Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0772760 | sp|Q39193|SRK2I_ARATH | 81.766 | 351 | 62 | 2 | 7 | 355 | 11 | 361 | 0.0 | 582 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0772760 | A0A072UDL3 | 99.155 | 355 | 3 | 0 | 1 | 355 | 1 | 355 | 0.0 | 731 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0054110 | Msa0772760 | -0.803139 | 4.019250e-49 | -8.615850e-47 |
| Msa0375930 | Msa0772760 | -0.808323 | 3.276719e-50 | -8.615850e-47 |
| Msa0424570 | Msa0772760 | -0.808034 | 3.776607e-50 | -8.615850e-47 |
| Msa0516000 | Msa0772760 | -0.807186 | 5.716837e-50 | -8.615850e-47 |
| Msa0658920 | Msa0772760 | -0.824767 | 6.753659e-54 | -8.615850e-47 |
| Msa0704350 | Msa0772760 | -0.821578 | 3.748304e-53 | -8.615850e-47 |
| Msa0704430 | Msa0772760 | -0.828775 | 7.452154e-55 | -8.615850e-47 |
| Msa0707870 | Msa0772760 | -0.802228 | 6.196514e-49 | -8.615850e-47 |
| Msa0715390 | Msa0772760 | 0.951529 | 1.686878e-109 | -8.615850e-47 |
| Msa0772760 | Msa0798300 | 0.971952 | 5.578713e-134 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0772760 | MtrunA17_Chr5g0402661 | 99.155 | 355 | 3 | 0 | 1 | 355 | 1 | 355 | 0.0 | 731 |
| Msa0772760 | MtrunA17_Chr8g0374571 | 84.874 | 357 | 52 | 2 | 1 | 355 | 6 | 362 | 0.0 | 631 |
| Msa0772760 | MtrunA17_Chr4g0045371 | 79.944 | 354 | 68 | 3 | 5 | 355 | 10 | 363 | 0.0 | 591 |
| Msa0772760 | MtrunA17_Chr1g0162301 | 79.037 | 353 | 70 | 2 | 7 | 355 | 13 | 365 | 0.0 | 578 |
| Msa0772760 | MtrunA17_Chr4g0055451 | 68.358 | 335 | 102 | 2 | 16 | 350 | 3 | 333 | 1.39e-177 | 496 |
| Msa0772760 | MtrunA17_Chr2g0277501 | 70.625 | 320 | 92 | 2 | 16 | 333 | 2 | 321 | 1.99e-169 | 476 |
| Msa0772760 | MtrunA17_Chr5g0426521 | 67.359 | 337 | 107 | 2 | 16 | 352 | 2 | 335 | 2.55e-169 | 475 |
| Msa0772760 | MtrunA17_Chr8g0381821 | 71.061 | 311 | 88 | 2 | 16 | 325 | 2 | 311 | 2.32e-167 | 470 |
| Msa0772760 | MtrunA17_Chr3g0131061 | 72.425 | 301 | 82 | 1 | 16 | 315 | 2 | 302 | 2.45e-167 | 471 |
| Msa0772760 | MtrunA17_Chr8g0385281 | 71.429 | 301 | 85 | 1 | 16 | 315 | 3 | 303 | 1.41e-166 | 469 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0772760 | AT5G66880.1 | 81.766 | 351 | 62 | 2 | 7 | 355 | 11 | 361 | 0.0 | 582 |
| Msa0772760 | AT5G66880.2 | 81.766 | 351 | 62 | 2 | 7 | 355 | 11 | 361 | 0.0 | 582 |
| Msa0772760 | AT3G50500.1 | 79.202 | 351 | 71 | 2 | 7 | 355 | 12 | 362 | 0.0 | 566 |
| Msa0772760 | AT4G33950.1 | 79.320 | 353 | 69 | 3 | 7 | 355 | 10 | 362 | 0.0 | 566 |
| Msa0772760 | AT4G33950.2 | 80.734 | 327 | 62 | 1 | 7 | 332 | 10 | 336 | 0.0 | 561 |
| Msa0772760 | AT3G50500.2 | 76.860 | 363 | 75 | 3 | 2 | 355 | 7 | 369 | 0.0 | 560 |
| Msa0772760 | AT5G63650.1 | 72.131 | 305 | 84 | 1 | 16 | 319 | 2 | 306 | 1.76e-167 | 471 |
| Msa0772760 | AT1G10940.1 | 70.764 | 301 | 87 | 1 | 16 | 315 | 2 | 302 | 2.82e-166 | 468 |
| Msa0772760 | AT5G08590.1 | 71.096 | 301 | 87 | 0 | 16 | 316 | 2 | 302 | 1.10e-165 | 466 |
| Msa0772760 | AT1G60940.1 | 70.861 | 302 | 87 | 1 | 16 | 316 | 2 | 303 | 5.15e-165 | 465 |
Find 110 sgRNAs with CRISPR-Local
Find 278 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGAAATTACCTCTGC+AGG | 0.384298 | 5_2:+47107687 | None:intergenic |
| AAACTGAAAAGAGAGCTTTA+TGG | 0.373802 | 5_2:-47106554 | Msa0772760:three_prime_UTR |
| AAAGTCAACTGTGGGAACTC+CGG | 0.576196 | 5_2:-47108286 | Msa0772760:CDS |
| AAATAGAAATTACCTCTGCA+GGG | 0.556900 | 5_2:+47107688 | None:intergenic |
| AACCTTATACGTGATGCTAG+TGG | 0.588663 | 5_2:-47107942 | Msa0772760:CDS |
| AACTTGATGTAGATAGCAGT+GGG | 0.529690 | 5_2:-47106619 | Msa0772760:CDS |
| AAGCTACCGTACCAGCAGCT+GGG | 0.608355 | 5_2:-47106712 | Msa0772760:CDS |
| AATGAACGTTATCCTCACTG+AGG | 0.789511 | 5_2:+47106163 | None:intergenic |
| ACCTTATACGTGATGCTAGT+GGG | 0.557038 | 5_2:-47107941 | Msa0772760:CDS |
| ACTTCTGGGGCAATATAAGC+CGG | 0.591957 | 5_2:+47108267 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATCTCGAACAAAATCGTAA+CGG | + | chr5_2:47106306-47106325 | None:intergenic | 30.0% | |
| TATATCAAATTGGGTTAACG+AGG | - | chr5_2:47106951-47106970 | Msa0772760:intron | 30.0% | |
| AGAGAGAAATAATCAATCAC+AGG | - | chr5_2:47107236-47107255 | Msa0772760:intron | 30.0% | |
| TCCTAATATTGTCAGGTTTA+AGG | - | chr5_2:47107270-47107289 | Msa0772760:intron | 30.0% | |
| TCCTTAAACCTGACAATATT+AGG | + | chr5_2:47107274-47107293 | None:intergenic | 30.0% | |
| TAATATTGTCAGGTTTAAGG+AGG | - | chr5_2:47107273-47107292 | Msa0772760:intron | 30.0% | |
| TCTAAATCCTTCATGTCAAA+AGG | - | chr5_2:47107315-47107334 | Msa0772760:intron | 30.0% | |
| AGATGCATATTCCATTACAA+TGG | + | chr5_2:47107410-47107429 | None:intergenic | 30.0% | |
| AATTCTTCTCAAAGAATGAG+AGG | + | chr5_2:47107518-47107537 | None:intergenic | 30.0% | |
| AGATGCAAAAAGTAATTTGG+AGG | + | chr5_2:47108060-47108079 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 47106069 | 47110683 | 47106069 | ID=Msa0772760;Name=Msa0772760 |
| chr5_2 | mRNA | 47106069 | 47110683 | 47106069 | ID=Msa0772760-mRNA-1;Parent=Msa0772760;Name=Msa0772760-mRNA-1;_AED=0.15;_eAED=0.15;_QI=187|1|1|1|1|1|9|529|355 |
| chr5_2 | exon | 47106069 | 47106882 | 47106069 | ID=Msa0772760-mRNA-1:exon:14166;Parent=Msa0772760-mRNA-1 |
| chr5_2 | exon | 47107701 | 47107799 | 47107701 | ID=Msa0772760-mRNA-1:exon:14165;Parent=Msa0772760-mRNA-1 |
| chr5_2 | exon | 47107886 | 47107990 | 47107886 | ID=Msa0772760-mRNA-1:exon:14164;Parent=Msa0772760-mRNA-1 |
| chr5_2 | exon | 47108239 | 47108331 | 47108239 | ID=Msa0772760-mRNA-1:exon:14163;Parent=Msa0772760-mRNA-1 |
| chr5_2 | exon | 47108871 | 47108963 | 47108871 | ID=Msa0772760-mRNA-1:exon:14162;Parent=Msa0772760-mRNA-1 |
| chr5_2 | exon | 47109114 | 47109167 | 47109114 | ID=Msa0772760-mRNA-1:exon:14161;Parent=Msa0772760-mRNA-1 |
| chr5_2 | exon | 47109269 | 47109370 | 47109269 | ID=Msa0772760-mRNA-1:exon:14160;Parent=Msa0772760-mRNA-1 |
| chr5_2 | exon | 47109458 | 47109532 | 47109458 | ID=Msa0772760-mRNA-1:exon:14159;Parent=Msa0772760-mRNA-1 |
| chr5_2 | exon | 47110335 | 47110683 | 47110335 | ID=Msa0772760-mRNA-1:exon:14158;Parent=Msa0772760-mRNA-1 |
| chr5_2 | five_prime_UTR | 47110497 | 47110683 | 47110497 | ID=Msa0772760-mRNA-1:five_prime_utr;Parent=Msa0772760-mRNA-1 |
| chr5_2 | CDS | 47110335 | 47110496 | 47110335 | ID=Msa0772760-mRNA-1:cds;Parent=Msa0772760-mRNA-1 |
| chr5_2 | CDS | 47109458 | 47109532 | 47109458 | ID=Msa0772760-mRNA-1:cds;Parent=Msa0772760-mRNA-1 |
| chr5_2 | CDS | 47109269 | 47109370 | 47109269 | ID=Msa0772760-mRNA-1:cds;Parent=Msa0772760-mRNA-1 |
| chr5_2 | CDS | 47109114 | 47109167 | 47109114 | ID=Msa0772760-mRNA-1:cds;Parent=Msa0772760-mRNA-1 |
| chr5_2 | CDS | 47108871 | 47108963 | 47108871 | ID=Msa0772760-mRNA-1:cds;Parent=Msa0772760-mRNA-1 |
| chr5_2 | CDS | 47108239 | 47108331 | 47108239 | ID=Msa0772760-mRNA-1:cds;Parent=Msa0772760-mRNA-1 |
| chr5_2 | CDS | 47107886 | 47107990 | 47107886 | ID=Msa0772760-mRNA-1:cds;Parent=Msa0772760-mRNA-1 |
| chr5_2 | CDS | 47107701 | 47107799 | 47107701 | ID=Msa0772760-mRNA-1:cds;Parent=Msa0772760-mRNA-1 |
| chr5_2 | CDS | 47106598 | 47106882 | 47106598 | ID=Msa0772760-mRNA-1:cds;Parent=Msa0772760-mRNA-1 |
| chr5_2 | three_prime_UTR | 47106069 | 47106597 | 47106069 | ID=Msa0772760-mRNA-1:three_prime_utr;Parent=Msa0772760-mRNA-1 |
| Gene Sequence |
>Msa0772760 ATGAATCCTCCTCCAACCATGGATATGCCGATCATGCACGACAGCGACCGTTACGATTTTGTTCGAGATATTGGATCAGGGAATTTCGGTGTAGCACGACTCATGAGAGATAAACACACCAAAGAACTTGTTGCTGTCAAGTATATCGAACGTGGTGATAAGATTGATGAAAATGTTAAGAGAGAAATAATCAATCACAGGTCTTTGAGACATCCTAATATTGTCAGGTTTAAGGAGGTCATTTTAACACCTACGCATCTGGCCATTGTAATGGAATATGCATCTGGAGGAGAAATGTTTGAAAGAATCAGCAGAGCAGGACGTTTTACTGAGGATGAGGCTCGTTTCTTCTTTCAACAGTTGATATCCGGTGTCAGCTATTGTCATTCAATGCAAGTGTGTCACCGGGATCTGAAGTTGGAAAACACTTTGTTGGATGGAGATCCAGCACTTCATTTGAAGATCTGTGATTTTGGATACTCCAAGTCTTCGGTTCTTCATTCACAACCAAAGTCAACTGTGGGAACTCCGGCTTATATTGCCCCAGAAGTACTGCTGAAGCAAGAGTATGATGGAAAGGTTGCAGATGTATGGTCATGTGGAGTAACCTTATACGTGATGCTAGTGGGATCATATCCTTTTGAAGATCCCGATAATCCAAAGGACTTCCGGAAGACAATTCAGAGGGTTCTCAGTGTCCAGTATTCCGTTCCAGACTTTGTTCAAATATCTCCTGAATGTCGTGAAGTTATATCAAGAATCTTTGTTTTTGACCCTGCAGAGAGAATCACCATTCCAGAAATATTGAAAAATGAATGGTTTCTGAAGAATCTTCCTGCTGACTTGGTGAATGAAAAGTTAACGGATAACCAATTTGAAGAGCCAGATCAGCCCATGCAGAGCATAGATACAATCATGCAGATCATTTCAGAAGCTACCGTACCAGCAGCTGGGAGCTATTTAGACCAGTTTATGCCTGATAATGATACGGATGATGATATGGATGATATGGACTCTGACTATGAACTTGATGTAGATAGCAGTGGGGAGATTGTATATGCCATATAA |
| Protein sequence |
>Msa0772760 MNPPPTMDMPIMHDSDRYDFVRDIGSGNFGVARLMRDKHTKELVAVKYIERGDKIDENVKREIINHRSLRHPNIVRFKEVILTPTHLAIVMEYASGGEMFERISRAGRFTEDEARFFFQQLISGVSYCHSMQVCHRDLKLENTLLDGDPALHLKICDFGYSKSSVLHSQPKSTVGTPAYIAPEVLLKQEYDGKVADVWSCGVTLYVMLVGSYPFEDPDNPKDFRKTIQRVLSVQYSVPDFVQISPECREVISRIFVFDPAERITIPEILKNEWFLKNLPADLVNEKLTDNQFEEPDQPMQSIDTIMQIISEATVPAAGSYLDQFMPDNDTDDDMDDMDSDYELDVDSSGEIVYAI* |
