AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0841670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0841670 MtrunA17_Chr5g0411241 89.908 109 11 0 1 109 12 120 5.97e-67 197
Msa0841670 MtrunA17_Chr5g0411231 76.147 109 26 0 1 109 12 120 7.52e-57 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACTCAGGCCAGGGCTTT+GGG 0.334176 5_4:-18154112 Msa0841670:CDS
AGGGCCTTCAGTCTTGAAAA+TGG 0.356723 5_4:+18153433 None:intergenic
TTATGCAGTGATGGTGGAGC+TGG 0.397441 5_4:-18153530 Msa0841670:intron
TCACTGCTACTCTCAATAAC+TGG 0.438274 5_4:+18153503 None:intergenic
CCCTTGCCAGAGTTTACACC+AGG 0.459349 5_4:+18153413 None:intergenic
AGGACTGAAGGGCCTGATGC+TGG 0.490624 5_4:-18154043 Msa0841670:CDS
GGCAATGTGTTGTGCAAGAC+AGG 0.497539 5_4:-18154091 Msa0841670:CDS
CAACACATTGCCCAAAGCCC+TGG 0.502645 5_4:+18154102 None:intergenic
CCCTGGTGTAAACTCTGGCA+AGG 0.541663 5_4:-18153414 Msa0841670:CDS
TATGCAGTGATGGTGGAGCT+GGG 0.557903 5_4:-18153529 Msa0841670:intron
ACATTCTGCAAGTGTGGTGA+AGG 0.561546 5_4:-18153467 Msa0841670:CDS
ATTGAGAGTAGCAGTGACAA+TGG 0.563224 5_4:-18153497 Msa0841670:CDS
GTTGGACATGTGTTATAACT+AGG 0.581816 5_4:-18154063 Msa0841670:CDS
GTGTTATAACTAGGACTGAA+GGG 0.595455 5_4:-18154054 Msa0841670:CDS
TGTGCAAGACAGGTAAAGGT+TGG 0.598871 5_4:-18154081 Msa0841670:CDS
AAACTCTTATGCAGTGATGG+TGG 0.602169 5_4:-18153536 Msa0841670:intron
GAAGGCCCTGGTGTAAACTC+TGG 0.603912 5_4:-18153419 Msa0841670:CDS
TGTGTTATAACTAGGACTGA+AGG 0.610701 5_4:-18154055 Msa0841670:CDS
CCTGGTGTAAACTCTGGCAA+GGG 0.620143 5_4:-18153413 Msa0841670:CDS
GTGTTGTGCAAGACAGGTAA+AGG 0.626364 5_4:-18154085 Msa0841670:CDS
TCTGCAAGTGTGGTGAAGGG+TGG 0.652623 5_4:-18153463 Msa0841670:CDS
AGTGAAACATTCTGCAAGTG+TGG 0.671634 5_4:-18153473 Msa0841670:CDS
CATTCTGCAAGTGTGGTGAA+GGG 0.681189 5_4:-18153466 Msa0841670:CDS
CCTTGCCAGAGTTTACACCA+GGG 0.773669 5_4:+18153414 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTATTTGTTTGTAATA+TGG - chr5_4:18153661-18153680 Msa0841670:intron 10.0%
!! TTAATTATTTGTTTGTAATA+TGG - chr5_4:18153661-18153680 Msa0841670:intron 10.0%
!! TAATATGAAATTGAGATTTA+AGG + chr5_4:18153784-18153803 None:intergenic 15.0%
!! TAATATGAAATTGAGATTTA+AGG + chr5_4:18153784-18153803 None:intergenic 15.0%
!! ATAATTAATAATAAGAGGAG+TGG + chr5_4:18153537-18153556 None:intergenic 20.0%
!! TATCGTGGAAAAAAAATATA+AGG - chr5_4:18153591-18153610 Msa0841670:intron 20.0%
!! TTATTAGGTAAAAGTTCAAT+AGG + chr5_4:18153731-18153750 None:intergenic 20.0%
!! AATTTATCGTTCAACTTATT+AGG + chr5_4:18153746-18153765 None:intergenic 20.0%
!! TTCTATAAGCATAAAATTAG+TGG + chr5_4:18153856-18153875 None:intergenic 20.0%
!! ATAATTAATAATAAGAGGAG+TGG + chr5_4:18153537-18153556 None:intergenic 20.0%
!! TATCGTGGAAAAAAAATATA+AGG - chr5_4:18153591-18153610 Msa0841670:intron 20.0%
!! TTATTAGGTAAAAGTTCAAT+AGG + chr5_4:18153731-18153750 None:intergenic 20.0%
!! AATTTATCGTTCAACTTATT+AGG + chr5_4:18153746-18153765 None:intergenic 20.0%
!! TTCTATAAGCATAAAATTAG+TGG + chr5_4:18153856-18153875 None:intergenic 20.0%
!! GCAATCATTTTAACAATGGT+TGG + chr5_4:18153688-18153707 None:intergenic 30.0%
!! TGGTGCAATCATTTTAACAA+TGG + chr5_4:18153692-18153711 None:intergenic 30.0%
!! GCAATCATTTTAACAATGGT+TGG + chr5_4:18153688-18153707 None:intergenic 30.0%
!! TGGTGCAATCATTTTAACAA+TGG + chr5_4:18153692-18153711 None:intergenic 30.0%
! CTGAGTTTTTATCTTGAGCT+GGG + chr5_4:18153377-18153396 None:intergenic 35.0%
GTTGGACATGTGTTATAACT+AGG - chr5_4:18153437-18153456 Msa0841670:CDS 35.0%
TGTGTTATAACTAGGACTGA+AGG - chr5_4:18153445-18153464 Msa0841670:CDS 35.0%
GTGTTATAACTAGGACTGAA+GGG - chr5_4:18153446-18153465 Msa0841670:CDS 35.0%
! GGCAAAGTTTTTGCTAAATG+TGG - chr5_4:18153478-18153497 Msa0841670:CDS 35.0%
ATTTGACAAGAAAGCTATCG+TGG - chr5_4:18153576-18153595 Msa0841670:intron 35.0%
TAGGTAAGATCTTCATGTCA+TGG + chr5_4:18153712-18153731 None:intergenic 35.0%
ATCAAACTCTTATGCAGTGA+TGG - chr5_4:18153961-18153980 Msa0841670:intron 35.0%
!! TGTTCCATTTTCAAGACTGA+AGG - chr5_4:18154063-18154082 Msa0841670:CDS 35.0%
! CTGAGTTTTTATCTTGAGCT+GGG + chr5_4:18153377-18153396 None:intergenic 35.0%
GTTGGACATGTGTTATAACT+AGG - chr5_4:18153437-18153456 Msa0841670:CDS 35.0%
TGTGTTATAACTAGGACTGA+AGG - chr5_4:18153445-18153464 Msa0841670:CDS 35.0%
GTGTTATAACTAGGACTGAA+GGG - chr5_4:18153446-18153465 Msa0841670:CDS 35.0%
! GGCAAAGTTTTTGCTAAATG+TGG - chr5_4:18153478-18153497 Msa0841670:CDS 35.0%
ATTTGACAAGAAAGCTATCG+TGG - chr5_4:18153576-18153595 Msa0841670:intron 35.0%
TAGGTAAGATCTTCATGTCA+TGG + chr5_4:18153712-18153731 None:intergenic 35.0%
ATCAAACTCTTATGCAGTGA+TGG - chr5_4:18153961-18153980 Msa0841670:intron 35.0%
!! TGTTCCATTTTCAAGACTGA+AGG - chr5_4:18154063-18154082 Msa0841670:CDS 35.0%
! CCTGAGTTTTTATCTTGAGC+TGG + chr5_4:18153378-18153397 None:intergenic 40.0%
CCAGCTCAAGATAAAAACTC+AGG - chr5_4:18153375-18153394 Msa0841670:CDS 40.0%
TCAAGATAAAAACTCAGGCC+AGG - chr5_4:18153380-18153399 Msa0841670:CDS 40.0%
CAAGATAAAAACTCAGGCCA+GGG - chr5_4:18153381-18153400 Msa0841670:CDS 40.0%
AAACTCTTATGCAGTGATGG+TGG - chr5_4:18153964-18153983 Msa0841670:intron 40.0%
TCACTGCTACTCTCAATAAC+TGG + chr5_4:18154000-18154019 None:intergenic 40.0%
ATTGAGAGTAGCAGTGACAA+TGG - chr5_4:18154003-18154022 Msa0841670:CDS 40.0%
AGTGAAACATTCTGCAAGTG+TGG - chr5_4:18154027-18154046 Msa0841670:CDS 40.0%
! CCTGAGTTTTTATCTTGAGC+TGG + chr5_4:18153378-18153397 None:intergenic 40.0%
CCAGCTCAAGATAAAAACTC+AGG - chr5_4:18153375-18153394 Msa0841670:CDS 40.0%
TCAAGATAAAAACTCAGGCC+AGG - chr5_4:18153380-18153399 Msa0841670:CDS 40.0%
CAAGATAAAAACTCAGGCCA+GGG - chr5_4:18153381-18153400 Msa0841670:CDS 40.0%
AAACTCTTATGCAGTGATGG+TGG - chr5_4:18153964-18153983 Msa0841670:intron 40.0%
TCACTGCTACTCTCAATAAC+TGG + chr5_4:18154000-18154019 None:intergenic 40.0%
ATTGAGAGTAGCAGTGACAA+TGG - chr5_4:18154003-18154022 Msa0841670:CDS 40.0%
AGTGAAACATTCTGCAAGTG+TGG - chr5_4:18154027-18154046 Msa0841670:CDS 40.0%
GTGTTGTGCAAGACAGGTAA+AGG - chr5_4:18153415-18153434 Msa0841670:CDS 45.0%
TGTGCAAGACAGGTAAAGGT+TGG - chr5_4:18153419-18153438 Msa0841670:CDS 45.0%
GCAAAAACTTTGCCAGCATC+AGG + chr5_4:18153472-18153491 None:intergenic 45.0%
ACATTCTGCAAGTGTGGTGA+AGG - chr5_4:18154033-18154052 Msa0841670:CDS 45.0%
CATTCTGCAAGTGTGGTGAA+GGG - chr5_4:18154034-18154053 Msa0841670:CDS 45.0%
AGGGCCTTCAGTCTTGAAAA+TGG + chr5_4:18154070-18154089 None:intergenic 45.0%
!! ATTTTCAAGACTGAAGGCCC+TGG - chr5_4:18154069-18154088 Msa0841670:CDS 45.0%
GTGTTGTGCAAGACAGGTAA+AGG - chr5_4:18153415-18153434 Msa0841670:CDS 45.0%
TGTGCAAGACAGGTAAAGGT+TGG - chr5_4:18153419-18153438 Msa0841670:CDS 45.0%
GCAAAAACTTTGCCAGCATC+AGG + chr5_4:18153472-18153491 None:intergenic 45.0%
ACATTCTGCAAGTGTGGTGA+AGG - chr5_4:18154033-18154052 Msa0841670:CDS 45.0%
CATTCTGCAAGTGTGGTGAA+GGG - chr5_4:18154034-18154053 Msa0841670:CDS 45.0%
AGGGCCTTCAGTCTTGAAAA+TGG + chr5_4:18154070-18154089 None:intergenic 45.0%
!! ATTTTCAAGACTGAAGGCCC+TGG - chr5_4:18154069-18154088 Msa0841670:CDS 45.0%
!! TTAAAATAATTAATAATAAG+AGG + chr5_4:18153542-18153561 None:intergenic 5.0%
!! TTAAAATAATTAATAATAAG+AGG + chr5_4:18153542-18153561 None:intergenic 5.0%
AAAAACTCAGGCCAGGGCTT+TGG - chr5_4:18153387-18153406 Msa0841670:CDS 50.0%
AAAACTCAGGCCAGGGCTTT+GGG - chr5_4:18153388-18153407 Msa0841670:CDS 50.0%
GGCAATGTGTTGTGCAAGAC+AGG - chr5_4:18153409-18153428 Msa0841670:CDS 50.0%
!! TTATGCAGTGATGGTGGAGC+TGG - chr5_4:18153970-18153989 Msa0841670:intron 50.0%
!! TATGCAGTGATGGTGGAGCT+GGG - chr5_4:18153971-18153990 Msa0841670:intron 50.0%
CCTTGCCAGAGTTTACACCA+GGG + chr5_4:18154089-18154108 None:intergenic 50.0%
CCTGGTGTAAACTCTGGCAA+GGG - chr5_4:18154087-18154106 Msa0841670:CDS 50.0%
AAAAACTCAGGCCAGGGCTT+TGG - chr5_4:18153387-18153406 Msa0841670:CDS 50.0%
AAAACTCAGGCCAGGGCTTT+GGG - chr5_4:18153388-18153407 Msa0841670:CDS 50.0%
GGCAATGTGTTGTGCAAGAC+AGG - chr5_4:18153409-18153428 Msa0841670:CDS 50.0%
!! TTATGCAGTGATGGTGGAGC+TGG - chr5_4:18153970-18153989 Msa0841670:intron 50.0%
!! TATGCAGTGATGGTGGAGCT+GGG - chr5_4:18153971-18153990 Msa0841670:intron 50.0%
CCTTGCCAGAGTTTACACCA+GGG + chr5_4:18154089-18154108 None:intergenic 50.0%
CCTGGTGTAAACTCTGGCAA+GGG - chr5_4:18154087-18154106 Msa0841670:CDS 50.0%
CAACACATTGCCCAAAGCCC+TGG + chr5_4:18153401-18153420 None:intergenic 55.0%
TCTGCAAGTGTGGTGAAGGG+TGG - chr5_4:18154037-18154056 Msa0841670:CDS 55.0%
GAAGGCCCTGGTGTAAACTC+TGG - chr5_4:18154081-18154100 Msa0841670:CDS 55.0%
CCCTGGTGTAAACTCTGGCA+AGG - chr5_4:18154086-18154105 Msa0841670:CDS 55.0%
CCCTTGCCAGAGTTTACACC+AGG + chr5_4:18154090-18154109 None:intergenic 55.0%
CAACACATTGCCCAAAGCCC+TGG + chr5_4:18153401-18153420 None:intergenic 55.0%
TCTGCAAGTGTGGTGAAGGG+TGG - chr5_4:18154037-18154056 Msa0841670:CDS 55.0%
GAAGGCCCTGGTGTAAACTC+TGG - chr5_4:18154081-18154100 Msa0841670:CDS 55.0%
CCCTGGTGTAAACTCTGGCA+AGG - chr5_4:18154086-18154105 Msa0841670:CDS 55.0%
CCCTTGCCAGAGTTTACACC+AGG + chr5_4:18154090-18154109 None:intergenic 55.0%
AGGACTGAAGGGCCTGATGC+TGG - chr5_4:18153457-18153476 Msa0841670:CDS 60.0%
AGGACTGAAGGGCCTGATGC+TGG - chr5_4:18153457-18153476 Msa0841670:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 18153370 18154152 18153370 ID=Msa0841670;Name=Msa0841670
chr5_4 mRNA 18153370 18154152 18153370 ID=Msa0841670-mRNA-1;Parent=Msa0841670;Name=Msa0841670-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|0|0|1|1|0.5|2|0|109
chr5_4 exon 18154001 18154152 18154001 ID=Msa0841670-mRNA-1:exon:7641;Parent=Msa0841670-mRNA-1
chr5_4 exon 18153370 18153544 18153370 ID=Msa0841670-mRNA-1:exon:7640;Parent=Msa0841670-mRNA-1
chr5_4 CDS 18154001 18154152 18154001 ID=Msa0841670-mRNA-1:cds;Parent=Msa0841670-mRNA-1
chr5_4 CDS 18153370 18153544 18153370 ID=Msa0841670-mRNA-1:cds;Parent=Msa0841670-mRNA-1
Gene Sequence

>Msa0841670

AAGTCCCAGCTCAAGATAAAAACTCAGGCCAGGGCTTTGGGCAATGTGTTGTGCAAGACAGGTAAAGGTTGGACATGTGTTATAACTAGGACTGAAGGGCCTGATGCTGGCAAAGTTTTTGCTAAATGTGGTGAAAATTGCAACTGCACGCTTGATGGTGGAGCTGGGTCACCAGTTATTGAGAGTAGCAGTGACAATGGCAGTGAAACATTCTGCAAGTGTGGTGAAGGGTGGAGTTGTTCCATTTTCAAGACTGAAGGCCCTGGTGTAAACTCTGGCAAGGGCTTTGCTGAATGCACTCAACAGTATAACTGTGCTTGTAACTGT

Protein sequence

>Msa0841670

KSQLKIKTQARALGNVLCKTGKGWTCVITRTEGPDAGKVFAKCGENCNCTLDGGAGSPVIESSSDNGSETFCKCGEGWSCSIFKTEGPGVNSGKGFAECTQQYNCACNC