141
Msa0683300	AT1G79700.1	50.575	174	65	3	57	219	43	206	9.10e-33	124
Msa0683300	AT3G54320.4	50.735	136	66	1	90	224	33	168	6.67e-32	122
Msa0683300	AT3G54320.5	50.735	136	66	1	90	224	33	168	6.67e-32	122
Msa0683300	AT1G79700.3	54.412	136	61	1	85	219	5	140	2.47e-28	110
Msa0683300	AT1G16060.3	54.412	136	61	1	85	219	17	152	4.39e-27	107
Msa0683300	AT2G41710.1	49.286	140	54	3	63	189	67	202	5.48e-27	110
Msa0683300	AT2G41710.4	50.382	131	51	2	63	183	67	193	6.90e-27	110
Msa0683300	AT1G79700.4	54.264	129	58	1	92	219	6	134	5.25e-26	103
Msa0683300	AT2G41710.2	48.529	136	51	3	63	183	67	198	1.13e-24	103
Msa0683300	AT2G41710.3	47.934	121	49	2	73	183	112	228	5.29e-22	96.7
Msa0683300	AT3G54320.2	54.255	94	42	1	136	228	4	97	1.68e-19	87.4
Msa0683300	AT1G16060.2	59.524	84	33	1	137	219	5	88	1.37e-18	83.2
Msa0683300	AT1G16060.4	59.524	84	33	1	137	219	5	88	1.37e-18	83.2
Msa0683300	AT1G16060.6	59.524	84	33	1	137	219	5	88	1.37e-18	83.2
Msa0683300	AT1G16060.5	59.524	84	33	1	137	219	5	88	1.37e-18	83.2
Msa0683300	AT2G47520.1	50.000	74	34	2	50	121	33	105	4.89e-12	63.9
Msa0683300	AT5G25390.2	37.778	90	45	1	156	245	4	82	3.26e-11	62.0
Msa0683300	AT3G25730.1	37.895	95	55	2	55	149	53	143	5.28e-11	63.2
Msa0683300	AT1G68840.2	40.000	95	53	2	55	149	56	146	6.74e-11	63.2
Msa0683300	AT1G68840.1	40.000	95	53	2	55	149	56	146	6.74e-11	63.2

Find 108 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGAAGAGCGTCATTCTTTA+AGG	0.116942	4_4:-45699052	Msa0683300:CDS
TCAAAATCTTGATCTCAATT+TGG	0.185955	4_4:-45699481	Msa0683300:CDS
CAGATGGAAACCAATGTTTC+AGG	0.203372	4_4:-45699110	Msa0683300:CDS
TTGCTTCCCTTCCATTGTTT+TGG	0.205535	4_4:+45699677	None:intergenic
TGAGTTTCGATATTCTAAAA+TGG	0.223508	4_4:-45701103	Msa0683300:exon
TAATGAATTCTATGAGCTTT+TGG	0.241074	4_4:-45698814	Msa0683300:three_prime_UTR
ACAAGAGGGTAACGTGTAAT+TGG	0.305183	4_4:-45698624	Msa0683300:three_prime_UTR
TGCGGGAAACAAGTTTATTT+AGG	0.306046	4_4:-45700657	Msa0683300:intron
ATCGAGCTGCTATTAAGTTT+AGG	0.315665	4_4:-45700416	Msa0683300:CDS
TCGAGCTGCTATTAAGTTTA+GGG	0.321177	4_4:-45700415	Msa0683300:CDS
CAAAATCTTGATCTCAATTT+GGG	0.330247	4_4:-45699480	Msa0683300:CDS
TTTATCGAAGGACTGGTAGA+TGG	0.343892	4_4:-45700870	Msa0683300:CDS
GGCCTGGTCCCAAAGAAAAC+TGG	0.346288	4_4:-45699443	Msa0683300:CDS
CCTGATTCATCCACTGTTTG+CGG	0.351817	4_4:+45701194	None:intergenic
GTAGATGGGAATCGCATATC+TGG	0.376583	4_4:-45700855	Msa0683300:intron
TAATTATTTGTAGGGATTGC+GGG	0.376888	4_4:-45700674	Msa0683300:intron
CTTCCAGCTTGCATAGTGTA+AGG	0.377553	4_4:+45699393	None:intergenic
TCGTGCAGGTATATATATCT+TGG	0.379589	4_4:-45699868	Msa0683300:intron
GACGAAGAATTTATCGAAAG+AGG	0.380753	4_4:-45700255	Msa0683300:CDS
ATGTTTGTTACCCTCATTGA+TGG	0.389849	4_4:+45699606	None:intergenic
TACTTCTACTCAAGAGAAAC+AGG	0.408272	4_4:+45698950	None:intergenic
CATCTCTCCTTCATATGTAC+TGG	0.408785	4_4:+45699639	None:intergenic
CGTGCAGGTATATATATCTT+GGG	0.413611	4_4:-45699867	Msa0683300:intron
GGCATAGCCACGCCAGGGCC+TGG	0.414599	4_4:-45699459	Msa0683300:CDS
CCGCAAACAGTGGATGAATC+AGG	0.419467	4_4:-45701194	Msa0683300:five_prime_UTR
CCTTAAAGAATGACGCTCTT+CGG	0.421745	4_4:+45699052	None:intergenic
ATCACCGATTCCTGAAACAT+TGG	0.426489	4_4:+45699100	None:intergenic
GAGTTTCGATATTCTAAAAT+GGG	0.435101	4_4:-45701102	Msa0683300:exon
GGCGATGCTTTCACTTTCTC+CGG	0.437530	4_4:-45701674	Msa0683300:five_prime_UTR
TTACGATGATGATCTTAAAC+AGG	0.443833	4_4:-45700358	Msa0683300:intron
GCTATTAAGTTTAGGGGAGT+TGG	0.448163	4_4:-45700408	Msa0683300:CDS
GTGAGAGGTGGAGGAAGAAA+CGG	0.453559	4_4:+45701580	None:intergenic
AGATGACGAAATCACCTCAT+TGG	0.464874	4_4:+45699004	None:intergenic
TATCTGTTATTGAGCAATCA+AGG	0.466550	4_4:-45698791	Msa0683300:three_prime_UTR
CTACTCTTCTTCACTTGTTG+TGG	0.469737	4_4:+45700937	None:intergenic
ACTGGAAAAGTGAGAGGTGG+AGG	0.474019	4_4:+45701571	None:intergenic
GACGTAGCTTGCAACTTCAC+AGG	0.475205	4_4:+45701039	None:intergenic
GTTCTGATTCTGAATTGAAT+CGG	0.477951	4_4:+45701247	None:intergenic
TGGCCGTTGGGAAGCTCGAA+TGG	0.481852	4_4:-45700150	Msa0683300:CDS
GAAACCAATGTTTCAGGAAT+CGG	0.487916	4_4:-45699104	Msa0683300:CDS
GTAATTATTTGTAGGGATTG+CGG	0.488911	4_4:-45700675	Msa0683300:intron
GCCTGGTCCCAAAGAAAACT+GGG	0.489302	4_4:-45699442	Msa0683300:CDS
ATCATCATCATCGCCGGCGA+CGG	0.491850	4_4:-45701390	Msa0683300:five_prime_UTR
AATTTGGGCATAGCCACGCC+AGG	0.497057	4_4:-45699465	Msa0683300:CDS
ACTGCACAAATGTGGCCGTT+GGG	0.502208	4_4:-45700162	Msa0683300:CDS
TTTGAGGCCAGTACATATGA+AGG	0.506180	4_4:-45699646	Msa0683300:CDS
CACTGCACAAATGTGGCCGT+TGG	0.520758	4_4:-45700163	Msa0683300:CDS
GCTCGAATGGGACAATTCCT+TGG	0.523960	4_4:-45700137	Msa0683300:CDS
GTCAATCATATTAATGAAGT+CGG	0.528177	4_4:+45698892	None:intergenic
CTCAGAGAACGAGGTTGAGA+GGG	0.529722	4_4:+45701606	None:intergenic
CAAGTGAAGAAGAGTAGAAG+AGG	0.529800	4_4:-45700931	Msa0683300:CDS
TCGGTGATCCATCTTTGAAA+AGG	0.530640	4_4:-45699085	Msa0683300:CDS
AGTGAAAGCATCGCCGGAGA+AGG	0.534186	4_4:+45701682	None:intergenic
TCCTCGATCGTCAATGCAGA+CGG	0.536118	4_4:-45701161	Msa0683300:five_prime_UTR
GTCCCTTACACTATGCAAGC+TGG	0.537091	4_4:-45699396	Msa0683300:CDS
CAGCTATCCAAAACAATGGA+AGG	0.537219	4_4:-45699684	Msa0683300:CDS
TTATCGAAGGACTGGTAGAT+GGG	0.545730	4_4:-45700869	Msa0683300:CDS
GAGCGTCATTCTTTAAGGAA+TGG	0.549463	4_4:-45699047	Msa0683300:CDS
CGAGCTGCTATTAAGTTTAG+GGG	0.549687	4_4:-45700414	Msa0683300:CDS
GGCCGTTGGGAAGCTCGAAT+GGG	0.552095	4_4:-45700149	Msa0683300:CDS
GTGAAAGCATCGCCGGAGAA+GGG	0.556498	4_4:+45701683	None:intergenic
ACCTCATTGGAAAAGAAAGC+GGG	0.560515	4_4:+45699017	None:intergenic
TTTGGGACCAGGCCCTGGCG+TGG	0.562772	4_4:+45699452	None:intergenic
CCGGTTACTGGAAAAGTGAG+AGG	0.570605	4_4:+45701565	None:intergenic
CAAAGCAATGGTTTCTCAAG+AGG	0.570934	4_4:-45700209	Msa0683300:CDS
ATAGTGTAAGGGACAGATGA+GGG	0.572588	4_4:+45699405	None:intergenic
ACTCAGAGAACGAGGTTGAG+AGG	0.572966	4_4:+45701605	None:intergenic
ACACAGCTGACGCTGCTGCT+AGG	0.577273	4_4:-45700530	Msa0683300:intron
TTCCAGCTTGCATAGTGTAA+GGG	0.578047	4_4:+45699394	None:intergenic
AACTAACTTTCAACCGTCGC+CGG	0.582969	4_4:+45701377	None:intergenic
GATTGTTAATCCATTATCGC+CGG	0.585098	4_4:-45701417	Msa0683300:five_prime_UTR
GGCGATGAGATTAACGTCGC+CGG	0.586780	4_4:+45701655	None:intergenic
AATGAAGTCGGTAACTAACA+TGG	0.592484	4_4:+45698904	None:intergenic
TGAAAGCATCGCCGGAGAAG+GGG	0.594113	4_4:+45701684	None:intergenic
GGAGAAAGTGAAAGCATCGC+CGG	0.594178	4_4:+45701676	None:intergenic
AAAGAACTTTCCGCAAACAG+TGG	0.595682	4_4:-45701204	Msa0683300:five_prime_UTR
TTTCGATATTCTAAAATGGG+AGG	0.596951	4_4:-45701099	Msa0683300:exon
CATAGTGTAAGGGACAGATG+AGG	0.597697	4_4:+45699404	None:intergenic
GCAAGCTGGAAGAAGTTCAA+AGG	0.603351	4_4:-45699382	Msa0683300:intron
CACCTCATTGGAAAAGAAAG+CGG	0.604177	4_4:+45699016	None:intergenic
CTCACTTACACGATGACAAG+AGG	0.605140	4_4:-45698639	Msa0683300:three_prime_UTR
AAAGCAATGGTTTCTCAAGA+GGG	0.608305	4_4:-45700208	Msa0683300:CDS
ACGTAGCTTGCAACTTCACA+GGG	0.608726	4_4:+45701040	None:intergenic
ATACTTCGTCGACAAAGCAA+TGG	0.609369	4_4:-45700221	Msa0683300:CDS
GAAATCTGCAACCATCAATG+AGG	0.609375	4_4:-45699617	Msa0683300:CDS
TTGAGTTTCTCTGAGAACGC+CGG	0.609957	4_4:+45701533	None:intergenic
ACCGTCTGCATTGACGATCG+AGG	0.612606	4_4:+45701160	None:intergenic
AAAGCAGCTATCCAAAACAA+TGG	0.612644	4_4:-45699688	Msa0683300:CDS
GTCCCATTCGAGCTTCCCAA+CGG	0.614222	4_4:+45700147	None:intergenic
AAATCTGCAACCATCAATGA+GGG	0.619673	4_4:-45699616	Msa0683300:intron
AGCTATCCAAAACAATGGAA+GGG	0.627347	4_4:-45699683	Msa0683300:CDS
ACGAAAGTTCAATTGATCGT+CGG	0.634373	4_4:+45701634	None:intergenic
TATTGCGAACTACGAGACCT+AGG	0.636461	4_4:+45700907	None:intergenic
GGAGTAACACTGCACAAATG+TGG	0.639362	4_4:-45700170	Msa0683300:CDS
AGAAGAGTAGAAGAGGACCT+AGG	0.639611	4_4:-45700924	Msa0683300:CDS
CTGGTCCCAAAGAAAACTGG+GGG	0.643254	4_4:-45699440	Msa0683300:CDS
TTCAAACGAAGTCGTAACGA+AGG	0.645074	4_4:-45701070	Msa0683300:CDS
ATTTGGGCATAGCCACGCCA+GGG	0.650172	4_4:-45699464	Msa0683300:CDS
GTTACTGGAAAAGTGAGAGG+TGG	0.651492	4_4:+45701568	None:intergenic
TCACTTACACGATGACAAGA+GGG	0.652178	4_4:-45698638	Msa0683300:three_prime_UTR
ACTAACATGGATGATTGAGA+AGG	0.656872	4_4:+45698917	None:intergenic
TAGTGTAAGGGACAGATGAG+GGG	0.658256	4_4:+45699406	None:intergenic
ACACTGAAGTAGAAGCTGCA+AGG	0.668314	4_4:-45699837	Msa0683300:intron
AGAGGGAGTTCGAAGTACAG+AGG	0.669918	4_4:-45700191	Msa0683300:CDS
ACTCAAAGTGAAATCGCGCG+TGG	0.676094	4_4:+45701121	None:intergenic
AAACGGAAACTCAGAGAACG+AGG	0.688267	4_4:+45701597	None:intergenic
CCTGGTCCCAAAGAAAACTG+GGG	0.696186	4_4:-45699441	Msa0683300:CDS
TCTCGTAGTTCGCAATACAG+AGG	0.696706	4_4:-45700901	Msa0683300:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATTTTTGTAATTATTTGTA+GGG	-	chr4_4:45699577-45699596	Msa0683300:intron	10.0%
!!!	ATTATATGATTTACTATTTT+AGG	-	chr4_4:45699654-45699673	Msa0683300:CDS	10.0%
!!!	ATTTATATGAAGTTTGATTT+TGG	-	chr4_4:45699505-45699524	Msa0683300:intron	15.0%
!!!	GTATTTTTGTAATTATTTGT+AGG	-	chr4_4:45699576-45699595	Msa0683300:intron	15.0%
!!	ACTTCAGTTAAATTAAAAAT+AGG	+	chr4_4:45700161-45700180	None:intergenic	15.0%
!!!	TATTATGAATTTAAAACCTA+AGG	-	chr4_4:45700203-45700222	Msa0683300:CDS	15.0%
!!!	TAATTTTCAGAAAATCAAAA+GGG	+	chr4_4:45700540-45700559	None:intergenic	15.0%
!!!	TTTTGATTTTCTGAAAATTA+GGG	-	chr4_4:45700540-45700559	Msa0683300:CDS	15.0%
!!!	CAAAGATCTTATTTAAATTA+AGG	-	chr4_4:45700683-45700702	Msa0683300:intron	15.0%
!!!	CTAATTTTCAGAAAATCAAA+AGG	+	chr4_4:45700541-45700560	None:intergenic	20.0%
!!!	CTTTTGATTTTCTGAAAATT+AGG	-	chr4_4:45700539-45700558	Msa0683300:CDS	20.0%
!!!	AAAATGTTTTACAAAGATCA+AGG	+	chr4_4:45701057-45701076	None:intergenic	20.0%
!!!	TTCGATTCAATTGTTTTGAT+CGG	-	chr4_4:45698820-45698839	Msa0683300:three_prime_UTR	25.0%
!!	TGAGTTTCGATATTCTAAAA+TGG	-	chr4_4:45699156-45699175	Msa0683300:intron	25.0%
!!!	GAGTTTCGATATTCTAAAAT+GGG	-	chr4_4:45699157-45699176	Msa0683300:intron	25.0%
!!	AATTTGTGGTTGAATTTTGT+AGG	-	chr4_4:45699698-45699717	Msa0683300:CDS	25.0%
!	TTGTTGAATTTGAATTGACT+TGG	-	chr4_4:45699797-45699816	Msa0683300:intron	25.0%
!!!	TGAAACTAATTCACTTTTGT+TGG	+	chr4_4:45700232-45700251	None:intergenic	25.0%
!!	AATTAGTTTCAGCTTAAATC+TGG	-	chr4_4:45700241-45700260	Msa0683300:CDS	25.0%
!	GTAATAATTCATTAGTGCTT+AGG	+	chr4_4:45700449-45700468	None:intergenic	25.0%
!	AAGTGTAATAAATTTAGTGC+AGG	+	chr4_4:45700478-45700497	None:intergenic	25.0%
!!!	TTTTTATTTGCTTTCAAAGC+AGG	-	chr4_4:45700749-45700768	Msa0683300:intron	25.0%
!	TCAAAATCTTGATCTCAATT+TGG	-	chr4_4:45700778-45700797	Msa0683300:intron	25.0%
!	CAAAATCTTGATCTCAATTT+GGG	-	chr4_4:45700779-45700798	Msa0683300:intron	25.0%
!	TGTATTTGAGCTTTACATAT+AGG	-	chr4_4:45700902-45700921	Msa0683300:CDS	25.0%
!!	AAGAGCTTTTGTAATCATTT+TGG	-	chr4_4:45700991-45701010	Msa0683300:CDS	25.0%
!!	AGAGCTTTTGTAATCATTTT+GGG	-	chr4_4:45700992-45701011	Msa0683300:CDS	25.0%
!!!	TAGTTATTTAGTTTTACAGC+TGG	-	chr4_4:45701277-45701296	Msa0683300:five_prime_UTR	25.0%
!	GTCAATCATATTAATGAAGT+CGG	+	chr4_4:45701370-45701389	None:intergenic	25.0%
!!	TAATGAATTCTATGAGCTTT+TGG	-	chr4_4:45701445-45701464	Msa0683300:five_prime_UTR	25.0%
	GTTCTGATTCTGAATTGAAT+CGG	+	chr4_4:45699015-45699034	None:intergenic	30.0%
!!	TTTCGATATTCTAAAATGGG+AGG	-	chr4_4:45699160-45699179	Msa0683300:intron	30.0%
!	CATATCTGGTTCGTAAATTT+CGG	-	chr4_4:45699418-45699437	Msa0683300:CDS	30.0%
	GTAATTATTTGTAGGGATTG+CGG	-	chr4_4:45699584-45699603	Msa0683300:intron	30.0%
	TAATTATTTGTAGGGATTGC+GGG	-	chr4_4:45699585-45699604	Msa0683300:intron	30.0%
!	TGAGTTTTTGACGAAATTTG+TGG	-	chr4_4:45699684-45699703	Msa0683300:CDS	30.0%
	TTACGATGATGATCTTAAAC+AGG	-	chr4_4:45699901-45699920	Msa0683300:intron	30.0%
	TTGATGAATGTGAAACTAGA+TGG	-	chr4_4:45699945-45699964	Msa0683300:intron	30.0%
!!	AACTAATTCACTTTTGTTGG+AGG	+	chr4_4:45700229-45700248	None:intergenic	30.0%
!	TTAAATCTGGACACACTTTT+AGG	-	chr4_4:45700254-45700273	Msa0683300:CDS	30.0%
	TCTGAGAATTGAGAAAGAAT+TGG	-	chr4_4:45700329-45700348	Msa0683300:intron	30.0%
	AATTGAGAAAGAATTGGCAA+AGG	-	chr4_4:45700335-45700354	Msa0683300:intron	30.0%
	ACTTGATTATCAAGCTCATT+TGG	-	chr4_4:45700494-45700513	Msa0683300:intron	30.0%
	TATCTGTTATTGAGCAATCA+AGG	-	chr4_4:45701468-45701487	Msa0683300:five_prime_UTR	30.0%
	ACGAAAGTTCAATTGATCGT+CGG	+	chr4_4:45698628-45698647	None:intergenic	35.0%
	GATTGTTAATCCATTATCGC+CGG	-	chr4_4:45698842-45698861	Msa0683300:three_prime_UTR	35.0%
	TGCGGGAAACAAGTTTATTT+AGG	-	chr4_4:45699602-45699621	Msa0683300:intron	35.0%
!	TTGTGGTTGAATTTTGTAGG+TGG	-	chr4_4:45699701-45699720	Msa0683300:CDS	35.0%
	ATCGAGCTGCTATTAAGTTT+AGG	-	chr4_4:45699843-45699862	Msa0683300:CDS	35.0%
	TCGAGCTGCTATTAAGTTTA+GGG	-	chr4_4:45699844-45699863	Msa0683300:CDS	35.0%
	GACGAAGAATTTATCGAAAG+AGG	-	chr4_4:45700004-45700023	Msa0683300:intron	35.0%
	AAAGCAATGGTTTCTCAAGA+GGG	-	chr4_4:45700051-45700070	Msa0683300:intron	35.0%
!!!	AAGAATTGGCAAAGGTGTTT+TGG	-	chr4_4:45700343-45700362	Msa0683300:intron	35.0%
	TCGTGCAGGTATATATATCT+TGG	-	chr4_4:45700391-45700410	Msa0683300:CDS	35.0%
	CGTGCAGGTATATATATCTT+GGG	-	chr4_4:45700392-45700411	Msa0683300:CDS	35.0%
	ATCAAAAGGGAGATACATGT+TGG	+	chr4_4:45700527-45700546	None:intergenic	35.0%
	AAAGCAGCTATCCAAAACAA+TGG	-	chr4_4:45700571-45700590	Msa0683300:intron	35.0%
	AGCTATCCAAAACAATGGAA+GGG	-	chr4_4:45700576-45700595	Msa0683300:intron	35.0%
!	GGAAGCAATGACTAATTTTG+AGG	-	chr4_4:45700597-45700616	Msa0683300:intron	35.0%
	AAATCTGCAACCATCAATGA+GGG	-	chr4_4:45700643-45700662	Msa0683300:intron	35.0%
!	ATGTTTGTTACCCTCATTGA+TGG	+	chr4_4:45700656-45700675	None:intergenic	35.0%
	AATGTTCTCTCTGTGAAATC+TGG	-	chr4_4:45700726-45700745	Msa0683300:intron	35.0%
	TTATTTGCTTTCAAAGCAGG+TGG	-	chr4_4:45700752-45700771	Msa0683300:intron	35.0%
	ACAAAGATCAAGGACATCAA+CGG	+	chr4_4:45701047-45701066	None:intergenic	35.0%
	TCCTACTGAGTTGTCATAAT+TGG	-	chr4_4:45701108-45701127	Msa0683300:five_prime_UTR	35.0%
	GAAACCAATGTTTCAGGAAT+CGG	-	chr4_4:45701155-45701174	Msa0683300:five_prime_UTR	35.0%
!	GCTAATAGCCTTTTCAAAGA+TGG	+	chr4_4:45701185-45701204	None:intergenic	35.0%
	TACTTCTACTCAAGAGAAAC+AGG	+	chr4_4:45701312-45701331	None:intergenic	35.0%
	ACTAACATGGATGATTGAGA+AGG	+	chr4_4:45701345-45701364	None:intergenic	35.0%
	AATGAAGTCGGTAACTAACA+TGG	+	chr4_4:45701358-45701377	None:intergenic	35.0%
	CAGTAACCGGAAAAATTCTC+CGG	-	chr4_4:45698707-45698726	Msa0683300:three_prime_UTR	40.0%
!	GATGATTTTTCCGGCGATAA+TGG	+	chr4_4:45698855-45698874	None:intergenic	40.0%
!!	GGCGATGATGATGATTTTTC+CGG	+	chr4_4:45698864-45698883	None:intergenic	40.0%
	GGAAAAATCATCATCATCGC+CGG	-	chr4_4:45698863-45698882	Msa0683300:three_prime_UTR	40.0%
	AAAGAACTTTCCGCAAACAG+TGG	-	chr4_4:45699055-45699074	Msa0683300:CDS	40.0%
	TTCAAACGAAGTCGTAACGA+AGG	-	chr4_4:45699189-45699208	Msa0683300:intron	40.0%
!!	TCGTCGTTTTCGATGAAGAA+CGG	-	chr4_4:45699247-45699266	Msa0683300:intron	40.0%
!	GTTTTCGATGAAGAACGGTT+CGG	-	chr4_4:45699252-45699271	Msa0683300:intron	40.0%
!	CTACTCTTCTTCACTTGTTG+TGG	+	chr4_4:45699325-45699344	None:intergenic	40.0%
	CAAGTGAAGAAGAGTAGAAG+AGG	-	chr4_4:45699328-45699347	Msa0683300:intron	40.0%
!!	GAGGTGTTACGTTTTATCGA+AGG	-	chr4_4:45699377-45699396	Msa0683300:intron	40.0%
!	GTTACGTTTTATCGAAGGAC+TGG	-	chr4_4:45699382-45699401	Msa0683300:intron	40.0%
!	TTTATCGAAGGACTGGTAGA+TGG	-	chr4_4:45699389-45699408	Msa0683300:CDS	40.0%
!	TTATCGAAGGACTGGTAGAT+GGG	-	chr4_4:45699390-45699409	Msa0683300:CDS	40.0%
	CGAGCTGCTATTAAGTTTAG+GGG	-	chr4_4:45699845-45699864	Msa0683300:CDS	40.0%
	GCTATTAAGTTTAGGGGAGT+TGG	-	chr4_4:45699851-45699870	Msa0683300:CDS	40.0%
	ATACTTCGTCGACAAAGCAA+TGG	-	chr4_4:45700038-45700057	Msa0683300:intron	40.0%
	CAAAGCAATGGTTTCTCAAG+AGG	-	chr4_4:45700050-45700069	Msa0683300:intron	40.0%
!!!	TAGGTTCTCACTTTTTGCCA+AGG	+	chr4_4:45700142-45700161	None:intergenic	40.0%
!!!	TTGGCAAAGGTGTTTTGGTT+TGG	-	chr4_4:45700348-45700367	Msa0683300:intron	40.0%
	CAGCTATCCAAAACAATGGA+AGG	-	chr4_4:45700575-45700594	Msa0683300:intron	40.0%
!	TTGCTTCCCTTCCATTGTTT+TGG	+	chr4_4:45700585-45700604	None:intergenic	40.0%
!	TTTGAGGCCAGTACATATGA+AGG	-	chr4_4:45700613-45700632	Msa0683300:intron	40.0%
	CATCTCTCCTTCATATGTAC+TGG	+	chr4_4:45700623-45700642	None:intergenic	40.0%
	GAAATCTGCAACCATCAATG+AGG	-	chr4_4:45700642-45700661	Msa0683300:intron	40.0%
	ATAGTGTAAGGGACAGATGA+GGG	+	chr4_4:45700857-45700876	None:intergenic	40.0%
	TTCCAGCTTGCATAGTGTAA+GGG	+	chr4_4:45700868-45700887	None:intergenic	40.0%
	TTTCTTCTGCTTTATGCCGA+AGG	-	chr4_4:45700925-45700944	Msa0683300:CDS	40.0%
	CCACTATTACCATCAACCTT+CGG	+	chr4_4:45700944-45700963	None:intergenic	40.0%
	GCCAATTATGACAACTCAGT+AGG	+	chr4_4:45701112-45701131	None:intergenic	40.0%
	GAGTTCTCTTCATTTGCAGA+TGG	-	chr4_4:45701133-45701152	Msa0683300:five_prime_UTR	40.0%
	CAGATGGAAACCAATGTTTC+AGG	-	chr4_4:45701149-45701168	Msa0683300:five_prime_UTR	40.0%
!!	ATCACCGATTCCTGAAACAT+TGG	+	chr4_4:45701162-45701181	None:intergenic	40.0%
!	TCGGTGATCCATCTTTGAAA+AGG	-	chr4_4:45701174-45701193	Msa0683300:five_prime_UTR	40.0%
	CCTTAAAGAATGACGCTCTT+CGG	+	chr4_4:45701210-45701229	None:intergenic	40.0%
!	GAGCGTCATTCTTTAAGGAA+TGG	-	chr4_4:45701212-45701231	Msa0683300:five_prime_UTR	40.0%
	ACCTCATTGGAAAAGAAAGC+GGG	+	chr4_4:45701245-45701264	None:intergenic	40.0%
	CACCTCATTGGAAAAGAAAG+CGG	+	chr4_4:45701246-45701265	None:intergenic	40.0%
	AGATGACGAAATCACCTCAT+TGG	+	chr4_4:45701258-45701277	None:intergenic	40.0%
	TCACTTACACGATGACAAGA+GGG	-	chr4_4:45701621-45701640	Msa0683300:five_prime_UTR	40.0%
	ACAAGAGGGTAACGTGTAAT+TGG	-	chr4_4:45701635-45701654	Msa0683300:five_prime_UTR	40.0%
	AAACGGAAACTCAGAGAACG+AGG	+	chr4_4:45698665-45698684	None:intergenic	45.0%
	GTTACTGGAAAAGTGAGAGG+TGG	+	chr4_4:45698694-45698713	None:intergenic	45.0%
!!	CCTCTCACTTTTCCAGTAAC+CGG	-	chr4_4:45698694-45698713	Msa0683300:three_prime_UTR	45.0%
!	CGGAGAATTTTTCCGGTTAC+TGG	+	chr4_4:45698709-45698728	None:intergenic	45.0%
!	AGAACGCCGGAGAATTTTTC+CGG	+	chr4_4:45698716-45698735	None:intergenic	45.0%
!	TTGAGTTTCTCTGAGAACGC+CGG	+	chr4_4:45698729-45698748	None:intergenic	45.0%
	AACTAACTTTCAACCGTCGC+CGG	+	chr4_4:45698885-45698904	None:intergenic	45.0%
	CCTGATTCATCCACTGTTTG+CGG	+	chr4_4:45699068-45699087	None:intergenic	45.0%
!	ACGTAGCTTGCAACTTCACA+GGG	+	chr4_4:45699222-45699241	None:intergenic	45.0%
	AGAAGAGTAGAAGAGGACCT+AGG	-	chr4_4:45699335-45699354	Msa0683300:intron	45.0%
	TATTGCGAACTACGAGACCT+AGG	+	chr4_4:45699355-45699374	None:intergenic	45.0%
	TCTCGTAGTTCGCAATACAG+AGG	-	chr4_4:45699358-45699377	Msa0683300:intron	45.0%
	GTAGATGGGAATCGCATATC+TGG	-	chr4_4:45699404-45699423	Msa0683300:CDS	45.0%
	GGAGTAACACTGCACAAATG+TGG	-	chr4_4:45700089-45700108	Msa0683300:intron	45.0%
!!	TCACTTTTGTTGGAGGCCTT+AGG	+	chr4_4:45700222-45700241	None:intergenic	45.0%
	ACACTGAAGTAGAAGCTGCA+AGG	-	chr4_4:45700422-45700441	Msa0683300:CDS	45.0%
!	AGTTGCCCCCAGTTTTCTTT+GGG	+	chr4_4:45700827-45700846	None:intergenic	45.0%
!	AAGTTGCCCCCAGTTTTCTT+TGG	+	chr4_4:45700828-45700847	None:intergenic	45.0%
	TAGTGTAAGGGACAGATGAG+GGG	+	chr4_4:45700856-45700875	None:intergenic	45.0%
	CATAGTGTAAGGGACAGATG+AGG	+	chr4_4:45700858-45700877	None:intergenic	45.0%
	CTTCCAGCTTGCATAGTGTA+AGG	+	chr4_4:45700869-45700888	None:intergenic	45.0%
	GCAAGCTGGAAGAAGTTCAA+AGG	-	chr4_4:45700877-45700896	Msa0683300:CDS	45.0%
	TGCTTTATGCCGAAGGTTGA+TGG	-	chr4_4:45700932-45700951	Msa0683300:CDS	45.0%
!!	CCGAAGGTTGATGGTAATAG+TGG	-	chr4_4:45700941-45700960	Msa0683300:CDS	45.0%
	CACTTGTAGCTAGTTGCAAC+CGG	-	chr4_4:45701017-45701036	Msa0683300:CDS	45.0%
	CAAGGACATCAACGGAAAAC+CGG	+	chr4_4:45701039-45701058	None:intergenic	45.0%
!!	CCGAAGAGCGTCATTCTTTA+AGG	-	chr4_4:45701207-45701226	Msa0683300:five_prime_UTR	45.0%
!	TCCCGCTTTCTTTTCCAATG+AGG	-	chr4_4:45701241-45701260	Msa0683300:five_prime_UTR	45.0%
	CTCACTTACACGATGACAAG+AGG	-	chr4_4:45701620-45701639	Msa0683300:five_prime_UTR	45.0%
!!	GGAGAAAGTGAAAGCATCGC+CGG	+	chr4_4:45698586-45698605	None:intergenic	50.0%
	GGCGATGCTTTCACTTTCTC+CGG	-	chr4_4:45698585-45698604	Msa0683300:three_prime_UTR	50.0%
	CTCAGAGAACGAGGTTGAGA+GGG	+	chr4_4:45698656-45698675	None:intergenic	50.0%
	ACTCAGAGAACGAGGTTGAG+AGG	+	chr4_4:45698657-45698676	None:intergenic	50.0%
	GTGAGAGGTGGAGGAAGAAA+CGG	+	chr4_4:45698682-45698701	None:intergenic	50.0%
	ACTGGAAAAGTGAGAGGTGG+AGG	+	chr4_4:45698691-45698710	None:intergenic	50.0%
	CCGGTTACTGGAAAAGTGAG+AGG	+	chr4_4:45698697-45698716	None:intergenic	50.0%
	CCGCAAACAGTGGATGAATC+AGG	-	chr4_4:45699065-45699084	Msa0683300:CDS	50.0%
	TCCTCGATCGTCAATGCAGA+CGG	-	chr4_4:45699098-45699117	Msa0683300:CDS	50.0%
	ACTCAAAGTGAAATCGCGCG+TGG	+	chr4_4:45699141-45699160	None:intergenic	50.0%
!	GACGTAGCTTGCAACTTCAC+AGG	+	chr4_4:45699223-45699242	None:intergenic	50.0%
	AGAGGGAGTTCGAAGTACAG+AGG	-	chr4_4:45700068-45700087	Msa0683300:intron	50.0%
	ACTGCACAAATGTGGCCGTT+GGG	-	chr4_4:45700097-45700116	Msa0683300:intron	50.0%
	GCTCGAATGGGACAATTCCT+TGG	-	chr4_4:45700122-45700141	Msa0683300:intron	50.0%
	AGATTGCGATGCTTTCGTGC+AGG	-	chr4_4:45700377-45700396	Msa0683300:CDS	50.0%
	GCCTGGTCCCAAAGAAAACT+GGG	-	chr4_4:45700817-45700836	Msa0683300:intron	50.0%
	CCTGGTCCCAAAGAAAACTG+GGG	-	chr4_4:45700818-45700837	Msa0683300:intron	50.0%
	CTGGTCCCAAAGAAAACTGG+GGG	-	chr4_4:45700819-45700838	Msa0683300:intron	50.0%
	GTCCCTTACACTATGCAAGC+TGG	-	chr4_4:45700863-45700882	Msa0683300:CDS	50.0%
	TCTCTCTCTCTCCCCTTCTC+CGG	-	chr4_4:45698564-45698583	Msa0683300:three_prime_UTR	55.0%
!!	TGAAAGCATCGCCGGAGAAG+GGG	+	chr4_4:45698578-45698597	None:intergenic	55.0%
!!	GTGAAAGCATCGCCGGAGAA+GGG	+	chr4_4:45698579-45698598	None:intergenic	55.0%
!!	AGTGAAAGCATCGCCGGAGA+AGG	+	chr4_4:45698580-45698599	None:intergenic	55.0%
	GGCGATGAGATTAACGTCGC+CGG	+	chr4_4:45698607-45698626	None:intergenic	55.0%
	ATCATCATCATCGCCGGCGA+CGG	-	chr4_4:45698869-45698888	Msa0683300:three_prime_UTR	55.0%
!	ACCGTCTGCATTGACGATCG+AGG	+	chr4_4:45699102-45699121	None:intergenic	55.0%
	CACTGCACAAATGTGGCCGT+TGG	-	chr4_4:45700096-45700115	Msa0683300:intron	55.0%
	GTCCCATTCGAGCTTCCCAA+CGG	+	chr4_4:45700115-45700134	None:intergenic	55.0%
	AATTTGGGCATAGCCACGCC+AGG	-	chr4_4:45700794-45700813	Msa0683300:intron	55.0%
	ATTTGGGCATAGCCACGCCA+GGG	-	chr4_4:45700795-45700814	Msa0683300:intron	55.0%
!!!	TTTTCTTTGGGACCAGGCCC+TGG	+	chr4_4:45700815-45700834	None:intergenic	55.0%
	GGCCTGGTCCCAAAGAAAAC+TGG	-	chr4_4:45700816-45700835	Msa0683300:intron	55.0%
!!!	CCCCAGTTTTCTTTGGGACC+AGG	+	chr4_4:45700821-45700840	None:intergenic	55.0%
	ACACAGCTGACGCTGCTGCT+AGG	-	chr4_4:45699729-45699748	Msa0683300:intron	60.0%
	TGGCCGTTGGGAAGCTCGAA+TGG	-	chr4_4:45700109-45700128	Msa0683300:intron	60.0%
	GGCCGTTGGGAAGCTCGAAT+GGG	-	chr4_4:45700110-45700129	Msa0683300:intron	60.0%
!	TTTGGGACCAGGCCCTGGCG+TGG	+	chr4_4:45700810-45700829	None:intergenic	70.0%
	GGCATAGCCACGCCAGGGCC+TGG	-	chr4_4:45700800-45700819	Msa0683300:intron	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	45698557	45701724	45698557	ID=Msa0683300;Name=Msa0683300
chr4_4	mRNA	45698557	45701724	45698557	ID=Msa0683300-mRNA-1;Parent=Msa0683300;Name=Msa0683300-mRNA-1;_AED=0.16;_eAED=0.16;_QI=618\|0.75\|0.88\|1\|1\|1\|9\|402\|329
chr4_4	exon	45698557	45699129	45698557	ID=Msa0683300-mRNA-1:exon:11215;Parent=Msa0683300-mRNA-1
chr4_4	exon	45699383	45699510	45699383	ID=Msa0683300-mRNA-1:exon:11214;Parent=Msa0683300-mRNA-1
chr4_4	exon	45699617	45699720	45699617	ID=Msa0683300-mRNA-1:exon:11213;Parent=Msa0683300-mRNA-1
chr4_4	exon	45699838	45699882	45699838	ID=Msa0683300-mRNA-1:exon:11212;Parent=Msa0683300-mRNA-1
chr4_4	exon	45700131	45700276	45700131	ID=Msa0683300-mRNA-1:exon:11211;Parent=Msa0683300-mRNA-1
chr4_4	exon	45700359	45700446	45700359	ID=Msa0683300-mRNA-1:exon:11210;Parent=Msa0683300-mRNA-1
chr4_4	exon	45700531	45700561	45700531	ID=Msa0683300-mRNA-1:exon:11209;Parent=Msa0683300-mRNA-1
chr4_4	exon	45700658	45700683	45700658	ID=Msa0683300-mRNA-1:exon:11208;Parent=Msa0683300-mRNA-1
chr4_4	exon	45700856	45701724	45700856	ID=Msa0683300-mRNA-1:exon:11207;Parent=Msa0683300-mRNA-1
chr4_4	five_prime_UTR	45701107	45701724	45701107	ID=Msa0683300-mRNA-1:five_prime_utr;Parent=Msa0683300-mRNA-1
chr4_4	CDS	45700856	45701106	45700856	ID=Msa0683300-mRNA-1:cds;Parent=Msa0683300-mRNA-1
chr4_4	CDS	45700658	45700683	45700658	ID=Msa0683300-mRNA-1:cds;Parent=Msa0683300-mRNA-1
chr4_4	CDS	45700531	45700561	45700531	ID=Msa0683300-mRNA-1:cds;Parent=Msa0683300-mRNA-1
chr4_4	CDS	45700359	45700446	45700359	ID=Msa0683300-mRNA-1:cds;Parent=Msa0683300-mRNA-1
chr4_4	CDS	45700131	45700276	45700131	ID=Msa0683300-mRNA-1:cds;Parent=Msa0683300-mRNA-1
chr4_4	CDS	45699838	45699882	45699838	ID=Msa0683300-mRNA-1:cds;Parent=Msa0683300-mRNA-1
chr4_4	CDS	45699617	45699720	45699617	ID=Msa0683300-mRNA-1:cds;Parent=Msa0683300-mRNA-1
chr4_4	CDS	45699383	45699510	45699383	ID=Msa0683300-mRNA-1:cds;Parent=Msa0683300-mRNA-1
chr4_4	CDS	45698959	45699129	45698959	ID=Msa0683300-mRNA-1:cds;Parent=Msa0683300-mRNA-1
chr4_4	three_prime_UTR	45698557	45698958	45698557	ID=Msa0683300-mRNA-1:three_prime_utr;Parent=Msa0683300-mRNA-1

Gene Sequence

>Msa0683300

ATGGGAGGTTCAAATTCAAACGAAGTCGTAACGAAGGAGTTTTTCCCTGTGAAGTTGCAAGCTACGTCGTCGTCGTCGTTTTCGATGAAGAACGGTTCGGTTGATTTGTCGTTTAATCAAAACGAAAAGATGAAGATTGTTCAAGCGCCACAACAAGTGAAGAAGAGTAGAAGAGGACCTAGGTCTCGTAGTTCGCAATACAGAGGTGTTACGTTTTATCGAAGGACTGGTAGATGGGAATCGCATATCTGGGATTGCGGGAAACAAGTTTATTTAGGTGGATTTGACACAGCTGACGCTGCTGCTAGAGCTTATGATCGAGCTGCTATTAAGTTTAGGGGAGTTGGTGCTGACATAAATTTCAATTTGAATGATTACGATGATGATCTTAAACAGACGAAGAATTTATCGAAAGAGGAGTTCGTGCAAATACTTCGTCGACAAAGCAATGGTTTCTCAAGAGGGAGTTCGAAGTACAGAGGAGTAACACTGCACAAATGTGGCCGTTGGGAAGCTCGAATGGGACAATTCCTTGGCAAAAAGTATATATATCTTGGGTTATTCGACACTGAAGTAGAAGCTGCAAGGGCTTATGACAAAGCAGCTATCCAAAACAATGGAAGGGAAGCAATGACTAATTTTGAGGCCAGTACATATGAAGGAGAGATGAAATCTGCAACCATCAATGAGGGTGGCAGTCAAAATCTTGATCTCAATTTGGGCATAGCCACGCCAGGGCCTGGTCCCAAAGAAAACTGGGGGCAACTTCACTTCCCCTCATCTGTCCCTTACACTATGCAAGCTGGAAGAAGTTCAAAGATGGAAACCAATGTTTCAGGAATCGGTGATCCATCTTTGAAAAGGCTATTAGCAACCGAAGAGCGTCATTCTTTAAGGAATGGCATGTATCCCGCTTTCTTTTCCAATGAGGTGATTTCGTCATCTAGTTATTTAGTTTTACAGCTGGAGTCTATTGCCTGTTTCTCTTGA

Protein sequence

>Msa0683300

MGGSNSNEVVTKEFFPVKLQATSSSSFSMKNGSVDLSFNQNEKMKIVQAPQQVKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTADAAARAYDRAAIKFRGVGADINFNLNDYDDDLKQTKNLSKEEFVQILRRQSNGFSRGSSKYRGVTLHKCGRWEARMGQFLGKKYIYLGLFDTEVEAARAYDKAAIQNNGREAMTNFEASTYEGEMKSATINEGGSQNLDLNLGIATPGPGPKENWGQLHFPSSVPYTMQAGRSSKMETNVSGIGDPSLKRLLATEERHSLRNGMYPAFFSNEVISSSSYLVLQLESIACFS*