AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0561540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0561540 MtrunA17_Chr4g0052111 96.565 262 9 0 44 305 1 262 0.0 530
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0561540 AT1G29950.2 41.126 231 115 7 92 304 18 245 3.31e-38 135
Msa0561540 AT1G29950.1 41.126 231 115 7 92 304 18 245 3.31e-38 135

Find 101 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAAGACAATGAAATTCTT+AGG 0.183604 4_1:+61109491 None:intergenic
ACACTTTCAGGTCAGAGTTT+TGG 0.217965 4_1:-61111107 Msa0561540:intron
AATGGACACCGTTACCGTTT+TGG 0.230492 4_1:-61109036 Msa0561540:CDS
GTTTGTCATTAAAGATATTC+TGG 0.235805 4_1:-61111084 Msa0561540:five_prime_UTR
CCTTGATTTCGTGCAGTAAC+TGG 0.271176 4_1:+61109193 None:intergenic
GATTAGATCGACATTGTTCT+TGG 0.294094 4_1:+61111835 None:intergenic
GCTCCACATGGTTCTGCCTT+TGG 0.304746 4_1:-61109623 Msa0561540:CDS
GCTCCACATGCTTCTGCTTT+CGG 0.312643 4_1:-61109659 Msa0561540:CDS
AAACGCAAACAATGCTGATT+TGG 0.336184 4_1:+61109787 None:intergenic
TTAAACTTGTAGGTCGTTGC+AGG 0.347647 4_1:+61109434 None:intergenic
TCTTGCTGCTGCAGGTGATT+AGG 0.358521 4_1:-61110668 Msa0561540:intron
AAAGGACGTACTGCCTTTCC+CGG 0.382439 4_1:+61109713 None:intergenic
AAACACAATTACTCTTGAAA+AGG 0.409317 4_1:+61111030 None:intergenic
TCAATTGATGTTGGTTTGCC+AGG 0.415900 4_1:-61110531 Msa0561540:intron
GATTTGTGAGGAAGAGGAAA+AGG 0.423075 4_1:+61111647 None:intergenic
ATCAAACAGATCAACAAAAC+AGG 0.423904 4_1:-61109469 Msa0561540:CDS
TGAAGATGGATCAGAGAAGA+AGG 0.425988 4_1:+61111684 None:intergenic
GAAGGATAAAATTAATCAAA+TGG 0.430828 4_1:-61109366 Msa0561540:CDS
AAACGGTAACGGTGTCCATT+TGG 0.432703 4_1:+61109039 None:intergenic
ACTATCATACCCGCGATAAA+CGG 0.433083 4_1:-61111868 Msa0561540:five_prime_UTR
TCCATTTGGTTGCCTCCACC+AGG 0.433945 4_1:+61109053 None:intergenic
AGAATGTTCATTGCCAAAGT+TGG 0.444081 4_1:-61110590 Msa0561540:CDS
CAAGAGTAATTGTGTTTGCT+TGG 0.450947 4_1:-61111024 Msa0561540:five_prime_UTR
GGGAAAGGCAGTACGTCCTT+TGG 0.456169 4_1:-61109711 Msa0561540:CDS
TCATCCGCACATAACTCGTC+AGG 0.457509 4_1:-61109155 Msa0561540:CDS
GAATGTTCATTGCCAAAGTT+GGG 0.458415 4_1:-61110589 Msa0561540:CDS
CAAAGACAATGAAATTCTTA+GGG 0.464787 4_1:+61109492 None:intergenic
TCCTCTTCCTCACAAATCCT+AGG 0.466152 4_1:-61111642 Msa0561540:intron
AGAAATATCGTGCCTGGTGG+AGG 0.468154 4_1:-61109065 Msa0561540:CDS
TGCAAGTTAAACCTTTCAAA+TGG 0.471021 4_1:+61109530 None:intergenic
TGAGATCAATCAAGATGCCT+TGG 0.471766 4_1:-61111058 Msa0561540:five_prime_UTR
TGCATACAGTCAATTGATGT+TGG 0.473587 4_1:-61110540 Msa0561540:CDS
ACCTAGGATTTGTGAGGAAG+AGG 0.475710 4_1:+61111641 None:intergenic
CCAGTTACTGCACGAAATCA+AGG 0.478607 4_1:-61109193 Msa0561540:CDS
GCCGGGACTGTTAAACTTGT+AGG 0.478819 4_1:+61109424 None:intergenic
GAGTAATTGTGTTTGCTTGG+TGG 0.478991 4_1:-61111021 Msa0561540:five_prime_UTR
GAAGAGGAAAAGGGTGTGAA+AGG 0.482672 4_1:+61111657 None:intergenic
TAAAATTAATCAAATGGAAA+CGG 0.485866 4_1:-61109360 Msa0561540:CDS
ACCTACAAGTTTAACAGTCC+CGG 0.485914 4_1:-61109425 Msa0561540:CDS
AACACAATTACTCTTGAAAA+GGG 0.487308 4_1:+61111031 None:intergenic
AAGGTTGAAGTCGAGCAATT+CGG 0.492781 4_1:-61108984 Msa0561540:CDS
GCCTGGTGGAGGCAACCAAA+TGG 0.494134 4_1:-61109054 Msa0561540:CDS
ATTTGTGAGGAAGAGGAAAA+GGG 0.496480 4_1:+61111648 None:intergenic
CTTCTAGTTCATCCGAGTCT+AGG 0.499152 4_1:+61109285 None:intergenic
ACTCGCACTTGGAGGTAAAA+CGG 0.500512 4_1:+61109562 None:intergenic
TGCGTTTAGTTTAGTGAGAG+TGG 0.504635 4_1:-61109771 Msa0561540:CDS
GTCAGATAACACCATTTGAA+AGG 0.508642 4_1:-61109541 Msa0561540:CDS
GATATGAAGGCGTGATGGGA+TGG 0.510194 4_1:+61111785 None:intergenic
TTCAAGATATGAAGGCGTGA+TGG 0.518579 4_1:+61111780 None:intergenic
GAAGATGGATCAGAGAAGAA+GGG 0.521999 4_1:+61111685 None:intergenic
GGTGTTATCTGACTCGCACT+TGG 0.524930 4_1:+61109551 None:intergenic
CAGTGAAAAGAAACAACACT+TGG 0.526852 4_1:-61109114 Msa0561540:CDS
TCAAGATATGAAGGCGTGAT+GGG 0.526920 4_1:+61111781 None:intergenic
AGTAGCATGAGTGTTGAAGC+CGG 0.530258 4_1:+61109406 None:intergenic
CATAACTCGTCAGGGACAAA+GGG 0.532535 4_1:-61109146 Msa0561540:CDS
GAAGTCGAGCAATTCGGAGT+TGG 0.534683 4_1:-61108978 Msa0561540:CDS
CGATTAGAGCACGCGTGAAG+AGG 0.535117 4_1:+61111898 None:intergenic
TGGAGGTAAAACGGCATCAA+AGG 0.535639 4_1:+61109571 None:intergenic
ACATAACTCGTCAGGGACAA+AGG 0.541406 4_1:-61109147 Msa0561540:CDS
AACAACACTTGGAGATGAAG+AGG 0.542117 4_1:-61109103 Msa0561540:CDS
GTGTGAAAGGAAATTGAAGA+TGG 0.542423 4_1:+61111670 None:intergenic
GTTATCTGACTCGCACTTGG+AGG 0.545030 4_1:+61109554 None:intergenic
ATATGAAGGCGTGATGGGAT+GGG 0.555384 4_1:+61111786 None:intergenic
TTCTTAGGGCAAACCTCAGA+GGG 0.559386 4_1:+61109506 None:intergenic
AATGCACTTCCGTTTATCGC+GGG 0.563337 4_1:+61111859 None:intergenic
TTCTGTAATTTAATTAGCTG+AGG 0.564309 4_1:-61110983 Msa0561540:intron
ACATGGATGATCTCAGCCGT+TGG 0.568907 4_1:+61111813 None:intergenic
AGCTTCGTCCAAAACGGTAA+CGG 0.571988 4_1:+61109028 None:intergenic
ATATTGAGAAATATCGTGCC+TGG 0.572011 4_1:-61109071 Msa0561540:CDS
AAAATACCTCAAGTCTCTCA+AGG 0.572321 4_1:-61109003 Msa0561540:CDS
GGGTACATTAAAATGAGTGT+TGG 0.573672 4_1:-61110569 Msa0561540:CDS
GTAGCATGAGTGTTGAAGCC+GGG 0.574493 4_1:+61109407 None:intergenic
TTTAGTTTAGTGAGAGTGGA+CGG 0.582673 4_1:-61109767 Msa0561540:CDS
TGCATTGTTGAGCCTAGACT+CGG 0.583179 4_1:-61109297 Msa0561540:CDS
CCTTTGGCAGATGAAGCCAT+TGG 0.588109 4_1:-61109695 Msa0561540:CDS
TGCAGGTGATTAGGCTGCAG+AGG 0.595624 4_1:-61110659 Msa0561540:intron
AAGGACGTACTGCCTTTCCC+GGG 0.597633 4_1:+61109714 None:intergenic
AGCATTCATATAAGTACCAA+AGG 0.603461 4_1:+61109607 None:intergenic
AGCATTCATATAAGTGCCAA+TGG 0.605224 4_1:+61109679 None:intergenic
ACTTGTAGGTCGTTGCAGGA+TGG 0.612223 4_1:+61109438 None:intergenic
GTACCGAAAGCAGAAGCATG+TGG 0.618840 4_1:+61109656 None:intergenic
GCACGAAATCAAGGAAGAAG+AGG 0.621640 4_1:-61109184 Msa0561540:CDS
CTTCAACCTTGAGAGACTTG+AGG 0.621750 4_1:+61108997 None:intergenic
GTTTAACTTGCAGCCCTCTG+AGG 0.624822 4_1:-61109519 Msa0561540:CDS
GTAACTGGAGCAAGTATCAG+AGG 0.624869 4_1:+61109208 None:intergenic
GTGATGGGATGGGTCAAACA+TGG 0.626921 4_1:+61111796 None:intergenic
GCATCAAAGGCAGAAGCATG+TGG 0.627838 4_1:+61109584 None:intergenic
ATTCTTAGGGCAAACCTCAG+AGG 0.643235 4_1:+61109505 None:intergenic
AAGAAGAAGTCAGCACTGCA+AGG 0.644914 4_1:-61109253 Msa0561540:CDS
CCAATGGCTTCATCTGCCAA+AGG 0.645058 4_1:+61109695 None:intergenic
AGCAAGACCTAGGATTTGTG+AGG 0.650279 4_1:+61111635 None:intergenic
TCACGCGTGCTCTAATCGAA+CGG 0.650884 4_1:-61111893 Msa0561540:five_prime_UTR
TTGAGAAATATCGTGCCTGG+TGG 0.655055 4_1:-61109068 Msa0561540:CDS
ACACTTGGAGATGAAGAGGA+TGG 0.657366 4_1:-61109099 Msa0561540:CDS
AAATGCACTTCCGTTTATCG+CGG 0.658821 4_1:+61111858 None:intergenic
CATCCGCACATAACTCGTCA+GGG 0.664019 4_1:-61109154 Msa0561540:CDS
ACTTACATGAATGCTCCACA+TGG 0.683680 4_1:-61109635 Msa0561540:CDS
ACAATGTCGATCTAATCCAA+CGG 0.685385 4_1:-61111829 Msa0561540:five_prime_UTR
TGTCCCTGACGAGTTATGTG+CGG 0.691893 4_1:+61109151 None:intergenic
GTACCAAAGGCAGAACCATG+TGG 0.694870 4_1:+61109620 None:intergenic
CTCTTGAAAAGGGAAAACCA+AGG 0.760665 4_1:+61111041 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGAAATTCTTTTTTTTTT+TGG - chr4_1:61109276-61109295 Msa0561540:CDS 10.0%
!!! TTGAAATTCTTTTTTTTTTT+GGG - chr4_1:61109277-61109296 Msa0561540:CDS 10.0%
!!! TAAAAATTTATTCAAGTTAA+TGG - chr4_1:61109493-61109512 Msa0561540:CDS 10.0%
!!! AAAAATTTATTCAAGTTAAT+GGG - chr4_1:61109494-61109513 Msa0561540:CDS 10.0%
!! AGAATTTCAAAAATCAAAAT+TGG + chr4_1:61109267-61109286 None:intergenic 15.0%
!!! TGTTAAAAAGTTTCTATTTT+TGG - chr4_1:61109322-61109341 Msa0561540:CDS 15.0%
!!! ATTTAGGATTTTTATATGTT+AGG - chr4_1:61109555-61109574 Msa0561540:CDS 15.0%
!!! TTCACTTTATTTATTTGTTT+TGG - chr4_1:61109634-61109653 Msa0561540:CDS 15.0%
!!! TTATTTATTTGTTTTGGATT+TGG - chr4_1:61109640-61109659 Msa0561540:CDS 15.0%
!! TAAAATTAATCAAATGGAAA+CGG - chr4_1:61111509-61111528 Msa0561540:intron 15.0%
!!! TATAGTTTTGATGAAAAGTT+TGG - chr4_1:61109448-61109467 Msa0561540:CDS 20.0%
!!! ATAGTTGATTTTGCATTTAA+TGG - chr4_1:61109726-61109745 Msa0561540:CDS 20.0%
!! CAAAATAAAGTATGAAACTA+AGG + chr4_1:61110097-61110116 None:intergenic 20.0%
!! ACATGAAATAGTAAAACATT+AGG + chr4_1:61110141-61110160 None:intergenic 20.0%
!! AAGTAAAAAACACTAATTAG+AGG + chr4_1:61110240-61110259 None:intergenic 20.0%
!! TACGAGATTTGAAAATTAAA+TGG - chr4_1:61110401-61110420 Msa0561540:intron 20.0%
!! GACATAAAAATATTCTCAAA+AGG - chr4_1:61110490-61110509 Msa0561540:intron 20.0%
!! ATAAAAATATTCTCAAAAGG+AGG - chr4_1:61110493-61110512 Msa0561540:intron 20.0%
!! TAATGAAGAATTTCAATACA+AGG + chr4_1:61110909-61110928 None:intergenic 20.0%
!! TATTGAAATTCTTCATTAGA+AGG - chr4_1:61110911-61110930 Msa0561540:intron 20.0%
!! AATTCCATCTAAACAAATAA+TGG + chr4_1:61110972-61110991 None:intergenic 20.0%
!! ATTATTTGTTTAGATGGAAT+TGG - chr4_1:61110971-61110990 Msa0561540:intron 20.0%
!! GAAGGATAAAATTAATCAAA+TGG - chr4_1:61111503-61111522 Msa0561540:intron 20.0%
!! CTAAGAAGATGAAATTTTTG+CGG + chr4_1:61109141-61109160 None:intergenic 25.0%
!! TTCGAATTGATTCATTGATT+TGG - chr4_1:61109418-61109437 Msa0561540:CDS 25.0%
!! ATGCTTTGTTAAATTCTAAC+TGG - chr4_1:61109585-61109604 Msa0561540:CDS 25.0%
!!! TTATGATTTTAGAGCTAGAT+TGG - chr4_1:61109663-61109682 Msa0561540:CDS 25.0%
! GTTTGTCATTAAAGATATTC+TGG - chr4_1:61109785-61109804 Msa0561540:CDS 25.0%
! AACACAATTACTCTTGAAAA+GGG + chr4_1:61109841-61109860 None:intergenic 25.0%
! AAACACAATTACTCTTGAAA+AGG + chr4_1:61109842-61109861 None:intergenic 25.0%
! TTCTGTAATTTAATTAGCTG+AGG - chr4_1:61109886-61109905 Msa0561540:intron 25.0%
!! TGAAGTCAGTATTATTGTTT+TGG - chr4_1:61109999-61110018 Msa0561540:intron 25.0%
!!! TCAGTATTATTGTTTTGGAT+TGG - chr4_1:61110004-61110023 Msa0561540:intron 25.0%
!!! TATTATTGTTTTGGATTGGT+TGG - chr4_1:61110008-61110027 Msa0561540:intron 25.0%
!!! ATTATTGTTTTGGATTGGTT+GGG - chr4_1:61110009-61110028 Msa0561540:intron 25.0%
! TAATATCTAGCATGCAAAAT+CGG + chr4_1:61110444-61110463 None:intergenic 25.0%
! CTAAAAATCTTCTCAAAAAG+TGG - chr4_1:61110536-61110555 Msa0561540:CDS 25.0%
! CAAAGACAATGAAATTCTTA+GGG + chr4_1:61111380-61111399 None:intergenic 25.0%
! TCAAAGACAATGAAATTCTT+AGG + chr4_1:61111381-61111400 None:intergenic 25.0%
CTGTTCTTGAGATTAGATTT+AGG - chr4_1:61109539-61109558 Msa0561540:CDS 30.0%
! AGGTAGTCTTTTACTTCTAT+TGG - chr4_1:61109690-61109709 Msa0561540:CDS 30.0%
!! ATCCTAATTTTGACACTTTC+AGG - chr4_1:61109750-61109769 Msa0561540:CDS 30.0%
ATTCTATAAACTATAGCAGC+AGG + chr4_1:61109933-61109952 None:intergenic 30.0%
TTCATAACTTCAACTTTCCA+CGG + chr4_1:61109983-61110002 None:intergenic 30.0%
!! CAGTGTATTGTTTCTATCTA+CGG + chr4_1:61110064-61110083 None:intergenic 30.0%
!! CATTTTAATGTACCCAACTT+TGG + chr4_1:61110295-61110314 None:intergenic 30.0%
! TAAATATTTCTCCCCTTTTC+TGG - chr4_1:61110361-61110380 Msa0561540:intron 30.0%
AAATCTCGTATCCAAAATCA+TGG + chr4_1:61110391-61110410 None:intergenic 30.0%
TGAGCTTCATTATCTTGTTA+TGG - chr4_1:61110596-61110615 Msa0561540:exon 30.0%
! CATTATCTTGTTATGGCTAA+TGG - chr4_1:61110603-61110622 Msa0561540:exon 30.0%
TATAAAGCAACATGCTTTGA+AGG - chr4_1:61110629-61110648 Msa0561540:five_prime_UTR 30.0%
TAAGGTAGCAATAATAGAAC+AGG + chr4_1:61110780-61110799 None:intergenic 30.0%
AGAAAACGAAGTGGTATATA+AGG + chr4_1:61110798-61110817 None:intergenic 30.0%
GTTGCCATTATTTGTTTAGA+TGG - chr4_1:61110965-61110984 Msa0561540:intron 30.0%
AAATTATTCTGTACTAGAGC+TGG + chr4_1:61111049-61111068 None:intergenic 30.0%
AGCATTCATATAAGTACCAA+AGG + chr4_1:61111265-61111284 None:intergenic 30.0%
TGCAAGTTAAACCTTTCAAA+TGG + chr4_1:61111342-61111361 None:intergenic 30.0%
ATCAAACAGATCAACAAAAC+AGG - chr4_1:61111400-61111419 Msa0561540:intron 30.0%
! ATTGTCATCTTCTTTTGTAG+AGG - chr4_1:61111533-61111552 Msa0561540:intron 30.0%
GATTAGATCGACATTGTTCT+TGG + chr4_1:61109037-61109056 None:intergenic 35.0%
ACAATGTCGATCTAATCCAA+CGG - chr4_1:61109040-61109059 Msa0561540:CDS 35.0%
!! GGAGCATTTTCAAGATATGA+AGG + chr4_1:61109100-61109119 None:intergenic 35.0%
GTGTGAAAGGAAATTGAAGA+TGG + chr4_1:61109202-61109221 None:intergenic 35.0%
ATTTGTGAGGAAGAGGAAAA+GGG + chr4_1:61109224-61109243 None:intergenic 35.0%
!! GACCTGAAAGTGTCAAAATT+AGG + chr4_1:61109755-61109774 None:intergenic 35.0%
CAAGAGTAATTGTGTTTGCT+TGG - chr4_1:61109845-61109864 Msa0561540:intron 35.0%
CTGATGTCGTATAAGCTATA+TGG + chr4_1:61110039-61110058 None:intergenic 35.0%
AGAATGTTCATTGCCAAAGT+TGG - chr4_1:61110279-61110298 Msa0561540:intron 35.0%
GAATGTTCATTGCCAAAGTT+GGG - chr4_1:61110280-61110299 Msa0561540:intron 35.0%
GGGTACATTAAAATGAGTGT+TGG - chr4_1:61110300-61110319 Msa0561540:intron 35.0%
TGCATACAGTCAATTGATGT+TGG - chr4_1:61110329-61110348 Msa0561540:intron 35.0%
AAAGGGGAGAAATATTTACC+TGG + chr4_1:61110359-61110378 None:intergenic 35.0%
AATCATGGCAATCCAGAAAA+GGG + chr4_1:61110376-61110395 None:intergenic 35.0%
AAATCATGGCAATCCAGAAA+AGG + chr4_1:61110377-61110396 None:intergenic 35.0%
! TTTCTGGATTGCCATGATTT+TGG - chr4_1:61110377-61110396 Msa0561540:intron 35.0%
!! CAACATGCTTTGAAGGTTAT+CGG - chr4_1:61110636-61110655 Msa0561540:five_prime_UTR 35.0%
GAACATACACACTTTGTGAA+TGG - chr4_1:61110668-61110687 Msa0561540:intron 35.0%
TTTAGTTGTTACATCCCTGT+AGG - chr4_1:61111014-61111033 Msa0561540:five_prime_UTR 35.0%
AGAATAATTTCCAGTTGCAC+AGG - chr4_1:61111059-61111078 Msa0561540:five_prime_UTR 35.0%
! AAACGCAAACAATGCTGATT+TGG + chr4_1:61111085-61111104 None:intergenic 35.0%
! TTTAGTTTAGTGAGAGTGGA+CGG - chr4_1:61111102-61111121 Msa0561540:intron 35.0%
AGCATTCATATAAGTGCCAA+TGG + chr4_1:61111193-61111212 None:intergenic 35.0%
GTCAGATAACACCATTTGAA+AGG - chr4_1:61111328-61111347 Msa0561540:intron 35.0%
!! TTGCACTCAAGATTTTGAGA+AGG - chr4_1:61111485-61111504 Msa0561540:intron 35.0%
! TTCTTTTGTAGAGGACTCTA+AGG - chr4_1:61111542-61111561 Msa0561540:intron 35.0%
CAGTGAAAAGAAACAACACT+TGG - chr4_1:61111755-61111774 Msa0561540:five_prime_UTR 35.0%
ATATTGAGAAATATCGTGCC+TGG - chr4_1:61111798-61111817 Msa0561540:five_prime_UTR 35.0%
! TTTTACAGCTTCGTCCAAAA+CGG + chr4_1:61111850-61111869 None:intergenic 35.0%
AAAATACCTCAAGTCTCTCA+AGG - chr4_1:61111866-61111885 Msa0561540:five_prime_UTR 35.0%
ACTATCATACCCGCGATAAA+CGG - chr4_1:61109001-61109020 Msa0561540:CDS 40.0%
AAATGCACTTCCGTTTATCG+CGG + chr4_1:61109014-61109033 None:intergenic 40.0%
! TCAAGATATGAAGGCGTGAT+GGG + chr4_1:61109091-61109110 None:intergenic 40.0%
! TTCAAGATATGAAGGCGTGA+TGG + chr4_1:61109092-61109111 None:intergenic 40.0%
!!! CGGAGAAGAGTTTTGAGATT+GGG + chr4_1:61109121-61109140 None:intergenic 40.0%
GAAGATGGATCAGAGAAGAA+GGG + chr4_1:61109187-61109206 None:intergenic 40.0%
TGAAGATGGATCAGAGAAGA+AGG + chr4_1:61109188-61109207 None:intergenic 40.0%
GATTTGTGAGGAAGAGGAAA+AGG + chr4_1:61109225-61109244 None:intergenic 40.0%
! TTTAGAGCTAGATTGGAGTG+AGG - chr4_1:61109670-61109689 Msa0561540:CDS 40.0%
!! ACACTTTCAGGTCAGAGTTT+TGG - chr4_1:61109762-61109781 Msa0561540:CDS 40.0%
!! CACTTTCAGGTCAGAGTTTT+GGG - chr4_1:61109763-61109782 Msa0561540:CDS 40.0%
TGAGATCAATCAAGATGCCT+TGG - chr4_1:61109811-61109830 Msa0561540:intron 40.0%
CTCTTGAAAAGGGAAAACCA+AGG + chr4_1:61109831-61109850 None:intergenic 40.0%
GAGTAATTGTGTTTGCTTGG+TGG - chr4_1:61109848-61109867 Msa0561540:intron 40.0%
!! TCAATTGATGTTGGTTTGCC+AGG - chr4_1:61110338-61110357 Msa0561540:intron 40.0%
ATCATGGCAATCCAGAAAAG+GGG + chr4_1:61110375-61110394 None:intergenic 40.0%
TATCTAGCATGCAAAATCGG+AGG + chr4_1:61110441-61110460 None:intergenic 40.0%
TACACACTTTGTGAATGGCA+TGG - chr4_1:61110673-61110692 Msa0561540:intron 40.0%
! ACCACTTCGTTTTCTTATGC+AGG - chr4_1:61110803-61110822 Msa0561540:intron 40.0%
TTGAAGCTAGACTACGTAAC+AGG + chr4_1:61110855-61110874 None:intergenic 40.0%
! TGTTACGTAGTCTAGCTTCA+AGG - chr4_1:61110854-61110873 Msa0561540:intron 40.0%
! AGCTTCAAGGAACGTTGATT+TGG - chr4_1:61110867-61110886 Msa0561540:intron 40.0%
!! GCTTCAAGGAACGTTGATTT+GGG - chr4_1:61110868-61110887 Msa0561540:intron 40.0%
!! AAGGAACGTTGATTTGGGTT+TGG - chr4_1:61110873-61110892 Msa0561540:intron 40.0%
!! AGGAACGTTGATTTGGGTTT+GGG - chr4_1:61110874-61110893 Msa0561540:intron 40.0%
GGATGTAACAACTAAACAGC+AGG + chr4_1:61111010-61111029 None:intergenic 40.0%
TATTCTGTACTAGAGCTGGA+TGG + chr4_1:61111045-61111064 None:intergenic 40.0%
! TGCGTTTAGTTTAGTGAGAG+TGG - chr4_1:61111098-61111117 Msa0561540:five_prime_UTR 40.0%
ACTTACATGAATGCTCCACA+TGG - chr4_1:61111234-61111253 Msa0561540:intron 40.0%
TTAAACTTGTAGGTCGTTGC+AGG + chr4_1:61111438-61111457 None:intergenic 40.0%
ACCTACAAGTTTAACAGTCC+CGG - chr4_1:61111444-61111463 Msa0561540:intron 40.0%
AACAACACTTGGAGATGAAG+AGG - chr4_1:61111766-61111785 Msa0561540:five_prime_UTR 40.0%
AAGGTTGAAGTCGAGCAATT+CGG - chr4_1:61111885-61111904 Msa0561540:five_prime_UTR 40.0%
AATGCACTTCCGTTTATCGC+GGG + chr4_1:61109013-61109032 None:intergenic 45.0%
! ATATGAAGGCGTGATGGGAT+GGG + chr4_1:61109086-61109105 None:intergenic 45.0%
!!! GCGGAGAAGAGTTTTGAGAT+TGG + chr4_1:61109122-61109141 None:intergenic 45.0%
! GAAGAGGAAAAGGGTGTGAA+AGG + chr4_1:61109215-61109234 None:intergenic 45.0%
TCCTCTTCCTCACAAATCCT+AGG - chr4_1:61109227-61109246 Msa0561540:CDS 45.0%
ACCTAGGATTTGTGAGGAAG+AGG + chr4_1:61109231-61109250 None:intergenic 45.0%
AGCAAGACCTAGGATTTGTG+AGG + chr4_1:61109237-61109256 None:intergenic 45.0%
TGGAGTGAAAAGCAAGACCT+AGG + chr4_1:61109247-61109266 None:intergenic 45.0%
!! ACTTTTTCTACTGCTTGCCG+TGG - chr4_1:61109963-61109982 Msa0561540:intron 45.0%
GCCTGCATAAGAAAACGAAG+TGG + chr4_1:61110807-61110826 None:intergenic 45.0%
!! GGAACGTTGATTTGGGTTTG+GGG - chr4_1:61110875-61110894 Msa0561540:intron 45.0%
! GACTCACGAGTATTTTCTCC+CGG - chr4_1:61111137-61111156 Msa0561540:intron 45.0%
! ACTCACGAGTATTTTCTCCC+GGG - chr4_1:61111138-61111157 Msa0561540:intron 45.0%
TGGAGGTAAAACGGCATCAA+AGG + chr4_1:61111301-61111320 None:intergenic 45.0%
ACTCGCACTTGGAGGTAAAA+CGG + chr4_1:61111310-61111329 None:intergenic 45.0%
TTCTTAGGGCAAACCTCAGA+GGG + chr4_1:61111366-61111385 None:intergenic 45.0%
ATTCTTAGGGCAAACCTCAG+AGG + chr4_1:61111367-61111386 None:intergenic 45.0%
!! AGTAGCATGAGTGTTGAAGC+CGG + chr4_1:61111466-61111485 None:intergenic 45.0%
TGCATTGTTGAGCCTAGACT+CGG - chr4_1:61111572-61111591 Msa0561540:intron 45.0%
!! CTTCTAGTTCATCCGAGTCT+AGG + chr4_1:61111587-61111606 None:intergenic 45.0%
AAGAAGAAGTCAGCACTGCA+AGG - chr4_1:61111616-61111635 Msa0561540:intron 45.0%
GTAACTGGAGCAAGTATCAG+AGG + chr4_1:61111664-61111683 None:intergenic 45.0%
CCTTGATTTCGTGCAGTAAC+TGG + chr4_1:61111679-61111698 None:intergenic 45.0%
CCAGTTACTGCACGAAATCA+AGG - chr4_1:61111676-61111695 Msa0561540:five_prime_UTR 45.0%
GCACGAAATCAAGGAAGAAG+AGG - chr4_1:61111685-61111704 Msa0561540:five_prime_UTR 45.0%
ACATAACTCGTCAGGGACAA+AGG - chr4_1:61111722-61111741 Msa0561540:five_prime_UTR 45.0%
CATAACTCGTCAGGGACAAA+GGG - chr4_1:61111723-61111742 Msa0561540:five_prime_UTR 45.0%
ACACTTGGAGATGAAGAGGA+TGG - chr4_1:61111770-61111789 Msa0561540:five_prime_UTR 45.0%
TTGAGAAATATCGTGCCTGG+TGG - chr4_1:61111801-61111820 Msa0561540:five_prime_UTR 45.0%
! AAACGGTAACGGTGTCCATT+TGG + chr4_1:61111833-61111852 None:intergenic 45.0%
! AATGGACACCGTTACCGTTT+TGG - chr4_1:61111833-61111852 Msa0561540:five_prime_UTR 45.0%
AGCTTCGTCCAAAACGGTAA+CGG + chr4_1:61111844-61111863 None:intergenic 45.0%
CTTCAACCTTGAGAGACTTG+AGG + chr4_1:61111875-61111894 None:intergenic 45.0%
TCACGCGTGCTCTAATCGAA+CGG - chr4_1:61108976-61108995 Msa0561540:CDS 50.0%
ACATGGATGATCTCAGCCGT+TGG + chr4_1:61109059-61109078 None:intergenic 50.0%
! GTGATGGGATGGGTCAAACA+TGG + chr4_1:61109076-61109095 None:intergenic 50.0%
! GATATGAAGGCGTGATGGGA+TGG + chr4_1:61109087-61109106 None:intergenic 50.0%
TTATCCTGTCTTGCTGCTGC+AGG - chr4_1:61110193-61110212 Msa0561540:intron 50.0%
TCTTGCTGCTGCAGGTGATT+AGG - chr4_1:61110201-61110220 Msa0561540:intron 50.0%
GCTGGATGGATGTTCCTACA+GGG + chr4_1:61111031-61111050 None:intergenic 50.0%
AGCTGGATGGATGTTCCTAC+AGG + chr4_1:61111032-61111051 None:intergenic 50.0%
! CGAGTATTTTCTCCCGGGAA+AGG - chr4_1:61111143-61111162 Msa0561540:intron 50.0%
AAAGGACGTACTGCCTTTCC+CGG + chr4_1:61111159-61111178 None:intergenic 50.0%
CCAATGGCTTCATCTGCCAA+AGG + chr4_1:61111177-61111196 None:intergenic 50.0%
CCTTTGGCAGATGAAGCCAT+TGG - chr4_1:61111174-61111193 Msa0561540:intron 50.0%
GCTCCACATGCTTCTGCTTT+CGG - chr4_1:61111210-61111229 Msa0561540:intron 50.0%
! GTACCGAAAGCAGAAGCATG+TGG + chr4_1:61111216-61111235 None:intergenic 50.0%
GTACCAAAGGCAGAACCATG+TGG + chr4_1:61111252-61111271 None:intergenic 50.0%
! GCATCAAAGGCAGAAGCATG+TGG + chr4_1:61111288-61111307 None:intergenic 50.0%
! GTTATCTGACTCGCACTTGG+AGG + chr4_1:61111318-61111337 None:intergenic 50.0%
! GGTGTTATCTGACTCGCACT+TGG + chr4_1:61111321-61111340 None:intergenic 50.0%
!! GTTTAACTTGCAGCCCTCTG+AGG - chr4_1:61111350-61111369 Msa0561540:intron 50.0%
ACTTGTAGGTCGTTGCAGGA+TGG + chr4_1:61111434-61111453 None:intergenic 50.0%
GCCGGGACTGTTAAACTTGT+AGG + chr4_1:61111448-61111467 None:intergenic 50.0%
!! GTAGCATGAGTGTTGAAGCC+GGG + chr4_1:61111465-61111484 None:intergenic 50.0%
TCATCCGCACATAACTCGTC+AGG - chr4_1:61111714-61111733 Msa0561540:five_prime_UTR 50.0%
CATCCGCACATAACTCGTCA+GGG - chr4_1:61111715-61111734 Msa0561540:five_prime_UTR 50.0%
TGTCCCTGACGAGTTATGTG+CGG + chr4_1:61111721-61111740 None:intergenic 50.0%
AGAAATATCGTGCCTGGTGG+AGG - chr4_1:61111804-61111823 Msa0561540:five_prime_UTR 50.0%
GAAGTCGAGCAATTCGGAGT+TGG - chr4_1:61111891-61111910 Msa0561540:five_prime_UTR 50.0%
ATCACCTGCAGCAGCAAGAC+AGG + chr4_1:61110200-61110219 None:intergenic 55.0%
TGCAGGTGATTAGGCTGCAG+AGG - chr4_1:61110210-61110229 Msa0561540:intron 55.0%
!! GCTGATTTGGCCTGTGCAAC+TGG + chr4_1:61111072-61111091 None:intergenic 55.0%
AAGGACGTACTGCCTTTCCC+GGG + chr4_1:61111158-61111177 None:intergenic 55.0%
GGGAAAGGCAGTACGTCCTT+TGG - chr4_1:61111158-61111177 Msa0561540:intron 55.0%
! GCTCCACATGGTTCTGCCTT+TGG - chr4_1:61111246-61111265 Msa0561540:intron 55.0%
TCCATTTGGTTGCCTCCACC+AGG + chr4_1:61111819-61111838 None:intergenic 55.0%
GCCTGGTGGAGGCAACCAAA+TGG - chr4_1:61111815-61111834 Msa0561540:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 61108974 61111917 61108974 ID=Msa0561540;Name=Msa0561540
chr4_1 mRNA 61108974 61111917 61108974 ID=Msa0561540-mRNA-1;Parent=Msa0561540;Name=Msa0561540-mRNA-1;_AED=0.08;_eAED=0.11;_QI=478|0.66|0.75|1|0|0|4|0|305
chr4_1 exon 61108974 61109810 61108974 ID=Msa0561540-mRNA-1:exon:19201;Parent=Msa0561540-mRNA-1
chr4_1 exon 61110532 61110676 61110532 ID=Msa0561540-mRNA-1:exon:19200;Parent=Msa0561540-mRNA-1
chr4_1 exon 61110984 61111119 61110984 ID=Msa0561540-mRNA-1:exon:19199;Parent=Msa0561540-mRNA-1
chr4_1 exon 61111643 61111917 61111643 ID=Msa0561540-mRNA-1:exon:19198;Parent=Msa0561540-mRNA-1
chr4_1 five_prime_UTR 61111643 61111917 61111643 ID=Msa0561540-mRNA-1:five_prime_utr;Parent=Msa0561540-mRNA-1
chr4_1 five_prime_UTR 61110984 61111119 61110984 ID=Msa0561540-mRNA-1:five_prime_utr;Parent=Msa0561540-mRNA-1
chr4_1 five_prime_UTR 61110610 61110676 61110610 ID=Msa0561540-mRNA-1:five_prime_utr;Parent=Msa0561540-mRNA-1
chr4_1 CDS 61110532 61110609 61110532 ID=Msa0561540-mRNA-1:cds;Parent=Msa0561540-mRNA-1
chr4_1 CDS 61108974 61109810 61108974 ID=Msa0561540-mRNA-1:cds;Parent=Msa0561540-mRNA-1
Gene Sequence

>Msa0561540

ATGTTCATTGCCAAAGTTGGGTACATTAAAATGAGTGTTGGAGTTATTGCATACAGTCAATTGATGTTGGTTTGCCAGGCCAAATCAGCATTGTTTGCGTTTAGTTTAGTGAGAGTGGACGGATATTTAATGCAGACTCACGAGTATTTTCTCCCGGGAAAGGCAGTACGTCCTTTGGCAGATGAAGCCATTGGCACTTATATGAATGCTCCACATGCTTCTGCTTTCGGTACTTACATGAATGCTCCACATGGTTCTGCCTTTGGTACTTATATGAATGCTCCACATGCTTCTGCCTTTGATGCCGTTTTACCTCCAAGTGCGAGTCAGATAACACCATTTGAAAGGTTTAACTTGCAGCCCTCTGAGGTTTGCCCTAAGAATTTCATTGTCTTTGATCAAACAGATCAACAAAACAGGATTTTGTTCCATCCTGCAACGACCTACAAGTTTAACAGTCCCGGCTTCAACACTCATGCTACTTGCACTCAAGATTTTGAGAAGGATAAAATTAATCAAATGGAAACGGAATTGTCATCTTCTTTTGTAGAGGACTCTAAGGACATTGATGCATTGTTGAGCCTAGACTCGGATGAACTAGAAGACTATGACGAAGAAGAAGTCAGCACTGCAAGGACTCATGAAAAAGATGATAGTACCTCTGATACTTGCTCCAGTTACTGCACGAAATCAAGGAAGAAGAGGTTGTTATCATCCGCACATAACTCGTCAGGGACAAAGGGTTACTGCATCAGTGAAAAGAAACAACACTTGGAGATGAAGAGGATGGTGAAAATATTGAGAAATATCGTGCCTGGTGGAGGCAACCAAATGGACACCGTTACCGTTTTGGACGAAGCTGTAAAATACCTCAAGTCTCTCAAGGTTGAAGTCGAGCAATTCGGAGTTGGACCG
Protein sequence

>Msa0561540

MFIAKVGYIKMSVGVIAYSQLMLVCQAKSALFAFSLVRVDGYLMQTHEYFLPGKAVRPLADEAIGTYMNAPHASAFGTYMNAPHGSAFGTYMNAPHASAFDAVLPPSASQITPFERFNLQPSEVCPKNFIVFDQTDQQNRILFHPATTYKFNSPGFNTHATCTQDFEKDKINQMETELSSSFVEDSKDIDALLSLDSDELEDYDEEEVSTARTHEKDDSTSDTCSSYCTKSRKKRLLSSAHNSSGTKGYCISEKKQHLEMKRMVKILRNIVPGGGNQMDTVTVLDEAVKYLKSLKVEVEQFGVGP