Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0761020 | G7KF23 | 97.500 | 120 | 3 | 0 | 1 | 120 | 1 | 120 | 1.96e-77 | 233 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0708680 | Msa0761020 | 0.814371 | 1.593830e-51 | -8.615850e-47 |
| Msa0720490 | Msa0761020 | 0.816598 | 5.091212e-52 | -8.615850e-47 |
| Msa0761020 | Msa0803310 | 0.818863 | 1.570022e-52 | -8.615850e-47 |
| Msa0761020 | Msa0841670 | 0.859112 | 5.179161e-63 | -8.615850e-47 |
| Msa0761020 | Msa0896920 | 0.809936 | 1.478866e-50 | -8.615850e-47 |
| Msa0761020 | Msa1191350 | 0.804743 | 1.865788e-49 | -8.615850e-47 |
| Msa0761020 | Msa1319030 | 0.803768 | 2.977316e-49 | -8.615850e-47 |
| Msa0761020 | Msa1319190 | 0.812214 | 4.745316e-51 | -8.615850e-47 |
| Msa0170990 | Msa0761020 | 0.809203 | 2.124913e-50 | -8.615850e-47 |
| Msa0374040 | Msa0761020 | 0.807376 | 5.210996e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0761020 | MtrunA17_Chr5g0411241 | 97.500 | 120 | 3 | 0 | 1 | 120 | 1 | 120 | 3.78e-81 | 233 |
| Msa0761020 | MtrunA17_Chr5g0411231 | 83.333 | 120 | 20 | 0 | 1 | 120 | 1 | 120 | 2.98e-68 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGCCTTCAGTCTTGAAAA+TGG | 0.356723 | 5_2:+18011327 | None:intergenic |
| GCAAAATCTTTGCCAGCATC+AGG | 0.390660 | 5_2:+18011813 | None:intergenic |
| TTATGCAGTGATGGTGGAGC+TGG | 0.397441 | 5_2:-18011424 | Msa0761020:intron |
| CTTGCTCTTGAGTGAAGCTC+TGG | 0.411535 | 5_2:+18012104 | None:intergenic |
| TCACTGCTACTCTCAATAAC+TGG | 0.438274 | 5_2:+18011397 | None:intergenic |
| CCCTTGCCAGAGTTTACACC+AGG | 0.459349 | 5_2:+18011307 | None:intergenic |
| AGGACTGAAGGGCCTGATGC+TGG | 0.490624 | 5_2:-18011825 | Msa0761020:CDS |
| GGCAATGTGTTGTGCAAGAC+AGG | 0.514042 | 5_2:-18011873 | Msa0761020:CDS |
| ACAACACATTGCCCAAGCCC+TGG | 0.525921 | 5_2:+18011883 | None:intergenic |
| CCCTGGTGTAAACTCTGGCA+AGG | 0.541663 | 5_2:-18011308 | Msa0761020:CDS |
| TATGCAGTGATGGTGGAGCT+GGG | 0.557903 | 5_2:-18011423 | Msa0761020:intron |
| ACATTCTGCAAGTGTGGTGA+AGG | 0.561546 | 5_2:-18011361 | Msa0761020:CDS |
| ATTGAGAGTAGCAGTGACAA+TGG | 0.563224 | 5_2:-18011391 | Msa0761020:CDS |
| CAGAGCTTCACTCAAGAGCA+AGG | 0.567749 | 5_2:-18012103 | Msa0761020:CDS |
| TTGCTCTTGAGTGAAGCTCT+GGG | 0.573531 | 5_2:+18012105 | None:intergenic |
| GTTGGACATGTGTTATAACT+AGG | 0.581816 | 5_2:-18011845 | Msa0761020:CDS |
| GTGTTATAACTAGGACTGAA+GGG | 0.595455 | 5_2:-18011836 | Msa0761020:CDS |
| TGTGCAAGACAGGTAAAGGT+TGG | 0.598871 | 5_2:-18011863 | Msa0761020:CDS |
| AAACTCTTATGCAGTGATGG+TGG | 0.602169 | 5_2:-18011430 | Msa0761020:intron |
| GAAGGCCCTGGTGTAAACTC+TGG | 0.603912 | 5_2:-18011313 | Msa0761020:CDS |
| TGTGTTATAACTAGGACTGA+AGG | 0.610701 | 5_2:-18011837 | Msa0761020:CDS |
| CCTGGTGTAAACTCTGGCAA+GGG | 0.620143 | 5_2:-18011307 | Msa0761020:CDS |
| GTGTTGTGCAAGACAGGTAA+AGG | 0.626364 | 5_2:-18011867 | Msa0761020:CDS |
| AGAGCTTCACTCAAGAGCAA+GGG | 0.651871 | 5_2:-18012102 | Msa0761020:intron |
| TCTGCAAGTGTGGTGAAGGG+TGG | 0.652623 | 5_2:-18011357 | Msa0761020:CDS |
| AGTGAAACATTCTGCAAGTG+TGG | 0.671634 | 5_2:-18011367 | Msa0761020:CDS |
| CATTCTGCAAGTGTGGTGAA+GGG | 0.681189 | 5_2:-18011360 | Msa0761020:CDS |
| CCTTGCCAGAGTTTACACCA+GGG | 0.773669 | 5_2:+18011308 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACTGTAAAGATGAAATAA+AGG | + | chr5_2:18011326-18011345 | None:intergenic | 20.0% |
| !! | AAACTGTAAAGATGAAATAA+AGG | + | chr5_2:18011326-18011345 | None:intergenic | 20.0% |
| ! | TGTAAAGATGAAATAAAGGA+TGG | + | chr5_2:18011322-18011341 | None:intergenic | 25.0% |
| ! | AATTATGACAGGTATGAAAA+TGG | + | chr5_2:18011420-18011439 | None:intergenic | 25.0% |
| !! | TTTCTAACATTTTCATAACC+GGG | + | chr5_2:18011797-18011816 | None:intergenic | 25.0% |
| !! | GTTTCTAACATTTTCATAAC+CGG | + | chr5_2:18011798-18011817 | None:intergenic | 25.0% |
| ! | TGTAAAGATGAAATAAAGGA+TGG | + | chr5_2:18011322-18011341 | None:intergenic | 25.0% |
| ! | AATTATGACAGGTATGAAAA+TGG | + | chr5_2:18011420-18011439 | None:intergenic | 25.0% |
| !! | TTTCTAACATTTTCATAACC+GGG | + | chr5_2:18011797-18011816 | None:intergenic | 25.0% |
| !! | GTTTCTAACATTTTCATAAC+CGG | + | chr5_2:18011798-18011817 | None:intergenic | 25.0% |
| ! | GCTTATTGGTTAATTATGAC+AGG | + | chr5_2:18011431-18011450 | None:intergenic | 30.0% |
| ! | TTAACCAATAAGCTTTGTGT+AGG | - | chr5_2:18011438-18011457 | Msa0761020:intron | 30.0% |
| TGAAAATGTCAAATACTGCT+TGG | + | chr5_2:18011708-18011727 | None:intergenic | 30.0% | |
| ! | TTCTAACATTTTCATAACCG+GGG | + | chr5_2:18011796-18011815 | None:intergenic | 30.0% |
| ! | GCTTATTGGTTAATTATGAC+AGG | + | chr5_2:18011431-18011450 | None:intergenic | 30.0% |
| ! | TTAACCAATAAGCTTTGTGT+AGG | - | chr5_2:18011438-18011457 | Msa0761020:intron | 30.0% |
| TGAAAATGTCAAATACTGCT+TGG | + | chr5_2:18011708-18011727 | None:intergenic | 30.0% | |
| ! | TTCTAACATTTTCATAACCG+GGG | + | chr5_2:18011796-18011815 | None:intergenic | 30.0% |
| ! | GAAAATGGAGCGCATTATTT+TGG | + | chr5_2:18011405-18011424 | None:intergenic | 35.0% |
| CTTTCCTACACAAAGCTTAT+TGG | + | chr5_2:18011445-18011464 | None:intergenic | 35.0% | |
| ! | CCTGAGTTTTTATCTTGAAC+TGG | + | chr5_2:18011470-18011489 | None:intergenic | 35.0% |
| CCAGTTCAAGATAAAAACTC+AGG | - | chr5_2:18011467-18011486 | Msa0761020:intron | 35.0% | |
| GTTGGACATGTGTTATAACT+AGG | - | chr5_2:18011528-18011547 | Msa0761020:intron | 35.0% | |
| TGTGTTATAACTAGGACTGA+AGG | - | chr5_2:18011536-18011555 | Msa0761020:intron | 35.0% | |
| GTGTTATAACTAGGACTGAA+GGG | - | chr5_2:18011537-18011556 | Msa0761020:intron | 35.0% | |
| ! | GGCAAAGATTTTGCTAAATG+TGG | - | chr5_2:18011569-18011588 | Msa0761020:intron | 35.0% |
| ! | TCTAACATTTTCATAACCGG+GGG | + | chr5_2:18011795-18011814 | None:intergenic | 35.0% |
| ATCAAACTCTTATGCAGTGA+TGG | - | chr5_2:18011940-18011959 | Msa0761020:intron | 35.0% | |
| !! | TGTTCCATTTTCAAGACTGA+AGG | - | chr5_2:18012042-18012061 | Msa0761020:intron | 35.0% |
| ! | GAAAATGGAGCGCATTATTT+TGG | + | chr5_2:18011405-18011424 | None:intergenic | 35.0% |
| CTTTCCTACACAAAGCTTAT+TGG | + | chr5_2:18011445-18011464 | None:intergenic | 35.0% | |
| ! | CCTGAGTTTTTATCTTGAAC+TGG | + | chr5_2:18011470-18011489 | None:intergenic | 35.0% |
| CCAGTTCAAGATAAAAACTC+AGG | - | chr5_2:18011467-18011486 | Msa0761020:intron | 35.0% | |
| GTTGGACATGTGTTATAACT+AGG | - | chr5_2:18011528-18011547 | Msa0761020:intron | 35.0% | |
| TGTGTTATAACTAGGACTGA+AGG | - | chr5_2:18011536-18011555 | Msa0761020:intron | 35.0% | |
| GTGTTATAACTAGGACTGAA+GGG | - | chr5_2:18011537-18011556 | Msa0761020:intron | 35.0% | |
| ! | GGCAAAGATTTTGCTAAATG+TGG | - | chr5_2:18011569-18011588 | Msa0761020:intron | 35.0% |
| ! | TCTAACATTTTCATAACCGG+GGG | + | chr5_2:18011795-18011814 | None:intergenic | 35.0% |
| ATCAAACTCTTATGCAGTGA+TGG | - | chr5_2:18011940-18011959 | Msa0761020:intron | 35.0% | |
| !! | TGTTCCATTTTCAAGACTGA+AGG | - | chr5_2:18012042-18012061 | Msa0761020:intron | 35.0% |
| TCAAGATAAAAACTCAGGCC+AGG | - | chr5_2:18011472-18011491 | Msa0761020:intron | 40.0% | |
| CAAGATAAAAACTCAGGCCA+GGG | - | chr5_2:18011473-18011492 | Msa0761020:intron | 40.0% | |
| GTCAATGATCAATTTACCCC+CGG | - | chr5_2:18011776-18011795 | Msa0761020:intron | 40.0% | |
| AAACTCTTATGCAGTGATGG+TGG | - | chr5_2:18011943-18011962 | Msa0761020:intron | 40.0% | |
| TCACTGCTACTCTCAATAAC+TGG | + | chr5_2:18011979-18011998 | None:intergenic | 40.0% | |
| ATTGAGAGTAGCAGTGACAA+TGG | - | chr5_2:18011982-18012001 | Msa0761020:intron | 40.0% | |
| AGTGAAACATTCTGCAAGTG+TGG | - | chr5_2:18012006-18012025 | Msa0761020:intron | 40.0% | |
| TCAAGATAAAAACTCAGGCC+AGG | - | chr5_2:18011472-18011491 | Msa0761020:intron | 40.0% | |
| CAAGATAAAAACTCAGGCCA+GGG | - | chr5_2:18011473-18011492 | Msa0761020:intron | 40.0% | |
| GTCAATGATCAATTTACCCC+CGG | - | chr5_2:18011776-18011795 | Msa0761020:intron | 40.0% | |
| AAACTCTTATGCAGTGATGG+TGG | - | chr5_2:18011943-18011962 | Msa0761020:intron | 40.0% | |
| TCACTGCTACTCTCAATAAC+TGG | + | chr5_2:18011979-18011998 | None:intergenic | 40.0% | |
| ATTGAGAGTAGCAGTGACAA+TGG | - | chr5_2:18011982-18012001 | Msa0761020:intron | 40.0% | |
| AGTGAAACATTCTGCAAGTG+TGG | - | chr5_2:18012006-18012025 | Msa0761020:intron | 40.0% | |
| TTGCTCTTGAGTGAAGCTCT+GGG | + | chr5_2:18011271-18011290 | None:intergenic | 45.0% | |
| AGAGCTTCACTCAAGAGCAA+GGG | - | chr5_2:18011271-18011290 | Msa0761020:CDS | 45.0% | |
| GTGTTGTGCAAGACAGGTAA+AGG | - | chr5_2:18011506-18011525 | Msa0761020:intron | 45.0% | |
| TGTGCAAGACAGGTAAAGGT+TGG | - | chr5_2:18011510-18011529 | Msa0761020:intron | 45.0% | |
| GCAAAATCTTTGCCAGCATC+AGG | + | chr5_2:18011563-18011582 | None:intergenic | 45.0% | |
| ACATTCTGCAAGTGTGGTGA+AGG | - | chr5_2:18012012-18012031 | Msa0761020:intron | 45.0% | |
| CATTCTGCAAGTGTGGTGAA+GGG | - | chr5_2:18012013-18012032 | Msa0761020:intron | 45.0% | |
| AGGGCCTTCAGTCTTGAAAA+TGG | + | chr5_2:18012049-18012068 | None:intergenic | 45.0% | |
| !! | ATTTTCAAGACTGAAGGCCC+TGG | - | chr5_2:18012048-18012067 | Msa0761020:intron | 45.0% |
| TTGCTCTTGAGTGAAGCTCT+GGG | + | chr5_2:18011271-18011290 | None:intergenic | 45.0% | |
| AGAGCTTCACTCAAGAGCAA+GGG | - | chr5_2:18011271-18011290 | Msa0761020:CDS | 45.0% | |
| GTGTTGTGCAAGACAGGTAA+AGG | - | chr5_2:18011506-18011525 | Msa0761020:intron | 45.0% | |
| TGTGCAAGACAGGTAAAGGT+TGG | - | chr5_2:18011510-18011529 | Msa0761020:intron | 45.0% | |
| GCAAAATCTTTGCCAGCATC+AGG | + | chr5_2:18011563-18011582 | None:intergenic | 45.0% | |
| ACATTCTGCAAGTGTGGTGA+AGG | - | chr5_2:18012012-18012031 | Msa0761020:intron | 45.0% | |
| CATTCTGCAAGTGTGGTGAA+GGG | - | chr5_2:18012013-18012032 | Msa0761020:intron | 45.0% | |
| AGGGCCTTCAGTCTTGAAAA+TGG | + | chr5_2:18012049-18012068 | None:intergenic | 45.0% | |
| !! | ATTTTCAAGACTGAAGGCCC+TGG | - | chr5_2:18012048-18012067 | Msa0761020:intron | 45.0% |
| !! | TTAAAATAATTAATAATAAG+AGG | + | chr5_2:18011633-18011652 | None:intergenic | 5.0% |
| !! | TTAAAATAATTAATAATAAG+AGG | + | chr5_2:18011633-18011652 | None:intergenic | 5.0% |
| CTTGCTCTTGAGTGAAGCTC+TGG | + | chr5_2:18011272-18011291 | None:intergenic | 50.0% | |
| CAGAGCTTCACTCAAGAGCA+AGG | - | chr5_2:18011270-18011289 | Msa0761020:CDS | 50.0% | |
| TAAAAACTCAGGCCAGGGCT+TGG | - | chr5_2:18011478-18011497 | Msa0761020:intron | 50.0% | |
| AAAAACTCAGGCCAGGGCTT+GGG | - | chr5_2:18011479-18011498 | Msa0761020:intron | 50.0% | |
| GGCAATGTGTTGTGCAAGAC+AGG | - | chr5_2:18011500-18011519 | Msa0761020:intron | 50.0% | |
| !! | TTATGCAGTGATGGTGGAGC+TGG | - | chr5_2:18011949-18011968 | Msa0761020:intron | 50.0% |
| !! | TATGCAGTGATGGTGGAGCT+GGG | - | chr5_2:18011950-18011969 | Msa0761020:intron | 50.0% |
| CCTTGCCAGAGTTTACACCA+GGG | + | chr5_2:18012068-18012087 | None:intergenic | 50.0% | |
| CCTGGTGTAAACTCTGGCAA+GGG | - | chr5_2:18012066-18012085 | Msa0761020:intron | 50.0% | |
| CTTGCTCTTGAGTGAAGCTC+TGG | + | chr5_2:18011272-18011291 | None:intergenic | 50.0% | |
| CAGAGCTTCACTCAAGAGCA+AGG | - | chr5_2:18011270-18011289 | Msa0761020:CDS | 50.0% | |
| TAAAAACTCAGGCCAGGGCT+TGG | - | chr5_2:18011478-18011497 | Msa0761020:intron | 50.0% | |
| AAAAACTCAGGCCAGGGCTT+GGG | - | chr5_2:18011479-18011498 | Msa0761020:intron | 50.0% | |
| GGCAATGTGTTGTGCAAGAC+AGG | - | chr5_2:18011500-18011519 | Msa0761020:intron | 50.0% | |
| !! | TTATGCAGTGATGGTGGAGC+TGG | - | chr5_2:18011949-18011968 | Msa0761020:intron | 50.0% |
| !! | TATGCAGTGATGGTGGAGCT+GGG | - | chr5_2:18011950-18011969 | Msa0761020:intron | 50.0% |
| CCTTGCCAGAGTTTACACCA+GGG | + | chr5_2:18012068-18012087 | None:intergenic | 50.0% | |
| CCTGGTGTAAACTCTGGCAA+GGG | - | chr5_2:18012066-18012085 | Msa0761020:intron | 50.0% | |
| ACAACACATTGCCCAAGCCC+TGG | + | chr5_2:18011493-18011512 | None:intergenic | 55.0% | |
| TCTGCAAGTGTGGTGAAGGG+TGG | - | chr5_2:18012016-18012035 | Msa0761020:intron | 55.0% | |
| GAAGGCCCTGGTGTAAACTC+TGG | - | chr5_2:18012060-18012079 | Msa0761020:intron | 55.0% | |
| CCCTGGTGTAAACTCTGGCA+AGG | - | chr5_2:18012065-18012084 | Msa0761020:intron | 55.0% | |
| CCCTTGCCAGAGTTTACACC+AGG | + | chr5_2:18012069-18012088 | None:intergenic | 55.0% | |
| ACAACACATTGCCCAAGCCC+TGG | + | chr5_2:18011493-18011512 | None:intergenic | 55.0% | |
| TCTGCAAGTGTGGTGAAGGG+TGG | - | chr5_2:18012016-18012035 | Msa0761020:intron | 55.0% | |
| GAAGGCCCTGGTGTAAACTC+TGG | - | chr5_2:18012060-18012079 | Msa0761020:intron | 55.0% | |
| CCCTGGTGTAAACTCTGGCA+AGG | - | chr5_2:18012065-18012084 | Msa0761020:intron | 55.0% | |
| CCCTTGCCAGAGTTTACACC+AGG | + | chr5_2:18012069-18012088 | None:intergenic | 55.0% | |
| AGGACTGAAGGGCCTGATGC+TGG | - | chr5_2:18011548-18011567 | Msa0761020:intron | 60.0% | |
| AGGACTGAAGGGCCTGATGC+TGG | - | chr5_2:18011548-18011567 | Msa0761020:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 18011261 | 18012134 | 18011261 | ID=Msa0761020;Name=Msa0761020 |
| chr5_2 | mRNA | 18011261 | 18012134 | 18011261 | ID=Msa0761020-mRNA-1;Parent=Msa0761020;Name=Msa0761020-mRNA-1;_AED=0.36;_eAED=0.37;_QI=0|0|0|1|1|1|3|0|120 |
| chr5_2 | exon | 18012103 | 18012134 | 18012103 | ID=Msa0761020-mRNA-1:exon:7849;Parent=Msa0761020-mRNA-1 |
| chr5_2 | exon | 18011783 | 18011935 | 18011783 | ID=Msa0761020-mRNA-1:exon:7848;Parent=Msa0761020-mRNA-1 |
| chr5_2 | exon | 18011261 | 18011438 | 18011261 | ID=Msa0761020-mRNA-1:exon:7847;Parent=Msa0761020-mRNA-1 |
| chr5_2 | CDS | 18012103 | 18012134 | 18012103 | ID=Msa0761020-mRNA-1:cds;Parent=Msa0761020-mRNA-1 |
| chr5_2 | CDS | 18011783 | 18011935 | 18011783 | ID=Msa0761020-mRNA-1:cds;Parent=Msa0761020-mRNA-1 |
| chr5_2 | CDS | 18011261 | 18011438 | 18011261 | ID=Msa0761020-mRNA-1:cds;Parent=Msa0761020-mRNA-1 |
| Gene Sequence |
| Protein sequence |
