AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0715010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0715010 MtrunA17_Chr5g0402271 99.419 172 1 0 1 172 1 172 1.64e-124 347
Msa0715010 MtrunA17_Chr1g0190591 48.718 117 44 4 61 172 80 185 4.04e-24 93.2
Msa0715010 MtrunA17_Chr4g0044791 52.747 91 33 3 27 110 12 99 2.01e-23 90.1
Msa0715010 MtrunA17_Chr7g0274251 42.500 120 50 4 61 172 77 185 2.00e-19 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0715010 AT4G33985.1 52.381 147 54 4 29 171 14 148 1.91e-39 131
Msa0715010 AT3G50350.2 53.147 143 52 4 33 172 38 168 1.02e-37 127
Msa0715010 AT2G15590.2 46.358 151 58 6 29 172 11 145 7.54e-33 115
Msa0715010 AT3G50350.1 57.273 110 36 5 7 111 18 121 1.09e-28 105
Msa0715010 AT2G15590.1 55.056 89 28 4 29 110 11 94 6.79e-24 91.3
Msa0715010 AT1G08790.1 40.559 143 66 5 42 172 53 188 1.84e-19 81.3
Msa0715010 AT3G04700.1 39.456 147 69 3 42 172 48 190 2.48e-19 81.3
Msa0715010 AT2G31560.2 39.316 117 57 4 57 170 94 199 4.53e-17 75.5
Msa0715010 AT2G31560.1 39.316 117 57 4 57 170 94 199 4.53e-17 75.5
Msa0715010 AT2G31560.3 39.316 117 57 4 57 170 94 199 4.53e-17 75.5
Msa0715010 AT5G28690.1 38.255 149 68 5 42 172 49 191 5.71e-17 75.1
Msa0715010 AT5G28690.3 38.255 149 68 5 42 172 49 191 5.71e-17 75.1
Msa0715010 AT5G28690.2 38.255 149 68 5 42 172 49 191 5.71e-17 75.1
Msa0715010 AT1G05870.4 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT1G05870.12 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT1G05870.8 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT1G05870.1 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT1G05870.7 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT1G05870.2 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT1G05870.10 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT1G05870.5 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT1G05870.9 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT1G05870.3 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT1G05870.6 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT1G05870.11 38.983 118 57 4 57 170 80 186 7.63e-17 74.7
Msa0715010 AT2G15610.1 45.349 86 42 3 29 110 12 96 8.09e-17 73.6
Msa0715010 AT3G04710.3 34.595 185 100 4 1 169 8 187 1.22e-16 77.0
Msa0715010 AT2G43340.1 40.171 117 58 4 56 170 80 186 1.90e-16 73.6

Find 96 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGAGTGCAAAGATTTA+AGG 0.183003 5_1:+7884629 None:intergenic
CTCAAAGCTTGCATTGAATT+AGG 0.228374 5_1:-7884283 Msa0715010:CDS
GGAAGACGCTATGGCTTATT+AGG 0.235569 5_1:+7884555 None:intergenic
GTAGTAACGGCGGCGGATTT+AGG 0.271292 5_1:+7884148 None:intergenic
GCTTGCATTGAATTAGGTTT+TGG 0.295486 5_1:-7884277 Msa0715010:CDS
AACCTTGTCTTCACCGTTTG+TGG 0.303740 5_1:+7883770 None:intergenic
CTTTAGGTACCAAAACAATC+TGG 0.316908 5_1:+7883504 None:intergenic
ATAATAACATCAACTACTTT+AGG 0.332767 5_1:+7883488 None:intergenic
TAGTAACGGCGGCGGATTTA+GGG 0.354317 5_1:+7884149 None:intergenic
TAACCAAACCCAGATTGTTT+TGG 0.374396 5_1:-7883513 Msa0715010:three_prime_UTR
ATGATTATGCATGATGATTC+TGG 0.374745 5_1:-7883701 Msa0715010:three_prime_UTR
AGCCCTCGCACCGCCATCTT+CGG 0.378249 5_1:-7884073 Msa0715010:CDS
CGACGATCAGTTTCAACTTC+GGG 0.385163 5_1:+7884241 None:intergenic
GGCTCGTGGCCGGTTCTGTT+TGG 0.413415 5_1:+7883543 None:intergenic
GTACCAAAACAATCTGGGTT+TGG 0.415014 5_1:+7883510 None:intergenic
GGGGATTATTGATGATTAAC+CGG 0.415499 5_1:-7883651 Msa0715010:three_prime_UTR
ATAAAAGTTATAATGATTCT+CGG 0.416427 5_1:-7884174 Msa0715010:CDS
AATATTACCGTTGCCGAAGA+TGG 0.416533 5_1:+7884060 None:intergenic
GGTGGAGATGGCGGCGCGTA+GGG 0.419051 5_1:+7884478 None:intergenic
CTACACAATGAATACCAAAA+AGG 0.420369 5_1:+7883258 None:intergenic
GGCTTCGTCGTAAAGGAAAC+TGG 0.420518 5_1:-7884351 Msa0715010:CDS
AGAAAGGAAGGTGGTGGAGA+TGG 0.426640 5_1:+7884466 None:intergenic
TCTGGGTTTGGTTAAACTAA+AGG 0.430998 5_1:+7883522 None:intergenic
AGAAGAGTGCAAAGATTTAA+GGG 0.444563 5_1:+7884630 None:intergenic
ATTGTAACTTGTAACTTGTA+TGG 0.445013 5_1:+7883598 None:intergenic
TGGTGGAGATGGCGGCGCGT+AGG 0.463791 5_1:+7884477 None:intergenic
GCAATGATGATGAGCATGAA+TGG 0.476446 5_1:-7883673 Msa0715010:three_prime_UTR
TTGCCGAAGATGGCGGTGCG+AGG 0.483550 5_1:+7884070 None:intergenic
TGGAAGAGAAAGGAAGGTGG+TGG 0.489175 5_1:+7884460 None:intergenic
GCGTTGTTGTGCGGAGGTCG+AGG 0.489529 5_1:+7884514 None:intergenic
ATCTTTACCGTGACGAAGCG+TGG 0.490402 5_1:-7884372 Msa0715010:CDS
CGAGGGCTGCCACGAGGAGA+AGG 0.497711 5_1:+7884088 None:intergenic
GTTAAATTAATAACCATGGT+TGG 0.498910 5_1:+7883620 None:intergenic
TTTAGGTACCAAAACAATCT+GGG 0.503636 5_1:+7883505 None:intergenic
GAAGCGTGGCTTCGTCGTAA+AGG 0.505096 5_1:-7884358 Msa0715010:CDS
GTAAAGGAAACTGGAAGAAT+CGG 0.510681 5_1:-7884342 Msa0715010:CDS
CAATGATGATGAGCATGAAT+GGG 0.514334 5_1:-7883672 Msa0715010:three_prime_UTR
GAGTTCATCAACATCTTCAT+CGG 0.518800 5_1:+7884303 None:intergenic
GCCATCACGATCGGAGGCTG+CGG 0.520742 5_1:+7884114 None:intergenic
AGGGACCTCGCACGGTGCGT+TGG 0.523223 5_1:+7884534 None:intergenic
TGCCGAAGATGGCGGTGCGA+GGG 0.523267 5_1:+7884071 None:intergenic
GCTTCGTCACGGTAAAGATC+AGG 0.527383 5_1:+7884376 None:intergenic
CTCTCCTCAAGCAGCGATCG+TGG 0.533172 5_1:-7884402 Msa0715010:CDS
ATTTCACTACCAAACAGAAC+CGG 0.537637 5_1:-7883552 Msa0715010:three_prime_UTR
TGGTGGACGGTGGAAGAGAA+AGG 0.537926 5_1:+7884450 None:intergenic
ATCACGATCGGAGGCTGCGG+AGG 0.538111 5_1:+7884117 None:intergenic
GCATGATAAAGTTCAAGAGC+AGG 0.542994 5_1:+7884202 None:intergenic
TGGTATTCATTGTGTAGGTG+TGG 0.545424 5_1:-7883252 Msa0715010:three_prime_UTR
CGGTGCGTTGGAAGACGCTA+TGG 0.546409 5_1:+7884546 None:intergenic
GATTTAAGGGTGTGGTGAGG+TGG 0.547933 5_1:+7884643 None:intergenic
GGTGAGGTGGTAGATCAAGT+TGG 0.555238 5_1:+7884656 None:intergenic
ACGACGATCAGTTTCAACTT+CGG 0.557808 5_1:+7884240 None:intergenic
TATGGTTAAATTAATAACCA+TGG 0.559289 5_1:+7883616 None:intergenic
AGAAGGAGTGCCATCACGAT+CGG 0.561060 5_1:+7884105 None:intergenic
GTCTTCCAACGCACCGTGCG+AGG 0.563962 5_1:-7884539 Msa0715010:five_prime_UTR
TCGGAGGCTGCGGAGGAAGA+CGG 0.564577 5_1:+7884124 None:intergenic
ATTGATATGTTACTTGCTGT+CGG 0.564691 5_1:-7883467 Msa0715010:three_prime_UTR
GGACGGTGGAAGAGAAAGGA+AGG 0.564692 5_1:+7884454 None:intergenic
AATTCTTGCCTTAGCAGTGG+TGG 0.566176 5_1:+7884433 None:intergenic
CGCACCGCCATCTTCGGCAA+CGG 0.567388 5_1:-7884067 Msa0715010:intron
TTAAACTAAAGGCTCGTGGC+CGG 0.568803 5_1:+7883533 None:intergenic
GGCACAGGTTGTTGCTTGTG+CGG 0.568971 5_1:-7883738 Msa0715010:CDS
ATGCATGATGATTCTGGTGA+TGG 0.576855 5_1:-7883695 Msa0715010:three_prime_UTR
AAGGTTGAGACAGTGGGCAC+AGG 0.577432 5_1:-7883753 Msa0715010:CDS
TGAAGATGATGATCCACAAA+CGG 0.579168 5_1:-7883783 Msa0715010:intron
CGTTGTTGTGCGGAGGTCGA+GGG 0.580667 5_1:+7884515 None:intergenic
TCCACCGTCCACCACTGCTA+AGG 0.580926 5_1:-7884441 Msa0715010:CDS
CGGTGGAAGAGAAAGGAAGG+TGG 0.588878 5_1:+7884457 None:intergenic
CACGATCGCTGCTTGAGGAG+AGG 0.590516 5_1:+7884403 None:intergenic
AAGGAAGGTGGTGGAGATGG+CGG 0.597909 5_1:+7884469 None:intergenic
ATCCACAAACGGTGAAGACA+AGG 0.599536 5_1:-7883772 Msa0715010:CDS
AGTGCAAAGATTTAAGGGTG+TGG 0.601712 5_1:+7884635 None:intergenic
CTTGCCTTAGCAGTGGTGGA+CGG 0.601893 5_1:+7884437 None:intergenic
GGAGGAAGACGGTGTAGTAA+CGG 0.604938 5_1:+7884135 None:intergenic
GGAGGTCGAGGGACCTCGCA+CGG 0.605935 5_1:+7884526 None:intergenic
ATTACCGTTGCCGAAGATGG+CGG 0.608342 5_1:+7884063 None:intergenic
TCCGCAGCCTCCGATCGTGA+TGG 0.608934 5_1:-7884115 Msa0715010:CDS
GACGAAGCCACGCTTCGTCA+CGG 0.613832 5_1:+7884365 None:intergenic
ATCGGAGCGTTGTTGTGCGG+AGG 0.613915 5_1:+7884508 None:intergenic
GCGACCACGATCGCTGCTTG+AGG 0.614305 5_1:+7884398 None:intergenic
GACATCGGAGCGTTGTTGTG+CGG 0.617234 5_1:+7884505 None:intergenic
GCCTTAGCAGTGGTGGACGG+TGG 0.621956 5_1:+7884440 None:intergenic
TGAAGACAAGGTTGAGACAG+TGG 0.625033 5_1:-7883760 Msa0715010:CDS
GAGAATTCTTGCCTTAGCAG+TGG 0.625843 5_1:+7884430 None:intergenic
AGACGGTGTAGTAACGGCGG+CGG 0.627507 5_1:+7884141 None:intergenic
TTGGTTAAACTAAAGGCTCG+TGG 0.638119 5_1:+7883529 None:intergenic
GGCGCGTAGGGATCAGACAT+CGG 0.645725 5_1:+7884490 None:intergenic
GATGGCACTCCTTCTCCTCG+TGG 0.649473 5_1:-7884097 Msa0715010:CDS
GAAGACAAGGTTGAGACAGT+GGG 0.660668 5_1:-7883759 Msa0715010:CDS
AATGATGATGAGCATGAATG+GGG 0.660943 5_1:-7883671 Msa0715010:three_prime_UTR
AGGAGTGCCATCACGATCGG+AGG 0.666999 5_1:+7884108 None:intergenic
TATTGTCTAAAATGTGACTG+TGG 0.705865 5_1:-7883416 Msa0715010:three_prime_UTR
GGAAGACGGTGTAGTAACGG+CGG 0.724547 5_1:+7884138 None:intergenic
GCGGTGCGAGGGCTGCCACG+AGG 0.734065 5_1:+7884082 None:intergenic
ATGATGATGAGCATGAATGG+GGG 0.734195 5_1:-7883670 Msa0715010:three_prime_UTR
AAAGATTTAAGGGTGTGGTG+AGG 0.752540 5_1:+7884640 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAGTTATAATGATTCT+CGG - chr5_1:7883705-7883724 Msa0715010:exon 15.0%
!! ATAAAAGTTATAATGATTCT+CGG - chr5_1:7883705-7883724 Msa0715010:exon 15.0%
!!! TTTTTGTTTTGTTTTGGATT+CGG + chr5_1:7883293-7883312 None:intergenic 20.0%
!!! TTGGTTTTTTTGTTTTGTTT+TGG + chr5_1:7883299-7883318 None:intergenic 20.0%
!! TCATATTGAATTAAGATCTA+TGG - chr5_1:7884008-7884027 Msa0715010:intron 20.0%
!! TATGGTTAAATTAATAACCA+TGG + chr5_1:7884266-7884285 None:intergenic 20.0%
!! ATAATAACATCAACTACTTT+AGG + chr5_1:7884394-7884413 None:intergenic 20.0%
!!! TTTTTGTTTTGTTTTGGATT+CGG + chr5_1:7883293-7883312 None:intergenic 20.0%
!!! TTGGTTTTTTTGTTTTGTTT+TGG + chr5_1:7883299-7883318 None:intergenic 20.0%
!! TCATATTGAATTAAGATCTA+TGG - chr5_1:7884008-7884027 Msa0715010:intron 20.0%
!! TATGGTTAAATTAATAACCA+TGG + chr5_1:7884266-7884285 None:intergenic 20.0%
!! ATAATAACATCAACTACTTT+AGG + chr5_1:7884394-7884413 None:intergenic 20.0%
!!! TATGGCTTATTAGGTTTTTT+TGG + chr5_1:7883318-7883337 None:intergenic 25.0%
!! GTATTCTAAGAGTAATTAAG+AGG + chr5_1:7883912-7883931 None:intergenic 25.0%
! GTTAAATTAATAACCATGGT+TGG + chr5_1:7884262-7884281 None:intergenic 25.0%
!! ATTGTAACTTGTAACTTGTA+TGG + chr5_1:7884284-7884303 None:intergenic 25.0%
! TTAAAAATGTTACTACTACG+TGG - chr5_1:7884579-7884598 Msa0715010:five_prime_UTR 25.0%
!!! TATGGCTTATTAGGTTTTTT+TGG + chr5_1:7883318-7883337 None:intergenic 25.0%
!! GTATTCTAAGAGTAATTAAG+AGG + chr5_1:7883912-7883931 None:intergenic 25.0%
! GTTAAATTAATAACCATGGT+TGG + chr5_1:7884262-7884281 None:intergenic 25.0%
!! ATTGTAACTTGTAACTTGTA+TGG + chr5_1:7884284-7884303 None:intergenic 25.0%
! TTAAAAATGTTACTACTACG+TGG - chr5_1:7884579-7884598 Msa0715010:five_prime_UTR 25.0%
GACCAATAAAAAAGAGGTAA+GGG - chr5_1:7883197-7883216 Msa0715010:three_prime_UTR 30.0%
!!! AACCCTTACCTCTTTTTTAT+TGG + chr5_1:7883202-7883221 None:intergenic 30.0%
! AGAAGAGTGCAAAGATTTAA+GGG + chr5_1:7883252-7883271 None:intergenic 30.0%
! AAGAAGAGTGCAAAGATTTA+AGG + chr5_1:7883253-7883272 None:intergenic 30.0%
!! AAATTTTTGTTGACTGTGTC+CGG - chr5_1:7883954-7883973 Msa0715010:intron 30.0%
ATGATTATGCATGATGATTC+TGG - chr5_1:7884178-7884197 Msa0715010:CDS 30.0%
TTTAGGTACCAAAACAATCT+GGG + chr5_1:7884377-7884396 None:intergenic 30.0%
!! ATTGATATGTTACTTGCTGT+CGG - chr5_1:7884412-7884431 Msa0715010:CDS 30.0%
! TATTGTCTAAAATGTGACTG+TGG - chr5_1:7884463-7884482 Msa0715010:CDS 30.0%
! TTTTGTGTCAGAAAGAAAGA+CGG - chr5_1:7884552-7884571 Msa0715010:five_prime_UTR 30.0%
CTACACAATGAATACCAAAA+AGG + chr5_1:7884624-7884643 None:intergenic 30.0%
!! CTTTTTGGTATTCATTGTGT+AGG - chr5_1:7884622-7884641 Msa0715010:five_prime_UTR 30.0%
GACCAATAAAAAAGAGGTAA+GGG - chr5_1:7883197-7883216 Msa0715010:three_prime_UTR 30.0%
!!! AACCCTTACCTCTTTTTTAT+TGG + chr5_1:7883202-7883221 None:intergenic 30.0%
! AGAAGAGTGCAAAGATTTAA+GGG + chr5_1:7883252-7883271 None:intergenic 30.0%
! AAGAAGAGTGCAAAGATTTA+AGG + chr5_1:7883253-7883272 None:intergenic 30.0%
!! AAATTTTTGTTGACTGTGTC+CGG - chr5_1:7883954-7883973 Msa0715010:intron 30.0%
ATGATTATGCATGATGATTC+TGG - chr5_1:7884178-7884197 Msa0715010:CDS 30.0%
TTTAGGTACCAAAACAATCT+GGG + chr5_1:7884377-7884396 None:intergenic 30.0%
!! ATTGATATGTTACTTGCTGT+CGG - chr5_1:7884412-7884431 Msa0715010:CDS 30.0%
! TATTGTCTAAAATGTGACTG+TGG - chr5_1:7884463-7884482 Msa0715010:CDS 30.0%
! TTTTGTGTCAGAAAGAAAGA+CGG - chr5_1:7884552-7884571 Msa0715010:five_prime_UTR 30.0%
CTACACAATGAATACCAAAA+AGG + chr5_1:7884624-7884643 None:intergenic 30.0%
!! CTTTTTGGTATTCATTGTGT+AGG - chr5_1:7884622-7884641 Msa0715010:five_prime_UTR 30.0%
GGACCAATAAAAAAGAGGTA+AGG - chr5_1:7883196-7883215 Msa0715010:three_prime_UTR 35.0%
!!! TTGTTTTGTTTTGGATTCGG+CGG + chr5_1:7883290-7883309 None:intergenic 35.0%
GTAAAGGAAACTGGAAGAAT+CGG - chr5_1:7883537-7883556 Msa0715010:three_prime_UTR 35.0%
GAGTTCATCAACATCTTCAT+CGG + chr5_1:7883579-7883598 None:intergenic 35.0%
CTCAAAGCTTGCATTGAATT+AGG - chr5_1:7883596-7883615 Msa0715010:three_prime_UTR 35.0%
! GCTTGCATTGAATTAGGTTT+TGG - chr5_1:7883602-7883621 Msa0715010:three_prime_UTR 35.0%
TGAAGATGATGATCCACAAA+CGG - chr5_1:7884096-7884115 Msa0715010:CDS 35.0%
! CAATGATGATGAGCATGAAT+GGG - chr5_1:7884207-7884226 Msa0715010:CDS 35.0%
! AATGATGATGAGCATGAATG+GGG - chr5_1:7884208-7884227 Msa0715010:CDS 35.0%
! GGGGATTATTGATGATTAAC+CGG - chr5_1:7884228-7884247 Msa0715010:CDS 35.0%
ATTTCACTACCAAACAGAAC+CGG - chr5_1:7884327-7884346 Msa0715010:CDS 35.0%
! TCTGGGTTTGGTTAAACTAA+AGG + chr5_1:7884360-7884379 None:intergenic 35.0%
! TAACCAAACCCAGATTGTTT+TGG - chr5_1:7884366-7884385 Msa0715010:CDS 35.0%
CTTTAGGTACCAAAACAATC+TGG + chr5_1:7884378-7884397 None:intergenic 35.0%
GGACCAATAAAAAAGAGGTA+AGG - chr5_1:7883196-7883215 Msa0715010:three_prime_UTR 35.0%
!!! TTGTTTTGTTTTGGATTCGG+CGG + chr5_1:7883290-7883309 None:intergenic 35.0%
GTAAAGGAAACTGGAAGAAT+CGG - chr5_1:7883537-7883556 Msa0715010:three_prime_UTR 35.0%
GAGTTCATCAACATCTTCAT+CGG + chr5_1:7883579-7883598 None:intergenic 35.0%
CTCAAAGCTTGCATTGAATT+AGG - chr5_1:7883596-7883615 Msa0715010:three_prime_UTR 35.0%
! GCTTGCATTGAATTAGGTTT+TGG - chr5_1:7883602-7883621 Msa0715010:three_prime_UTR 35.0%
TGAAGATGATGATCCACAAA+CGG - chr5_1:7884096-7884115 Msa0715010:CDS 35.0%
! CAATGATGATGAGCATGAAT+GGG - chr5_1:7884207-7884226 Msa0715010:CDS 35.0%
! AATGATGATGAGCATGAATG+GGG - chr5_1:7884208-7884227 Msa0715010:CDS 35.0%
! GGGGATTATTGATGATTAAC+CGG - chr5_1:7884228-7884247 Msa0715010:CDS 35.0%
ATTTCACTACCAAACAGAAC+CGG - chr5_1:7884327-7884346 Msa0715010:CDS 35.0%
! TCTGGGTTTGGTTAAACTAA+AGG + chr5_1:7884360-7884379 None:intergenic 35.0%
! TAACCAAACCCAGATTGTTT+TGG - chr5_1:7884366-7884385 Msa0715010:CDS 35.0%
CTTTAGGTACCAAAACAATC+TGG + chr5_1:7884378-7884397 None:intergenic 35.0%
! AAAGATTTAAGGGTGTGGTG+AGG + chr5_1:7883242-7883261 None:intergenic 40.0%
AGTGCAAAGATTTAAGGGTG+TGG + chr5_1:7883247-7883266 None:intergenic 40.0%
!!! GATTCGGCGGTTTTTTGAAT+GGG + chr5_1:7883277-7883296 None:intergenic 40.0%
ACGACGATCAGTTTCAACTT+CGG + chr5_1:7883642-7883661 None:intergenic 40.0%
GCATGATAAAGTTCAAGAGC+AGG + chr5_1:7883680-7883699 None:intergenic 40.0%
AATATTACCGTTGCCGAAGA+TGG + chr5_1:7883822-7883841 None:intergenic 40.0%
ATGCATGATGATTCTGGTGA+TGG - chr5_1:7884184-7884203 Msa0715010:CDS 40.0%
! GCAATGATGATGAGCATGAA+TGG - chr5_1:7884206-7884225 Msa0715010:CDS 40.0%
! ATGATGATGAGCATGAATGG+GGG - chr5_1:7884209-7884228 Msa0715010:CDS 40.0%
! ACCATGGTTGGATTTTTCTC+CGG + chr5_1:7884250-7884269 None:intergenic 40.0%
TTGGTTAAACTAAAGGCTCG+TGG + chr5_1:7884353-7884372 None:intergenic 40.0%
GTACCAAAACAATCTGGGTT+TGG + chr5_1:7884372-7884391 None:intergenic 40.0%
AATACCAAAAAGGAGCAGCA+GGG + chr5_1:7884614-7884633 None:intergenic 40.0%
TGGTATTCATTGTGTAGGTG+TGG - chr5_1:7884627-7884646 Msa0715010:five_prime_UTR 40.0%
! AAAGATTTAAGGGTGTGGTG+AGG + chr5_1:7883242-7883261 None:intergenic 40.0%
AGTGCAAAGATTTAAGGGTG+TGG + chr5_1:7883247-7883266 None:intergenic 40.0%
!!! GATTCGGCGGTTTTTTGAAT+GGG + chr5_1:7883277-7883296 None:intergenic 40.0%
ACGACGATCAGTTTCAACTT+CGG + chr5_1:7883642-7883661 None:intergenic 40.0%
GCATGATAAAGTTCAAGAGC+AGG + chr5_1:7883680-7883699 None:intergenic 40.0%
AATATTACCGTTGCCGAAGA+TGG + chr5_1:7883822-7883841 None:intergenic 40.0%
ATGCATGATGATTCTGGTGA+TGG - chr5_1:7884184-7884203 Msa0715010:CDS 40.0%
! GCAATGATGATGAGCATGAA+TGG - chr5_1:7884206-7884225 Msa0715010:CDS 40.0%
! ATGATGATGAGCATGAATGG+GGG - chr5_1:7884209-7884228 Msa0715010:CDS 40.0%
! ACCATGGTTGGATTTTTCTC+CGG + chr5_1:7884250-7884269 None:intergenic 40.0%
TTGGTTAAACTAAAGGCTCG+TGG + chr5_1:7884353-7884372 None:intergenic 40.0%
GTACCAAAACAATCTGGGTT+TGG + chr5_1:7884372-7884391 None:intergenic 40.0%
AATACCAAAAAGGAGCAGCA+GGG + chr5_1:7884614-7884633 None:intergenic 40.0%
TGGTATTCATTGTGTAGGTG+TGG - chr5_1:7884627-7884646 Msa0715010:five_prime_UTR 40.0%
!!! GGATTCGGCGGTTTTTTGAA+TGG + chr5_1:7883278-7883297 None:intergenic 45.0%
GGAAGACGCTATGGCTTATT+AGG + chr5_1:7883327-7883346 None:intergenic 45.0%
AATTCTTGCCTTAGCAGTGG+TGG + chr5_1:7883449-7883468 None:intergenic 45.0%
GAGAATTCTTGCCTTAGCAG+TGG + chr5_1:7883452-7883471 None:intergenic 45.0%
CGACGATCAGTTTCAACTTC+GGG + chr5_1:7883641-7883660 None:intergenic 45.0%
TGAAGCACAAACACAGACAC+CGG + chr5_1:7883976-7883995 None:intergenic 45.0%
ATCCACAAACGGTGAAGACA+AGG - chr5_1:7884107-7884126 Msa0715010:CDS 45.0%
AACCTTGTCTTCACCGTTTG+TGG + chr5_1:7884112-7884131 None:intergenic 45.0%
TGAAGACAAGGTTGAGACAG+TGG - chr5_1:7884119-7884138 Msa0715010:CDS 45.0%
GAAGACAAGGTTGAGACAGT+GGG - chr5_1:7884120-7884139 Msa0715010:CDS 45.0%
ACCGGAGAAAAATCCAACCA+TGG - chr5_1:7884246-7884265 Msa0715010:CDS 45.0%
TTAAACTAAAGGCTCGTGGC+CGG + chr5_1:7884349-7884368 None:intergenic 45.0%
ATACCAAAAAGGAGCAGCAG+GGG + chr5_1:7884613-7884632 None:intergenic 45.0%
GAATACCAAAAAGGAGCAGC+AGG + chr5_1:7884615-7884634 None:intergenic 45.0%
!!! GGATTCGGCGGTTTTTTGAA+TGG + chr5_1:7883278-7883297 None:intergenic 45.0%
GGAAGACGCTATGGCTTATT+AGG + chr5_1:7883327-7883346 None:intergenic 45.0%
AATTCTTGCCTTAGCAGTGG+TGG + chr5_1:7883449-7883468 None:intergenic 45.0%
GAGAATTCTTGCCTTAGCAG+TGG + chr5_1:7883452-7883471 None:intergenic 45.0%
CGACGATCAGTTTCAACTTC+GGG + chr5_1:7883641-7883660 None:intergenic 45.0%
TGAAGCACAAACACAGACAC+CGG + chr5_1:7883976-7883995 None:intergenic 45.0%
ATCCACAAACGGTGAAGACA+AGG - chr5_1:7884107-7884126 Msa0715010:CDS 45.0%
AACCTTGTCTTCACCGTTTG+TGG + chr5_1:7884112-7884131 None:intergenic 45.0%
TGAAGACAAGGTTGAGACAG+TGG - chr5_1:7884119-7884138 Msa0715010:CDS 45.0%
GAAGACAAGGTTGAGACAGT+GGG - chr5_1:7884120-7884139 Msa0715010:CDS 45.0%
ACCGGAGAAAAATCCAACCA+TGG - chr5_1:7884246-7884265 Msa0715010:CDS 45.0%
TTAAACTAAAGGCTCGTGGC+CGG + chr5_1:7884349-7884368 None:intergenic 45.0%
ATACCAAAAAGGAGCAGCAG+GGG + chr5_1:7884613-7884632 None:intergenic 45.0%
GAATACCAAAAAGGAGCAGC+AGG + chr5_1:7884615-7884634 None:intergenic 45.0%
GGTGAGGTGGTAGATCAAGT+TGG + chr5_1:7883226-7883245 None:intergenic 50.0%
! GATTTAAGGGTGTGGTGAGG+TGG + chr5_1:7883239-7883258 None:intergenic 50.0%
!! AGAAAGGAAGGTGGTGGAGA+TGG + chr5_1:7883416-7883435 None:intergenic 50.0%
TGGAAGAGAAAGGAAGGTGG+TGG + chr5_1:7883422-7883441 None:intergenic 50.0%
GCTTCGTCACGGTAAAGATC+AGG + chr5_1:7883506-7883525 None:intergenic 50.0%
ATCTTTACCGTGACGAAGCG+TGG - chr5_1:7883507-7883526 Msa0715010:three_prime_UTR 50.0%
GGCTTCGTCGTAAAGGAAAC+TGG - chr5_1:7883528-7883547 Msa0715010:three_prime_UTR 50.0%
! TAGTAACGGCGGCGGATTTA+GGG + chr5_1:7883733-7883752 None:intergenic 50.0%
GGAGGAAGACGGTGTAGTAA+CGG + chr5_1:7883747-7883766 None:intergenic 50.0%
!! AGAAGGAGTGCCATCACGAT+CGG + chr5_1:7883777-7883796 None:intergenic 50.0%
ATTACCGTTGCCGAAGATGG+CGG + chr5_1:7883819-7883838 None:intergenic 50.0%
! AATCCCCTGCTGCTCCTTTT+TGG - chr5_1:7884607-7884626 Msa0715010:five_prime_UTR 50.0%
GGTGAGGTGGTAGATCAAGT+TGG + chr5_1:7883226-7883245 None:intergenic 50.0%
! GATTTAAGGGTGTGGTGAGG+TGG + chr5_1:7883239-7883258 None:intergenic 50.0%
!! AGAAAGGAAGGTGGTGGAGA+TGG + chr5_1:7883416-7883435 None:intergenic 50.0%
TGGAAGAGAAAGGAAGGTGG+TGG + chr5_1:7883422-7883441 None:intergenic 50.0%
GCTTCGTCACGGTAAAGATC+AGG + chr5_1:7883506-7883525 None:intergenic 50.0%
ATCTTTACCGTGACGAAGCG+TGG - chr5_1:7883507-7883526 Msa0715010:three_prime_UTR 50.0%
GGCTTCGTCGTAAAGGAAAC+TGG - chr5_1:7883528-7883547 Msa0715010:three_prime_UTR 50.0%
! TAGTAACGGCGGCGGATTTA+GGG + chr5_1:7883733-7883752 None:intergenic 50.0%
GGAGGAAGACGGTGTAGTAA+CGG + chr5_1:7883747-7883766 None:intergenic 50.0%
!! AGAAGGAGTGCCATCACGAT+CGG + chr5_1:7883777-7883796 None:intergenic 50.0%
ATTACCGTTGCCGAAGATGG+CGG + chr5_1:7883819-7883838 None:intergenic 50.0%
! AATCCCCTGCTGCTCCTTTT+TGG - chr5_1:7884607-7884626 Msa0715010:five_prime_UTR 50.0%
!! GACATCGGAGCGTTGTTGTG+CGG + chr5_1:7883377-7883396 None:intergenic 55.0%
!! AAGGAAGGTGGTGGAGATGG+CGG + chr5_1:7883413-7883432 None:intergenic 55.0%
CGGTGGAAGAGAAAGGAAGG+TGG + chr5_1:7883425-7883444 None:intergenic 55.0%
GGACGGTGGAAGAGAAAGGA+AGG + chr5_1:7883428-7883447 None:intergenic 55.0%
! TGGTGGACGGTGGAAGAGAA+AGG + chr5_1:7883432-7883451 None:intergenic 55.0%
! CTTGCCTTAGCAGTGGTGGA+CGG + chr5_1:7883445-7883464 None:intergenic 55.0%
GAAGCGTGGCTTCGTCGTAA+AGG - chr5_1:7883521-7883540 Msa0715010:three_prime_UTR 55.0%
! GTAGTAACGGCGGCGGATTT+AGG + chr5_1:7883734-7883753 None:intergenic 55.0%
GGAAGACGGTGTAGTAACGG+CGG + chr5_1:7883744-7883763 None:intergenic 55.0%
AAGGTTGAGACAGTGGGCAC+AGG - chr5_1:7884126-7884145 Msa0715010:CDS 55.0%
! GGCACAGGTTGTTGCTTGTG+CGG - chr5_1:7884141-7884160 Msa0715010:CDS 55.0%
!! GACATCGGAGCGTTGTTGTG+CGG + chr5_1:7883377-7883396 None:intergenic 55.0%
!! AAGGAAGGTGGTGGAGATGG+CGG + chr5_1:7883413-7883432 None:intergenic 55.0%
CGGTGGAAGAGAAAGGAAGG+TGG + chr5_1:7883425-7883444 None:intergenic 55.0%
GGACGGTGGAAGAGAAAGGA+AGG + chr5_1:7883428-7883447 None:intergenic 55.0%
! TGGTGGACGGTGGAAGAGAA+AGG + chr5_1:7883432-7883451 None:intergenic 55.0%
! CTTGCCTTAGCAGTGGTGGA+CGG + chr5_1:7883445-7883464 None:intergenic 55.0%
GAAGCGTGGCTTCGTCGTAA+AGG - chr5_1:7883521-7883540 Msa0715010:three_prime_UTR 55.0%
! GTAGTAACGGCGGCGGATTT+AGG + chr5_1:7883734-7883753 None:intergenic 55.0%
GGAAGACGGTGTAGTAACGG+CGG + chr5_1:7883744-7883763 None:intergenic 55.0%
AAGGTTGAGACAGTGGGCAC+AGG - chr5_1:7884126-7884145 Msa0715010:CDS 55.0%
! GGCACAGGTTGTTGCTTGTG+CGG - chr5_1:7884141-7884160 Msa0715010:CDS 55.0%
CGGTGCGTTGGAAGACGCTA+TGG + chr5_1:7883336-7883355 None:intergenic 60.0%
CGTTGTTGTGCGGAGGTCGA+GGG + chr5_1:7883367-7883386 None:intergenic 60.0%
!! ATCGGAGCGTTGTTGTGCGG+AGG + chr5_1:7883374-7883393 None:intergenic 60.0%
GGCGCGTAGGGATCAGACAT+CGG + chr5_1:7883392-7883411 None:intergenic 60.0%
TCCACCGTCCACCACTGCTA+AGG - chr5_1:7883438-7883457 Msa0715010:three_prime_UTR 60.0%
CACGATCGCTGCTTGAGGAG+AGG + chr5_1:7883479-7883498 None:intergenic 60.0%
CTCTCCTCAAGCAGCGATCG+TGG - chr5_1:7883477-7883496 Msa0715010:three_prime_UTR 60.0%
GACGAAGCCACGCTTCGTCA+CGG + chr5_1:7883517-7883536 None:intergenic 60.0%
AGACGGTGTAGTAACGGCGG+CGG + chr5_1:7883741-7883760 None:intergenic 60.0%
!! AGGAGTGCCATCACGATCGG+AGG + chr5_1:7883774-7883793 None:intergenic 60.0%
GATGGCACTCCTTCTCCTCG+TGG - chr5_1:7883782-7883801 Msa0715010:intron 60.0%
CGGTGCGTTGGAAGACGCTA+TGG + chr5_1:7883336-7883355 None:intergenic 60.0%
CGTTGTTGTGCGGAGGTCGA+GGG + chr5_1:7883367-7883386 None:intergenic 60.0%
!! ATCGGAGCGTTGTTGTGCGG+AGG + chr5_1:7883374-7883393 None:intergenic 60.0%
GGCGCGTAGGGATCAGACAT+CGG + chr5_1:7883392-7883411 None:intergenic 60.0%
TCCACCGTCCACCACTGCTA+AGG - chr5_1:7883438-7883457 Msa0715010:three_prime_UTR 60.0%
CACGATCGCTGCTTGAGGAG+AGG + chr5_1:7883479-7883498 None:intergenic 60.0%
CTCTCCTCAAGCAGCGATCG+TGG - chr5_1:7883477-7883496 Msa0715010:three_prime_UTR 60.0%
GACGAAGCCACGCTTCGTCA+CGG + chr5_1:7883517-7883536 None:intergenic 60.0%
AGACGGTGTAGTAACGGCGG+CGG + chr5_1:7883741-7883760 None:intergenic 60.0%
!! AGGAGTGCCATCACGATCGG+AGG + chr5_1:7883774-7883793 None:intergenic 60.0%
GATGGCACTCCTTCTCCTCG+TGG - chr5_1:7883782-7883801 Msa0715010:intron 60.0%
GTCTTCCAACGCACCGTGCG+AGG - chr5_1:7883340-7883359 Msa0715010:three_prime_UTR 65.0%
GCGTTGTTGTGCGGAGGTCG+AGG + chr5_1:7883368-7883387 None:intergenic 65.0%
!! GCCTTAGCAGTGGTGGACGG+TGG + chr5_1:7883442-7883461 None:intergenic 65.0%
GCGACCACGATCGCTGCTTG+AGG + chr5_1:7883484-7883503 None:intergenic 65.0%
TCGGAGGCTGCGGAGGAAGA+CGG + chr5_1:7883758-7883777 None:intergenic 65.0%
ATCACGATCGGAGGCTGCGG+AGG + chr5_1:7883765-7883784 None:intergenic 65.0%
TCCGCAGCCTCCGATCGTGA+TGG - chr5_1:7883764-7883783 Msa0715010:CDS 65.0%
GCCATCACGATCGGAGGCTG+CGG + chr5_1:7883768-7883787 None:intergenic 65.0%
AGCCCTCGCACCGCCATCTT+CGG - chr5_1:7883806-7883825 Msa0715010:intron 65.0%
! TGCCGAAGATGGCGGTGCGA+GGG + chr5_1:7883811-7883830 None:intergenic 65.0%
! TTGCCGAAGATGGCGGTGCG+AGG + chr5_1:7883812-7883831 None:intergenic 65.0%
CGCACCGCCATCTTCGGCAA+CGG - chr5_1:7883812-7883831 Msa0715010:intron 65.0%
! GGCTCGTGGCCGGTTCTGTT+TGG + chr5_1:7884339-7884358 None:intergenic 65.0%
GTCTTCCAACGCACCGTGCG+AGG - chr5_1:7883340-7883359 Msa0715010:three_prime_UTR 65.0%
GCGTTGTTGTGCGGAGGTCG+AGG + chr5_1:7883368-7883387 None:intergenic 65.0%
!! GCCTTAGCAGTGGTGGACGG+TGG + chr5_1:7883442-7883461 None:intergenic 65.0%
GCGACCACGATCGCTGCTTG+AGG + chr5_1:7883484-7883503 None:intergenic 65.0%
TCGGAGGCTGCGGAGGAAGA+CGG + chr5_1:7883758-7883777 None:intergenic 65.0%
ATCACGATCGGAGGCTGCGG+AGG + chr5_1:7883765-7883784 None:intergenic 65.0%
TCCGCAGCCTCCGATCGTGA+TGG - chr5_1:7883764-7883783 Msa0715010:CDS 65.0%
GCCATCACGATCGGAGGCTG+CGG + chr5_1:7883768-7883787 None:intergenic 65.0%
AGCCCTCGCACCGCCATCTT+CGG - chr5_1:7883806-7883825 Msa0715010:intron 65.0%
! TGCCGAAGATGGCGGTGCGA+GGG + chr5_1:7883811-7883830 None:intergenic 65.0%
! TTGCCGAAGATGGCGGTGCG+AGG + chr5_1:7883812-7883831 None:intergenic 65.0%
CGCACCGCCATCTTCGGCAA+CGG - chr5_1:7883812-7883831 Msa0715010:intron 65.0%
! GGCTCGTGGCCGGTTCTGTT+TGG + chr5_1:7884339-7884358 None:intergenic 65.0%
AGGGACCTCGCACGGTGCGT+TGG + chr5_1:7883348-7883367 None:intergenic 70.0%
GGAGGTCGAGGGACCTCGCA+CGG + chr5_1:7883356-7883375 None:intergenic 70.0%
! GGTGGAGATGGCGGCGCGTA+GGG + chr5_1:7883404-7883423 None:intergenic 70.0%
! TGGTGGAGATGGCGGCGCGT+AGG + chr5_1:7883405-7883424 None:intergenic 70.0%
CGAGGGCTGCCACGAGGAGA+AGG + chr5_1:7883794-7883813 None:intergenic 70.0%
AGGGACCTCGCACGGTGCGT+TGG + chr5_1:7883348-7883367 None:intergenic 70.0%
GGAGGTCGAGGGACCTCGCA+CGG + chr5_1:7883356-7883375 None:intergenic 70.0%
! GGTGGAGATGGCGGCGCGTA+GGG + chr5_1:7883404-7883423 None:intergenic 70.0%
! TGGTGGAGATGGCGGCGCGT+AGG + chr5_1:7883405-7883424 None:intergenic 70.0%
CGAGGGCTGCCACGAGGAGA+AGG + chr5_1:7883794-7883813 None:intergenic 70.0%
! GCGGTGCGAGGGCTGCCACG+AGG + chr5_1:7883800-7883819 None:intergenic 80.0%
! GCGGTGCGAGGGCTGCCACG+AGG + chr5_1:7883800-7883819 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 7883194 7884707 7883194 ID=Msa0715010;Name=Msa0715010
chr5_1 mRNA 7883194 7884707 7883194 ID=Msa0715010-mRNA-1;Parent=Msa0715010;Name=Msa0715010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=198|1|1|1|1|1|2|530|172
chr5_1 exon 7883194 7883800 7883194 ID=Msa0715010-mRNA-1:exon:3269;Parent=Msa0715010-mRNA-1
chr5_1 exon 7884068 7884707 7884068 ID=Msa0715010-mRNA-1:exon:3268;Parent=Msa0715010-mRNA-1
chr5_1 five_prime_UTR 7884510 7884707 7884510 ID=Msa0715010-mRNA-1:five_prime_utr;Parent=Msa0715010-mRNA-1
chr5_1 CDS 7884068 7884509 7884068 ID=Msa0715010-mRNA-1:cds;Parent=Msa0715010-mRNA-1
chr5_1 CDS 7883724 7883800 7883724 ID=Msa0715010-mRNA-1:cds;Parent=Msa0715010-mRNA-1
chr5_1 three_prime_UTR 7883194 7883723 7883194 ID=Msa0715010-mRNA-1:three_prime_utr;Parent=Msa0715010-mRNA-1
Gene Sequence

>Msa0715010

ATGTCTGATCCCTACGCGCCGCCATCTCCACCACCTTCCTTTCTCTTCCACCGTCCACCACTGCTAAGGCAAGAATTCTCTTCTCCTCTCCTCAAGCAGCGATCGTGGTCGCCTGATCTTTACCGTGACGAAGCGTGGCTTCGTCGTAAAGGAAACTGGAAGAATCGGAGAAGTAAAAGCGTTACCGATGAAGATGTTGATGAACTCAAAGCTTGCATTGAATTAGGTTTTGGATTTGAATCTTCACCCGAAGTTGAAACTGATCGTCGTCTTGTTGATACTTTACCTGCTCTTGAACTTTATCATGCTGTTAATAAAAGTTATAATGATTCTCGGAACCCTAAATCCGCCGCCGTTACTACACCGTCTTCCTCCGCAGCCTCCGATCGTGATGGCACTCCTTCTCCTCGTGGCAGCCCTCGCACCGCCATCTTCGGCAACGATGATGATCCACAAACGGTGAAGACAAGGTTGAGACAGTGGGCACAGGTTGTTGCTTGTGCGGTTCGTCAAAGCTGA
Protein sequence

>Msa0715010

MSDPYAPPSPPPSFLFHRPPLLRQEFSSPLLKQRSWSPDLYRDEAWLRRKGNWKNRRSKSVTDEDVDELKACIELGFGFESSPEVETDRRLVDTLPALELYHAVNKSYNDSRNPKSAAVTTPSSSAASDRDGTPSPRGSPRTAIFGNDDDPQTVKTRLRQWAQVVACAVRQS*