AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0720490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0720490 MtrunA17_Chr5g0411241 96.667 120 4 0 1 120 1 120 7.54e-81 233
Msa0720490 MtrunA17_Chr5g0411231 82.500 120 21 0 1 120 1 120 5.81e-68 200
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGGCCTTCAGTCTTGAAAA+TGG 0.356723 5_1:+16523925 None:intergenic
TTATGCAGTGATGGTGGAGC+TGG 0.397441 5_1:-16524022 Msa0720490:intron
CTTGCTCTTGAGTGAAGCTC+TGG 0.411535 5_1:+16524684 None:intergenic
TCACTGCTACTCTCAATAAC+TGG 0.438274 5_1:+16523995 None:intergenic
CCCTTGCCAGAGTTTACACC+AGG 0.459349 5_1:+16523905 None:intergenic
AGGACTGAAGGGCCTGATGC+TGG 0.490624 5_1:-16524418 Msa0720490:CDS
GGCAATGTGTTGTGCAAGAC+AGG 0.514042 5_1:-16524466 Msa0720490:CDS
ACAACACATTGCCCAAGCCC+TGG 0.525921 5_1:+16524476 None:intergenic
CCCTGGTGTAAACTCTGGCA+AGG 0.541663 5_1:-16523906 Msa0720490:CDS
TATGCAGTGATGGTGGAGCT+GGG 0.557903 5_1:-16524021 Msa0720490:intron
ACATTCTGCAAGTGTGGTGA+AGG 0.561546 5_1:-16523959 Msa0720490:CDS
ATTGAGAGTAGCAGTGACAA+TGG 0.563224 5_1:-16523989 Msa0720490:CDS
CAGAGCTTCACTCAAGAGCA+AGG 0.567749 5_1:-16524683 Msa0720490:CDS
TTGCTCTTGAGTGAAGCTCT+GGG 0.573531 5_1:+16524685 None:intergenic
GTTGGACATGTGTTATAACT+AGG 0.581816 5_1:-16524438 Msa0720490:CDS
GTGTTATAACTAGGACTGAA+GGG 0.595455 5_1:-16524429 Msa0720490:CDS
TGTGCAAGACAGGTAAAGGT+TGG 0.598871 5_1:-16524456 Msa0720490:CDS
AAACTCTTATGCAGTGATGG+TGG 0.602169 5_1:-16524028 Msa0720490:intron
GAAGGCCCTGGTGTAAACTC+TGG 0.603912 5_1:-16523911 Msa0720490:CDS
TGTGTTATAACTAGGACTGA+AGG 0.610701 5_1:-16524430 Msa0720490:CDS
CCTGGTGTAAACTCTGGCAA+GGG 0.620143 5_1:-16523905 Msa0720490:CDS
GTGTTGTGCAAGACAGGTAA+AGG 0.626364 5_1:-16524460 Msa0720490:CDS
AGAGCTTCACTCAAGAGCAA+GGG 0.651871 5_1:-16524682 Msa0720490:intron
TCTGCAAGTGTGGTGAAGGG+TGG 0.652623 5_1:-16523955 Msa0720490:CDS
AGTGAAACATTCTGCAAGTG+TGG 0.671634 5_1:-16523965 Msa0720490:CDS
CATTCTGCAAGTGTGGTGAA+GGG 0.681189 5_1:-16523958 Msa0720490:CDS
CCTTGCCAGAGTTTACACCA+GGG 0.773669 5_1:+16523906 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTGTAAAGATGAAATAA+AGG + chr5_1:16523924-16523943 None:intergenic 20.0%
!!! TTTTCTTTTACTCCAAAATA+AGG - chr5_1:16523975-16523994 Msa0720490:CDS 20.0%
!! TAAAATAATAATAAGAGGAG+TGG + chr5_1:16524213-16524232 None:intergenic 20.0%
!! GCAGTTAAAATAATAATAAG+AGG + chr5_1:16524218-16524237 None:intergenic 20.0%
!! AAACTGTAAAGATGAAATAA+AGG + chr5_1:16523924-16523943 None:intergenic 20.0%
!!! TTTTCTTTTACTCCAAAATA+AGG - chr5_1:16523975-16523994 Msa0720490:CDS 20.0%
!! TAAAATAATAATAAGAGGAG+TGG + chr5_1:16524213-16524232 None:intergenic 20.0%
!! GCAGTTAAAATAATAATAAG+AGG + chr5_1:16524218-16524237 None:intergenic 20.0%
! TGTAAAGATGAAATAAAGGA+TGG + chr5_1:16523920-16523939 None:intergenic 25.0%
! AATTATGACAGGTATGAAAA+TGG + chr5_1:16524005-16524024 None:intergenic 25.0%
!! TTTCTAACATTTTCATAACC+GGG + chr5_1:16524378-16524397 None:intergenic 25.0%
!! GTTTCTAACATTTTCATAAC+CGG + chr5_1:16524379-16524398 None:intergenic 25.0%
! TGTAAAGATGAAATAAAGGA+TGG + chr5_1:16523920-16523939 None:intergenic 25.0%
! AATTATGACAGGTATGAAAA+TGG + chr5_1:16524005-16524024 None:intergenic 25.0%
!! TTTCTAACATTTTCATAACC+GGG + chr5_1:16524378-16524397 None:intergenic 25.0%
!! GTTTCTAACATTTTCATAAC+CGG + chr5_1:16524379-16524398 None:intergenic 25.0%
! GCTTATTGGTTAATTATGAC+AGG + chr5_1:16524016-16524035 None:intergenic 30.0%
! TTAACCAATAAGCTTTGTGT+AGG - chr5_1:16524023-16524042 Msa0720490:intron 30.0%
TGAAAATGTCAAATACTGCT+TGG + chr5_1:16524289-16524308 None:intergenic 30.0%
! TTCTAACATTTTCATAACCG+GGG + chr5_1:16524377-16524396 None:intergenic 30.0%
! GCTTATTGGTTAATTATGAC+AGG + chr5_1:16524016-16524035 None:intergenic 30.0%
! TTAACCAATAAGCTTTGTGT+AGG - chr5_1:16524023-16524042 Msa0720490:intron 30.0%
TGAAAATGTCAAATACTGCT+TGG + chr5_1:16524289-16524308 None:intergenic 30.0%
! TTCTAACATTTTCATAACCG+GGG + chr5_1:16524377-16524396 None:intergenic 30.0%
CTTTCCTACACAAAGCTTAT+TGG + chr5_1:16524030-16524049 None:intergenic 35.0%
! CCTGAGTTTTTATCTTGAAC+TGG + chr5_1:16524055-16524074 None:intergenic 35.0%
CCAGTTCAAGATAAAAACTC+AGG - chr5_1:16524052-16524071 Msa0720490:intron 35.0%
GTTGGACATGTGTTATAACT+AGG - chr5_1:16524113-16524132 Msa0720490:intron 35.0%
TGTGTTATAACTAGGACTGA+AGG - chr5_1:16524121-16524140 Msa0720490:intron 35.0%
GTGTTATAACTAGGACTGAA+GGG - chr5_1:16524122-16524141 Msa0720490:intron 35.0%
! GGCAAAGTTTTTGCTAAATG+TGG - chr5_1:16524154-16524173 Msa0720490:intron 35.0%
! TCTAACATTTTCATAACCGG+GGG + chr5_1:16524376-16524395 None:intergenic 35.0%
ATCAAACTCTTATGCAGTGA+TGG - chr5_1:16524520-16524539 Msa0720490:intron 35.0%
!! TGTTCCATTTTCAAGACTGA+AGG - chr5_1:16524622-16524641 Msa0720490:intron 35.0%
CTTTCCTACACAAAGCTTAT+TGG + chr5_1:16524030-16524049 None:intergenic 35.0%
! CCTGAGTTTTTATCTTGAAC+TGG + chr5_1:16524055-16524074 None:intergenic 35.0%
CCAGTTCAAGATAAAAACTC+AGG - chr5_1:16524052-16524071 Msa0720490:intron 35.0%
GTTGGACATGTGTTATAACT+AGG - chr5_1:16524113-16524132 Msa0720490:intron 35.0%
TGTGTTATAACTAGGACTGA+AGG - chr5_1:16524121-16524140 Msa0720490:intron 35.0%
GTGTTATAACTAGGACTGAA+GGG - chr5_1:16524122-16524141 Msa0720490:intron 35.0%
! GGCAAAGTTTTTGCTAAATG+TGG - chr5_1:16524154-16524173 Msa0720490:intron 35.0%
! TCTAACATTTTCATAACCGG+GGG + chr5_1:16524376-16524395 None:intergenic 35.0%
ATCAAACTCTTATGCAGTGA+TGG - chr5_1:16524520-16524539 Msa0720490:intron 35.0%
!! TGTTCCATTTTCAAGACTGA+AGG - chr5_1:16524622-16524641 Msa0720490:intron 35.0%
!!! GAAAATGGAGCGCCTTATTT+TGG + chr5_1:16523990-16524009 None:intergenic 40.0%
TCAAGATAAAAACTCAGGCC+AGG - chr5_1:16524057-16524076 Msa0720490:intron 40.0%
CAAGATAAAAACTCAGGCCA+GGG - chr5_1:16524058-16524077 Msa0720490:intron 40.0%
GTCAATGATCAATTTACCCC+CGG - chr5_1:16524357-16524376 Msa0720490:intron 40.0%
!! TTGACGTCTGAATTGATTCG+TGG - chr5_1:16524419-16524438 Msa0720490:CDS 40.0%
AAACTCTTATGCAGTGATGG+TGG - chr5_1:16524523-16524542 Msa0720490:intron 40.0%
TCACTGCTACTCTCAATAAC+TGG + chr5_1:16524559-16524578 None:intergenic 40.0%
ATTGAGAGTAGCAGTGACAA+TGG - chr5_1:16524562-16524581 Msa0720490:intron 40.0%
AGTGAAACATTCTGCAAGTG+TGG - chr5_1:16524586-16524605 Msa0720490:intron 40.0%
!!! GAAAATGGAGCGCCTTATTT+TGG + chr5_1:16523990-16524009 None:intergenic 40.0%
TCAAGATAAAAACTCAGGCC+AGG - chr5_1:16524057-16524076 Msa0720490:intron 40.0%
CAAGATAAAAACTCAGGCCA+GGG - chr5_1:16524058-16524077 Msa0720490:intron 40.0%
GTCAATGATCAATTTACCCC+CGG - chr5_1:16524357-16524376 Msa0720490:intron 40.0%
!! TTGACGTCTGAATTGATTCG+TGG - chr5_1:16524419-16524438 Msa0720490:CDS 40.0%
AAACTCTTATGCAGTGATGG+TGG - chr5_1:16524523-16524542 Msa0720490:intron 40.0%
TCACTGCTACTCTCAATAAC+TGG + chr5_1:16524559-16524578 None:intergenic 40.0%
ATTGAGAGTAGCAGTGACAA+TGG - chr5_1:16524562-16524581 Msa0720490:intron 40.0%
AGTGAAACATTCTGCAAGTG+TGG - chr5_1:16524586-16524605 Msa0720490:intron 40.0%
TTGCTCTTGAGTGAAGCTCT+GGG + chr5_1:16523869-16523888 None:intergenic 45.0%
AGAGCTTCACTCAAGAGCAA+GGG - chr5_1:16523869-16523888 Msa0720490:CDS 45.0%
GTGTTGTGCAAGACAGGTAA+AGG - chr5_1:16524091-16524110 Msa0720490:intron 45.0%
TGTGCAAGACAGGTAAAGGT+TGG - chr5_1:16524095-16524114 Msa0720490:intron 45.0%
GCAAAAACTTTGCCAGCATC+AGG + chr5_1:16524148-16524167 None:intergenic 45.0%
ACATTCTGCAAGTGTGGTGA+AGG - chr5_1:16524592-16524611 Msa0720490:intron 45.0%
CATTCTGCAAGTGTGGTGAA+GGG - chr5_1:16524593-16524612 Msa0720490:intron 45.0%
AGGGCCTTCAGTCTTGAAAA+TGG + chr5_1:16524629-16524648 None:intergenic 45.0%
!! ATTTTCAAGACTGAAGGCCC+TGG - chr5_1:16524628-16524647 Msa0720490:intron 45.0%
TTGCTCTTGAGTGAAGCTCT+GGG + chr5_1:16523869-16523888 None:intergenic 45.0%
AGAGCTTCACTCAAGAGCAA+GGG - chr5_1:16523869-16523888 Msa0720490:CDS 45.0%
GTGTTGTGCAAGACAGGTAA+AGG - chr5_1:16524091-16524110 Msa0720490:intron 45.0%
TGTGCAAGACAGGTAAAGGT+TGG - chr5_1:16524095-16524114 Msa0720490:intron 45.0%
GCAAAAACTTTGCCAGCATC+AGG + chr5_1:16524148-16524167 None:intergenic 45.0%
ACATTCTGCAAGTGTGGTGA+AGG - chr5_1:16524592-16524611 Msa0720490:intron 45.0%
CATTCTGCAAGTGTGGTGAA+GGG - chr5_1:16524593-16524612 Msa0720490:intron 45.0%
AGGGCCTTCAGTCTTGAAAA+TGG + chr5_1:16524629-16524648 None:intergenic 45.0%
!! ATTTTCAAGACTGAAGGCCC+TGG - chr5_1:16524628-16524647 Msa0720490:intron 45.0%
CTTGCTCTTGAGTGAAGCTC+TGG + chr5_1:16523870-16523889 None:intergenic 50.0%
CAGAGCTTCACTCAAGAGCA+AGG - chr5_1:16523868-16523887 Msa0720490:CDS 50.0%
TAAAAACTCAGGCCAGGGCT+TGG - chr5_1:16524063-16524082 Msa0720490:intron 50.0%
AAAAACTCAGGCCAGGGCTT+GGG - chr5_1:16524064-16524083 Msa0720490:intron 50.0%
GGCAATGTGTTGTGCAAGAC+AGG - chr5_1:16524085-16524104 Msa0720490:intron 50.0%
!! TTATGCAGTGATGGTGGAGC+TGG - chr5_1:16524529-16524548 Msa0720490:intron 50.0%
!! TATGCAGTGATGGTGGAGCT+GGG - chr5_1:16524530-16524549 Msa0720490:intron 50.0%
CCTTGCCAGAGTTTACACCA+GGG + chr5_1:16524648-16524667 None:intergenic 50.0%
CCTGGTGTAAACTCTGGCAA+GGG - chr5_1:16524646-16524665 Msa0720490:intron 50.0%
CTTGCTCTTGAGTGAAGCTC+TGG + chr5_1:16523870-16523889 None:intergenic 50.0%
CAGAGCTTCACTCAAGAGCA+AGG - chr5_1:16523868-16523887 Msa0720490:CDS 50.0%
TAAAAACTCAGGCCAGGGCT+TGG - chr5_1:16524063-16524082 Msa0720490:intron 50.0%
AAAAACTCAGGCCAGGGCTT+GGG - chr5_1:16524064-16524083 Msa0720490:intron 50.0%
GGCAATGTGTTGTGCAAGAC+AGG - chr5_1:16524085-16524104 Msa0720490:intron 50.0%
!! TTATGCAGTGATGGTGGAGC+TGG - chr5_1:16524529-16524548 Msa0720490:intron 50.0%
!! TATGCAGTGATGGTGGAGCT+GGG - chr5_1:16524530-16524549 Msa0720490:intron 50.0%
CCTTGCCAGAGTTTACACCA+GGG + chr5_1:16524648-16524667 None:intergenic 50.0%
CCTGGTGTAAACTCTGGCAA+GGG - chr5_1:16524646-16524665 Msa0720490:intron 50.0%
ACAACACATTGCCCAAGCCC+TGG + chr5_1:16524078-16524097 None:intergenic 55.0%
TCTGCAAGTGTGGTGAAGGG+TGG - chr5_1:16524596-16524615 Msa0720490:intron 55.0%
GAAGGCCCTGGTGTAAACTC+TGG - chr5_1:16524640-16524659 Msa0720490:intron 55.0%
CCCTGGTGTAAACTCTGGCA+AGG - chr5_1:16524645-16524664 Msa0720490:intron 55.0%
CCCTTGCCAGAGTTTACACC+AGG + chr5_1:16524649-16524668 None:intergenic 55.0%
ACAACACATTGCCCAAGCCC+TGG + chr5_1:16524078-16524097 None:intergenic 55.0%
TCTGCAAGTGTGGTGAAGGG+TGG - chr5_1:16524596-16524615 Msa0720490:intron 55.0%
GAAGGCCCTGGTGTAAACTC+TGG - chr5_1:16524640-16524659 Msa0720490:intron 55.0%
CCCTGGTGTAAACTCTGGCA+AGG - chr5_1:16524645-16524664 Msa0720490:intron 55.0%
CCCTTGCCAGAGTTTACACC+AGG + chr5_1:16524649-16524668 None:intergenic 55.0%
AGGACTGAAGGGCCTGATGC+TGG - chr5_1:16524133-16524152 Msa0720490:intron 60.0%
AGGACTGAAGGGCCTGATGC+TGG - chr5_1:16524133-16524152 Msa0720490:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 16523859 16524714 16523859 ID=Msa0720490;Name=Msa0720490
chr5_1 mRNA 16523859 16524714 16523859 ID=Msa0720490-mRNA-1;Parent=Msa0720490;Name=Msa0720490-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|0|0|1|1|1|3|0|120
chr5_1 exon 16524683 16524714 16524683 ID=Msa0720490-mRNA-1:exon:6299;Parent=Msa0720490-mRNA-1
chr5_1 exon 16524376 16524528 16524376 ID=Msa0720490-mRNA-1:exon:6298;Parent=Msa0720490-mRNA-1
chr5_1 exon 16523859 16524036 16523859 ID=Msa0720490-mRNA-1:exon:6297;Parent=Msa0720490-mRNA-1
chr5_1 CDS 16524683 16524714 16524683 ID=Msa0720490-mRNA-1:cds;Parent=Msa0720490-mRNA-1
chr5_1 CDS 16524376 16524528 16524376 ID=Msa0720490-mRNA-1:cds;Parent=Msa0720490-mRNA-1
chr5_1 CDS 16523859 16524036 16523859 ID=Msa0720490-mRNA-1:cds;Parent=Msa0720490-mRNA-1
Gene Sequence

>Msa0720490

ATGGAGTCCCAGAGCTTCACTCAAGAGCAAGGGAAAGTTCCAGTTCAAGATAAAAACTCAGGCCAGGGCTTGGGCAATGTGTTGTGCAAGACAGGTAAAGGTTGGACATGTGTTATAACTAGGACTGAAGGGCCTGATGCTGGCAAAGTTTTTGCTAAATGTGGTGAAAATTGCAACTGCACGCTTGATGGTGGAGCTGGGTCACCAGTTATTGAGAGTAGCAGTGACAATGGCAGTGAAACATTCTGCAAGTGTGGTGAAGGGTGGAGTTGTTCCATTTTCAAGACTGAAGGCCCTGGTGTAAACTCTGGCAAGGGCTTTGCTGAATGCTCTCAACTGTATAACTGTGCTTGTAACTGTTGA

Protein sequence

>Msa0720490

MESQSFTQEQGKVPVQDKNSGQGLGNVLCKTGKGWTCVITRTEGPDAGKVFAKCGENCNCTLDGGAGSPVIESSSDNGSETFCKCGEGWSCSIFKTEGPGVNSGKGFAECSQLYNCACNC*