AlfalfaGEDB | Msa0767410

M. sativa cultivar zhongmu-4 / Msa0767410

KEGG: K07466
GO: -
PFAM: PF02721.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0767410	A0A072VHU7	88.462	130	15	0	47	176	1	130	2.28e-75	238

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0062170	Msa0767410	0.818939	1.508509e-52	-8.615850e-47
Msa0234270	Msa0767410	0.949332	1.575143e-107	-8.615850e-47
Msa0234320	Msa0767410	0.977775	1.846569e-144	-8.615850e-47
Msa0483040	Msa0767410	0.850465	1.669142e-60	-8.615850e-47
Msa0715520	Msa0767410	0.925565	1.545172e-90	-8.615850e-47
Msa0767410	Msa0934320	0.895268	1.126612e-75	-8.615850e-47
Msa0767410	Msa1350680	0.914851	1.179453e-84	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0767410	MtrunA17_Chr1g0206481	45.342	161	87	1	7	167	2	161	1.30e-45	152
Msa0767410	MtrunA17_Chr5g0423441	33.540	161	106	1	7	167	2	161	2.68e-25	98.2
Msa0767410	MtrunA17_Chr5g0411141	33.540	161	106	1	7	167	3	162	5.47e-25	98.6
Msa0767410	MtrunA17_Chr2g0278111	33.540	161	106	1	7	167	2	161	5.68e-25	98.6
Msa0767410	MtrunA17_Chr2g0280031	34.228	149	88	2	28	167	4	151	2.68e-21	87.8
Msa0767410	MtrunA17_Chr5g0421841	30.435	161	110	2	7	167	2	160	1.49e-19	82.0
Msa0767410	MtrunA17_Chr5g0421741	33.846	130	85	1	7	136	2	130	7.96e-19	79.3
Msa0767410	MtrunA17_Chr1g0148711	33.846	130	85	1	7	136	2	130	7.96e-19	79.3
Msa0767410	MtrunA17_Chr7g0259081	29.197	137	95	2	28	163	23	158	1.83e-13	65.1
Msa0767410	MtrunA17_Chr3g0093401	28.671	143	100	2	7	148	2	143	2.62e-12	63.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0767410	AT2G05642.1	26.531	98	72	0	24	121	19	116	8.51e-11	60.5

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Find 79 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGCTCTGCAATTAACG+CGG	0.726871	5_2:-30475823	None:intergenic
AAACAAACCTTTGGATGCCA+TGG	0.474075	5_2:-30476940	None:intergenic
AAAGATTGATAACCCTACCC+TGG	0.625407	5_2:-30477108	None:intergenic
AAAGCCCCTTACCGCGGCGG+AGG	0.500473	5_2:-30475706	None:intergenic
AACAAATCCATGGCATCCAA+AGG	0.609245	5_2:+30476933	Msa0767410:exon
AAGATTCAGTTTGTACTTAT+GGG	0.314832	5_2:-30478335	None:intergenic
AAGCTTCTCAATAACATAAA+TGG	0.261519	5_2:-30478275	None:intergenic
AAGGATTGACTTCCAGATCC+AGG	0.487161	5_2:+30477090	Msa0767410:CDS
AAGTTTGCAGACATTGTTGG+TGG	0.561560	5_2:+30477067	Msa0767410:CDS
AATCATGGCCTGCCACAAGG+AGG	0.724370	5_2:+30478438	Msa0767410:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATGAAATTAATTGGACCAA+TGG	-	chr5_2:30475654-30475673	None:intergenic	30.0%
GGTCCAATTAATTTCATGAA+AGG	+	chr5_2:30475656-30475675	Msa0767410:five_prime_UTR	30.0%
AGAAGAAATACAAAGTATGG+TGG	-	chr5_2:30475737-30475756	None:intergenic	30.0%
AATCTTCATCTCTTGAAAGT+AGG	-	chr5_2:30475784-30475803	None:intergenic	30.0%
TCAAGAGATGAAGATTTGTA+TGG	+	chr5_2:30475788-30475807	Msa0767410:intron	30.0%
AACAAATACTGGAAAGTAGA+AGG	-	chr5_2:30475889-30475908	None:intergenic	30.0%
AAGAGTTGGAAGAACAATAA+TGG	+	chr5_2:30476064-30476083	Msa0767410:intron	30.0%
AAAATCCTAAAAGCAAGGAA+AGG	-	chr5_2:30476456-30476475	None:intergenic	30.0%
ATATGCATAGAAACAAATGC+AGG	+	chr5_2:30476547-30476566	Msa0767410:intron	30.0%
TGTTCAAGCACTATAAATAC+AGG	+	chr5_2:30476840-30476859	Msa0767410:five_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	30475477	30478872	30475477	ID=Msa0767410;Name=Msa0767410
chr5_2	mRNA	30475477	30478872	30475477	ID=Msa0767410-mRNA-1;Parent=Msa0767410;Name=Msa0767410-mRNA-1;_AED=0.26;_eAED=0.26;_QI=528\|0.85\|0.87\|1\|0.57\|0.5\|8\|83\|182
chr5_2	exon	30475477	30475716	30475477	ID=Msa0767410-mRNA-1:exon:11476;Parent=Msa0767410-mRNA-1
chr5_2	exon	30475801	30475867	30475801	ID=Msa0767410-mRNA-1:exon:11477;Parent=Msa0767410-mRNA-1
chr5_2	exon	30476619	30476733	30476619	ID=Msa0767410-mRNA-1:exon:11478;Parent=Msa0767410-mRNA-1
chr5_2	exon	30476836	30476954	30476836	ID=Msa0767410-mRNA-1:exon:11479;Parent=Msa0767410-mRNA-1
chr5_2	exon	30477047	30477198	30477047	ID=Msa0767410-mRNA-1:exon:11480;Parent=Msa0767410-mRNA-1
chr5_2	exon	30478205	30478472	30478205	ID=Msa0767410-mRNA-1:exon:11481;Parent=Msa0767410-mRNA-1
chr5_2	exon	30478569	30478636	30478569	ID=Msa0767410-mRNA-1:exon:11482;Parent=Msa0767410-mRNA-1
chr5_2	exon	30478742	30478872	30478742	ID=Msa0767410-mRNA-1:exon:11483;Parent=Msa0767410-mRNA-1
chr5_2	five_prime_UTR	30475477	30475716	30475477	ID=Msa0767410-mRNA-1:five_prime_utr;Parent=Msa0767410-mRNA-1
chr5_2	five_prime_UTR	30475801	30475867	30475801	ID=Msa0767410-mRNA-1:five_prime_utr;Parent=Msa0767410-mRNA-1
chr5_2	five_prime_UTR	30476619	30476733	30476619	ID=Msa0767410-mRNA-1:five_prime_utr;Parent=Msa0767410-mRNA-1
chr5_2	five_prime_UTR	30476836	30476941	30476836	ID=Msa0767410-mRNA-1:five_prime_utr;Parent=Msa0767410-mRNA-1
chr5_2	CDS	30476942	30476954	30476942	ID=Msa0767410-mRNA-1:cds;Parent=Msa0767410-mRNA-1
chr5_2	CDS	30477047	30477198	30477047	ID=Msa0767410-mRNA-1:cds;Parent=Msa0767410-mRNA-1
chr5_2	CDS	30478205	30478472	30478205	ID=Msa0767410-mRNA-1:cds;Parent=Msa0767410-mRNA-1
chr5_2	CDS	30478569	30478636	30478569	ID=Msa0767410-mRNA-1:cds;Parent=Msa0767410-mRNA-1
chr5_2	CDS	30478742	30478789	30478742	ID=Msa0767410-mRNA-1:cds;Parent=Msa0767410-mRNA-1
chr5_2	three_prime_UTR	30478790	30478872	30478790	ID=Msa0767410-mRNA-1:three_prime_utr;Parent=Msa0767410-mRNA-1

Gene Sequence

>Msa0767410

ATGGCATCCAAAGGAAAAGCTCCAAAGCTCACCAAGTTTGCAGACATTGTTGGTGGAAGGATTGACTTCCAGATCCAGGGTAGGGTTATCAATCTTTGGACTACTCCTGACCGTTTCAACGCAGCTGAAGCTGGAAGTATGCACATGATTTTGCTGGATAATCAGGGTGTGACAATCCATGCAAGTGTCAGGAAGCATTTGGTTCCTATATTTAAGGATCAGTTTGAGGAAGGGTCCATTTATGTTATTGAGAAGCTTATGGTTGCACAGAATGATCCAACATTTAAGACTACTCCCCATAAGTACAAACTGAATCTTATGAGAGGTACCACTATTTTCAAGCTCAAGGATTCCAATGAAATCCCTAAGAGACATTTTCAATTCATGTCTTTCCTTGAAATCATGGCCTGCCACAAGGAGGATCGCTTGATAGATGTCATTGGTCATGTCGTTGAGAAAGATGAACTGAAAGTGACTGAGAAAAATGGGAAGACCAACAAGGGGAAATCGAATTCACTGCGGTTTCTTACCGATGTGATCTTGGAATGA

Protein sequence

>Msa0767410

MASKGKAPKLTKFADIVGGRIDFQIQGRVINLWTTPDRFNAAEAGSMHMILLDNQGVTIHASVRKHLVPIFKDQFEEGSIYVIEKLMVAQNDPTFKTTPHKYKLNLMRGTTIFKLKDSNEIPKRHFQFMSFLEIMACHKEDRLIDVIGHVVEKDELKVTEKNGKTNKGKSNSLRFLTDVILE*