AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0423920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423920 MtrunA17_Chr8g0386311 75.676 37 9 0 5 41 44 80 2.07e-11 56.6
Msa0423920 MtrunA17_Chr3g0132111 69.231 39 12 0 3 41 41 79 6.32e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATAGACCGTTGATGTTTC+AGG 0.247801 3_2:+74032912 None:intergenic
TATCTGATGTGGTTTAGTTT+GGG 0.288954 3_2:-74031827 Msa0423920:three_prime_UTR
GTATCTGATGTGGTTTAGTT+TGG 0.306306 3_2:-74031828 Msa0423920:three_prime_UTR
GCAACTTTCAAAGTCATTGA+TGG 0.343752 3_2:-74032937 Msa0423920:CDS
TATCATGAGAAATTCTTTCA+TGG 0.364175 3_2:-74034909 Msa0423920:five_prime_UTR
ACCACATAATATTAACACTA+TGG 0.382317 3_2:+74034658 None:intergenic
TTGTAACTTGGTTTCGTCAA+TGG 0.403372 3_2:+74034382 None:intergenic
AAGAAAAGAGAAGGGTAAGA+AGG 0.409014 3_2:+74034613 None:intergenic
AGGGAACACATGCAGGCAAA+TGG 0.416639 3_2:+74034633 None:intergenic
TTGAAAGTTGCAATGATGTC+AGG 0.435539 3_2:+74032949 None:intergenic
AGAAAAGAGAAGGGTAAGAA+GGG 0.437360 3_2:+74034614 None:intergenic
TGCAGGGAATACAAAAGAAA+AGG 0.439924 3_2:-74030876 Msa0423920:intron
TATATAGAGGTGGAAGGAAA+TGG 0.445027 3_2:-74030815 Msa0423920:three_prime_UTR
TCCATAGTGTTAATATTATG+TGG 0.451170 3_2:-74034659 Msa0423920:five_prime_UTR
TTTGCAGTTAATATCCTTGA+GGG 0.481204 3_2:-74032268 Msa0423920:intron
GGATGCAATGGTGGTGGAGA+AGG 0.489038 3_2:+74034573 None:intergenic
GGAGGTTCTACTTTGTAACT+TGG 0.490121 3_2:+74034370 None:intergenic
GGTAAGAAGGGAACACATGC+AGG 0.497320 3_2:+74034626 None:intergenic
CAATTTCTAACTGATTCAAA+TGG 0.497571 3_2:-74032852 Msa0423920:intron
ATCTGATGTGGTTTAGTTTG+GGG 0.498692 3_2:-74031826 Msa0423920:three_prime_UTR
GGGAACACATGCAGGCAAAT+GGG 0.500313 3_2:+74034634 None:intergenic
CAATGGAAGCTTGTGATGGC+AGG 0.504710 3_2:+74034399 None:intergenic
AAGAGAGAAGGTGGATGCAA+TGG 0.514890 3_2:+74034561 None:intergenic
TGAAAGAATTTCTCATGATA+CGG 0.521690 3_2:+74034912 None:intergenic
ATTTGAAGGAATGTTGTTTG+AGG 0.522652 3_2:-74034287 Msa0423920:intron
GAAGGTGGATGCAATGGTGG+TGG 0.540169 3_2:+74034567 None:intergenic
GTGAATCTAGGCGAGGGAAG+TGG 0.550537 3_2:+74032234 None:intergenic
AACGTACAGTAGGTGAATCT+AGG 0.553854 3_2:+74032222 None:intergenic
TCAATGACAAAGCTATATAG+AGG 0.556884 3_2:-74030828 Msa0423920:three_prime_UTR
AGAGAAGGTGGATGCAATGG+TGG 0.562967 3_2:+74034564 None:intergenic
TTCTTTGTCAGTATCTGATG+TGG 0.581703 3_2:-74031838 Msa0423920:intron
TCGTCAATGGAAGCTTGTGA+TGG 0.582356 3_2:+74034395 None:intergenic
ACGAGACTAAAATTTATGAC+CGG 0.596956 3_2:+74034698 None:intergenic
TGGTTGTCTCATCACCCTCA+AGG 0.606403 3_2:+74032254 None:intergenic
CAAAGCTATATAGAGGTGGA+AGG 0.610830 3_2:-74030821 Msa0423920:three_prime_UTR
AATGGTGGTGGAGAAGGAAA+AGG 0.611622 3_2:+74034579 None:intergenic
CAGTAGGTGAATCTAGGCGA+GGG 0.620624 3_2:+74032228 None:intergenic
AACATTCCTTCAAATAGCTG+AGG 0.630227 3_2:+74034295 None:intergenic
CTAGAAGCAACAGTTCTGCA+AGG 0.631238 3_2:-74031798 Msa0423920:three_prime_UTR
GTTAGACATGATGAAAACAA+AGG 0.640821 3_2:-74031767 Msa0423920:three_prime_UTR
ATGACAAAGCTATATAGAGG+TGG 0.642666 3_2:-74030825 Msa0423920:three_prime_UTR
ACAGTAGGTGAATCTAGGCG+AGG 0.644049 3_2:+74032227 None:intergenic
GATGGTGCCTGAAACATCAA+CGG 0.665675 3_2:-74032919 Msa0423920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAATATTATTTAAAATT+TGG + chr3_2:74033618-74033637 None:intergenic 0.0%
!!! TTTAAATAATATTTTTAATT+AGG - chr3_2:74033621-74033640 Msa0423920:intron 0.0%
!! AATACAAATAAAAAATGTTT+GGG + chr3_2:74030888-74030907 None:intergenic 10.0%
!! AAATACAAATAAAAAATGTT+TGG + chr3_2:74030889-74030908 None:intergenic 10.0%
!!! CAAATTTAGAATTAAAAAAA+TGG + chr3_2:74031600-74031619 None:intergenic 10.0%
!! ATAAACAAAATATAAACTAA+TGG + chr3_2:74031648-74031667 None:intergenic 10.0%
!!! TGTTATTATTATTATTTTCT+TGG - chr3_2:74031709-74031728 Msa0423920:intron 10.0%
!!! GTTATTATTATTATTTTCTT+GGG - chr3_2:74031710-74031729 Msa0423920:intron 10.0%
!! GTTAATATAATTTAACAAAA+AGG + chr3_2:74032368-74032387 None:intergenic 10.0%
!!! GTTTATCATTTTTTTTTTAT+TGG - chr3_2:74032501-74032520 Msa0423920:intron 10.0%
!!! TGTCATTTTATAATAAAAAT+TGG - chr3_2:74034288-74034307 Msa0423920:five_prime_UTR 10.0%
!!! CAGTTAATATTTTTATTTGT+GGG + chr3_2:74031321-74031340 None:intergenic 15.0%
!!! ATTAATCACAATTTTTTTTC+TGG - chr3_2:74031539-74031558 Msa0423920:intron 15.0%
!! ATTATTATTTCCATTTGAAT+CGG + chr3_2:74031960-74031979 None:intergenic 15.0%
!!! GTTTTAATTCTTATCAAATT+TGG + chr3_2:74032058-74032077 None:intergenic 15.0%
!! TGATAAGAATTAAAACTTTA+AGG - chr3_2:74032062-74032081 Msa0423920:intron 15.0%
!!! TATCATTTTTTTTTTATTGG+TGG - chr3_2:74032504-74032523 Msa0423920:intron 15.0%
!! TTTGTTTGTTTATTTGATAT+TGG + chr3_2:74033069-74033088 None:intergenic 15.0%
!!! ATGTTTTTCATTTAGAAATT+CGG + chr3_2:74033928-74033947 None:intergenic 15.0%
!! ATATTATTAGTAATCACATT+AGG + chr3_2:74033956-74033975 None:intergenic 15.0%
!!! ACTAATAATATGACAAATTT+TGG - chr3_2:74033965-74033984 Msa0423920:intron 15.0%
!!! TTTGAAGTAAAAAAACATAT+GGG + chr3_2:74034091-74034110 None:intergenic 15.0%
!!! TTTTGAAGTAAAAAAACATA+TGG + chr3_2:74034092-74034111 None:intergenic 15.0%
!! AGAATTAACATACATTAAAA+TGG - chr3_2:74034551-74034570 Msa0423920:five_prime_UTR 15.0%
!!! CAATGTCATTATATTTTAAC+CGG - chr3_2:74030930-74030949 Msa0423920:intron 20.0%
!! AAAAAAGAAAGAAAAGAGAA+GGG + chr3_2:74031045-74031064 None:intergenic 20.0%
!! AAAAAAAGAAAGAAAAGAGA+AGG + chr3_2:74031046-74031065 None:intergenic 20.0%
!!! TAAAGATAAGAGTTTTATAG+AGG + chr3_2:74031176-74031195 None:intergenic 20.0%
!!! CCAGTTAATATTTTTATTTG+TGG + chr3_2:74031322-74031341 None:intergenic 20.0%
!! CCACAAATAAAAATATTAAC+TGG - chr3_2:74031319-74031338 Msa0423920:intron 20.0%
!! AATGTACATATAAAAGAAGA+TGG + chr3_2:74031501-74031520 None:intergenic 20.0%
!!! ATATTTGTGATTTAGCATTT+TGG - chr3_2:74031758-74031777 Msa0423920:intron 20.0%
!! AAAACAAAATTAACACTCAA+AGG + chr3_2:74031887-74031906 None:intergenic 20.0%
!! TTATTATTTCCATTTGAATC+GGG + chr3_2:74031959-74031978 None:intergenic 20.0%
!!! CATTTTTGGATATAATTTAC+TGG - chr3_2:74032236-74032255 Msa0423920:exon 20.0%
!! GTTAAATTATATTAACACCT+TGG - chr3_2:74032372-74032391 Msa0423920:intron 20.0%
!! TGGATTACTTAATTATGATT+TGG - chr3_2:74032845-74032864 Msa0423920:intron 20.0%
!! TTTAGTTATTTGTCAACAAA+GGG + chr3_2:74033104-74033123 None:intergenic 20.0%
!!! ATTAGTCTATGAGTATAATT+GGG + chr3_2:74033249-74033268 None:intergenic 20.0%
!!! AATTAGTCTATGAGTATAAT+TGG + chr3_2:74033250-74033269 None:intergenic 20.0%
!! TATACTCATAGACTAATTTA+TGG - chr3_2:74033252-74033271 Msa0423920:intron 20.0%
!! AGATACAATAAAACAGTAAA+AGG + chr3_2:74033739-74033758 None:intergenic 20.0%
!! AGAGTTAAAAGATTAAATTC+AGG + chr3_2:74034115-74034134 None:intergenic 20.0%
!!! TACATGTAATTTAGAGTTAA+AGG + chr3_2:74034162-74034181 None:intergenic 20.0%
!!! TAAATTACATGTACCTTTTT+TGG - chr3_2:74034169-74034188 Msa0423920:intron 20.0%
!!! GAAAGTAACATATTTTCTAA+GGG + chr3_2:74034246-74034265 None:intergenic 20.0%
!!! AGAAAGTAACATATTTTCTA+AGG + chr3_2:74034247-74034266 None:intergenic 20.0%
!!! TGTTACTTTCTTTTTAAATG+TGG - chr3_2:74034256-74034275 Msa0423920:intron 20.0%
!!! AATATTTTTTGTATTCAGCT+GGG + chr3_2:74034402-74034421 None:intergenic 20.0%
!!! TAATATTTTTTGTATTCAGC+TGG + chr3_2:74034403-74034422 None:intergenic 20.0%
!! AAAATATTATAGATTGCTTC+AGG - chr3_2:74034414-74034433 Msa0423920:five_prime_UTR 20.0%
!!! TTCTAATATACATCCTTTTT+AGG + chr3_2:74034535-74034554 None:intergenic 20.0%
!! TAATATACATCCTTCTAATT+AGG + chr3_2:74034578-74034597 None:intergenic 20.0%
!! GGATGTATATTAGAAATATA+AGG - chr3_2:74034586-74034605 Msa0423920:five_prime_UTR 20.0%
! TGAAAGAATTTCTCATGATA+CGG + chr3_2:74030738-74030757 None:intergenic 25.0%
! TATCATGAGAAATTCTTTCA+TGG - chr3_2:74030738-74030757 Msa0423920:three_prime_UTR 25.0%
! TCCATAGTGTTAATATTATG+TGG - chr3_2:74030988-74031007 Msa0423920:intron 25.0%
! ACCACATAATATTAACACTA+TGG + chr3_2:74030992-74031011 None:intergenic 25.0%
!!! GGTTTTATTTTAGTATGAAG+CGG + chr3_2:74031131-74031150 None:intergenic 25.0%
!!! ATTTTTTAGACTGTCATTTC+TGG - chr3_2:74031217-74031236 Msa0423920:intron 25.0%
!! TTTTATTGTGAATATCAAGG+AGG + chr3_2:74031298-74031317 None:intergenic 25.0%
!!! GTTCTTTTTCACTATTTTTG+AGG - chr3_2:74031390-74031409 Msa0423920:intron 25.0%
!!! CTATATTTTTACAAGAAACG+AGG + chr3_2:74031469-74031488 None:intergenic 25.0%
! ATGCTAAATCACAAATATAG+AGG + chr3_2:74031756-74031775 None:intergenic 25.0%
! TTCTAATGACATGTTAATGA+AGG - chr3_2:74031849-74031868 Msa0423920:intron 25.0%
! AAGATGATAATCTGATCTAA+AGG - chr3_2:74031983-74032002 Msa0423920:intron 25.0%
! ACATAATAATCAAATGCGTA+AGG + chr3_2:74032093-74032112 None:intergenic 25.0%
! TTACGCATTTGATTATTATG+TGG - chr3_2:74032092-74032111 Msa0423920:intron 25.0%
!! GTAAATCAACGATTTTTGAA+TGG - chr3_2:74032153-74032172 Msa0423920:intron 25.0%
!! GTTTAGAAAAGTTGCATTTT+TGG - chr3_2:74032222-74032241 Msa0423920:intron 25.0%
!!! TTTTTTATGCAATAAGTCCA+AGG + chr3_2:74032392-74032411 None:intergenic 25.0%
! TTCAAACATACTTTATGACT+TGG - chr3_2:74032454-74032473 Msa0423920:intron 25.0%
!! AAAATGTTAATAGAAAGTGC+AGG + chr3_2:74032620-74032639 None:intergenic 25.0%
!! CAATTTCTAACTGATTCAAA+TGG - chr3_2:74032795-74032814 Msa0423920:intron 25.0%
! CTTTAGTTATTTGTCAACAA+AGG + chr3_2:74033105-74033124 None:intergenic 25.0%
! GCGAAGAAACATAATTAAAA+TGG + chr3_2:74033460-74033479 None:intergenic 25.0%
!! ATGACAATTTTTGTATCATC+TGG - chr3_2:74033571-74033590 Msa0423920:intron 25.0%
! ATAGCTTATTCTATGACAAT+TGG + chr3_2:74033673-74033692 None:intergenic 25.0%
! TTTAGAAATTCGGTAGAAAA+AGG + chr3_2:74033918-74033937 None:intergenic 25.0%
!!! TATGACAAATTTTGGTTTCT+CGG - chr3_2:74033973-74033992 Msa0423920:intron 25.0%
! TTCAAAAAGATTAAGACTTC+AGG + chr3_2:74034067-74034086 None:intergenic 25.0%
!!! GTTCATCAAAAAAGTTTTGA+AGG + chr3_2:74034476-74034495 None:intergenic 25.0%
! TTAAAATGGTCCTAATTAGA+AGG - chr3_2:74034565-74034584 Msa0423920:five_prime_UTR 25.0%
ACGAGACTAAAATTTATGAC+CGG + chr3_2:74030952-74030971 None:intergenic 30.0%
TGATGAAAAAAGAAGAGAGA+AGG + chr3_2:74031101-74031120 None:intergenic 30.0%
!!! TTATTTTAGTATGAAGCGGT+CGG + chr3_2:74031127-74031146 None:intergenic 30.0%
!! AGAGTTTTATAGAGGAGAAA+GGG + chr3_2:74031168-74031187 None:intergenic 30.0%
!! AAGAGTTTTATAGAGGAGAA+AGG + chr3_2:74031169-74031188 None:intergenic 30.0%
!!! GGGTTTTATTGTGAATATCA+AGG + chr3_2:74031301-74031320 None:intergenic 30.0%
! ATTTGAAGGAATGTTGTTTG+AGG - chr3_2:74031360-74031379 Msa0423920:intron 30.0%
! GTTTTATCCGATGCAAAAAT+TGG + chr3_2:74031421-74031440 None:intergenic 30.0%
GAGTATCAACAAAGACAATA+TGG + chr3_2:74031932-74031951 None:intergenic 30.0%
TTGGATATAATTTACTGGTG+TGG - chr3_2:74032241-74032260 Msa0423920:CDS 30.0%
TGGATATAATTTACTGGTGT+GGG - chr3_2:74032242-74032261 Msa0423920:CDS 30.0%
TGGTGGTAAATAAGTCTAAA+TGG - chr3_2:74032521-74032540 Msa0423920:intron 30.0%
! CTGATAACTTTGTTTCTTCT+TGG - chr3_2:74032651-74032670 Msa0423920:intron 30.0%
!! ACTTTGTTTCTTCTTGGTTT+TGG - chr3_2:74032657-74032676 Msa0423920:intron 30.0%
!!! TTTCTTCTTGGTTTTGGATT+AGG - chr3_2:74032663-74032682 Msa0423920:intron 30.0%
!!! TTCTTCTTGGTTTTGGATTA+GGG - chr3_2:74032664-74032683 Msa0423920:intron 30.0%
ATTTACGAACTTCTTCACTA+TGG - chr3_2:74032820-74032839 Msa0423920:intron 30.0%
!! TTGATATTGGTGCTAAATTC+AGG + chr3_2:74033056-74033075 None:intergenic 30.0%
TTTGTCAACAAAGGGAAATT+TGG + chr3_2:74033096-74033115 None:intergenic 30.0%
GATGATTAATTCGATCAGTT+TGG - chr3_2:74033133-74033152 Msa0423920:intron 30.0%
! ATCAGTTTGGTAATATCTGT+TGG - chr3_2:74033146-74033165 Msa0423920:intron 30.0%
! TCAGTTTGGTAATATCTGTT+GGG - chr3_2:74033147-74033166 Msa0423920:intron 30.0%
ACTTATCAAGAACATAAAGC+AGG + chr3_2:74033201-74033220 None:intergenic 30.0%
! ATGTAAGCATTCCTTTTAAG+AGG + chr3_2:74033286-74033305 None:intergenic 30.0%
! TTTTGCAGTTAATATCCTTG+AGG - chr3_2:74033378-74033397 Msa0423920:intron 30.0%
TTTGCAGTTAATATCCTTGA+GGG - chr3_2:74033379-74033398 Msa0423920:intron 30.0%
! CACATCTATTTGAAATGCTT+GGG + chr3_2:74033513-74033532 None:intergenic 30.0%
! TCACATCTATTTGAAATGCT+TGG + chr3_2:74033514-74033533 None:intergenic 30.0%
AGCATTTCAAATAGATGTGA+TGG - chr3_2:74033514-74033533 Msa0423920:intron 30.0%
GCATTTCAAATAGATGTGAT+GGG - chr3_2:74033515-74033534 Msa0423920:intron 30.0%
TTCTATGACAATTGGCATAA+GGG + chr3_2:74033665-74033684 None:intergenic 30.0%
ATTCTATGACAATTGGCATA+AGG + chr3_2:74033666-74033685 None:intergenic 30.0%
! TATCTGATGTGGTTTAGTTT+GGG - chr3_2:74033820-74033839 Msa0423920:intron 30.0%
GTTAGACATGATGAAAACAA+AGG - chr3_2:74033880-74033899 Msa0423920:intron 30.0%
!! GATTAAGACTTCAGGTTTTT+CGG + chr3_2:74034059-74034078 None:intergenic 30.0%
! CTTGAACAATAAACTTTTCC+TGG - chr3_2:74034319-74034338 Msa0423920:five_prime_UTR 30.0%
TGAGACAACAACAAATATGA+AGG + chr3_2:74034693-74034712 None:intergenic 30.0%
!!! TCCATCTTCAAATTTTTTGC+AGG - chr3_2:74034754-74034773 Msa0423920:five_prime_UTR 30.0%
!!! CCATCTTCAAATTTTTTGCA+GGG - chr3_2:74034755-74034774 Msa0423920:five_prime_UTR 30.0%
TCAATGACAAAGCTATATAG+AGG - chr3_2:74034819-74034838 Msa0423920:five_prime_UTR 30.0%
AGAAAAGAGAAGGGTAAGAA+GGG + chr3_2:74031036-74031055 None:intergenic 35.0%
AAGAAAAGAGAAGGGTAAGA+AGG + chr3_2:74031037-74031056 None:intergenic 35.0%
TGAAAAAAGAAGAGAGAAGG+TGG + chr3_2:74031098-74031117 None:intergenic 35.0%
!! AGTTTTATAGAGGAGAAAGG+GGG + chr3_2:74031166-74031185 None:intergenic 35.0%
!! GAGTTTTATAGAGGAGAAAG+GGG + chr3_2:74031167-74031186 None:intergenic 35.0%
TTGTAACTTGGTTTCGTCAA+TGG + chr3_2:74031268-74031287 None:intergenic 35.0%
! TTTTCACCTCAGCTATTTGA+AGG - chr3_2:74031346-74031365 Msa0423920:intron 35.0%
AACATTCCTTCAAATAGCTG+AGG + chr3_2:74031355-74031374 None:intergenic 35.0%
! GGATCTACCAATTTTTGCAT+CGG - chr3_2:74031411-74031430 Msa0423920:intron 35.0%
TACTCATATCCCGATTCAAA+TGG - chr3_2:74031947-74031966 Msa0423920:intron 35.0%
CTCATCATAACATGGGAAAT+TGG + chr3_2:74032019-74032038 None:intergenic 35.0%
AAAGGCTCTCATCATAACAT+GGG + chr3_2:74032026-74032045 None:intergenic 35.0%
TAAAGGCTCTCATCATAACA+TGG + chr3_2:74032027-74032046 None:intergenic 35.0%
ATTCTTATCAAATTTGGCCG+AGG + chr3_2:74032052-74032071 None:intergenic 35.0%
! ATGCTTTCAAGTACCTTTCT+TGG - chr3_2:74032118-74032137 Msa0423920:intron 35.0%
! AAGTACCTTTCTTGGACTTT+TGG - chr3_2:74032126-74032145 Msa0423920:intron 35.0%
GCTGTAAGTGAGGTAAATTT+GGG - chr3_2:74032476-74032495 Msa0423920:intron 35.0%
!!! TCTTCTTGGTTTTGGATTAG+GGG - chr3_2:74032665-74032684 Msa0423920:intron 35.0%
TTGAAAGTTGCAATGATGTC+AGG + chr3_2:74032701-74032720 None:intergenic 35.0%
GCAACTTTCAAAGTCATTGA+TGG - chr3_2:74032710-74032729 Msa0423920:intron 35.0%
! CTATGAAGTTTTTCGTCGAA+TGG - chr3_2:74032752-74032771 Msa0423920:intron 35.0%
AACTTTGCTCGCTAATATCT+TGG - chr3_2:74032896-74032915 Msa0423920:CDS 35.0%
ACTTTGCTCGCTAATATCTT+GGG - chr3_2:74032897-74032916 Msa0423920:CDS 35.0%
TGGTTGTTTAGCCTCTTAAA+AGG - chr3_2:74033272-74033291 Msa0423920:intron 35.0%
GAGAGAAAAAAACGTACAGT+AGG + chr3_2:74033438-74033457 None:intergenic 35.0%
! AATAGATGTGATGGGACAAA+AGG - chr3_2:74033523-74033542 Msa0423920:intron 35.0%
TTCTTTGTCAGTATCTGATG+TGG - chr3_2:74033809-74033828 Msa0423920:intron 35.0%
GTATCTGATGTGGTTTAGTT+TGG - chr3_2:74033819-74033838 Msa0423920:intron 35.0%
!! ATCTGATGTGGTTTAGTTTG+GGG - chr3_2:74033821-74033840 Msa0423920:intron 35.0%
!!! ATGTACCTTTTTTGGACTCT+TGG - chr3_2:74034177-74034196 Msa0423920:intron 35.0%
! ACAATAAACTTTTCCTGGTG+TGG - chr3_2:74034324-74034343 Msa0423920:five_prime_UTR 35.0%
! CAATAAACTTTTCCTGGTGT+GGG - chr3_2:74034325-74034344 Msa0423920:five_prime_UTR 35.0%
CTTGCTTCTCAGATATACAA+AGG + chr3_2:74034616-74034635 None:intergenic 35.0%
CCCTGCAAAAAATTTGAAGA+TGG + chr3_2:74034758-74034777 None:intergenic 35.0%
TGCAGGGAATACAAAAGAAA+AGG - chr3_2:74034771-74034790 Msa0423920:five_prime_UTR 35.0%
ATGACAAAGCTATATAGAGG+TGG - chr3_2:74034822-74034841 Msa0423920:five_prime_UTR 35.0%
TATATAGAGGTGGAAGGAAA+TGG - chr3_2:74034832-74034851 Msa0423920:five_prime_UTR 35.0%
TTGAAAAAGATCTGCAACGA+AGG - chr3_2:74034858-74034877 Msa0423920:five_prime_UTR 35.0%
! GTTTTATAGAGGAGAAAGGG+GGG + chr3_2:74031165-74031184 None:intergenic 40.0%
GGAGGTTCTACTTTGTAACT+TGG + chr3_2:74031280-74031299 None:intergenic 40.0%
CAACACCAAAAGTCCAAGAA+AGG + chr3_2:74032134-74032153 None:intergenic 40.0%
! TTATGACTTGGCTGTAAGTG+AGG - chr3_2:74032466-74032485 Msa0423920:intron 40.0%
GGCTGTAAGTGAGGTAAATT+TGG - chr3_2:74032475-74032494 Msa0423920:intron 40.0%
!!! CTTCTTGGTTTTGGATTAGG+GGG - chr3_2:74032666-74032685 Msa0423920:intron 40.0%
!!! TTCTTGGTTTTGGATTAGGG+GGG - chr3_2:74032667-74032686 Msa0423920:intron 40.0%
TCATAGACCGTTGATGTTTC+AGG + chr3_2:74032738-74032757 None:intergenic 40.0%
CGAACTTCTTCACTATGGAA+TGG - chr3_2:74032825-74032844 Msa0423920:intron 40.0%
AACGTACAGTAGGTGAATCT+AGG + chr3_2:74033428-74033447 None:intergenic 40.0%
CTACGCATCAAGCTTGATAA+AGG + chr3_2:74033696-74033715 None:intergenic 40.0%
ACACACCAAGAGTCCAAAAA+AGG + chr3_2:74034185-74034204 None:intergenic 40.0%
GGTGTGTTGTAAATCTTCAG+TGG - chr3_2:74034198-74034217 Msa0423920:intron 40.0%
!!! CCTTTTTAGGTGTACTTTGC+AGG + chr3_2:74034522-74034541 None:intergenic 40.0%
CCTGCAAAGTACACCTAAAA+AGG - chr3_2:74034519-74034538 Msa0423920:five_prime_UTR 40.0%
CTGAGAAGCAAGAAATGAAG+AGG - chr3_2:74034624-74034643 Msa0423920:five_prime_UTR 40.0%
AATGAAGAGGTCAACCACTA+TGG - chr3_2:74034637-74034656 Msa0423920:five_prime_UTR 40.0%
TGTACTACTCCAAACCATAG+TGG + chr3_2:74034654-74034673 None:intergenic 40.0%
CAAAGCTATATAGAGGTGGA+AGG - chr3_2:74034826-74034845 Msa0423920:five_prime_UTR 40.0%
!! AATGGTGGTGGAGAAGGAAA+AGG + chr3_2:74031071-74031090 None:intergenic 45.0%
AAGAGAGAAGGTGGATGCAA+TGG + chr3_2:74031089-74031108 None:intergenic 45.0%
TCGTCAATGGAAGCTTGTGA+TGG + chr3_2:74031255-74031274 None:intergenic 45.0%
TGATGAGAGCCTTTACACCT+CGG - chr3_2:74032032-74032051 Msa0423920:intron 45.0%
CAAATTTGGCCGAGGTGTAA+AGG + chr3_2:74032044-74032063 None:intergenic 45.0%
!!! TCTTGGTTTTGGATTAGGGG+GGG - chr3_2:74032668-74032687 Msa0423920:intron 45.0%
GATGGTGCCTGAAACATCAA+CGG - chr3_2:74032728-74032747 Msa0423920:intron 45.0%
CTAGAAGCAACAGTTCTGCA+AGG - chr3_2:74033849-74033868 Msa0423920:intron 45.0%
AGAGGTCAACCACTATGGTT+TGG - chr3_2:74034642-74034661 Msa0423920:five_prime_UTR 45.0%
GGGAACACATGCAGGCAAAT+GGG + chr3_2:74031016-74031035 None:intergenic 50.0%
AGGGAACACATGCAGGCAAA+TGG + chr3_2:74031017-74031036 None:intergenic 50.0%
GGTAAGAAGGGAACACATGC+AGG + chr3_2:74031024-74031043 None:intergenic 50.0%
AGAGAAGGTGGATGCAATGG+TGG + chr3_2:74031086-74031105 None:intergenic 50.0%
CAATGGAAGCTTGTGATGGC+AGG + chr3_2:74031251-74031270 None:intergenic 50.0%
TGGTTGTCTCATCACCCTCA+AGG + chr3_2:74033396-74033415 None:intergenic 50.0%
CAGTAGGTGAATCTAGGCGA+GGG + chr3_2:74033422-74033441 None:intergenic 50.0%
ACAGTAGGTGAATCTAGGCG+AGG + chr3_2:74033423-74033442 None:intergenic 50.0%
TTTCCTGGTGTGGGTACTTC+AGG - chr3_2:74034334-74034353 Msa0423920:five_prime_UTR 50.0%
! TATCCTGAAGTACCCACACC+AGG + chr3_2:74034340-74034359 None:intergenic 50.0%
!! GGATGCAATGGTGGTGGAGA+AGG + chr3_2:74031077-74031096 None:intergenic 55.0%
GAAGGTGGATGCAATGGTGG+TGG + chr3_2:74031083-74031102 None:intergenic 55.0%
GAAAGGGGGGATCATGATGG+TGG + chr3_2:74031152-74031171 None:intergenic 55.0%
GGAGAAAGGGGGGATCATGA+TGG + chr3_2:74031155-74031174 None:intergenic 55.0%
GTGAATCTAGGCGAGGGAAG+TGG + chr3_2:74033416-74033435 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 74030728 74034941 74030728 ID=Msa0423920;Name=Msa0423920
chr3_2 mRNA 74030728 74034941 74030728 ID=Msa0423920-mRNA-1;Parent=Msa0423920;Name=Msa0423920-mRNA-1;_AED=0.01;_eAED=0.01;_QI=662|1|1|1|0.25|0|5|263|55
chr3_2 exon 74030728 74030893 74030728 ID=Msa0423920-mRNA-1:exon:18629;Parent=Msa0423920-mRNA-1
chr3_2 exon 74031764 74031849 74031764 ID=Msa0423920-mRNA-1:exon:18628;Parent=Msa0423920-mRNA-1
chr3_2 exon 74032229 74032283 74032229 ID=Msa0423920-mRNA-1:exon:18627;Parent=Msa0423920-mRNA-1
chr3_2 exon 74032853 74032984 74032853 ID=Msa0423920-mRNA-1:exon:18626;Parent=Msa0423920-mRNA-1
chr3_2 exon 74034288 74034941 74034288 ID=Msa0423920-mRNA-1:exon:18625;Parent=Msa0423920-mRNA-1
chr3_2 five_prime_UTR 74034288 74034941 74034288 ID=Msa0423920-mRNA-1:five_prime_utr;Parent=Msa0423920-mRNA-1
chr3_2 five_prime_UTR 74032977 74032984 74032977 ID=Msa0423920-mRNA-1:five_prime_utr;Parent=Msa0423920-mRNA-1
chr3_2 CDS 74032853 74032976 74032853 ID=Msa0423920-mRNA-1:cds;Parent=Msa0423920-mRNA-1
chr3_2 CDS 74032240 74032283 74032240 ID=Msa0423920-mRNA-1:cds;Parent=Msa0423920-mRNA-1
chr3_2 three_prime_UTR 74032229 74032239 74032229 ID=Msa0423920-mRNA-1:three_prime_utr;Parent=Msa0423920-mRNA-1
chr3_2 three_prime_UTR 74031764 74031849 74031764 ID=Msa0423920-mRNA-1:three_prime_utr;Parent=Msa0423920-mRNA-1
chr3_2 three_prime_UTR 74030728 74030893 74030728 ID=Msa0423920-mRNA-1:three_prime_utr;Parent=Msa0423920-mRNA-1
Gene Sequence

>Msa0423920

ATGAACCTGACATCATTGCAACTTTCAAAGTCATTGATGGTGCCTGAAACATCAACGGTCTATGAAGTTTTTCGTCGAATGGCTGTTTGCAGAGTAGATGCTCAATTTCTAACTGATTCAAATGTTAATATCCTTGAGGGTGATGAGACAACCACTTCCCTCGCCTAG
Protein sequence

>Msa0423920

MNLTSLQLSKSLMVPETSTVYEVFRRMAVCRVDAQFLTDSNVNILEGDETTTSLA*