AlfalfaGEDB | Msa0452810

M. sativa cultivar zhongmu-4 / Msa0452810

KEGG: K13108
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008283 GO:0009507 GO:0009536 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0019222 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040008 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048519 GO:0048638 GO:0050789 GO:0050793 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901698 GO:1901699
PFAM: PF00498.29

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0452810	XP_024633593.1	100.000	60	0	0	1	60	205	264	9.11e-35	128

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0452810	sp\|Q8W4D8\|DDL_ARATH	84.211	57	9	0	1	57	257	313	5.44e-29	107

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0452810	G7J1A2	100.000	60	0	0	1	60	267	326	1.31e-34	128

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0050930	Msa0452810	-0.810589	1.069253e-50	-8.615850e-47
Msa0094600	Msa0452810	0.800541	1.373172e-48	-8.615850e-47
Msa0133980	Msa0452810	0.830585	2.702701e-55	-8.615850e-47
Msa0158130	Msa0452810	0.808773	2.626827e-50	-8.615850e-47
Msa0175050	Msa0452810	-0.800771	1.232514e-48	-8.615850e-47
Msa0365490	Msa0452810	-0.824267	8.854658e-54	-8.615850e-47
Msa0388030	Msa0452810	0.815707	8.051744e-52	-8.615850e-47
Msa0452810	Msa0458440	-0.829638	4.603337e-55	-8.615850e-47
Msa0452810	Msa0505060	-0.803302	3.719162e-49	-8.615850e-47
Msa0452810	Msa0570640	-0.805925	1.054916e-49	-8.615850e-47
Msa0452810	Msa0585620	0.834070	3.706157e-56	-8.615850e-47
Msa0452810	Msa0585650	0.827113	1.871546e-54	-8.615850e-47
Msa0452810	Msa0648520	-0.804288	2.321399e-49	-8.615850e-47
Msa0452810	Msa0691050	-0.816961	4.222372e-52	-8.615850e-47
Msa0452810	Msa0711130	-0.815784	7.740433e-52	-8.615850e-47
Msa0452810	Msa0716860	-0.803940	2.742015e-49	-8.615850e-47
Msa0452810	Msa0740060	-0.823678	1.217139e-53	-8.615850e-47
Msa0452810	Msa0799060	-0.855849	4.784875e-62	-8.615850e-47
Msa0452810	Msa0959010	-0.804425	2.173444e-49	-8.615850e-47
Msa0452810	Msa0993530	-0.809598	1.748406e-50	-8.615850e-47
Msa0452810	Msa1021510	-0.803253	3.808127e-49	-8.615850e-47
Msa0452810	Msa1031230	-0.804504	2.092615e-49	-8.615850e-47
Msa0452810	Msa1152950	-0.816588	5.118897e-52	-8.615850e-47
Msa0452810	Msa1174310	-0.815178	1.055798e-51	-8.615850e-47
Msa0452810	Msa1263290	0.806947	6.423271e-50	-8.615850e-47
Msa0452810	Msa1314770	-0.802201	6.277193e-49	-8.615850e-47
Msa0452810	Msa1338250	-0.818954	1.497208e-52	-8.615850e-47
Msa0452810	Msa1352540	0.816597	5.095668e-52	-8.615850e-47
Msa0452810	Msa1373350	-0.801628	8.231723e-49	-8.615850e-47
Msa0452810	Msa1390120	-0.802805	4.713679e-49	-8.615850e-47
Msa0452810	Msa1395530	-0.805082	1.584857e-49	-8.615850e-47
Msa0452810	Msa1422840	-0.815312	9.862008e-52	-8.615850e-47
Msa0452810	Msa1433220	-0.808561	2.914992e-50	-8.615850e-47
Msa0452810	Msa1435400	-0.800157	1.644138e-48	-8.615850e-47
Msa0265230	Msa0452810	0.821152	4.699678e-53	-8.615850e-47
Msa0277420	Msa0452810	-0.803394	3.560391e-49	-8.615850e-47
Msa0319130	Msa0452810	-0.812375	4.376185e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0452810	MtrunA17_Chr3g0097951	100.000	60	0	0	1	60	267	326	2.53e-38	128

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0452810	AT3G20550.1	84.211	57	9	0	1	57	257	313	5.53e-30	107

Find 44 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTACTATTACCAAATTTAA+TGG	0.139667	3_3:+42229218	None:intergenic
TATGGTTAACGTCATTAATT+TGG	0.189898	3_3:-42228296	Msa0452810:three_prime_UTR
CCCTTCTTTCTCTTCCAAAA+AGG	0.238271	3_3:+42229773	None:intergenic
GAGCAGGACACCATTAAATT+TGG	0.277561	3_3:-42229228	Msa0452810:CDS
ACTTTGGCGATGTATATATA+GGG	0.313226	3_3:+42229801	None:intergenic
AACTTTGGCGATGTATATAT+AGG	0.346821	3_3:+42229800	None:intergenic
CTGCAATAGGCCTTACATAA+TGG	0.359905	3_3:-42229448	Msa0452810:intron
AAGGCTCTGACATTCTCATT+TGG	0.389171	3_3:+42228435	None:intergenic
TGAGCAGGATGGTACAGATT+TGG	0.394070	3_3:+42228589	None:intergenic
CTTTCCTTCCATATCTTCAA+AGG	0.397753	3_3:+42228395	None:intergenic
TGCTTTACTATCATACCATC+AGG	0.432186	3_3:+42229566	None:intergenic
CCATTAAATTTGGTAATAGT+AGG	0.444737	3_3:-42229218	Msa0452810:intron
AACATGCTGTTATTCAATTC+CGG	0.445183	3_3:-42229711	Msa0452810:intron
CTGCAAGATGGATGATCTGT+TGG	0.448480	3_3:+42229739	None:intergenic
ATAATACCGTTGAGGTTCAA+TGG	0.452577	3_3:+42229260	None:intergenic
TCTTATTAGGCAAGTTGAAA+AGG	0.459403	3_3:-42229594	Msa0452810:intron
AAGAGGGAATACACAAAACT+TGG	0.470484	3_3:+42228518	None:intergenic
TTTCCTTCCATATCTTCAAA+GGG	0.471949	3_3:+42228396	None:intergenic
TTTGATCACTTCTGATGAGC+AGG	0.474013	3_3:+42228574	None:intergenic
CTCAACGGTATTATGAACTT+AGG	0.474206	3_3:-42229251	Msa0452810:CDS
CCTACAATCTTTCTGAAAAG+AGG	0.476074	3_3:+42228501	None:intergenic
CGTGCAGTAATACATATTCT+CGG	0.478798	3_3:+42228650	None:intergenic
GAAATGCCCCTTTGAAGATA+TGG	0.484867	3_3:-42228403	Msa0452810:three_prime_UTR
TAATACCGTTGAGGTTCAAT+GGG	0.484980	3_3:+42229261	None:intergenic
TTAACGTCATTAATTTGGTA+AGG	0.501502	3_3:-42228291	None:intergenic
CTTCCGAGGTCCATTATGTA+AGG	0.513040	3_3:+42229438	None:intergenic
CTACAATCTTTCTGAAAAGA+GGG	0.520508	3_3:+42228502	None:intergenic
TCAACGGTATTATGAACTTA+GGG	0.530079	3_3:-42229250	Msa0452810:CDS
TTTGAAGATATGGAAGGAAA+GGG	0.533558	3_3:-42228393	Msa0452810:three_prime_UTR
CTTTGAAGATATGGAAGGAA+AGG	0.546320	3_3:-42228394	Msa0452810:three_prime_UTR
GTATTATGAACTTAGGGAGC+AGG	0.563275	3_3:-42229244	Msa0452810:CDS
GCATGTTGCTTGCTGCAAGA+TGG	0.571521	3_3:+42229727	None:intergenic
GGGGCATTTCGCTTAAGCAA+AGG	0.588812	3_3:+42228416	None:intergenic
TGCAAGATGGATGATCTGTT+GGG	0.594043	3_3:+42229740	None:intergenic
CTAAGTTCATAATACCGTTG+AGG	0.612664	3_3:+42229252	None:intergenic
CTTTGGCGATGTATATATAG+GGG	0.612788	3_3:+42229802	None:intergenic
GTTGAAAAGGAGCAACCTGA+TGG	0.624219	3_3:-42229581	Msa0452810:five_prime_UTR
ATCACTTCTGATGAGCAGGA+TGG	0.626882	3_3:+42228578	None:intergenic
TGCCCCTTTGAAGATATGGA+AGG	0.629402	3_3:-42228399	Msa0452810:three_prime_UTR
GTATGATAGTAAAGCAAACA+AGG	0.635651	3_3:-42229559	Msa0452810:intron
TTCCTTCCATATCTTCAAAG+GGG	0.646574	3_3:+42228397	None:intergenic
ATAGTCCCATTGAACCTCAA+CGG	0.667088	3_3:-42229266	Msa0452810:CDS
AGGCCTTACATAATGGACCT+CGG	0.670855	3_3:-42229441	Msa0452810:CDS
AGGATGGTACAGATTTGGAG+AGG	0.702925	3_3:+42228594	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATAATGAAATTTTTCTAAA+TGG	+	chr3_3:42229034-42229053	None:intergenic	10.0%
!!	TATTGTTCAAAAATAAAAAA+AGG	-	chr3_3:42229196-42229215	Msa0452810:intron	10.0%
!!!	TATAATGAAATTTTTCTAAA+TGG	+	chr3_3:42229034-42229053	None:intergenic	10.0%
!!	TATTGTTCAAAAATAAAAAA+AGG	-	chr3_3:42229196-42229215	Msa0452810:intron	10.0%
!!!	GAAATTTTTCTAAATGGAAT+TGG	+	chr3_3:42229028-42229047	None:intergenic	20.0%
!!	GTTTATTGTCAGTAAAAAAA+TGG	-	chr3_3:42229165-42229184	Msa0452810:intron	20.0%
!!	ATGAATAATATTTGAATCCA+TGG	-	chr3_3:42229312-42229331	Msa0452810:intron	20.0%
!!!	TACATTTATATTGGTTAACA+GGG	+	chr3_3:42229410-42229429	None:intergenic	20.0%
!!!	ATACATTTATATTGGTTAAC+AGG	+	chr3_3:42229411-42229430	None:intergenic	20.0%
!!!	GAAATTTTTCTAAATGGAAT+TGG	+	chr3_3:42229028-42229047	None:intergenic	20.0%
!!	GTTTATTGTCAGTAAAAAAA+TGG	-	chr3_3:42229165-42229184	Msa0452810:intron	20.0%
!!	ATGAATAATATTTGAATCCA+TGG	-	chr3_3:42229312-42229331	Msa0452810:intron	20.0%
!!!	TACATTTATATTGGTTAACA+GGG	+	chr3_3:42229410-42229429	None:intergenic	20.0%
!!!	ATACATTTATATTGGTTAAC+AGG	+	chr3_3:42229411-42229430	None:intergenic	20.0%
!	TTCCAAAAAGGTAATAACTT+TGG	+	chr3_3:42228336-42228355	None:intergenic	25.0%
!!!	GATTCTCTCTTTTTTTTTCA+TGG	-	chr3_3:42228590-42228609	Msa0452810:three_prime_UTR	25.0%
!	CCTACTATTACCAAATTTAA+TGG	+	chr3_3:42228903-42228922	None:intergenic	25.0%
!!	CCATTAAATTTGGTAATAGT+AGG	-	chr3_3:42228900-42228919	Msa0452810:intron	25.0%
!	GGATTTCGTATAGTAATTAA+TGG	-	chr3_3:42229217-42229236	Msa0452810:intron	25.0%
!	CGTATAGTAATTAATGGAAA+AGG	-	chr3_3:42229223-42229242	Msa0452810:CDS	25.0%
!	ATAGTAATTAATGGAAAAGG+AGG	-	chr3_3:42229226-42229245	Msa0452810:CDS	25.0%
!	TAGTAATTAATGGAAAAGGA+GGG	-	chr3_3:42229227-42229246	Msa0452810:CDS	25.0%
!	TTACATGTACATCATTTGAA+TGG	+	chr3_3:42229374-42229393	None:intergenic	25.0%
!	TATGGTTAACGTCATTAATT+TGG	-	chr3_3:42229822-42229841	Msa0452810:five_prime_UTR	25.0%
!	TTCCAAAAAGGTAATAACTT+TGG	+	chr3_3:42228336-42228355	None:intergenic	25.0%
!!!	GATTCTCTCTTTTTTTTTCA+TGG	-	chr3_3:42228590-42228609	Msa0452810:three_prime_UTR	25.0%
!	CCTACTATTACCAAATTTAA+TGG	+	chr3_3:42228903-42228922	None:intergenic	25.0%
!!	CCATTAAATTTGGTAATAGT+AGG	-	chr3_3:42228900-42228919	Msa0452810:intron	25.0%
!	GGATTTCGTATAGTAATTAA+TGG	-	chr3_3:42229217-42229236	Msa0452810:intron	25.0%
!	CGTATAGTAATTAATGGAAA+AGG	-	chr3_3:42229223-42229242	Msa0452810:CDS	25.0%
!	ATAGTAATTAATGGAAAAGG+AGG	-	chr3_3:42229226-42229245	Msa0452810:CDS	25.0%
!	TAGTAATTAATGGAAAAGGA+GGG	-	chr3_3:42229227-42229246	Msa0452810:CDS	25.0%
!	TTACATGTACATCATTTGAA+TGG	+	chr3_3:42229374-42229393	None:intergenic	25.0%
!	TATGGTTAACGTCATTAATT+TGG	-	chr3_3:42229822-42229841	Msa0452810:five_prime_UTR	25.0%
	ACTTTGGCGATGTATATATA+GGG	+	chr3_3:42228320-42228339	None:intergenic	30.0%
	AACTTTGGCGATGTATATAT+AGG	+	chr3_3:42228321-42228340	None:intergenic	30.0%
	AACATGCTGTTATTCAATTC+CGG	-	chr3_3:42228407-42228426	Msa0452810:three_prime_UTR	30.0%
	TCGCTAAATTAGAGAAATAC+CGG	+	chr3_3:42228429-42228448	None:intergenic	30.0%
!	TCTTATTAGGCAAGTTGAAA+AGG	-	chr3_3:42228524-42228543	Msa0452810:three_prime_UTR	30.0%
	GTATGATAGTAAAGCAAACA+AGG	-	chr3_3:42228559-42228578	Msa0452810:three_prime_UTR	30.0%
!	GGTTTTACATGCAGAAAATA+TGG	+	chr3_3:42228634-42228653	None:intergenic	30.0%
	AAAACATTCGTTAATGTGAG+TGG	-	chr3_3:42228710-42228729	Msa0452810:intron	30.0%
!!!	ATCTGATACTTTGTTTTTGC+AGG	-	chr3_3:42228829-42228848	Msa0452810:intron	30.0%
	TCAACGGTATTATGAACTTA+GGG	-	chr3_3:42228868-42228887	Msa0452810:intron	30.0%
	TCCTCAATCTAAGTACTTAA+AGG	+	chr3_3:42229118-42229137	None:intergenic	30.0%
	AATGGTAAACTCATTTCTGT+AGG	+	chr3_3:42229356-42229375	None:intergenic	30.0%
	GACTTGATTAGAAAAACTAC+TGG	+	chr3_3:42229581-42229600	None:intergenic	30.0%
!	CTACAATCTTTCTGAAAAGA+GGG	+	chr3_3:42229619-42229638	None:intergenic	30.0%
	TTTCCTTCCATATCTTCAAA+GGG	+	chr3_3:42229725-42229744	None:intergenic	30.0%
	TTTGAAGATATGGAAGGAAA+GGG	-	chr3_3:42229725-42229744	Msa0452810:five_prime_UTR	30.0%
	ACTTTGGCGATGTATATATA+GGG	+	chr3_3:42228320-42228339	None:intergenic	30.0%
	AACTTTGGCGATGTATATAT+AGG	+	chr3_3:42228321-42228340	None:intergenic	30.0%
	AACATGCTGTTATTCAATTC+CGG	-	chr3_3:42228407-42228426	Msa0452810:three_prime_UTR	30.0%
	TCGCTAAATTAGAGAAATAC+CGG	+	chr3_3:42228429-42228448	None:intergenic	30.0%
!	TCTTATTAGGCAAGTTGAAA+AGG	-	chr3_3:42228524-42228543	Msa0452810:three_prime_UTR	30.0%
	GTATGATAGTAAAGCAAACA+AGG	-	chr3_3:42228559-42228578	Msa0452810:three_prime_UTR	30.0%
!	GGTTTTACATGCAGAAAATA+TGG	+	chr3_3:42228634-42228653	None:intergenic	30.0%
	AAAACATTCGTTAATGTGAG+TGG	-	chr3_3:42228710-42228729	Msa0452810:intron	30.0%
!!!	ATCTGATACTTTGTTTTTGC+AGG	-	chr3_3:42228829-42228848	Msa0452810:intron	30.0%
	TCAACGGTATTATGAACTTA+GGG	-	chr3_3:42228868-42228887	Msa0452810:intron	30.0%
	TCCTCAATCTAAGTACTTAA+AGG	+	chr3_3:42229118-42229137	None:intergenic	30.0%
	AATGGTAAACTCATTTCTGT+AGG	+	chr3_3:42229356-42229375	None:intergenic	30.0%
	GACTTGATTAGAAAAACTAC+TGG	+	chr3_3:42229581-42229600	None:intergenic	30.0%
!	CTACAATCTTTCTGAAAAGA+GGG	+	chr3_3:42229619-42229638	None:intergenic	30.0%
	TTTCCTTCCATATCTTCAAA+GGG	+	chr3_3:42229725-42229744	None:intergenic	30.0%
	TTTGAAGATATGGAAGGAAA+GGG	-	chr3_3:42229725-42229744	Msa0452810:five_prime_UTR	30.0%
	CTTTGGCGATGTATATATAG+GGG	+	chr3_3:42228319-42228338	None:intergenic	35.0%
!	CGCCAAAGTTATTACCTTTT+TGG	-	chr3_3:42228331-42228350	Msa0452810:three_prime_UTR	35.0%
!!	ACCTTTTTGGAAGAGAAAGA+AGG	-	chr3_3:42228344-42228363	Msa0452810:three_prime_UTR	35.0%
!!	CCTTTTTGGAAGAGAAAGAA+GGG	-	chr3_3:42228345-42228364	Msa0452810:three_prime_UTR	35.0%
	TGCTTTACTATCATACCATC+AGG	+	chr3_3:42228555-42228574	None:intergenic	35.0%
	TGCAGATCACAAAGATGTTA+GGG	+	chr3_3:42228655-42228674	None:intergenic	35.0%
	TTGCAGATCACAAAGATGTT+AGG	+	chr3_3:42228656-42228675	None:intergenic	35.0%
	ACATCTTTGTGATCTGCAAT+AGG	-	chr3_3:42228657-42228676	Msa0452810:intron	35.0%
!	AGCAAATAGTGAGTTGAAGA+TGG	-	chr3_3:42228790-42228809	Msa0452810:intron	35.0%
	TAATACCGTTGAGGTTCAAT+GGG	+	chr3_3:42228860-42228879	None:intergenic	35.0%
	ATAATACCGTTGAGGTTCAA+TGG	+	chr3_3:42228861-42228880	None:intergenic	35.0%
	CTAAGTTCATAATACCGTTG+AGG	+	chr3_3:42228869-42228888	None:intergenic	35.0%
	CTCAACGGTATTATGAACTT+AGG	-	chr3_3:42228867-42228886	Msa0452810:intron	35.0%
	GCCTTTAAGTACTTAGATTG+AGG	-	chr3_3:42229114-42229133	Msa0452810:intron	35.0%
!!	GGAAAAGAGTTGATGTTATC+AGG	-	chr3_3:42229135-42229154	Msa0452810:intron	35.0%
	CAGAACAAGATATAGTTCCA+TGG	+	chr3_3:42229332-42229351	None:intergenic	35.0%
	CATGGAACTATATCTTGTTC+TGG	-	chr3_3:42229330-42229349	Msa0452810:intron	35.0%
	CGTGCAGTAATACATATTCT+CGG	+	chr3_3:42229471-42229490	None:intergenic	35.0%
	AAGAGGGAATACACAAAACT+TGG	+	chr3_3:42229603-42229622	None:intergenic	35.0%
!	CCTACAATCTTTCTGAAAAG+AGG	+	chr3_3:42229620-42229639	None:intergenic	35.0%
!	CCTCTTTTCAGAAAGATTGT+AGG	-	chr3_3:42229617-42229636	Msa0452810:intron	35.0%
	TTCCTTCCATATCTTCAAAG+GGG	+	chr3_3:42229724-42229743	None:intergenic	35.0%
	CTTTCCTTCCATATCTTCAA+AGG	+	chr3_3:42229726-42229745	None:intergenic	35.0%
!	CTTTGAAGATATGGAAGGAA+AGG	-	chr3_3:42229724-42229743	Msa0452810:five_prime_UTR	35.0%
	CTTTGGCGATGTATATATAG+GGG	+	chr3_3:42228319-42228338	None:intergenic	35.0%
!	CGCCAAAGTTATTACCTTTT+TGG	-	chr3_3:42228331-42228350	Msa0452810:three_prime_UTR	35.0%
!!	ACCTTTTTGGAAGAGAAAGA+AGG	-	chr3_3:42228344-42228363	Msa0452810:three_prime_UTR	35.0%
!!	CCTTTTTGGAAGAGAAAGAA+GGG	-	chr3_3:42228345-42228364	Msa0452810:three_prime_UTR	35.0%
	TGCTTTACTATCATACCATC+AGG	+	chr3_3:42228555-42228574	None:intergenic	35.0%
	TGCAGATCACAAAGATGTTA+GGG	+	chr3_3:42228655-42228674	None:intergenic	35.0%
	TTGCAGATCACAAAGATGTT+AGG	+	chr3_3:42228656-42228675	None:intergenic	35.0%
	ACATCTTTGTGATCTGCAAT+AGG	-	chr3_3:42228657-42228676	Msa0452810:intron	35.0%
!	AGCAAATAGTGAGTTGAAGA+TGG	-	chr3_3:42228790-42228809	Msa0452810:intron	35.0%
	TAATACCGTTGAGGTTCAAT+GGG	+	chr3_3:42228860-42228879	None:intergenic	35.0%
	ATAATACCGTTGAGGTTCAA+TGG	+	chr3_3:42228861-42228880	None:intergenic	35.0%
	CTAAGTTCATAATACCGTTG+AGG	+	chr3_3:42228869-42228888	None:intergenic	35.0%
	CTCAACGGTATTATGAACTT+AGG	-	chr3_3:42228867-42228886	Msa0452810:intron	35.0%
	GCCTTTAAGTACTTAGATTG+AGG	-	chr3_3:42229114-42229133	Msa0452810:intron	35.0%
!!	GGAAAAGAGTTGATGTTATC+AGG	-	chr3_3:42229135-42229154	Msa0452810:intron	35.0%
	CAGAACAAGATATAGTTCCA+TGG	+	chr3_3:42229332-42229351	None:intergenic	35.0%
	CATGGAACTATATCTTGTTC+TGG	-	chr3_3:42229330-42229349	Msa0452810:intron	35.0%
	CGTGCAGTAATACATATTCT+CGG	+	chr3_3:42229471-42229490	None:intergenic	35.0%
	AAGAGGGAATACACAAAACT+TGG	+	chr3_3:42229603-42229622	None:intergenic	35.0%
!	CCTACAATCTTTCTGAAAAG+AGG	+	chr3_3:42229620-42229639	None:intergenic	35.0%
!	CCTCTTTTCAGAAAGATTGT+AGG	-	chr3_3:42229617-42229636	Msa0452810:intron	35.0%
	TTCCTTCCATATCTTCAAAG+GGG	+	chr3_3:42229724-42229743	None:intergenic	35.0%
	CTTTCCTTCCATATCTTCAA+AGG	+	chr3_3:42229726-42229745	None:intergenic	35.0%
!	CTTTGAAGATATGGAAGGAA+AGG	-	chr3_3:42229724-42229743	Msa0452810:five_prime_UTR	35.0%
	CCCTTCTTTCTCTTCCAAAA+AGG	+	chr3_3:42228348-42228367	None:intergenic	40.0%
	TGCAAGATGGATGATCTGTT+GGG	+	chr3_3:42228381-42228400	None:intergenic	40.0%
	TCTCTAATTTAGCGACTCCA+TGG	-	chr3_3:42228434-42228453	Msa0452810:three_prime_UTR	40.0%
	CTCTAATTTAGCGACTCCAT+GGG	-	chr3_3:42228435-42228454	Msa0452810:three_prime_UTR	40.0%
	TCTAATTTAGCGACTCCATG+GGG	-	chr3_3:42228436-42228455	Msa0452810:three_prime_UTR	40.0%
	CTGCAATAGGCCTTACATAA+TGG	-	chr3_3:42228670-42228689	Msa0452810:intron	40.0%
!!!	ATGTTTTGTTTGTGCTTCCG+AGG	+	chr3_3:42228697-42228716	None:intergenic	40.0%
	ATAGTCCCATTGAACCTCAA+CGG	-	chr3_3:42228852-42228871	Msa0452810:intron	40.0%
	GTATTATGAACTTAGGGAGC+AGG	-	chr3_3:42228874-42228893	Msa0452810:intron	40.0%
	GAGCAGGACACCATTAAATT+TGG	-	chr3_3:42228890-42228909	Msa0452810:intron	40.0%
	TAATGGAAAAGGAGGGTCTT+TGG	-	chr3_3:42229234-42229253	Msa0452810:CDS	40.0%
	AATGGAAAAGGAGGGTCTTT+GGG	-	chr3_3:42229235-42229254	Msa0452810:CDS	40.0%
	GGTGGAAAAGGAGAATCTAT+AGG	-	chr3_3:42229256-42229275	Msa0452810:CDS	40.0%
	GGAAAAGGAGAATCTATAGG+AGG	-	chr3_3:42229259-42229278	Msa0452810:CDS	40.0%
	AAAGGAGAATCTATAGGAGG+AGG	-	chr3_3:42229262-42229281	Msa0452810:CDS	40.0%
	AGAATCTATAGGAGGAGGAA+AGG	-	chr3_3:42229267-42229286	Msa0452810:CDS	40.0%
	TTTGATCACTTCTGATGAGC+AGG	+	chr3_3:42229547-42229566	None:intergenic	40.0%
!	AAGGCTCTGACATTCTCATT+TGG	+	chr3_3:42229686-42229705	None:intergenic	40.0%
!	GAAATGCCCCTTTGAAGATA+TGG	-	chr3_3:42229715-42229734	Msa0452810:five_prime_UTR	40.0%
	CCCTTCTTTCTCTTCCAAAA+AGG	+	chr3_3:42228348-42228367	None:intergenic	40.0%
	TGCAAGATGGATGATCTGTT+GGG	+	chr3_3:42228381-42228400	None:intergenic	40.0%
	TCTCTAATTTAGCGACTCCA+TGG	-	chr3_3:42228434-42228453	Msa0452810:three_prime_UTR	40.0%
	CTCTAATTTAGCGACTCCAT+GGG	-	chr3_3:42228435-42228454	Msa0452810:three_prime_UTR	40.0%
	TCTAATTTAGCGACTCCATG+GGG	-	chr3_3:42228436-42228455	Msa0452810:three_prime_UTR	40.0%
	CTGCAATAGGCCTTACATAA+TGG	-	chr3_3:42228670-42228689	Msa0452810:intron	40.0%
!!!	ATGTTTTGTTTGTGCTTCCG+AGG	+	chr3_3:42228697-42228716	None:intergenic	40.0%
	ATAGTCCCATTGAACCTCAA+CGG	-	chr3_3:42228852-42228871	Msa0452810:intron	40.0%
	GTATTATGAACTTAGGGAGC+AGG	-	chr3_3:42228874-42228893	Msa0452810:intron	40.0%
	GAGCAGGACACCATTAAATT+TGG	-	chr3_3:42228890-42228909	Msa0452810:intron	40.0%
	TAATGGAAAAGGAGGGTCTT+TGG	-	chr3_3:42229234-42229253	Msa0452810:CDS	40.0%
	AATGGAAAAGGAGGGTCTTT+GGG	-	chr3_3:42229235-42229254	Msa0452810:CDS	40.0%
	GGTGGAAAAGGAGAATCTAT+AGG	-	chr3_3:42229256-42229275	Msa0452810:CDS	40.0%
	GGAAAAGGAGAATCTATAGG+AGG	-	chr3_3:42229259-42229278	Msa0452810:CDS	40.0%
	AAAGGAGAATCTATAGGAGG+AGG	-	chr3_3:42229262-42229281	Msa0452810:CDS	40.0%
	AGAATCTATAGGAGGAGGAA+AGG	-	chr3_3:42229267-42229286	Msa0452810:CDS	40.0%
	TTTGATCACTTCTGATGAGC+AGG	+	chr3_3:42229547-42229566	None:intergenic	40.0%
!	AAGGCTCTGACATTCTCATT+TGG	+	chr3_3:42229686-42229705	None:intergenic	40.0%
!	GAAATGCCCCTTTGAAGATA+TGG	-	chr3_3:42229715-42229734	Msa0452810:five_prime_UTR	40.0%
	CTGCAAGATGGATGATCTGT+TGG	+	chr3_3:42228382-42228401	None:intergenic	45.0%
	CTAATTTAGCGACTCCATGG+GGG	-	chr3_3:42228437-42228456	Msa0452810:three_prime_UTR	45.0%
	CAAACAAACAGAAACCCCCA+TGG	+	chr3_3:42228454-42228473	None:intergenic	45.0%
	GTTGAAAAGGAGCAACCTGA+TGG	-	chr3_3:42228537-42228556	Msa0452810:three_prime_UTR	45.0%
!!	AGGCCTTACATAATGGACCT+CGG	-	chr3_3:42228677-42228696	Msa0452810:intron	45.0%
	CTTCCGAGGTCCATTATGTA+AGG	+	chr3_3:42228683-42228702	None:intergenic	45.0%
!	GGCTTTTGCTACAGCATCTT+AGG	+	chr3_3:42229097-42229116	None:intergenic	45.0%
	AGGATGGTACAGATTTGGAG+AGG	+	chr3_3:42229527-42229546	None:intergenic	45.0%
	TGAGCAGGATGGTACAGATT+TGG	+	chr3_3:42229532-42229551	None:intergenic	45.0%
	ATCACTTCTGATGAGCAGGA+TGG	+	chr3_3:42229543-42229562	None:intergenic	45.0%
!	TGCCCCTTTGAAGATATGGA+AGG	-	chr3_3:42229719-42229738	Msa0452810:five_prime_UTR	45.0%
	CTGCAAGATGGATGATCTGT+TGG	+	chr3_3:42228382-42228401	None:intergenic	45.0%
	CTAATTTAGCGACTCCATGG+GGG	-	chr3_3:42228437-42228456	Msa0452810:three_prime_UTR	45.0%
	CAAACAAACAGAAACCCCCA+TGG	+	chr3_3:42228454-42228473	None:intergenic	45.0%
	GTTGAAAAGGAGCAACCTGA+TGG	-	chr3_3:42228537-42228556	Msa0452810:three_prime_UTR	45.0%
!!	AGGCCTTACATAATGGACCT+CGG	-	chr3_3:42228677-42228696	Msa0452810:intron	45.0%
	CTTCCGAGGTCCATTATGTA+AGG	+	chr3_3:42228683-42228702	None:intergenic	45.0%
!	GGCTTTTGCTACAGCATCTT+AGG	+	chr3_3:42229097-42229116	None:intergenic	45.0%
	AGGATGGTACAGATTTGGAG+AGG	+	chr3_3:42229527-42229546	None:intergenic	45.0%
	TGAGCAGGATGGTACAGATT+TGG	+	chr3_3:42229532-42229551	None:intergenic	45.0%
	ATCACTTCTGATGAGCAGGA+TGG	+	chr3_3:42229543-42229562	None:intergenic	45.0%
!	TGCCCCTTTGAAGATATGGA+AGG	-	chr3_3:42229719-42229738	Msa0452810:five_prime_UTR	45.0%
!!!	ATTAAATTTATTTTCTTATT+AGG	-	chr3_3:42228511-42228530	Msa0452810:three_prime_UTR	5.0%
!!!	TTTGATTTTAAAATAATTTT+AGG	-	chr3_3:42228977-42228996	Msa0452810:intron	5.0%
!!	TAAATAATATACATTTATAT+TGG	+	chr3_3:42229419-42229438	None:intergenic	5.0%
!!!	AAAAAAAAAAACATATTTTA+TGG	-	chr3_3:42229804-42229823	Msa0452810:five_prime_UTR	5.0%
!!!	ATTAAATTTATTTTCTTATT+AGG	-	chr3_3:42228511-42228530	Msa0452810:three_prime_UTR	5.0%
!!!	TTTGATTTTAAAATAATTTT+AGG	-	chr3_3:42228977-42228996	Msa0452810:intron	5.0%
!!	TAAATAATATACATTTATAT+TGG	+	chr3_3:42229419-42229438	None:intergenic	5.0%
!!!	AAAAAAAAAAACATATTTTA+TGG	-	chr3_3:42229804-42229823	Msa0452810:five_prime_UTR	5.0%
!	GCATGTTGCTTGCTGCAAGA+TGG	+	chr3_3:42228394-42228413	None:intergenic	50.0%
!	GGGGCATTTCGCTTAAGCAA+AGG	+	chr3_3:42229705-42229724	None:intergenic	50.0%
!	GCATGTTGCTTGCTGCAAGA+TGG	+	chr3_3:42228394-42228413	None:intergenic	50.0%
!	GGGGCATTTCGCTTAAGCAA+AGG	+	chr3_3:42229705-42229724	None:intergenic	50.0%
	GGAAAAGGAGGGTCTTTGGG+TGG	-	chr3_3:42229238-42229257	Msa0452810:CDS	55.0%
	GGAGGGTCTTTGGGTGGAAA+AGG	-	chr3_3:42229244-42229263	Msa0452810:CDS	55.0%
	GGAAAAGGAGGGTCTTTGGG+TGG	-	chr3_3:42229238-42229257	Msa0452810:CDS	55.0%
	GGAGGGTCTTTGGGTGGAAA+AGG	-	chr3_3:42229244-42229263	Msa0452810:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	42228293	42229847	42228293	ID=Msa0452810;Name=Msa0452810
chr3_3	mRNA	42228293	42229847	42228293	ID=Msa0452810-mRNA-1;Parent=Msa0452810;Name=Msa0452810-mRNA-1;_AED=0.10;_eAED=0.10;_QI=164\|0.75\|0.8\|1\|0.75\|0.8\|5\|334\|60
chr3_3	exon	42228293	42228672	42228293	ID=Msa0452810-mRNA-1:exon:7693;Parent=Msa0452810-mRNA-1
chr3_3	exon	42229219	42229289	42229219	ID=Msa0452810-mRNA-1:exon:7692;Parent=Msa0452810-mRNA-1
chr3_3	exon	42229416	42229461	42229416	ID=Msa0452810-mRNA-1:exon:7691;Parent=Msa0452810-mRNA-1
chr3_3	exon	42229560	42229607	42229560	ID=Msa0452810-mRNA-1:exon:7690;Parent=Msa0452810-mRNA-1
chr3_3	exon	42229712	42229847	42229712	ID=Msa0452810-mRNA-1:exon:7689;Parent=Msa0452810-mRNA-1
chr3_3	five_prime_UTR	42229712	42229847	42229712	ID=Msa0452810-mRNA-1:five_prime_utr;Parent=Msa0452810-mRNA-1
chr3_3	five_prime_UTR	42229580	42229607	42229580	ID=Msa0452810-mRNA-1:five_prime_utr;Parent=Msa0452810-mRNA-1
chr3_3	CDS	42229560	42229579	42229560	ID=Msa0452810-mRNA-1:cds;Parent=Msa0452810-mRNA-1
chr3_3	CDS	42229416	42229461	42229416	ID=Msa0452810-mRNA-1:cds;Parent=Msa0452810-mRNA-1
chr3_3	CDS	42229219	42229289	42229219	ID=Msa0452810-mRNA-1:cds;Parent=Msa0452810-mRNA-1
chr3_3	CDS	42228627	42228672	42228627	ID=Msa0452810-mRNA-1:cds;Parent=Msa0452810-mRNA-1
chr3_3	three_prime_UTR	42228293	42228626	42228293	ID=Msa0452810-mRNA-1:three_prime_utr;Parent=Msa0452810-mRNA-1

Gene Sequence	>Msa0452810 ATGATAGTAAAGCAAACAAGGCCTTACATAATGGACCTCGGAAGCACAAACAAAACATTCGTTAATGATAGTCCCATTGAACCTCAACGGTATTATGAACTTAGGGAGCAGGACACCATTAAATTTGGTAATAGTAGCCGAGAATATGTATTACTGCACGAGAACTCTGCTTCTGCTTCGTGA
Protein sequence	>Msa0452810 MIVKQTRPYIMDLGSTNKTFVNDSPIEPQRYYELREQDTIKFGNSSREYVLLHENSASAS*