AlfalfaGEDB | Msa0728620

M. sativa cultivar zhongmu-4 / Msa0728620

KEGG: -
GO: GO:0003674 GO:0003824 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0010150 GO:0016491 GO:0016679 GO:0016682 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055114 GO:0090693 GO:0099402
PFAM: PF14226.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0728620	KAG5051595.1	57.277	213	66	1	1	188	89	301	7.64e-81	252

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0728620	sp\|Q39224\|SRG1_ARATH	50.980	102	50	0	1	102	99	200	8.13e-31	118
Msa0728620	sp\|Q39224\|SRG1_ARATH	50.000	80	40	0	103	182	279	358	5.11e-19	86.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0728620	A0A445LP14	57.277	213	66	1	1	188	89	301	3.65e-81	252

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0616560	Msa0728620	-0.803443	3.477919e-49	-8.615850e-47
Msa0655990	Msa0728620	-0.805796	1.123079e-49	-8.615850e-47
Msa0699920	Msa0728620	-0.802249	6.136433e-49	-8.615850e-47
Msa0710070	Msa0728620	0.807725	4.392972e-50	-8.615850e-47
Msa0728620	Msa0812760	0.800764	1.236677e-48	-8.615850e-47
Msa0728620	Msa1087530	0.803921	2.767244e-49	-8.615850e-47
Msa0728620	Msa1205130	0.815698	8.089419e-52	-8.615850e-47
Msa0728620	Msa1438270	0.814927	1.200858e-51	-8.615850e-47
Msa0728620	Msa1454290	-0.804064	2.584685e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0728620	MtrunA17_Chr5g0421101	94.175	103	6	0	1	103	91	193	2.78e-67	209
Msa0728620	MtrunA17_Chr5g0421101	97.674	86	2	0	103	188	269	354	9.81e-54	174
Msa0728620	MtrunA17_Chr5g0423221	79.612	103	21	0	1	103	115	217	2.37e-57	184
Msa0728620	MtrunA17_Chr5g0423221	67.442	86	28	0	103	188	294	379	3.30e-34	124
Msa0728620	MtrunA17_Chr5g0423201	82.692	104	17	1	1	103	91	194	4.52e-57	183
Msa0728620	MtrunA17_Chr5g0423201	66.279	86	29	0	103	188	270	355	9.33e-35	125
Msa0728620	MtrunA17_Chr5g0421041	65.049	103	36	0	1	103	89	191	5.40e-45	152
Msa0728620	MtrunA17_Chr5g0421041	66.279	86	29	0	103	188	267	352	1.16e-35	127
Msa0728620	MtrunA17_Chr5g0421011	66.667	102	34	0	1	102	91	192	5.76e-45	152
Msa0728620	MtrunA17_Chr5g0421011	62.651	83	31	0	103	185	269	351	5.68e-31	115

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0728620	AT4G25310.2	39.394	231	91	1	1	182	95	325	1.71e-55	178
Msa0728620	AT4G25300.1	40.972	144	73	2	1	143	98	230	2.93e-32	119
Msa0728620	AT4G25300.1	47.500	80	42	0	103	182	277	356	1.45e-20	87.8
Msa0728620	AT1G17020.1	50.980	102	50	0	1	102	99	200	8.26e-32	118
Msa0728620	AT1G17020.1	50.000	80	40	0	103	182	279	358	5.20e-20	86.3
Msa0728620	AT4G25310.1	50.000	102	51	0	1	102	95	196	3.19e-31	116
Msa0728620	AT4G25310.1	50.000	80	40	0	103	182	274	353	3.67e-21	89.4
Msa0728620	AT1G17010.1	48.039	102	53	0	1	102	100	201	5.15e-28	107
Msa0728620	AT1G17010.1	52.439	82	39	0	103	184	279	360	4.30e-23	94.7
Msa0728620	AT1G78550.2	40.351	114	67	1	1	113	21	134	8.57e-25	97.8

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Find 108 sgRNAs with CRISPR-Local

Find 356 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGGTGGAGGAAGTGA+AGG	0.597899	5_1:+33508325	Msa0728620:five_prime_UTR
AAACACCAAATGATGTGCAG+GGG	0.677901	5_1:+33509788	Msa0728620:CDS
AAACTAGCTGTCACGATAAT+TGG	0.284486	5_1:+33510081	Msa0728620:CDS
AAAGAAACCCCAGCCTCTGC+AGG	0.453935	5_1:-33507240	None:intergenic
AACATTGGTGTGTGAATCCG+AGG	0.605949	5_1:+33508402	Msa0728620:five_prime_UTR
AACCCCAGCCTCTGCAGGCA+TGG	0.523413	5_1:-33507235	None:intergenic
AACGGAAGGAACCAAGAGAG+AGG	0.659896	5_1:-33507051	None:intergenic
AATCATGCATAGATGTTGTA+AGG	0.464909	5_1:+33510870	Msa0728620:CDS
AATTTATGCAGGTCGATAAC+AGG	0.430269	5_1:-33507176	None:intergenic
AATTTCTTCTTCTCTTCCAT+TGG	0.427457	5_1:-33509760	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATATCAAATGGCTATGAA+GGG	+	chr5_1:33507026-33507045	Msa0728620:five_prime_UTR	30.0%
TTGTGTTAGAAACAGCAATA+GGG	-	chr5_1:33507139-33507158	None:intergenic	30.0%
TTTGTGTTAGAAACAGCAAT+AGG	-	chr5_1:33507140-33507159	None:intergenic	30.0%
AATACCAATTCAGAACAGAA+AGG	+	chr5_1:33507473-33507492	Msa0728620:intron	30.0%
AAAATTCCATTTAGTGCGTA+GGG	+	chr5_1:33508039-33508058	Msa0728620:intron	30.0%
CATCTTCATTTCGAACAATT+TGG	+	chr5_1:33508179-33508198	Msa0728620:five_prime_UTR	30.0%
ATTTGGAGGTTTGATAAGTA+TGG	+	chr5_1:33508196-33508215	Msa0728620:five_prime_UTR	30.0%
TTTGGAGGTTTGATAAGTAT+GGG	+	chr5_1:33508197-33508216	Msa0728620:five_prime_UTR	30.0%
TATCATTGAAAAATTGGTGG+AGG	+	chr5_1:33508316-33508335	Msa0728620:five_prime_UTR	30.0%
TAGATTACAACTTGCAACAT+TGG	+	chr5_1:33508387-33508406	Msa0728620:five_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	33506945	33511784	33506945	ID=Msa0728620;Name=Msa0728620
chr5_1	mRNA	33506945	33511784	33506945	ID=Msa0728620-mRNA-1;Parent=Msa0728620;Name=Msa0728620-mRNA-1;_AED=0.34;_eAED=0.34;_QI=946\|0.8\|0.83\|1\|0.6\|0.5\|6\|229\|188
chr5_1	exon	33506945	33507265	33506945	ID=Msa0728620-mRNA-1:exon:10702;Parent=Msa0728620-mRNA-1
chr5_1	exon	33508120	33508705	33508120	ID=Msa0728620-mRNA-1:exon:10703;Parent=Msa0728620-mRNA-1
chr5_1	exon	33509688	33509935	33509688	ID=Msa0728620-mRNA-1:exon:10704;Parent=Msa0728620-mRNA-1
chr5_1	exon	33510047	33510143	33510047	ID=Msa0728620-mRNA-1:exon:10705;Parent=Msa0728620-mRNA-1
chr5_1	exon	33510656	33511030	33510656	ID=Msa0728620-mRNA-1:exon:10706;Parent=Msa0728620-mRNA-1
chr5_1	exon	33511670	33511784	33511670	ID=Msa0728620-mRNA-1:exon:10707;Parent=Msa0728620-mRNA-1
chr5_1	five_prime_UTR	33506945	33507265	33506945	ID=Msa0728620-mRNA-1:five_prime_utr;Parent=Msa0728620-mRNA-1
chr5_1	five_prime_UTR	33508120	33508705	33508120	ID=Msa0728620-mRNA-1:five_prime_utr;Parent=Msa0728620-mRNA-1
chr5_1	five_prime_UTR	33509688	33509726	33509688	ID=Msa0728620-mRNA-1:five_prime_utr;Parent=Msa0728620-mRNA-1
chr5_1	CDS	33509727	33509935	33509727	ID=Msa0728620-mRNA-1:cds;Parent=Msa0728620-mRNA-1
chr5_1	CDS	33510047	33510143	33510047	ID=Msa0728620-mRNA-1:cds;Parent=Msa0728620-mRNA-1
chr5_1	CDS	33510656	33510916	33510656	ID=Msa0728620-mRNA-1:cds;Parent=Msa0728620-mRNA-1
chr5_1	three_prime_UTR	33510917	33511030	33510917	ID=Msa0728620-mRNA-1:three_prime_utr;Parent=Msa0728620-mRNA-1
chr5_1	three_prime_UTR	33511670	33511784	33511670	ID=Msa0728620-mRNA-1:three_prime_utr;Parent=Msa0728620-mRNA-1

Gene Sequence

>Msa0728620

ATGAAGACAGGTGTTGAACAATTTCTCAAACTTCCAATGGAAGAGAAGAAGAAATTTTGGCAAACACCAAATGATGTGCAGGGGTTTGGTCAGTTGTTTGTTGTATCTAATGAACAGAAGCTAGAATGGGCAGACATGTTCTACATTAACACTTTCCCACCGGATGCAAGGCATCCACGTCTAATTCCTAATATTCCCGAACCATTCAGAGATCATCTAGAGAGTTATTGTTTAGAATTGAAAAAACTAGCTGTCACGATAATTGGTCGTATGGAGAAAGCTCTAAAGATAAAAAGCAACGAACTAATATTAACTAACGGAATTTACCGAAGCATTGAACATCGAGCAACAATCAATTCAATGAATGAGAGGATATCAATTGCCACATTTCATAGGACTCAAATGAGCAAAATTCTAGGTCCAACACCAGACCTTATTACTGCTGAAAGACCTGCTTTGTTCAAAAGAATTAGGGCAGTTGATTACCTTAATGGATTCATGTCACGTGAGCTACAAGGGAAATCATGCATAGATGTTGTAAGGATTCAAAATGATATTGGTAAATAA

Protein sequence

>Msa0728620

MKTGVEQFLKLPMEEKKKFWQTPNDVQGFGQLFVVSNEQKLEWADMFYINTFPPDARHPRLIPNIPEPFRDHLESYCLELKKLAVTIIGRMEKALKIKSNELILTNGIYRSIEHRATINSMNERISIATFHRTQMSKILGPTPDLITAERPALFKRIRAVDYLNGFMSRELQGKSCIDVVRIQNDIGK*