AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605650 MtrunA17_Chr4g0035051 98.824 85 1 0 1 85 1 85 1.06e-58 178
Msa0605650 MtrunA17_Chr4g0035001 90.244 82 8 0 4 85 2 83 1.32e-51 162
Msa0605650 MtrunA17_Chr4g0035031 86.047 86 12 0 1 86 1 86 2.67e-50 160
Msa0605650 MtrunA17_Chr2g0278661 55.056 89 39 1 1 89 1 88 5.80e-32 114
Msa0605650 MtrunA17_Chr4g0050781 52.874 87 40 1 1 87 1 86 3.04e-29 106
Msa0605650 MtrunA17_Chr7g0219021 46.154 91 45 1 1 91 37 123 2.61e-23 92.4
Msa0605650 MtrunA17_Chr4g0061451 48.276 87 44 1 1 87 36 121 3.19e-23 92.4
Msa0605650 MtrunA17_Chr1g0210241 50.617 81 40 0 7 87 6 86 3.97e-23 92.4
Msa0605650 MtrunA17_Chr3g0116631 48.148 81 42 0 8 88 206 286 4.36e-23 92.4
Msa0605650 MtrunA17_Chr3g0116631 31.206 141 92 2 9 144 110 250 8.37e-13 64.7
Msa0605650 MtrunA17_Chr3g0086861 44.706 85 47 0 3 87 39 123 7.30e-22 90.9
Msa0605650 MtrunA17_Chr4g0058151 46.914 81 43 0 8 88 216 296 3.01e-20 85.1
Msa0605650 MtrunA17_Chr4g0058151 36.538 104 64 1 7 110 121 222 8.62e-16 72.8
Msa0605650 MtrunA17_Chr1g0181111 48.750 80 41 0 9 88 197 276 1.21e-19 83.2
Msa0605650 MtrunA17_Chr3g0121631 48.544 103 52 1 9 111 37 138 7.09e-19 78.2
Msa0605650 MtrunA17_Chr4g0017401 40.171 117 58 4 5 111 12 126 4.43e-18 78.6
Msa0605650 MtrunA17_Chr7g0266751 37.500 96 57 1 6 98 30 125 5.60e-18 75.9
Msa0605650 MtrunA17_Chr3g0119311 39.024 82 50 0 9 90 37 118 7.22e-18 75.5
Msa0605650 MtrunA17_Chr1g0168601 40.244 82 49 0 4 85 149 230 1.90e-17 76.6
Msa0605650 MtrunA17_Chr3g0144311 42.222 90 48 2 9 95 30 118 3.75e-17 76.3
Msa0605650 MtrunA17_Chr3g0101061 38.144 97 58 1 9 105 41 135 1.40e-16 76.3
Msa0605650 MtrunA17_Chr3g0127751 40.230 87 51 1 9 95 27 112 1.92e-16 73.9
Msa0605650 MtrunA17_Chr6g0481071 37.931 87 54 0 9 95 96 182 4.37e-16 72.0
Msa0605650 MtrunA17_Chr3g0088511 44.595 74 41 0 9 82 33 106 6.34e-16 70.1
Msa0605650 MtrunA17_Chr5g0426311 44.737 76 41 1 9 84 17 91 7.50e-16 72.8
Msa0605650 MtrunA17_Chr8g0346011 33.582 134 73 4 9 128 38 169 1.45e-15 72.4
Msa0605650 MtrunA17_Chr7g0276531 50.847 59 29 0 9 67 19 77 3.02e-15 70.9
Msa0605650 MtrunA17_Chr4g0018581 34.884 129 80 2 10 137 453 578 3.43e-15 72.0
Msa0605650 MtrunA17_Chr1g0186351 35.443 79 49 1 9 87 7 83 4.68e-15 71.6
Msa0605650 MtrunA17_Chr1g0186351 27.097 155 98 2 9 149 113 266 1.35e-12 64.7
Msa0605650 MtrunA17_Chr7g0263001 40.506 79 47 0 9 87 36 114 4.92e-15 68.2
Msa0605650 MtrunA17_Chr5g0447761 36.082 97 60 1 9 105 7 101 6.12e-15 71.2
Msa0605650 MtrunA17_Chr5g0447761 32.184 87 58 1 9 95 107 192 4.54e-11 60.1
Msa0605650 MtrunA17_Chr2g0290191 36.709 79 48 1 9 87 5 81 2.65e-14 69.3
Msa0605650 MtrunA17_Chr3g0120721 32.847 137 83 2 9 145 107 234 3.17e-14 68.9
Msa0605650 MtrunA17_Chr3g0120721 42.857 77 35 1 9 85 19 86 3.41e-13 66.2
Msa0605650 MtrunA17_Chr7g0219031 40.909 66 39 0 9 74 41 106 6.36e-14 64.7
Msa0605650 MtrunA17_Chr2g0327921 35.366 82 53 0 9 90 8 89 2.65e-13 62.0
Msa0605650 MtrunA17_Chr6g0449481 38.554 83 49 1 9 91 46 126 2.82e-13 66.2
Msa0605650 MtrunA17_Chr4g0042161 39.286 84 45 2 11 91 54 134 3.94e-13 63.2
Msa0605650 MtrunA17_Chr4g0062931 34.132 167 84 6 10 164 9 161 6.87e-13 65.5
Msa0605650 MtrunA17_Chr7g0267571 36.667 90 57 0 4 93 208 297 9.95e-13 64.7
Msa0605650 MtrunA17_Chr4g0065741 34.783 92 59 1 9 100 72 162 1.63e-12 62.0
Msa0605650 MtrunA17_Chr4g0031091 38.356 73 45 0 9 81 34 106 4.04e-12 59.7
Msa0605650 MtrunA17_Chr3g0135111 35.714 98 57 2 9 102 11 106 4.35e-12 62.8
Msa0605650 MtrunA17_Chr5g0402191 41.250 80 43 2 9 87 5 81 9.61e-12 62.0
Msa0605650 MtrunA17_Chr3g0110211 38.889 90 54 1 3 91 107 196 3.24e-11 60.5
Msa0605650 MtrunA17_Chr5g0419351 38.554 83 51 0 10 92 12 94 4.15e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605650 AT2G21660.2 84.706 85 13 0 1 85 1 85 8.18e-49 155
Msa0605650 AT2G21660.1 84.706 85 13 0 1 85 1 85 1.38e-48 155
Msa0605650 AT4G39260.3 78.889 90 19 0 4 93 2 91 1.79e-48 152
Msa0605650 AT4G39260.2 76.829 82 19 0 4 85 2 83 2.14e-43 140
Msa0605650 AT4G39260.1 76.829 82 19 0 4 85 2 83 7.85e-43 140
Msa0605650 AT3G26420.1 51.429 105 49 1 3 107 2 104 2.66e-31 113
Msa0605650 AT4G39260.4 59.259 108 18 1 4 111 2 83 6.74e-28 100
Msa0605650 AT3G23830.2 50.602 83 41 0 9 91 36 118 1.83e-24 92.8
Msa0605650 AT3G23830.1 50.602 83 41 0 9 91 36 118 1.83e-24 92.8
Msa0605650 AT4G13850.4 51.852 81 39 0 9 89 36 116 2.34e-24 92.4
Msa0605650 AT4G13850.2 50.602 83 41 0 9 91 36 118 2.52e-24 92.8
Msa0605650 AT5G61030.2 48.101 79 41 0 9 87 41 119 2.75e-23 93.6
Msa0605650 AT5G61030.1 48.101 79 41 0 9 87 41 119 2.75e-23 93.6
Msa0605650 AT4G13850.3 51.064 94 46 0 9 102 36 129 3.77e-23 89.7
Msa0605650 AT4G13850.1 51.064 94 46 0 9 102 36 129 5.38e-23 89.7
Msa0605650 AT5G04280.1 46.465 99 52 1 9 107 8 105 1.90e-21 89.0
Msa0605650 AT5G50250.1 45.783 83 45 0 5 87 204 286 2.44e-21 88.2
Msa0605650 AT5G50250.1 34.483 145 83 3 7 144 112 251 5.40e-16 73.9
Msa0605650 AT2G21690.1 50.000 90 40 1 3 87 2 91 2.49e-21 84.0
Msa0605650 AT3G53460.1 44.578 83 46 0 9 91 258 340 3.73e-21 88.6
Msa0605650 AT3G53460.3 44.578 83 46 0 9 91 258 340 3.73e-21 88.6
Msa0605650 AT3G53460.2 44.578 83 46 0 9 91 258 340 3.73e-21 88.6
Msa0605650 AT2G37220.1 44.444 81 45 0 9 89 205 285 4.36e-21 87.4
Msa0605650 AT2G37220.1 41.250 80 45 1 3 82 88 165 2.48e-11 60.8
Msa0605650 AT3G53460.4 44.578 83 46 0 9 91 279 361 4.94e-21 88.2
Msa0605650 AT4G24770.1 43.902 82 46 0 8 89 244 325 5.04e-21 87.8
Msa0605650 AT4G24770.1 37.815 119 65 2 7 118 149 265 5.58e-16 73.9
Msa0605650 AT4G24770.2 43.902 82 46 0 8 89 244 325 5.04e-21 87.8
Msa0605650 AT4G24770.2 37.815 119 65 2 7 118 149 265 5.58e-16 73.9
Msa0605650 AT1G74230.1 52.632 76 35 1 9 84 35 109 3.79e-20 85.1
Msa0605650 AT1G60650.2 38.095 126 73 2 5 125 9 134 7.03e-20 84.3
Msa0605650 AT1G60650.1 38.095 126 73 2 5 125 9 134 7.03e-20 84.3
Msa0605650 AT5G06210.1 41.573 89 52 0 6 94 32 120 3.19e-19 79.3
Msa0605650 AT1G76460.1 48.684 76 38 1 9 84 25 99 4.11e-18 79.3
Msa0605650 AT1G20880.2 47.368 76 39 1 9 84 25 99 5.68e-18 79.0
Msa0605650 AT1G20880.3 47.368 76 39 1 9 84 25 99 5.68e-18 79.0
Msa0605650 AT1G20880.1 47.368 76 39 1 9 84 25 99 5.68e-18 79.0
Msa0605650 AT1G18630.1 44.872 78 43 0 9 86 37 114 1.59e-17 75.5
Msa0605650 AT1G22330.1 42.308 104 58 2 9 112 18 119 2.33e-17 77.4
Msa0605650 AT5G55550.2 35.780 109 68 1 9 117 7 113 3.25e-17 78.2
Msa0605650 AT5G55550.3 35.780 109 68 1 9 117 7 113 3.25e-17 78.2
Msa0605650 AT5G55550.6 35.780 109 68 1 9 117 7 113 3.25e-17 78.2
Msa0605650 AT5G55550.11 35.780 109 68 1 9 117 7 113 3.25e-17 78.2
Msa0605650 AT5G55550.5 35.780 109 68 1 9 117 7 113 3.44e-17 78.2
Msa0605650 AT5G55550.4 35.780 109 68 1 9 117 7 113 3.44e-17 78.2
Msa0605650 AT5G55550.9 35.780 109 68 1 9 117 7 113 3.44e-17 78.2
Msa0605650 AT5G55550.7 35.780 109 68 1 9 117 7 113 3.44e-17 78.2
Msa0605650 AT5G55550.1 35.780 109 68 1 9 117 7 113 3.44e-17 78.2
Msa0605650 AT1G22910.1 40.404 99 54 3 5 99 9 106 8.96e-17 75.5
Msa0605650 AT1G22910.2 40.404 99 54 3 5 99 9 106 1.13e-16 75.1
Msa0605650 AT2G46780.1 43.421 76 42 1 9 84 23 97 1.68e-16 75.5
Msa0605650 AT2G46780.2 43.421 76 42 1 9 84 23 97 1.68e-16 75.5
Msa0605650 AT3G07810.2 41.772 79 44 1 9 87 7 83 1.71e-16 76.3
Msa0605650 AT3G07810.1 41.772 79 44 1 9 87 7 83 1.74e-16 76.3
Msa0605650 AT4G26650.2 34.951 103 65 1 9 111 13 113 3.52e-16 75.1
Msa0605650 AT4G26650.1 34.951 103 65 1 9 111 16 116 3.57e-16 75.1
Msa0605650 AT1G22910.3 40.404 99 54 3 5 99 9 106 6.83e-16 73.9
Msa0605650 AT4G14300.1 36.667 90 55 1 9 98 7 94 1.47e-15 73.6
Msa0605650 AT4G14300.2 36.667 90 55 1 9 98 7 94 1.47e-15 73.6
Msa0605650 AT2G33410.1 34.831 89 56 1 9 97 7 93 1.67e-15 73.2
Msa0605650 AT2G33410.1 36.709 79 49 1 9 87 111 188 3.36e-11 60.8
Msa0605650 AT1G78260.1 43.210 81 44 2 5 84 13 92 2.30e-15 72.0
Msa0605650 AT1G60000.1 40.244 82 49 0 4 85 173 254 2.41e-15 71.6
Msa0605650 AT4G13860.1 41.892 74 43 0 9 82 4 77 2.48e-15 67.8
Msa0605650 AT1G33470.3 37.895 95 53 2 9 98 8 101 3.71e-15 70.9
Msa0605650 AT3G13224.3 37.234 94 53 2 6 95 4 95 4.06e-15 72.0
Msa0605650 AT3G13224.3 31.633 98 64 1 9 106 101 195 3.95e-13 66.2
Msa0605650 AT1G33470.2 37.895 95 53 2 9 98 8 101 4.28e-15 70.9
Msa0605650 AT1G33470.1 37.895 95 53 2 9 98 8 101 4.29e-15 70.9
Msa0605650 AT1G78260.2 43.210 81 44 2 5 84 13 92 4.37e-15 71.2
Msa0605650 AT3G13224.2 37.931 87 52 1 9 95 20 104 5.86e-15 71.6
Msa0605650 AT3G13224.2 32.955 88 58 1 3 89 103 190 2.00e-13 67.0
Msa0605650 AT5G19960.1 39.640 111 63 3 10 119 9 116 9.21e-15 70.9
Msa0605650 AT5G47320.1 41.558 77 45 0 9 85 32 108 9.26e-15 69.3
Msa0605650 AT5G59865.1 43.038 79 45 0 11 89 29 107 9.31e-15 67.0
Msa0605650 AT3G08000.1 41.667 84 49 0 9 92 42 125 1.27e-14 67.4
Msa0605650 AT3G54770.3 44.737 76 41 1 9 84 18 92 1.43e-14 69.7
Msa0605650 AT3G54770.1 44.737 76 41 1 9 84 18 92 1.52e-14 69.3
Msa0605650 AT5G55550.8 43.662 71 38 1 9 79 7 75 2.74e-14 69.7
Msa0605650 AT2G14160.1 40.698 86 32 2 1 68 1 85 3.00e-14 65.1
Msa0605650 AT2G37510.1 43.243 74 42 0 9 82 35 108 3.09e-14 66.2
Msa0605650 AT5G55550.10 43.662 71 38 1 9 79 7 75 3.38e-14 69.7
Msa0605650 AT3G46020.1 38.462 78 48 0 9 86 8 85 1.22e-13 63.9
Msa0605650 AT1G17640.2 40.000 80 46 1 9 88 67 144 1.31e-13 67.8
Msa0605650 AT1G17640.2 35.366 82 53 0 9 90 158 239 2.69e-11 61.2
Msa0605650 AT1G17640.1 40.000 80 46 1 9 88 67 144 1.31e-13 67.8
Msa0605650 AT1G17640.1 35.366 82 53 0 9 90 158 239 2.69e-11 61.2
Msa0605650 AT1G17640.3 40.000 80 46 1 9 88 67 144 1.31e-13 67.8
Msa0605650 AT1G17640.3 35.366 82 53 0 9 90 158 239 2.69e-11 61.2
Msa0605650 AT3G13224.1 37.931 87 52 1 9 95 20 104 1.73e-13 66.2
Msa0605650 AT5G53720.1 41.250 80 46 1 5 84 6 84 1.74e-13 63.2
Msa0605650 AT3G52150.2 39.759 83 50 0 2 84 171 253 2.67e-13 65.9
Msa0605650 AT3G52150.1 39.759 83 50 0 2 84 171 253 2.67e-13 65.9
Msa0605650 AT1G01080.1 37.975 79 44 2 7 83 211 286 3.28e-13 66.2
Msa0605650 AT1G01080.2 37.975 79 44 2 7 83 212 287 3.40e-13 66.2
Msa0605650 AT5G51300.1 30.400 125 86 1 7 131 479 602 3.92e-13 66.6
Msa0605650 AT5G51300.2 30.400 125 86 1 7 131 479 602 3.92e-13 66.6
Msa0605650 AT5G51300.3 30.400 125 86 1 7 131 479 602 3.92e-13 66.6
Msa0605650 AT2G27330.1 26.667 90 66 0 10 99 23 112 9.31e-13 62.0
Msa0605650 AT1G73530.1 35.000 80 52 0 9 88 78 157 9.73e-13 63.2
Msa0605650 AT5G47620.1 35.443 79 49 1 9 87 7 83 9.97e-13 65.5
Msa0605650 AT5G47620.2 35.443 79 49 1 9 87 7 83 9.97e-13 65.5
Msa0605650 AT5G47620.5 35.443 79 49 1 9 87 7 83 9.97e-13 65.5
Msa0605650 AT5G40490.1 43.210 81 44 1 9 89 43 121 1.13e-12 65.1
Msa0605650 AT5G53680.1 44.928 69 37 1 9 77 14 81 1.16e-12 62.8
Msa0605650 AT5G54580.1 43.590 78 44 0 10 87 58 135 2.91e-12 61.6
Msa0605650 AT5G11412.1 43.373 83 45 2 3 84 46 127 5.30e-12 61.2
Msa0605650 AT5G47620.4 47.059 51 27 0 9 59 7 57 1.20e-11 62.4
Msa0605650 AT4G13860.2 44.068 59 33 0 24 82 2 60 1.51e-11 57.4
Msa0605650 AT3G06970.1 26.016 123 87 2 9 128 13 134 1.62e-11 61.6
Msa0605650 AT3G47120.2 38.667 75 46 0 10 84 38 112 2.01e-11 61.2
Msa0605650 AT3G47120.1 39.189 74 45 0 11 84 39 112 2.04e-11 61.2
Msa0605650 AT1G58470.2 40.541 74 39 2 8 77 6 78 2.97e-11 60.8
Msa0605650 AT1G58470.1 40.541 74 39 2 8 77 6 78 2.97e-11 60.8
Msa0605650 AT5G64200.2 37.179 78 49 0 8 85 16 93 3.98e-11 60.5
Msa0605650 AT5G64200.1 37.179 78 49 0 8 85 16 93 3.98e-11 60.5
Msa0605650 AT3G52380.1 35.714 84 54 0 4 87 215 298 4.69e-11 60.5
Msa0605650 AT3G52380.1 37.838 74 46 0 9 82 117 190 4.83e-11 60.1
Msa0605650 AT3G56860.9 41.270 63 37 0 5 67 137 199 8.51e-11 59.7
Msa0605650 AT3G56860.11 41.270 63 37 0 5 67 137 199 8.51e-11 59.7
Msa0605650 AT3G56860.8 41.270 63 37 0 5 67 137 199 8.51e-11 59.7
Msa0605650 AT3G56860.5 41.270 63 37 0 5 67 137 199 8.51e-11 59.7
Msa0605650 AT3G56860.7 41.270 63 37 0 5 67 137 199 8.51e-11 59.7
Msa0605650 AT3G56860.2 41.270 63 37 0 5 67 137 199 8.51e-11 59.7
Msa0605650 AT3G56860.6 41.270 63 37 0 5 67 137 199 8.51e-11 59.7
Msa0605650 AT3G56860.10 41.270 63 37 0 5 67 137 199 8.51e-11 59.7
Msa0605650 AT3G56860.3 41.270 63 37 0 5 67 137 199 8.51e-11 59.7
Msa0605650 AT3G56860.1 41.270 63 37 0 5 67 137 199 8.51e-11 59.7
Msa0605650 AT3G56860.4 41.270 63 37 0 5 67 137 199 8.51e-11 59.7

Find 74 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGAGGTGGTGGTGGTTA+TGG 0.262639 4_2:+51597840 Msa0605650:CDS
GGTGGAGGTGGTGGTGGATA+TGG 0.277157 4_2:+51597756 Msa0605650:CDS
GGCCGTGGTGGCGGTGGATA+TGG 0.293898 4_2:+51597735 Msa0605650:CDS
ACTGGAAGATCAAGAGGATT+TGG 0.294131 4_2:+51597588 Msa0605650:CDS
GGTGGTTATGGAGGAGGAGG+TGG 0.328302 4_2:+51597828 Msa0605650:CDS
GGAGGAGGCCGTGGTGGCGG+TGG 0.329157 4_2:+51597729 Msa0605650:CDS
AGTGGTGGTGGAGGTGGTTA+TGG 0.334178 4_2:+51597816 Msa0605650:CDS
GGTTATGGAGGAGGAGGTGG+TGG 0.336090 4_2:+51597831 Msa0605650:CDS
GGAGGTGGTGGTGGTTATGG+TGG 0.347344 4_2:+51597843 Msa0605650:CDS
GGTGGATATGGTGGAGGTGG+TGG 0.349052 4_2:+51597747 Msa0605650:CDS
ATTATCAACGATCGTGAAAC+TGG 0.354445 4_2:+51597570 Msa0605650:CDS
GGTGGTAGGGACCGTGGTTA+TGG 0.354584 4_2:+51597864 Msa0605650:CDS
GTGGTGGTTATGGTGGTGGT+AGG 0.359680 4_2:+51597850 Msa0605650:CDS
GGAGGTGGTGGTGGATATGG+CGG 0.376984 4_2:+51597759 Msa0605650:CDS
GGTGGAGGAGGCCGTGGTGG+CGG 0.379339 4_2:+51597726 Msa0605650:CDS
GGATATGGTGGAGGTGGTGG+TGG 0.393513 4_2:+51597750 Msa0605650:CDS
TATGGAGGAGGAGGTGGTGG+TGG 0.400299 4_2:+51597834 Msa0605650:CDS
AAGCTCAGAGCCGTGGTAGC+GGG 0.414264 4_2:+51597703 Msa0605650:CDS
GGCGGTGGATATGGTGGAGG+TGG 0.414719 4_2:+51597744 Msa0605650:CDS
GGTGGAGGTGGTTATGGAGG+AGG 0.415959 4_2:+51597822 Msa0605650:CDS
GTGGAGATGGTGGAGGAAGC+TGG 0.423465 4_2:+51597925 Msa0605650:CDS
GGAGGTGGTTATGGAGGAGG+AGG 0.430587 4_2:+51597825 Msa0605650:CDS
AGGGATGAACGGTTCAGATA+TGG 0.435664 4_2:+51597656 Msa0605650:CDS
GCTTCGTCGGAGGTCTTGCA+TGG 0.446995 4_2:+51597168 Msa0605650:CDS
GGTGGTGGTGGATATGGCGG+AGG 0.453193 4_2:+51597762 Msa0605650:CDS
GGTGGTGGATATGGCGGAGG+TGG 0.460996 4_2:+51597765 Msa0605650:CDS
GGTGGTGGAGGTGGTTATGG+AGG 0.464167 4_2:+51597819 Msa0605650:CDS
TATTCTTCACGCGGTGGAGA+TGG 0.477424 4_2:+51597912 Msa0605650:CDS
TGGTGGTTATGGTGGTGGTA+GGG 0.478980 4_2:+51597851 Msa0605650:CDS
GGTGGTGGTGGTTATGGTGG+TGG 0.479742 4_2:+51597846 Msa0605650:CDS
GGTTATGGTGGTGGAGATGA+TGG 0.485104 4_2:+51597879 Msa0605650:CDS
GTTGAGTACCGTTGCTTCGT+CGG 0.485717 4_2:+51597155 Msa0605650:CDS
AGAAGCAGTGGTGGTGGAGG+TGG 0.500860 4_2:+51597810 Msa0605650:CDS
TACAGCAGAAGCAGTGGTGG+TGG 0.506540 4_2:+51597804 Msa0605650:CDS
GGCGGAGGTGGTCGCCGCGA+GGG 0.508855 4_2:+51597777 Msa0605650:CDS
AGCTCAGAGCCGTGGTAGCG+GGG 0.514633 4_2:+51597704 Msa0605650:CDS
AGCAGAAGCAGTGGTGGTGG+AGG 0.523650 4_2:+51597807 Msa0605650:CDS
TCGATGAGAGATGCTATTGA+AGG 0.523656 4_2:+51597636 Msa0605650:CDS
AGGGACCGTGGTTATGGTGG+TGG 0.524349 4_2:+51597870 Msa0605650:CDS
GAAGCTCAGAGCCGTGGTAG+CGG 0.526058 4_2:+51597702 Msa0605650:CDS
GAGAAAGCCTTCTCTCAATA+CGG 0.531059 4_2:+51597212 Msa0605650:CDS
TCTTCACGCGGTGGAGATGG+TGG 0.540242 4_2:+51597915 Msa0605650:CDS
CTTCGTCGGAGGTCTTGCAT+GGG 0.553297 4_2:+51597169 Msa0605650:CDS
CGTGGTGGCGGTGGATATGG+TGG 0.562612 4_2:+51597738 Msa0605650:CDS
TCAACATCTGCAGAAGCCAT+TGG 0.563245 4_2:-51597137 None:intergenic
GGAGGTGGTCGCCGCGAGGG+TGG 0.563434 4_2:+51597780 Msa0605650:CDS
CGGAGAAATCATCGATTCGA+AGG 0.564623 4_2:+51597232 Msa0605650:CDS
CTCGAGAGCTTCGCTATCGG+TGG 0.564705 4_2:-51597192 None:intergenic
GGTAGGGACCGTGGTTATGG+TGG 0.569427 4_2:+51597867 Msa0605650:CDS
GATTTCTCCGTATTGAGAGA+AGG 0.569467 4_2:-51597219 None:intergenic
CACCATATCCACCGCCACCA+CGG 0.571564 4_2:-51597737 None:intergenic
TCCACCGCGTGAAGAATAGC+GGG 0.572176 4_2:-51597907 None:intergenic
CGTGAAACTGGAAGATCAAG+AGG 0.575579 4_2:+51597582 Msa0605650:CDS
TATGGTGGTGGAGATGATGG+TGG 0.576911 4_2:+51597882 Msa0605650:CDS
GGTGGCGGTGGATATGGTGG+AGG 0.578322 4_2:+51597741 Msa0605650:CDS
TTTCTCGAGAGCTTCGCTAT+CGG 0.583736 4_2:-51597195 None:intergenic
CGATGAGAGATGCTATTGAA+GGG 0.586298 4_2:+51597637 Msa0605650:CDS
GCTCAGAGCCGTGGTAGCGG+GGG 0.586564 4_2:+51597705 Msa0605650:CDS
CAAGACCTCCGACGAAGCAA+CGG 0.604652 4_2:-51597163 None:intergenic
TCCCGCTATTCTTCACGCGG+TGG 0.608702 4_2:+51597906 Msa0605650:CDS
TCACGCGGTGGAGATGGTGG+AGG 0.610396 4_2:+51597918 Msa0605650:CDS
CTCCACCGCGTGAAGAATAG+CGG 0.613449 4_2:-51597908 None:intergenic
GAGTACCGTTGCTTCGTCGG+AGG 0.615538 4_2:+51597158 Msa0605650:CDS
GGTTACAGCAGAAGCAGTGG+TGG 0.617302 4_2:+51597801 Msa0605650:CDS
TGGCGGAGGTGGTCGCCGCG+AGG 0.618580 4_2:+51597776 Msa0605650:CDS
GGTGGTTACAGCAGAAGCAG+TGG 0.635095 4_2:+51597798 Msa0605650:CDS
TATGGTGGTGGTAGGGACCG+TGG 0.636326 4_2:+51597858 Msa0605650:CDS
ACGGTTCAGATATGGATGGA+AGG 0.652700 4_2:+51597664 Msa0605650:CDS
GATGCTATTGAAGGGATGAA+CGG 0.654041 4_2:+51597645 Msa0605650:CDS
ATGAACGGTTCAGATATGGA+TGG 0.654412 4_2:+51597660 Msa0605650:CDS
GTGAACGAAGCTCAGAGCCG+TGG 0.656027 4_2:+51597696 Msa0605650:CDS
GGTTCCCGCTATTCTTCACG+CGG 0.677830 4_2:+51597903 Msa0605650:CDS
CATCTCCACCACCATAACCA+CGG 0.721401 4_2:-51597875 None:intergenic
TTCTGCTGTAACCACCCTCG+CGG 0.741441 4_2:-51597791 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTAGATCTGATTTTG+TGG + chr4_2:51597530-51597549 Msa0605650:intron 20.0%
!!! AATTTTTAGATCTGATTTTG+TGG + chr4_2:51597530-51597549 Msa0605650:intron 20.0%
!!! TGTTTTAGATTTTTCACCAA+TGG + chr4_2:51597121-51597140 Msa0605650:exon 25.0%
!!! TGTTTTAGATTTTTCACCAA+TGG + chr4_2:51597121-51597140 Msa0605650:exon 25.0%
!!! GATCTGACACGATTTTGATT+CGG + chr4_2:51597286-51597305 Msa0605650:intron 35.0%
AGATAGATCACAGATCAGAA+AGG - chr4_2:51597325-51597344 None:intergenic 35.0%
! ATTTTGTTACTCACCCCAAT+AGG + chr4_2:51597428-51597447 Msa0605650:intron 35.0%
TGAAGATGAACGTACCTATT+GGG - chr4_2:51597445-51597464 None:intergenic 35.0%
ATGAAGATGAACGTACCTAT+TGG - chr4_2:51597446-51597465 None:intergenic 35.0%
ACCAAAAGAAGAAGATCGAT+CGG + chr4_2:51597472-51597491 Msa0605650:intron 35.0%
AAGATCGATCGGTTACTATA+TGG + chr4_2:51597483-51597502 Msa0605650:intron 35.0%
ATTATCAACGATCGTGAAAC+TGG + chr4_2:51597570-51597589 Msa0605650:CDS 35.0%
!!! GATCTGACACGATTTTGATT+CGG + chr4_2:51597286-51597305 Msa0605650:intron 35.0%
AGATAGATCACAGATCAGAA+AGG - chr4_2:51597325-51597344 None:intergenic 35.0%
! ATTTTGTTACTCACCCCAAT+AGG + chr4_2:51597428-51597447 Msa0605650:intron 35.0%
TGAAGATGAACGTACCTATT+GGG - chr4_2:51597445-51597464 None:intergenic 35.0%
ATGAAGATGAACGTACCTAT+TGG - chr4_2:51597446-51597465 None:intergenic 35.0%
ACCAAAAGAAGAAGATCGAT+CGG + chr4_2:51597472-51597491 Msa0605650:intron 35.0%
AAGATCGATCGGTTACTATA+TGG + chr4_2:51597483-51597502 Msa0605650:intron 35.0%
ATTATCAACGATCGTGAAAC+TGG + chr4_2:51597570-51597589 Msa0605650:CDS 35.0%
GAGAAAGCCTTCTCTCAATA+CGG + chr4_2:51597212-51597231 Msa0605650:CDS 40.0%
GATTTCTCCGTATTGAGAGA+AGG - chr4_2:51597222-51597241 None:intergenic 40.0%
CAGAGTAACACAGAACAGAT+CGG - chr4_2:51597348-51597367 None:intergenic 40.0%
GAAGATGAACGTACCTATTG+GGG - chr4_2:51597444-51597463 None:intergenic 40.0%
! ACCGATCGATCTTCTTCTTT+TGG - chr4_2:51597476-51597495 None:intergenic 40.0%
ACTGGAAGATCAAGAGGATT+TGG + chr4_2:51597588-51597607 Msa0605650:CDS 40.0%
TCGATGAGAGATGCTATTGA+AGG + chr4_2:51597636-51597655 Msa0605650:CDS 40.0%
CGATGAGAGATGCTATTGAA+GGG + chr4_2:51597637-51597656 Msa0605650:CDS 40.0%
GATGCTATTGAAGGGATGAA+CGG + chr4_2:51597645-51597664 Msa0605650:CDS 40.0%
ATGAACGGTTCAGATATGGA+TGG + chr4_2:51597660-51597679 Msa0605650:CDS 40.0%
GAGAAAGCCTTCTCTCAATA+CGG + chr4_2:51597212-51597231 Msa0605650:CDS 40.0%
GATTTCTCCGTATTGAGAGA+AGG - chr4_2:51597222-51597241 None:intergenic 40.0%
CAGAGTAACACAGAACAGAT+CGG - chr4_2:51597348-51597367 None:intergenic 40.0%
GAAGATGAACGTACCTATTG+GGG - chr4_2:51597444-51597463 None:intergenic 40.0%
! ACCGATCGATCTTCTTCTTT+TGG - chr4_2:51597476-51597495 None:intergenic 40.0%
ACTGGAAGATCAAGAGGATT+TGG + chr4_2:51597588-51597607 Msa0605650:CDS 40.0%
TCGATGAGAGATGCTATTGA+AGG + chr4_2:51597636-51597655 Msa0605650:CDS 40.0%
CGATGAGAGATGCTATTGAA+GGG + chr4_2:51597637-51597656 Msa0605650:CDS 40.0%
GATGCTATTGAAGGGATGAA+CGG + chr4_2:51597645-51597664 Msa0605650:CDS 40.0%
ATGAACGGTTCAGATATGGA+TGG + chr4_2:51597660-51597679 Msa0605650:CDS 40.0%
TCAACATCTGCAGAAGCCAT+TGG - chr4_2:51597140-51597159 None:intergenic 45.0%
TTTCTCGAGAGCTTCGCTAT+CGG - chr4_2:51597198-51597217 None:intergenic 45.0%
!! CGGAGAAATCATCGATTCGA+AGG + chr4_2:51597232-51597251 Msa0605650:CDS 45.0%
!! TTTGTGTTGCTTGCAGGCTT+CGG + chr4_2:51597259-51597278 Msa0605650:intron 45.0%
CGTGAAACTGGAAGATCAAG+AGG + chr4_2:51597582-51597601 Msa0605650:CDS 45.0%
AGGGATGAACGGTTCAGATA+TGG + chr4_2:51597656-51597675 Msa0605650:CDS 45.0%
ACGGTTCAGATATGGATGGA+AGG + chr4_2:51597664-51597683 Msa0605650:CDS 45.0%
TCAACATCTGCAGAAGCCAT+TGG - chr4_2:51597140-51597159 None:intergenic 45.0%
TTTCTCGAGAGCTTCGCTAT+CGG - chr4_2:51597198-51597217 None:intergenic 45.0%
!! CGGAGAAATCATCGATTCGA+AGG + chr4_2:51597232-51597251 Msa0605650:CDS 45.0%
!! TTTGTGTTGCTTGCAGGCTT+CGG + chr4_2:51597259-51597278 Msa0605650:intron 45.0%
CGTGAAACTGGAAGATCAAG+AGG + chr4_2:51597582-51597601 Msa0605650:CDS 45.0%
AGGGATGAACGGTTCAGATA+TGG + chr4_2:51597656-51597675 Msa0605650:CDS 45.0%
ACGGTTCAGATATGGATGGA+AGG + chr4_2:51597664-51597683 Msa0605650:CDS 45.0%
! GTTGAGTACCGTTGCTTCGT+CGG + chr4_2:51597155-51597174 Msa0605650:CDS 50.0%
!! GGTCTGTTTGTGTTGCTTGC+AGG + chr4_2:51597253-51597272 Msa0605650:intron 50.0%
!! TGGTGGTTATGGTGGTGGTA+GGG + chr4_2:51597851-51597870 Msa0605650:CDS 50.0%
CATCTCCACCACCATAACCA+CGG - chr4_2:51597878-51597897 None:intergenic 50.0%
!! GGTTATGGTGGTGGAGATGA+TGG + chr4_2:51597879-51597898 Msa0605650:CDS 50.0%
!! TATGGTGGTGGAGATGATGG+TGG + chr4_2:51597882-51597901 Msa0605650:CDS 50.0%
TATTCTTCACGCGGTGGAGA+TGG + chr4_2:51597912-51597931 Msa0605650:CDS 50.0%
! GTTGAGTACCGTTGCTTCGT+CGG + chr4_2:51597155-51597174 Msa0605650:CDS 50.0%
!! GGTCTGTTTGTGTTGCTTGC+AGG + chr4_2:51597253-51597272 Msa0605650:intron 50.0%
!! TGGTGGTTATGGTGGTGGTA+GGG + chr4_2:51597851-51597870 Msa0605650:CDS 50.0%
CATCTCCACCACCATAACCA+CGG - chr4_2:51597878-51597897 None:intergenic 50.0%
!! GGTTATGGTGGTGGAGATGA+TGG + chr4_2:51597879-51597898 Msa0605650:CDS 50.0%
!! TATGGTGGTGGAGATGATGG+TGG + chr4_2:51597882-51597901 Msa0605650:CDS 50.0%
TATTCTTCACGCGGTGGAGA+TGG + chr4_2:51597912-51597931 Msa0605650:CDS 50.0%
CAAGACCTCCGACGAAGCAA+CGG - chr4_2:51597166-51597185 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG + chr4_2:51597169-51597188 Msa0605650:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG + chr4_2:51597264-51597283 Msa0605650:intron 55.0%
TTCTGCTGTAACCACCCTCG+CGG - chr4_2:51597794-51597813 None:intergenic 55.0%
GGTGGTTACAGCAGAAGCAG+TGG + chr4_2:51597798-51597817 Msa0605650:CDS 55.0%
GGTTACAGCAGAAGCAGTGG+TGG + chr4_2:51597801-51597820 Msa0605650:CDS 55.0%
TACAGCAGAAGCAGTGGTGG+TGG + chr4_2:51597804-51597823 Msa0605650:CDS 55.0%
!! AGTGGTGGTGGAGGTGGTTA+TGG + chr4_2:51597816-51597835 Msa0605650:CDS 55.0%
!! GTGGTGGTTATGGTGGTGGT+AGG + chr4_2:51597850-51597869 Msa0605650:CDS 55.0%
GGTTCCCGCTATTCTTCACG+CGG + chr4_2:51597903-51597922 Msa0605650:CDS 55.0%
TCCACCGCGTGAAGAATAGC+GGG - chr4_2:51597910-51597929 None:intergenic 55.0%
CTCCACCGCGTGAAGAATAG+CGG - chr4_2:51597911-51597930 None:intergenic 55.0%
CAAGACCTCCGACGAAGCAA+CGG - chr4_2:51597166-51597185 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG + chr4_2:51597169-51597188 Msa0605650:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG + chr4_2:51597264-51597283 Msa0605650:intron 55.0%
TTCTGCTGTAACCACCCTCG+CGG - chr4_2:51597794-51597813 None:intergenic 55.0%
GGTGGTTACAGCAGAAGCAG+TGG + chr4_2:51597798-51597817 Msa0605650:CDS 55.0%
GGTTACAGCAGAAGCAGTGG+TGG + chr4_2:51597801-51597820 Msa0605650:CDS 55.0%
TACAGCAGAAGCAGTGGTGG+TGG + chr4_2:51597804-51597823 Msa0605650:CDS 55.0%
!! AGTGGTGGTGGAGGTGGTTA+TGG + chr4_2:51597816-51597835 Msa0605650:CDS 55.0%
!! GTGGTGGTTATGGTGGTGGT+AGG + chr4_2:51597850-51597869 Msa0605650:CDS 55.0%
GGTTCCCGCTATTCTTCACG+CGG + chr4_2:51597903-51597922 Msa0605650:CDS 55.0%
TCCACCGCGTGAAGAATAGC+GGG - chr4_2:51597910-51597929 None:intergenic 55.0%
CTCCACCGCGTGAAGAATAG+CGG - chr4_2:51597911-51597930 None:intergenic 55.0%
! GAGTACCGTTGCTTCGTCGG+AGG + chr4_2:51597158-51597177 Msa0605650:CDS 60.0%
GCTTCGTCGGAGGTCTTGCA+TGG + chr4_2:51597168-51597187 Msa0605650:CDS 60.0%
CTCGAGAGCTTCGCTATCGG+TGG - chr4_2:51597195-51597214 None:intergenic 60.0%
GTGAACGAAGCTCAGAGCCG+TGG + chr4_2:51597696-51597715 Msa0605650:CDS 60.0%
GAAGCTCAGAGCCGTGGTAG+CGG + chr4_2:51597702-51597721 Msa0605650:CDS 60.0%
AAGCTCAGAGCCGTGGTAGC+GGG + chr4_2:51597703-51597722 Msa0605650:CDS 60.0%
CACCATATCCACCGCCACCA+CGG - chr4_2:51597740-51597759 None:intergenic 60.0%
! GGTGGATATGGTGGAGGTGG+TGG + chr4_2:51597747-51597766 Msa0605650:CDS 60.0%
! GGATATGGTGGAGGTGGTGG+TGG + chr4_2:51597750-51597769 Msa0605650:CDS 60.0%
!! GGTGGAGGTGGTGGTGGATA+TGG + chr4_2:51597756-51597775 Msa0605650:CDS 60.0%
!! GGAGGTGGTGGTGGATATGG+CGG + chr4_2:51597759-51597778 Msa0605650:CDS 60.0%
!! AGCAGAAGCAGTGGTGGTGG+AGG + chr4_2:51597807-51597826 Msa0605650:CDS 60.0%
!! AGAAGCAGTGGTGGTGGAGG+TGG + chr4_2:51597810-51597829 Msa0605650:CDS 60.0%
!! GGTGGTGGAGGTGGTTATGG+AGG + chr4_2:51597819-51597838 Msa0605650:CDS 60.0%
GGTGGAGGTGGTTATGGAGG+AGG + chr4_2:51597822-51597841 Msa0605650:CDS 60.0%
GGAGGTGGTTATGGAGGAGG+AGG + chr4_2:51597825-51597844 Msa0605650:CDS 60.0%
GGTGGTTATGGAGGAGGAGG+TGG + chr4_2:51597828-51597847 Msa0605650:CDS 60.0%
GGTTATGGAGGAGGAGGTGG+TGG + chr4_2:51597831-51597850 Msa0605650:CDS 60.0%
! TATGGAGGAGGAGGTGGTGG+TGG + chr4_2:51597834-51597853 Msa0605650:CDS 60.0%
!! GGAGGAGGTGGTGGTGGTTA+TGG + chr4_2:51597840-51597859 Msa0605650:CDS 60.0%
!! GGAGGTGGTGGTGGTTATGG+TGG + chr4_2:51597843-51597862 Msa0605650:CDS 60.0%
!! GGTGGTGGTGGTTATGGTGG+TGG + chr4_2:51597846-51597865 Msa0605650:CDS 60.0%
!! TATGGTGGTGGTAGGGACCG+TGG + chr4_2:51597858-51597877 Msa0605650:CDS 60.0%
GGTGGTAGGGACCGTGGTTA+TGG + chr4_2:51597864-51597883 Msa0605650:CDS 60.0%
GGTAGGGACCGTGGTTATGG+TGG + chr4_2:51597867-51597886 Msa0605650:CDS 60.0%
AGGGACCGTGGTTATGGTGG+TGG + chr4_2:51597870-51597889 Msa0605650:CDS 60.0%
TCCCGCTATTCTTCACGCGG+TGG + chr4_2:51597906-51597925 Msa0605650:CDS 60.0%
TCTTCACGCGGTGGAGATGG+TGG + chr4_2:51597915-51597934 Msa0605650:CDS 60.0%
!! GTGGAGATGGTGGAGGAAGC+TGG + chr4_2:51597925-51597944 Msa0605650:CDS 60.0%
! GAGTACCGTTGCTTCGTCGG+AGG + chr4_2:51597158-51597177 Msa0605650:CDS 60.0%
GCTTCGTCGGAGGTCTTGCA+TGG + chr4_2:51597168-51597187 Msa0605650:CDS 60.0%
CTCGAGAGCTTCGCTATCGG+TGG - chr4_2:51597195-51597214 None:intergenic 60.0%
GTGAACGAAGCTCAGAGCCG+TGG + chr4_2:51597696-51597715 Msa0605650:CDS 60.0%
GAAGCTCAGAGCCGTGGTAG+CGG + chr4_2:51597702-51597721 Msa0605650:CDS 60.0%
AAGCTCAGAGCCGTGGTAGC+GGG + chr4_2:51597703-51597722 Msa0605650:CDS 60.0%
CACCATATCCACCGCCACCA+CGG - chr4_2:51597740-51597759 None:intergenic 60.0%
! GGTGGATATGGTGGAGGTGG+TGG + chr4_2:51597747-51597766 Msa0605650:CDS 60.0%
! GGATATGGTGGAGGTGGTGG+TGG + chr4_2:51597750-51597769 Msa0605650:CDS 60.0%
!! GGTGGAGGTGGTGGTGGATA+TGG + chr4_2:51597756-51597775 Msa0605650:CDS 60.0%
!! GGAGGTGGTGGTGGATATGG+CGG + chr4_2:51597759-51597778 Msa0605650:CDS 60.0%
!! AGCAGAAGCAGTGGTGGTGG+AGG + chr4_2:51597807-51597826 Msa0605650:CDS 60.0%
!! AGAAGCAGTGGTGGTGGAGG+TGG + chr4_2:51597810-51597829 Msa0605650:CDS 60.0%
!! GGTGGTGGAGGTGGTTATGG+AGG + chr4_2:51597819-51597838 Msa0605650:CDS 60.0%
GGTGGAGGTGGTTATGGAGG+AGG + chr4_2:51597822-51597841 Msa0605650:CDS 60.0%
GGAGGTGGTTATGGAGGAGG+AGG + chr4_2:51597825-51597844 Msa0605650:CDS 60.0%
GGTGGTTATGGAGGAGGAGG+TGG + chr4_2:51597828-51597847 Msa0605650:CDS 60.0%
GGTTATGGAGGAGGAGGTGG+TGG + chr4_2:51597831-51597850 Msa0605650:CDS 60.0%
! TATGGAGGAGGAGGTGGTGG+TGG + chr4_2:51597834-51597853 Msa0605650:CDS 60.0%
!! GGAGGAGGTGGTGGTGGTTA+TGG + chr4_2:51597840-51597859 Msa0605650:CDS 60.0%
!! GGAGGTGGTGGTGGTTATGG+TGG + chr4_2:51597843-51597862 Msa0605650:CDS 60.0%
!! GGTGGTGGTGGTTATGGTGG+TGG + chr4_2:51597846-51597865 Msa0605650:CDS 60.0%
!! TATGGTGGTGGTAGGGACCG+TGG + chr4_2:51597858-51597877 Msa0605650:CDS 60.0%
GGTGGTAGGGACCGTGGTTA+TGG + chr4_2:51597864-51597883 Msa0605650:CDS 60.0%
GGTAGGGACCGTGGTTATGG+TGG + chr4_2:51597867-51597886 Msa0605650:CDS 60.0%
AGGGACCGTGGTTATGGTGG+TGG + chr4_2:51597870-51597889 Msa0605650:CDS 60.0%
TCCCGCTATTCTTCACGCGG+TGG + chr4_2:51597906-51597925 Msa0605650:CDS 60.0%
TCTTCACGCGGTGGAGATGG+TGG + chr4_2:51597915-51597934 Msa0605650:CDS 60.0%
!! GTGGAGATGGTGGAGGAAGC+TGG + chr4_2:51597925-51597944 Msa0605650:CDS 60.0%
! AGCTCAGAGCCGTGGTAGCG+GGG + chr4_2:51597704-51597723 Msa0605650:CDS 65.0%
!! CGTGGTGGCGGTGGATATGG+TGG + chr4_2:51597738-51597757 Msa0605650:CDS 65.0%
! GGTGGCGGTGGATATGGTGG+AGG + chr4_2:51597741-51597760 Msa0605650:CDS 65.0%
! GGCGGTGGATATGGTGGAGG+TGG + chr4_2:51597744-51597763 Msa0605650:CDS 65.0%
!! GGTGGTGGTGGATATGGCGG+AGG + chr4_2:51597762-51597781 Msa0605650:CDS 65.0%
!! GGTGGTGGATATGGCGGAGG+TGG + chr4_2:51597765-51597784 Msa0605650:CDS 65.0%
! TCACGCGGTGGAGATGGTGG+AGG + chr4_2:51597918-51597937 Msa0605650:CDS 65.0%
! AGCTCAGAGCCGTGGTAGCG+GGG + chr4_2:51597704-51597723 Msa0605650:CDS 65.0%
!! CGTGGTGGCGGTGGATATGG+TGG + chr4_2:51597738-51597757 Msa0605650:CDS 65.0%
! GGTGGCGGTGGATATGGTGG+AGG + chr4_2:51597741-51597760 Msa0605650:CDS 65.0%
! GGCGGTGGATATGGTGGAGG+TGG + chr4_2:51597744-51597763 Msa0605650:CDS 65.0%
!! GGTGGTGGTGGATATGGCGG+AGG + chr4_2:51597762-51597781 Msa0605650:CDS 65.0%
!! GGTGGTGGATATGGCGGAGG+TGG + chr4_2:51597765-51597784 Msa0605650:CDS 65.0%
! TCACGCGGTGGAGATGGTGG+AGG + chr4_2:51597918-51597937 Msa0605650:CDS 65.0%
!! GCTCAGAGCCGTGGTAGCGG+GGG + chr4_2:51597705-51597724 Msa0605650:CDS 70.0%
CTCCTCCACCCCCGCTACCA+CGG - chr4_2:51597716-51597735 None:intergenic 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG + chr4_2:51597735-51597754 Msa0605650:CDS 70.0%
!! GCTCAGAGCCGTGGTAGCGG+GGG + chr4_2:51597705-51597724 Msa0605650:CDS 70.0%
CTCCTCCACCCCCGCTACCA+CGG - chr4_2:51597716-51597735 None:intergenic 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG + chr4_2:51597735-51597754 Msa0605650:CDS 70.0%
!! CAGAGCCGTGGTAGCGGGGG+TGG + chr4_2:51597708-51597727 Msa0605650:CDS 75.0%
!! AGCCGTGGTAGCGGGGGTGG+AGG + chr4_2:51597711-51597730 Msa0605650:CDS 75.0%
!! CGTGGTAGCGGGGGTGGAGG+AGG + chr4_2:51597714-51597733 Msa0605650:CDS 75.0%
GGTGGAGGAGGCCGTGGTGG+CGG + chr4_2:51597726-51597745 Msa0605650:CDS 75.0%
!! CAGAGCCGTGGTAGCGGGGG+TGG + chr4_2:51597708-51597727 Msa0605650:CDS 75.0%
!! AGCCGTGGTAGCGGGGGTGG+AGG + chr4_2:51597711-51597730 Msa0605650:CDS 75.0%
!! CGTGGTAGCGGGGGTGGAGG+AGG + chr4_2:51597714-51597733 Msa0605650:CDS 75.0%
GGTGGAGGAGGCCGTGGTGG+CGG + chr4_2:51597726-51597745 Msa0605650:CDS 75.0%
! AGCGGGGGTGGAGGAGGCCG+TGG + chr4_2:51597720-51597739 Msa0605650:CDS 80.0%
! GGGGGTGGAGGAGGCCGTGG+TGG + chr4_2:51597723-51597742 Msa0605650:CDS 80.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG + chr4_2:51597729-51597748 Msa0605650:CDS 80.0%
! TGGCGGAGGTGGTCGCCGCG+AGG + chr4_2:51597776-51597795 Msa0605650:CDS 80.0%
! GGCGGAGGTGGTCGCCGCGA+GGG + chr4_2:51597777-51597796 Msa0605650:CDS 80.0%
! GGAGGTGGTCGCCGCGAGGG+TGG + chr4_2:51597780-51597799 Msa0605650:CDS 80.0%
! AGCGGGGGTGGAGGAGGCCG+TGG + chr4_2:51597720-51597739 Msa0605650:CDS 80.0%
! GGGGGTGGAGGAGGCCGTGG+TGG + chr4_2:51597723-51597742 Msa0605650:CDS 80.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG + chr4_2:51597729-51597748 Msa0605650:CDS 80.0%
! TGGCGGAGGTGGTCGCCGCG+AGG + chr4_2:51597776-51597795 Msa0605650:CDS 80.0%
! GGCGGAGGTGGTCGCCGCGA+GGG + chr4_2:51597777-51597796 Msa0605650:CDS 80.0%
! GGAGGTGGTCGCCGCGAGGG+TGG + chr4_2:51597780-51597799 Msa0605650:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 51597070 51597956 51597070 ID=Msa0605650;Name=Msa0605650
chr4_2 mRNA 51597070 51597956 51597070 ID=Msa0605650-mRNA-1;Parent=Msa0605650;Name=Msa0605650-mRNA-1;_AED=0.14;_eAED=0.14;_QI=70|1|0.5|1|1|1|2|0|166
chr4_2 exon 51597070 51597253 51597070 ID=Msa0605650-mRNA-1:exon:12390;Parent=Msa0605650-mRNA-1
chr4_2 exon 51597570 51597956 51597570 ID=Msa0605650-mRNA-1:exon:12391;Parent=Msa0605650-mRNA-1
chr4_2 five_prime_UTR 51597070 51597139 51597070 ID=Msa0605650-mRNA-1:five_prime_utr;Parent=Msa0605650-mRNA-1
chr4_2 CDS 51597140 51597253 51597140 ID=Msa0605650-mRNA-1:cds;Parent=Msa0605650-mRNA-1
chr4_2 CDS 51597570 51597956 51597570 ID=Msa0605650-mRNA-1:cds;Parent=Msa0605650-mRNA-1
Gene Sequence

>Msa0605650

ATGGCTTCTGCAGATGTTGAGTACCGTTGCTTCGTCGGAGGTCTTGCATGGGCCACCGATAGCGAAGCTCTCGAGAAAGCCTTCTCTCAATACGGAGAAATCATCGATTCGAAGATTATCAACGATCGTGAAACTGGAAGATCAAGAGGATTTGGATTTGTGACATTTGCTGATGAGAAATCGATGAGAGATGCTATTGAAGGGATGAACGGTTCAGATATGGATGGAAGGAACATTACTGTGAACGAAGCTCAGAGCCGTGGTAGCGGGGGTGGAGGAGGCCGTGGTGGCGGTGGATATGGTGGAGGTGGTGGTGGATATGGCGGAGGTGGTCGCCGCGAGGGTGGTTACAGCAGAAGCAGTGGTGGTGGAGGTGGTTATGGAGGAGGAGGTGGTGGTGGTTATGGTGGTGGTAGGGACCGTGGTTATGGTGGTGGAGATGATGGTGGTTCCCGCTATTCTTCACGCGGTGGAGATGGTGGAGGAAGCTGGAGAAGTTAG
Protein sequence

>Msa0605650

MASADVEYRCFVGGLAWATDSEALEKAFSQYGEIIDSKIINDRETGRSRGFGFVTFADEKSMRDAIEGMNGSDMDGRNITVNEAQSRGSGGGGGRGGGGYGGGGGGYGGGGRREGGYSRSSGGGGGYGGGGGGGYGGGRDRGYGGGDDGGSRYSSRGGDGGGSWRS*