AlfalfaGEDB | Msa0765420

M. sativa cultivar zhongmu-4 / Msa0765420

KEGG: -
GO: -
PFAM: PF03134.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0765420	sp\|Q9S784\|HA22C_ARATH	61.333	150	47	3	2	147	4	146	2.67e-53	186

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0765420	G7KCI6	45.161	496	126	16	123	533	1	435	6.30e-101	331
Msa0765420	G7KCI6	77.419	341	57	5	524	855	1	330	1.15e-164	496

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0136650	Msa0765420	0.839584	1.452107e-57	-8.615850e-47
Msa0222560	Msa0765420	0.807279	5.461474e-50	-8.615850e-47
Msa0372680	Msa0765420	0.935526	7.388528e-97	-8.615850e-47
Msa0380620	Msa0765420	0.843552	1.305968e-58	-8.615850e-47
Msa0390970	Msa0765420	0.805662	1.197825e-49	-8.615850e-47
Msa0430260	Msa0765420	0.852619	4.100162e-61	-8.615850e-47
Msa0500380	Msa0765420	0.828787	7.402652e-55	-8.615850e-47
Msa0626450	Msa0765420	0.807803	4.229056e-50	-8.615850e-47
Msa0626490	Msa0765420	0.807300	5.408032e-50	-8.615850e-47
Msa0664600	Msa0765420	0.806950	6.412635e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0765420	MtrunA17_Chr5g0416561	99.301	143	1	0	1	143	58	200	3.63e-93	293
Msa0765420	MtrunA17_Chr5g0416561	97.500	40	1	0	523	562	179	218	2.72e-18	85.1
Msa0765420	MtrunA17_Chr5g0416551	71.359	206	14	2	204	364	1	206	3.94e-92	289
Msa0765420	MtrunA17_Chr5g0416551	67.164	201	59	4	595	790	8	206	8.26e-81	259
Msa0765420	MtrunA17_Chr4g0060581	67.883	137	34	2	8	143	11	138	2.45e-57	194
Msa0765420	MtrunA17_Chr4g0060581	48.387	62	31	1	515	575	108	169	6.11e-11	62.4
Msa0765420	MtrunA17_Chr5g0443431	57.143	98	42	0	8	105	3	100	7.07e-33	124
Msa0765420	MtrunA17_Chr3g0097141	56.122	98	43	0	8	105	3	100	3.17e-32	122
Msa0765420	MtrunA17_Chr8g0386221	47.619	105	52	1	9	110	4	108	1.91e-28	110
Msa0765420	MtrunA17_Chr3g0132031	50.526	95	47	0	9	103	4	98	3.61e-25	102

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0765420	AT1G69700.1	61.333	150	47	3	2	147	4	146	2.71e-54	186
Msa0765420	AT1G74520.1	60.417	144	47	2	1	143	1	135	3.92e-53	183
Msa0765420	AT1G74520.2	60.417	144	47	2	1	143	1	135	3.92e-53	183
Msa0765420	AT5G62490.1	51.773	141	54	2	1	141	1	127	1.62e-42	152
Msa0765420	AT2G42820.1	57.447	94	40	0	12	105	7	100	2.33e-33	126
Msa0765420	AT5G50720.1	56.471	85	37	0	19	103	14	98	6.41e-25	100
Msa0765420	AT4G24960.1	51.190	84	41	0	20	103	15	98	2.55e-23	97.1
Msa0765420	AT4G24960.3	51.282	78	38	0	26	103	2	79	5.46e-22	93.2
Msa0765420	AT4G24960.2	47.761	67	35	0	37	103	1	67	1.84e-16	76.3

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Find 213 sgRNAs with CRISPR-Local

Find 822 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAATATTCTTGGTCTCTA+TGG	0.262576	5_2:+26341760	None:intergenic
AACACACTTCTATACTAGCA+TGG	0.619651	5_2:+26334767	None:intergenic
AACAGGGGAATCTGCTTTGG+TGG	0.498446	5_2:+26334121	None:intergenic
AACCGACTCTATTCACGCAA+TGG	0.580483	5_2:+26337176	None:intergenic
AACCGGGAGCGGGAACGCAG+TGG	0.615146	5_2:-26330045	Msa0765420:CDS
AACGATTCTGATTGCTGGTC+TGG	0.353943	5_2:+26329551	None:intergenic
AACGATTCTGGTTGCTGGTC+CGG	0.347000	5_2:+26329641	None:intergenic
AACGCAGTGGGTACTTGAAA+AGG	0.514974	5_2:-26330032	Msa0765420:CDS
AACTTACACTTCAAGAACTT+TGG	0.394572	5_2:+26341670	None:intergenic
AACTTACTTTGTGAGACAAA+TGG	0.472121	5_2:-26334592	Msa0765420:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAAAAATTCTGCCAAACA+TGG	-	chr5_2:26329445-26329464	Msa0765420:CDS	30.0%
CAAAAAATTCTGCCAAACAT+GGG	-	chr5_2:26329446-26329465	Msa0765420:CDS	30.0%
AACAATATTCTTGGTCTCTA+TGG	+	chr5_2:26329763-26329782	None:intergenic	30.0%
TTGGTCATCAACAATATTCT+TGG	+	chr5_2:26329772-26329791	None:intergenic	30.0%
AACTTACACTTCAAGAACTT+TGG	+	chr5_2:26329853-26329872	None:intergenic	30.0%
GGTTTATGGAAAAGTTAGAA+AGG	-	chr5_2:26329987-26330006	Msa0765420:CDS	30.0%
TTGCATATTATCTGCATTCA+GGG	+	chr5_2:26330074-26330093	None:intergenic	30.0%
TTTGCATATTATCTGCATTC+AGG	+	chr5_2:26330075-26330094	None:intergenic	30.0%
ACTAACAAAGTCTACATTCA+GGG	-	chr5_2:26330195-26330214	Msa0765420:CDS	30.0%
TTTGCAATTTATCTGCATTC+AGG	-	chr5_2:26330223-26330242	Msa0765420:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	26329337	26342205	26329337	ID=Msa0765420;Name=Msa0765420
chr5_2	mRNA	26329337	26342205	26329337	ID=Msa0765420-mRNA-1;Parent=Msa0765420;Name=Msa0765420-mRNA-1;_AED=0.26;_eAED=0.34;_QI=127\|0.28\|0.2\|1\|0.21\|0.2\|15\|0\|897
chr5_2	exon	26329337	26330249	26329337	ID=Msa0765420-mRNA-1:exon:10468;Parent=Msa0765420-mRNA-1
chr5_2	exon	26330267	26330364	26330267	ID=Msa0765420-mRNA-1:exon:10467;Parent=Msa0765420-mRNA-1
chr5_2	exon	26332469	26332594	26332469	ID=Msa0765420-mRNA-1:exon:10466;Parent=Msa0765420-mRNA-1
chr5_2	exon	26332625	26332632	26332625	ID=Msa0765420-mRNA-1:exon:10465;Parent=Msa0765420-mRNA-1
chr5_2	exon	26333981	26334198	26333981	ID=Msa0765420-mRNA-1:exon:10464;Parent=Msa0765420-mRNA-1
chr5_2	exon	26334223	26334417	26334223	ID=Msa0765420-mRNA-1:exon:10463;Parent=Msa0765420-mRNA-1
chr5_2	exon	26334439	26334685	26334439	ID=Msa0765420-mRNA-1:exon:10462;Parent=Msa0765420-mRNA-1
chr5_2	exon	26334782	26334982	26334782	ID=Msa0765420-mRNA-1:exon:10461;Parent=Msa0765420-mRNA-1
chr5_2	exon	26335022	26335122	26335022	ID=Msa0765420-mRNA-1:exon:10460;Parent=Msa0765420-mRNA-1
chr5_2	exon	26337115	26337263	26337115	ID=Msa0765420-mRNA-1:exon:10459;Parent=Msa0765420-mRNA-1
chr5_2	exon	26338745	26338751	26338745	ID=Msa0765420-mRNA-1:exon:10458;Parent=Msa0765420-mRNA-1
chr5_2	exon	26339175	26339393	26339175	ID=Msa0765420-mRNA-1:exon:10457;Parent=Msa0765420-mRNA-1
chr5_2	exon	26341677	26341799	26341677	ID=Msa0765420-mRNA-1:exon:10456;Parent=Msa0765420-mRNA-1
chr5_2	exon	26341890	26341916	26341890	ID=Msa0765420-mRNA-1:exon:10455;Parent=Msa0765420-mRNA-1
chr5_2	exon	26342017	26342205	26342017	ID=Msa0765420-mRNA-1:exon:10454;Parent=Msa0765420-mRNA-1
chr5_2	five_prime_UTR	26342079	26342205	26342079	ID=Msa0765420-mRNA-1:five_prime_utr;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26342017	26342078	26342017	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26341890	26341916	26341890	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26341677	26341799	26341677	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26339175	26339393	26339175	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26338745	26338751	26338745	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26337115	26337263	26337115	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26335022	26335122	26335022	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26334782	26334982	26334782	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26334439	26334685	26334439	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26334223	26334417	26334223	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26333981	26334198	26333981	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26332625	26332632	26332625	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26332469	26332594	26332469	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26330267	26330364	26330267	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1
chr5_2	CDS	26329337	26330249	26329337	ID=Msa0765420-mRNA-1:cds;Parent=Msa0765420-mRNA-1

Gene Sequence

>Msa0765420

ATGGGTGCTTCTGGCAACAATTTGCTTCAAGTTCTCGCCAAAAACTTTGATGTTCTTGCTCTGCCTTTGGTAACCCTTGTTTATCCTTTATATGCTTCAATCAAGGCCATAGAGACCAAGAATATTGTTGATGACCAACAATGGCTCACTTATTGGGTTCTTTACTCTTTGATCACACTCTTCGAGCTCACATTTGCCAAAGTTCTTGAAGTGCTTCCAATTTGGCCTTATGCAAAGTTGATACTGAGTTGTTGGTTGGTTCTTCCTCACTTTAATGGAGCAGCACATGTTTATAAACGCTATCTTAGACCATTCTACATGAACCCACAATTACCACAAATGCCGCTAATGCCAGGAACTTCGCAAATGTGGTATGTTCCACGGAAGAACATATTCAGTAAGCAAGATGATGTTCTTAGTGCTGCAGAGAGTGTGCAAGACATCACATGGTCACCCTATGAAGGATGGAATCAGAAACTACCACCTCCGTTGCAGTTGGATGTCCATTGCGTGAATAGAGTCGGTTCAATCTTTTGCAACAATCATGTGGTCCATCATAATCCACATCTTGTTGCAAAACAATTTGGCATCAAGTATACCAATGAATATATGGCAAGAGATTTCTACACAAATGCTTCTTCAAAGAGTAAAGATTTTCCTGAATATGAGTCTTGGGTAAGAGGGGTAAGCTTGACCGCGCCACCGGAGTGTTGGGTCGAGAAAATGAGAGCTAAACCGCTGAGTGCAGAGACCTACGACATGCTGATAAGTTCTTTGTGTAAACCGGGGGCAATGTGGGTATCTGAAAAGGAAAATGATACACCGAAGTTTTTACGCTTTAGGGATCTTGAATTAAGAGCTAAGGCGTGGCATTGTTTTTACCATGCTAGACAACTTATTGCTGATAGCATTACTAATATCATAAATGAAGATTTATGGTTAGGGCATTCTTCTATTATTAACTTACTTTGTGAGACAAATGGAGTATGCGACGAGGAATATGGTGACTGCTTTATTAGTCCTGAAGTCATCAACCAATCTTGGTACGATAGTGAGTGGAGGATAGAGGTCAGTTGTTCAAGGCCAATACCAGACGTGCAACCAGAATCGCCCCTATCAGACGTGCGAACAGAATCTTTTAAGTTTCAACAGTTTCAACCTCAACCGACCCAACAGGCTCCTCCTCGACAAAATGTCCAACTTTTCACGATGGTGGATGAGAGCACGAGAGAACATGAGTTTCGGATGTTGCAGTTATGTGATATGCAGACTCGACATGGGTCATCAGGACAACTCCCAAATTGGATGCATAATAGTCATGAATGGATGGGAAACAAGAAGAGGATTTCAAGAGAGCAATTTTTCGATGAAAGATATGGAATCCCTCCACCAAAGCAGATTCCCCTGTTGACTTATTTTCCTCCTCCTTGTCCCCAACCACCAGAACAAGACCCGCAACCGCCACATCAAGAGGGAGATCAAACTAGTGATTCAGGAACTGGTTTAGGAGCTGTCCAAGAGTCGAATTTCAGTTACAGCAGTTTCGCAGCAGCACATGAATTTCCACAAATGTGGTATGTTCCACGGAAGAACATATTCAGCAAGCAAGATGATGTTCTTAGTGCTGCAGAGAGATACATGCAAGAAAATGGAACAGAAGCATTTGAAAGACTCATTAGCAAGACTCGTCTTTCTCGTGTTTCACTTTCATCGAAAAAGAATTACAAAACCGGAATTGGGAGAACTTTCACTCATGCATCAAGGATGCCAATAAAACTTGGCAATGCTTCTTCAACTGTTCCTGAATTTGAGTCTATGGTAAGAGGGGTGAATTTGAAACTTTCTGGGGATGTTATTAATTCTATGTTAGGTTTGACAGCTCCACCGGAGTGTTGGGTCGAGAAAATGAGAGCTGAACAGCTGACTGCAGAGACTTATAACATGCTGTTAAATGCTTTATGTAAACCGGGAGCGGGAACGCAGTGGGTACTTGAAAAGGAAAATAACAAACCGAAGTTTTTACGCCTCAAGGATTTGAACATGATAGCTCAGGCATGGAGTTGTTTTTATCATGCTAGTATAGAAGTGTGTTCGACAAACTATCCGAAACAATTGCTAGTGAAAAATGCGTTTGTTGTGTATGCTTTGTTGAAGGGGAAACCGATAAATTTAGGACGATTGATTGCTGATAGCATTGGTTATATTTCGAATAAAGATTTGTGGTTGGGGCATTCTTCTATCATTCACTTACTTTGTGTGACAAAAGGAGTAAGTGAGGAACCAAGTAACTGCATTGTTCGTCCTGCAGTCATTGACAAATCTTGGTTCGATAGTGAGGTGATAAAAGATGAGAGTGTAAGGCCCCAACCGGACCAGCAACCAGAATCGTTTGATTTTTTTGAAGAAGCAGATACGTATGAGTTTCAACCTCAACCAACCCAACCGGCTCCCCATCAACCAGACCAGCAATCAGAATCGTTTGATTTTACACCTTATCGGTTTAAGTTTCAACCTCAACCGACTCCCTCTCAACAAAATGTCCAACTTTTCACGATGGTGGATGAGAGCACGAGAGAACATGAGTTTCGGATGTTGCAGTTATGTGATATGCAGATTCGACATGGTCATCAGGACAACTACCAAATTGGATGCATAATAGTCATGGATGGGGTACCTGCAAAATTACCACAATAG

Protein sequence

>Msa0765420

MGASGNNLLQVLAKNFDVLALPLVTLVYPLYASIKAIETKNIVDDQQWLTYWVLYSLITLFELTFAKVLEVLPIWPYAKLILSCWLVLPHFNGAAHVYKRYLRPFYMNPQLPQMPLMPGTSQMWYVPRKNIFSKQDDVLSAAESVQDITWSPYEGWNQKLPPPLQLDVHCVNRVGSIFCNNHVVHHNPHLVAKQFGIKYTNEYMARDFYTNASSKSKDFPEYESWVRGVSLTAPPECWVEKMRAKPLSAETYDMLISSLCKPGAMWVSEKENDTPKFLRFRDLELRAKAWHCFYHARQLIADSITNIINEDLWLGHSSIINLLCETNGVCDEEYGDCFISPEVINQSWYDSEWRIEVSCSRPIPDVQPESPLSDVRTESFKFQQFQPQPTQQAPPRQNVQLFTMVDESTREHEFRMLQLCDMQTRHGSSGQLPNWMHNSHEWMGNKKRISREQFFDERYGIPPPKQIPLLTYFPPPCPQPPEQDPQPPHQEGDQTSDSGTGLGAVQESNFSYSSFAAAHEFPQMWYVPRKNIFSKQDDVLSAAERYMQENGTEAFERLISKTRLSRVSLSSKKNYKTGIGRTFTHASRMPIKLGNASSTVPEFESMVRGVNLKLSGDVINSMLGLTAPPECWVEKMRAEQLTAETYNMLLNALCKPGAGTQWVLEKENNKPKFLRLKDLNMIAQAWSCFYHASIEVCSTNYPKQLLVKNAFVVYALLKGKPINLGRLIADSIGYISNKDLWLGHSSIIHLLCVTKGVSEEPSNCIVRPAVIDKSWFDSEVIKDESVRPQPDQQPESFDFFEEADTYEFQPQPTQPAPHQPDQQSESFDFTPYRFKFQPQPTPSQQNVQLFTMVDESTREHEFRMLQLCDMQIRHGHQDNYQIGCIIVMDGVPAKLPQ*