AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0757010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0757010 MtrunA17_Chr5g0402271 98.837 172 2 0 1 172 1 172 5.78e-124 346
Msa0757010 MtrunA17_Chr1g0190591 48.718 117 44 4 61 172 80 185 3.44e-24 93.2
Msa0757010 MtrunA17_Chr4g0044791 52.747 91 33 3 27 110 12 99 2.26e-23 90.1
Msa0757010 MtrunA17_Chr7g0274251 42.500 120 50 4 61 172 77 185 1.83e-19 80.9
Msa0757010 MtrunA17_Chr1g0213571 38.793 116 34 4 57 170 33 113 1.66e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0757010 AT4G33985.1 52.381 147 54 4 29 171 14 148 1.62e-39 132
Msa0757010 AT3G50350.2 52.448 143 53 4 33 172 38 168 3.61e-37 126
Msa0757010 AT2G15590.2 46.358 151 58 6 29 172 11 145 6.48e-33 115
Msa0757010 AT3G50350.1 56.364 110 37 5 7 111 18 121 3.33e-28 103
Msa0757010 AT2G15590.1 55.056 89 28 4 29 110 11 94 7.57e-24 90.9
Msa0757010 AT1G08790.1 40.559 143 66 5 42 172 53 188 1.40e-19 81.6
Msa0757010 AT3G04700.1 39.456 147 69 3 42 172 48 190 2.48e-19 81.3
Msa0757010 AT2G31560.2 39.316 117 57 4 57 170 94 199 4.08e-17 75.5
Msa0757010 AT2G31560.1 39.316 117 57 4 57 170 94 199 4.08e-17 75.5
Msa0757010 AT2G31560.3 39.316 117 57 4 57 170 94 199 4.08e-17 75.5
Msa0757010 AT5G28690.1 39.333 150 65 6 42 172 49 191 5.31e-17 75.1
Msa0757010 AT5G28690.3 39.333 150 65 6 42 172 49 191 5.31e-17 75.1
Msa0757010 AT5G28690.2 39.333 150 65 6 42 172 49 191 5.31e-17 75.1
Msa0757010 AT1G05870.4 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT1G05870.12 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT1G05870.8 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT1G05870.1 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT1G05870.7 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT1G05870.2 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT1G05870.10 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT1G05870.5 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT1G05870.9 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT1G05870.3 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT1G05870.6 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT1G05870.11 38.983 118 57 4 57 170 80 186 6.25e-17 74.7
Msa0757010 AT2G15610.1 45.349 86 42 3 29 110 12 96 7.59e-17 73.6
Msa0757010 AT3G04710.3 34.595 185 100 4 1 169 8 187 1.08e-16 77.0
Msa0757010 AT2G43340.1 40.171 117 58 4 56 170 80 186 1.73e-16 73.6

Find 90 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGAGTGCAAAGATTTA+AGG 0.183003 5_2:-11662621 None:intergenic
CTCAAAGCTTGCATTGAATT+AGG 0.228374 5_2:+11662967 Msa0757010:CDS
GGAAGACGCTATGGCTTATT+AGG 0.235569 5_2:-11662695 None:intergenic
GTAGTAACGGCGGCGGATTT+AGG 0.271292 5_2:-11663102 None:intergenic
GCTTGCATTGAATTAGGTTT+TGG 0.295486 5_2:+11662973 Msa0757010:CDS
AACCTTGTCTTCACCGTTTG+TGG 0.303740 5_2:-11663477 None:intergenic
CTTTAGGTACCAAAACAATC+TGG 0.316908 5_2:-11663726 None:intergenic
ATAATAACATCAACTACTTT+AGG 0.332767 5_2:-11663742 None:intergenic
TAGTAACGGCGGCGGATTTA+GGG 0.354317 5_2:-11663101 None:intergenic
TAACCAAACCCAGATTGTTT+TGG 0.374396 5_2:+11663717 Msa0757010:three_prime_UTR
ATGATTATGCATGATGATTC+TGG 0.374745 5_2:+11663546 Msa0757010:three_prime_UTR
AGCCCTCGCACCGCCATCTT+CGG 0.378249 5_2:+11663177 Msa0757010:CDS
ACACATGATTGTAACTTGTA+TGG 0.382625 5_2:-11663639 None:intergenic
CGACGATCAGTTTCAACTTC+GGG 0.385163 5_2:-11663009 None:intergenic
AGTGATTATTGATGATTAAC+CGG 0.397578 5_2:+11663586 Msa0757010:three_prime_UTR
GGCTCGTGGCCGGTTCTGTT+TGG 0.413415 5_2:-11663687 None:intergenic
GTACCAAAACAATCTGGGTT+TGG 0.415014 5_2:-11663720 None:intergenic
AATATTACCGTTGCCGAAGA+TGG 0.416533 5_2:-11663190 None:intergenic
GGTGGAGATGGCGGCGCGTA+GGG 0.419051 5_2:-11662772 None:intergenic
CTACACAATGAATACCAAAA+AGG 0.420369 5_2:-11663975 None:intergenic
GGCTTCGTCGTAAAGGAAAC+TGG 0.420518 5_2:+11662899 Msa0757010:CDS
AGAAAGGAAGGTGGTGGAGA+TGG 0.426640 5_2:-11662784 None:intergenic
TCTGGGTTTGGTTAAACTAA+AGG 0.430998 5_2:-11663708 None:intergenic
AGAAGAGTGCAAAGATTTAA+GGG 0.444563 5_2:-11662620 None:intergenic
TGGTGGAGATGGCGGCGCGT+AGG 0.463791 5_2:-11662773 None:intergenic
TTGCCGAAGATGGCGGTGCG+AGG 0.483550 5_2:-11663180 None:intergenic
TGGAAGAGAAAGGAAGGTGG+TGG 0.489175 5_2:-11662790 None:intergenic
GCGTTGTTGTGCGGAGGTCG+AGG 0.489529 5_2:-11662736 None:intergenic
ATCTTTACCGTGACGAAGCG+TGG 0.490402 5_2:+11662878 Msa0757010:CDS
CGAGGGCTGCCACGAGGAGA+AGG 0.497711 5_2:-11663162 None:intergenic
TTTAGGTACCAAAACAATCT+GGG 0.503636 5_2:-11663725 None:intergenic
GAAGCGTGGCTTCGTCGTAA+AGG 0.505096 5_2:+11662892 Msa0757010:CDS
GTTAAATTAATAACCACGGT+TGG 0.505727 5_2:-11663617 None:intergenic
GTAAAGGAAACTGGAAGAAT+CGG 0.510681 5_2:+11662908 Msa0757010:CDS
GCCATCACGATCGGAGGCTG+CGG 0.520742 5_2:-11663136 None:intergenic
AGGGACCTCGCACGGTGCGT+TGG 0.523223 5_2:-11662716 None:intergenic
TGCCGAAGATGGCGGTGCGA+GGG 0.523267 5_2:-11663179 None:intergenic
GCTTCGTCACGGTAAAGATC+AGG 0.527383 5_2:-11662874 None:intergenic
CTCTCCTCAAGCAGCGATCG+TGG 0.533172 5_2:+11662848 Msa0757010:CDS
ATTTCACTACCAAACAGAAC+CGG 0.537637 5_2:+11663678 Msa0757010:three_prime_UTR
TGGTGGACGGTGGAAGAGAA+AGG 0.537926 5_2:-11662800 None:intergenic
ATCACGATCGGAGGCTGCGG+AGG 0.538111 5_2:-11663133 None:intergenic
GCATGATAAAGTTCAAGAGC+AGG 0.542994 5_2:-11663048 None:intergenic
TGGTATTCATTGTGTAGGTG+TGG 0.545424 5_2:+11663981 Msa0757010:three_prime_UTR
CGGTGCGTTGGAAGACGCTA+TGG 0.546409 5_2:-11662704 None:intergenic
GATTTAAGGGTGTGGTGAGG+TGG 0.547933 5_2:-11662607 None:intergenic
GGTGAGGTGGTAGATCAAGT+TGG 0.555238 5_2:-11662594 None:intergenic
ACGACGATCAGTTTCAACTT+CGG 0.557808 5_2:-11663010 None:intergenic
AGAAGGAGTGCCATCACGAT+CGG 0.561060 5_2:-11663145 None:intergenic
GTCTTCCAACGCACCGTGCG+AGG 0.563962 5_2:+11662711 Msa0757010:five_prime_UTR
TCGGAGGCTGCGGAGGAAGA+CGG 0.564577 5_2:-11663126 None:intergenic
ATTGATATGTTACTTGCTGT+CGG 0.564691 5_2:+11663763 Msa0757010:three_prime_UTR
GGACGGTGGAAGAGAAAGGA+AGG 0.564692 5_2:-11662796 None:intergenic
AATTCTTGCCTTAGCAGTGG+TGG 0.566176 5_2:-11662817 None:intergenic
CGCACCGCCATCTTCGGCAA+CGG 0.567388 5_2:+11663183 Msa0757010:CDS
TTAAACTAAAGGCTCGTGGC+CGG 0.568803 5_2:-11663697 None:intergenic
GGCACAGGTTGTTGCTTGTG+CGG 0.568971 5_2:+11663509 Msa0757010:CDS
ATGCATGATGATTCTGGTGA+TGG 0.576855 5_2:+11663552 Msa0757010:three_prime_UTR
AAGGTTGAGACAGTGGGCAC+AGG 0.577432 5_2:+11663494 Msa0757010:CDS
TGAAGATGATGATCCACAAA+CGG 0.579168 5_2:+11663464 Msa0757010:intron
CGTTGTTGTGCGGAGGTCGA+GGG 0.580667 5_2:-11662735 None:intergenic
TCCACCGTCCACCACTGCTA+AGG 0.580926 5_2:+11662809 Msa0757010:CDS
CGGTGGAAGAGAAAGGAAGG+TGG 0.588878 5_2:-11662793 None:intergenic
CACGATCGCTGCTTGAGGAG+AGG 0.590516 5_2:-11662847 None:intergenic
AAGGAAGGTGGTGGAGATGG+CGG 0.597909 5_2:-11662781 None:intergenic
ATCCACAAACGGTGAAGACA+AGG 0.599536 5_2:+11663475 Msa0757010:CDS
AGTGCAAAGATTTAAGGGTG+TGG 0.601712 5_2:-11662615 None:intergenic
CTTGCCTTAGCAGTGGTGGA+CGG 0.601893 5_2:-11662813 None:intergenic
TATGGTTAAATTAATAACCA+CGG 0.602109 5_2:-11663621 None:intergenic
GGAGGAAGACGGTGTAGTAA+CGG 0.604938 5_2:-11663115 None:intergenic
GGAGGTCGAGGGACCTCGCA+CGG 0.605935 5_2:-11662724 None:intergenic
ATTACCGTTGCCGAAGATGG+CGG 0.608342 5_2:-11663187 None:intergenic
TCCGCAGCCTCCGATCGTGA+TGG 0.608934 5_2:+11663135 Msa0757010:CDS
GACGAAGCCACGCTTCGTCA+CGG 0.613832 5_2:-11662885 None:intergenic
ATCGGAGCGTTGTTGTGCGG+AGG 0.613915 5_2:-11662742 None:intergenic
GCGACCACGATCGCTGCTTG+AGG 0.614305 5_2:-11662852 None:intergenic
GACATCGGAGCGTTGTTGTG+CGG 0.617234 5_2:-11662745 None:intergenic
GCCTTAGCAGTGGTGGACGG+TGG 0.621956 5_2:-11662810 None:intergenic
TGAAGACAAGGTTGAGACAG+TGG 0.625033 5_2:+11663487 Msa0757010:CDS
GAGAATTCTTGCCTTAGCAG+TGG 0.625843 5_2:-11662820 None:intergenic
AGACGGTGTAGTAACGGCGG+CGG 0.627507 5_2:-11663109 None:intergenic
TTGGTTAAACTAAAGGCTCG+TGG 0.638119 5_2:-11663701 None:intergenic
GGCGCGTAGGGATCAGACAT+CGG 0.645725 5_2:-11662760 None:intergenic
GATGGCACTCCTTCTCCTCG+TGG 0.649473 5_2:+11663153 Msa0757010:CDS
GAAGACAAGGTTGAGACAGT+GGG 0.660668 5_2:+11663488 Msa0757010:CDS
AGGAGTGCCATCACGATCGG+AGG 0.666999 5_2:-11663142 None:intergenic
TATTGTCTAAAATGTGACTG+TGG 0.705865 5_2:+11663814 Msa0757010:three_prime_UTR
GGAAGACGGTGTAGTAACGG+CGG 0.724547 5_2:-11663112 None:intergenic
GCGGTGCGAGGGCTGCCACG+AGG 0.734065 5_2:-11663168 None:intergenic
AAAGATTTAAGGGTGTGGTG+AGG 0.752540 5_2:-11662610 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAATTATAATGATTCT+CGG + chr5_2:11663076-11663095 Msa0757010:CDS 10.0%
!! ATAAAAATTATAATGATTCT+CGG + chr5_2:11663076-11663095 Msa0757010:CDS 10.0%
!!! TTTTTGTTTTGTTTTGGATT+CGG - chr5_2:11662664-11662683 None:intergenic 20.0%
!!! TTGGTTTTTTTGTTTTGTTT+TGG - chr5_2:11662670-11662689 None:intergenic 20.0%
!! TCATATTGAATTAAGATCTA+TGG + chr5_2:11663376-11663395 Msa0757010:intron 20.0%
!! TATGGTTAAATTAATAACCA+CGG - chr5_2:11663624-11663643 None:intergenic 20.0%
!! ATAATAACATCAACTACTTT+AGG - chr5_2:11663745-11663764 None:intergenic 20.0%
!!! TTTTTGTTTTGTTTTGGATT+CGG - chr5_2:11662664-11662683 None:intergenic 20.0%
!!! TTGGTTTTTTTGTTTTGTTT+TGG - chr5_2:11662670-11662689 None:intergenic 20.0%
!! TCATATTGAATTAAGATCTA+TGG + chr5_2:11663376-11663395 Msa0757010:intron 20.0%
!! TATGGTTAAATTAATAACCA+CGG - chr5_2:11663624-11663643 None:intergenic 20.0%
!! ATAATAACATCAACTACTTT+AGG - chr5_2:11663745-11663764 None:intergenic 20.0%
!!! TATGGCTTATTAGGTTTTTT+TGG - chr5_2:11662689-11662708 None:intergenic 25.0%
!! GTATTCTAAGAGTAATTAAG+AGG - chr5_2:11663283-11663302 None:intergenic 25.0%
!! AGTGATTATTGATGATTAAC+CGG + chr5_2:11663586-11663605 Msa0757010:three_prime_UTR 25.0%
! TTAAAAATGTTACTACTACG+TGG + chr5_2:11663933-11663952 Msa0757010:three_prime_UTR 25.0%
!!! TATGGCTTATTAGGTTTTTT+TGG - chr5_2:11662689-11662708 None:intergenic 25.0%
!! GTATTCTAAGAGTAATTAAG+AGG - chr5_2:11663283-11663302 None:intergenic 25.0%
!! AGTGATTATTGATGATTAAC+CGG + chr5_2:11663586-11663605 Msa0757010:three_prime_UTR 25.0%
! TTAAAAATGTTACTACTACG+TGG + chr5_2:11663933-11663952 Msa0757010:three_prime_UTR 25.0%
GACCAATAAAAAAGAGGTAA+GGG + chr5_2:11662568-11662587 Msa0757010:five_prime_UTR 30.0%
!!! AACCCTTACCTCTTTTTTAT+TGG - chr5_2:11662573-11662592 None:intergenic 30.0%
! AGAAGAGTGCAAAGATTTAA+GGG - chr5_2:11662623-11662642 None:intergenic 30.0%
! AAGAAGAGTGCAAAGATTTA+AGG - chr5_2:11662624-11662643 None:intergenic 30.0%
ATGATTATGCATGATGATTC+TGG + chr5_2:11663546-11663565 Msa0757010:three_prime_UTR 30.0%
GTTAAATTAATAACCACGGT+TGG - chr5_2:11663620-11663639 None:intergenic 30.0%
! ACACATGATTGTAACTTGTA+TGG - chr5_2:11663642-11663661 None:intergenic 30.0%
TTTAGGTACCAAAACAATCT+GGG - chr5_2:11663728-11663747 None:intergenic 30.0%
!! ATTGATATGTTACTTGCTGT+CGG + chr5_2:11663763-11663782 Msa0757010:three_prime_UTR 30.0%
! TATTGTCTAAAATGTGACTG+TGG + chr5_2:11663814-11663833 Msa0757010:three_prime_UTR 30.0%
! TTTTGTGTCAGAAAGAAAGA+CGG + chr5_2:11663903-11663922 Msa0757010:three_prime_UTR 30.0%
CTACACAATGAATACCAAAA+AGG - chr5_2:11663978-11663997 None:intergenic 30.0%
!! CTTTTTGGTATTCATTGTGT+AGG + chr5_2:11663976-11663995 Msa0757010:three_prime_UTR 30.0%
GACCAATAAAAAAGAGGTAA+GGG + chr5_2:11662568-11662587 Msa0757010:five_prime_UTR 30.0%
!!! AACCCTTACCTCTTTTTTAT+TGG - chr5_2:11662573-11662592 None:intergenic 30.0%
! AGAAGAGTGCAAAGATTTAA+GGG - chr5_2:11662623-11662642 None:intergenic 30.0%
! AAGAAGAGTGCAAAGATTTA+AGG - chr5_2:11662624-11662643 None:intergenic 30.0%
ATGATTATGCATGATGATTC+TGG + chr5_2:11663546-11663565 Msa0757010:three_prime_UTR 30.0%
GTTAAATTAATAACCACGGT+TGG - chr5_2:11663620-11663639 None:intergenic 30.0%
! ACACATGATTGTAACTTGTA+TGG - chr5_2:11663642-11663661 None:intergenic 30.0%
TTTAGGTACCAAAACAATCT+GGG - chr5_2:11663728-11663747 None:intergenic 30.0%
!! ATTGATATGTTACTTGCTGT+CGG + chr5_2:11663763-11663782 Msa0757010:three_prime_UTR 30.0%
! TATTGTCTAAAATGTGACTG+TGG + chr5_2:11663814-11663833 Msa0757010:three_prime_UTR 30.0%
! TTTTGTGTCAGAAAGAAAGA+CGG + chr5_2:11663903-11663922 Msa0757010:three_prime_UTR 30.0%
CTACACAATGAATACCAAAA+AGG - chr5_2:11663978-11663997 None:intergenic 30.0%
!! CTTTTTGGTATTCATTGTGT+AGG + chr5_2:11663976-11663995 Msa0757010:three_prime_UTR 30.0%
GGACCAATAAAAAAGAGGTA+AGG + chr5_2:11662567-11662586 Msa0757010:five_prime_UTR 35.0%
!!! TTGTTTTGTTTTGGATTCGG+CGG - chr5_2:11662661-11662680 None:intergenic 35.0%
GTAAAGGAAACTGGAAGAAT+CGG + chr5_2:11662908-11662927 Msa0757010:CDS 35.0%
CTCAAAGCTTGCATTGAATT+AGG + chr5_2:11662967-11662986 Msa0757010:CDS 35.0%
! GCTTGCATTGAATTAGGTTT+TGG + chr5_2:11662973-11662992 Msa0757010:CDS 35.0%
TGAAGATGATGATCCACAAA+CGG + chr5_2:11663464-11663483 Msa0757010:intron 35.0%
ATTTCACTACCAAACAGAAC+CGG + chr5_2:11663678-11663697 Msa0757010:three_prime_UTR 35.0%
! TCTGGGTTTGGTTAAACTAA+AGG - chr5_2:11663711-11663730 None:intergenic 35.0%
! TAACCAAACCCAGATTGTTT+TGG + chr5_2:11663717-11663736 Msa0757010:three_prime_UTR 35.0%
CTTTAGGTACCAAAACAATC+TGG - chr5_2:11663729-11663748 None:intergenic 35.0%
GGACCAATAAAAAAGAGGTA+AGG + chr5_2:11662567-11662586 Msa0757010:five_prime_UTR 35.0%
!!! TTGTTTTGTTTTGGATTCGG+CGG - chr5_2:11662661-11662680 None:intergenic 35.0%
GTAAAGGAAACTGGAAGAAT+CGG + chr5_2:11662908-11662927 Msa0757010:CDS 35.0%
CTCAAAGCTTGCATTGAATT+AGG + chr5_2:11662967-11662986 Msa0757010:CDS 35.0%
! GCTTGCATTGAATTAGGTTT+TGG + chr5_2:11662973-11662992 Msa0757010:CDS 35.0%
TGAAGATGATGATCCACAAA+CGG + chr5_2:11663464-11663483 Msa0757010:intron 35.0%
ATTTCACTACCAAACAGAAC+CGG + chr5_2:11663678-11663697 Msa0757010:three_prime_UTR 35.0%
! TCTGGGTTTGGTTAAACTAA+AGG - chr5_2:11663711-11663730 None:intergenic 35.0%
! TAACCAAACCCAGATTGTTT+TGG + chr5_2:11663717-11663736 Msa0757010:three_prime_UTR 35.0%
CTTTAGGTACCAAAACAATC+TGG - chr5_2:11663729-11663748 None:intergenic 35.0%
! AAAGATTTAAGGGTGTGGTG+AGG - chr5_2:11662613-11662632 None:intergenic 40.0%
AGTGCAAAGATTTAAGGGTG+TGG - chr5_2:11662618-11662637 None:intergenic 40.0%
!!! GATTCGGCGGTTTTTTGAAT+GGG - chr5_2:11662648-11662667 None:intergenic 40.0%
ACGACGATCAGTTTCAACTT+CGG - chr5_2:11663013-11663032 None:intergenic 40.0%
GCATGATAAAGTTCAAGAGC+AGG - chr5_2:11663051-11663070 None:intergenic 40.0%
AATATTACCGTTGCCGAAGA+TGG - chr5_2:11663193-11663212 None:intergenic 40.0%
ATGCATGATGATTCTGGTGA+TGG + chr5_2:11663552-11663571 Msa0757010:three_prime_UTR 40.0%
TTGGTTAAACTAAAGGCTCG+TGG - chr5_2:11663704-11663723 None:intergenic 40.0%
GTACCAAAACAATCTGGGTT+TGG - chr5_2:11663723-11663742 None:intergenic 40.0%
AATACCAAAAAGGAGCAGCA+GGG - chr5_2:11663968-11663987 None:intergenic 40.0%
TGGTATTCATTGTGTAGGTG+TGG + chr5_2:11663981-11664000 Msa0757010:three_prime_UTR 40.0%
! AAAGATTTAAGGGTGTGGTG+AGG - chr5_2:11662613-11662632 None:intergenic 40.0%
AGTGCAAAGATTTAAGGGTG+TGG - chr5_2:11662618-11662637 None:intergenic 40.0%
!!! GATTCGGCGGTTTTTTGAAT+GGG - chr5_2:11662648-11662667 None:intergenic 40.0%
ACGACGATCAGTTTCAACTT+CGG - chr5_2:11663013-11663032 None:intergenic 40.0%
GCATGATAAAGTTCAAGAGC+AGG - chr5_2:11663051-11663070 None:intergenic 40.0%
AATATTACCGTTGCCGAAGA+TGG - chr5_2:11663193-11663212 None:intergenic 40.0%
ATGCATGATGATTCTGGTGA+TGG + chr5_2:11663552-11663571 Msa0757010:three_prime_UTR 40.0%
TTGGTTAAACTAAAGGCTCG+TGG - chr5_2:11663704-11663723 None:intergenic 40.0%
GTACCAAAACAATCTGGGTT+TGG - chr5_2:11663723-11663742 None:intergenic 40.0%
AATACCAAAAAGGAGCAGCA+GGG - chr5_2:11663968-11663987 None:intergenic 40.0%
TGGTATTCATTGTGTAGGTG+TGG + chr5_2:11663981-11664000 Msa0757010:three_prime_UTR 40.0%
!!! GGATTCGGCGGTTTTTTGAA+TGG - chr5_2:11662649-11662668 None:intergenic 45.0%
GGAAGACGCTATGGCTTATT+AGG - chr5_2:11662698-11662717 None:intergenic 45.0%
AATTCTTGCCTTAGCAGTGG+TGG - chr5_2:11662820-11662839 None:intergenic 45.0%
GAGAATTCTTGCCTTAGCAG+TGG - chr5_2:11662823-11662842 None:intergenic 45.0%
CGACGATCAGTTTCAACTTC+GGG - chr5_2:11663012-11663031 None:intergenic 45.0%
ATCCACAAACGGTGAAGACA+AGG + chr5_2:11663475-11663494 Msa0757010:CDS 45.0%
AACCTTGTCTTCACCGTTTG+TGG - chr5_2:11663480-11663499 None:intergenic 45.0%
TGAAGACAAGGTTGAGACAG+TGG + chr5_2:11663487-11663506 Msa0757010:CDS 45.0%
GAAGACAAGGTTGAGACAGT+GGG + chr5_2:11663488-11663507 Msa0757010:CDS 45.0%
! ACCACGGTTGGATTTTTCTC+CGG - chr5_2:11663608-11663627 None:intergenic 45.0%
TTAAACTAAAGGCTCGTGGC+CGG - chr5_2:11663700-11663719 None:intergenic 45.0%
ATACCAAAAAGGAGCAGCAG+GGG - chr5_2:11663967-11663986 None:intergenic 45.0%
GAATACCAAAAAGGAGCAGC+AGG - chr5_2:11663969-11663988 None:intergenic 45.0%
!!! GGATTCGGCGGTTTTTTGAA+TGG - chr5_2:11662649-11662668 None:intergenic 45.0%
GGAAGACGCTATGGCTTATT+AGG - chr5_2:11662698-11662717 None:intergenic 45.0%
AATTCTTGCCTTAGCAGTGG+TGG - chr5_2:11662820-11662839 None:intergenic 45.0%
GAGAATTCTTGCCTTAGCAG+TGG - chr5_2:11662823-11662842 None:intergenic 45.0%
CGACGATCAGTTTCAACTTC+GGG - chr5_2:11663012-11663031 None:intergenic 45.0%
ATCCACAAACGGTGAAGACA+AGG + chr5_2:11663475-11663494 Msa0757010:CDS 45.0%
AACCTTGTCTTCACCGTTTG+TGG - chr5_2:11663480-11663499 None:intergenic 45.0%
TGAAGACAAGGTTGAGACAG+TGG + chr5_2:11663487-11663506 Msa0757010:CDS 45.0%
GAAGACAAGGTTGAGACAGT+GGG + chr5_2:11663488-11663507 Msa0757010:CDS 45.0%
! ACCACGGTTGGATTTTTCTC+CGG - chr5_2:11663608-11663627 None:intergenic 45.0%
TTAAACTAAAGGCTCGTGGC+CGG - chr5_2:11663700-11663719 None:intergenic 45.0%
ATACCAAAAAGGAGCAGCAG+GGG - chr5_2:11663967-11663986 None:intergenic 45.0%
GAATACCAAAAAGGAGCAGC+AGG - chr5_2:11663969-11663988 None:intergenic 45.0%
GGTGAGGTGGTAGATCAAGT+TGG - chr5_2:11662597-11662616 None:intergenic 50.0%
! GATTTAAGGGTGTGGTGAGG+TGG - chr5_2:11662610-11662629 None:intergenic 50.0%
!! AGAAAGGAAGGTGGTGGAGA+TGG - chr5_2:11662787-11662806 None:intergenic 50.0%
TGGAAGAGAAAGGAAGGTGG+TGG - chr5_2:11662793-11662812 None:intergenic 50.0%
GCTTCGTCACGGTAAAGATC+AGG - chr5_2:11662877-11662896 None:intergenic 50.0%
ATCTTTACCGTGACGAAGCG+TGG + chr5_2:11662878-11662897 Msa0757010:CDS 50.0%
GGCTTCGTCGTAAAGGAAAC+TGG + chr5_2:11662899-11662918 Msa0757010:CDS 50.0%
! TAGTAACGGCGGCGGATTTA+GGG - chr5_2:11663104-11663123 None:intergenic 50.0%
GGAGGAAGACGGTGTAGTAA+CGG - chr5_2:11663118-11663137 None:intergenic 50.0%
!! AGAAGGAGTGCCATCACGAT+CGG - chr5_2:11663148-11663167 None:intergenic 50.0%
ATTACCGTTGCCGAAGATGG+CGG - chr5_2:11663190-11663209 None:intergenic 50.0%
ACCGGAGAAAAATCCAACCG+TGG + chr5_2:11663604-11663623 Msa0757010:three_prime_UTR 50.0%
! AATCCCCTGCTGCTCCTTTT+TGG + chr5_2:11663961-11663980 Msa0757010:three_prime_UTR 50.0%
GGTGAGGTGGTAGATCAAGT+TGG - chr5_2:11662597-11662616 None:intergenic 50.0%
! GATTTAAGGGTGTGGTGAGG+TGG - chr5_2:11662610-11662629 None:intergenic 50.0%
!! AGAAAGGAAGGTGGTGGAGA+TGG - chr5_2:11662787-11662806 None:intergenic 50.0%
TGGAAGAGAAAGGAAGGTGG+TGG - chr5_2:11662793-11662812 None:intergenic 50.0%
GCTTCGTCACGGTAAAGATC+AGG - chr5_2:11662877-11662896 None:intergenic 50.0%
ATCTTTACCGTGACGAAGCG+TGG + chr5_2:11662878-11662897 Msa0757010:CDS 50.0%
GGCTTCGTCGTAAAGGAAAC+TGG + chr5_2:11662899-11662918 Msa0757010:CDS 50.0%
! TAGTAACGGCGGCGGATTTA+GGG - chr5_2:11663104-11663123 None:intergenic 50.0%
GGAGGAAGACGGTGTAGTAA+CGG - chr5_2:11663118-11663137 None:intergenic 50.0%
!! AGAAGGAGTGCCATCACGAT+CGG - chr5_2:11663148-11663167 None:intergenic 50.0%
ATTACCGTTGCCGAAGATGG+CGG - chr5_2:11663190-11663209 None:intergenic 50.0%
ACCGGAGAAAAATCCAACCG+TGG + chr5_2:11663604-11663623 Msa0757010:three_prime_UTR 50.0%
! AATCCCCTGCTGCTCCTTTT+TGG + chr5_2:11663961-11663980 Msa0757010:three_prime_UTR 50.0%
!! GACATCGGAGCGTTGTTGTG+CGG - chr5_2:11662748-11662767 None:intergenic 55.0%
!! AAGGAAGGTGGTGGAGATGG+CGG - chr5_2:11662784-11662803 None:intergenic 55.0%
CGGTGGAAGAGAAAGGAAGG+TGG - chr5_2:11662796-11662815 None:intergenic 55.0%
GGACGGTGGAAGAGAAAGGA+AGG - chr5_2:11662799-11662818 None:intergenic 55.0%
! TGGTGGACGGTGGAAGAGAA+AGG - chr5_2:11662803-11662822 None:intergenic 55.0%
! CTTGCCTTAGCAGTGGTGGA+CGG - chr5_2:11662816-11662835 None:intergenic 55.0%
GAAGCGTGGCTTCGTCGTAA+AGG + chr5_2:11662892-11662911 Msa0757010:CDS 55.0%
! GTAGTAACGGCGGCGGATTT+AGG - chr5_2:11663105-11663124 None:intergenic 55.0%
GGAAGACGGTGTAGTAACGG+CGG - chr5_2:11663115-11663134 None:intergenic 55.0%
AAGGTTGAGACAGTGGGCAC+AGG + chr5_2:11663494-11663513 Msa0757010:CDS 55.0%
! GGCACAGGTTGTTGCTTGTG+CGG + chr5_2:11663509-11663528 Msa0757010:CDS 55.0%
!! GACATCGGAGCGTTGTTGTG+CGG - chr5_2:11662748-11662767 None:intergenic 55.0%
!! AAGGAAGGTGGTGGAGATGG+CGG - chr5_2:11662784-11662803 None:intergenic 55.0%
CGGTGGAAGAGAAAGGAAGG+TGG - chr5_2:11662796-11662815 None:intergenic 55.0%
GGACGGTGGAAGAGAAAGGA+AGG - chr5_2:11662799-11662818 None:intergenic 55.0%
! TGGTGGACGGTGGAAGAGAA+AGG - chr5_2:11662803-11662822 None:intergenic 55.0%
! CTTGCCTTAGCAGTGGTGGA+CGG - chr5_2:11662816-11662835 None:intergenic 55.0%
GAAGCGTGGCTTCGTCGTAA+AGG + chr5_2:11662892-11662911 Msa0757010:CDS 55.0%
! GTAGTAACGGCGGCGGATTT+AGG - chr5_2:11663105-11663124 None:intergenic 55.0%
GGAAGACGGTGTAGTAACGG+CGG - chr5_2:11663115-11663134 None:intergenic 55.0%
AAGGTTGAGACAGTGGGCAC+AGG + chr5_2:11663494-11663513 Msa0757010:CDS 55.0%
! GGCACAGGTTGTTGCTTGTG+CGG + chr5_2:11663509-11663528 Msa0757010:CDS 55.0%
CGGTGCGTTGGAAGACGCTA+TGG - chr5_2:11662707-11662726 None:intergenic 60.0%
CGTTGTTGTGCGGAGGTCGA+GGG - chr5_2:11662738-11662757 None:intergenic 60.0%
!! ATCGGAGCGTTGTTGTGCGG+AGG - chr5_2:11662745-11662764 None:intergenic 60.0%
GGCGCGTAGGGATCAGACAT+CGG - chr5_2:11662763-11662782 None:intergenic 60.0%
TCCACCGTCCACCACTGCTA+AGG + chr5_2:11662809-11662828 Msa0757010:CDS 60.0%
CACGATCGCTGCTTGAGGAG+AGG - chr5_2:11662850-11662869 None:intergenic 60.0%
CTCTCCTCAAGCAGCGATCG+TGG + chr5_2:11662848-11662867 Msa0757010:CDS 60.0%
GACGAAGCCACGCTTCGTCA+CGG - chr5_2:11662888-11662907 None:intergenic 60.0%
AGACGGTGTAGTAACGGCGG+CGG - chr5_2:11663112-11663131 None:intergenic 60.0%
!! AGGAGTGCCATCACGATCGG+AGG - chr5_2:11663145-11663164 None:intergenic 60.0%
GATGGCACTCCTTCTCCTCG+TGG + chr5_2:11663153-11663172 Msa0757010:CDS 60.0%
CGGTGCGTTGGAAGACGCTA+TGG - chr5_2:11662707-11662726 None:intergenic 60.0%
CGTTGTTGTGCGGAGGTCGA+GGG - chr5_2:11662738-11662757 None:intergenic 60.0%
!! ATCGGAGCGTTGTTGTGCGG+AGG - chr5_2:11662745-11662764 None:intergenic 60.0%
GGCGCGTAGGGATCAGACAT+CGG - chr5_2:11662763-11662782 None:intergenic 60.0%
TCCACCGTCCACCACTGCTA+AGG + chr5_2:11662809-11662828 Msa0757010:CDS 60.0%
CACGATCGCTGCTTGAGGAG+AGG - chr5_2:11662850-11662869 None:intergenic 60.0%
CTCTCCTCAAGCAGCGATCG+TGG + chr5_2:11662848-11662867 Msa0757010:CDS 60.0%
GACGAAGCCACGCTTCGTCA+CGG - chr5_2:11662888-11662907 None:intergenic 60.0%
AGACGGTGTAGTAACGGCGG+CGG - chr5_2:11663112-11663131 None:intergenic 60.0%
!! AGGAGTGCCATCACGATCGG+AGG - chr5_2:11663145-11663164 None:intergenic 60.0%
GATGGCACTCCTTCTCCTCG+TGG + chr5_2:11663153-11663172 Msa0757010:CDS 60.0%
GTCTTCCAACGCACCGTGCG+AGG + chr5_2:11662711-11662730 Msa0757010:five_prime_UTR 65.0%
GCGTTGTTGTGCGGAGGTCG+AGG - chr5_2:11662739-11662758 None:intergenic 65.0%
!! GCCTTAGCAGTGGTGGACGG+TGG - chr5_2:11662813-11662832 None:intergenic 65.0%
GCGACCACGATCGCTGCTTG+AGG - chr5_2:11662855-11662874 None:intergenic 65.0%
TCGGAGGCTGCGGAGGAAGA+CGG - chr5_2:11663129-11663148 None:intergenic 65.0%
ATCACGATCGGAGGCTGCGG+AGG - chr5_2:11663136-11663155 None:intergenic 65.0%
TCCGCAGCCTCCGATCGTGA+TGG + chr5_2:11663135-11663154 Msa0757010:CDS 65.0%
GCCATCACGATCGGAGGCTG+CGG - chr5_2:11663139-11663158 None:intergenic 65.0%
AGCCCTCGCACCGCCATCTT+CGG + chr5_2:11663177-11663196 Msa0757010:CDS 65.0%
! TGCCGAAGATGGCGGTGCGA+GGG - chr5_2:11663182-11663201 None:intergenic 65.0%
! TTGCCGAAGATGGCGGTGCG+AGG - chr5_2:11663183-11663202 None:intergenic 65.0%
CGCACCGCCATCTTCGGCAA+CGG + chr5_2:11663183-11663202 Msa0757010:CDS 65.0%
! GGCTCGTGGCCGGTTCTGTT+TGG - chr5_2:11663690-11663709 None:intergenic 65.0%
GTCTTCCAACGCACCGTGCG+AGG + chr5_2:11662711-11662730 Msa0757010:five_prime_UTR 65.0%
GCGTTGTTGTGCGGAGGTCG+AGG - chr5_2:11662739-11662758 None:intergenic 65.0%
!! GCCTTAGCAGTGGTGGACGG+TGG - chr5_2:11662813-11662832 None:intergenic 65.0%
GCGACCACGATCGCTGCTTG+AGG - chr5_2:11662855-11662874 None:intergenic 65.0%
TCGGAGGCTGCGGAGGAAGA+CGG - chr5_2:11663129-11663148 None:intergenic 65.0%
ATCACGATCGGAGGCTGCGG+AGG - chr5_2:11663136-11663155 None:intergenic 65.0%
TCCGCAGCCTCCGATCGTGA+TGG + chr5_2:11663135-11663154 Msa0757010:CDS 65.0%
GCCATCACGATCGGAGGCTG+CGG - chr5_2:11663139-11663158 None:intergenic 65.0%
AGCCCTCGCACCGCCATCTT+CGG + chr5_2:11663177-11663196 Msa0757010:CDS 65.0%
! TGCCGAAGATGGCGGTGCGA+GGG - chr5_2:11663182-11663201 None:intergenic 65.0%
! TTGCCGAAGATGGCGGTGCG+AGG - chr5_2:11663183-11663202 None:intergenic 65.0%
CGCACCGCCATCTTCGGCAA+CGG + chr5_2:11663183-11663202 Msa0757010:CDS 65.0%
! GGCTCGTGGCCGGTTCTGTT+TGG - chr5_2:11663690-11663709 None:intergenic 65.0%
AGGGACCTCGCACGGTGCGT+TGG - chr5_2:11662719-11662738 None:intergenic 70.0%
GGAGGTCGAGGGACCTCGCA+CGG - chr5_2:11662727-11662746 None:intergenic 70.0%
! GGTGGAGATGGCGGCGCGTA+GGG - chr5_2:11662775-11662794 None:intergenic 70.0%
! TGGTGGAGATGGCGGCGCGT+AGG - chr5_2:11662776-11662795 None:intergenic 70.0%
CGAGGGCTGCCACGAGGAGA+AGG - chr5_2:11663165-11663184 None:intergenic 70.0%
AGGGACCTCGCACGGTGCGT+TGG - chr5_2:11662719-11662738 None:intergenic 70.0%
GGAGGTCGAGGGACCTCGCA+CGG - chr5_2:11662727-11662746 None:intergenic 70.0%
! GGTGGAGATGGCGGCGCGTA+GGG - chr5_2:11662775-11662794 None:intergenic 70.0%
! TGGTGGAGATGGCGGCGCGT+AGG - chr5_2:11662776-11662795 None:intergenic 70.0%
CGAGGGCTGCCACGAGGAGA+AGG - chr5_2:11663165-11663184 None:intergenic 70.0%
! GCGGTGCGAGGGCTGCCACG+AGG - chr5_2:11663171-11663190 None:intergenic 80.0%
! GCGGTGCGAGGGCTGCCACG+AGG - chr5_2:11663171-11663190 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 11662565 11664054 11662565 ID=Msa0757010;Name=Msa0757010
chr5_2 mRNA 11662565 11664054 11662565 ID=Msa0757010-mRNA-1;Parent=Msa0757010;Name=Msa0757010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=198|1|1|1|1|1|2|509|172
chr5_2 exon 11662565 11663204 11662565 ID=Msa0757010-mRNA-1:exon:5249;Parent=Msa0757010-mRNA-1
chr5_2 exon 11663469 11664054 11663469 ID=Msa0757010-mRNA-1:exon:5250;Parent=Msa0757010-mRNA-1
chr5_2 five_prime_UTR 11662565 11662762 11662565 ID=Msa0757010-mRNA-1:five_prime_utr;Parent=Msa0757010-mRNA-1
chr5_2 CDS 11662763 11663204 11662763 ID=Msa0757010-mRNA-1:cds;Parent=Msa0757010-mRNA-1
chr5_2 CDS 11663469 11663545 11663469 ID=Msa0757010-mRNA-1:cds;Parent=Msa0757010-mRNA-1
chr5_2 three_prime_UTR 11663546 11664054 11663546 ID=Msa0757010-mRNA-1:three_prime_utr;Parent=Msa0757010-mRNA-1
Gene Sequence

>Msa0757010

ATGTCTGATCCCTACGCGCCGCCATCTCCACCACCTTCCTTTCTCTTCCACCGTCCACCACTGCTAAGGCAAGAATTCTCTTCTCCTCTCCTCAAGCAGCGATCGTGGTCGCCTGATCTTTACCGTGACGAAGCGTGGCTTCGTCGTAAAGGAAACTGGAAGAATCGGAGAAGTAAAAGCGTTACTGATGAAGATGTTGATGAACTCAAAGCTTGCATTGAATTAGGTTTTGGATTTGAATCTTCACCCGAAGTTGAAACTGATCGTCGTCTTGTTGATACTTTACCTGCTCTTGAACTTTATCATGCTGTTAATAAAAATTATAATGATTCTCGGAACCCTAAATCCGCCGCCGTTACTACACCGTCTTCCTCCGCAGCCTCCGATCGTGATGGCACTCCTTCTCCTCGTGGCAGCCCTCGCACCGCCATCTTCGGCAACGATGATGATCCACAAACGGTGAAGACAAGGTTGAGACAGTGGGCACAGGTTGTTGCTTGTGCGGTTCGTCAAAGCTGA
Protein sequence

>Msa0757010

MSDPYAPPSPPPSFLFHRPPLLRQEFSSPLLKQRSWSPDLYRDEAWLRRKGNWKNRRSKSVTDEDVDELKACIELGFGFESSPEVETDRRLVDTLPALELYHAVNKNYNDSRNPKSAAVTTPSSSAASDRDGTPSPRGSPRTAIFGNDDDPQTVKTRLRQWAQVVACAVRQS*