AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0585650


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585650 MtrunA17_Chr4g0001531 100.000 57 0 0 21 77 1 57 1.67e-38 122
Msa0585650 MtrunA17_Chr7g0214441 100.000 57 0 0 21 77 1 57 1.67e-38 122
Msa0585650 MtrunA17_Chr4g0024331 38.272 81 42 2 1 76 36 113 1.72e-15 65.5
Msa0585650 MtrunA17_Chr2g0322741 37.037 81 43 2 1 76 37 114 8.67e-15 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585650 AT3G05870.1 100.000 76 0 0 1 76 11 86 1.67e-53 160
Msa0585650 AT3G05870.2 100.000 76 0 0 1 76 11 86 1.67e-53 160
Msa0585650 AT3G05870.6 100.000 76 0 0 1 76 11 86 1.67e-53 160
Msa0585650 AT3G05870.4 100.000 76 0 0 1 76 14 89 2.07e-53 160
Msa0585650 AT3G05870.3 100.000 76 0 0 1 76 14 89 2.07e-53 160
Msa0585650 AT3G05870.5 100.000 76 0 0 1 76 14 89 2.07e-53 160
Msa0585650 AT3G05870.7 100.000 76 0 0 1 76 2 77 5.89e-53 159
Msa0585650 AT5G20570.1 38.272 81 42 2 1 76 37 114 3.25e-15 65.1
Msa0585650 AT5G26640.1 84.375 32 5 0 8 39 5 36 2.30e-14 61.6
Msa0585650 AT3G42830.1 38.272 81 42 2 1 76 34 111 3.97e-14 62.4

Find 26 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTGTTGCTTCATGGACT+TGG 0.279744 4_2:+6694543 Msa0585650:CDS
CGTCGGGAATGGCAATTTAA+AGG 0.290239 4_2:+6695520 Msa0585650:CDS
ATTTGCATTGTATCTTGAAA+TGG 0.352612 4_2:+6695458 Msa0585650:CDS
TGTCCTGATTGTAAACTTCC+CGG 0.396496 4_2:+6694617 Msa0585650:CDS
GATGGCATGCTGTTGCTTCA+TGG 0.397141 4_2:+6694537 None:intergenic
GCCATTCCCGACGGCACATT+GGG 0.397444 4_2:-6695510 None:intergenic
ATTTGTAGGATGGCCTTTGA+TGG 0.411828 4_2:+6694590 Msa0585650:CDS
TGCTGTTGCTTCATGGACTT+GGG 0.419431 4_2:+6694544 Msa0585650:CDS
ATGAAACATGTGGGATTTGT+AGG 0.429235 4_2:+6694576 Msa0585650:CDS
TGCCATTCCCGACGGCACAT+TGG 0.451421 4_2:-6695511 None:intergenic
ACTGAGCGGGCAGTCATCCC+CGG 0.466023 4_2:-6694636 None:intergenic
GTCCTGATTGTAAACTTCCC+GGG 0.472599 4_2:+6694618 Msa0585650:CDS
GCCCAATGTGCCGTCGGGAA+TGG 0.481653 4_2:+6695509 Msa0585650:CDS
TTTGCATTGTATCTTGAAAT+GGG 0.482104 4_2:+6695459 Msa0585650:CDS
CACATTGCCCAATGTGCCGT+CGG 0.515957 4_2:+6695503 Msa0585650:CDS
TCCCCGGGAAGTTTACAATC+AGG 0.518100 4_2:-6694620 None:intergenic
CTTTAAATTGCCATTCCCGA+CGG 0.544857 4_2:-6695519 None:intergenic
CATTGGGCAATGTGCTTGTG+AGG 0.551130 4_2:-6695494 None:intergenic
ACATTGCCCAATGTGCCGTC+GGG 0.562769 4_2:+6695504 Msa0585650:CDS
CTGAGCGGGCAGTCATCCCC+GGG 0.565342 4_2:-6694635 None:intergenic
TCCTGATTGTAAACTTCCCG+GGG 0.576107 4_2:+6694619 Msa0585650:CDS
TTAGCAGTAGCTTACTGAGC+GGG 0.577553 4_2:-6694649 None:intergenic
ATCAGGACAACATCCATCAA+AGG 0.626104 4_2:-6694603 None:intergenic
AACATGTGGGATTTGTAGGA+TGG 0.654714 4_2:+6694580 Msa0585650:CDS
GATGCACAAGATGAAACATG+TGG 0.720145 4_2:+6694566 Msa0585650:CDS
ATGCACAAGATGAAACATGT+GGG 0.725526 4_2:+6694567 Msa0585650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAGTTTCATAAATATAATT+AGG + chr4_2:6695059-6695078 Msa0585650:intron 15.0%
!! GTAGTTTCATAAATATAATT+AGG + chr4_2:6695059-6695078 Msa0585650:intron 15.0%
!! TATTTGCATAAATTTGTCTT+TGG + chr4_2:6694703-6694722 Msa0585650:intron 20.0%
!!! TCAGTTCATTTCTAATTTTT+CGG + chr4_2:6695000-6695019 Msa0585650:intron 20.0%
!!! TTATAAAAGCTTTATTCTCA+AGG + chr4_2:6695129-6695148 Msa0585650:intron 20.0%
!!! GTAATTGACAATTCTATTTT+GGG + chr4_2:6695205-6695224 Msa0585650:intron 20.0%
!! TATTTGCATAAATTTGTCTT+TGG + chr4_2:6694703-6694722 Msa0585650:intron 20.0%
!!! TCAGTTCATTTCTAATTTTT+CGG + chr4_2:6695000-6695019 Msa0585650:intron 20.0%
!!! TTATAAAAGCTTTATTCTCA+AGG + chr4_2:6695129-6695148 Msa0585650:intron 20.0%
!!! GTAATTGACAATTCTATTTT+GGG + chr4_2:6695205-6695224 Msa0585650:intron 20.0%
!! AACTAAAAGTCTCTTTTCTA+AGG - chr4_2:6694870-6694889 None:intergenic 25.0%
!!! TAATTTTTCGGAATGTAGAT+AGG + chr4_2:6695012-6695031 Msa0585650:intron 25.0%
! AAACTACAAATGATGTTTAG+AGG - chr4_2:6695046-6695065 None:intergenic 25.0%
! TGAAAAATTCTCAATTATGC+AGG - chr4_2:6695169-6695188 None:intergenic 25.0%
! AATTGTCAATTACCATCTTA+TGG - chr4_2:6695198-6695217 None:intergenic 25.0%
!! GGTAATTGACAATTCTATTT+TGG + chr4_2:6695204-6695223 Msa0585650:intron 25.0%
! AGAATCATATTTGATCAGTT+AGG - chr4_2:6695357-6695376 None:intergenic 25.0%
! ATTTGCATTGTATCTTGAAA+TGG + chr4_2:6695458-6695477 Msa0585650:CDS 25.0%
! TTTGCATTGTATCTTGAAAT+GGG + chr4_2:6695459-6695478 Msa0585650:CDS 25.0%
!! AACTAAAAGTCTCTTTTCTA+AGG - chr4_2:6694870-6694889 None:intergenic 25.0%
!!! TAATTTTTCGGAATGTAGAT+AGG + chr4_2:6695012-6695031 Msa0585650:intron 25.0%
! AAACTACAAATGATGTTTAG+AGG - chr4_2:6695046-6695065 None:intergenic 25.0%
! TGAAAAATTCTCAATTATGC+AGG - chr4_2:6695169-6695188 None:intergenic 25.0%
! AATTGTCAATTACCATCTTA+TGG - chr4_2:6695198-6695217 None:intergenic 25.0%
!! GGTAATTGACAATTCTATTT+TGG + chr4_2:6695204-6695223 Msa0585650:intron 25.0%
! AGAATCATATTTGATCAGTT+AGG - chr4_2:6695357-6695376 None:intergenic 25.0%
! ATTTGCATTGTATCTTGAAA+TGG + chr4_2:6695458-6695477 Msa0585650:CDS 25.0%
! TTTGCATTGTATCTTGAAAT+GGG + chr4_2:6695459-6695478 Msa0585650:CDS 25.0%
GTAAAGCTTAACTTCATAAC+TGG + chr4_2:6694776-6694795 Msa0585650:intron 30.0%
CTACAAAGATGTATTGTTAC+TGG + chr4_2:6695090-6695109 Msa0585650:intron 30.0%
!! AAGCTTTATTCTCAAGGAAT+TGG + chr4_2:6695135-6695154 Msa0585650:intron 30.0%
AATTCTCAAGCATGATTTCA+CGG - chr4_2:6695231-6695250 None:intergenic 30.0%
AATGGCAATTTAAAGGTTGA+TGG + chr4_2:6695527-6695546 Msa0585650:CDS 30.0%
GTAAAGCTTAACTTCATAAC+TGG + chr4_2:6694776-6694795 Msa0585650:intron 30.0%
CTACAAAGATGTATTGTTAC+TGG + chr4_2:6695090-6695109 Msa0585650:intron 30.0%
!! AAGCTTTATTCTCAAGGAAT+TGG + chr4_2:6695135-6695154 Msa0585650:intron 30.0%
AATTCTCAAGCATGATTTCA+CGG - chr4_2:6695231-6695250 None:intergenic 30.0%
ATGCACAAGATGAAACATGT+GGG + chr4_2:6694567-6694586 Msa0585650:CDS 35.0%
ATGAAACATGTGGGATTTGT+AGG + chr4_2:6694576-6694595 Msa0585650:CDS 35.0%
!!! TTTTTGAGTTCTCTACATGC+AGG + chr4_2:6694965-6694984 Msa0585650:intron 35.0%
! TTTTCAGAAGAGCCATAAGA+TGG + chr4_2:6695183-6695202 Msa0585650:intron 35.0%
AGAAACAAAGACAGACATCA+CGG - chr4_2:6695265-6695284 None:intergenic 35.0%
! TGATGTCTGTCTTTGTTTCT+CGG + chr4_2:6695265-6695284 Msa0585650:intron 35.0%
! GCATAACTTGTCTTTCTCTA+CGG + chr4_2:6695307-6695326 Msa0585650:intron 35.0%
! TTTTCCTACTACTTGCAGTT+TGG + chr4_2:6695413-6695432 Msa0585650:intron 35.0%
TTTCCTACTACTTGCAGTTT+GGG + chr4_2:6695414-6695433 Msa0585650:intron 35.0%
ATGCACAAGATGAAACATGT+GGG + chr4_2:6694567-6694586 Msa0585650:CDS 35.0%
ATGAAACATGTGGGATTTGT+AGG + chr4_2:6694576-6694595 Msa0585650:CDS 35.0%
!!! TTTTTGAGTTCTCTACATGC+AGG + chr4_2:6694965-6694984 Msa0585650:intron 35.0%
! TTTTCAGAAGAGCCATAAGA+TGG + chr4_2:6695183-6695202 Msa0585650:intron 35.0%
AGAAACAAAGACAGACATCA+CGG - chr4_2:6695265-6695284 None:intergenic 35.0%
! TGATGTCTGTCTTTGTTTCT+CGG + chr4_2:6695265-6695284 Msa0585650:intron 35.0%
! GCATAACTTGTCTTTCTCTA+CGG + chr4_2:6695307-6695326 Msa0585650:intron 35.0%
! TTTTCCTACTACTTGCAGTT+TGG + chr4_2:6695413-6695432 Msa0585650:intron 35.0%
TTTCCTACTACTTGCAGTTT+GGG + chr4_2:6695414-6695433 Msa0585650:intron 35.0%
GATGCACAAGATGAAACATG+TGG + chr4_2:6694566-6694585 Msa0585650:CDS 40.0%
AACATGTGGGATTTGTAGGA+TGG + chr4_2:6694580-6694599 Msa0585650:CDS 40.0%
ATTTGTAGGATGGCCTTTGA+TGG + chr4_2:6694590-6694609 Msa0585650:CDS 40.0%
ATCAGGACAACATCCATCAA+AGG - chr4_2:6694606-6694625 None:intergenic 40.0%
TGTCCTGATTGTAAACTTCC+CGG + chr4_2:6694617-6694636 Msa0585650:CDS 40.0%
ATTAGCAGTAGCTTACTGAG+CGG - chr4_2:6694653-6694672 None:intergenic 40.0%
GCTACTGCTAATGTTGTCTT+TGG + chr4_2:6694661-6694680 Msa0585650:intron 40.0%
TTTCGGAATGTAGATAGGTC+TGG + chr4_2:6695017-6695036 Msa0585650:intron 40.0%
TGTATTGTTACTGGCATGCA+AGG + chr4_2:6695099-6695118 Msa0585650:intron 40.0%
TTCCTACTACTTGCAGTTTG+GGG + chr4_2:6695415-6695434 Msa0585650:intron 40.0%
CTTTAAATTGCCATTCCCGA+CGG - chr4_2:6695522-6695541 None:intergenic 40.0%
GATGCACAAGATGAAACATG+TGG + chr4_2:6694566-6694585 Msa0585650:CDS 40.0%
AACATGTGGGATTTGTAGGA+TGG + chr4_2:6694580-6694599 Msa0585650:CDS 40.0%
ATTTGTAGGATGGCCTTTGA+TGG + chr4_2:6694590-6694609 Msa0585650:CDS 40.0%
ATCAGGACAACATCCATCAA+AGG - chr4_2:6694606-6694625 None:intergenic 40.0%
TGTCCTGATTGTAAACTTCC+CGG + chr4_2:6694617-6694636 Msa0585650:CDS 40.0%
ATTAGCAGTAGCTTACTGAG+CGG - chr4_2:6694653-6694672 None:intergenic 40.0%
GCTACTGCTAATGTTGTCTT+TGG + chr4_2:6694661-6694680 Msa0585650:intron 40.0%
TTTCGGAATGTAGATAGGTC+TGG + chr4_2:6695017-6695036 Msa0585650:intron 40.0%
TGTATTGTTACTGGCATGCA+AGG + chr4_2:6695099-6695118 Msa0585650:intron 40.0%
TTCCTACTACTTGCAGTTTG+GGG + chr4_2:6695415-6695434 Msa0585650:intron 40.0%
CTTTAAATTGCCATTCCCGA+CGG - chr4_2:6695522-6695541 None:intergenic 40.0%
! ATGCTGTTGCTTCATGGACT+TGG + chr4_2:6694543-6694562 Msa0585650:CDS 45.0%
! TGCTGTTGCTTCATGGACTT+GGG + chr4_2:6694544-6694563 Msa0585650:CDS 45.0%
GTCCTGATTGTAAACTTCCC+GGG + chr4_2:6694618-6694637 Msa0585650:CDS 45.0%
TCCTGATTGTAAACTTCCCG+GGG + chr4_2:6694619-6694638 Msa0585650:CDS 45.0%
TTAGCAGTAGCTTACTGAGC+GGG - chr4_2:6694652-6694671 None:intergenic 45.0%
AACAAAGACAGACATCACGG+CGG - chr4_2:6695262-6695281 None:intergenic 45.0%
CGTCGGGAATGGCAATTTAA+AGG + chr4_2:6695520-6695539 Msa0585650:CDS 45.0%
! ATGCTGTTGCTTCATGGACT+TGG + chr4_2:6694543-6694562 Msa0585650:CDS 45.0%
! TGCTGTTGCTTCATGGACTT+GGG + chr4_2:6694544-6694563 Msa0585650:CDS 45.0%
GTCCTGATTGTAAACTTCCC+GGG + chr4_2:6694618-6694637 Msa0585650:CDS 45.0%
TCCTGATTGTAAACTTCCCG+GGG + chr4_2:6694619-6694638 Msa0585650:CDS 45.0%
TTAGCAGTAGCTTACTGAGC+GGG - chr4_2:6694652-6694671 None:intergenic 45.0%
AACAAAGACAGACATCACGG+CGG - chr4_2:6695262-6695281 None:intergenic 45.0%
CGTCGGGAATGGCAATTTAA+AGG + chr4_2:6695520-6695539 Msa0585650:CDS 45.0%
TCCCCGGGAAGTTTACAATC+AGG - chr4_2:6694623-6694642 None:intergenic 50.0%
CACCCCAAACTGCAAGTAGT+AGG - chr4_2:6695420-6695439 None:intergenic 50.0%
CATTGGGCAATGTGCTTGTG+AGG - chr4_2:6695497-6695516 None:intergenic 50.0%
TCCCCGGGAAGTTTACAATC+AGG - chr4_2:6694623-6694642 None:intergenic 50.0%
CACCCCAAACTGCAAGTAGT+AGG - chr4_2:6695420-6695439 None:intergenic 50.0%
CATTGGGCAATGTGCTTGTG+AGG - chr4_2:6695497-6695516 None:intergenic 50.0%
CACATTGCCCAATGTGCCGT+CGG + chr4_2:6695503-6695522 Msa0585650:CDS 55.0%
ACATTGCCCAATGTGCCGTC+GGG + chr4_2:6695504-6695523 Msa0585650:CDS 55.0%
CACATTGCCCAATGTGCCGT+CGG + chr4_2:6695503-6695522 Msa0585650:CDS 55.0%
ACATTGCCCAATGTGCCGTC+GGG + chr4_2:6695504-6695523 Msa0585650:CDS 55.0%
GCCATTCCCGACGGCACATT+GGG - chr4_2:6695513-6695532 None:intergenic 60.0%
TGCCATTCCCGACGGCACAT+TGG - chr4_2:6695514-6695533 None:intergenic 60.0%
GCCATTCCCGACGGCACATT+GGG - chr4_2:6695513-6695532 None:intergenic 60.0%
TGCCATTCCCGACGGCACAT+TGG - chr4_2:6695514-6695533 None:intergenic 60.0%
ACTGAGCGGGCAGTCATCCC+CGG - chr4_2:6694639-6694658 None:intergenic 65.0%
GCCCAATGTGCCGTCGGGAA+TGG + chr4_2:6695509-6695528 Msa0585650:CDS 65.0%
ACTGAGCGGGCAGTCATCCC+CGG - chr4_2:6694639-6694658 None:intergenic 65.0%
GCCCAATGTGCCGTCGGGAA+TGG + chr4_2:6695509-6695528 Msa0585650:CDS 65.0%
CTGAGCGGGCAGTCATCCCC+GGG - chr4_2:6694638-6694657 None:intergenic 70.0%
CTGAGCGGGCAGTCATCCCC+GGG - chr4_2:6694638-6694657 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 6694539 6695546 6694539 ID=Msa0585650;Name=Msa0585650
chr4_2 mRNA 6694539 6695546 6694539 ID=Msa0585650-mRNA-1;Parent=Msa0585650;Name=Msa0585650-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|0|0.5|2|0|77
chr4_2 exon 6694539 6694656 6694539 ID=Msa0585650-mRNA-1:exon:1630;Parent=Msa0585650-mRNA-1
chr4_2 exon 6695431 6695546 6695431 ID=Msa0585650-mRNA-1:exon:1631;Parent=Msa0585650-mRNA-1
chr4_2 CDS 6694539 6694656 6694539 ID=Msa0585650-mRNA-1:cds;Parent=Msa0585650-mRNA-1
chr4_2 CDS 6695431 6695546 6695431 ID=Msa0585650-mRNA-1:cds;Parent=Msa0585650-mRNA-1
Gene Sequence

>Msa0585650

TGGCATGCTGTTGCTTCATGGACTTGGGATGCACAAGATGAAACATGTGGGATTTGTAGGATGGCCTTTGATGGATGTTGTCCTGATTGTAAACTTCCCGGGGATGACTGCCCGCTCATTTGGGGTGCATGCAATCATGCATTTCATTTGCATTGTATCTTGAAATGGGTGAATTCTCAGACCTCACAAGCACATTGCCCAATGTGCCGTCGGGAATGGCAATTTAAAGGTTGA

Protein sequence

>Msa0585650

WHAVASWTWDAQDETCGICRMAFDGCCPDCKLPGDDCPLIWGACNHAFHLHCILKWVNSQTSQAHCPMCRREWQFKG*