AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0691880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0691880 MtrunA17_Chr7g0271481 92.941 85 6 0 46 130 586 670 3.24e-49 168
Msa0691880 MtrunA17_Chr1g0210851 36.585 82 52 0 49 130 686 767 3.83e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0691880 AT3G07100.1 76.471 85 20 0 46 130 604 688 1.13e-39 141

Find 29 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCATAAAATGGATCTTC+AGG 0.293277 4_4:-60191562 None:intergenic
AGAAATTGAGTCATGAATTA+AGG 0.308061 4_4:+60192128 Msa0691880:CDS
CATACACATTTACTGAGATT+TGG 0.310882 4_4:-60191609 None:intergenic
GCAGCCATCTGCTCATAAAA+TGG 0.311495 4_4:-60191571 None:intergenic
CGTTGCAAGGATTGAACATA+AGG 0.423711 4_4:+60189106 Msa0691880:CDS
TCCTGTCTGTGTAGGAACTC+TGG 0.429146 4_4:+60192045 Msa0691880:intron
TCTCCCCATGAGTAGCTGAT+TGG 0.442822 4_4:-60192108 None:intergenic
CGTTGCAAGGATTGAACATA+AGG 0.444695 4_4:+60189132 Msa0691880:CDS
TGAGTAGCTGATTGGAAAGC+TGG 0.469035 4_4:-60192100 None:intergenic
AGTGAGGAAGATGATGGTTG+TGG 0.510469 4_4:-60189051 None:intergenic
GAGAGACTGCATGGGAAGCA+TGG 0.524555 4_4:+60192164 Msa0691880:CDS
GCTTTCCAATCAGCTACTCA+TGG 0.528786 4_4:+60192103 Msa0691880:CDS
TACACTGACATAGCCTCCTT+AGG 0.536987 4_4:+60191646 Msa0691880:CDS
CTTTCCAATCAGCTACTCAT+GGG 0.542155 4_4:+60192104 Msa0691880:CDS
GGAAAATAAAACACCTAAGG+AGG 0.554451 4_4:-60191659 None:intergenic
GAACATAAGGCATCGTTGCA+AGG 0.557616 4_4:+60189119 Msa0691880:CDS
ACCTCACAAGAGAGACTGCA+TGG 0.560035 4_4:+60192155 Msa0691880:CDS
ATCAACGACGATGGAATCTG+AGG 0.564385 4_4:-60189080 None:intergenic
ACTCTGGCAAAGTACACTGC+TGG 0.591069 4_4:+60192061 Msa0691880:CDS
CGTCGTTGATCATCGTTGCA+AGG 0.594152 4_4:+60189093 Msa0691880:CDS
CCCATGCAGTCTCTCTTGTG+AGG 0.598716 4_4:-60192156 None:intergenic
CAAGGATTGAACATAAGGAA+AGG 0.607853 4_4:+60189137 Msa0691880:CDS
TTTGGGACAGATAAAGAACA+TGG 0.635621 4_4:+60191529 Msa0691880:intron
TCAACGACGATGGAATCTGA+GGG 0.637587 4_4:-60189079 None:intergenic
GCAACGATGATCAACGACGA+TGG 0.649323 4_4:-60189089 None:intergenic
TTAGCGGGAAGAAGAAGACA+TGG 0.669986 4_4:-60189026 None:intergenic
TTTCCAATCAGCTACTCATG+GGG 0.686768 4_4:+60192105 Msa0691880:CDS
CCTCACAAGAGAGACTGCAT+GGG 0.693489 4_4:+60192156 Msa0691880:CDS
GCGGGAAGAAGAAGACATGG+AGG 0.714817 4_4:-60189023 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCTTTTTTCAAATTATAT+AGG - chr4_4:60190188-60190207 None:intergenic 10.0%
!! TATTAGAAATAAGAAGATAT+GGG - chr4_4:60189291-60189310 None:intergenic 15.0%
!! ATATTAGAAATAAGAAGATA+TGG - chr4_4:60189292-60189311 None:intergenic 15.0%
!!! ATATCTTCTTATTTCTAATA+TGG + chr4_4:60189291-60189310 Msa0691880:intron 15.0%
!!! GAATATTTCATTTTACTTTA+GGG + chr4_4:60189313-60189332 Msa0691880:intron 15.0%
!!! ACTTTAGTAATTTTATTTAG+AGG - chr4_4:60189697-60189716 None:intergenic 15.0%
!!! TTCTAATTCAACAATTATTT+TGG - chr4_4:60190612-60190631 None:intergenic 15.0%
!!! ATGAAGAAATTAATCATTTT+AGG + chr4_4:60190639-60190658 Msa0691880:intron 15.0%
!!! TGAAGAAATTAATCATTTTA+GGG + chr4_4:60190640-60190659 Msa0691880:intron 15.0%
!!! GGAATATTTCATTTTACTTT+AGG + chr4_4:60189312-60189331 Msa0691880:intron 20.0%
!!! CTCTATATTGTTACTATTTT+TGG + chr4_4:60189412-60189431 Msa0691880:intron 20.0%
!! AAATTACTAAAGTGTATATG+TGG + chr4_4:60189704-60189723 Msa0691880:intron 20.0%
!!! TTAGAGATTTTACATGATTT+TGG + chr4_4:60189729-60189748 Msa0691880:intron 20.0%
!!! GCTCAATTTTTTTATAAGTA+CGG + chr4_4:60190119-60190138 Msa0691880:intron 20.0%
!! TTTATCTCAAATCTTGTATT+TGG + chr4_4:60190535-60190554 Msa0691880:intron 20.0%
!!! CAAATCTTTTCATTAAGAAT+TGG - chr4_4:60191032-60191051 None:intergenic 20.0%
!! ATTCTTAATGAAAAGATTTG+TGG + chr4_4:60191032-60191051 Msa0691880:intron 20.0%
!! TTAAAGTAATTAGAAATCCA+TGG + chr4_4:60191152-60191171 Msa0691880:intron 20.0%
!!! ATATTTTTGTTGTATTAGTG+TGG + chr4_4:60191320-60191339 Msa0691880:intron 20.0%
!! TAACAGGTTTAAAAATAGAA+TGG - chr4_4:60191710-60191729 None:intergenic 20.0%
!!! TTTAGGGTTTTCGTATTATA+TGG + chr4_4:60189329-60189348 Msa0691880:intron 25.0%
! TTCGTTTATTATTCTTTCCA+TGG + chr4_4:60189355-60189374 Msa0691880:intron 25.0%
!!! CATGTCTAATTATTTTGTGT+TGG + chr4_4:60189752-60189771 Msa0691880:intron 25.0%
! TTTCACTTGACATGAAATTT+AGG - chr4_4:60189791-60189810 None:intergenic 25.0%
!! GTGTCTCAAAATATTTTGTT+AGG + chr4_4:60189811-60189830 Msa0691880:intron 25.0%
! TCGAACTTGAAAATTAAATG+AGG + chr4_4:60189877-60189896 Msa0691880:intron 25.0%
!! CTAAGAAGTGCATAAAAATA+AGG + chr4_4:60190040-60190059 Msa0691880:intron 25.0%
! AAGGCAAAACAAAATGATAA+AGG + chr4_4:60190059-60190078 Msa0691880:intron 25.0%
! AGGCAAAACAAAATGATAAA+GGG + chr4_4:60190060-60190079 Msa0691880:intron 25.0%
!!! TACTGATTTAGTGAGAATTT+TGG + chr4_4:60190668-60190687 Msa0691880:intron 25.0%
!!! ACTGATTTAGTGAGAATTTT+GGG + chr4_4:60190669-60190688 Msa0691880:intron 25.0%
! ATTTGTCAGTAATTGCAAAT+GGG + chr4_4:60190825-60190844 Msa0691880:intron 25.0%
!!! AAGTTAAGTCATGTTTTAGT+TGG + chr4_4:60190873-60190892 Msa0691880:intron 25.0%
!!! GAAATTTTGGAAAAAATGTG+TGG + chr4_4:60191085-60191104 Msa0691880:intron 25.0%
! TTGACTTTAATAAGATGCTT+TGG + chr4_4:60191215-60191234 Msa0691880:intron 25.0%
! AAAACTAGTCATTTGTTCAT+TGG + chr4_4:60191297-60191316 Msa0691880:intron 25.0%
! AAAATTCATATGCAATGGTA+AGG - chr4_4:60191370-60191389 None:intergenic 25.0%
! GTGATAAAATTCATATGCAA+TGG - chr4_4:60191375-60191394 None:intergenic 25.0%
! ATGTTCTGATATTTGTGTAA+TGG + chr4_4:60191421-60191440 Msa0691880:intron 25.0%
! AAGTTCAACACACAAAAATA+TGG - chr4_4:60191489-60191508 None:intergenic 25.0%
!!! AGTGTATTAGTGTATTGATT+TGG + chr4_4:60191511-60191530 Msa0691880:intron 25.0%
!!! GTGTATTAGTGTATTGATTT+GGG + chr4_4:60191512-60191531 Msa0691880:intron 25.0%
! ACGAAATACATAGAAATTGA+AGG - chr4_4:60191683-60191702 None:intergenic 25.0%
! ATCAATTAATCAAGAGTAAC+AGG - chr4_4:60191726-60191745 None:intergenic 25.0%
! AGAAATTGAGTCATGAATTA+AGG + chr4_4:60192128-60192147 Msa0691880:CDS 25.0%
!!! ATTTTTGGCACTTTTGTTGT+TGG + chr4_4:60189427-60189446 Msa0691880:intron 30.0%
GAGGGACATAATTTATTCTT+TGG - chr4_4:60189956-60189975 None:intergenic 30.0%
GTTAAGAAGTTCATAGTTGA+GGG - chr4_4:60189974-60189993 None:intergenic 30.0%
TTCAAATTATATAGGCCACT+TGG - chr4_4:60190180-60190199 None:intergenic 30.0%
! TTTGGTAGTTTCATGCAATA+AGG + chr4_4:60190265-60190284 Msa0691880:intron 30.0%
! TTGGTAGTTTCATGCAATAA+GGG + chr4_4:60190266-60190285 Msa0691880:intron 30.0%
! TCAAATCTTGTATTTGGTGA+AGG + chr4_4:60190541-60190560 Msa0691880:intron 30.0%
! ACTGTCTAATTGTTGCATTT+TGG + chr4_4:60190762-60190781 Msa0691880:intron 30.0%
CAACGACATAACATCTTAAT+CGG - chr4_4:60190795-60190814 None:intergenic 30.0%
GATTTGTCAGTAATTGCAAA+TGG + chr4_4:60190824-60190843 Msa0691880:intron 30.0%
TTTGTCAGTAATTGCAAATG+GGG + chr4_4:60190826-60190845 Msa0691880:intron 30.0%
GTCACACTTATGTCAAAAAA+TGG + chr4_4:60191054-60191073 Msa0691880:intron 30.0%
!! AATGGACAGTGTTGAAATTT+TGG + chr4_4:60191072-60191091 Msa0691880:intron 30.0%
!! GATATTTGTGTAATGGATAG+AGG + chr4_4:60191428-60191447 Msa0691880:intron 30.0%
CATACACATTTACTGAGATT+TGG - chr4_4:60191612-60191631 None:intergenic 30.0%
GAAGGAAAATAAAACACCTA+AGG - chr4_4:60191665-60191684 None:intergenic 30.0%
! ATTTGATGATTCTTCAGTTG+TGG + chr4_4:60191748-60191767 Msa0691880:intron 30.0%
! ACTTGATGACATGAGTTTAT+GGG + chr4_4:60191786-60191805 Msa0691880:intron 30.0%
TAAACTGATACTCTAACTCA+TGG + chr4_4:60191829-60191848 Msa0691880:intron 30.0%
AAATGCTGACAGAATTACAT+CGG - chr4_4:60191959-60191978 None:intergenic 30.0%
!!! GTTTTTAGTGAGGAAGATGA+TGG - chr4_4:60189060-60189079 None:intergenic 35.0%
CAAGGATTGAACATAAGGAA+AGG + chr4_4:60189137-60189156 Msa0691880:CDS 35.0%
GGAAAGGTAACAATCTGATT+AGG + chr4_4:60189153-60189172 Msa0691880:intron 35.0%
GGTTAAGAAGTTCATAGTTG+AGG - chr4_4:60189975-60189994 None:intergenic 35.0%
! TCACTATACACTCTTTGAAG+TGG - chr4_4:60189996-60190015 None:intergenic 35.0%
! TTATATAGGCCACTTGGTTT+TGG - chr4_4:60190174-60190193 None:intergenic 35.0%
! ACAAAGGGGTGTATAGAAAA+GGG - chr4_4:60190220-60190239 None:intergenic 35.0%
! TACAAAGGGGTGTATAGAAA+AGG - chr4_4:60190221-60190240 None:intergenic 35.0%
TGGTAGTTTCATGCAATAAG+GGG + chr4_4:60190267-60190286 Msa0691880:intron 35.0%
GAGATAAACATCACCTTCAA+CGG - chr4_4:60190523-60190542 None:intergenic 35.0%
! GATGGGATGATAATTTGTGT+TGG + chr4_4:60190848-60190867 Msa0691880:intron 35.0%
AAGAGAGACTAAATGATTGG+AGG - chr4_4:60190975-60190994 None:intergenic 35.0%
CCAAAGAGAGACTAAATGAT+TGG - chr4_4:60190978-60190997 None:intergenic 35.0%
CCAATCATTTAGTCTCTCTT+TGG + chr4_4:60190975-60190994 Msa0691880:intron 35.0%
GTGGTTACATCTTTGTTACA+TGG + chr4_4:60191104-60191123 Msa0691880:intron 35.0%
TAACATCACACATTTCACCA+TGG - chr4_4:60191172-60191191 None:intergenic 35.0%
! TTTGGGACAGATAAAGAACA+TGG + chr4_4:60191529-60191548 Msa0691880:intron 35.0%
TGCTCATAAAATGGATCTTC+AGG - chr4_4:60191565-60191584 None:intergenic 35.0%
! AGATCCATTTTATGAGCAGA+TGG + chr4_4:60191567-60191586 Msa0691880:CDS 35.0%
GGAAAATAAAACACCTAAGG+AGG - chr4_4:60191662-60191681 None:intergenic 35.0%
! GACTTGATGACATGAGTTTA+TGG + chr4_4:60191785-60191804 Msa0691880:intron 35.0%
GGATCTATTTGACTATGAAC+TGG + chr4_4:60191850-60191869 Msa0691880:intron 35.0%
AATGCTGACAGAATTACATC+GGG - chr4_4:60191958-60191977 None:intergenic 35.0%
TGTAATTCTGTCAGCATTTG+CGG + chr4_4:60191960-60191979 Msa0691880:intron 35.0%
!!! GTTTTTTTTCCTGTCTGTGT+AGG + chr4_4:60192037-60192056 Msa0691880:intron 35.0%
!!! ATGATGGTTGTGGTTTTAGC+GGG - chr4_4:60189044-60189063 None:intergenic 40.0%
!!! GATGATGGTTGTGGTTTTAG+CGG - chr4_4:60189045-60189064 None:intergenic 40.0%
!!! GAATCTGAGGGTTTTTAGTG+AGG - chr4_4:60189070-60189089 None:intergenic 40.0%
CGTTGCAAGGATTGAACATA+AGG + chr4_4:60189106-60189125 Msa0691880:CDS 40.0%
CGTTGCAAGGATTGAACATA+AGG + chr4_4:60189106-60189125 Msa0691880:CDS 40.0%
! CGTTTTTCACTATCGTCGTT+AGG + chr4_4:60189192-60189211 Msa0691880:intron 40.0%
CTCATGTTCATCAGTATCCA+TGG - chr4_4:60189375-60189394 None:intergenic 40.0%
!!! GCACTTTTGTTGTTGGAACT+TGG + chr4_4:60189434-60189453 Msa0691880:intron 40.0%
!! ATACTCTGCGTTTTTGAGCT+TGG + chr4_4:60189515-60189534 Msa0691880:intron 40.0%
TAGCAAAACACTACTCGTAC+TGG + chr4_4:60190148-60190167 Msa0691880:intron 40.0%
! CAAAGGGGTGTATAGAAAAG+GGG - chr4_4:60190219-60190238 None:intergenic 40.0%
TCATGCAATAAGGGGTAGTT+TGG + chr4_4:60190275-60190294 Msa0691880:intron 40.0%
! GATTTGTTCAGAAGAGGAAG+AGG - chr4_4:60190330-60190349 None:intergenic 40.0%
!! AAGGTCGATTTGTTCAGAAG+AGG - chr4_4:60190336-60190355 None:intergenic 40.0%
!! AGTTGATGGAGGTGATAGAA+CGG - chr4_4:60190403-60190422 None:intergenic 40.0%
AGGAGTTGTGATTCGATAGT+GGG - chr4_4:60190426-60190445 None:intergenic 40.0%
AAGGAGTTGTGATTCGATAG+TGG - chr4_4:60190427-60190446 None:intergenic 40.0%
GAGGAAAACGCAAAAAGAGT+GGG - chr4_4:60190479-60190498 None:intergenic 40.0%
TCAGTAATTGCAAATGGGGA+TGG + chr4_4:60190830-60190849 Msa0691880:intron 40.0%
CAGTAATTGCAAATGGGGAT+GGG + chr4_4:60190831-60190850 Msa0691880:intron 40.0%
!! TAATGGATAGAGGCATTGAG+TGG + chr4_4:60191438-60191457 Msa0691880:intron 40.0%
CTTTCCAATCAGCTACTCAT+GGG + chr4_4:60192104-60192123 Msa0691880:CDS 40.0%
TTTCCAATCAGCTACTCATG+GGG + chr4_4:60192105-60192124 Msa0691880:CDS 40.0%
TTAGCGGGAAGAAGAAGACA+TGG - chr4_4:60189029-60189048 None:intergenic 45.0%
! AGTGAGGAAGATGATGGTTG+TGG - chr4_4:60189054-60189073 None:intergenic 45.0%
TCAACGACGATGGAATCTGA+GGG - chr4_4:60189082-60189101 None:intergenic 45.0%
ATCAACGACGATGGAATCTG+AGG - chr4_4:60189083-60189102 None:intergenic 45.0%
!! GAACATAAGGCATCGTTGCA+AGG + chr4_4:60189119-60189138 Msa0691880:CDS 45.0%
! TGAACTTGACTTGACACCGT+TGG - chr4_4:60189548-60189567 None:intergenic 45.0%
ACATCAGCAGTGGTACAAAG+GGG - chr4_4:60190234-60190253 None:intergenic 45.0%
CACATCAGCAGTGGTACAAA+GGG - chr4_4:60190235-60190254 None:intergenic 45.0%
AGGAAGAGGAGAAGATTGTG+GGG - chr4_4:60190316-60190335 None:intergenic 45.0%
GAGGAAGAGGAGAAGATTGT+GGG - chr4_4:60190317-60190336 None:intergenic 45.0%
AGAGGAAGAGGAGAAGATTG+TGG - chr4_4:60190318-60190337 None:intergenic 45.0%
TCCTCTTCCTCTTCTCTCTT+TGG + chr4_4:60190357-60190376 Msa0691880:intron 45.0%
!! GATTCGATAGTGGGAGTTGA+TGG - chr4_4:60190417-60190436 None:intergenic 45.0%
GGAGAGAAAGGTAGTGATGA+AGG - chr4_4:60190446-60190465 None:intergenic 45.0%
AAAAGAGTGGGAGGAAATGG+CGG - chr4_4:60190467-60190486 None:intergenic 45.0%
GCAAAAAGAGTGGGAGGAAA+TGG - chr4_4:60190470-60190489 None:intergenic 45.0%
GAAAACGCAAAAAGAGTGGG+AGG - chr4_4:60190476-60190495 None:intergenic 45.0%
GGAGGAAAACGCAAAAAGAG+TGG - chr4_4:60190480-60190499 None:intergenic 45.0%
! CTTTTTGCGTTTTCCTCCGA+CGG + chr4_4:60190482-60190501 Msa0691880:intron 45.0%
TTCAACGGAGAGAGAGATAG+CGG - chr4_4:60190508-60190527 None:intergenic 45.0%
CTCTTTGGTTGTTCCTCTGA+AGG + chr4_4:60190990-60191009 Msa0691880:intron 45.0%
CTTAATCACTACGCCTTCAG+AGG - chr4_4:60191006-60191025 None:intergenic 45.0%
TAAGGCTTGTGCTCTGTCTT+GGG - chr4_4:60191352-60191371 None:intergenic 45.0%
! CATTGAGTGGTGGAATTGGT+TGG + chr4_4:60191451-60191470 Msa0691880:intron 45.0%
GCAGCCATCTGCTCATAAAA+TGG - chr4_4:60191574-60191593 None:intergenic 45.0%
TACACTGACATAGCCTCCTT+AGG + chr4_4:60191646-60191665 Msa0691880:CDS 45.0%
ACAGAATTACATCGGGACAC+GGG - chr4_4:60191951-60191970 None:intergenic 45.0%
GACAGAATTACATCGGGACA+CGG - chr4_4:60191952-60191971 None:intergenic 45.0%
CATCAGCACGCAAGACTATA+TGG - chr4_4:60191994-60192013 None:intergenic 45.0%
TGAGTAGCTGATTGGAAAGC+TGG - chr4_4:60192103-60192122 None:intergenic 45.0%
GCTTTCCAATCAGCTACTCA+TGG + chr4_4:60192103-60192122 Msa0691880:CDS 45.0%
GCAACGATGATCAACGACGA+TGG - chr4_4:60189092-60189111 None:intergenic 50.0%
CGTCGTTGATCATCGTTGCA+AGG + chr4_4:60189093-60189112 Msa0691880:CDS 50.0%
GACTTGACACCGTTGGCAAT+CGG - chr4_4:60189541-60189560 None:intergenic 50.0%
! TCGTACTGGCCAAAACCAAG+TGG + chr4_4:60190162-60190181 Msa0691880:intron 50.0%
CCACATCAGCAGTGGTACAA+AGG - chr4_4:60190236-60190255 None:intergenic 50.0%
CCTTTGTACCACTGCTGATG+TGG + chr4_4:60190233-60190252 Msa0691880:intron 50.0%
CTGATGTGGCAGGGGTAATT+TGG + chr4_4:60190247-60190266 Msa0691880:intron 50.0%
GAGAGAAGAGGAAGAGGAGA+AGG - chr4_4:60190355-60190374 None:intergenic 50.0%
GCCAAAGAGAGAAGAGGAAG+AGG - chr4_4:60190361-60190380 None:intergenic 50.0%
! AGGTGATAGAACGGCATCGA+CGG - chr4_4:60190394-60190413 None:intergenic 50.0%
!! TCGATAGTGGGAGTTGATGG+AGG - chr4_4:60190414-60190433 None:intergenic 50.0%
GCTATCTCTCTCTCCGTTGA+AGG + chr4_4:60190507-60190526 Msa0691880:intron 50.0%
GTAAGGCTTGTGCTCTGTCT+TGG - chr4_4:60191353-60191372 None:intergenic 50.0%
!! TGGATAGAGGCATTGAGTGG+TGG + chr4_4:60191441-60191460 Msa0691880:intron 50.0%
!! GAGGCATTGAGTGGTGGAAT+TGG + chr4_4:60191447-60191466 Msa0691880:intron 50.0%
CAGAATTACATCGGGACACG+GGG - chr4_4:60191950-60191969 None:intergenic 50.0%
TCCTGTCTGTGTAGGAACTC+TGG + chr4_4:60192045-60192064 Msa0691880:intron 50.0%
ACTCTGGCAAAGTACACTGC+TGG + chr4_4:60192061-60192080 Msa0691880:CDS 50.0%
TCTCCCCATGAGTAGCTGAT+TGG - chr4_4:60192111-60192130 None:intergenic 50.0%
ACCTCACAAGAGAGACTGCA+TGG + chr4_4:60192155-60192174 Msa0691880:CDS 50.0%
CCTCACAAGAGAGACTGCAT+GGG + chr4_4:60192156-60192175 Msa0691880:CDS 50.0%
GCGGGAAGAAGAAGACATGG+AGG - chr4_4:60189026-60189045 None:intergenic 55.0%
TGAGCTTGGCCGATTGCCAA+CGG + chr4_4:60189529-60189548 Msa0691880:intron 55.0%
TGTACCACTGCTGATGTGGC+AGG + chr4_4:60190237-60190256 Msa0691880:intron 55.0%
GTACCACTGCTGATGTGGCA+GGG + chr4_4:60190238-60190257 Msa0691880:intron 55.0%
TACCACTGCTGATGTGGCAG+GGG + chr4_4:60190239-60190258 Msa0691880:intron 55.0%
GGTGAGGCCAAAGAGAGAAG+AGG - chr4_4:60190367-60190386 None:intergenic 55.0%
! GGAGGAAATGGCGGAGAGAA+AGG - chr4_4:60190458-60190477 None:intergenic 55.0%
GCCAGAGTTCCTACACAGAC+AGG - chr4_4:60192049-60192068 None:intergenic 55.0%
CCCATGCAGTCTCTCTTGTG+AGG - chr4_4:60192159-60192178 None:intergenic 55.0%
GAGAGACTGCATGGGAAGCA+TGG + chr4_4:60192164-60192183 Msa0691880:CDS 55.0%
TACCCCTGCCACATCAGCAG+TGG - chr4_4:60190244-60190263 None:intergenic 60.0%
!! TAGAACGGCATCGACGGCGA+CGG - chr4_4:60190388-60190407 None:intergenic 60.0%
! GAGAGAGAGATAGCGGCCGT+CGG - chr4_4:60190501-60190520 None:intergenic 60.0%
! AGAGAGATAGCGGCCGTCGG+AGG - chr4_4:60190498-60190517 None:intergenic 65.0%
!! CGGCATCGACGGCGACGGTG+AGG - chr4_4:60190383-60190402 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 60189023 60192216 60189023 ID=Msa0691880;Name=Msa0691880
chr4_4 mRNA 60189023 60192216 60189023 ID=Msa0691880-mRNA-1;Parent=Msa0691880;Name=Msa0691880-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|0|0|0.66|0.5|0.33|3|0|141
chr4_4 exon 60189023 60189158 60189023 ID=Msa0691880-mRNA-1:exon:16465;Parent=Msa0691880-mRNA-1
chr4_4 exon 60191539 60191667 60191539 ID=Msa0691880-mRNA-1:exon:16466;Parent=Msa0691880-mRNA-1
chr4_4 exon 60192059 60192216 60192059 ID=Msa0691880-mRNA-1:exon:16467;Parent=Msa0691880-mRNA-1
chr4_4 CDS 60189023 60189158 60189023 ID=Msa0691880-mRNA-1:cds;Parent=Msa0691880-mRNA-1
chr4_4 CDS 60191539 60191667 60191539 ID=Msa0691880-mRNA-1:cds;Parent=Msa0691880-mRNA-1
chr4_4 CDS 60192059 60192216 60192059 ID=Msa0691880-mRNA-1:cds;Parent=Msa0691880-mRNA-1
Gene Sequence

>Msa0691880

CCTCCATGTCTTCTTCTTCCCGCTAAAACCACAACCATCATCTTCCTCACTAAAAACCCTCAGATTCCATCGTCGTTGATCATCGTTGCAAGGATTGAACATAAGGCATCGTTGCAAGGATTGAACATAAGGAAAGATAAAGAACATGGACTGAGACTGCCTGAAGATCCATTTTATGAGCAGATGGCTGCTGAGTTTTTCAAGTACCAAATCTCAGTAAATGTGTATGCATTCAGTGATAAGTACACTGACATAGCCTCCTTAGGAACTCTGGCAAAGTACACTGCTGGTCAAGTGTATTACTTTCCAGCTTTCCAATCAGCTACTCATGGGGAGAAATTGAGTCATGAATTAAGGAGATACCTCACAAGAGAGACTGCATGGGAAGCATGGATCATATGCATTGCAGAGATACTTAGTAAC
Protein sequence

>Msa0691880

PPCLLLPAKTTTIIFLTKNPQIPSSLIIVARIEHKASLQGLNIRKDKEHGLRLPEDPFYEQMAAEFFKYQISVNVYAFSDKYTDIASLGTLAKYTAGQVYYFPAFQSATHGEKLSHELRRYLTRETAWEAWIICIAEILSN