AlfalfaGEDB | Msa0456200

M. sativa cultivar zhongmu-4 / Msa0456200

KEGG: K14007
GO: GO:0000003 GO:0006275 GO:0006996 GO:0007029 GO:0007030 GO:0007276 GO:0008150 GO:0008156 GO:0008361 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010256 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032504 GO:0032535 GO:0032875 GO:0032876 GO:0044703 GO:0045786 GO:0045934 GO:0048232 GO:0048519 GO:0048523 GO:0048609 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051704 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0071840 GO:0080090 GO:0080119 GO:0090066 GO:0090329 GO:2000104 GO:2000112 GO:2000113
PFAM: PF04811.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456200	AES75326.1	88.750	80	9	0	1	80	1	80	7.81e-44	146

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456200	sp\|Q9SFU0\|SC24A_ARATH	76.812	69	16	0	1	69	620	688	9.06e-32	119

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456200	G7KIB7	88.750	80	9	0	1	80	1	80	3.73e-44	146

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0078590	Msa0456200	0.816674	4.897267e-52	-8.615850e-47
Msa0122590	Msa0456200	0.802222	6.215124e-49	-8.615850e-47
Msa0145250	Msa0456200	0.808917	2.446616e-50	-8.615850e-47
Msa0145750	Msa0456200	0.800999	1.107390e-48	-8.615850e-47
Msa0153200	Msa0456200	0.802447	5.585439e-49	-8.615850e-47
Msa0153910	Msa0456200	0.850595	1.534164e-60	-8.615850e-47
Msa0157810	Msa0456200	0.809788	1.591436e-50	-8.615850e-47
Msa0199240	Msa0456200	0.802422	5.652212e-49	-8.615850e-47
Msa0201250	Msa0456200	0.804903	1.727284e-49	-8.615850e-47
Msa0203500	Msa0456200	0.806993	6.280049e-50	-8.615850e-47
Msa0233300	Msa0456200	0.829860	4.064982e-55	-8.615850e-47
Msa0399300	Msa0456200	0.815066	1.118292e-51	-8.615850e-47
Msa0402290	Msa0456200	0.804800	1.815652e-49	-8.615850e-47
Msa0406120	Msa0456200	0.821030	5.015374e-53	-8.615850e-47
Msa0414230	Msa0456200	0.801840	7.446777e-49	-8.615850e-47
Msa0417100	Msa0456200	0.803355	3.626843e-49	-8.615850e-47
Msa0431500	Msa0456200	0.884777	1.435197e-71	-8.615850e-47
Msa0431690	Msa0456200	0.819406	1.181221e-52	-8.615850e-47
Msa0432290	Msa0456200	0.821932	3.104050e-53	-8.615850e-47
Msa0437990	Msa0456200	0.830167	3.420626e-55	-8.615850e-47
Msa0452620	Msa0456200	0.842441	2.581322e-58	-8.615850e-47
Msa0456200	Msa0458090	0.800266	1.562481e-48	-8.615850e-47
Msa0456200	Msa0482620	0.820771	5.750399e-53	-8.615850e-47
Msa0456200	Msa0483650	0.811063	8.442876e-51	-8.615850e-47
Msa0456200	Msa0484780	0.814257	1.689260e-51	-8.615850e-47
Msa0456200	Msa0502270	0.801021	1.095738e-48	-8.615850e-47
Msa0456200	Msa0512900	0.820398	7.005535e-53	-8.615850e-47
Msa0456200	Msa0527680	0.800633	1.315315e-48	-8.615850e-47
Msa0456200	Msa0532350	0.801870	7.341958e-49	-8.615850e-47
Msa0456200	Msa0533290	0.800689	1.281032e-48	-8.615850e-47
Msa0456200	Msa0533430	0.831538	1.577574e-55	-8.615850e-47
Msa0456200	Msa0546710	0.808128	3.605408e-50	-8.615850e-47
Msa0456200	Msa0548060	0.805100	1.570905e-49	-8.615850e-47
Msa0456200	Msa0558640	0.809073	2.265840e-50	-8.615850e-47
Msa0456200	Msa0627410	0.990113	4.063297e-181	-8.615850e-47
Msa0456200	Msa0639460	0.806911	6.536154e-50	-8.615850e-47
Msa0456200	Msa0657970	0.838386	2.966709e-57	-8.615850e-47
Msa0456200	Msa0661870	0.838861	2.235745e-57	-8.615850e-47
Msa0456200	Msa0673450	0.802804	4.714935e-49	-8.615850e-47
Msa0456200	Msa0678930	0.819010	1.453490e-52	-8.615850e-47
Msa0456200	Msa0682360	0.825223	5.271290e-54	-8.615850e-47
Msa0456200	Msa0691880	0.854582	1.118501e-61	-8.615850e-47
Msa0456200	Msa0726140	0.813469	2.519721e-51	-8.615850e-47
Msa0456200	Msa0741570	0.834824	2.397300e-56	-8.615850e-47
Msa0456200	Msa0745060	0.818224	2.191911e-52	-8.615850e-47
Msa0456200	Msa0762360	0.808508	2.991911e-50	-8.615850e-47
Msa0456200	Msa0766630	0.811071	8.411647e-51	-8.615850e-47
Msa0456200	Msa0777930	0.801549	8.545114e-49	-8.615850e-47
Msa0456200	Msa0789250	0.804738	1.870593e-49	-8.615850e-47
Msa0456200	Msa0793640	0.811135	8.146350e-51	-8.615850e-47
Msa0456200	Msa0815320	0.811095	8.311314e-51	-8.615850e-47
Msa0456200	Msa0822650	0.815363	9.606316e-52	-8.615850e-47
Msa0456200	Msa0825860	0.813227	2.847162e-51	-8.615850e-47
Msa0456200	Msa0831970	0.801153	1.029994e-48	-8.615850e-47
Msa0456200	Msa0840740	0.804151	2.478322e-49	-8.615850e-47
Msa0456200	Msa0853550	0.808664	2.771970e-50	-8.615850e-47
Msa0456200	Msa0885280	0.866919	2.004584e-65	-8.615850e-47
Msa0456200	Msa0885290	0.858811	6.374823e-63	-8.615850e-47
Msa0456200	Msa0886750	0.803027	4.239735e-49	-8.615850e-47
Msa0456200	Msa0887990	0.871857	4.964715e-67	-8.615850e-47
Msa0456200	Msa0897880	0.817502	3.189210e-52	-8.615850e-47
Msa0456200	Msa0907070	0.811216	7.822889e-51	-8.615850e-47
Msa0456200	Msa0909120	0.836020	1.195159e-56	-8.615850e-47
Msa0456200	Msa0909140	0.811652	6.289888e-51	-8.615850e-47
Msa0456200	Msa0912500	0.807148	5.822583e-50	-8.615850e-47
Msa0456200	Msa0922720	0.822360	2.469785e-53	-8.615850e-47
Msa0456200	Msa0935910	0.802726	4.893724e-49	-8.615850e-47
Msa0456200	Msa0954910	0.816235	6.138502e-52	-8.615850e-47
Msa0456200	Msa0965750	0.852273	5.142293e-61	-8.615850e-47
Msa0456200	Msa1006250	0.816687	4.863480e-52	-8.615850e-47
Msa0456200	Msa1024240	0.826253	3.002880e-54	-8.615850e-47
Msa0456200	Msa1032850	0.800010	1.760838e-48	-8.615850e-47
Msa0456200	Msa1077870	0.818734	1.679780e-52	-8.615850e-47
Msa0456200	Msa1079180	0.816575	5.153033e-52	-8.615850e-47
Msa0456200	Msa1082500	0.818064	2.382177e-52	-8.615850e-47
Msa0456200	Msa1084240	0.815551	8.726369e-52	-8.615850e-47
Msa0456200	Msa1092390	0.803479	3.417708e-49	-8.615850e-47
Msa0456200	Msa1100320	0.804859	1.764332e-49	-8.615850e-47
Msa0456200	Msa1100980	0.810185	1.306959e-50	-8.615850e-47
Msa0456200	Msa1135740	0.801586	8.396155e-49	-8.615850e-47
Msa0456200	Msa1136090	0.844702	6.417122e-59	-8.615850e-47
Msa0456200	Msa1148020	0.822418	2.394612e-53	-8.615850e-47
Msa0456200	Msa1160670	0.843109	1.715344e-58	-8.615850e-47
Msa0456200	Msa1173920	0.815939	7.148381e-52	-8.615850e-47
Msa0456200	Msa1178720	0.910819	1.222561e-82	-8.615850e-47
Msa0456200	Msa1182730	0.804534	2.062994e-49	-8.615850e-47
Msa0456200	Msa1187940	0.842601	2.341107e-58	-8.615850e-47
Msa0456200	Msa1187950	0.813856	2.070836e-51	-8.615850e-47
Msa0456200	Msa1249490	0.800623	1.321065e-48	-8.615850e-47
Msa0456200	Msa1260100	0.805066	1.597024e-49	-8.615850e-47
Msa0456200	Msa1269670	0.802332	5.900402e-49	-8.615850e-47
Msa0456200	Msa1270270	0.800297	1.539655e-48	-8.615850e-47
Msa0456200	Msa1283860	0.816220	6.188027e-52	-8.615850e-47
Msa0456200	Msa1288000	0.825433	4.700591e-54	-8.615850e-47
Msa0456200	Msa1297210	0.854023	1.622275e-61	-8.615850e-47
Msa0456200	Msa1307980	0.807872	4.088933e-50	-8.615850e-47
Msa0456200	Msa1314280	0.801079	1.066382e-48	-8.615850e-47
Msa0456200	Msa1324780	0.838008	3.711661e-57	-8.615850e-47
Msa0456200	Msa1347360	0.804530	2.066637e-49	-8.615850e-47
Msa0456200	Msa1397610	0.848519	5.820430e-60	-8.615850e-47
Msa0456200	Msa1397860	0.807202	5.672108e-50	-8.615850e-47
Msa0456200	Msa1411020	0.823177	1.593923e-53	-8.615850e-47
Msa0456200	Msa1420140	0.802769	4.792956e-49	-8.615850e-47
Msa0456200	Msa1420490	0.808690	2.735999e-50	-8.615850e-47
Msa0456200	Msa1423390	0.810465	1.137276e-50	-8.615850e-47
Msa0456200	Msa1423990	0.805032	1.623618e-49	-8.615850e-47
Msa0456200	Msa1424290	0.824183	9.264839e-54	-8.615850e-47
Msa0456200	Msa1434350	0.824263	8.875901e-54	-8.615850e-47
Msa0456200	Msa1438250	0.845639	3.581842e-59	-8.615850e-47
Msa0456200	Msa1439320	0.822059	2.900506e-53	-8.615850e-47
Msa0456200	Msa1440370	0.811143	8.111745e-51	-8.615850e-47
Msa0456200	Msa1440810	0.810876	9.268218e-51	-8.615850e-47
Msa0456200	Msa1453720	0.809910	1.498136e-50	-8.615850e-47
Msa0256440	Msa0456200	0.805243	1.466367e-49	-8.615850e-47
Msa0311630	Msa0456200	0.800009	1.762272e-48	-8.615850e-47
Msa0324150	Msa0456200	0.808379	3.188615e-50	-8.615850e-47
Msa0326200	Msa0456200	0.978475	6.661342e-146	-8.615850e-47
Msa0353190	Msa0456200	0.967322	4.061932e-127	-8.615850e-47
Msa0353690	Msa0456200	0.955744	1.494335e-113	-8.615850e-47
Msa0360900	Msa0456200	0.883388	4.681349e-71	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456200	MtrunA17_Chr7g0271481	91.304	69	6	0	1	69	602	670	2.26e-39	137
Msa0456200	MtrunA17_Chr1g0210851	39.130	69	42	0	1	69	699	767	4.14e-12	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456200	AT3G07100.1	76.812	69	16	0	1	69	620	688	9.21e-33	119

Find 15 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAAATTGAGTCATGAATTA+AGG	0.308061	3_3:-49664159	Msa0456200:CDS
CATACACATTTACTGAGATT+TGG	0.310882	3_3:+49664678	None:intergenic
TCCTGTCTGTGTAGGAACTC+TGG	0.429146	3_3:-49664242	Msa0456200:intron
TCTCCCCATGAGTAGCTGAT+TGG	0.442822	3_3:+49664179	None:intergenic
TGAGTAGCTGATTGGAAAGC+TGG	0.469035	3_3:+49664187	None:intergenic
GAGAGACTGCATGGGAAGCA+TGG	0.524555	3_3:-49664123	Msa0456200:CDS
GCTTTCCAATCAGCTACTCA+TGG	0.528786	3_3:-49664184	Msa0456200:CDS
TACACTGACATAGCCTCCTT+AGG	0.536987	3_3:-49664641	Msa0456200:intron
CTTTCCAATCAGCTACTCAT+GGG	0.542155	3_3:-49664183	Msa0456200:CDS
GGAAAATAAAACACCTAAGG+AGG	0.554451	3_3:+49664628	None:intergenic
ACCTCACAAGAGAGACTGCA+TGG	0.560035	3_3:-49664132	Msa0456200:CDS
ACTCTGGCAAAGTACACTGC+TGG	0.591069	3_3:-49664226	Msa0456200:CDS
CCCATGCAGTCTCTCTTGTG+AGG	0.598716	3_3:+49664131	None:intergenic
TTTCCAATCAGCTACTCATG+GGG	0.686768	3_3:-49664182	Msa0456200:CDS
CCTCACAAGAGAGACTGCAT+GGG	0.693489	3_3:-49664131	Msa0456200:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACGAAATACATAGAAATTGA+AGG	+	chr3_3:49664187-49664206	None:intergenic	25.0%
!	TAACAGGTTTAAAAATGGAA+TGG	+	chr3_3:49664214-49664233	None:intergenic	25.0%
!	AAGAGTAACAGGTTTAAAAA+TGG	+	chr3_3:49664219-49664238	None:intergenic	25.0%
!	ATCAATTAATCAAGAGTAAC+AGG	+	chr3_3:49664230-49664249	None:intergenic	25.0%
!	AGAAATTGAGTCATGAATTA+AGG	-	chr3_3:49664632-49664651	Msa0456200:intron	25.0%
	CATACACATTTACTGAGATT+TGG	+	chr3_3:49664116-49664135	None:intergenic	30.0%
	GAAGGAAAATAAAACACCTA+AGG	+	chr3_3:49664169-49664188	None:intergenic	30.0%
!	ATTTGATGATTCTTCAGTTG+TGG	-	chr3_3:49664252-49664271	Msa0456200:intron	30.0%
!	ACTTGATGACATGAGTTTAT+GGG	-	chr3_3:49664290-49664309	Msa0456200:intron	30.0%
	TAAACTGATACTCTAACTCA+TGG	-	chr3_3:49664333-49664352	Msa0456200:intron	30.0%
	AAATGCTGACAGAATTACAT+CGG	+	chr3_3:49664463-49664482	None:intergenic	30.0%
	GGAAAATAAAACACCTAAGG+AGG	+	chr3_3:49664166-49664185	None:intergenic	35.0%
!	GACTTGATGACATGAGTTTA+TGG	-	chr3_3:49664289-49664308	Msa0456200:intron	35.0%
	GGATCTATTTGACTATGAAC+TGG	-	chr3_3:49664354-49664373	Msa0456200:intron	35.0%
	AATGCTGACAGAATTACATC+GGG	+	chr3_3:49664462-49664481	None:intergenic	35.0%
	TGTAATTCTGTCAGCATTTG+CGG	-	chr3_3:49664464-49664483	Msa0456200:intron	35.0%
!!!	GTTTTTTTTCCTGTCTGTGT+AGG	-	chr3_3:49664541-49664560	Msa0456200:intron	35.0%
	CTTTCCAATCAGCTACTCAT+GGG	-	chr3_3:49664608-49664627	Msa0456200:intron	40.0%
	TTTCCAATCAGCTACTCATG+GGG	-	chr3_3:49664609-49664628	Msa0456200:intron	40.0%
	TACACTGACATAGCCTCCTT+AGG	-	chr3_3:49664150-49664169	Msa0456200:CDS	45.0%
	ACAGAATTACATCGGGACAC+GGG	+	chr3_3:49664455-49664474	None:intergenic	45.0%
	GACAGAATTACATCGGGACA+CGG	+	chr3_3:49664456-49664475	None:intergenic	45.0%
	CATCAGCACGCAAGACTATA+TGG	+	chr3_3:49664498-49664517	None:intergenic	45.0%
	TGAGTAGCTGATTGGAAAGC+TGG	+	chr3_3:49664607-49664626	None:intergenic	45.0%
	GCTTTCCAATCAGCTACTCA+TGG	-	chr3_3:49664607-49664626	Msa0456200:intron	45.0%
	CAGAATTACATCGGGACACG+GGG	+	chr3_3:49664454-49664473	None:intergenic	50.0%
	TCCTGTCTGTGTAGGAACTC+TGG	-	chr3_3:49664549-49664568	Msa0456200:intron	50.0%
	ACTCTGGCAAAGTACACTGC+TGG	-	chr3_3:49664565-49664584	Msa0456200:intron	50.0%
	TCTCCCCATGAGTAGCTGAT+TGG	+	chr3_3:49664615-49664634	None:intergenic	50.0%
	ACCTCACAAGAGAGACTGCA+TGG	-	chr3_3:49664659-49664678	Msa0456200:CDS	50.0%
	CCTCACAAGAGAGACTGCAT+GGG	-	chr3_3:49664660-49664679	Msa0456200:CDS	50.0%
	GCCAGAGTTCCTACACAGAC+AGG	+	chr3_3:49664553-49664572	None:intergenic	55.0%
	CCCATGCAGTCTCTCTTGTG+AGG	+	chr3_3:49664663-49664682	None:intergenic	55.0%
	GAGAGACTGCATGGGAAGCA+TGG	-	chr3_3:49664668-49664687	Msa0456200:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	49664090	49664723	49664090	ID=Msa0456200;Name=Msa0456200
chr3_3	mRNA	49664090	49664723	49664090	ID=Msa0456200-mRNA-1;Parent=Msa0456200;Name=Msa0456200-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|1\|1\|2\|0\|80
chr3_3	exon	49664642	49664723	49664642	ID=Msa0456200-mRNA-1:exon:9396;Parent=Msa0456200-mRNA-1
chr3_3	exon	49664090	49664250	49664090	ID=Msa0456200-mRNA-1:exon:9395;Parent=Msa0456200-mRNA-1
chr3_3	CDS	49664642	49664723	49664642	ID=Msa0456200-mRNA-1:cds;Parent=Msa0456200-mRNA-1
chr3_3	CDS	49664090	49664250	49664090	ID=Msa0456200-mRNA-1:cds;Parent=Msa0456200-mRNA-1

Gene Sequence	>Msa0456200 ATGGCTGTTGAGTTTTTCAAGTACCAAATCTCAGTAAATGTGTATGCATTCAGTGATAAGTACACTGACATAGCCTCCTTAGGAACTCTGGCAAAGTACACTGCTGGTCAAGTGTATTACTTTCCAGCTTTCCAATCAGCTACTCATGGGGAGAAATTGAGTCATGAATTAAGGAGATACCTCACAAGAGAGACTGCATGGGAAGCATGGATCATATGCATTGCAGAGATACTTAGTAACTAG
Protein sequence	>Msa0456200 MAVEFFKYQISVNVYAFSDKYTDIASLGTLAKYTAGQVYYFPAFQSATHGEKLSHELRRYLTRETAWEAWIICIAEILSN*