AlfalfaGEDB | Msa0740060

M. sativa cultivar zhongmu-4 / Msa0740060

KEGG: K09842|K11817|K22417
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004029 GO:0004031 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009687 GO:0009688 GO:0009850 GO:0009851 GO:0009987 GO:0010293 GO:0010817 GO:0016053 GO:0016106 GO:0016114 GO:0016143 GO:0016491 GO:0016620 GO:0016623 GO:0016903 GO:0018479 GO:0018488 GO:0019748 GO:0019752 GO:0019757 GO:0019760 GO:0019899 GO:0031625 GO:0032787 GO:0036094 GO:0042445 GO:0042446 GO:0043167 GO:0043168 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046394 GO:0048037 GO:0050302 GO:0050660 GO:0050662 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0097159 GO:1901135 GO:1901265 GO:1901363 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901657 GO:1902644 GO:1902645
PFAM: PF00111.30

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0740060	KEH28334.1	94.669	619	32	1	1	619	1	618	0.0	1139

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0740060	sp\|Q7G192\|ALDO2_ARATH	53.485	617	229	16	11	619	2	568	0.0	609

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0740060	G7KHG0	94.669	619	32	1	1	619	1	618	0.0	1139

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0094600	Msa0740060	-0.815838	7.530977e-52	-8.615850e-47
Msa0740060	Msa1109820	0.804762	1.848938e-49	-8.615850e-47
Msa0740060	Msa1152950	0.808259	3.380919e-50	-8.615850e-47
Msa0740060	Msa1174310	0.800196	1.614152e-48	-8.615850e-47
Msa0740060	Msa1235400	0.807494	4.919049e-50	-8.615850e-47
Msa0452810	Msa0740060	-0.823678	1.217139e-53	-8.615850e-47
Msa0585650	Msa0740060	-0.803593	3.237276e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0740060	MtrunA17_Chr5g0440261	94.669	619	32	1	1	619	1	618	0.0	1139
Msa0740060	MtrunA17_Chr5g0440251	76.367	622	141	5	1	619	1	619	0.0	948
Msa0740060	MtrunA17_Chr8g0337481	64.356	202	68	2	596	797	88	285	1.85e-82	265
Msa0740060	MtrunA17_Chr8g0337361	63.861	202	69	2	596	797	205	402	7.04e-81	265
Msa0740060	MtrunA17_Chr8g0337471	62.871	202	71	2	596	797	147	344	3.00e-80	261
Msa0740060	MtrunA17_Chr8g0337491	63.500	200	69	2	596	795	158	353	2.51e-79	261
Msa0740060	MtrunA17_Chr5g0445921	92.000	125	10	0	670	794	11	135	2.68e-77	245
Msa0740060	MtrunA17_Chr8g0337371	57.709	227	86	3	579	797	613	837	3.83e-76	263
Msa0740060	MtrunA17_Chr8g0337371	62.245	196	70	2	596	791	193	384	1.79e-72	253
Msa0740060	MtrunA17_Chr8g0337451	59.901	202	77	2	596	797	147	344	2.98e-75	248
Msa0740060	MtrunA17_Chr4g0043061	58.621	203	80	2	595	797	152	350	2.87e-74	245
Msa0740060	MtrunA17_Chr7g0214831	62.500	192	70	1	606	797	157	346	8.49e-74	244
Msa0740060	MtrunA17_Chr2g0307121	45.417	240	119	3	568	797	109	346	2.29e-58	202
Msa0740060	MtrunA17_Chr3g0127971	62.914	151	31	3	52	202	9	134	2.99e-55	187
Msa0740060	MtrunA17_Chr2g0322531	36.296	270	163	4	532	794	80	347	3.58e-44	163
Msa0740060	MtrunA17_Chr2g0322551	45.963	161	85	1	614	772	166	326	6.98e-42	156
Msa0740060	MtrunA17_Chr2g0322541	39.462	223	126	4	556	772	107	326	1.36e-39	151
Msa0740060	MtrunA17_Chr2g0328851	26.887	636	387	21	1	593	7	607	1.70e-33	139
Msa0740060	MtrunA17_Chr4g0045041	38.172	186	107	5	615	794	439	622	8.71e-24	107
Msa0740060	MtrunA17_Chr4g0045061	37.634	186	108	5	615	794	439	622	3.22e-23	105
Msa0740060	MtrunA17_Chr8g0335881	64.935	77	23	2	596	672	146	218	1.12e-20	91.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0740060	AT3G43600.1	53.485	617	229	16	11	619	2	568	0.0	609
Msa0740060	AT5G20960.1	52.989	619	260	14	8	619	17	611	0.0	608
Msa0740060	AT5G20960.2	52.989	619	260	14	8	619	17	611	0.0	608
Msa0740060	AT1G04580.1	52.188	617	245	12	12	619	6	581	0.0	601
Msa0740060	AT2G27150.1	54.010	611	244	12	12	619	3	579	0.0	585
Msa0740060	AT2G27150.2	54.010	611	244	12	12	619	3	579	0.0	585
Msa0740060	AT2G27150.4	54.010	611	244	12	12	619	3	579	0.0	584
Msa0740060	AT2G27150.5	49.095	497	216	12	126	619	1	463	8.51e-134	424
Msa0740060	AT2G27150.3	49.095	497	216	12	126	619	1	463	1.52e-132	424
Msa0740060	AT2G27150.6	49.095	497	216	12	126	619	1	463	1.52e-132	424
Msa0740060	AT1G26320.1	65.625	192	64	1	606	797	162	351	6.19e-80	261
Msa0740060	AT5G37980.1	64.583	192	66	1	606	797	164	353	1.89e-77	254
Msa0740060	AT5G17000.1	63.542	192	68	1	606	797	156	345	5.68e-77	253
Msa0740060	AT5G16970.1	64.062	192	67	1	606	797	156	345	7.92e-77	252
Msa0740060	AT5G16990.1	63.021	192	69	1	606	797	154	343	2.39e-76	251
Msa0740060	AT5G16980.1	62.500	192	70	1	606	797	50	239	4.33e-76	246
Msa0740060	AT5G16980.3	62.500	192	70	1	606	797	50	239	4.33e-76	246
Msa0740060	AT5G16980.2	51.837	245	108	2	561	797	63	305	4.86e-76	249
Msa0740060	AT1G26320.2	64.062	192	62	2	606	797	162	346	5.20e-76	250
Msa0740060	AT3G03080.1	61.979	192	71	1	606	797	161	350	2.26e-75	249
Msa0740060	AT5G16960.2	55.605	223	93	2	579	795	122	344	2.83e-75	248
Msa0740060	AT5G16960.1	55.605	223	93	2	579	795	122	344	2.83e-75	248
Msa0740060	AT5G37940.1	62.500	192	70	1	606	797	164	353	3.11e-74	246
Msa0740060	AT5G38000.2	60.733	191	73	1	605	795	144	332	4.10e-72	239
Msa0740060	AT5G38000.1	61.053	190	72	1	606	795	164	351	1.72e-71	238
Msa0740060	AT3G59845.2	60.440	182	72	0	614	795	63	244	6.48e-70	230
Msa0740060	AT3G59845.1	60.440	182	72	0	614	795	165	346	5.22e-69	231
Msa0740060	AT1G65560.2	57.547	212	82	3	592	797	65	274	5.86e-69	229
Msa0740060	AT1G65560.1	57.547	212	82	3	592	797	141	350	1.34e-67	228
Msa0740060	AT5G38000.3	59.043	188	74	2	606	793	164	348	1.87e-66	225
Msa0740060	AT4G34900.3	27.496	571	337	17	13	544	10	542	5.21e-37	150
Msa0740060	AT4G34900.1	27.113	568	343	16	13	544	10	542	6.22e-37	150
Msa0740060	AT4G34890.1	27.526	574	339	17	10	544	15	550	1.45e-36	149
Msa0740060	AT4G34900.2	24.898	490	298	15	91	544	3	458	4.92e-30	128
Msa0740060	AT5G17000.2	69.118	68	19	1	606	673	156	221	3.51e-21	94.0
Msa0740060	AT1G49670.1	35.385	195	116	6	606	794	428	618	1.36e-19	94.4
Msa0740060	AT1G49670.2	35.385	195	116	6	606	794	451	641	1.49e-19	94.0
Msa0740060	AT5G37960.1	45.783	83	44	1	716	797	25	107	5.66e-15	72.0

Find 146 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTCGGTTTGAACTCTTTC+TGG	0.085297	5_1:-58883982	Msa0740060:CDS
TTTGAACTCTTTCTGGAAAA+AGG	0.162836	5_1:-58883975	Msa0740060:CDS
ATAACCAATGGTTATTTAAA+TGG	0.170095	5_1:-58882888	Msa0740060:CDS
TTGTTCCTCCTTGTAATTAA+AGG	0.220154	5_1:+58882227	None:intergenic
GTCGAGCAATGGATCATATT+TGG	0.262670	5_1:+58884363	None:intergenic
CACGTCCATACTTCACTTAC+TGG	0.272957	5_1:+58885252	None:intergenic
AAATGCTGCTTTCCTTGTTC+AGG	0.290746	5_1:-58883189	Msa0740060:CDS
TGCTTCAAAGACTTGCTTCC+CGG	0.291533	5_1:+58882776	None:intergenic
AAAGTTGTTGTTAATAATAC+TGG	0.296448	5_1:-58883755	Msa0740060:CDS
GATACTTGTAGGTTGTCTTT+TGG	0.300306	5_1:-58883131	Msa0740060:CDS
GATCAATCAGGGATTGAAAT+TGG	0.303252	5_1:-58883650	Msa0740060:CDS
TAACCATTGGTTATCCTTGC+AGG	0.315380	5_1:+58882897	None:intergenic
TCAATTCATGAAAGATTTGC+AGG	0.318071	5_1:-58884232	Msa0740060:CDS
AGAGGAGCTTCAAAGTTTAT+TGG	0.341442	5_1:-58883807	Msa0740060:CDS
CATGTTGAGAAGTTCTTATC+AGG	0.342829	5_1:-58883074	Msa0740060:CDS
TCAGAAGCTATAAACAAATC+AGG	0.351547	5_1:+58883860	None:intergenic
GGAATGTCTGTTTCCCTCTT+CGG	0.369549	5_1:-58884175	Msa0740060:CDS
GGTGCTGCGCTACAAGCTTC+AGG	0.373464	5_1:-58882711	Msa0740060:intron
AAGTATATGTCTATTCCTTC+AGG	0.376687	5_1:+58881821	None:intergenic
CAAATCTTTCATGAATTGAA+TGG	0.381723	5_1:+58884236	None:intergenic
ACTTCTTGCTGTGGATTCAA+TGG	0.388300	5_1:-58883423	Msa0740060:CDS
GAAAACCAGTAAGTGAAGTA+TGG	0.402532	5_1:-58885257	Msa0740060:exon
AAGTGCTGGAAGTAAATATA+AGG	0.404456	5_1:-58882386	Msa0740060:intron
TGTTGTTAATAATACTGGTA+TGG	0.413382	5_1:-58883750	Msa0740060:CDS
TACTCATTTGCCTTCGGCCG+AGG	0.414638	5_1:-58882862	Msa0740060:CDS
TTATCTTTGTTTCATTGGCA+TGG	0.415973	5_1:+58883777	None:intergenic
AAACCATGATAGATACTTGT+AGG	0.416337	5_1:-58883142	Msa0740060:CDS
ACTCTATACAAGCGAATCTT+CGG	0.416763	5_1:-58883288	Msa0740060:CDS
GGCGAGCCAGTCACAAAATC+TGG	0.417282	5_1:-58882732	Msa0740060:CDS
AAAATCAAAAGGGATCAATC+AGG	0.418309	5_1:-58883662	Msa0740060:CDS
TGAAGCTCCTCTAAACTAGT+AGG	0.424239	5_1:+58883818	None:intergenic
GAATAAACCTACTAGTTTAG+AGG	0.426584	5_1:-58883825	Msa0740060:CDS
GTTTGAATGGCTTGCATTGG+AGG	0.429814	5_1:-58883378	Msa0740060:CDS
ACTTCTTCAATGTTCCATCT+AGG	0.430843	5_1:+58882202	None:intergenic
GACATATACTTTGACAATGT+TGG	0.436284	5_1:-58881809	Msa0740060:CDS
GAGACACCAGGTGGTGGCTC+AGG	0.439363	5_1:+58884121	None:intergenic
TGTTATGTTGTTGGAAGTGC+TGG	0.440024	5_1:-58882400	Msa0740060:CDS
GAAGAGGGAAACAGACATTC+CGG	0.440400	5_1:+58884177	None:intergenic
AGTGGTTGACGGATCAACTT+TGG	0.448442	5_1:+58885168	None:intergenic
TTTCCTACTTTATTGTCGTC+CGG	0.452444	5_1:-58882795	Msa0740060:CDS
GGCCTCATCAAACCCAAACT+TGG	0.459549	5_1:+58882248	None:intergenic
CGCGGTCGAATTGTGGTGGC+TGG	0.464338	5_1:-58881746	Msa0740060:CDS
GGCTAGTGTAGGTGGCAATC+TGG	0.464931	5_1:-58883489	Msa0740060:CDS
GATAAGTATATTGATATAAG+TGG	0.465422	5_1:-58883701	Msa0740060:CDS
TGGTTGCTTGCAGGTGGTTG+CGG	0.466386	5_1:-58884406	Msa0740060:intron
TGGTCAACTAGCAAAATTGA+TGG	0.468450	5_1:-58882425	Msa0740060:CDS
TAGCTTCTGAGAAACATAGT+TGG	0.471882	5_1:-58883847	Msa0740060:CDS
TGGATATACTCATTTGCCTT+CGG	0.473423	5_1:-58882868	Msa0740060:CDS
TTGATGGGATGTTATGTTGT+TGG	0.475372	5_1:-58882409	Msa0740060:CDS
CTTGTATAGAGTTCGATACT+AGG	0.476862	5_1:+58883299	None:intergenic
TTTGTGACTGGCTCGCCAAT+CGG	0.477819	5_1:+58882738	None:intergenic
AAGGAGGAACAAGACCTAGA+TGG	0.479388	5_1:-58882216	Msa0740060:CDS
ATCTTCCCTTCCCTAACATA+AGG	0.481674	5_1:+58881584	None:intergenic
TTATCTTATATTTAGAAATG+TGG	0.487276	5_1:+58885347	None:intergenic
CGCGGCGTCTCATGTTAAGA+AGG	0.487677	5_1:+58881765	None:intergenic
GCAAGTTGATAGCTTCATAC+AGG	0.487704	5_1:+58883030	None:intergenic
TGTTCAGGTGTCTCCTTGCA+AGG	0.493361	5_1:-58883174	Msa0740060:CDS
GTTGTTAATAATACTGGTAT+GGG	0.493709	5_1:-58883749	Msa0740060:CDS
GCAAAAGAATAAGTTTCCTT+CGG	0.494321	5_1:-58883459	Msa0740060:CDS
CGTGCTTCTCCTCGACCGCT+TGG	0.495712	5_1:-58883230	Msa0740060:CDS
AACATTGCTTCTAATGCAGC+TGG	0.496232	5_1:+58881521	None:intergenic
GGGAATCTTTGTCGCTGCAC+TGG	0.499285	5_1:-58884061	Msa0740060:CDS
TGATGAGGCCTTTAATTACA+AGG	0.501845	5_1:-58882235	Msa0740060:CDS
TATGTTGAAGATATAGCTAA+AGG	0.505580	5_1:-58881557	Msa0740060:CDS
AGCGCGTTTCCAAGCGGTCG+AGG	0.505779	5_1:+58883221	None:intergenic
AACTCAAGCAAAGTGGTTGA+CGG	0.509030	5_1:+58885157	None:intergenic
AAGAGGGAAACAGACATTCC+GGG	0.511760	5_1:+58884178	None:intergenic
TTCCTACTTTATTGTCGTCC+GGG	0.512455	5_1:-58882794	Msa0740060:CDS
TCCTCAACTCTGTCGAGCAA+TGG	0.513023	5_1:+58884352	None:intergenic
GGCATTGGAAACAGCAAACA+AGG	0.513482	5_1:-58884262	Msa0740060:CDS
ATATCCATTTAAATAACCAT+TGG	0.515425	5_1:+58882884	None:intergenic
GTTAAACTCGGTTGTGGTGA+AGG	0.515849	5_1:-58885106	Msa0740060:intron
ATGTTGAAGATATAGCTAAA+GGG	0.517100	5_1:-58881556	Msa0740060:CDS
ACACTACTTTGTAGCATACA+TGG	0.519645	5_1:-58884307	Msa0740060:CDS
TGACTGGTGCAAAGTTTGAA+TGG	0.530477	5_1:-58883391	Msa0740060:CDS
CAATGCGGTTTCTGTACTCC+CGG	0.532666	5_1:-58884196	Msa0740060:CDS
CTCGCCAATCGGATTATACT+CGG	0.534737	5_1:+58882749	None:intergenic
GGGAAGCAAGTCTTTGAAGC+AGG	0.538409	5_1:-58882774	Msa0740060:CDS
ATGTTCACAGGCAAAAGTGC+TGG	0.538532	5_1:-58881503	Msa0740060:CDS
AAGAGTGTTAAACTCGGTTG+TGG	0.542468	5_1:-58885112	Msa0740060:CDS
ATGTCGGAAGCCTCGGCCGA+AGG	0.549223	5_1:+58882852	None:intergenic
TTTCTTGAGAGACCACCGTT+AGG	0.556346	5_1:-58883353	Msa0740060:CDS
GATGTTGATATGGAGGATCT+CGG	0.561827	5_1:-58883998	Msa0740060:CDS
AAAGTTTGAATGGCTTGCAT+TGG	0.563510	5_1:-58883381	Msa0740060:CDS
AATTCTCCTGAGCCACCACC+TGG	0.563652	5_1:-58884127	Msa0740060:CDS
AGAAACCGCATTGCGAAGCA+TGG	0.564320	5_1:+58884206	None:intergenic
TCTTATATTTAGAAATGTGG+TGG	0.564334	5_1:+58885350	None:intergenic
ATAACAACAAGTGAAGGCAT+TGG	0.565209	5_1:-58884277	Msa0740060:CDS
GTTGATAGGTACTTTCCTGA+AGG	0.565387	5_1:-58881836	Msa0740060:intron
GAGACGCCGCGGTCGAATTG+TGG	0.565480	5_1:-58881753	Msa0740060:CDS
CAACCTACAAGTATCTATCA+TGG	0.566537	5_1:+58883139	None:intergenic
TCCATTGCTCGACAGAGTTG+AGG	0.567797	5_1:-58884353	Msa0740060:CDS
GACCAAGTTTGGGTTTGATG+AGG	0.568115	5_1:-58882250	Msa0740060:CDS
TACCTTTCGAGTTTAGCAGT+TGG	0.568761	5_1:-58882957	Msa0740060:CDS
CATAAAAGAGAATCAGAAGC+AGG	0.569744	5_1:-58882832	Msa0740060:CDS
AGTTACTATACTTCTTGCTG+TGG	0.573276	5_1:-58883432	Msa0740060:CDS
TGCAGCTGATGTTGATATGG+AGG	0.573289	5_1:-58884005	Msa0740060:CDS
ATATACTTTGACAATGTTGG+TGG	0.576525	5_1:-58881806	Msa0740060:CDS
TGCAGGCATCAGCAATCGGT+CGG	0.577811	5_1:+58884035	None:intergenic
CATATGAGCAAAGTTGCGAC+GGG	0.580291	5_1:-58883527	Msa0740060:CDS
GCATTAGAAGCAATGTTCAC+AGG	0.584098	5_1:-58881515	Msa0740060:CDS
TCAATGGTTCACATAATGAC+TGG	0.584244	5_1:-58883407	Msa0740060:CDS
AAATCAAAAGGGATCAATCA+GGG	0.587834	5_1:-58883661	Msa0740060:CDS
ATTCGGAACACGGCTAGTGT+AGG	0.592880	5_1:-58883500	Msa0740060:CDS
TAGCTAAAGGGATTGAGAGT+GGG	0.592897	5_1:-58881544	Msa0740060:CDS
TGTAGGTGGCAATCTGGTGA+TGG	0.594297	5_1:-58883483	Msa0740060:CDS
CGGAACACGGCTAGTGTAGG+TGG	0.595009	5_1:-58883497	Msa0740060:CDS
TCATATGAGCAAAGTTGCGA+CGG	0.595095	5_1:-58883528	Msa0740060:CDS
GTTTCATGAGTATCCTTGCA+AGG	0.598681	5_1:+58883161	None:intergenic
AACCAACTGCTAAACTCGAA+AGG	0.600066	5_1:+58882955	None:intergenic
TGTGTCAAGGTTTCATCTTG+AGG	0.600333	5_1:+58882984	None:intergenic
CAAGATGAAACCTTGACACA+TGG	0.603764	5_1:-58882981	Msa0740060:CDS
CGGTCGAGGAGAAGCACGGT+AGG	0.604039	5_1:+58883235	None:intergenic
GGCACCGAGTATAATCCGAT+TGG	0.607478	5_1:-58882753	Msa0740060:CDS
TGCAAGGAGACACCTGAACA+AGG	0.609368	5_1:+58883177	None:intergenic
TGTTCAATAACAACAAGTGA+AGG	0.612214	5_1:-58884283	Msa0740060:CDS
GTTGACAAGAGCACCGAAGA+GGG	0.613007	5_1:+58884162	None:intergenic
AGTATAGTAACTATATCCGA+AGG	0.623016	5_1:+58883443	None:intergenic
TAAGAAAGCGCGTTTCCAAG+CGG	0.625443	5_1:+58883215	None:intergenic
CACAGATTGAAAGTCCTGCA+AGG	0.628076	5_1:-58882911	Msa0740060:CDS
TTCCCGGACGACAATAAAGT+AGG	0.631349	5_1:+58882792	None:intergenic
CGAAAGGTAACCATGTGTCA+AGG	0.633047	5_1:+58882971	None:intergenic
AGGGATTGAAATTGGAGCAG+CGG	0.634981	5_1:-58883642	Msa0740060:CDS
GCGGCGTCTCATGTTAAGAA+GGG	0.635176	5_1:+58881766	None:intergenic
TATGAACTTGATGAACCTCA+AGG	0.637072	5_1:-58881710	Msa0740060:CDS
CAATTCAAGAGTGTTAAACT+CGG	0.637477	5_1:-58885118	Msa0740060:CDS
TTGTGATCACGATCGTATCG+AGG	0.638841	5_1:+58883920	None:intergenic
ACGTAAGAACTCAAGCAAAG+TGG	0.639962	5_1:+58885150	None:intergenic
AGTCCTGCAAGGATAACCAA+TGG	0.640489	5_1:-58882900	Msa0740060:CDS
TAGTGTGAGGCAAGAGCTCG+CGG	0.644034	5_1:+58884324	None:intergenic
GGATTCCATGCTTCGCAATG+CGG	0.645234	5_1:-58884211	Msa0740060:CDS
ATAGCTAAAGGGATTGAGAG+TGG	0.645427	5_1:-58881545	Msa0740060:CDS
GGTCAACTAGCAAAATTGAT+GGG	0.646505	5_1:-58882424	Msa0740060:CDS
AGAACACTTTCAAAACCTAA+CGG	0.648349	5_1:+58883338	None:intergenic
CGTTGACAAGAGCACCGAAG+AGG	0.648990	5_1:+58884161	None:intergenic
ACGCCGCGGTCGAATTGTGG+TGG	0.652143	5_1:-58881750	Msa0740060:CDS
CAAGCGGTCGAGGAGAAGCA+CGG	0.658870	5_1:+58883231	None:intergenic
TATTTAGAAATGTGGTGGTG+AGG	0.660703	5_1:+58885355	None:intergenic
TTCATCTTGAGGTGTAATAG+CGG	0.666785	5_1:+58882995	None:intergenic
GGATATTACAAAGATAAACA+AGG	0.678422	5_1:-58883728	Msa0740060:CDS
GTATGCTACAAAGTAGTGTG+AGG	0.688383	5_1:+58884311	None:intergenic
TGAGGCCTTTAATTACAAGG+AGG	0.704335	5_1:-58882232	Msa0740060:CDS
AAGCACGGTAGGTTTCAAAG+AGG	0.726582	5_1:+58883246	None:intergenic
ACACTTTCAAAACCTAACGG+TGG	0.726815	5_1:+58883341	None:intergenic
CTTCTTAACATGAGACGCCG+CGG	0.740483	5_1:-58881764	Msa0740060:CDS
CAGCCACCACAATTCGACCG+CGG	0.745630	5_1:+58881747	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATCTTATATTTAGAAATG+TGG	+	chr5_1:58881480-58881499	None:intergenic	15.0%
!!!	TCATATATATTGAATTTTTC+TGG	-	chr5_1:58881749-58881768	Msa0740060:CDS	15.0%
!!	AAAATTATTGATATAGTTGA+AGG	+	chr5_1:58882095-58882114	None:intergenic	15.0%
!!	ATGTAAAAGTAATAATTAAC+AGG	+	chr5_1:58884181-58884200	None:intergenic	15.0%
!!	AAAACAAAACAATATCAATT+AGG	+	chr5_1:58884524-58884543	None:intergenic	15.0%
!!	ATTACTCTAATCATAGTTTA+AGG	-	chr5_1:58882026-58882045	Msa0740060:intron	20.0%
!!	CAAATTTGAAAAGAAAATTG+AGG	+	chr5_1:58882380-58882399	None:intergenic	20.0%
!!!	CTCAATTTTCTTTTCAAATT+TGG	-	chr5_1:58882378-58882397	Msa0740060:intron	20.0%
!!!	AACTTTTATCTTTGTTTCAT+TGG	+	chr5_1:58883055-58883074	None:intergenic	20.0%
!!!	AAAGTTGTTGTTAATAATAC+TGG	-	chr5_1:58883069-58883088	Msa0740060:CDS	20.0%
!!	GATAAGTATATTGATATAAG+TGG	-	chr5_1:58883123-58883142	Msa0740060:CDS	20.0%
!!	ATAACCAATGGTTATTTAAA+TGG	-	chr5_1:58883936-58883955	Msa0740060:CDS	20.0%
!!	ATATCCATTTAAATAACCAT+TGG	+	chr5_1:58883943-58883962	None:intergenic	20.0%
!!	TTTCTGAATAAATCACATAT+AGG	-	chr5_1:58884239-58884258	Msa0740060:CDS	20.0%
!!!	TGTTTTCATGTCAATAAATA+AGG	-	chr5_1:58884538-58884557	Msa0740060:intron	20.0%
!!	ACTTGTATACAAAAATTAAC+AGG	-	chr5_1:58884685-58884704	Msa0740060:intron	20.0%
!!	CTTGTATACAAAAATTAACA+GGG	-	chr5_1:58884686-58884705	Msa0740060:intron	20.0%
!!	TAGAATACAATACTTACTTT+AGG	-	chr5_1:58884794-58884813	Msa0740060:intron	20.0%
!!	TTTAGTATTCATTACAAAAG+AGG	+	chr5_1:58884937-58884956	None:intergenic	20.0%
!	TCTTATATTTAGAAATGTGG+TGG	+	chr5_1:58881477-58881496	None:intergenic	25.0%
!	TCATACTGAAACATAAAGAA+AGG	+	chr5_1:58881915-58881934	None:intergenic	25.0%
!	CATGTATATAAACATTCCTT+AGG	+	chr5_1:58882052-58882071	None:intergenic	25.0%
!!	AATTCTTTTCTCAAATCTTG+TGG	+	chr5_1:58882160-58882179	None:intergenic	25.0%
!	ACAAGATTTGAGAAAAGAAT+TGG	-	chr5_1:58882159-58882178	Msa0740060:intron	25.0%
!!	GATATTTTACGAAAAACAAC+AGG	-	chr5_1:58882275-58882294	Msa0740060:intron	25.0%
!	AAATTTGGAAATTCATGCTT+TGG	-	chr5_1:58882393-58882412	Msa0740060:CDS	25.0%
!	CAAATCTTTCATGAATTGAA+TGG	+	chr5_1:58882591-58882610	None:intergenic	25.0%
!	TGTTGTTAATAATACTGGTA+TGG	-	chr5_1:58883074-58883093	Msa0740060:CDS	25.0%
!	GTTGTTAATAATACTGGTAT+GGG	-	chr5_1:58883075-58883094	Msa0740060:CDS	25.0%
!	GGATATTACAAAGATAAACA+AGG	-	chr5_1:58883096-58883115	Msa0740060:CDS	25.0%
!	TGAGCTTTCAAAAATCAAAA+GGG	-	chr5_1:58883152-58883171	Msa0740060:CDS	25.0%
!!!	ATATTTTGAAGACCAAGTTT+GGG	-	chr5_1:58884564-58884583	Msa0740060:intron	25.0%
!!!	ATTAAGTTTTTTATGCCTTG+AGG	+	chr5_1:58885132-58885151	None:intergenic	25.0%
!!	TCACTTATATCCAAAGTTTT+TGG	-	chr5_1:58885206-58885225	Msa0740060:CDS	25.0%
!	AAAATTGTGTCCAAAAACTT+TGG	+	chr5_1:58885219-58885238	None:intergenic	25.0%
!	TATGTTGAAGATATAGCTAA+AGG	-	chr5_1:58885267-58885286	Msa0740060:five_prime_UTR	25.0%
!	ATGTTGAAGATATAGCTAAA+GGG	-	chr5_1:58885268-58885287	Msa0740060:five_prime_UTR	25.0%
!!	AGTTTGATTTTCTGTGTCAA+TGG	-	chr5_1:58881616-58881635	Msa0740060:CDS	30.0%
!!	CAATTCAAGAGTGTTAAACT+CGG	-	chr5_1:58881706-58881725	Msa0740060:CDS	30.0%
!!!	TTGAATTTTTCTGGCTTCTT+TGG	-	chr5_1:58881758-58881777	Msa0740060:CDS	30.0%
!!	CTTTTTATGATTCTGAGAGT+TGG	-	chr5_1:58881946-58881965	Msa0740060:intron	30.0%
!	TAATCATAGTTTAAGGCCTA+AGG	-	chr5_1:58882033-58882052	Msa0740060:intron	30.0%
	GTTTATATACATGTCTGTAC+TGG	-	chr5_1:58882059-58882078	Msa0740060:intron	30.0%
	TGATATAGTTGAAGGATCAT+AGG	+	chr5_1:58882087-58882106	None:intergenic	30.0%
	TTTCGTAAAATATCTGCATC+TGG	+	chr5_1:58882269-58882288	None:intergenic	30.0%
	AACGATGTACATAAACTTCA+AGG	+	chr5_1:58882340-58882359	None:intergenic	30.0%
	TGTTCAATAACAACAAGTGA+AGG	-	chr5_1:58882541-58882560	Msa0740060:intron	30.0%
	TCAATTCATGAAAGATTTGC+AGG	-	chr5_1:58882592-58882611	Msa0740060:intron	30.0%
!	TTTGAACTCTTTCTGGAAAA+AGG	-	chr5_1:58882849-58882868	Msa0740060:CDS	30.0%
!	TTGAACTCTTTCTGGAAAAA+GGG	-	chr5_1:58882850-58882869	Msa0740060:CDS	30.0%
!!	CGAATTTCCTATGTTTTTGA+AGG	-	chr5_1:58882933-58882952	Msa0740060:CDS	30.0%
	TTGACTTCCTTCAAAAACAT+AGG	+	chr5_1:58882943-58882962	None:intergenic	30.0%
	TCAGAAGCTATAAACAAATC+AGG	+	chr5_1:58882967-58882986	None:intergenic	30.0%
!	GAATAAACCTACTAGTTTAG+AGG	-	chr5_1:58882999-58883018	Msa0740060:CDS	30.0%
!	TTATCTTTGTTTCATTGGCA+TGG	+	chr5_1:58883050-58883069	None:intergenic	30.0%
	CTGAGCTTTCAAAAATCAAA+AGG	-	chr5_1:58883151-58883170	Msa0740060:CDS	30.0%
	AAAATCAAAAGGGATCAATC+AGG	-	chr5_1:58883162-58883181	Msa0740060:CDS	30.0%
	AAATCAAAAGGGATCAATCA+GGG	-	chr5_1:58883163-58883182	Msa0740060:CDS	30.0%
	GCAAAAGAATAAGTTTCCTT+CGG	-	chr5_1:58883365-58883384	Msa0740060:CDS	30.0%
	AGTATAGTAACTATATCCGA+AGG	+	chr5_1:58883384-58883403	None:intergenic	30.0%
	AGAACACTTTCAAAACCTAA+CGG	+	chr5_1:58883489-58883508	None:intergenic	30.0%
	AAACCATGATAGATACTTGT+AGG	-	chr5_1:58883682-58883701	Msa0740060:CDS	30.0%
!	CTTCTGATTCTCTTTTATGT+CGG	+	chr5_1:58883991-58884010	None:intergenic	30.0%
!!	AAGTGCTGGAAGTAAATATA+AGG	-	chr5_1:58884438-58884457	Msa0740060:intron	30.0%
!!	GATATTTTGAAGACCAAGTT+TGG	-	chr5_1:58884563-58884582	Msa0740060:intron	30.0%
	TTGTTCCTCCTTGTAATTAA+AGG	+	chr5_1:58884600-58884619	None:intergenic	30.0%
	AAGCGAAAAGCTTTACTAAT+CGG	+	chr5_1:58884725-58884744	None:intergenic	30.0%
!	ATTAGTAAAGCTTTTCGCTT+AGG	-	chr5_1:58884725-58884744	Msa0740060:intron	30.0%
!	AGGTTCTTGATGATTAACAT+CGG	+	chr5_1:58884851-58884870	None:intergenic	30.0%
	TGTTAATCATCAAGAACCTA+AGG	-	chr5_1:58884852-58884871	Msa0740060:intron	30.0%
!	TGAGAAAAATGGATTTCCTT+AGG	+	chr5_1:58884871-58884890	None:intergenic	30.0%
	CTAATCTTAGCTGAGAAAAA+TGG	+	chr5_1:58884882-58884901	None:intergenic	30.0%
	GTTCTGTTTCAATTGTTGAT+AGG	-	chr5_1:58884974-58884993	Msa0740060:intron	30.0%
	AAGTATATGTCTATTCCTTC+AGG	+	chr5_1:58885006-58885025	None:intergenic	30.0%
	GACATATACTTTGACAATGT+TGG	-	chr5_1:58885015-58885034	Msa0740060:intron	30.0%
	ATATACTTTGACAATGTTGG+TGG	-	chr5_1:58885018-58885037	Msa0740060:intron	30.0%
!	ACACAATTTTGCCTTATGTT+AGG	-	chr5_1:58885229-58885248	Msa0740060:CDS	30.0%
!	CACAATTTTGCCTTATGTTA+GGG	-	chr5_1:58885230-58885249	Msa0740060:CDS	30.0%
	GAAAACCAGTAAGTGAAGTA+TGG	-	chr5_1:58881567-58881586	Msa0740060:CDS	35.0%
!!!	TTTTCTGGCTTCTTTGGATT+TGG	-	chr5_1:58881764-58881783	Msa0740060:CDS	35.0%
	ACACTACTTTGTAGCATACA+TGG	-	chr5_1:58882517-58882536	Msa0740060:intron	35.0%
!	ATAACAACAAGTGAAGGCAT+TGG	-	chr5_1:58882547-58882566	Msa0740060:intron	35.0%
!!	AGATTTTTCAGCCTTGGAAA+CGG	+	chr5_1:58882737-58882756	None:intergenic	35.0%
!!	TGCAATAGATTTTTCAGCCT+TGG	+	chr5_1:58882743-58882762	None:intergenic	35.0%
	AAGGCTGAAAAATCTATTGC+AGG	-	chr5_1:58882742-58882761	Msa0740060:CDS	35.0%
	AGGCTGAAAAATCTATTGCA+GGG	-	chr5_1:58882743-58882762	Msa0740060:CDS	35.0%
!	TTTTGCAGCTGATGTTGATA+TGG	-	chr5_1:58882816-58882835	Msa0740060:CDS	35.0%
!	TAGCTTCTGAGAAACATAGT+TGG	-	chr5_1:58882977-58882996	Msa0740060:CDS	35.0%
	AGAGGAGCTTCAAAGTTTAT+TGG	-	chr5_1:58883017-58883036	Msa0740060:CDS	35.0%
	GATCAATCAGGGATTGAAAT+TGG	-	chr5_1:58883174-58883193	Msa0740060:CDS	35.0%
	AGTTACTATACTTCTTGCTG+TGG	-	chr5_1:58883392-58883411	Msa0740060:CDS	35.0%
	TCAATGGTTCACATAATGAC+TGG	-	chr5_1:58883417-58883436	Msa0740060:CDS	35.0%
!	AAAGTTTGAATGGCTTGCAT+TGG	-	chr5_1:58883443-58883462	Msa0740060:CDS	35.0%
	CTTGTATAGAGTTCGATACT+AGG	+	chr5_1:58883528-58883547	None:intergenic	35.0%
	ACTCTATACAAGCGAATCTT+CGG	-	chr5_1:58883536-58883555	Msa0740060:CDS	35.0%
	CAACCTACAAGTATCTATCA+TGG	+	chr5_1:58883688-58883707	None:intergenic	35.0%
!	GATACTTGTAGGTTGTCTTT+TGG	-	chr5_1:58883693-58883712	Msa0740060:CDS	35.0%
	CATGTTGAGAAGTTCTTATC+AGG	-	chr5_1:58883750-58883769	Msa0740060:CDS	35.0%
	TTCATCTTGAGGTGTAATAG+CGG	+	chr5_1:58883832-58883851	None:intergenic	35.0%
	TGGATATACTCATTTGCCTT+CGG	-	chr5_1:58883956-58883975	Msa0740060:CDS	35.0%
	CATAAAAGAGAATCAGAAGC+AGG	-	chr5_1:58883992-58884011	Msa0740060:CDS	35.0%
	TTTCCTACTTTATTGTCGTC+CGG	-	chr5_1:58884029-58884048	Msa0740060:CDS	35.0%
	GAATAAATCACATATAGGCG+AGG	-	chr5_1:58884244-58884263	Msa0740060:CDS	35.0%
	TGGTCAACTAGCAAAATTGA+TGG	-	chr5_1:58884399-58884418	Msa0740060:intron	35.0%
	GGTCAACTAGCAAAATTGAT+GGG	-	chr5_1:58884400-58884419	Msa0740060:intron	35.0%
	TTGATGGGATGTTATGTTGT+TGG	-	chr5_1:58884415-58884434	Msa0740060:intron	35.0%
	TGATGAGGCCTTTAATTACA+AGG	-	chr5_1:58884589-58884608	Msa0740060:intron	35.0%
	ACTTCTTCAATGTTCCATCT+AGG	+	chr5_1:58884625-58884644	None:intergenic	35.0%
	AAATTAACAGGGCAATCCAA+AGG	-	chr5_1:58884697-58884716	Msa0740060:intron	35.0%
	AATTAACAGGGCAATCCAAA+GGG	-	chr5_1:58884698-58884717	Msa0740060:intron	35.0%
	TATGAACTTGATGAACCTCA+AGG	-	chr5_1:58885114-58885133	Msa0740060:CDS	35.0%
!	ATTTTGCCTTATGTTAGGGA+AGG	-	chr5_1:58885234-58885253	Msa0740060:CDS	35.0%
!	TTTTGCCTTATGTTAGGGAA+GGG	-	chr5_1:58885235-58885254	Msa0740060:CDS	35.0%
	AACTCAAGCAAAGTGGTTGA+CGG	+	chr5_1:58881670-58881689	None:intergenic	40.0%
	ACGTAAGAACTCAAGCAAAG+TGG	+	chr5_1:58881677-58881696	None:intergenic	40.0%
!!	AAGAGTGTTAAACTCGGTTG+TGG	-	chr5_1:58881712-58881731	Msa0740060:CDS	40.0%
	TGGAAATTCATGCTTTGGCA+TGG	-	chr5_1:58882398-58882417	Msa0740060:CDS	40.0%
	GTCGAGCAATGGATCATATT+TGG	+	chr5_1:58882464-58882483	None:intergenic	40.0%
	GTATGCTACAAAGTAGTGTG+AGG	+	chr5_1:58882516-58882535	None:intergenic	40.0%
	CTCAAAAATCACCGTTTCCA+AGG	-	chr5_1:58882723-58882742	Msa0740060:CDS	40.0%
!	ATCAGCTGCAAAACTTTTGC+AGG	+	chr5_1:58882809-58882828	None:intergenic	40.0%
	GATGTTGATATGGAGGATCT+CGG	-	chr5_1:58882826-58882845	Msa0740060:CDS	40.0%
!	ATCTCGGTTTGAACTCTTTC+TGG	-	chr5_1:58882842-58882861	Msa0740060:CDS	40.0%
	TGAAGCTCCTCTAAACTAGT+AGG	+	chr5_1:58883009-58883028	None:intergenic	40.0%
	TCATATGAGCAAAGTTGCGA+CGG	-	chr5_1:58883296-58883315	Msa0740060:CDS	40.0%
!!!	AAGTTGCGACGGGTTTTATT+CGG	-	chr5_1:58883307-58883326	Msa0740060:CDS	40.0%
!	ACTTCTTGCTGTGGATTCAA+TGG	-	chr5_1:58883401-58883420	Msa0740060:CDS	40.0%
	TGACTGGTGCAAAGTTTGAA+TGG	-	chr5_1:58883433-58883452	Msa0740060:CDS	40.0%
	ACACTTTCAAAACCTAACGG+TGG	+	chr5_1:58883486-58883505	None:intergenic	40.0%
!!	AAATGCTGCTTTCCTTGTTC+AGG	-	chr5_1:58883635-58883654	Msa0740060:CDS	40.0%
	GTTTCATGAGTATCCTTGCA+AGG	+	chr5_1:58883666-58883685	None:intergenic	40.0%
!	ACTTGTAGGTTGTCTTTTGG+TGG	-	chr5_1:58883696-58883715	Msa0740060:CDS	40.0%
!!	GGTTGTCTTTTGGTGGTTTT+AGG	-	chr5_1:58883703-58883722	Msa0740060:CDS	40.0%
!!	GCAAGTTGATAGCTTCATAC+AGG	+	chr5_1:58883797-58883816	None:intergenic	40.0%
	TGTGTCAAGGTTTCATCTTG+AGG	+	chr5_1:58883843-58883862	None:intergenic	40.0%
	CAAGATGAAACCTTGACACA+TGG	-	chr5_1:58883843-58883862	Msa0740060:CDS	40.0%
!!	TACCTTTCGAGTTTAGCAGT+TGG	-	chr5_1:58883867-58883886	Msa0740060:CDS	40.0%
	AACCAACTGCTAAACTCGAA+AGG	+	chr5_1:58883872-58883891	None:intergenic	40.0%
!	TAACCATTGGTTATCCTTGC+AGG	+	chr5_1:58883930-58883949	None:intergenic	40.0%
	TTCCTACTTTATTGTCGTCC+GGG	-	chr5_1:58884030-58884049	Msa0740060:CDS	40.0%
!	TGTTATGTTGTTGGAAGTGC+TGG	-	chr5_1:58884424-58884443	Msa0740060:intron	40.0%
	TGAGGCCTTTAATTACAAGG+AGG	-	chr5_1:58884592-58884611	Msa0740060:intron	40.0%
!!!	TTTAGGCCTCACTTTTTGCT+GGG	-	chr5_1:58884811-58884830	Msa0740060:intron	40.0%
!	GTTGATAGGTACTTTCCTGA+AGG	-	chr5_1:58884988-58885007	Msa0740060:intron	40.0%
	ATCTTCCCTTCCCTAACATA+AGG	+	chr5_1:58885243-58885262	None:intergenic	40.0%
	ATAGCTAAAGGGATTGAGAG+TGG	-	chr5_1:58885279-58885298	Msa0740060:five_prime_UTR	40.0%
	TAGCTAAAGGGATTGAGAGT+GGG	-	chr5_1:58885280-58885299	Msa0740060:five_prime_UTR	40.0%
	AACATTGCTTCTAATGCAGC+TGG	+	chr5_1:58885306-58885325	None:intergenic	40.0%
	GCATTAGAAGCAATGTTCAC+AGG	-	chr5_1:58885309-58885328	Msa0740060:five_prime_UTR	40.0%
	CACGTCCATACTTCACTTAC+TGG	+	chr5_1:58881575-58881594	None:intergenic	45.0%
!	AGTGGTTGACGGATCAACTT+TGG	+	chr5_1:58881659-58881678	None:intergenic	45.0%
	GTTAAACTCGGTTGTGGTGA+AGG	-	chr5_1:58881718-58881737	Msa0740060:CDS	45.0%
!	GGCATTGGAAACAGCAAACA+AGG	-	chr5_1:58882562-58882581	Msa0740060:intron	45.0%
	AAGAGGGAAACAGACATTCC+GGG	+	chr5_1:58882649-58882668	None:intergenic	45.0%
	GAAGAGGGAAACAGACATTC+CGG	+	chr5_1:58882650-58882669	None:intergenic	45.0%
	GGAATGTCTGTTTCCCTCTT+CGG	-	chr5_1:58882649-58882668	Msa0740060:intron	45.0%
!!!	ATTTTTGAGACACCAGGTGG+TGG	+	chr5_1:58882712-58882731	None:intergenic	45.0%
!!!	GTGATTTTTGAGACACCAGG+TGG	+	chr5_1:58882715-58882734	None:intergenic	45.0%
!!!	ACGGTGATTTTTGAGACACC+AGG	+	chr5_1:58882718-58882737	None:intergenic	45.0%
!	CTTTTGCAGGCATCAGCAAT+CGG	+	chr5_1:58882796-58882815	None:intergenic	45.0%
	TGCAGCTGATGTTGATATGG+AGG	-	chr5_1:58882819-58882838	Msa0740060:CDS	45.0%
	TTGTGATCACGATCGTATCG+AGG	+	chr5_1:58882907-58882926	None:intergenic	45.0%
	AGGGATTGAAATTGGAGCAG+CGG	-	chr5_1:58883182-58883201	Msa0740060:CDS	45.0%
	CATATGAGCAAAGTTGCGAC+GGG	-	chr5_1:58883297-58883316	Msa0740060:CDS	45.0%
!!!	GACGGGTTTTATTCGGAACA+CGG	-	chr5_1:58883314-58883333	Msa0740060:CDS	45.0%
!	GTTTGAATGGCTTGCATTGG+AGG	-	chr5_1:58883446-58883465	Msa0740060:CDS	45.0%
	TTTCTTGAGAGACCACCGTT+AGG	-	chr5_1:58883471-58883490	Msa0740060:CDS	45.0%
	AAGCACGGTAGGTTTCAAAG+AGG	+	chr5_1:58883581-58883600	None:intergenic	45.0%
!	TAAGAAAGCGCGTTTCCAAG+CGG	+	chr5_1:58883612-58883631	None:intergenic	45.0%
	CGAAAGGTAACCATGTGTCA+AGG	+	chr5_1:58883856-58883875	None:intergenic	45.0%
	CACAGATTGAAAGTCCTGCA+AGG	-	chr5_1:58883913-58883932	Msa0740060:CDS	45.0%
	AGTCCTGCAAGGATAACCAA+TGG	-	chr5_1:58883924-58883943	Msa0740060:CDS	45.0%
!	CTCTTTTATGTCGGAAGCCT+CGG	+	chr5_1:58883982-58884001	None:intergenic	45.0%
	TTCCCGGACGACAATAAAGT+AGG	+	chr5_1:58884035-58884054	None:intergenic	45.0%
!	TGCTTCAAAGACTTGCTTCC+CGG	+	chr5_1:58884051-58884070	None:intergenic	45.0%
	CTCGCCAATCGGATTATACT+CGG	+	chr5_1:58884078-58884097	None:intergenic	45.0%
	TATAGGCGAGGCTGTATATG+TGG	-	chr5_1:58884256-58884275	Msa0740060:CDS	45.0%
!	GACCAAGTTTGGGTTTGATG+AGG	-	chr5_1:58884574-58884593	Msa0740060:intron	45.0%
	AAGGAGGAACAAGACCTAGA+TGG	-	chr5_1:58884608-58884627	Msa0740060:intron	45.0%
	GCTTTACTAATCGGTCCCTT+TGG	+	chr5_1:58884716-58884735	None:intergenic	45.0%
!!!	CTTTAGGCCTCACTTTTTGC+TGG	-	chr5_1:58884810-58884829	Msa0740060:intron	45.0%
!	ATGTTCACAGGCAAAAGTGC+TGG	-	chr5_1:58885321-58885340	Msa0740060:five_prime_UTR	45.0%
	TCCATTGCTCGACAGAGTTG+AGG	-	chr5_1:58882471-58882490	Msa0740060:intron	50.0%
	TCCTCAACTCTGTCGAGCAA+TGG	+	chr5_1:58882475-58882494	None:intergenic	50.0%
	GGATTCCATGCTTCGCAATG+CGG	-	chr5_1:58882613-58882632	Msa0740060:intron	50.0%
	AGAAACCGCATTGCGAAGCA+TGG	+	chr5_1:58882621-58882640	None:intergenic	50.0%
	CAATGCGGTTTCTGTACTCC+CGG	-	chr5_1:58882628-58882647	Msa0740060:intron	50.0%
!!	GTTGACAAGAGCACCGAAGA+GGG	+	chr5_1:58882665-58882684	None:intergenic	50.0%
	ATTCGGAACACGGCTAGTGT+AGG	-	chr5_1:58883324-58883343	Msa0740060:CDS	50.0%
	TGTAGGTGGCAATCTGGTGA+TGG	-	chr5_1:58883341-58883360	Msa0740060:CDS	50.0%
	TGCAAGGAGACACCTGAACA+AGG	+	chr5_1:58883650-58883669	None:intergenic	50.0%
	TGTTCAGGTGTCTCCTTGCA+AGG	-	chr5_1:58883650-58883669	Msa0740060:CDS	50.0%
!!	GGGAAGCAAGTCTTTGAAGC+AGG	-	chr5_1:58884050-58884069	Msa0740060:CDS	50.0%
!	GGCACCGAGTATAATCCGAT+TGG	-	chr5_1:58884071-58884090	Msa0740060:CDS	50.0%
!!	TTTGTGACTGGCTCGCCAAT+CGG	+	chr5_1:58884089-58884108	None:intergenic	50.0%
!	GCAGCACCAGATTTTGTGAC+TGG	+	chr5_1:58884101-58884120	None:intergenic	50.0%
	GGTGCAGTTGGTCAACTTGT+TGG	-	chr5_1:58884379-58884398	Msa0740060:CDS	50.0%
	GGCCTCATCAAACCCAAACT+TGG	+	chr5_1:58884579-58884598	None:intergenic	50.0%
	CATCAGCCCAGCAAAAAGTG+AGG	+	chr5_1:58884820-58884839	None:intergenic	50.0%
	GCGGCGTCTCATGTTAAGAA+GGG	+	chr5_1:58885061-58885080	None:intergenic	50.0%
	CTTCTTAACATGAGACGCCG+CGG	-	chr5_1:58885060-58885079	Msa0740060:intron	50.0%
	GCTTTGGCATGGTTGCTTGC+AGG	-	chr5_1:58882409-58882428	Msa0740060:CDS	55.0%
	TTGGCATGGTTGCTTGCAGG+TGG	-	chr5_1:58882412-58882431	Msa0740060:CDS	55.0%
	TGGTTGCTTGCAGGTGGTTG+CGG	-	chr5_1:58882418-58882437	Msa0740060:CDS	55.0%
	TAGTGTGAGGCAAGAGCTCG+CGG	+	chr5_1:58882503-58882522	None:intergenic	55.0%
!!	CGTTGACAAGAGCACCGAAG+AGG	+	chr5_1:58882666-58882685	None:intergenic	55.0%
	AATTCTCCTGAGCCACCACC+TGG	-	chr5_1:58882697-58882716	Msa0740060:intron	55.0%
	GGGAATCTTTGTCGCTGCAC+TGG	-	chr5_1:58882763-58882782	Msa0740060:CDS	55.0%
	TGCAGGCATCAGCAATCGGT+CGG	+	chr5_1:58882792-58882811	None:intergenic	55.0%
	GGCTAGTGTAGGTGGCAATC+TGG	-	chr5_1:58883335-58883354	Msa0740060:CDS	55.0%
	TACTCATTTGCCTTCGGCCG+AGG	-	chr5_1:58883962-58883981	Msa0740060:CDS	55.0%
	GGCGAGCCAGTCACAAAATC+TGG	-	chr5_1:58884092-58884111	Msa0740060:CDS	55.0%
	CGCGGCGTCTCATGTTAAGA+AGG	+	chr5_1:58885062-58885081	None:intergenic	55.0%
	CGGAACACGGCTAGTGTAGG+TGG	-	chr5_1:58883327-58883346	Msa0740060:CDS	60.0%
	CAAGCGGTCGAGGAGAAGCA+CGG	+	chr5_1:58883596-58883615	None:intergenic	60.0%
!	GGTGCTGCGCTACAAGCTTC+AGG	-	chr5_1:58884113-58884132	Msa0740060:CDS	60.0%
	CAGCCACCACAATTCGACCG+CGG	+	chr5_1:58885080-58885099	None:intergenic	60.0%
!	GAGACACCAGGTGGTGGCTC+AGG	+	chr5_1:58882706-58882725	None:intergenic	65.0%
!	CGGTCGAGGAGAAGCACGGT+AGG	+	chr5_1:58883592-58883611	None:intergenic	65.0%
	CGTGCTTCTCCTCGACCGCT+TGG	-	chr5_1:58883594-58883613	Msa0740060:CDS	65.0%
	AGCGCGTTTCCAAGCGGTCG+AGG	+	chr5_1:58883606-58883625	None:intergenic	65.0%
	ATGTCGGAAGCCTCGGCCGA+AGG	+	chr5_1:58883975-58883994	None:intergenic	65.0%
	GAGACGCCGCGGTCGAATTG+TGG	-	chr5_1:58885071-58885090	Msa0740060:intron	65.0%
	ACGCCGCGGTCGAATTGTGG+TGG	-	chr5_1:58885074-58885093	Msa0740060:intron	65.0%
	CGCGGTCGAATTGTGGTGGC+TGG	-	chr5_1:58885078-58885097	Msa0740060:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	58881472	58885374	58881472	ID=Msa0740060;Name=Msa0740060
chr5_1	mRNA	58881472	58885374	58881472	ID=Msa0740060-mRNA-1;Parent=Msa0740060;Name=Msa0740060-mRNA-1;_AED=0.02;_eAED=0.02;_QI=114\|0.25\|0.4\|1\|1\|1\|5\|0\|797
chr5_1	exon	58881472	58881850	58881472	ID=Msa0740060-mRNA-1:exon:16810;Parent=Msa0740060-mRNA-1
chr5_1	exon	58882204	58882286	58882204	ID=Msa0740060-mRNA-1:exon:16809;Parent=Msa0740060-mRNA-1
chr5_1	exon	58882387	58882460	58882387	ID=Msa0740060-mRNA-1:exon:16808;Parent=Msa0740060-mRNA-1
chr5_1	exon	58882712	58884415	58882712	ID=Msa0740060-mRNA-1:exon:16807;Parent=Msa0740060-mRNA-1
chr5_1	exon	58885107	58885374	58885107	ID=Msa0740060-mRNA-1:exon:16806;Parent=Msa0740060-mRNA-1
chr5_1	five_prime_UTR	58885261	58885374	58885261	ID=Msa0740060-mRNA-1:five_prime_utr;Parent=Msa0740060-mRNA-1
chr5_1	CDS	58885107	58885260	58885107	ID=Msa0740060-mRNA-1:cds;Parent=Msa0740060-mRNA-1
chr5_1	CDS	58882712	58884415	58882712	ID=Msa0740060-mRNA-1:cds;Parent=Msa0740060-mRNA-1
chr5_1	CDS	58882387	58882460	58882387	ID=Msa0740060-mRNA-1:cds;Parent=Msa0740060-mRNA-1
chr5_1	CDS	58882204	58882286	58882204	ID=Msa0740060-mRNA-1:cds;Parent=Msa0740060-mRNA-1
chr5_1	CDS	58881472	58881850	58881472	ID=Msa0740060-mRNA-1:cds;Parent=Msa0740060-mRNA-1

Gene Sequence

>Msa0740060

ATGGACGTGAAGAAGAGTGAGAATCAAACAAGTTTGATTTTCTGTGTCAATGGTGAAAAGTTTGAGTTATCCAAAGTTGATCCGTCAACCACTTTGCTTGAGTTCTTACGTACTCAAACTCAATTCAAGAGTGTTAAACTCGGTTGTGGTGAAGGTGGTTGCGGAGCTTGTGTAGTTTTAGTCTCCAAATATGATCCATTGCTCGACAGAGTTGAGGATTTTACCGCGAGCTCTTGCCTCACACTACTTTGTAGCATACATGGATGTTCAATAACAACAAGTGAAGGCATTGGAAACAGCAAACAAGGATTCCATTCAATTCATGAAAGATTTGCAGGATTCCATGCTTCGCAATGCGGTTTCTGTACTCCCGGAATGTCTGTTTCCCTCTTCGGTGCTCTTGTCAACGCTGACAAGAGCAATTCTCCTGAGCCACCACCTGGTGTCTCAAAAATCACCGTTTCCAAGGCTGAAAAATCTATTGCAGGGAATCTTTGTCGCTGCACTGGTTACCGACCGATTGCTGATGCCTGCAAAAGTTTTGCAGCTGATGTTGATATGGAGGATCTCGGTTTGAACTCTTTCTGGAAAAAGGGAGAGAGCAAAGAATCGAAGCTTAGTAAGTTGCCTCGATACGATCGTGATCACAAGAATATCGAATTTCCTATGTTTTTGAAGGAAGTCAAACCTGATTTGTTTATAGCTTCTGAGAAACATAGTTGGAATAAACCTACTAGTTTAGAGGAGCTTCAAAGTTTATTGGAATTAAACCATGCCAATGAAACAAAGATAAAAGTTGTTGTTAATAATACTGGTATGGGATATTACAAAGATAAACAAGGCTATGATAAGTATATTGATATAAGTGGAATTTCTGAGCTTTCAAAAATCAAAAGGGATCAATCAGGGATTGAAATTGGAGCAGCGGTGAGTATATCTAAAGCAATTGAAGCATTACAGAAAGAAAGCACGAGTGATTTTATCTCAGATTTTGTGATGATACTTGAAAAGATTGCTGATCATATGAGCAAAGTTGCGACGGGTTTTATTCGGAACACGGCTAGTGTAGGTGGCAATCTGGTGATGGCGCAAAAGAATAAGTTTCCTTCGGATATAGTTACTATACTTCTTGCTGTGGATTCAATGGTTCACATAATGACTGGTGCAAAGTTTGAATGGCTTGCATTGGAGGAGTTTCTTGAGAGACCACCGTTAGGTTTTGAAAGTGTTCTTTTAAGCATTAAGATTCCTAGTATCGAACTCTATACAAGCGAATCTTCGGAGACAAGAAGTAGTAAAGTCCTCTTTGAAACCTACCGTGCTTCTCCTCGACCGCTTGGAAACGCGCTTTCTTACTTAAATGCTGCTTTCCTTGTTCAGGTGTCTCCTTGCAAGGATACTCATGAAACCATGATAGATACTTGTAGGTTGTCTTTTGGTGGTTTTAGGAATAAGCATGCAATCAGAGCTAAACATGTTGAGAAGTTCTTATCAGGAAAGTCGTTAAGTGTTAGCAACCTGTATGAAGCTATCAACTTGCTTACAGATACTGCCGCTATTACACCTCAAGATGAAACCTTGACACATGGTTACCTTTCGAGTTTAGCAGTTGGTTTTATTTTTCAGTTCTTTAATTCACAGATTGAAAGTCCTGCAAGGATAACCAATGGTTATTTAAATGGATATACTCATTTGCCTTCGGCCGAGGCTTCCGACATAAAAGAGAATCAGAAGCAGGTGAAGCATAACAGATTTCCTACTTTATTGTCGTCCGGGAAGCAAGTCTTTGAAGCAGGCACCGAGTATAATCCGATTGGCGAGCCAGTCACAAAATCTGGTGCTGCGCTACAAGCTTCAGTTGGTCAACTTGTTGGTCAACTAGCAAAATTGATGGGATGTTATGTTGTTGGAAGTGCTGGAAGTAAATATAAGGTTGATATTTTGAAGACCAAGTTTGGGTTTGATGAGGCCTTTAATTACAAGGAGGAACAAGACCTAGATGGAACATTGAAGAAGTACTTTCCTGAAGGAATAGACATATACTTTGACAATGTTGGTGGAGACATGCTTGAAGCAGCCCTTCTTAACATGAGACGCCGCGGTCGAATTGTGGTGGCTGGAATGATCTCACAATATGAACTTGATGAACCTCAAGGCATAAAAAACTTAATCAACATCATATACAAGCAGATTCAAGTAGATGCATTCACAGTATATGATTACTATCACTTATATCCAAAGTTTTTGGACACAATTTTGCCTTATGTTAGGGAAGGGAAGATGACATATGTTGAAGATATAGCTAAAGGGATTGAGAGTGGGCCAGCTGCATTAGAAGCAATGTTCACAGGCAAAAGTGCTGGCAAACAAGTTGTTTTAGTTGCTGATGAATAA

Protein sequence

>Msa0740060

MDVKKSENQTSLIFCVNGEKFELSKVDPSTTLLEFLRTQTQFKSVKLGCGEGGCGACVVLVSKYDPLLDRVEDFTASSCLTLLCSIHGCSITTSEGIGNSKQGFHSIHERFAGFHASQCGFCTPGMSVSLFGALVNADKSNSPEPPPGVSKITVSKAEKSIAGNLCRCTGYRPIADACKSFAADVDMEDLGLNSFWKKGESKESKLSKLPRYDRDHKNIEFPMFLKEVKPDLFIASEKHSWNKPTSLEELQSLLELNHANETKIKVVVNNTGMGYYKDKQGYDKYIDISGISELSKIKRDQSGIEIGAAVSISKAIEALQKESTSDFISDFVMILEKIADHMSKVATGFIRNTASVGGNLVMAQKNKFPSDIVTILLAVDSMVHIMTGAKFEWLALEEFLERPPLGFESVLLSIKIPSIELYTSESSETRSSKVLFETYRASPRPLGNALSYLNAAFLVQVSPCKDTHETMIDTCRLSFGGFRNKHAIRAKHVEKFLSGKSLSVSNLYEAINLLTDTAAITPQDETLTHGYLSSLAVGFIFQFFNSQIESPARITNGYLNGYTHLPSAEASDIKENQKQVKHNRFPTLLSSGKQVFEAGTEYNPIGEPVTKSGAALQASVGQLVGQLAKLMGCYVVGSAGSKYKVDILKTKFGFDEAFNYKEEQDLDGTLKKYFPEGIDIYFDNVGGDMLEAALLNMRRRGRIVVAGMISQYELDEPQGIKNLINIIYKQIQVDAFTVYDYYHLYPKFLDTILPYVREGKMTYVEDIAKGIESGPAALEAMFTGKSAGKQVVLVADE*