AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1370080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1370080 MtrunA17_Chr4g0042051 95.238 126 6 0 1 126 1 126 2.31e-87 249
Msa1370080 MtrunA17_Chr2g0319291 70.492 122 36 0 1 122 1 122 1.28e-62 187
Msa1370080 MtrunA17_Chr2g0280411 59.322 118 48 0 6 123 5 122 1.38e-49 154
Msa1370080 MtrunA17_Chr7g0216481 41.237 97 57 0 17 113 33 129 3.48e-25 92.8
Msa1370080 MtrunA17_Chr7g0245141 35.294 102 66 0 12 113 26 127 1.00e-22 86.7
Msa1370080 MtrunA17_Chr1g0155721 36.893 103 65 0 8 110 4 106 4.24e-22 84.3
Msa1370080 MtrunA17_Chr3g0142051 35.455 110 67 1 1 110 1 106 8.75e-22 83.6
Msa1370080 MtrunA17_Chr8g0334481 34.343 99 65 0 15 113 31 129 2.52e-21 82.8
Msa1370080 MtrunA17_Chr5g0404831 36.449 107 68 0 8 114 6 112 3.06e-21 82.0
Msa1370080 MtrunA17_Chr5g0415771 38.938 113 65 2 4 114 13 123 1.84e-19 77.8
Msa1370080 MtrunA17_Chr8g0393201 32.075 106 72 0 8 113 225 330 1.15e-18 79.7
Msa1370080 MtrunA17_Chr5g0415761 33.333 105 70 0 10 114 20 124 4.91e-16 68.9
Msa1370080 MtrunA17_Chr5g0415741 34.286 105 69 0 10 114 18 122 1.26e-15 67.8
Msa1370080 MtrunA17_Chr1g0201781 33.721 86 55 2 35 118 81 166 2.36e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1370080 AT1G11530.1 58.036 112 47 0 10 121 2 113 1.49e-48 151
Msa1370080 AT3G51030.1 39.623 106 64 0 8 113 4 109 4.82e-24 89.4
Msa1370080 AT3G08710.2 37.615 109 68 0 15 123 28 136 1.27e-23 89.4
Msa1370080 AT3G08710.3 37.615 109 68 0 15 123 28 136 1.27e-23 89.4
Msa1370080 AT3G08710.1 37.615 109 68 0 15 123 28 136 1.27e-23 89.4
Msa1370080 AT5G39950.1 37.037 108 68 0 6 113 21 128 1.59e-23 89.0
Msa1370080 AT3G56420.1 38.889 108 66 0 5 112 35 142 1.74e-23 89.4
Msa1370080 AT3G56420.2 38.889 108 66 0 5 112 35 142 1.74e-23 89.4
Msa1370080 AT3G56420.3 38.889 108 66 0 5 112 35 142 1.74e-23 89.4
Msa1370080 AT1G45145.1 34.286 105 69 0 9 113 4 108 7.06e-23 86.7
Msa1370080 AT2G40790.2 36.538 104 66 0 9 112 39 142 1.13e-22 87.0
Msa1370080 AT2G40790.1 36.538 104 66 0 9 112 39 142 1.13e-22 87.0
Msa1370080 AT1G59730.1 34.615 104 68 0 12 115 23 126 1.39e-21 83.6
Msa1370080 AT5G42980.1 34.906 106 69 0 9 114 4 109 1.70e-19 78.2
Msa1370080 AT1G19730.1 31.776 107 72 1 8 113 4 110 1.01e-18 76.3
Msa1370080 AT1G69880.1 29.524 105 74 0 12 116 39 143 3.20e-17 73.2
Msa1370080 AT3G17880.2 34.211 114 71 1 11 124 264 373 6.06e-16 72.8
Msa1370080 AT3G17880.1 34.211 114 71 1 11 124 271 380 7.06e-16 72.8
Msa1370080 AT2G35010.2 30.841 107 72 1 12 116 86 192 2.66e-12 61.2
Msa1370080 AT2G35010.1 30.841 107 72 1 12 116 86 192 2.66e-12 61.2

Find 43 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCTAATTGCAACTTAATT+TGG 0.183348 tig0019015:-182451 Msa1370080:five_prime_UTR
CAATTGTTGTCTTATTTATA+AGG 0.224668 tig0019015:-182341 Msa1370080:five_prime_UTR
ACTTACAAGGCAACCTTTAT+TGG 0.239798 tig0019015:+182125 None:intergenic
TTTGAAGAAGTAGAAAATAA+TGG 0.298106 tig0019015:-182229 Msa1370080:exon
GTGCAAATCCTGATGAAATT+AGG 0.320699 tig0019015:-180559 Msa1370080:CDS
TCCTCCACTCATCAACAAAA+AGG 0.360970 tig0019015:+180604 None:intergenic
ATCACTGAAGCTACCAATAA+AGG 0.364334 tig0019015:-182138 Msa1370080:CDS
TTGAAGAAGTAGAAAATAAT+GGG 0.367571 tig0019015:-182228 Msa1370080:exon
GTGATTTGGAGGAATGAGTT+TGG 0.374475 tig0019015:+180513 None:intergenic
CATGGCATTGATTTCCAACT+TGG 0.380007 tig0019015:+180631 None:intergenic
CATGAGCATAGCTGAAGAAT+TGG 0.386956 tig0019015:-182417 Msa1370080:five_prime_UTR
CATCATCCATCAGAGTGATT+TGG 0.391841 tig0019015:+180499 None:intergenic
GGTGGATGTGGACGAAGTTA+AGG 0.395797 tig0019015:-181848 Msa1370080:intron
TATAACCAATCTTATCTAGC+TGG 0.426879 tig0019015:+182381 None:intergenic
CTGAAGAATTGGTAACATAA+TGG 0.428650 tig0019015:-182406 Msa1370080:five_prime_UTR
GATGCTAATTCTTCAAAGAA+AGG 0.438089 tig0019015:+181898 None:intergenic
GGCATTGATTTCCAACTTGG+AGG 0.441850 tig0019015:+180634 None:intergenic
CAGAAAGAGGACATCTTGAT+AGG 0.447661 tig0019015:+181873 None:intergenic
AAAGGGTTCATAGCAATAGA+AGG 0.450955 tig0019015:+181916 None:intergenic
CCTCTTTCTGAAGGTGGATG+TGG 0.473122 tig0019015:-181860 Msa1370080:CDS
ATAACCAATCTTATCTAGCT+GGG 0.488311 tig0019015:+182382 None:intergenic
GCACCAACAGCTTTCTCCAC+TGG 0.488674 tig0019015:+180578 None:intergenic
ATGCTAATTCTTCAAAGAAA+GGG 0.500184 tig0019015:+181899 None:intergenic
TTGTGTATATATACAAACTC+TGG 0.503825 tig0019015:+182260 None:intergenic
CCACATCCACCTTCAGAAAG+AGG 0.505638 tig0019015:+181860 None:intergenic
ATCCTGATGAAATTAGGAAA+AGG 0.511549 tig0019015:-180553 Msa1370080:CDS
CATCCATCAGAGTGATTTGG+AGG 0.522008 tig0019015:+180502 None:intergenic
ATTCCTCCAAATCACTCTGA+TGG 0.522941 tig0019015:-180505 Msa1370080:exon
TGGCCCCAGCTAGATAAGAT+TGG 0.526897 tig0019015:-182386 Msa1370080:five_prime_UTR
ACAGTATATATAGTTTGTGT+AGG 0.531357 tig0019015:-182299 Msa1370080:five_prime_UTR
AAATTGACTCAAGAAAATCA+TGG 0.550454 tig0019015:-182170 Msa1370080:CDS
AATTGACTCAAGAAAATCAT+GGG 0.553360 tig0019015:-182169 Msa1370080:CDS
TATTGGTAGCTTCAGTGATG+TGG 0.558401 tig0019015:+182142 None:intergenic
TCAAGATGTCCTCTTTCTGA+AGG 0.562276 tig0019015:-181869 Msa1370080:CDS
ATTCTTCAGCTATGCTCATG+TGG 0.565681 tig0019015:+182420 None:intergenic
AGATGTCCTCTTTCTGAAGG+TGG 0.570239 tig0019015:-181866 Msa1370080:CDS
TGATGAAATTAGGAAAAGGA+TGG 0.577508 tig0019015:-180549 Msa1370080:CDS
TAGAATATATATTATTACAC+AGG 0.582812 tig0019015:+180389 None:intergenic
CTAGGAAGTAGCCTCCAAGT+TGG 0.602024 tig0019015:-180645 Msa1370080:intron
AATCCAGTGGAGAAAGCTGT+TGG 0.602305 tig0019015:-180581 Msa1370080:CDS
AAAGAGGACATCTTGATAGG+TGG 0.610226 tig0019015:+181876 None:intergenic
TAACCAATCTTATCTAGCTG+GGG 0.641666 tig0019015:+182383 None:intergenic
GATGAGTGGAGGAAATCCAG+TGG 0.727295 tig0019015:-180594 Msa1370080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTATATAGTTATATTAAT+AGG - tig0019015:180928-180947 Msa1370080:intron 10.0%
!! TAAACTAATCTAAATAAAAA+AGG - tig0019015:181874-181893 Msa1370080:CDS 10.0%
!! AAACTAATCTAAATAAAAAA+GGG - tig0019015:181875-181894 Msa1370080:CDS 10.0%
!! ATTACATAACATATTATTAT+TGG + tig0019015:182168-182187 None:intergenic 10.0%
!! ATCTATATAGTTATATTAAT+AGG - tig0019015:180928-180947 Msa1370080:intron 10.0%
!! TAAACTAATCTAAATAAAAA+AGG - tig0019015:181874-181893 Msa1370080:CDS 10.0%
!! AAACTAATCTAAATAAAAAA+GGG - tig0019015:181875-181894 Msa1370080:CDS 10.0%
!! ATTACATAACATATTATTAT+TGG + tig0019015:182168-182187 None:intergenic 10.0%
!! CATGTAAAATTATAACAATT+GGG - tig0019015:180875-180894 Msa1370080:intron 15.0%
!!! AAGTTAAGGTAATTTTTTTA+TGG - tig0019015:181096-181115 Msa1370080:intron 15.0%
!! TTTAAAAACACAAATGAAAA+AGG + tig0019015:181142-181161 None:intergenic 15.0%
!! AATAAACAATTTGAGAATAT+TGG + tig0019015:181476-181495 None:intergenic 15.0%
!! ACAAATTATATTGAAACAAA+AGG + tig0019015:181727-181746 None:intergenic 15.0%
!!! AATATAATTTGTGAATTTTC+TGG - tig0019015:181735-181754 Msa1370080:intron 15.0%
!! CATGTAAAATTATAACAATT+GGG - tig0019015:180875-180894 Msa1370080:intron 15.0%
!!! AAGTTAAGGTAATTTTTTTA+TGG - tig0019015:181096-181115 Msa1370080:intron 15.0%
!! TTTAAAAACACAAATGAAAA+AGG + tig0019015:181142-181161 None:intergenic 15.0%
!! AATAAACAATTTGAGAATAT+TGG + tig0019015:181476-181495 None:intergenic 15.0%
!! ACAAATTATATTGAAACAAA+AGG + tig0019015:181727-181746 None:intergenic 15.0%
!!! AATATAATTTGTGAATTTTC+TGG - tig0019015:181735-181754 Msa1370080:intron 15.0%
!!! CAATTGTTGTCTTATTTATA+AGG - tig0019015:180589-180608 Msa1370080:CDS 20.0%
!!! TATTTTCTACTTCTTCAAAA+GGG + tig0019015:180700-180719 None:intergenic 20.0%
!!! TTATTTTCTACTTCTTCAAA+AGG + tig0019015:180701-180720 None:intergenic 20.0%
!! TTTGAAGAAGTAGAAAATAA+TGG - tig0019015:180701-180720 Msa1370080:intron 20.0%
!! TTGAAGAAGTAGAAAATAAT+GGG - tig0019015:180702-180721 Msa1370080:intron 20.0%
!!! TAAAATTGATGATACTTACA+AGG + tig0019015:180821-180840 None:intergenic 20.0%
!!! TGTAAGTATCATCAATTTTA+TGG - tig0019015:180821-180840 Msa1370080:intron 20.0%
!! GCATGTAAAATTATAACAAT+TGG - tig0019015:180874-180893 Msa1370080:intron 20.0%
!! TTTGTTCATAAAATATTTGC+AGG - tig0019015:180959-180978 Msa1370080:intron 20.0%
!! ATGCCATTATTATAAGTTAA+AGG + tig0019015:181586-181605 None:intergenic 20.0%
!! AATTATATTGAAACAAAAGG+TGG + tig0019015:181724-181743 None:intergenic 20.0%
!!! ATTAGTTTAGAAAATAAGCA+TGG + tig0019015:181863-181882 None:intergenic 20.0%
!! AACTTGTGTATTTAATACAT+TGG - tig0019015:181959-181978 Msa1370080:intron 20.0%
!!! CCTACATGTATTTTTTTATT+GGG + tig0019015:182069-182088 None:intergenic 20.0%
!! CCAATAAAAAAATACATGTA+GGG - tig0019015:182067-182086 Msa1370080:intron 20.0%
!!! CAATTGTTGTCTTATTTATA+AGG - tig0019015:180589-180608 Msa1370080:CDS 20.0%
!!! TATTTTCTACTTCTTCAAAA+GGG + tig0019015:180700-180719 None:intergenic 20.0%
!!! TTATTTTCTACTTCTTCAAA+AGG + tig0019015:180701-180720 None:intergenic 20.0%
!! TTTGAAGAAGTAGAAAATAA+TGG - tig0019015:180701-180720 Msa1370080:intron 20.0%
!! TTGAAGAAGTAGAAAATAAT+GGG - tig0019015:180702-180721 Msa1370080:intron 20.0%
!!! TAAAATTGATGATACTTACA+AGG + tig0019015:180821-180840 None:intergenic 20.0%
!!! TGTAAGTATCATCAATTTTA+TGG - tig0019015:180821-180840 Msa1370080:intron 20.0%
!! GCATGTAAAATTATAACAAT+TGG - tig0019015:180874-180893 Msa1370080:intron 20.0%
!! TTTGTTCATAAAATATTTGC+AGG - tig0019015:180959-180978 Msa1370080:intron 20.0%
!! ATGCCATTATTATAAGTTAA+AGG + tig0019015:181586-181605 None:intergenic 20.0%
!! AATTATATTGAAACAAAAGG+TGG + tig0019015:181724-181743 None:intergenic 20.0%
!!! ATTAGTTTAGAAAATAAGCA+TGG + tig0019015:181863-181882 None:intergenic 20.0%
!! AACTTGTGTATTTAATACAT+TGG - tig0019015:181959-181978 Msa1370080:intron 20.0%
!!! CCTACATGTATTTTTTTATT+GGG + tig0019015:182069-182088 None:intergenic 20.0%
!! CCAATAAAAAAATACATGTA+GGG - tig0019015:182067-182086 Msa1370080:intron 20.0%
! TGTCTAATTGCAACTTAATT+TGG - tig0019015:180479-180498 Msa1370080:three_prime_UTR 25.0%
!! ACAGTATATATAGTTTGTGT+AGG - tig0019015:180631-180650 Msa1370080:CDS 25.0%
! TTGTGTATATATACAAACTC+TGG + tig0019015:180673-180692 None:intergenic 25.0%
!! AAATTGACTCAAGAAAATCA+TGG - tig0019015:180760-180779 Msa1370080:intron 25.0%
!! AATTGACTCAAGAAAATCAT+GGG - tig0019015:180761-180780 Msa1370080:intron 25.0%
! TTATAACAATTGGGTGATTA+TGG - tig0019015:180884-180903 Msa1370080:intron 25.0%
! CATAAAATATTTGCAGGTTA+TGG - tig0019015:180965-180984 Msa1370080:intron 25.0%
! ATGCTAATTCTTCAAAGAAA+GGG + tig0019015:181034-181053 None:intergenic 25.0%
!!! GGTAATTTTTTTATGGTCTA+TGG - tig0019015:181103-181122 Msa1370080:intron 25.0%
!!! AATTTTTTTATGGTCTATGG+AGG - tig0019015:181106-181125 Msa1370080:intron 25.0%
!!! ATTTTTTTATGGTCTATGGA+GGG - tig0019015:181107-181126 Msa1370080:intron 25.0%
!! ACGCTTTCAATTTAAAATGT+CGG + tig0019015:181203-181222 None:intergenic 25.0%
! AAACTAACATGTAGTTTCTT+TGG + tig0019015:181394-181413 None:intergenic 25.0%
!! GAGCCTTTAACTTATAATAA+TGG - tig0019015:181580-181599 Msa1370080:intron 25.0%
! TTGTAGATGATATCAATATG+TGG - tig0019015:181614-181633 Msa1370080:intron 25.0%
!! CTTTTATCATCATCCTTTTT+AGG - tig0019015:181654-181673 Msa1370080:intron 25.0%
!! ATGGAAGAAACTGTTTATTT+TGG - tig0019015:181797-181816 Msa1370080:intron 25.0%
!!! CTCTTTAAGAGTGTTAAATT+AGG + tig0019015:182020-182039 None:intergenic 25.0%
! CCCAATAAAAAAATACATGT+AGG - tig0019015:182066-182085 Msa1370080:intron 25.0%
!!! CCCTACATGTATTTTTTTAT+TGG + tig0019015:182070-182089 None:intergenic 25.0%
!! TTAACTAACTACCTTTTGTT+TGG - tig0019015:182235-182254 Msa1370080:five_prime_UTR 25.0%
! TGTCTAATTGCAACTTAATT+TGG - tig0019015:180479-180498 Msa1370080:three_prime_UTR 25.0%
!! ACAGTATATATAGTTTGTGT+AGG - tig0019015:180631-180650 Msa1370080:CDS 25.0%
! TTGTGTATATATACAAACTC+TGG + tig0019015:180673-180692 None:intergenic 25.0%
!! AAATTGACTCAAGAAAATCA+TGG - tig0019015:180760-180779 Msa1370080:intron 25.0%
!! AATTGACTCAAGAAAATCAT+GGG - tig0019015:180761-180780 Msa1370080:intron 25.0%
! TTATAACAATTGGGTGATTA+TGG - tig0019015:180884-180903 Msa1370080:intron 25.0%
! CATAAAATATTTGCAGGTTA+TGG - tig0019015:180965-180984 Msa1370080:intron 25.0%
! ATGCTAATTCTTCAAAGAAA+GGG + tig0019015:181034-181053 None:intergenic 25.0%
!!! GGTAATTTTTTTATGGTCTA+TGG - tig0019015:181103-181122 Msa1370080:intron 25.0%
!!! AATTTTTTTATGGTCTATGG+AGG - tig0019015:181106-181125 Msa1370080:intron 25.0%
!!! ATTTTTTTATGGTCTATGGA+GGG - tig0019015:181107-181126 Msa1370080:intron 25.0%
!! ACGCTTTCAATTTAAAATGT+CGG + tig0019015:181203-181222 None:intergenic 25.0%
! AAACTAACATGTAGTTTCTT+TGG + tig0019015:181394-181413 None:intergenic 25.0%
!! GAGCCTTTAACTTATAATAA+TGG - tig0019015:181580-181599 Msa1370080:intron 25.0%
! TTGTAGATGATATCAATATG+TGG - tig0019015:181614-181633 Msa1370080:intron 25.0%
!! CTTTTATCATCATCCTTTTT+AGG - tig0019015:181654-181673 Msa1370080:intron 25.0%
!! ATGGAAGAAACTGTTTATTT+TGG - tig0019015:181797-181816 Msa1370080:intron 25.0%
!!! CTCTTTAAGAGTGTTAAATT+AGG + tig0019015:182020-182039 None:intergenic 25.0%
! CCCAATAAAAAAATACATGT+AGG - tig0019015:182066-182085 Msa1370080:intron 25.0%
!!! CCCTACATGTATTTTTTTAT+TGG + tig0019015:182070-182089 None:intergenic 25.0%
!! TTAACTAACTACCTTTTGTT+TGG - tig0019015:182235-182254 Msa1370080:five_prime_UTR 25.0%
! CTGAAGAATTGGTAACATAA+TGG - tig0019015:180524-180543 Msa1370080:CDS 30.0%
ATAACCAATCTTATCTAGCT+GGG + tig0019015:180551-180570 None:intergenic 30.0%
TATAACCAATCTTATCTAGC+TGG + tig0019015:180552-180571 None:intergenic 30.0%
!! TGGTTCATTTTTCTGCTTAT+TGG - tig0019015:180985-181004 Msa1370080:intron 30.0%
GATGCTAATTCTTCAAAGAA+AGG + tig0019015:181035-181054 None:intergenic 30.0%
!!! TTTTTTTATGGTCTATGGAG+GGG - tig0019015:181108-181127 Msa1370080:intron 30.0%
! AAACTGAAAGTTTAGTTCGT+TGG + tig0019015:181274-181293 None:intergenic 30.0%
! CAGTTCAAAAAAGTACAGTT+CGG - tig0019015:181334-181353 Msa1370080:intron 30.0%
TATGATATCTCCTTCTATGT+TGG - tig0019015:181413-181432 Msa1370080:intron 30.0%
!! TTATCATCATCCTTTTTAGG+TGG - tig0019015:181657-181676 Msa1370080:intron 30.0%
! ATGCCAATTTTTATGTTGAG+TGG - tig0019015:181764-181783 Msa1370080:intron 30.0%
TCTCCACTCAACATAAAAAT+TGG + tig0019015:181770-181789 None:intergenic 30.0%
GTTACTATTCTAAGCTCTTA+AGG + tig0019015:181929-181948 None:intergenic 30.0%
ATCCTGATGAAATTAGGAAA+AGG - tig0019015:182377-182396 Msa1370080:five_prime_UTR 30.0%
! ATCCTTTTCCTAATTTCATC+AGG + tig0019015:182382-182401 None:intergenic 30.0%
TGATGAAATTAGGAAAAGGA+TGG - tig0019015:182381-182400 Msa1370080:five_prime_UTR 30.0%
! CTGAAGAATTGGTAACATAA+TGG - tig0019015:180524-180543 Msa1370080:CDS 30.0%
ATAACCAATCTTATCTAGCT+GGG + tig0019015:180551-180570 None:intergenic 30.0%
TATAACCAATCTTATCTAGC+TGG + tig0019015:180552-180571 None:intergenic 30.0%
!! TGGTTCATTTTTCTGCTTAT+TGG - tig0019015:180985-181004 Msa1370080:intron 30.0%
GATGCTAATTCTTCAAAGAA+AGG + tig0019015:181035-181054 None:intergenic 30.0%
!!! TTTTTTTATGGTCTATGGAG+GGG - tig0019015:181108-181127 Msa1370080:intron 30.0%
! AAACTGAAAGTTTAGTTCGT+TGG + tig0019015:181274-181293 None:intergenic 30.0%
! CAGTTCAAAAAAGTACAGTT+CGG - tig0019015:181334-181353 Msa1370080:intron 30.0%
TATGATATCTCCTTCTATGT+TGG - tig0019015:181413-181432 Msa1370080:intron 30.0%
!! TTATCATCATCCTTTTTAGG+TGG - tig0019015:181657-181676 Msa1370080:intron 30.0%
! ATGCCAATTTTTATGTTGAG+TGG - tig0019015:181764-181783 Msa1370080:intron 30.0%
TCTCCACTCAACATAAAAAT+TGG + tig0019015:181770-181789 None:intergenic 30.0%
GTTACTATTCTAAGCTCTTA+AGG + tig0019015:181929-181948 None:intergenic 30.0%
ATCCTGATGAAATTAGGAAA+AGG - tig0019015:182377-182396 Msa1370080:five_prime_UTR 30.0%
! ATCCTTTTCCTAATTTCATC+AGG + tig0019015:182382-182401 None:intergenic 30.0%
TGATGAAATTAGGAAAAGGA+TGG - tig0019015:182381-182400 Msa1370080:five_prime_UTR 30.0%
TAACCAATCTTATCTAGCTG+GGG + tig0019015:180550-180569 None:intergenic 35.0%
! TGAGTCAATTTTCACAACCT+TGG + tig0019015:180751-180770 None:intergenic 35.0%
ATCACTGAAGCTACCAATAA+AGG - tig0019015:180792-180811 Msa1370080:intron 35.0%
ACTTACAAGGCAACCTTTAT+TGG + tig0019015:180808-180827 None:intergenic 35.0%
AAAGGGTTCATAGCAATAGA+AGG + tig0019015:181017-181036 None:intergenic 35.0%
! TGCACGGATAATGGTATATA+TGG + tig0019015:181177-181196 None:intergenic 35.0%
!! TTTAAAATGTCGGTGTTGCA+CGG + tig0019015:181193-181212 None:intergenic 35.0%
!!! AGTCTCTGACATGTTTTAGT+AGG - tig0019015:181227-181246 Msa1370080:intron 35.0%
GTGAACTAATTGTCACAGTT+CGG - tig0019015:181294-181313 Msa1370080:intron 35.0%
ATCTCCTTCTATGTTGGAAA+TGG - tig0019015:181419-181438 Msa1370080:intron 35.0%
!!! ATCCTTTTTAGGTGGTTTCA+AGG - tig0019015:181665-181684 Msa1370080:intron 35.0%
!!! TCCTTTTTAGGTGGTTTCAA+GGG - tig0019015:181666-181685 Msa1370080:intron 35.0%
ACAACAATCATGCATCAAAG+AGG + tig0019015:182191-182210 None:intergenic 35.0%
CATAGAACAGTCCAAACAAA+AGG + tig0019015:182249-182268 None:intergenic 35.0%
! TGCATTTTGTGACTCTATCT+AGG - tig0019015:182267-182286 Msa1370080:five_prime_UTR 35.0%
GTGCAAATCCTGATGAAATT+AGG - tig0019015:182371-182390 Msa1370080:five_prime_UTR 35.0%
TAACCAATCTTATCTAGCTG+GGG + tig0019015:180550-180569 None:intergenic 35.0%
! TGAGTCAATTTTCACAACCT+TGG + tig0019015:180751-180770 None:intergenic 35.0%
ATCACTGAAGCTACCAATAA+AGG - tig0019015:180792-180811 Msa1370080:intron 35.0%
ACTTACAAGGCAACCTTTAT+TGG + tig0019015:180808-180827 None:intergenic 35.0%
AAAGGGTTCATAGCAATAGA+AGG + tig0019015:181017-181036 None:intergenic 35.0%
! TGCACGGATAATGGTATATA+TGG + tig0019015:181177-181196 None:intergenic 35.0%
!! TTTAAAATGTCGGTGTTGCA+CGG + tig0019015:181193-181212 None:intergenic 35.0%
!!! AGTCTCTGACATGTTTTAGT+AGG - tig0019015:181227-181246 Msa1370080:intron 35.0%
GTGAACTAATTGTCACAGTT+CGG - tig0019015:181294-181313 Msa1370080:intron 35.0%
ATCTCCTTCTATGTTGGAAA+TGG - tig0019015:181419-181438 Msa1370080:intron 35.0%
!!! ATCCTTTTTAGGTGGTTTCA+AGG - tig0019015:181665-181684 Msa1370080:intron 35.0%
!!! TCCTTTTTAGGTGGTTTCAA+GGG - tig0019015:181666-181685 Msa1370080:intron 35.0%
ACAACAATCATGCATCAAAG+AGG + tig0019015:182191-182210 None:intergenic 35.0%
CATAGAACAGTCCAAACAAA+AGG + tig0019015:182249-182268 None:intergenic 35.0%
! TGCATTTTGTGACTCTATCT+AGG - tig0019015:182267-182286 Msa1370080:five_prime_UTR 35.0%
GTGCAAATCCTGATGAAATT+AGG - tig0019015:182371-182390 Msa1370080:five_prime_UTR 35.0%
ATTCTTCAGCTATGCTCATG+TGG + tig0019015:180513-180532 None:intergenic 40.0%
CATGAGCATAGCTGAAGAAT+TGG - tig0019015:180513-180532 Msa1370080:CDS 40.0%
ACAACAGCACAAAAAGTCCA+AGG - tig0019015:180731-180750 Msa1370080:intron 40.0%
! TATTGGTAGCTTCAGTGATG+TGG + tig0019015:180791-180810 None:intergenic 40.0%
GGTGATTATGGTTTCAGCTT+CGG - tig0019015:180896-180915 Msa1370080:intron 40.0%
AAAGAGGACATCTTGATAGG+TGG + tig0019015:181057-181076 None:intergenic 40.0%
CAGAAAGAGGACATCTTGAT+AGG + tig0019015:181060-181079 None:intergenic 40.0%
TCAAGATGTCCTCTTTCTGA+AGG - tig0019015:181061-181080 Msa1370080:intron 40.0%
! TACAGTTCGGTTTTCTGTGA+AGG - tig0019015:181347-181366 Msa1370080:intron 40.0%
!! CTGTGAAGGTAGTTCTGATT+CGG - tig0019015:181361-181380 Msa1370080:intron 40.0%
TTGCATGTGCTGCAAATTAG+AGG + tig0019015:181447-181466 None:intergenic 40.0%
AAGCATGCTATCACTAAGAC+TGG - tig0019015:181553-181572 Msa1370080:intron 40.0%
! TGCTATCACTAAGACTGGTT+TGG - tig0019015:181558-181577 Msa1370080:intron 40.0%
TCCCTTGAAACCACCTAAAA+AGG + tig0019015:181670-181689 None:intergenic 40.0%
ATAAACACAGCCCTCAAAGT+GGG + tig0019015:181832-181851 None:intergenic 40.0%
TATAAACACAGCCCTCAAAG+TGG + tig0019015:181833-181852 None:intergenic 40.0%
!! CATGGCATTGATTTCCAACT+TGG + tig0019015:182302-182321 None:intergenic 40.0%
CATCAACAAAAAGGTAGGCA+TGG + tig0019015:182320-182339 None:intergenic 40.0%
CCACTCATCAACAAAAAGGT+AGG + tig0019015:182325-182344 None:intergenic 40.0%
!! CCTACCTTTTTGTTGATGAG+TGG - tig0019015:182322-182341 Msa1370080:five_prime_UTR 40.0%
!! ACCTTTTTGTTGATGAGTGG+AGG - tig0019015:182325-182344 Msa1370080:five_prime_UTR 40.0%
TCCTCCACTCATCAACAAAA+AGG + tig0019015:182329-182348 None:intergenic 40.0%
! GTGATTTGGAGGAATGAGTT+TGG + tig0019015:182420-182439 None:intergenic 40.0%
ATTCCTCCAAATCACTCTGA+TGG - tig0019015:182425-182444 Msa1370080:five_prime_UTR 40.0%
CATCATCCATCAGAGTGATT+TGG + tig0019015:182434-182453 None:intergenic 40.0%
ATTCTTCAGCTATGCTCATG+TGG + tig0019015:180513-180532 None:intergenic 40.0%
CATGAGCATAGCTGAAGAAT+TGG - tig0019015:180513-180532 Msa1370080:CDS 40.0%
ACAACAGCACAAAAAGTCCA+AGG - tig0019015:180731-180750 Msa1370080:intron 40.0%
! TATTGGTAGCTTCAGTGATG+TGG + tig0019015:180791-180810 None:intergenic 40.0%
GGTGATTATGGTTTCAGCTT+CGG - tig0019015:180896-180915 Msa1370080:intron 40.0%
AAAGAGGACATCTTGATAGG+TGG + tig0019015:181057-181076 None:intergenic 40.0%
CAGAAAGAGGACATCTTGAT+AGG + tig0019015:181060-181079 None:intergenic 40.0%
TCAAGATGTCCTCTTTCTGA+AGG - tig0019015:181061-181080 Msa1370080:intron 40.0%
! TACAGTTCGGTTTTCTGTGA+AGG - tig0019015:181347-181366 Msa1370080:intron 40.0%
!! CTGTGAAGGTAGTTCTGATT+CGG - tig0019015:181361-181380 Msa1370080:intron 40.0%
TTGCATGTGCTGCAAATTAG+AGG + tig0019015:181447-181466 None:intergenic 40.0%
AAGCATGCTATCACTAAGAC+TGG - tig0019015:181553-181572 Msa1370080:intron 40.0%
! TGCTATCACTAAGACTGGTT+TGG - tig0019015:181558-181577 Msa1370080:intron 40.0%
TCCCTTGAAACCACCTAAAA+AGG + tig0019015:181670-181689 None:intergenic 40.0%
ATAAACACAGCCCTCAAAGT+GGG + tig0019015:181832-181851 None:intergenic 40.0%
TATAAACACAGCCCTCAAAG+TGG + tig0019015:181833-181852 None:intergenic 40.0%
!! CATGGCATTGATTTCCAACT+TGG + tig0019015:182302-182321 None:intergenic 40.0%
CATCAACAAAAAGGTAGGCA+TGG + tig0019015:182320-182339 None:intergenic 40.0%
CCACTCATCAACAAAAAGGT+AGG + tig0019015:182325-182344 None:intergenic 40.0%
!! CCTACCTTTTTGTTGATGAG+TGG - tig0019015:182322-182341 Msa1370080:five_prime_UTR 40.0%
!! ACCTTTTTGTTGATGAGTGG+AGG - tig0019015:182325-182344 Msa1370080:five_prime_UTR 40.0%
TCCTCCACTCATCAACAAAA+AGG + tig0019015:182329-182348 None:intergenic 40.0%
! GTGATTTGGAGGAATGAGTT+TGG + tig0019015:182420-182439 None:intergenic 40.0%
ATTCCTCCAAATCACTCTGA+TGG - tig0019015:182425-182444 Msa1370080:five_prime_UTR 40.0%
CATCATCCATCAGAGTGATT+TGG + tig0019015:182434-182453 None:intergenic 40.0%
! AGATGTCCTCTTTCTGAAGG+TGG - tig0019015:181064-181083 Msa1370080:intron 45.0%
TTTATGGTCTATGGAGGGGA+AGG - tig0019015:181112-181131 Msa1370080:intron 45.0%
!! AGGTAGTTCTGATTCGGTTC+AGG - tig0019015:181367-181386 Msa1370080:intron 45.0%
GGCTCCATTTCCAACATAGA+AGG + tig0019015:181426-181445 None:intergenic 45.0%
TGATTCCAAACCCCACTTTG+AGG - tig0019015:181499-181518 Msa1370080:intron 45.0%
AGTTCCTGTGACCTCAAAGT+GGG + tig0019015:181513-181532 None:intergenic 45.0%
GTTGAGTGGAGAGACTCTTA+TGG - tig0019015:181778-181797 Msa1370080:intron 45.0%
GATTCCAAACCCCACTTTGA+GGG - tig0019015:181500-181519 Msa1370080:intron 45.0%
TAAACACAGCCCTCAAAGTG+GGG + tig0019015:181831-181850 None:intergenic 45.0%
!! AACACTCTTAAAGAGTGCCC+CGG - tig0019015:182025-182044 Msa1370080:intron 45.0%
!! GGCATTGATTTCCAACTTGG+AGG + tig0019015:182299-182318 None:intergenic 45.0%
AATCCAGTGGAGAAAGCTGT+TGG - tig0019015:182349-182368 Msa1370080:five_prime_UTR 45.0%
! CATCCATCAGAGTGATTTGG+AGG + tig0019015:182431-182450 None:intergenic 45.0%
! AGATGTCCTCTTTCTGAAGG+TGG - tig0019015:181064-181083 Msa1370080:intron 45.0%
TTTATGGTCTATGGAGGGGA+AGG - tig0019015:181112-181131 Msa1370080:intron 45.0%
!! AGGTAGTTCTGATTCGGTTC+AGG - tig0019015:181367-181386 Msa1370080:intron 45.0%
GGCTCCATTTCCAACATAGA+AGG + tig0019015:181426-181445 None:intergenic 45.0%
TGATTCCAAACCCCACTTTG+AGG - tig0019015:181499-181518 Msa1370080:intron 45.0%
AGTTCCTGTGACCTCAAAGT+GGG + tig0019015:181513-181532 None:intergenic 45.0%
GTTGAGTGGAGAGACTCTTA+TGG - tig0019015:181778-181797 Msa1370080:intron 45.0%
GATTCCAAACCCCACTTTGA+GGG - tig0019015:181500-181519 Msa1370080:intron 45.0%
TAAACACAGCCCTCAAAGTG+GGG + tig0019015:181831-181850 None:intergenic 45.0%
!! AACACTCTTAAAGAGTGCCC+CGG - tig0019015:182025-182044 Msa1370080:intron 45.0%
!! GGCATTGATTTCCAACTTGG+AGG + tig0019015:182299-182318 None:intergenic 45.0%
AATCCAGTGGAGAAAGCTGT+TGG - tig0019015:182349-182368 Msa1370080:five_prime_UTR 45.0%
! CATCCATCAGAGTGATTTGG+AGG + tig0019015:182431-182450 None:intergenic 45.0%
!!! TAAAAGATATTATTTTAATT+AGG + tig0019015:181640-181659 None:intergenic 5.0%
!!! TAAAAGATATTATTTTAATT+AGG + tig0019015:181640-181659 None:intergenic 5.0%
TGGCCCCAGCTAGATAAGAT+TGG - tig0019015:180544-180563 Msa1370080:CDS 50.0%
CCACATCCACCTTCAGAAAG+AGG + tig0019015:181073-181092 None:intergenic 50.0%
! CCTCTTTCTGAAGGTGGATG+TGG - tig0019015:181070-181089 Msa1370080:intron 50.0%
GGTGGATGTGGACGAAGTTA+AGG - tig0019015:181082-181101 Msa1370080:intron 50.0%
!! TGTCGGTGTTGCACGGATAA+TGG + tig0019015:181186-181205 None:intergenic 50.0%
TGTGACCTCAAAGTGGGGTT+TGG + tig0019015:181507-181526 None:intergenic 50.0%
!! AAACCCCACTTTGAGGTCAC+AGG - tig0019015:181506-181525 Msa1370080:intron 50.0%
GTTCCTGTGACCTCAAAGTG+GGG + tig0019015:181512-181531 None:intergenic 50.0%
CAGTTCCTGTGACCTCAAAG+TGG + tig0019015:181514-181533 None:intergenic 50.0%
GGATTCCAAACCCCACTTTG+AGG - tig0019015:181818-181837 Msa1370080:intron 50.0%
GAAATGTTAACGAGTGCCCC+CGG - tig0019015:181897-181916 Msa1370080:CDS 50.0%
AAATGTTAACGAGTGCCCCC+GGG - tig0019015:181898-181917 Msa1370080:CDS 50.0%
!! TAAGCTCTTAAGGAGTGCCC+CGG + tig0019015:181919-181938 None:intergenic 50.0%
!! ACACTCTTAAAGAGTGCCCC+GGG - tig0019015:182026-182045 Msa1370080:intron 50.0%
CTAGGAAGTAGCCTCCAAGT+TGG - tig0019015:182285-182304 Msa1370080:five_prime_UTR 50.0%
GATGAGTGGAGGAAATCCAG+TGG - tig0019015:182336-182355 Msa1370080:five_prime_UTR 50.0%
TGGCCCCAGCTAGATAAGAT+TGG - tig0019015:180544-180563 Msa1370080:CDS 50.0%
CCACATCCACCTTCAGAAAG+AGG + tig0019015:181073-181092 None:intergenic 50.0%
! CCTCTTTCTGAAGGTGGATG+TGG - tig0019015:181070-181089 Msa1370080:intron 50.0%
GGTGGATGTGGACGAAGTTA+AGG - tig0019015:181082-181101 Msa1370080:intron 50.0%
!! TGTCGGTGTTGCACGGATAA+TGG + tig0019015:181186-181205 None:intergenic 50.0%
TGTGACCTCAAAGTGGGGTT+TGG + tig0019015:181507-181526 None:intergenic 50.0%
!! AAACCCCACTTTGAGGTCAC+AGG - tig0019015:181506-181525 Msa1370080:intron 50.0%
GTTCCTGTGACCTCAAAGTG+GGG + tig0019015:181512-181531 None:intergenic 50.0%
CAGTTCCTGTGACCTCAAAG+TGG + tig0019015:181514-181533 None:intergenic 50.0%
GGATTCCAAACCCCACTTTG+AGG - tig0019015:181818-181837 Msa1370080:intron 50.0%
GAAATGTTAACGAGTGCCCC+CGG - tig0019015:181897-181916 Msa1370080:CDS 50.0%
AAATGTTAACGAGTGCCCCC+GGG - tig0019015:181898-181917 Msa1370080:CDS 50.0%
!! TAAGCTCTTAAGGAGTGCCC+CGG + tig0019015:181919-181938 None:intergenic 50.0%
!! ACACTCTTAAAGAGTGCCCC+GGG - tig0019015:182026-182045 Msa1370080:intron 50.0%
CTAGGAAGTAGCCTCCAAGT+TGG - tig0019015:182285-182304 Msa1370080:five_prime_UTR 50.0%
GATGAGTGGAGGAAATCCAG+TGG - tig0019015:182336-182355 Msa1370080:five_prime_UTR 50.0%
ACAGCCCTCAAAGTGGGGTT+TGG + tig0019015:181826-181845 None:intergenic 55.0%
AATGTTAACGAGTGCCCCCG+GGG - tig0019015:181899-181918 Msa1370080:CDS 55.0%
!! AAGCTCTTAAGGAGTGCCCC+GGG + tig0019015:181918-181937 None:intergenic 55.0%
!! CACTCTTAAAGAGTGCCCCG+GGG - tig0019015:182027-182046 Msa1370080:intron 55.0%
!! ACTCTTAAAGAGTGCCCCGG+GGG - tig0019015:182028-182047 Msa1370080:intron 55.0%
TCTTGTTAACGAGTGCCCCC+GGG + tig0019015:182046-182065 None:intergenic 55.0%
! GTCTTGTTAACGAGTGCCCC+CGG + tig0019015:182047-182066 None:intergenic 55.0%
GCACCAACAGCTTTCTCCAC+TGG + tig0019015:182355-182374 None:intergenic 55.0%
ACAGCCCTCAAAGTGGGGTT+TGG + tig0019015:181826-181845 None:intergenic 55.0%
AATGTTAACGAGTGCCCCCG+GGG - tig0019015:181899-181918 Msa1370080:CDS 55.0%
!! AAGCTCTTAAGGAGTGCCCC+GGG + tig0019015:181918-181937 None:intergenic 55.0%
!! CACTCTTAAAGAGTGCCCCG+GGG - tig0019015:182027-182046 Msa1370080:intron 55.0%
!! ACTCTTAAAGAGTGCCCCGG+GGG - tig0019015:182028-182047 Msa1370080:intron 55.0%
TCTTGTTAACGAGTGCCCCC+GGG + tig0019015:182046-182065 None:intergenic 55.0%
! GTCTTGTTAACGAGTGCCCC+CGG + tig0019015:182047-182066 None:intergenic 55.0%
GCACCAACAGCTTTCTCCAC+TGG + tig0019015:182355-182374 None:intergenic 55.0%
!! AGCTCTTAAGGAGTGCCCCG+GGG + tig0019015:181917-181936 None:intergenic 60.0%
CTTGTTAACGAGTGCCCCCG+GGG + tig0019015:182045-182064 None:intergenic 60.0%
!! AGCTCTTAAGGAGTGCCCCG+GGG + tig0019015:181917-181936 None:intergenic 60.0%
CTTGTTAACGAGTGCCCCCG+GGG + tig0019015:182045-182064 None:intergenic 60.0%
!! GCTCTTAAGGAGTGCCCCGG+GGG + tig0019015:181916-181935 None:intergenic 65.0%
!! GCTCTTAAGGAGTGCCCCGG+GGG + tig0019015:181916-181935 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0019015 gene 180390 182562 180390 ID=Msa1370080;Name=Msa1370080
tig0019015 mRNA 180390 182562 180390 ID=Msa1370080-mRNA-1;Parent=Msa1370080;Name=Msa1370080-mRNA-1;_AED=0.12;_eAED=0.12;_QI=330|1|1|1|1|1|3|118|126
tig0019015 exon 180390 180663 180390 ID=Msa1370080-mRNA-1:exon:1479;Parent=Msa1370080-mRNA-1
tig0019015 exon 181849 181971 181849 ID=Msa1370080-mRNA-1:exon:1478;Parent=Msa1370080-mRNA-1
tig0019015 exon 182131 182562 182131 ID=Msa1370080-mRNA-1:exon:1477;Parent=Msa1370080-mRNA-1
tig0019015 five_prime_UTR 182233 182562 182233 ID=Msa1370080-mRNA-1:five_prime_utr;Parent=Msa1370080-mRNA-1
tig0019015 CDS 182131 182232 182131 ID=Msa1370080-mRNA-1:cds;Parent=Msa1370080-mRNA-1
tig0019015 CDS 181849 181971 181849 ID=Msa1370080-mRNA-1:cds;Parent=Msa1370080-mRNA-1
tig0019015 CDS 180508 180663 180508 ID=Msa1370080-mRNA-1:cds;Parent=Msa1370080-mRNA-1
tig0019015 three_prime_UTR 180390 180507 180390 ID=Msa1370080-mRNA-1:three_prime_utr;Parent=Msa1370080-mRNA-1
Gene Sequence

>Msa1370080

ATGGGTAGTCAACAACAGCACAAAAAGTCCAAGGTTGTGAAAATTGACTCAAGAAAATCATGGGAACACCACATCACTGAAGCTACCAATAAAGGTTGCCTTGTTATGGTTCATTTTTCTGCTTATTGGTGCGTTCCTTCTATTGCTATGAACCCTTTCTTTGAAGAATTAGCATCCACCTATCAAGATGTCCTCTTTCTGAAGGTGGATGTGGACGAAGTTAAGGAAGTAGCCTCCAAGTTGGAAATCAATGCCATGCCTACCTTTTTGTTGATGAGTGGAGGAAATCCAGTGGAGAAAGCTGTTGGTGCAAATCCTGATGAAATTAGGAAAAGGATGGATCATTTTATTAACCAAACTCATTCCTCCAAATCACTCTGA
Protein sequence

>Msa1370080

MGSQQQHKKSKVVKIDSRKSWEHHITEATNKGCLVMVHFSAYWCVPSIAMNPFFEELASTYQDVLFLKVDVDEVKEVASKLEINAMPTFLLMSGGNPVEKAVGANPDEIRKRMDHFINQTHSSKSL*