Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0694610 | AFK38978.1 | 84.536 | 97 | 6 | 4 | 1 | 92 | 1 | 93 | 3.87e-42 | 146 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0694610 | sp|Q9XIM0|MEE14_ARATH | 63.636 | 66 | 21 | 1 | 27 | 92 | 35 | 97 | 1.23e-22 | 89.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0694610 | I3SFD6 | 84.536 | 97 | 6 | 4 | 1 | 92 | 1 | 93 | 1.85e-42 | 146 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0605650 | Msa0694610 | 0.819350 | 1.216226e-52 | -8.615850e-47 |
| Msa0611890 | Msa0694610 | 0.804634 | 1.966563e-49 | -8.615850e-47 |
| Msa0652050 | Msa0694610 | 0.905877 | 2.696762e-80 | -8.615850e-47 |
| Msa0694610 | Msa0867730 | 0.818043 | 2.407686e-52 | -8.615850e-47 |
| Msa0156370 | Msa0694610 | 0.810305 | 1.231267e-50 | -8.615850e-47 |
| Msa0556520 | Msa0694610 | 0.890012 | 1.446321e-73 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0694610 | MtrunA17_Chr4g0045491 | 84.536 | 97 | 6 | 4 | 1 | 92 | 1 | 93 | 3.97e-46 | 146 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0694610 | AT2G15890.2 | 63.636 | 66 | 21 | 1 | 27 | 92 | 35 | 97 | 9.32e-24 | 89.4 |
| Msa0694610 | AT2G15890.1 | 63.636 | 66 | 21 | 1 | 27 | 92 | 35 | 97 | 1.25e-23 | 89.7 |
Find 20 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAGTTTGGTTCGGCTAAA+AGG | 0.273045 | 4_4:+63939435 | None:intergenic |
| AATAATGGAAGAAGGAAGTT+TGG | 0.327631 | 4_4:+63939500 | None:intergenic |
| GGAAGTTTGGTTGAAGAAGT+TGG | 0.331092 | 4_4:+63939513 | None:intergenic |
| TCTAGAAGAACAACAAATAA+TGG | 0.365979 | 4_4:+63939485 | None:intergenic |
| AAAATCTGAGAAAGATCTCT+TGG | 0.387196 | 4_4:-63939382 | Msa0694610:CDS |
| CGACTCTGTCAAGTTTGGTT+CGG | 0.395191 | 4_4:+63939427 | None:intergenic |
| GAAGTTTGGTTGAAGAAGTT+GGG | 0.409888 | 4_4:+63939514 | None:intergenic |
| CGGCTAAAAGGTTCAAGCTT+AGG | 0.425055 | 4_4:+63939447 | None:intergenic |
| TTTAGCGACTCTGTCAAGTT+TGG | 0.428057 | 4_4:+63939422 | None:intergenic |
| GTGATGAATCATATAGTTGC+TGG | 0.434059 | 4_4:-63939259 | Msa0694610:CDS |
| TTGTTTGGTAGATTATTGTT+CGG | 0.474583 | 4_4:-63939291 | Msa0694610:intron |
| CAAGCTTAGGAATGAAAGGT+TGG | 0.486187 | 4_4:+63939460 | None:intergenic |
| GAAACAAATTTGGTCAAGAA+AGG | 0.487117 | 4_4:+63939080 | None:intergenic |
| AAATCTGAGAAAGATCTCTT+GGG | 0.506201 | 4_4:-63939381 | Msa0694610:intron |
| GGTTCAAGCTTAGGAATGAA+AGG | 0.526887 | 4_4:+63939456 | None:intergenic |
| GATTATTGTTCGGTACTTGA+AGG | 0.537788 | 4_4:-63939281 | Msa0694610:CDS |
| GAACAACAAATAATGGAAGA+AGG | 0.539572 | 4_4:+63939492 | None:intergenic |
| TTGCGATGAAGAAACAGAGA+AGG | 0.583672 | 4_4:+63939537 | None:intergenic |
| TGAATCATATAGTTGCTGGC+AGG | 0.600681 | 4_4:-63939255 | Msa0694610:CDS |
| TGCGATGAAGAAACAGAGAA+GGG | 0.638715 | 4_4:+63939538 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTATGTTATTTGATTT+TGG | - | chr4_4:63939331-63939350 | Msa0694610:intron | 10.0% |
| !! | AAAATTAAATGAAAAACAAC+AGG | + | chr4_4:63939483-63939502 | None:intergenic | 15.0% |
| !!! | TTTTGGTTTATGATTTTGTT+TGG | - | chr4_4:63939348-63939367 | Msa0694610:intron | 20.0% |
| ! | TCTAGAAGAACAACAAATAA+TGG | + | chr4_4:63939172-63939191 | None:intergenic | 25.0% |
| !!! | TTCTCAGATTTTTCAATCAA+AGG | + | chr4_4:63939264-63939283 | None:intergenic | 25.0% |
| ! | ACACGAAATAAGAAAGAAAA+CGG | + | chr4_4:63939305-63939324 | None:intergenic | 25.0% |
| ! | TTGTTTGGTAGATTATTGTT+CGG | - | chr4_4:63939363-63939382 | Msa0694610:intron | 25.0% |
| ! | AATAATGGAAGAAGGAAGTT+TGG | + | chr4_4:63939157-63939176 | None:intergenic | 30.0% |
| ! | GAACAACAAATAATGGAAGA+AGG | + | chr4_4:63939165-63939184 | None:intergenic | 30.0% |
| !! | CAGATTTTTCAATCAAAGGA+AGG | + | chr4_4:63939260-63939279 | None:intergenic | 30.0% |
| AAAATCTGAGAAAGATCTCT+TGG | - | chr4_4:63939272-63939291 | Msa0694610:CDS | 30.0% | |
| AAATCTGAGAAAGATCTCTT+GGG | - | chr4_4:63939273-63939292 | Msa0694610:CDS | 30.0% | |
| ! | TCTTCTTTGTTTGAGAGATT+TGG | - | chr4_4:63939502-63939521 | Msa0694610:CDS | 30.0% |
| ACACAGAAACAGAAATATCA+GGG | + | chr4_4:63939534-63939553 | None:intergenic | 30.0% | |
| GAAGTTTGGTTGAAGAAGTT+GGG | + | chr4_4:63939143-63939162 | None:intergenic | 35.0% | |
| GATTATTGTTCGGTACTTGA+AGG | - | chr4_4:63939373-63939392 | Msa0694610:intron | 35.0% | |
| GTGATGAATCATATAGTTGC+TGG | - | chr4_4:63939395-63939414 | Msa0694610:CDS | 35.0% | |
| CACACAGAAACAGAAATATC+AGG | + | chr4_4:63939535-63939554 | None:intergenic | 35.0% | |
| TGCGATGAAGAAACAGAGAA+GGG | + | chr4_4:63939119-63939138 | None:intergenic | 40.0% | |
| TTGCGATGAAGAAACAGAGA+AGG | + | chr4_4:63939120-63939139 | None:intergenic | 40.0% | |
| GGAAGTTTGGTTGAAGAAGT+TGG | + | chr4_4:63939144-63939163 | None:intergenic | 40.0% | |
| CAAGCTTAGGAATGAAAGGT+TGG | + | chr4_4:63939197-63939216 | None:intergenic | 40.0% | |
| GGTTCAAGCTTAGGAATGAA+AGG | + | chr4_4:63939201-63939220 | None:intergenic | 40.0% | |
| !! | TCAAGTTTGGTTCGGCTAAA+AGG | + | chr4_4:63939222-63939241 | None:intergenic | 40.0% |
| TTTAGCGACTCTGTCAAGTT+TGG | + | chr4_4:63939235-63939254 | None:intergenic | 40.0% | |
| TGAATCATATAGTTGCTGGC+AGG | - | chr4_4:63939399-63939418 | Msa0694610:CDS | 40.0% | |
| CGGCTAAAAGGTTCAAGCTT+AGG | + | chr4_4:63939210-63939229 | None:intergenic | 45.0% | |
| CGACTCTGTCAAGTTTGGTT+CGG | + | chr4_4:63939230-63939249 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 63939093 | 63939583 | 63939093 | ID=Msa0694610;Name=Msa0694610 |
| chr4_4 | mRNA | 63939093 | 63939583 | 63939093 | ID=Msa0694610-mRNA-1;Parent=Msa0694610;Name=Msa0694610-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0.5|0.33|0.66|1|1|3|0|97 |
| chr4_4 | exon | 63939382 | 63939583 | 63939382 | ID=Msa0694610-mRNA-1:exon:18196;Parent=Msa0694610-mRNA-1 |
| chr4_4 | exon | 63939229 | 63939302 | 63939229 | ID=Msa0694610-mRNA-1:exon:18195;Parent=Msa0694610-mRNA-1 |
| chr4_4 | exon | 63939093 | 63939110 | 63939093 | ID=Msa0694610-mRNA-1:exon:18194;Parent=Msa0694610-mRNA-1 |
| chr4_4 | CDS | 63939382 | 63939583 | 63939382 | ID=Msa0694610-mRNA-1:cds;Parent=Msa0694610-mRNA-1 |
| chr4_4 | CDS | 63939229 | 63939302 | 63939229 | ID=Msa0694610-mRNA-1:cds;Parent=Msa0694610-mRNA-1 |
| chr4_4 | CDS | 63939093 | 63939110 | 63939093 | ID=Msa0694610-mRNA-1:cds;Parent=Msa0694610-mRNA-1 |
| Gene Sequence |
| Protein sequence |
