AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0692050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0692050 MtrunA17_Chr4g0042051 95.238 126 6 0 1 126 1 126 1.56e-87 250
Msa0692050 MtrunA17_Chr2g0319291 70.492 122 36 0 1 122 1 122 8.55e-63 187
Msa0692050 MtrunA17_Chr2g0280411 60.169 118 47 0 6 123 5 122 3.07e-50 155
Msa0692050 MtrunA17_Chr7g0216481 41.237 97 57 0 17 113 33 129 8.12e-25 91.7
Msa0692050 MtrunA17_Chr7g0245141 35.294 102 66 0 12 113 26 127 2.23e-22 85.5
Msa0692050 MtrunA17_Chr1g0155721 36.893 103 65 0 8 110 4 106 9.68e-22 83.6
Msa0692050 MtrunA17_Chr3g0142051 35.455 110 67 1 1 110 1 106 2.59e-21 82.4
Msa0692050 MtrunA17_Chr5g0404831 35.088 114 72 1 1 114 1 112 1.05e-20 80.9
Msa0692050 MtrunA17_Chr8g0334481 33.333 99 66 0 15 113 31 129 1.13e-20 81.3
Msa0692050 MtrunA17_Chr5g0415771 38.053 113 66 2 4 114 13 123 7.68e-19 76.3
Msa0692050 MtrunA17_Chr8g0393201 31.132 106 73 0 8 113 225 330 4.41e-18 78.2
Msa0692050 MtrunA17_Chr5g0415761 34.286 105 69 0 10 114 20 124 5.95e-17 71.2
Msa0692050 MtrunA17_Chr5g0415741 35.238 105 68 0 10 114 18 122 2.20e-16 70.1
Msa0692050 MtrunA17_Chr1g0201781 33.721 86 55 2 35 118 81 166 5.01e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0692050 AT1G11530.1 57.143 112 48 0 10 121 2 113 9.79e-48 149
Msa0692050 AT3G51030.1 38.679 106 65 0 8 113 4 109 2.03e-23 87.8
Msa0692050 AT5G39950.1 37.037 108 68 0 6 113 21 128 3.63e-23 87.8
Msa0692050 AT3G08710.2 36.697 109 69 0 15 123 28 136 4.91e-23 87.8
Msa0692050 AT3G08710.3 36.697 109 69 0 15 123 28 136 4.91e-23 87.8
Msa0692050 AT3G08710.1 36.697 109 69 0 15 123 28 136 4.91e-23 87.8
Msa0692050 AT3G56420.1 37.963 108 67 0 5 112 35 142 7.08e-23 87.8
Msa0692050 AT3G56420.2 37.963 108 67 0 5 112 35 142 7.08e-23 87.8
Msa0692050 AT3G56420.3 37.963 108 67 0 5 112 35 142 7.08e-23 87.8
Msa0692050 AT1G45145.1 34.286 105 69 0 9 113 4 108 2.00e-22 85.5
Msa0692050 AT2G40790.2 35.577 104 67 0 9 112 39 142 4.03e-22 85.9
Msa0692050 AT2G40790.1 35.577 104 67 0 9 112 39 142 4.03e-22 85.9
Msa0692050 AT1G59730.1 34.615 104 68 0 12 115 23 126 2.94e-21 82.8
Msa0692050 AT5G42980.1 33.962 106 70 0 9 114 4 109 9.02e-19 76.3
Msa0692050 AT1G19730.1 30.841 107 73 1 8 113 4 110 5.16e-18 74.3
Msa0692050 AT1G69880.1 28.571 105 75 0 12 116 39 143 1.08e-16 71.6
Msa0692050 AT3G17880.1 33.333 114 72 1 11 124 271 380 1.97e-15 71.2
Msa0692050 AT3G17880.2 33.333 114 72 1 11 124 264 373 1.99e-15 71.2
Msa0692050 AT2G35010.2 29.907 107 73 1 12 116 86 192 9.28e-12 59.7
Msa0692050 AT2G35010.1 29.907 107 73 1 12 116 86 192 9.28e-12 59.7

Find 42 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTGTTGTCTTATTTATA+AGG 0.224668 4_4:-60382637 Msa0692050:five_prime_UTR
ACTTACAAGGCAACCTTTAT+TGG 0.239798 4_4:+60382420 None:intergenic
TTTGAAGAAGTAGAAAATAA+TGG 0.308037 4_4:-60382524 Msa0692050:exon
GTGCAAATCCTGATGAAATT+AGG 0.320699 4_4:-60381020 Msa0692050:CDS
TCCTCCACTCATCAACAAAA+AGG 0.360970 4_4:+60381065 None:intergenic
ATCACTGAAGCTACCAATAA+AGG 0.364334 4_4:-60382433 Msa0692050:CDS
TTGAAGAAGTAGAAAATAAT+GGG 0.365131 4_4:-60382523 Msa0692050:exon
GTGATTTGGAGGAATGAGTT+TGG 0.374475 4_4:+60380974 None:intergenic
CATGAGCATAGCTGAAGAAT+TGG 0.376383 4_4:-60382713 Msa0692050:five_prime_UTR
CATGGCATTGATTTCCAACT+TGG 0.380007 4_4:+60381092 None:intergenic
CATCATCCATCAGAGTGATT+TGG 0.391841 4_4:+60380960 None:intergenic
GGTGGATGTGGACGAAGTTA+AGG 0.395797 4_4:-60382125 Msa0692050:intron
TATAACCAATCTTATCTAGC+TGG 0.426879 4_4:+60382677 None:intergenic
CTGAAGAATTGGTAACATAA+TGG 0.428650 4_4:-60382702 Msa0692050:five_prime_UTR
GGCATTGATTTCCAACTTGG+AGG 0.441850 4_4:+60381095 None:intergenic
CAGAAAGAGGACATCTTGAT+AGG 0.447661 4_4:+60382150 None:intergenic
AAAGGGTTCATAGCAATAGA+AGG 0.450955 4_4:+60382193 None:intergenic
GATGCGAATTCTTCAAAGAA+AGG 0.458372 4_4:+60382175 None:intergenic
CCTCTTTCTGAAGGTGGATG+TGG 0.473122 4_4:-60382137 Msa0692050:CDS
ATAACCAATCTTATCTAGCT+GGG 0.488311 4_4:+60382678 None:intergenic
GCACCAACAGCTTTCTCCAC+TGG 0.488674 4_4:+60381039 None:intergenic
TTGTGTATATATACAAACTC+TGG 0.503825 4_4:+60382555 None:intergenic
CCACATCCACCTTCAGAAAG+AGG 0.505638 4_4:+60382137 None:intergenic
ATCCTGATGAAATTAGGAAA+AGG 0.511549 4_4:-60381014 Msa0692050:CDS
CATCCATCAGAGTGATTTGG+AGG 0.522008 4_4:+60380963 None:intergenic
ATTCCTCCAAATCACTCTGA+TGG 0.522941 4_4:-60380966 Msa0692050:exon
TGGCCCCAGCTAGATAAGAT+TGG 0.526897 4_4:-60382682 Msa0692050:five_prime_UTR
ATGCGAATTCTTCAAAGAAA+GGG 0.531001 4_4:+60382176 None:intergenic
AAATTGACTCAAGAAAATCA+TGG 0.550454 4_4:-60382465 Msa0692050:CDS
AATTGACTCAAGAAAATCAT+GGG 0.553360 4_4:-60382464 Msa0692050:CDS
TATTGGTAGCTTCAGTGATG+TGG 0.558401 4_4:+60382437 None:intergenic
TCAAGATGTCCTCTTTCTGA+AGG 0.562276 4_4:-60382146 Msa0692050:CDS
AGTATATATATAGTTTGTGT+AGG 0.565246 4_4:-60382594 Msa0692050:five_prime_UTR
AGATGTCCTCTTTCTGAAGG+TGG 0.570239 4_4:-60382143 Msa0692050:CDS
TGATGAAATTAGGAAAAGGA+TGG 0.577508 4_4:-60381010 Msa0692050:CDS
TAGAATGTATATTATTACAC+AGG 0.591800 4_4:+60380856 None:intergenic
CTAGGAAGTAGCCTCCAAGT+TGG 0.602024 4_4:-60381106 Msa0692050:intron
AATCCAGTGGAGAAAGCTGT+TGG 0.602305 4_4:-60381042 Msa0692050:CDS
AAAGAGGACATCTTGATAGG+TGG 0.610226 4_4:+60382153 None:intergenic
TAACCAATCTTATCTAGCTG+GGG 0.641666 4_4:+60382679 None:intergenic
AAGAAGTAGAAAATAATGGG+TGG 0.661165 4_4:-60382520 Msa0692050:exon
GATGAGTGGAGGAAATCCAG+TGG 0.727295 4_4:-60381055 Msa0692050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTATATAGTTATATTAAT+AGG - chr4_4:60381275-60381294 Msa0692050:intron 10.0%
!!! ATGATAAAAGATATTATTTT+AGG + chr4_4:60382008-60382027 None:intergenic 10.0%
!! TAAATAAAAAAATACATGTA+GGG - chr4_4:60382248-60382267 Msa0692050:intron 10.0%
!! ATTACATAACATATTATTAT+TGG + chr4_4:60382349-60382368 None:intergenic 10.0%
!! ATCTATATAGTTATATTAAT+AGG - chr4_4:60381275-60381294 Msa0692050:intron 10.0%
!!! ATGATAAAAGATATTATTTT+AGG + chr4_4:60382008-60382027 None:intergenic 10.0%
!! TAAATAAAAAAATACATGTA+GGG - chr4_4:60382248-60382267 Msa0692050:intron 10.0%
!! ATTACATAACATATTATTAT+TGG + chr4_4:60382349-60382368 None:intergenic 10.0%
!! CATGTAAAATTATAACAATT+GGG - chr4_4:60381222-60381241 Msa0692050:intron 15.0%
!! TTTAAAAACACAAATGAAAA+AGG + chr4_4:60381506-60381525 None:intergenic 15.0%
!! AATAAACAATTTGAGAATAT+TGG + chr4_4:60381844-60381863 None:intergenic 15.0%
!! ACAAATTATATTGAAACAAA+AGG + chr4_4:60382091-60382110 None:intergenic 15.0%
!!! AATATAATTTGTGAATTTTC+TGG - chr4_4:60382099-60382118 Msa0692050:intron 15.0%
!! CTAAATAAAAAAATACATGT+AGG - chr4_4:60382247-60382266 Msa0692050:intron 15.0%
!! CATGTAAAATTATAACAATT+GGG - chr4_4:60381222-60381241 Msa0692050:intron 15.0%
!! TTTAAAAACACAAATGAAAA+AGG + chr4_4:60381506-60381525 None:intergenic 15.0%
!! AATAAACAATTTGAGAATAT+TGG + chr4_4:60381844-60381863 None:intergenic 15.0%
!! ACAAATTATATTGAAACAAA+AGG + chr4_4:60382091-60382110 None:intergenic 15.0%
!!! AATATAATTTGTGAATTTTC+TGG - chr4_4:60382099-60382118 Msa0692050:intron 15.0%
!! CTAAATAAAAAAATACATGT+AGG - chr4_4:60382247-60382266 Msa0692050:intron 15.0%
!!! CAATTGTTGTCTTATTTATA+AGG - chr4_4:60380935-60380954 Msa0692050:three_prime_UTR 20.0%
!!! AGTATATATATAGTTTGTGT+AGG - chr4_4:60380978-60380997 Msa0692050:CDS 20.0%
!!! TATTTTCTACTTCTTCAAAA+GGG + chr4_4:60381047-60381066 None:intergenic 20.0%
!!! TTATTTTCTACTTCTTCAAA+AGG + chr4_4:60381048-60381067 None:intergenic 20.0%
!! TTTGAAGAAGTAGAAAATAA+TGG - chr4_4:60381048-60381067 Msa0692050:CDS 20.0%
!! TTGAAGAAGTAGAAAATAAT+GGG - chr4_4:60381049-60381068 Msa0692050:CDS 20.0%
!!! TAAAATTGATGATACTTACA+AGG + chr4_4:60381168-60381187 None:intergenic 20.0%
!!! TGTAAGTATCATCAATTTTA+TGG - chr4_4:60381168-60381187 Msa0692050:intron 20.0%
!! GCATGTAAAATTATAACAAT+TGG - chr4_4:60381221-60381240 Msa0692050:intron 20.0%
!! TTTGTTCATAAAATATTTGC+AGG - chr4_4:60381324-60381343 Msa0692050:intron 20.0%
!!! GAAGTTAAGGTAATTTTTTA+TGG - chr4_4:60381460-60381479 Msa0692050:intron 20.0%
!! ATGCCATTATTATAAGTTAA+AGG + chr4_4:60381954-60381973 None:intergenic 20.0%
!! AATTATATTGAAACAAAAGG+TGG + chr4_4:60382088-60382107 None:intergenic 20.0%
!!! ATTAGTTTAGAAAATAAGCA+TGG + chr4_4:60382227-60382246 None:intergenic 20.0%
!!! CAATTGTTGTCTTATTTATA+AGG - chr4_4:60380935-60380954 Msa0692050:three_prime_UTR 20.0%
!!! AGTATATATATAGTTTGTGT+AGG - chr4_4:60380978-60380997 Msa0692050:CDS 20.0%
!!! TATTTTCTACTTCTTCAAAA+GGG + chr4_4:60381047-60381066 None:intergenic 20.0%
!!! TTATTTTCTACTTCTTCAAA+AGG + chr4_4:60381048-60381067 None:intergenic 20.0%
!! TTTGAAGAAGTAGAAAATAA+TGG - chr4_4:60381048-60381067 Msa0692050:CDS 20.0%
!! TTGAAGAAGTAGAAAATAAT+GGG - chr4_4:60381049-60381068 Msa0692050:CDS 20.0%
!!! TAAAATTGATGATACTTACA+AGG + chr4_4:60381168-60381187 None:intergenic 20.0%
!!! TGTAAGTATCATCAATTTTA+TGG - chr4_4:60381168-60381187 Msa0692050:intron 20.0%
!! GCATGTAAAATTATAACAAT+TGG - chr4_4:60381221-60381240 Msa0692050:intron 20.0%
!! TTTGTTCATAAAATATTTGC+AGG - chr4_4:60381324-60381343 Msa0692050:intron 20.0%
!!! GAAGTTAAGGTAATTTTTTA+TGG - chr4_4:60381460-60381479 Msa0692050:intron 20.0%
!! ATGCCATTATTATAAGTTAA+AGG + chr4_4:60381954-60381973 None:intergenic 20.0%
!! AATTATATTGAAACAAAAGG+TGG + chr4_4:60382088-60382107 None:intergenic 20.0%
!!! ATTAGTTTAGAAAATAAGCA+TGG + chr4_4:60382227-60382246 None:intergenic 20.0%
! TTGTGTATATATACAAACTC+TGG + chr4_4:60381020-60381039 None:intergenic 25.0%
!! AAATTGACTCAAGAAAATCA+TGG - chr4_4:60381107-60381126 Msa0692050:intron 25.0%
!! AATTGACTCAAGAAAATCAT+GGG - chr4_4:60381108-60381127 Msa0692050:intron 25.0%
! TTATAACAATTGGGTGATTA+TGG - chr4_4:60381231-60381250 Msa0692050:intron 25.0%
! CATAAAATATTTGCAGGTTA+TGG - chr4_4:60381330-60381349 Msa0692050:intron 25.0%
!!! AGGTAATTTTTTATGGTCTA+TGG - chr4_4:60381467-60381486 Msa0692050:intron 25.0%
!!! TAATTTTTTATGGTCTATGG+AGG - chr4_4:60381470-60381489 Msa0692050:intron 25.0%
!!! AATTTTTTATGGTCTATGGA+GGG - chr4_4:60381471-60381490 Msa0692050:intron 25.0%
!! CGGATAATGGTATATATATA+TGG + chr4_4:60381541-60381560 None:intergenic 25.0%
!! ACGCTTTCAATTTAAAATGT+CGG + chr4_4:60381571-60381590 None:intergenic 25.0%
! AAACTAACATGTAGTTTCTT+TGG + chr4_4:60381762-60381781 None:intergenic 25.0%
!! GAGCCTTTAACTTATAATAA+TGG - chr4_4:60381948-60381967 Msa0692050:intron 25.0%
!! CTTTTATCATCATCCTTTTT+AGG - chr4_4:60382018-60382037 Msa0692050:intron 25.0%
! TCTTCACTCAACATAAAAAT+TGG + chr4_4:60382134-60382153 None:intergenic 25.0%
!! ATGGAAGAAACTGTTTATTT+TGG - chr4_4:60382161-60382180 Msa0692050:CDS 25.0%
!! TTAACTAACTACCTTTTGTT+TGG - chr4_4:60382416-60382435 Msa0692050:intron 25.0%
! TTGTGTATATATACAAACTC+TGG + chr4_4:60381020-60381039 None:intergenic 25.0%
!! AAATTGACTCAAGAAAATCA+TGG - chr4_4:60381107-60381126 Msa0692050:intron 25.0%
!! AATTGACTCAAGAAAATCAT+GGG - chr4_4:60381108-60381127 Msa0692050:intron 25.0%
! TTATAACAATTGGGTGATTA+TGG - chr4_4:60381231-60381250 Msa0692050:intron 25.0%
! CATAAAATATTTGCAGGTTA+TGG - chr4_4:60381330-60381349 Msa0692050:intron 25.0%
!!! AGGTAATTTTTTATGGTCTA+TGG - chr4_4:60381467-60381486 Msa0692050:intron 25.0%
!!! TAATTTTTTATGGTCTATGG+AGG - chr4_4:60381470-60381489 Msa0692050:intron 25.0%
!!! AATTTTTTATGGTCTATGGA+GGG - chr4_4:60381471-60381490 Msa0692050:intron 25.0%
!! CGGATAATGGTATATATATA+TGG + chr4_4:60381541-60381560 None:intergenic 25.0%
!! ACGCTTTCAATTTAAAATGT+CGG + chr4_4:60381571-60381590 None:intergenic 25.0%
! AAACTAACATGTAGTTTCTT+TGG + chr4_4:60381762-60381781 None:intergenic 25.0%
!! GAGCCTTTAACTTATAATAA+TGG - chr4_4:60381948-60381967 Msa0692050:intron 25.0%
!! CTTTTATCATCATCCTTTTT+AGG - chr4_4:60382018-60382037 Msa0692050:intron 25.0%
! TCTTCACTCAACATAAAAAT+TGG + chr4_4:60382134-60382153 None:intergenic 25.0%
!! ATGGAAGAAACTGTTTATTT+TGG - chr4_4:60382161-60382180 Msa0692050:CDS 25.0%
!! TTAACTAACTACCTTTTGTT+TGG - chr4_4:60382416-60382435 Msa0692050:intron 25.0%
! CTGAAGAATTGGTAACATAA+TGG - chr4_4:60380870-60380889 Msa0692050:three_prime_UTR 30.0%
ATAACCAATCTTATCTAGCT+GGG + chr4_4:60380897-60380916 None:intergenic 30.0%
TATAACCAATCTTATCTAGC+TGG + chr4_4:60380898-60380917 None:intergenic 30.0%
! AAGAAGTAGAAAATAATGGG+TGG - chr4_4:60381052-60381071 Msa0692050:CDS 30.0%
!! TGGTTCATTTTTCTGCTTAT+TGG - chr4_4:60381350-60381369 Msa0692050:intron 30.0%
ATGCGAATTCTTCAAAGAAA+GGG + chr4_4:60381399-60381418 None:intergenic 30.0%
!!! ATTTTTTATGGTCTATGGAG+GGG - chr4_4:60381472-60381491 Msa0692050:intron 30.0%
! AAACTGAAAGTTTAGTTCGT+TGG + chr4_4:60381642-60381661 None:intergenic 30.0%
! CAGTTCAAAAAAGTACAGTT+CGG - chr4_4:60381702-60381721 Msa0692050:intron 30.0%
TATGATATCTCCTTCTATGT+TGG - chr4_4:60381781-60381800 Msa0692050:intron 30.0%
!! TTATCATCATCCTTTTTAGG+TGG - chr4_4:60382021-60382040 Msa0692050:intron 30.0%
ATCCTGATGAAATTAGGAAA+AGG - chr4_4:60382558-60382577 Msa0692050:five_prime_UTR 30.0%
! ATCCTTTTCCTAATTTCATC+AGG + chr4_4:60382563-60382582 None:intergenic 30.0%
TGATGAAATTAGGAAAAGGA+TGG - chr4_4:60382562-60382581 Msa0692050:five_prime_UTR 30.0%
! CTGAAGAATTGGTAACATAA+TGG - chr4_4:60380870-60380889 Msa0692050:three_prime_UTR 30.0%
ATAACCAATCTTATCTAGCT+GGG + chr4_4:60380897-60380916 None:intergenic 30.0%
TATAACCAATCTTATCTAGC+TGG + chr4_4:60380898-60380917 None:intergenic 30.0%
! AAGAAGTAGAAAATAATGGG+TGG - chr4_4:60381052-60381071 Msa0692050:CDS 30.0%
!! TGGTTCATTTTTCTGCTTAT+TGG - chr4_4:60381350-60381369 Msa0692050:intron 30.0%
ATGCGAATTCTTCAAAGAAA+GGG + chr4_4:60381399-60381418 None:intergenic 30.0%
!!! ATTTTTTATGGTCTATGGAG+GGG - chr4_4:60381472-60381491 Msa0692050:intron 30.0%
! AAACTGAAAGTTTAGTTCGT+TGG + chr4_4:60381642-60381661 None:intergenic 30.0%
! CAGTTCAAAAAAGTACAGTT+CGG - chr4_4:60381702-60381721 Msa0692050:intron 30.0%
TATGATATCTCCTTCTATGT+TGG - chr4_4:60381781-60381800 Msa0692050:intron 30.0%
!! TTATCATCATCCTTTTTAGG+TGG - chr4_4:60382021-60382040 Msa0692050:intron 30.0%
ATCCTGATGAAATTAGGAAA+AGG - chr4_4:60382558-60382577 Msa0692050:five_prime_UTR 30.0%
! ATCCTTTTCCTAATTTCATC+AGG + chr4_4:60382563-60382582 None:intergenic 30.0%
TGATGAAATTAGGAAAAGGA+TGG - chr4_4:60382562-60382581 Msa0692050:five_prime_UTR 30.0%
TAACCAATCTTATCTAGCTG+GGG + chr4_4:60380896-60380915 None:intergenic 35.0%
! TGAGTCAATTTTCACAACCT+TGG + chr4_4:60381098-60381117 None:intergenic 35.0%
ATCACTGAAGCTACCAATAA+AGG - chr4_4:60381139-60381158 Msa0692050:intron 35.0%
ACTTACAAGGCAACCTTTAT+TGG + chr4_4:60381155-60381174 None:intergenic 35.0%
AAAGGGTTCATAGCAATAGA+AGG + chr4_4:60381382-60381401 None:intergenic 35.0%
GATGCGAATTCTTCAAAGAA+AGG + chr4_4:60381400-60381419 None:intergenic 35.0%
!! TTTAAAATGTCGGTGTTGCA+CGG + chr4_4:60381561-60381580 None:intergenic 35.0%
!!! AGTCTCTGACATGTTTTAGT+AGG - chr4_4:60381595-60381614 Msa0692050:intron 35.0%
GTGAACTAATTGTCACAGTT+CGG - chr4_4:60381662-60381681 Msa0692050:intron 35.0%
ATCTCCTTCTATGTTGGAAA+TGG - chr4_4:60381787-60381806 Msa0692050:intron 35.0%
!!! ATCCTTTTTAGGTGGTTTCA+AGG - chr4_4:60382029-60382048 Msa0692050:intron 35.0%
!!! TCCTTTTTAGGTGGTTTCAA+GGG - chr4_4:60382030-60382049 Msa0692050:intron 35.0%
ACAACAATCATGCATCAAAG+AGG + chr4_4:60382372-60382391 None:intergenic 35.0%
CATAGAACAGTCCAAACAAA+AGG + chr4_4:60382430-60382449 None:intergenic 35.0%
!! TGCTTTTTGTGACTCTATCT+AGG - chr4_4:60382448-60382467 Msa0692050:CDS 35.0%
GTGCAAATCCTGATGAAATT+AGG - chr4_4:60382552-60382571 Msa0692050:five_prime_UTR 35.0%
TAACCAATCTTATCTAGCTG+GGG + chr4_4:60380896-60380915 None:intergenic 35.0%
! TGAGTCAATTTTCACAACCT+TGG + chr4_4:60381098-60381117 None:intergenic 35.0%
ATCACTGAAGCTACCAATAA+AGG - chr4_4:60381139-60381158 Msa0692050:intron 35.0%
ACTTACAAGGCAACCTTTAT+TGG + chr4_4:60381155-60381174 None:intergenic 35.0%
AAAGGGTTCATAGCAATAGA+AGG + chr4_4:60381382-60381401 None:intergenic 35.0%
GATGCGAATTCTTCAAAGAA+AGG + chr4_4:60381400-60381419 None:intergenic 35.0%
!! TTTAAAATGTCGGTGTTGCA+CGG + chr4_4:60381561-60381580 None:intergenic 35.0%
!!! AGTCTCTGACATGTTTTAGT+AGG - chr4_4:60381595-60381614 Msa0692050:intron 35.0%
GTGAACTAATTGTCACAGTT+CGG - chr4_4:60381662-60381681 Msa0692050:intron 35.0%
ATCTCCTTCTATGTTGGAAA+TGG - chr4_4:60381787-60381806 Msa0692050:intron 35.0%
!!! ATCCTTTTTAGGTGGTTTCA+AGG - chr4_4:60382029-60382048 Msa0692050:intron 35.0%
!!! TCCTTTTTAGGTGGTTTCAA+GGG - chr4_4:60382030-60382049 Msa0692050:intron 35.0%
ACAACAATCATGCATCAAAG+AGG + chr4_4:60382372-60382391 None:intergenic 35.0%
CATAGAACAGTCCAAACAAA+AGG + chr4_4:60382430-60382449 None:intergenic 35.0%
!! TGCTTTTTGTGACTCTATCT+AGG - chr4_4:60382448-60382467 Msa0692050:CDS 35.0%
GTGCAAATCCTGATGAAATT+AGG - chr4_4:60382552-60382571 Msa0692050:five_prime_UTR 35.0%
CATGAGCATAGCTGAAGAAT+TGG - chr4_4:60380859-60380878 Msa0692050:three_prime_UTR 40.0%
ACAACAGCACAAAAAGTCCA+AGG - chr4_4:60381078-60381097 Msa0692050:CDS 40.0%
! TATTGGTAGCTTCAGTGATG+TGG + chr4_4:60381138-60381157 None:intergenic 40.0%
GGTGATTATGGTTTCAGCTT+CGG - chr4_4:60381243-60381262 Msa0692050:intron 40.0%
AAAGAGGACATCTTGATAGG+TGG + chr4_4:60381422-60381441 None:intergenic 40.0%
CAGAAAGAGGACATCTTGAT+AGG + chr4_4:60381425-60381444 None:intergenic 40.0%
TCAAGATGTCCTCTTTCTGA+AGG - chr4_4:60381426-60381445 Msa0692050:intron 40.0%
! TACAGTTCGGTTTTCTGTGA+AGG - chr4_4:60381715-60381734 Msa0692050:intron 40.0%
!! CTGTGAAGGTAGTTCTGATT+CGG - chr4_4:60381729-60381748 Msa0692050:intron 40.0%
TTGCATGTGCTGCAAATTAG+AGG + chr4_4:60381815-60381834 None:intergenic 40.0%
AAGCATGCTATCACTAAGAC+TGG - chr4_4:60381921-60381940 Msa0692050:intron 40.0%
! TGCTATCACTAAGACTGGTT+TGG - chr4_4:60381926-60381945 Msa0692050:intron 40.0%
TCCCTTGAAACCACCTAAAA+AGG + chr4_4:60382034-60382053 None:intergenic 40.0%
GTTGAGTGAAGAGACTCTTA+TGG - chr4_4:60382142-60382161 Msa0692050:CDS 40.0%
ATAAACACAGCCCTCAAAGT+GGG + chr4_4:60382196-60382215 None:intergenic 40.0%
TATAAACACAGCCCTCAAAG+TGG + chr4_4:60382197-60382216 None:intergenic 40.0%
!! CATGGCATTGATTTCCAACT+TGG + chr4_4:60382483-60382502 None:intergenic 40.0%
CATCAACAAAAAGGTAGGCA+TGG + chr4_4:60382501-60382520 None:intergenic 40.0%
CCACTCATCAACAAAAAGGT+AGG + chr4_4:60382506-60382525 None:intergenic 40.0%
!! CCTACCTTTTTGTTGATGAG+TGG - chr4_4:60382503-60382522 Msa0692050:CDS 40.0%
!! ACCTTTTTGTTGATGAGTGG+AGG - chr4_4:60382506-60382525 Msa0692050:CDS 40.0%
TCCTCCACTCATCAACAAAA+AGG + chr4_4:60382510-60382529 None:intergenic 40.0%
! GTGATTTGGAGGAATGAGTT+TGG + chr4_4:60382601-60382620 None:intergenic 40.0%
ATTCCTCCAAATCACTCTGA+TGG - chr4_4:60382606-60382625 Msa0692050:five_prime_UTR 40.0%
CATCATCCATCAGAGTGATT+TGG + chr4_4:60382615-60382634 None:intergenic 40.0%
CATGAGCATAGCTGAAGAAT+TGG - chr4_4:60380859-60380878 Msa0692050:three_prime_UTR 40.0%
ACAACAGCACAAAAAGTCCA+AGG - chr4_4:60381078-60381097 Msa0692050:CDS 40.0%
! TATTGGTAGCTTCAGTGATG+TGG + chr4_4:60381138-60381157 None:intergenic 40.0%
GGTGATTATGGTTTCAGCTT+CGG - chr4_4:60381243-60381262 Msa0692050:intron 40.0%
AAAGAGGACATCTTGATAGG+TGG + chr4_4:60381422-60381441 None:intergenic 40.0%
CAGAAAGAGGACATCTTGAT+AGG + chr4_4:60381425-60381444 None:intergenic 40.0%
TCAAGATGTCCTCTTTCTGA+AGG - chr4_4:60381426-60381445 Msa0692050:intron 40.0%
! TACAGTTCGGTTTTCTGTGA+AGG - chr4_4:60381715-60381734 Msa0692050:intron 40.0%
!! CTGTGAAGGTAGTTCTGATT+CGG - chr4_4:60381729-60381748 Msa0692050:intron 40.0%
TTGCATGTGCTGCAAATTAG+AGG + chr4_4:60381815-60381834 None:intergenic 40.0%
AAGCATGCTATCACTAAGAC+TGG - chr4_4:60381921-60381940 Msa0692050:intron 40.0%
! TGCTATCACTAAGACTGGTT+TGG - chr4_4:60381926-60381945 Msa0692050:intron 40.0%
TCCCTTGAAACCACCTAAAA+AGG + chr4_4:60382034-60382053 None:intergenic 40.0%
GTTGAGTGAAGAGACTCTTA+TGG - chr4_4:60382142-60382161 Msa0692050:CDS 40.0%
ATAAACACAGCCCTCAAAGT+GGG + chr4_4:60382196-60382215 None:intergenic 40.0%
TATAAACACAGCCCTCAAAG+TGG + chr4_4:60382197-60382216 None:intergenic 40.0%
!! CATGGCATTGATTTCCAACT+TGG + chr4_4:60382483-60382502 None:intergenic 40.0%
CATCAACAAAAAGGTAGGCA+TGG + chr4_4:60382501-60382520 None:intergenic 40.0%
CCACTCATCAACAAAAAGGT+AGG + chr4_4:60382506-60382525 None:intergenic 40.0%
!! CCTACCTTTTTGTTGATGAG+TGG - chr4_4:60382503-60382522 Msa0692050:CDS 40.0%
!! ACCTTTTTGTTGATGAGTGG+AGG - chr4_4:60382506-60382525 Msa0692050:CDS 40.0%
TCCTCCACTCATCAACAAAA+AGG + chr4_4:60382510-60382529 None:intergenic 40.0%
! GTGATTTGGAGGAATGAGTT+TGG + chr4_4:60382601-60382620 None:intergenic 40.0%
ATTCCTCCAAATCACTCTGA+TGG - chr4_4:60382606-60382625 Msa0692050:five_prime_UTR 40.0%
CATCATCCATCAGAGTGATT+TGG + chr4_4:60382615-60382634 None:intergenic 40.0%
! AGATGTCCTCTTTCTGAAGG+TGG - chr4_4:60381429-60381448 Msa0692050:intron 45.0%
TTTATGGTCTATGGAGGGGA+AGG - chr4_4:60381476-60381495 Msa0692050:intron 45.0%
!! AGGTAGTTCTGATTCGGTTC+AGG - chr4_4:60381735-60381754 Msa0692050:intron 45.0%
GGCTCCATTTCCAACATAGA+AGG + chr4_4:60381794-60381813 None:intergenic 45.0%
TGATTCCAAACCCCACTTTG+AGG - chr4_4:60381867-60381886 Msa0692050:intron 45.0%
AGTTCCTGTGACCTCAAAGT+GGG + chr4_4:60381881-60381900 None:intergenic 45.0%
GATTCCAAACCCCACTTTGA+GGG - chr4_4:60381868-60381887 Msa0692050:intron 45.0%
TAAACACAGCCCTCAAAGTG+GGG + chr4_4:60382195-60382214 None:intergenic 45.0%
!! GGCATTGATTTCCAACTTGG+AGG + chr4_4:60382480-60382499 None:intergenic 45.0%
AATCCAGTGGAGAAAGCTGT+TGG - chr4_4:60382530-60382549 Msa0692050:five_prime_UTR 45.0%
! CATCCATCAGAGTGATTTGG+AGG + chr4_4:60382612-60382631 None:intergenic 45.0%
! AGATGTCCTCTTTCTGAAGG+TGG - chr4_4:60381429-60381448 Msa0692050:intron 45.0%
TTTATGGTCTATGGAGGGGA+AGG - chr4_4:60381476-60381495 Msa0692050:intron 45.0%
!! AGGTAGTTCTGATTCGGTTC+AGG - chr4_4:60381735-60381754 Msa0692050:intron 45.0%
GGCTCCATTTCCAACATAGA+AGG + chr4_4:60381794-60381813 None:intergenic 45.0%
TGATTCCAAACCCCACTTTG+AGG - chr4_4:60381867-60381886 Msa0692050:intron 45.0%
AGTTCCTGTGACCTCAAAGT+GGG + chr4_4:60381881-60381900 None:intergenic 45.0%
GATTCCAAACCCCACTTTGA+GGG - chr4_4:60381868-60381887 Msa0692050:intron 45.0%
TAAACACAGCCCTCAAAGTG+GGG + chr4_4:60382195-60382214 None:intergenic 45.0%
!! GGCATTGATTTCCAACTTGG+AGG + chr4_4:60382480-60382499 None:intergenic 45.0%
AATCCAGTGGAGAAAGCTGT+TGG - chr4_4:60382530-60382549 Msa0692050:five_prime_UTR 45.0%
! CATCCATCAGAGTGATTTGG+AGG + chr4_4:60382612-60382631 None:intergenic 45.0%
TGGCCCCAGCTAGATAAGAT+TGG - chr4_4:60380890-60380909 Msa0692050:three_prime_UTR 50.0%
CCACATCCACCTTCAGAAAG+AGG + chr4_4:60381438-60381457 None:intergenic 50.0%
! CCTCTTTCTGAAGGTGGATG+TGG - chr4_4:60381435-60381454 Msa0692050:intron 50.0%
GGTGGATGTGGACGAAGTTA+AGG - chr4_4:60381447-60381466 Msa0692050:intron 50.0%
!! TGTCGGTGTTGCACGGATAA+TGG + chr4_4:60381554-60381573 None:intergenic 50.0%
TGTGACCTCAAAGTGGGGTT+TGG + chr4_4:60381875-60381894 None:intergenic 50.0%
!! AAACCCCACTTTGAGGTCAC+AGG - chr4_4:60381874-60381893 Msa0692050:intron 50.0%
GTTCCTGTGACCTCAAAGTG+GGG + chr4_4:60381880-60381899 None:intergenic 50.0%
CAGTTCCTGTGACCTCAAAG+TGG + chr4_4:60381882-60381901 None:intergenic 50.0%
GGATTCCAAACCCCACTTTG+AGG - chr4_4:60382182-60382201 Msa0692050:CDS 50.0%
CTAGGAAGTAGCCTCCAAGT+TGG - chr4_4:60382466-60382485 Msa0692050:CDS 50.0%
GATGAGTGGAGGAAATCCAG+TGG - chr4_4:60382517-60382536 Msa0692050:exon 50.0%
TGGCCCCAGCTAGATAAGAT+TGG - chr4_4:60380890-60380909 Msa0692050:three_prime_UTR 50.0%
CCACATCCACCTTCAGAAAG+AGG + chr4_4:60381438-60381457 None:intergenic 50.0%
! CCTCTTTCTGAAGGTGGATG+TGG - chr4_4:60381435-60381454 Msa0692050:intron 50.0%
GGTGGATGTGGACGAAGTTA+AGG - chr4_4:60381447-60381466 Msa0692050:intron 50.0%
!! TGTCGGTGTTGCACGGATAA+TGG + chr4_4:60381554-60381573 None:intergenic 50.0%
TGTGACCTCAAAGTGGGGTT+TGG + chr4_4:60381875-60381894 None:intergenic 50.0%
!! AAACCCCACTTTGAGGTCAC+AGG - chr4_4:60381874-60381893 Msa0692050:intron 50.0%
GTTCCTGTGACCTCAAAGTG+GGG + chr4_4:60381880-60381899 None:intergenic 50.0%
CAGTTCCTGTGACCTCAAAG+TGG + chr4_4:60381882-60381901 None:intergenic 50.0%
GGATTCCAAACCCCACTTTG+AGG - chr4_4:60382182-60382201 Msa0692050:CDS 50.0%
CTAGGAAGTAGCCTCCAAGT+TGG - chr4_4:60382466-60382485 Msa0692050:CDS 50.0%
GATGAGTGGAGGAAATCCAG+TGG - chr4_4:60382517-60382536 Msa0692050:exon 50.0%
ACAGCCCTCAAAGTGGGGTT+TGG + chr4_4:60382190-60382209 None:intergenic 55.0%
GCACCAACAGCTTTCTCCAC+TGG + chr4_4:60382536-60382555 None:intergenic 55.0%
ACAGCCCTCAAAGTGGGGTT+TGG + chr4_4:60382190-60382209 None:intergenic 55.0%
GCACCAACAGCTTTCTCCAC+TGG + chr4_4:60382536-60382555 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 60380857 60382737 60380857 ID=Msa0692050;Name=Msa0692050
chr4_4 mRNA 60380857 60382737 60380857 ID=Msa0692050-mRNA-1;Parent=Msa0692050;Name=Msa0692050-mRNA-1;_AED=0.15;_eAED=0.15;_QI=210|1|1|1|1|1|3|112|126
chr4_4 exon 60380857 60381124 60380857 ID=Msa0692050-mRNA-1:exon:16548;Parent=Msa0692050-mRNA-1
chr4_4 exon 60382126 60382248 60382126 ID=Msa0692050-mRNA-1:exon:16547;Parent=Msa0692050-mRNA-1
chr4_4 exon 60382426 60382737 60382426 ID=Msa0692050-mRNA-1:exon:16546;Parent=Msa0692050-mRNA-1
chr4_4 five_prime_UTR 60382528 60382737 60382528 ID=Msa0692050-mRNA-1:five_prime_utr;Parent=Msa0692050-mRNA-1
chr4_4 CDS 60382426 60382527 60382426 ID=Msa0692050-mRNA-1:cds;Parent=Msa0692050-mRNA-1
chr4_4 CDS 60382126 60382248 60382126 ID=Msa0692050-mRNA-1:cds;Parent=Msa0692050-mRNA-1
chr4_4 CDS 60380969 60381124 60380969 ID=Msa0692050-mRNA-1:cds;Parent=Msa0692050-mRNA-1
chr4_4 three_prime_UTR 60380857 60380968 60380857 ID=Msa0692050-mRNA-1:three_prime_utr;Parent=Msa0692050-mRNA-1
Gene Sequence

>Msa0692050

ATGGGTGGTCAACAACAGCACAAAAAGTCCAAGGTTGTGAAAATTGACTCAAGAAAATCATGGGAACACCACATCACTGAAGCTACCAATAAAGGTTGCCTTGTTATGGTTCATTTTTCTGCTTATTGGTGCGTTCCTTCTATTGCTATGAACCCTTTCTTTGAAGAATTCGCATCCACCTATCAAGATGTCCTCTTTCTGAAGGTGGATGTGGACGAAGTTAAGGAAGTAGCCTCCAAGTTGGAAATCAATGCCATGCCTACCTTTTTGTTGATGAGTGGAGGAAATCCAGTGGAGAAAGCTGTTGGTGCAAATCCTGATGAAATTAGGAAAAGGATGGATCATTTTATTAACCAAACTCATTCCTCCAAATCACTCTGA
Protein sequence

>Msa0692050

MGGQQQHKKSKVVKIDSRKSWEHHITEATNKGCLVMVHFSAYWCVPSIAMNPFFEEFASTYQDVLFLKVDVDEVKEVASKLEINAMPTFLLMSGGNPVEKAVGANPDEIRKRMDHFINQTHSSKSL*