AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0710070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTATAATTTGAATATTTAT+TGG 0.149686 5_1:-1683284 None:intergenic
ATGATTTGTAGTATCAAATT+TGG 0.159254 5_1:+1683700 Msa0710070:three_prime_UTR
TCACAGTTCTTTGCAATTTC+AGG 0.167918 5_1:+1680532 Msa0710070:CDS
AAATATAGATTTGGATTTAA+TGG 0.180402 5_1:+1683435 Msa0710070:CDS
TATTTCCTCAACACCTAAAA+TGG 0.217388 5_1:-1683608 None:intergenic
AATGCAAAGGGTAGAGTATA+TGG 0.262241 5_1:+1683214 Msa0710070:CDS
GAGAGGTTGAGGCGCGTTTA+AGG 0.273052 5_1:+1683329 Msa0710070:CDS
TTAGTTGATTCTTAGATCCT+TGG 0.322858 5_1:-1681839 None:intergenic
ACAAACAATCGATTCCATTA+AGG 0.346122 5_1:+1683372 Msa0710070:CDS
ATTTGCATGTTTAGCAAATA+CGG 0.348024 5_1:-1683416 None:intergenic
TAAACATGCAAATATAGATT+TGG 0.354681 5_1:+1683426 Msa0710070:CDS
AAAGGTTTGTGACCCCTTCC+TGG 0.359594 5_1:-1682018 None:intergenic
TAGTTGATTCTTAGATCCTT+GGG 0.378840 5_1:-1681838 None:intergenic
ACTAAAGCATGAACTTGAAT+TGG 0.381714 5_1:+1681857 Msa0710070:CDS
TAGAGTATATGGGCTTGGTT+TGG 0.416967 5_1:+1683225 Msa0710070:CDS
ATTAGTGTATCATAATCAAT+AGG 0.448901 5_1:-1683169 None:intergenic
CTAAACAAGTGCAGAACAAA+AGG 0.449735 5_1:+1681949 Msa0710070:intron
TGAGTCTCAACCTCATCCTT+TGG 0.456749 5_1:+1683111 Msa0710070:CDS
TATTGATGGTTTATGAATGT+AGG 0.459697 5_1:+1683538 Msa0710070:three_prime_UTR
ATGTGGAAGAATAATATTGA+TGG 0.474708 5_1:+1683524 Msa0710070:exon
GTCTCAACCTCATCCTTTGG+AGG 0.479026 5_1:+1683114 Msa0710070:CDS
GGATTTAGACGTTGAGCCAC+GGG 0.479351 5_1:-1683263 None:intergenic
CAACCTCATCCTTTGGAGGC+TGG 0.487170 5_1:+1683118 Msa0710070:CDS
CAAGTGCAGAACAAAAGGTT+AGG 0.488681 5_1:+1681954 Msa0710070:intron
AAGGGTAGAGTATATGGGCT+TGG 0.493623 5_1:+1683220 Msa0710070:CDS
TGTATCATAATCAATAGGCT+TGG 0.504722 5_1:-1683164 None:intergenic
GTATCATAATCAATAGGCTT+GGG 0.509867 5_1:-1683163 None:intergenic
TGGTATGAGTGGAGAGGTTG+AGG 0.514731 5_1:+1683318 Msa0710070:CDS
GTATATGGGCTTGGTTTGGA+TGG 0.514998 5_1:+1683229 Msa0710070:CDS
ATGCAAAGGGTAGAGTATAT+GGG 0.526831 5_1:+1683215 Msa0710070:CDS
ACACTAATGATTGAAGCTGT+TGG 0.537396 5_1:+1683184 Msa0710070:CDS
GATGGCAGCATGCGCACCCG+TGG 0.550034 5_1:+1683247 Msa0710070:CDS
CTAATGATTGAAGCTGTTGG+AGG 0.555236 5_1:+1683187 Msa0710070:CDS
TTAAATAGCTCTCCCTTGTG+TGG 0.555638 5_1:-1681984 None:intergenic
TGCAAATGTTGGTGCTGATG+TGG 0.559498 5_1:+1683507 Msa0710070:CDS
TGGATTTAGACGTTGAGCCA+CGG 0.559998 5_1:-1683264 None:intergenic
AGTAATCGTGATTCAGATGT+CGG 0.565082 5_1:+1681801 Msa0710070:CDS
AGTTGATCTCTTATCAACAA+AGG 0.578886 5_1:-1682036 None:intergenic
GGAGGTTCATTGCACACCCA+AGG 0.595430 5_1:+1681822 Msa0710070:CDS
TTACCAGCCTCCAAAGGATG+AGG 0.600248 5_1:-1683121 None:intergenic
CGTGGAAGCAATGCAAATGT+TGG 0.616233 5_1:+1683496 Msa0710070:CDS
TCATCATTACCAGCCTCCAA+AGG 0.621367 5_1:-1683127 None:intergenic
AATTATCTTAAGTAGATGTG+TGG 0.631134 5_1:+1681773 Msa0710070:intron
TGTTGGAGGAAGAAATGCAA+AGG 0.632509 5_1:+1683201 Msa0710070:CDS
GATATTGCACGTGGTATGAG+TGG 0.632896 5_1:+1683307 Msa0710070:CDS
TCTGTTGATAATGCTACTCG+TGG 0.635589 5_1:+1683478 Msa0710070:CDS
GTTGGAGGAAGAAATGCAAA+GGG 0.639504 5_1:+1683202 Msa0710070:CDS
GTTAGGAAGAATTCCACACA+AGG 0.649819 5_1:+1681971 Msa0710070:CDS
AATTATACTGATATTGCACG+TGG 0.677934 5_1:+1683298 Msa0710070:CDS
AATCGTGATTCAGATGTCGG+AGG 0.698344 5_1:+1681804 Msa0710070:CDS
TGCACGTGGTATGAGTGGAG+AGG 0.729243 5_1:+1683312 Msa0710070:CDS
GAGGCTGGTAATGATGAGAG+TGG 0.750491 5_1:+1683133 Msa0710070:CDS
TTAGGAAGAATTCCACACAA+GGG 0.761441 5_1:+1681972 Msa0710070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTATAATTTGAATATTTAT+TGG - chr5_1:1683287-1683306 None:intergenic 10.0%
!!! ATTTTTTGATTTTGATGAAA+TGG + chr5_1:1680641-1680660 Msa0710070:intron 15.0%
!!! TTTTTTGATTTTGATGAAAT+GGG + chr5_1:1680642-1680661 Msa0710070:intron 15.0%
!!! TTTGTTGAAATTTTGATTTT+TGG + chr5_1:1680665-1680684 Msa0710070:intron 15.0%
!!! TACAACATTTTTTTTATGAA+GGG + chr5_1:1680915-1680934 Msa0710070:intron 15.0%
!! ATCATCAAAAAAAAAAAATC+TGG - chr5_1:1681698-1681717 None:intergenic 15.0%
!! AATAACTGAATAAATAAGAT+AGG - chr5_1:1683052-1683071 None:intergenic 15.0%
!! AAATATAGATTTGGATTTAA+TGG + chr5_1:1683435-1683454 Msa0710070:CDS 15.0%
!!! ATTAAAACTTTAACCATTTT+AGG + chr5_1:1683595-1683614 Msa0710070:three_prime_UTR 15.0%
!!! TAATTTCAAGCATTTTTTTG+TGG - chr5_1:1680839-1680858 Msa0710060:five_prime_UTR 20.0%
!!! GTACAACATTTTTTTTATGA+AGG + chr5_1:1680914-1680933 Msa0710070:intron 20.0%
!!! GTATTTTTGTTTTCATGTAA+AGG + chr5_1:1681028-1681047 Msa0710070:intron 20.0%
!! CTTCAATTTATAATCCAAAA+GGG - chr5_1:1681091-1681110 Msa0710060:five_prime_UTR 20.0%
!! ACTTCAATTTATAATCCAAA+AGG - chr5_1:1681092-1681111 Msa0710060:five_prime_UTR 20.0%
!!! ACATTTTAGCTTAAAATTAG+TGG - chr5_1:1681175-1681194 Msa0710060:five_prime_UTR 20.0%
!!! ACATTTTTTCTTTTGTTCAT+TGG - chr5_1:1681323-1681342 Msa0710060:five_prime_UTR 20.0%
!!! TAATCTGCAAATTGATAATT+TGG + chr5_1:1681436-1681455 Msa0710070:intron 20.0%
!! CATTGTATGAGAAAATAAAA+TGG - chr5_1:1681725-1681744 None:intergenic 20.0%
!! ATGCATATAATATCAACAAT+TGG + chr5_1:1682317-1682336 Msa0710070:intron 20.0%
!! TGAATATCAATGAAATCAAA+GGG - chr5_1:1682553-1682572 None:intergenic 20.0%
!! ATGAATATCAATGAAATCAA+AGG - chr5_1:1682554-1682573 None:intergenic 20.0%
!! TTGTTTGACAGATATATTAT+CGG + chr5_1:1682767-1682786 Msa0710070:intron 20.0%
!! ATTAGTGTATCATAATCAAT+AGG - chr5_1:1683172-1683191 None:intergenic 20.0%
!! TAAACATGCAAATATAGATT+TGG + chr5_1:1683426-1683445 Msa0710070:CDS 20.0%
!!! GAAACAATTTGATATTTTGT+AGG + chr5_1:1683560-1683579 Msa0710070:three_prime_UTR 20.0%
!!! ATGATTTGTAGTATCAAATT+TGG + chr5_1:1683700-1683719 Msa0710070:three_prime_UTR 20.0%
!!! AGAGTTTGTATTTTTGTTTC+TGG + chr5_1:1680692-1680711 Msa0710070:intron 25.0%
!! TGCATTTTCTGTCAAATTAA+AGG - chr5_1:1680727-1680746 Msa0710060:five_prime_UTR 25.0%
! CATATCGAGTATAAGTTAAT+AGG - chr5_1:1680755-1680774 Msa0710060:five_prime_UTR 25.0%
! CTATTAACTTATACTCGATA+TGG + chr5_1:1680753-1680772 Msa0710070:intron 25.0%
! ATATGGATTTGAATGTTTGA+GGG + chr5_1:1680770-1680789 Msa0710070:intron 25.0%
!! TAATACTGGTGTATGATTTT+AGG + chr5_1:1680977-1680996 Msa0710070:intron 25.0%
!! TTTAAAACTTTATGGCAAAC+AGG - chr5_1:1681127-1681146 Msa0710060:five_prime_UTR 25.0%
!! CATGTGCATTTAAAACTTTA+TGG - chr5_1:1681135-1681154 Msa0710060:five_prime_UTR 25.0%
! ATATCATTGCATTTATTTGC+TGG - chr5_1:1681227-1681246 Msa0710060:five_prime_UTR 25.0%
!! CTTTTATGATAGCTAAAAAG+TGG + chr5_1:1681349-1681368 Msa0710070:intron 25.0%
! TTCAAAGGGATGTTTATATT+TGG + chr5_1:1681532-1681551 Msa0710070:intron 25.0%
! AATTATCTTAAGTAGATGTG+TGG + chr5_1:1681773-1681792 Msa0710070:intron 25.0%
! TATTTAAAAAATGTCACACC+AGG + chr5_1:1682000-1682019 Msa0710070:CDS 25.0%
!!! TTGGCTTCTTTAATTTTACT+TGG + chr5_1:1682083-1682102 Msa0710070:intron 25.0%
!! AGACTAGTACTTAATCTTTT+GGG + chr5_1:1682130-1682149 Msa0710070:intron 25.0%
! GACATAAGAATAAAATTGCA+AGG - chr5_1:1682456-1682475 None:intergenic 25.0%
! CAAGAAAATATTGTCTACTA+GGG + chr5_1:1682517-1682536 Msa0710070:intron 25.0%
! TTAGTGACATATAGTGAAAA+TGG + chr5_1:1682573-1682592 Msa0710070:intron 25.0%
! CAAAAACTGAAGTAGATAAA+AGG - chr5_1:1682802-1682821 None:intergenic 25.0%
!! CTTTTATCTACTTCAGTTTT+TGG + chr5_1:1682800-1682819 Msa0710070:intron 25.0%
!!! CTTTTTTGTTATTGCAATAC+TGG + chr5_1:1682842-1682861 Msa0710070:intron 25.0%
! CATGTATTGCCTTTATAATT+TGG + chr5_1:1682949-1682968 Msa0710070:intron 25.0%
!!! CCTTTATAATTTGGTTTGTT+GGG + chr5_1:1682958-1682977 Msa0710070:intron 25.0%
! ATTTGCATGTTTAGCAAATA+CGG - chr5_1:1683419-1683438 None:intergenic 25.0%
! ATGTGGAAGAATAATATTGA+TGG + chr5_1:1683524-1683543 Msa0710070:exon 25.0%
!! TATTGATGGTTTATGAATGT+AGG + chr5_1:1683538-1683557 Msa0710070:three_prime_UTR 25.0%
!!! TTGATATCTGCTGTTATTTT+TGG + chr5_1:1683633-1683652 Msa0710070:three_prime_UTR 25.0%
AGAGAATATCACCTATATTG+AGG - chr5_1:1680589-1680608 Msa0710060:five_prime_UTR 30.0%
GATATGGATTTGAATGTTTG+AGG + chr5_1:1680769-1680788 Msa0710070:intron 30.0%
! GAGTAGTTTGAAATCAATGA+GGG + chr5_1:1680937-1680956 Msa0710070:intron 30.0%
ATTAAGATACAGTAGTGAGA+GGG + chr5_1:1681250-1681269 Msa0710070:intron 30.0%
TTAATGTTTCTTCGAAGAAG+AGG - chr5_1:1681499-1681518 None:intergenic 30.0%
AAAGGGATGTTTATATTTGG+TGG + chr5_1:1681535-1681554 Msa0710070:intron 30.0%
!!! TTTTTAGTTCATGTACTTGC+AGG + chr5_1:1681611-1681630 Msa0710070:intron 30.0%
!!! TTTTAGTTCATGTACTTGCA+GGG + chr5_1:1681612-1681631 Msa0710070:intron 30.0%
! TAGTTGATTCTTAGATCCTT+GGG - chr5_1:1681841-1681860 None:intergenic 30.0%
! TTAGTTGATTCTTAGATCCT+TGG - chr5_1:1681842-1681861 None:intergenic 30.0%
ACTAAAGCATGAACTTGAAT+TGG + chr5_1:1681857-1681876 Msa0710070:CDS 30.0%
AGTTGATCTCTTATCAACAA+AGG - chr5_1:1682039-1682058 None:intergenic 30.0%
ATATGTGAGTATCAAGCTAT+TGG + chr5_1:1682064-1682083 Msa0710070:intron 30.0%
!! TTTACTTGGTTTGTGTAAGT+AGG + chr5_1:1682097-1682116 Msa0710070:intron 30.0%
! GAGACTAGTACTTAATCTTT+TGG + chr5_1:1682129-1682148 Msa0710070:intron 30.0%
!! TTAATCTTTTGGGTCTGTTT+TGG + chr5_1:1682140-1682159 Msa0710070:intron 30.0%
!! TGAGATTTTGAGTATGAGAA+TGG - chr5_1:1682182-1682201 None:intergenic 30.0%
TTTGCACCAATTGCTATATT+CGG - chr5_1:1682210-1682229 None:intergenic 30.0%
ATAAATCCATCCAAAAGTCA+AGG - chr5_1:1682429-1682448 None:intergenic 30.0%
ATATACCTCTCAGATTCATT+GGG - chr5_1:1682487-1682506 None:intergenic 30.0%
! CCTAGTAGACAATATTTTCT+TGG - chr5_1:1682519-1682538 None:intergenic 30.0%
CCAAGAAAATATTGTCTACT+AGG + chr5_1:1682516-1682535 Msa0710070:intron 30.0%
GAAAAAATTCACAATGCCTA+CGG + chr5_1:1682688-1682707 Msa0710070:intron 30.0%
AGAAAAAATAAACAGACCGT+AGG - chr5_1:1682707-1682726 None:intergenic 30.0%
!!! TTTTGGCTTGCATTTATGTT+TGG + chr5_1:1682817-1682836 Msa0710070:intron 30.0%
!!! TTTTGTTATTGCAATACTGG+TGG + chr5_1:1682845-1682864 Msa0710070:intron 30.0%
TATGAATCTAGTAATGTCTG+AGG + chr5_1:1682881-1682900 Msa0710070:intron 30.0%
!! GCCTTTATAATTTGGTTTGT+TGG + chr5_1:1682957-1682976 Msa0710070:intron 30.0%
CCCAACAAACCAAATTATAA+AGG - chr5_1:1682961-1682980 None:intergenic 30.0%
TTTGTTGGGATGTTCTATTT+AGG + chr5_1:1682972-1682991 Msa0710070:intron 30.0%
TTAAGAAGGCAAATTAAGGA+GGG - chr5_1:1683012-1683031 None:intergenic 30.0%
TTTAAGAAGGCAAATTAAGG+AGG - chr5_1:1683013-1683032 None:intergenic 30.0%
CAGTTTAAGAAGGCAAATTA+AGG - chr5_1:1683016-1683035 None:intergenic 30.0%
GTATCATAATCAATAGGCTT+GGG - chr5_1:1683166-1683185 None:intergenic 30.0%
TGTATCATAATCAATAGGCT+TGG - chr5_1:1683167-1683186 None:intergenic 30.0%
AATTATACTGATATTGCACG+TGG + chr5_1:1683298-1683317 Msa0710070:CDS 30.0%
! ACAAACAATCGATTCCATTA+AGG + chr5_1:1683372-1683391 Msa0710070:CDS 30.0%
!! TTTAACCATTTTAGGTGTTG+AGG + chr5_1:1683603-1683622 Msa0710070:three_prime_UTR 30.0%
TATTTCCTCAACACCTAAAA+TGG - chr5_1:1683611-1683630 None:intergenic 30.0%
!!! TATCTGCTGTTATTTTTGGA+TGG + chr5_1:1683637-1683656 Msa0710070:three_prime_UTR 30.0%
TCACAGTTCTTTGCAATTTC+AGG + chr5_1:1680532-1680551 Msa0710070:CDS 35.0%
!! GTTCTTTGCAATTTCAGGTT+TGG + chr5_1:1680537-1680556 Msa0710070:intron 35.0%
TCAGCTTCATTCCTCAATAT+AGG + chr5_1:1680575-1680594 Msa0710070:intron 35.0%
! GGAGTAGTTTGAAATCAATG+AGG + chr5_1:1680936-1680955 Msa0710070:intron 35.0%
! GCTTTTCTACAATAATGCCT+TGG + chr5_1:1681061-1681080 Msa0710070:intron 35.0%
GATTAAGATACAGTAGTGAG+AGG + chr5_1:1681249-1681268 Msa0710070:intron 35.0%
TTAAGATACAGTAGTGAGAG+GGG + chr5_1:1681251-1681270 Msa0710070:intron 35.0%
GACTGCAGCAAAAATTAAAG+AGG - chr5_1:1681379-1681398 None:intergenic 35.0%
!! TGCAGTTGATTTGCATACAT+TGG + chr5_1:1681402-1681421 Msa0710070:intron 35.0%
AATCTGCTGCATAGTTTCAA+AGG + chr5_1:1681517-1681536 Msa0710070:intron 35.0%
ATCTGCTGCATAGTTTCAAA+GGG + chr5_1:1681518-1681537 Msa0710070:intron 35.0%
! GATGTTTATATTTGGTGGTC+TGG + chr5_1:1681540-1681559 Msa0710070:intron 35.0%
! TTATATTTGGTGGTCTGGTT+TGG + chr5_1:1681545-1681564 Msa0710070:intron 35.0%
AGTAATCGTGATTCAGATGT+CGG + chr5_1:1681801-1681820 Msa0710070:CDS 35.0%
CTAAACAAGTGCAGAACAAA+AGG + chr5_1:1681949-1681968 Msa0710070:intron 35.0%
TTAGGAAGAATTCCACACAA+GGG + chr5_1:1681972-1681991 Msa0710070:CDS 35.0%
TAAAAAATGTCACACCAGGA+AGG + chr5_1:1682004-1682023 Msa0710070:CDS 35.0%
AAAAAATGTCACACCAGGAA+GGG + chr5_1:1682005-1682024 Msa0710070:CDS 35.0%
ACATCTCCGAATATAGCAAT+TGG + chr5_1:1682201-1682220 Msa0710070:intron 35.0%
GAATATAGCAATTGGTGCAA+AGG + chr5_1:1682209-1682228 Msa0710070:intron 35.0%
GATGCATACAAACTCTACTT+TGG + chr5_1:1682362-1682381 Msa0710070:intron 35.0%
! TGTCTTTTGTCCTTGACTTT+TGG + chr5_1:1682416-1682435 Msa0710070:intron 35.0%
! TTTTGTCCTTGACTTTTGGA+TGG + chr5_1:1682420-1682439 Msa0710070:intron 35.0%
TCAGATTCATTGGGGAAAAA+TGG - chr5_1:1682478-1682497 None:intergenic 35.0%
TATACCTCTCAGATTCATTG+GGG - chr5_1:1682486-1682505 None:intergenic 35.0%
GATATACCTCTCAGATTCAT+TGG - chr5_1:1682488-1682507 None:intergenic 35.0%
!! ATTGTCTACTAGGGTGTTTT+TGG + chr5_1:1682526-1682545 Msa0710070:intron 35.0%
AGATATATTATCGGCAACTG+TGG + chr5_1:1682776-1682795 Msa0710070:intron 35.0%
TTATTGCAATACTGGTGGTT+TGG + chr5_1:1682850-1682869 Msa0710070:intron 35.0%
!! GTAATCGAGTGACTTGTTAT+AGG + chr5_1:1682923-1682942 Msa0710070:intron 35.0%
ACAAGCAACACAGTTTAAGA+AGG - chr5_1:1683026-1683045 None:intergenic 35.0%
ACACTAATGATTGAAGCTGT+TGG + chr5_1:1683184-1683203 Msa0710070:CDS 35.0%
AATGCAAAGGGTAGAGTATA+TGG + chr5_1:1683214-1683233 Msa0710070:CDS 35.0%
ATGCAAAGGGTAGAGTATAT+GGG + chr5_1:1683215-1683234 Msa0710070:CDS 35.0%
!!! TTTGTTTTGACGAGCCTTAA+TGG - chr5_1:1683389-1683408 None:intergenic 35.0%
GGAATGAAGCTGAGAAAGAA+AGG - chr5_1:1680568-1680587 Msa0710060:exon 40.0%
GAATGTTTGAGGGAAGAAAG+AGG + chr5_1:1680780-1680799 Msa0710070:intron 40.0%
CAACATCCAAAGCGTAATAC+TGG + chr5_1:1680963-1680982 Msa0710070:intron 40.0%
CATACACCAGTATTACGCTT+TGG - chr5_1:1680972-1680991 Msa0710060:five_prime_UTR 40.0%
TAATCCAAAAGGGACAACCA+AGG - chr5_1:1681081-1681100 Msa0710060:five_prime_UTR 40.0%
TCTTCGAAGAAGAGGAATAG+AGG - chr5_1:1681491-1681510 None:intergenic 40.0%
CAAGTGCAGAACAAAAGGTT+AGG + chr5_1:1681954-1681973 Msa0710070:intron 40.0%
GTTAGGAAGAATTCCACACA+AGG + chr5_1:1681971-1681990 Msa0710070:CDS 40.0%
TTAAATAGCTCTCCCTTGTG+TGG - chr5_1:1681987-1682006 None:intergenic 40.0%
AAAAATGTCACACCAGGAAG+GGG + chr5_1:1682006-1682025 Msa0710070:CDS 40.0%
! TTTTCCCCAATGAATCTGAG+AGG + chr5_1:1682479-1682498 Msa0710070:intron 40.0%
CTAATGATTGAAGCTGTTGG+AGG + chr5_1:1683187-1683206 Msa0710070:CDS 40.0%
TGTTGGAGGAAGAAATGCAA+AGG + chr5_1:1683201-1683220 Msa0710070:CDS 40.0%
GTTGGAGGAAGAAATGCAAA+GGG + chr5_1:1683202-1683221 Msa0710070:CDS 40.0%
! TAGAGTATATGGGCTTGGTT+TGG + chr5_1:1683225-1683244 Msa0710070:CDS 40.0%
!! TCTGTTGATAATGCTACTCG+TGG + chr5_1:1683478-1683497 Msa0710070:CDS 40.0%
! CTGGTGTATGATTTTAGGCG+TGG + chr5_1:1680982-1681001 Msa0710070:intron 45.0%
! AATGCCTTGGTTGTCCCTTT+TGG + chr5_1:1681074-1681093 Msa0710070:intron 45.0%
!! GTCTGGTTTGGTTGTTCTGT+AGG + chr5_1:1681557-1681576 Msa0710070:intron 45.0%
ATGTACTTGCAGGGAAGTTG+TGG + chr5_1:1681621-1681640 Msa0710070:intron 45.0%
AATCGTGATTCAGATGTCGG+AGG + chr5_1:1681804-1681823 Msa0710070:CDS 45.0%
TGAGTCTCAACCTCATCCTT+TGG + chr5_1:1683111-1683130 Msa0710070:CDS 45.0%
TCATCATTACCAGCCTCCAA+AGG - chr5_1:1683130-1683149 None:intergenic 45.0%
AAGGGTAGAGTATATGGGCT+TGG + chr5_1:1683220-1683239 Msa0710070:CDS 45.0%
!! GTATATGGGCTTGGTTTGGA+TGG + chr5_1:1683229-1683248 Msa0710070:CDS 45.0%
TGGATTTAGACGTTGAGCCA+CGG - chr5_1:1683267-1683286 None:intergenic 45.0%
GATATTGCACGTGGTATGAG+TGG + chr5_1:1683307-1683326 Msa0710070:CDS 45.0%
CGTGGAAGCAATGCAAATGT+TGG + chr5_1:1683496-1683515 Msa0710070:CDS 45.0%
!! TGCAAATGTTGGTGCTGATG+TGG + chr5_1:1683507-1683526 Msa0710070:CDS 45.0%
AAAGGTTTGTGACCCCTTCC+TGG - chr5_1:1682021-1682040 None:intergenic 50.0%
GTCTCAACCTCATCCTTTGG+AGG + chr5_1:1683114-1683133 Msa0710070:CDS 50.0%
TTACCAGCCTCCAAAGGATG+AGG - chr5_1:1683124-1683143 None:intergenic 50.0%
! GAGGCTGGTAATGATGAGAG+TGG + chr5_1:1683133-1683152 Msa0710070:CDS 50.0%
GGATTTAGACGTTGAGCCAC+GGG - chr5_1:1683266-1683285 None:intergenic 50.0%
TGGTATGAGTGGAGAGGTTG+AGG + chr5_1:1683318-1683337 Msa0710070:CDS 50.0%
GGAGGTTCATTGCACACCCA+AGG + chr5_1:1681822-1681841 Msa0710070:CDS 55.0%
! CAACCTCATCCTTTGGAGGC+TGG + chr5_1:1683118-1683137 Msa0710070:CDS 55.0%
TGCACGTGGTATGAGTGGAG+AGG + chr5_1:1683312-1683331 Msa0710070:CDS 55.0%
GAGAGGTTGAGGCGCGTTTA+AGG + chr5_1:1683329-1683348 Msa0710070:CDS 55.0%
GATGGCAGCATGCGCACCCG+TGG + chr5_1:1683247-1683266 Msa0710070:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 1680393 1683778 1680393 ID=Msa0710070;Name=Msa0710070
chr5_1 mRNA 1680393 1683778 1680393 ID=Msa0710070-mRNA-1;Parent=Msa0710070;Name=Msa0710070-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|1|0.8|1|0|0.4|5|241|262
chr5_1 exon 1680393 1680409 1680393 ID=Msa0710070-mRNA-1:exon:555;Parent=Msa0710070-mRNA-1
chr5_1 exon 1680434 1680553 1680434 ID=Msa0710070-mRNA-1:exon:556;Parent=Msa0710070-mRNA-1
chr5_1 exon 1681788 1681878 1681788 ID=Msa0710070-mRNA-1:exon:557;Parent=Msa0710070-mRNA-1
chr5_1 exon 1681963 1682067 1681963 ID=Msa0710070-mRNA-1:exon:558;Parent=Msa0710070-mRNA-1
chr5_1 exon 1683082 1683778 1683082 ID=Msa0710070-mRNA-1:exon:559;Parent=Msa0710070-mRNA-1
chr5_1 CDS 1680393 1680409 1680393 ID=Msa0710070-mRNA-1:cds;Parent=Msa0710070-mRNA-1
chr5_1 CDS 1680434 1680553 1680434 ID=Msa0710070-mRNA-1:cds;Parent=Msa0710070-mRNA-1
chr5_1 CDS 1681788 1681878 1681788 ID=Msa0710070-mRNA-1:cds;Parent=Msa0710070-mRNA-1
chr5_1 CDS 1681963 1682067 1681963 ID=Msa0710070-mRNA-1:cds;Parent=Msa0710070-mRNA-1
chr5_1 CDS 1683082 1683537 1683082 ID=Msa0710070-mRNA-1:cds;Parent=Msa0710070-mRNA-1
chr5_1 three_prime_UTR 1683538 1683778 1683538 ID=Msa0710070-mRNA-1:three_prime_utr;Parent=Msa0710070-mRNA-1
Gene Sequence

>Msa0710070

TCTCACTCGTTCAATCGCTCTCTCGTTCAATCGCTCACTCGTTCAATCGCTCTCTCGTTCAATCGCTCACTCGTTCAATCGCTCTCAGCTTCTCAGTCTCGTAATCAGTTCAACTTCACAGTTCTTTGCAATTTCAGATGTGTGGCAGCTAGTAATCGTGATTCAGATGTCGGAGGTTCATTGCACACCCAAGGATCTAAGAATCAACTAAAGCATGAACTTGAATTGAACAAAAGGTTAGGAAGAATTCCACACAAGGGAGAGCTATTTAAAAAATGTCACACCAGGAAGGGGTCACAAACCTTTGTTGATAAGAGATCAACTGATGCATATAAAAACTTTGTAAATCTCTGTTCACAAGCTGAGTCTCAACCTCATCCTTTGGAGGCTGGTAATGATGAGAGTGGTACTCAACCCAAGCCTATTGATTATGATACACTAATGATTGAAGCTGTTGGAGGAAGAAATGCAAAGGGTAGAGTATATGGGCTTGGTTTGGATGGCAGCATGCGCACCCGTGGCTCAACGTCTAAATCCAATAAATATTCAAATTATACTGATATTGCACGTGGTATGAGTGGAGAGGTTGAGGCGCGTTTAAGGCAAAAAATTGCAGAGCAAAAACAAACAATCGATTCCATTAAGGCTCGTCAAAACAAAATGATTGCCGTATTTGCTAAACATGCAAATATAGATTTGGATTTAATGGATTTAGATGAAGATGATCACTCTGTTGATAATGCTACTCGTGGAAGCAATGCAAATGTTGGTGCTGATGTGGAAGAATAA
Protein sequence

>Msa0710070

SHSFNRSLVQSLTRSIALSFNRSLVQSLSASQSRNQFNFTVLCNFRCVAASNRDSDVGGSLHTQGSKNQLKHELELNKRLGRIPHKGELFKKCHTRKGSQTFVDKRSTDAYKNFVNLCSQAESQPHPLEAGNDESGTQPKPIDYDTLMIEAVGGRNAKGRVYGLGLDGSMRTRGSTSKSNKYSNYTDIARGMSGEVEARLRQKIAEQKQTIDSIKARQNKMIAVFAKHANIDLDLMDLDEDDHSVDNATRGSNANVGADVEE*