AlfalfaGEDB | Msa0099730

M. sativa cultivar zhongmu-4 / Msa0099730

KEGG: -
GO: -
PFAM: PF07911.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099730	XP_013465872.1	98.658	149	2	0	80	228	1	149	2.19e-102	303

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099730	A0A072VER7	98.658	149	2	0	80	228	1	149	1.04e-102	303

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0099730	-0.825962	3.521098e-54	-8.615850e-47
Msa0005350	Msa0099730	0.997390	1.089440e-241	-8.615850e-47
Msa0006700	Msa0099730	0.822425	2.385164e-53	-8.615850e-47
Msa0008850	Msa0099730	0.818110	2.325615e-52	-8.615850e-47
Msa0011720	Msa0099730	0.824162	9.370668e-54	-8.615850e-47
Msa0017120	Msa0099730	0.804702	1.903151e-49	-8.615850e-47
Msa0030030	Msa0099730	0.810518	1.107504e-50	-8.615850e-47
Msa0047470	Msa0099730	0.822854	1.895482e-53	-8.615850e-47
Msa0050730	Msa0099730	0.821558	3.788827e-53	-8.615850e-47
Msa0050760	Msa0099730	0.818691	1.717362e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099730	MtrunA17_Chr1g0150711	98.658	149	2	0	80	228	1	149	2.01e-106	303
Msa0099730	MtrunA17_Chr3g0136441	54.839	124	45	4	92	214	11	124	7.04e-36	123
Msa0099730	MtrunA17_Chr4g0070351	50.862	116	49	3	79	192	17	126	2.40e-29	106
Msa0099730	MtrunA17_Chr8g0344381	46.400	125	54	5	99	215	34	153	4.39e-29	107
Msa0099730	MtrunA17_Chr4g0070371	45.082	122	58	3	74	192	6	121	2.61e-27	102
Msa0099730	MtrunA17_Chr3g0140381	36.538	104	62	2	92	194	33	133	4.03e-17	75.5
Msa0099730	MtrunA17_Chr1g0205251	43.836	73	39	1	96	168	3	73	8.04e-17	73.9
Msa0099730	MtrunA17_Chr5g0394601	39.560	91	49	2	96	186	3	87	1.02e-16	73.2
Msa0099730	MtrunA17_Chr8g0340421	30.108	186	100	8	47	215	11	183	1.68e-16	74.7
Msa0099730	MtrunA17_Chr2g0331111	50.794	63	27	1	96	158	63	121	7.89e-15	70.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099730	AT1G72510.1	43.939	132	57	5	98	215	36	164	1.43e-28	106
Msa0099730	AT1G72510.2	43.939	132	57	5	98	215	36	164	1.43e-28	106
Msa0099730	AT5G20670.1	43.478	138	63	3	90	215	17	151	4.70e-27	102
Msa0099730	AT2G09970.1	37.931	145	71	6	85	215	26	165	5.31e-23	91.7
Msa0099730	AT1G54095.1	44.037	109	59	2	80	188	1	107	1.98e-19	82.0
Msa0099730	AT3G22540.1	37.500	120	59	5	92	210	2	106	6.10e-18	77.0
Msa0099730	AT4G14819.1	36.134	119	59	4	92	210	2	103	1.09e-16	73.6
Msa0099730	AT2G25780.1	35.537	121	70	4	99	217	39	153	2.68e-13	65.9
Msa0099730	AT1G79770.1	70.000	40	12	0	96	135	52	91	4.09e-13	65.5
Msa0099730	AT5G25840.1	36.923	130	62	6	96	215	43	162	1.36e-12	64.3

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Find 55 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCAGCTGTTGCGGGT+TGG	0.633011	1_3:+5940346	Msa0099730:CDS
AAGCAGAAGATCTGAACTGC+TGG	0.512505	1_3:-5940535	None:intergenic
ACCGCAAAATGCAGCTGTTG+CGG	0.482069	1_3:+5940341	Msa0099730:CDS
ACGCGTACATTGCGCGCGTG+AGG	0.548939	1_3:+5940384	Msa0099730:CDS
AGAACGAACGATAATACATA+CGG	0.628567	1_3:+5939377	Msa0099730:CDS
AGAAGCTGAAGCGACAGAGA+TGG	0.523368	1_3:+5940307	Msa0099730:CDS
AGGCAAGCATTGTTGGTTGG+TGG	0.556701	1_3:+5940768	Msa0099730:three_prime_UTR
ATCGTTCGTTCTTCTCCCAC+TGG	0.415717	1_3:-5939366	None:intergenic
ATGACATTACAGAGCCGAAT+TGG	0.419760	1_3:-5939294	None:intergenic
ATGCAGCTGTTGCGGGTTGG+TGG	0.517846	1_3:+5940349	Msa0099730:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAACGAACGATAATACATA+CGG	+	chr1_3:5939377-5939396	Msa0099730:CDS	30.0%
TTCAAAATAATTTGATGCCG+TGG	+	chr1_3:5940008-5940027	Msa0099730:intron	30.0%
TTGTTTGTGCTTAAATTCAG+AGG	+	chr1_3:5940246-5940265	Msa0099730:intron	30.0%
TGCTAGTAATCAAAATGAGA+TGG	-	chr1_3:5940966-5940985	None:intergenic	30.0%
AGAACGAACGATAATACATA+CGG	+	chr1_3:5939377-5939396	Msa0099730:CDS	30.0%
TTCAAAATAATTTGATGCCG+TGG	+	chr1_3:5940008-5940027	Msa0099730:intron	30.0%
TTGTTTGTGCTTAAATTCAG+AGG	+	chr1_3:5940246-5940265	Msa0099730:intron	30.0%
TGCTAGTAATCAAAATGAGA+TGG	-	chr1_3:5940966-5940985	None:intergenic	30.0%
TTCAAATTCAAGAGATCCAG+TGG	+	chr1_3:5939350-5939369	Msa0099730:CDS	35.0%
TCAAATTCAAGAGATCCAGT+GGG	+	chr1_3:5939351-5939370	Msa0099730:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	5939183	5941011	5939183	ID=Msa0099730;Name=Msa0099730
chr1_3	mRNA	5939183	5941011	5939183	ID=Msa0099730-mRNA-1;Parent=Msa0099730;Name=Msa0099730-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|1\|1\|1\|0\|0.5\|2\|275\|228
chr1_3	exon	5939183	5939398	5939183	ID=Msa0099730-mRNA-1:exon:2259;Parent=Msa0099730-mRNA-1
chr1_3	exon	5940266	5941011	5940266	ID=Msa0099730-mRNA-1:exon:2260;Parent=Msa0099730-mRNA-1
chr1_3	CDS	5939183	5939398	5939183	ID=Msa0099730-mRNA-1:cds;Parent=Msa0099730-mRNA-1
chr1_3	CDS	5940266	5940736	5940266	ID=Msa0099730-mRNA-1:cds;Parent=Msa0099730-mRNA-1
chr1_3	three_prime_UTR	5940737	5941011	5940737	ID=Msa0099730-mRNA-1:three_prime_utr;Parent=Msa0099730-mRNA-1

Gene Sequence

>Msa0099730

GTGCGTATAGGTTGGTTAGTTTATATATCTATCTATCTGCCTCTGTTTTCGCATTCATCATCGTATCACTTCAATTCAACAACACACAGAAGAGAATCATCCATCGTAGAGCCAATTCGGCTCTGTAATGTCATTTGCTTTACTTTTACTTTTTGGCGGCCAAATACTTCAAATTCAAGAGATCCAGTGGGAGAAGAACGAACGATAATACATACGAGGAATCAAAGAAGTGTGCATATGGCTGCGAATGTTGTGAAAGAAGCTGAAGCGACAGAGATGGAAGCTGTTTTGACCGCAAAATGCAGCTGTTGCGGGTTGGTGGAAGAGTGCACACACGCGTACATTGCGCGCGTGAGGGAGAGATTCGGTGGAAGATGGATATGCGGTTTATGTGCTGAGGCAGTGAAGGAAGAAAGGGCGAGGTCGGAGAGTGATGAGAAGATGATCACAATTGATGAAGCTTTAATGCGGCACACCAAATTTCGCCAGCAGTTCAGATCTTCTGCTTCTGATCATAACAAAGATTTCATAGTTGCTGTGAAACAGATTCTTTTTAGGACTTTGGATTCTCCTAGGAGCAAGCCGAGTAAGGAACATTCTGCGTGCAGACCTCTTGGTAGATCTCATAGTTGCTTCTCAACAATGGAAACAACTCCGCCAAGGACGACAGAGACACATACAGAGTGA

Protein sequence

>Msa0099730

VRIGWLVYISIYLPLFSHSSSYHFNSTTHRRESSIVEPIRLCNVICFTFTFWRPNTSNSRDPVGEERTIIHTRNQRSVHMAANVVKEAEATEMEAVLTAKCSCCGLVEECTHAYIARVRERFGGRWICGLCAEAVKEERARSESDEKMITIDEALMRHTKFRQQFRSSASDHNKDFIVAVKQILFRTLDSPRSKPSKEHSACRPLGRSHSCFSTMETTPPRTTETHTE*