AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1343540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1343540 MtrunA17_Chr8g0384911 98.344 604 10 0 11 614 139 742 0.0 1229
Msa1343540 MtrunA17_Chr8g0384911 33.537 164 95 6 229 381 107 267 5.72e-14 75.5
Msa1343540 MtrunA17_Chr1g0193701 26.768 198 112 6 209 377 71 264 4.23e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1343540 AT5G08720.1 69.508 610 160 10 11 614 129 718 0.0 817
Msa1343540 AT5G08720.1 34.483 145 87 4 229 367 97 239 9.51e-14 75.1
Msa1343540 AT4G01650.2 32.934 167 88 6 229 373 38 202 1.01e-17 82.8
Msa1343540 AT4G01650.1 32.934 167 88 6 229 373 115 279 4.51e-17 82.4
Msa1343540 AT4G01650.3 36.449 107 59 3 229 326 115 221 2.79e-14 73.2

Find 204 sgRNAs with CRISPR-Local

Find 302 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTACTGATTGCTAAATT+TGG 0.126575 tig0006451:-80651 None:intergenic
CAGGAAATTGTGATGCTTTC+CGG 0.135896 tig0006451:-81475 None:intergenic
AAAACTTCTTTGGTTGTTAA+TGG 0.150554 tig0006451:+80090 Msa1343540:CDS
TAAAAGAGTTGGACATAAAT+TGG 0.162386 tig0006451:+82499 Msa1343540:CDS
TCCTTTCACTTGGCGCATTT+TGG 0.166058 tig0006451:+82796 Msa1343540:three_prime_UTR
ATAAATATATTTATACATTT+TGG 0.167927 tig0006451:+82544 Msa1343540:three_prime_UTR
AACAGCAATTGGGGTATATT+TGG 0.184340 tig0006451:+80234 Msa1343540:CDS
AAGCTATGGTTAATCAATTA+TGG 0.203967 tig0006451:-79035 None:intergenic
GCCAAAACAACTTATGAAAA+TGG 0.207859 tig0006451:+79126 Msa1343540:five_prime_UTR
TAGGACTTCAGTTCAAATTA+AGG 0.215602 tig0006451:+82841 Msa1343540:three_prime_UTR
CATTGAAGCTCGTGTTGTTT+TGG 0.228996 tig0006451:+79435 Msa1343540:CDS
TATTCAAATTTCCACCTAAA+TGG 0.230001 tig0006451:-78818 None:intergenic
TACTGATGCCATGTACTTAT+TGG 0.234172 tig0006451:+82763 Msa1343540:three_prime_UTR
CATATTCAGCAACAAATTTC+AGG 0.244825 tig0006451:-81673 None:intergenic
ATTTCCACCTAAATGGCTTC+TGG 0.245633 tig0006451:-78811 None:intergenic
CGACTTGCATAAAACTTCTT+TGG 0.246522 tig0006451:+80080 Msa1343540:CDS
AATTTCAGCTGTGGGATTAT+AGG 0.255058 tig0006451:-82958 None:intergenic
CTGAAATTTGTTGCTGAATA+TGG 0.261458 tig0006451:+81674 Msa1343540:CDS
AATCTTATCATATGACTCTT+TGG 0.263616 tig0006451:-79272 None:intergenic
TCGACTCATTCCATGGAAAA+TGG 0.265972 tig0006451:+81034 Msa1343540:CDS
TTAACCATAGCTTCATTATA+AGG 0.267969 tig0006451:+79045 Msa1343540:five_prime_UTR
TAGAGTTGAAAGGAATCTTT+TGG 0.269880 tig0006451:+80029 Msa1343540:CDS
GCCCATTTCCTGGAAGGATA+TGG 0.274340 tig0006451:+79374 Msa1343540:CDS
TTAGGTGGAAATTTGAATAA+TGG 0.274468 tig0006451:+78822 Msa1343540:five_prime_UTR
GTGAGCTTCAAGTGGTTTCT+TGG 0.276863 tig0006451:+78889 Msa1343540:five_prime_UTR
CTTCATTATAAGGGCATGTT+TGG 0.282710 tig0006451:+79055 Msa1343540:five_prime_UTR
GGTTTGGACACTTCAATTTC+CGG 0.285305 tig0006451:+80183 Msa1343540:CDS
TCTACTAGCATAAACTCTTT+AGG 0.285422 tig0006451:+79096 Msa1343540:five_prime_UTR
AACCATATCCTTCCAGGAAA+TGG 0.286044 tig0006451:-79376 None:intergenic
CCGCAGATTTGATGGACTTT+TGG 0.287220 tig0006451:+80305 Msa1343540:CDS
TTTATGTGAAACCGACAAAT+TGG 0.299097 tig0006451:+80140 Msa1343540:CDS
CGTATTCCTTGCCCATTTCC+TGG 0.303078 tig0006451:+79364 Msa1343540:CDS
TGCTCTAACCATATCCTTCC+AGG 0.304191 tig0006451:-79382 None:intergenic
TTAAGAAGTTTGAGGGAAAA+TGG 0.304395 tig0006451:+79765 Msa1343540:CDS
CCTTACCTCCAAACAAGATT+TGG 0.309840 tig0006451:-79008 None:intergenic
ACCGCAAACCAAAGGGTTAC+TGG 0.312478 tig0006451:+81828 Msa1343540:CDS
TAGGTGGAAATTTGAATAAT+GGG 0.318321 tig0006451:+78823 Msa1343540:five_prime_UTR
CACCCTTCAATAGCAAAATA+TGG 0.319903 tig0006451:+82713 Msa1343540:three_prime_UTR
TTGTTGTTGATGATGTTTCA+TGG 0.321467 tig0006451:-78593 None:intergenic
ATTTCATACCAAATCTTGTT+TGG 0.323525 tig0006451:+79000 Msa1343540:five_prime_UTR
GCCAAGGTTGTTGTCCTGTC+TGG 0.332172 tig0006451:-82370 None:intergenic
CCAGTGAGTTAAACAGCAAT+TGG 0.332368 tig0006451:+80223 Msa1343540:CDS
ATCAACTAATTTATCTTATG+AGG 0.337577 tig0006451:+79915 Msa1343540:CDS
GTTCATCTCCGCAGATTTGA+TGG 0.338069 tig0006451:+80297 Msa1343540:CDS
CAATAAAGTCCGCATTCTTC+AGG 0.340923 tig0006451:+80689 Msa1343540:CDS
CTTATTGCACACAAGTTTGA+TGG 0.342584 tig0006451:-81437 None:intergenic
TGCAAACCCAGAAGCCATTT+AGG 0.347976 tig0006451:+78804 Msa1343540:five_prime_UTR
TAATCAAAGGTCAAGAGTTC+CGG 0.348104 tig0006451:+81613 Msa1343540:CDS
CCCAGTAACCCTTTGGTTTG+CGG 0.349921 tig0006451:-81829 None:intergenic
TTTCCACCTAAATGGCTTCT+GGG 0.357536 tig0006451:-78810 None:intergenic
AGCTAGAGAGAGGTTCATTA+TGG 0.363567 tig0006451:-81798 None:intergenic
GTGTAAAGGCCTGCTTTATA+TGG 0.365800 tig0006451:+80933 Msa1343540:CDS
TCCTTGCCTTGAGAGATAGA+AGG 0.366056 tig0006451:-82338 None:intergenic
CTTTGATTTCGTATAGAACT+TGG 0.371993 tig0006451:-79229 None:intergenic
CCAAATCTTGTTTGGAGGTA+AGG 0.373331 tig0006451:+79008 Msa1343540:five_prime_UTR
TATGCAAGTCGTCTTCTGGC+TGG 0.376831 tig0006451:-80068 None:intergenic
GGACAATCTTGAAAATTTGC+AGG 0.376858 tig0006451:+81850 Msa1343540:CDS
AGGACTTCAGTTCAAATTAA+GGG 0.380996 tig0006451:+82842 Msa1343540:three_prime_UTR
CTTTGATATTGCACGGGCGC+TGG 0.383854 tig0006451:+82284 Msa1343540:CDS
GTATTCTGCTCACAAACTTC+AGG 0.385344 tig0006451:-81494 None:intergenic
TAACCATAGCTTCATTATAA+GGG 0.389994 tig0006451:+79046 Msa1343540:five_prime_UTR
GGAAAATGGTCTGTAAAATC+TGG 0.393706 tig0006451:+79779 Msa1343540:CDS
CGACAAATTGGCACCTGGTC+AGG 0.397683 tig0006451:+80152 Msa1343540:CDS
CAGTGAGTTAAACAGCAATT+GGG 0.406091 tig0006451:+80224 Msa1343540:CDS
TGCCTATGAGGAAGCAGCTT+CGG 0.407535 tig0006451:+81711 Msa1343540:CDS
ATCCTTTCCAAGAAAATAGC+TGG 0.407564 tig0006451:-79964 None:intergenic
AAGGAAGAGCGTAGAGAAAG+TGG 0.409251 tig0006451:-78696 None:intergenic
ATGCTGATATTAACTCCGTT+TGG 0.413462 tig0006451:+78946 Msa1343540:five_prime_UTR
GTTCCTTTCTGAAGCTATCA+TGG 0.414791 tig0006451:+81128 Msa1343540:CDS
ATGCAAGTCGTCTTCTGGCT+GGG 0.415764 tig0006451:-80067 None:intergenic
AATCAAAGGTCAAGAGTTCC+GGG 0.421005 tig0006451:+81614 Msa1343540:CDS
AAAAGAGTTGGACATAAATT+GGG 0.422170 tig0006451:+82500 Msa1343540:CDS
GAACAACAAATTATATCATC+TGG 0.426503 tig0006451:+81524 Msa1343540:CDS
TCGTTCAAATGACTTTCTAC+TGG 0.427969 tig0006451:-82105 None:intergenic
TTAATTTGAACTGAAGTCCT+AGG 0.430865 tig0006451:-82839 None:intergenic
TATCTACATCTGCTGATTCT+CGG 0.432007 tig0006451:-82413 None:intergenic
GATTTCAAAGGGCAATGTAT+TGG 0.434318 tig0006451:+79410 Msa1343540:CDS
AAATGATGGTTTGGAATGTA+AGG 0.434736 tig0006451:-78715 None:intergenic
GGAGACTTTAAGAAGTTTGA+GGG 0.435260 tig0006451:+79758 Msa1343540:CDS
AAGCTCCTGTTCGTGATGTA+TGG 0.435936 tig0006451:+80467 Msa1343540:CDS
TCAAGTGTTTATCATATAAG+TGG 0.440789 tig0006451:-79183 None:intergenic
TAAGTAGATTTCAGAGATGC+TGG 0.441100 tig0006451:+82060 Msa1343540:CDS
AGAATGTGCTTACAGAATTG+AGG 0.448020 tig0006451:-79471 None:intergenic
ACCATATCCTTCCAGGAAAT+GGG 0.452989 tig0006451:-79375 None:intergenic
TTTCTACTGGGCATAAATGA+TGG 0.458102 tig0006451:-82092 None:intergenic
CTTGGAAAGGATTGTCAGAT+CGG 0.461197 tig0006451:+79975 Msa1343540:CDS
TAAGTACAACTATGTGTTGA+TGG 0.461654 tig0006451:+82569 Msa1343540:three_prime_UTR
AGATTGTCCCAGTAACCCTT+TGG 0.463671 tig0006451:-81836 None:intergenic
TGAGTGTGAGTGTGTGAAGA+AGG 0.463920 tig0006451:-78629 None:intergenic
CCAATTGCTGTTTAACTCAC+TGG 0.464061 tig0006451:-80223 None:intergenic
TGGTCAGGATAAAGAAGGTT+TGG 0.466478 tig0006451:+80167 Msa1343540:CDS
GAGACAGACTATTTAATCCT+AGG 0.471771 tig0006451:+82822 Msa1343540:three_prime_UTR
CTGACCAGGTGCCAATTTGT+CGG 0.477873 tig0006451:-80151 None:intergenic
TTCTGAAGCTATCATGGAAG+AGG 0.480831 tig0006451:+81134 Msa1343540:CDS
TGGAGACTTTAAGAAGTTTG+AGG 0.481726 tig0006451:+79757 Msa1343540:CDS
TTGCACGGGCGCTGGAAAAG+TGG 0.485341 tig0006451:+82292 Msa1343540:CDS
TGCACGGGCGCTGGAAAAGT+GGG 0.485874 tig0006451:+82293 Msa1343540:CDS
TTGGAGGGAACGGAGAGTTA+AGG 0.486180 tig0006451:+78908 Msa1343540:five_prime_UTR
AGCCGAAGCTGCTTCCTCAT+AGG 0.486988 tig0006451:-81713 None:intergenic
TACAAATACCGCAAACCAAA+GGG 0.487175 tig0006451:+81821 Msa1343540:CDS
GGAAGACGCAGGCAATGAAC+CGG 0.492642 tig0006451:-80202 None:intergenic
TAAGAGACTACGTTGAGAAC+CGG 0.494267 tig0006451:+81456 Msa1343540:CDS
CCGCAAACCAAAGGGTTACT+GGG 0.494866 tig0006451:+81829 Msa1343540:CDS
ACATTCCATACATCACGAAC+AGG 0.495980 tig0006451:-80472 None:intergenic
TCCAAAATGCGCCAAGTGAA+AGG 0.498924 tig0006451:-82797 None:intergenic
CAGGCCGCTTTGATATTGCA+CGG 0.501731 tig0006451:+82277 Msa1343540:intron
CAAATTATATCATCTGGCTC+TGG 0.502999 tig0006451:+81530 Msa1343540:CDS
TTCCTTGCCCATTTCCTGGA+AGG 0.505510 tig0006451:+79368 Msa1343540:CDS
ATAGCTGGGAAATTGAATCT+TGG 0.507663 tig0006451:-79949 None:intergenic
TGCAAGTCGTCTTCTGGCTG+GGG 0.510055 tig0006451:-80066 None:intergenic
TCATACCAAATCTTGTTTGG+AGG 0.510529 tig0006451:+79003 Msa1343540:five_prime_UTR
AGTGCGAGCATTGTGGCCAC+TGG 0.511613 tig0006451:-78550 None:intergenic
CCTTTCAACTCTATATGCTA+AGG 0.516881 tig0006451:-80019 None:intergenic
CGTTCAAATGACTTTCTACT+GGG 0.520909 tig0006451:-82104 None:intergenic
CTATACGAAATCAAAGTCAT+TGG 0.522063 tig0006451:+79236 Msa1343540:five_prime_UTR
AAGTGGTTTCTTGGAGGGAA+CGG 0.523263 tig0006451:+78898 Msa1343540:five_prime_UTR
CAATAGCAAAATATGGCTGC+TGG 0.524984 tig0006451:+82720 Msa1343540:three_prime_UTR
AGAAAGTCATTTGAACGAGT+TGG 0.530001 tig0006451:+82110 Msa1343540:CDS
AGGAAGCAGCTTCGGCTGCA+TGG 0.532690 tig0006451:+81719 Msa1343540:CDS
TCAATATCCCTCTGTAGTCC+CGG 0.534477 tig0006451:-81632 None:intergenic
AGCTTCAAGTGGTTTCTTGG+AGG 0.534532 tig0006451:+78892 Msa1343540:five_prime_UTR
GGGTTTGCACTGTGTTGTTG+AGG 0.536639 tig0006451:-78790 None:intergenic
GCTAAGGCTCGTAGATTCAC+AGG 0.538658 tig0006451:-80003 None:intergenic
TGAGAAGGCTATAACTCGCA+TGG 0.539683 tig0006451:+81754 Msa1343540:CDS
TTCAAAGCTCATCTAATCAA+AGG 0.543340 tig0006451:+81600 Msa1343540:CDS
AAACCTTCTTTATCCTGACC+AGG 0.545502 tig0006451:-80165 None:intergenic
GCTTCAAGTGGTTTCTTGGA+GGG 0.545578 tig0006451:+78893 Msa1343540:five_prime_UTR
GCATACAGAACTAAAAGAGT+TGG 0.545618 tig0006451:+82488 Msa1343540:CDS
TTCTCGCCTTCTATCTCTCA+AGG 0.546788 tig0006451:+82332 Msa1343540:CDS
ATGCCCTTATAATGAAGCTA+TGG 0.547604 tig0006451:-79049 None:intergenic
AAGTAGATTTCAGAGATGCT+GGG 0.547739 tig0006451:+82061 Msa1343540:CDS
AGGCCGCTTTGATATTGCAC+GGG 0.548501 tig0006451:+82278 Msa1343540:intron
TTCTACTGGGCATAAATGAT+GGG 0.548996 tig0006451:-82091 None:intergenic
ATCAGTTAAAGCATTCCAAA+CGG 0.551914 tig0006451:-78961 None:intergenic
GGACTTCAGTTCAAATTAAG+GGG 0.555128 tig0006451:+82843 Msa1343540:three_prime_UTR
AATATGCAGGAAGGGTGTAA+AGG 0.555160 tig0006451:+80919 Msa1343540:intron
GGTGACTTCGACTCATTCCA+TGG 0.555479 tig0006451:+81027 Msa1343540:CDS
ACCAGACAGGACAACAACCT+TGG 0.555859 tig0006451:+82369 Msa1343540:CDS
TCTTGAAAATTTGCAGGACG+AGG 0.558044 tig0006451:+81856 Msa1343540:CDS
GTTTCATGGCATGTTGGCAT+AGG 0.558640 tig0006451:-78579 None:intergenic
AGTCATACAATTTCAGCTGT+GGG 0.559385 tig0006451:-82966 None:intergenic
AGGAAGGGTTTATGCCTATG+AGG 0.559853 tig0006451:+81699 Msa1343540:CDS
AGGAAGAGCGTAGAGAAAGT+GGG 0.562581 tig0006451:-78695 None:intergenic
GCACCTGGTCAGGATAAAGA+AGG 0.563894 tig0006451:+80162 Msa1343540:CDS
TAGAGCAAAGAGGATTTCAA+AGG 0.565114 tig0006451:+79398 Msa1343540:CDS
TTGCTGAATATGGTCAGGAA+GGG 0.565420 tig0006451:+81684 Msa1343540:CDS
GATGATGTTTCATGGCATGT+TGG 0.566768 tig0006451:-78585 None:intergenic
GTTGCTGAATATGGTCAGGA+AGG 0.566894 tig0006451:+81683 Msa1343540:CDS
ATTTGTTGCTGAATATGGTC+AGG 0.570102 tig0006451:+81679 Msa1343540:CDS
TTTCCCTCGCAATTTGCACA+AGG 0.570479 tig0006451:+78755 Msa1343540:five_prime_UTR
TAGCAAAATATGGCTGCTGG+TGG 0.574174 tig0006451:+82723 Msa1343540:three_prime_UTR
GGGTTAGCTCTTGAAGAAGA+AGG 0.578633 tig0006451:-78675 None:intergenic
CTTCCATGATAGCTTCAGAA+AGG 0.579834 tig0006451:-81131 None:intergenic
TCCTTTCCAAGAAAATAGCT+GGG 0.581774 tig0006451:-79963 None:intergenic
AGAGCAAAGAGGATTTCAAA+GGG 0.581778 tig0006451:+79399 Msa1343540:CDS
GTGCTAAGCAAGAATGTACA+AGG 0.582199 tig0006451:-82608 None:intergenic
CATGAAGCACCATATAAAGC+AGG 0.584729 tig0006451:-80942 None:intergenic
TATTGCTATGACAGTTTGCG+TGG 0.584981 tig0006451:+82924 Msa1343540:three_prime_UTR
GCCTTCTATCTCTCAAGGCA+AGG 0.588537 tig0006451:+82337 Msa1343540:CDS
TTCCCTCGCAATTTGCACAA+GGG 0.591926 tig0006451:+78756 Msa1343540:five_prime_UTR
TTACAAATACCGCAAACCAA+AGG 0.593624 tig0006451:+81820 Msa1343540:CDS
AAAGTGGGGTGGACTTCATG+AGG 0.593784 tig0006451:+82308 Msa1343540:CDS
GAGTCATACAATTTCAGCTG+TGG 0.594592 tig0006451:-82967 None:intergenic
TGCCCTTGTGCAAATTGCGA+GGG 0.594902 tig0006451:-78758 None:intergenic
ATCCTTCCAGGAAATGGGCA+AGG 0.601199 tig0006451:-79370 None:intergenic
AAAATTTACCAATAAGTACA+TGG 0.603191 tig0006451:-82771 None:intergenic
CTACAACACAAGGCTTGTCA+AGG 0.605033 tig0006451:-80269 None:intergenic
AGTAGATTTCAGAGATGCTG+GGG 0.605132 tig0006451:+82062 Msa1343540:CDS
GCTTCGGCTGCATGGAAGAG+TGG 0.605805 tig0006451:+81727 Msa1343540:CDS
GAGAAGGCTATAACTCGCAT+GGG 0.607086 tig0006451:+81755 Msa1343540:CDS
AGTGAGTTAAACAGCAATTG+GGG 0.607282 tig0006451:+80225 Msa1343540:CDS
AGCAAAATATGGCTGCTGGT+GGG 0.608587 tig0006451:+82724 Msa1343540:three_prime_UTR
GGTATTTGTAAGCTAGAGAG+AGG 0.610502 tig0006451:-81808 None:intergenic
TGTGTGTCTTGAGAAATATA+AGG 0.610890 tig0006451:-82459 None:intergenic
CCTTAGCATATAGAGTTGAA+AGG 0.613436 tig0006451:+80019 Msa1343540:CDS
AAGAGTTCCGGGACTACAGA+GGG 0.615046 tig0006451:+81625 Msa1343540:CDS
TGGAAGAGTGGATATTGAGA+AGG 0.615915 tig0006451:+81739 Msa1343540:CDS
AGGATATGGTTAGAGCAAAG+AGG 0.617625 tig0006451:+79388 Msa1343540:CDS
ATGCCCTTGTGCAAATTGCG+AGG 0.617760 tig0006451:-78759 None:intergenic
CGGGCGCTGGAAAAGTGGGG+TGG 0.622705 tig0006451:+82297 Msa1343540:CDS
GAAACCGACAAATTGGCACC+TGG 0.631692 tig0006451:+80147 Msa1343540:CDS
GCACGGGCGCTGGAAAAGTG+GGG 0.634461 tig0006451:+82294 Msa1343540:CDS
TTTCATGTGAAGGAAAATGG+AGG 0.635353 tig0006451:+80409 Msa1343540:intron
AAGGCTATAACTCGCATGGG+TGG 0.635453 tig0006451:+81758 Msa1343540:CDS
GGTTAACTGTGAGCTTCAAG+TGG 0.636625 tig0006451:+78881 Msa1343540:five_prime_UTR
TAAGCAAGAATGTACAAGGA+AGG 0.637170 tig0006451:-82604 None:intergenic
TTTCTCAAGACACACACAAG+TGG 0.642360 tig0006451:+82466 Msa1343540:CDS
CAAGAGTTCCGGGACTACAG+AGG 0.649469 tig0006451:+81624 Msa1343540:CDS
AGAAGAAGAAAGTGACCGTG+AGG 0.650924 tig0006451:+78860 Msa1343540:five_prime_UTR
GGTCTGTAAAATCTGGAACA+AGG 0.653193 tig0006451:+79786 Msa1343540:CDS
CCAAAAGTCCATCAAATCTG+CGG 0.656121 tig0006451:-80305 None:intergenic
GCTACAGAGTGCGAGCATTG+TGG 0.656834 tig0006451:-78557 None:intergenic
TATGGTGCTTCATGCTCGTG+TGG 0.656840 tig0006451:+80951 Msa1343540:CDS
GATTTGGTATGAAATCAGCT+AGG 0.657566 tig0006451:-78992 None:intergenic
TTAAGTTGCTTATACAAACA+CGG 0.659491 tig0006451:+79206 Msa1343540:five_prime_UTR
ACAGGACAACAACCTTGGCA+AGG 0.660733 tig0006451:+82374 Msa1343540:CDS
TGAACTTCATCTACAACACA+AGG 0.661750 tig0006451:-80279 None:intergenic
GAAGCTCACAGTTAACCTCA+CGG 0.685357 tig0006451:-78875 None:intergenic
TTTAACTCACTGGAAGACGC+AGG 0.696438 tig0006451:-80213 None:intergenic
AAACCCAGAAGCCATTTAGG+TGG 0.697351 tig0006451:+78807 Msa1343540:five_prime_UTR
TGGTCCGTACCTGAAGAATG+CGG 0.706706 tig0006451:-80698 None:intergenic
CACACTGCTAAAGTACTCTG+TGG 0.709210 tig0006451:+81083 Msa1343540:CDS
GCGCCCGTGCAATATCAAAG+CGG 0.728813 tig0006451:-82281 None:intergenic
GTAGATTTCAGAGATGCTGG+GGG 0.734657 tig0006451:+82063 Msa1343540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTTCAAAAAAAAAAAAA+AGG - tig0006451:81275-81294 None:intergenic 10.0%
!!! ATTTACTTTCTTTTTATAAA+AGG - tig0006451:81310-81329 None:intergenic 10.0%
!! TAGTTTATTTATATCATTCA+TGG - tig0006451:79851-79870 None:intergenic 15.0%
!!! CACATGAAAATATTTTTTAT+TGG - tig0006451:80398-80417 None:intergenic 15.0%
!!! TAAAAAATATTTTCATGTGA+AGG + tig0006451:80399-80418 Msa1343540:intron 15.0%
!! AAAAAAAAAAAAAGGAGATA+TGG - tig0006451:81267-81286 None:intergenic 15.0%
!!! ATTTTGTAGTATTAGAAAGT+TGG - tig0006451:79684-79703 None:intergenic 20.0%
!!! AAATTCTTTTTTAATGCTTC+AGG + tig0006451:79700-79719 Msa1343540:intron 20.0%
!!! GTATTAACTTTAAGAAACAT+AGG - tig0006451:79817-79836 None:intergenic 20.0%
!! ATCAACTAATTTATCTTATG+AGG + tig0006451:79915-79934 Msa1343540:CDS 20.0%
!! TCTATACTAGATTGTTTATT+AGG + tig0006451:80335-80354 Msa1343540:intron 20.0%
!!! ACAAATGTTTTTTTGTCAAT+TGG - tig0006451:80619-80638 None:intergenic 20.0%
!!! AATATGTATTTTACTAACAG+TGG - tig0006451:80827-80846 None:intergenic 20.0%
!!! ATACAAGTTCAAACTTTTAT+CGG - tig0006451:80871-80890 None:intergenic 20.0%
!! GTTTGTCTATAAATATGATT+TGG + tig0006451:81156-81175 Msa1343540:intron 20.0%
!!! TCTTTTTATAAAAGGAGATA+TGG - tig0006451:81302-81321 None:intergenic 20.0%
!!! AAGTAAATAACTGTTTTTGT+TGG + tig0006451:81322-81341 Msa1343540:intron 20.0%
!! AAAATATTGAAGTTGAGAAA+GGG - tig0006451:82150-82169 None:intergenic 20.0%
!!! TCTTACTACCATTTTAATTT+TGG + tig0006451:82225-82244 Msa1343540:intron 20.0%
!!! CTTACTACCATTTTAATTTT+GGG + tig0006451:82226-82245 Msa1343540:intron 20.0%
!! AAAATTTACCAATAAGTACA+TGG - tig0006451:82774-82793 None:intergenic 20.0%
!! TCATAAATGAGCAAAAAAAA+AGG + tig0006451:82881-82900 Msa1343540:three_prime_UTR 20.0%
!! CATAAATGAGCAAAAAAAAA+GGG + tig0006451:82882-82901 Msa1343540:three_prime_UTR 20.0%
! CAAAGATAAAAATGATGGTT+TGG - tig0006451:78727-78746 None:intergenic 25.0%
! AGACTCAAAGATAAAAATGA+TGG - tig0006451:78732-78751 None:intergenic 25.0%
! TATTCAAATTTCCACCTAAA+TGG - tig0006451:78821-78840 None:intergenic 25.0%
! TTAGGTGGAAATTTGAATAA+TGG + tig0006451:78822-78841 Msa1343540:five_prime_UTR 25.0%
! TAGGTGGAAATTTGAATAAT+GGG + tig0006451:78823-78842 Msa1343540:five_prime_UTR 25.0%
! ATTTCATACCAAATCTTGTT+TGG + tig0006451:79000-79019 Msa1343540:five_prime_UTR 25.0%
! AAGCTATGGTTAATCAATTA+TGG - tig0006451:79038-79057 None:intergenic 25.0%
! TTAACCATAGCTTCATTATA+AGG + tig0006451:79045-79064 Msa1343540:five_prime_UTR 25.0%
! TAACCATAGCTTCATTATAA+GGG + tig0006451:79046-79065 Msa1343540:five_prime_UTR 25.0%
!! ACCATTTTCATAAGTTGTTT+TGG - tig0006451:79130-79149 None:intergenic 25.0%
! TCAAGTGTTTATCATATAAG+TGG - tig0006451:79186-79205 None:intergenic 25.0%
!! TTAAGTTGCTTATACAAACA+CGG + tig0006451:79206-79225 Msa1343540:five_prime_UTR 25.0%
! AATCTTATCATATGACTCTT+TGG - tig0006451:79275-79294 None:intergenic 25.0%
!!! AAAAGTAGCAATTAGTTCTA+TGG + tig0006451:79535-79554 Msa1343540:intron 25.0%
! TTTAAAAACTGTATGAACAG+AGG + tig0006451:79617-79636 Msa1343540:intron 25.0%
! TTAAAAACTGTATGAACAGA+GGG + tig0006451:79618-79637 Msa1343540:intron 25.0%
!!! TTTTTTAATGCTTCAGGAAT+GGG + tig0006451:79706-79725 Msa1343540:intron 25.0%
! AAAACTTCTTTGGTTGTTAA+TGG + tig0006451:80090-80109 Msa1343540:CDS 25.0%
! TCTTCTGTCAAAAAGATAAA+TGG + tig0006451:80114-80133 Msa1343540:CDS 25.0%
! GTTGCCTTAATTCTTAAAAT+GGG + tig0006451:80357-80376 Msa1343540:intron 25.0%
!!! ACATCCCATTTTAAGAATTA+AGG - tig0006451:80364-80383 None:intergenic 25.0%
!! TATTTTCATGTGAAGGAAAA+TGG + tig0006451:80406-80425 Msa1343540:intron 25.0%
! TCAATATCTCTAGTGTAAAA+AGG - tig0006451:80542-80561 None:intergenic 25.0%
!! ATCTTACTGATTGCTAAATT+TGG - tig0006451:80654-80673 None:intergenic 25.0%
! TCTTGATTAAATCAATATGC+AGG + tig0006451:80906-80925 Msa1343540:intron 25.0%
! ATTAAATCAATATGCAGGAA+GGG + tig0006451:80911-80930 Msa1343540:intron 25.0%
!!! ATAACTGTTTTTGTTGGTAT+TGG + tig0006451:81328-81347 Msa1343540:intron 25.0%
!! TATTGGATACAATCTTTTGA+GGG + tig0006451:81345-81364 Msa1343540:intron 25.0%
! TAAATCGATCCACATTTAAA+TGG + tig0006451:81368-81387 Msa1343540:intron 25.0%
! GAACAACAAATTATATCATC+TGG + tig0006451:81524-81543 Msa1343540:CDS 25.0%
!!! TTAACTTTAGCTTGTTATTC+TGG - tig0006451:81920-81939 None:intergenic 25.0%
! CAAAATATTGAAGTTGAGAA+AGG - tig0006451:82151-82170 None:intergenic 25.0%
! TAAAAGAGTTGGACATAAAT+TGG + tig0006451:82499-82518 Msa1343540:CDS 25.0%
! AAAAGAGTTGGACATAAATT+GGG + tig0006451:82500-82519 Msa1343540:CDS 25.0%
! CAAATCAAGATTGAAAAAAG+AGG - tig0006451:82648-82667 None:intergenic 25.0%
!!! CTCTTTTTTCAATCTTGATT+TGG + tig0006451:82646-82665 Msa1343540:three_prime_UTR 25.0%
!! TAAATTTTCTTCCTTTCACT+TGG + tig0006451:82786-82805 Msa1343540:three_prime_UTR 25.0%
!! TTGTTGTTGATGATGTTTCA+TGG - tig0006451:78596-78615 None:intergenic 30.0%
! AAATGATGGTTTGGAATGTA+AGG - tig0006451:78718-78737 None:intergenic 30.0%
! ATCAGTTAAAGCATTCCAAA+CGG - tig0006451:78964-78983 None:intergenic 30.0%
TCTACTAGCATAAACTCTTT+AGG + tig0006451:79096-79115 Msa1343540:five_prime_UTR 30.0%
GCCAAAACAACTTATGAAAA+TGG + tig0006451:79126-79145 Msa1343540:five_prime_UTR 30.0%
! CTTTGATTTCGTATAGAACT+TGG - tig0006451:79232-79251 None:intergenic 30.0%
CTATACGAAATCAAAGTCAT+TGG + tig0006451:79236-79255 Msa1343540:five_prime_UTR 30.0%
!!! AGAGTTGTTTTTGTTGTTAG+TGG + tig0006451:79340-79359 Msa1343540:CDS 30.0%
!!! GAGTTGTTTTTGTTGTTAGT+GGG + tig0006451:79341-79360 Msa1343540:CDS 30.0%
!!! CTTTTTTAATGCTTCAGGAA+TGG + tig0006451:79705-79724 Msa1343540:intron 30.0%
!! TCGAGAACTTCATTTTTCAA+TGG + tig0006451:79730-79749 Msa1343540:CDS 30.0%
!! CTTCATTTTTCAATGGTTGA+TGG + tig0006451:79737-79756 Msa1343540:CDS 30.0%
TTAAGAAGTTTGAGGGAAAA+TGG + tig0006451:79765-79784 Msa1343540:CDS 30.0%
! TAGAGTTGAAAGGAATCTTT+TGG + tig0006451:80029-80048 Msa1343540:CDS 30.0%
! AGAGTTGAAAGGAATCTTTT+GGG + tig0006451:80030-80049 Msa1343540:CDS 30.0%
TTTATGTGAAACCGACAAAT+TGG + tig0006451:80140-80159 Msa1343540:CDS 30.0%
GGTTGCCTTAATTCTTAAAA+TGG + tig0006451:80356-80375 Msa1343540:intron 30.0%
! GACAAAAAAACATTTGTTGC+AGG + tig0006451:80622-80641 Msa1343540:intron 30.0%
!! TTACTAACAGTGGATTTTGA+TGG - tig0006451:80817-80836 None:intergenic 30.0%
GATTAAATCAATATGCAGGA+AGG + tig0006451:80910-80929 Msa1343540:intron 30.0%
! TTGTTTTCACTCGAAATGTT+TGG + tig0006451:81211-81230 Msa1343540:intron 30.0%
TGTCTTGCTTCAACTTATAT+GGG - tig0006451:81237-81256 None:intergenic 30.0%
! GTATTGGATACAATCTTTTG+AGG + tig0006451:81344-81363 Msa1343540:intron 30.0%
ACATTTCAGCCATTTAAATG+TGG - tig0006451:81380-81399 None:intergenic 30.0%
TGTTATAAGTTCTGTGTTTC+AGG + tig0006451:81397-81416 Msa1343540:intron 30.0%
TTCAAAGCTCATCTAATCAA+AGG + tig0006451:81600-81619 Msa1343540:CDS 30.0%
! ATTTCAGGAGTTCAGATTTT+AGG - tig0006451:81661-81680 None:intergenic 30.0%
CATATTCAGCAACAAATTTC+AGG - tig0006451:81676-81695 None:intergenic 30.0%
!! CTGAAATTTGTTGCTGAATA+TGG + tig0006451:81674-81693 Msa1343540:CDS 30.0%
TGCTAAGCCCAAAATTAAAA+TGG - tig0006451:82236-82255 None:intergenic 30.0%
!! AGATAATGGAATTAACTTGC+AGG + tig0006451:82258-82277 Msa1343540:intron 30.0%
TGTGTGTCTTGAGAAATATA+AGG - tig0006451:82462-82481 None:intergenic 30.0%
TAAGTACAACTATGTGTTGA+TGG + tig0006451:82569-82588 Msa1343540:three_prime_UTR 30.0%
!!! AGCCATATTTTGCTATTGAA+GGG - tig0006451:82718-82737 None:intergenic 30.0%
! TTAATTTGAACTGAAGTCCT+AGG - tig0006451:82842-82861 None:intergenic 30.0%
TAGGACTTCAGTTCAAATTA+AGG + tig0006451:82841-82860 Msa1343540:three_prime_UTR 30.0%
AGGACTTCAGTTCAAATTAA+GGG + tig0006451:82842-82861 Msa1343540:three_prime_UTR 30.0%
AATGAGCAAAAAAAAAGGGA+AGG + tig0006451:82886-82905 Msa1343540:three_prime_UTR 30.0%
AACAACAAACCAAAAACCAG+TGG + tig0006451:78534-78553 Msa1343540:five_prime_UTR 35.0%
ATGCTGATATTAACTCCGTT+TGG + tig0006451:78946-78965 Msa1343540:five_prime_UTR 35.0%
! GATTTGGTATGAAATCAGCT+AGG - tig0006451:78995-79014 None:intergenic 35.0%
! TCATACCAAATCTTGTTTGG+AGG + tig0006451:79003-79022 Msa1343540:five_prime_UTR 35.0%
ATGCCCTTATAATGAAGCTA+TGG - tig0006451:79052-79071 None:intergenic 35.0%
! CTTCATTATAAGGGCATGTT+TGG + tig0006451:79055-79074 Msa1343540:five_prime_UTR 35.0%
TAGAGCAAAGAGGATTTCAA+AGG + tig0006451:79398-79417 Msa1343540:CDS 35.0%
AGAGCAAAGAGGATTTCAAA+GGG + tig0006451:79399-79418 Msa1343540:CDS 35.0%
GATTTCAAAGGGCAATGTAT+TGG + tig0006451:79410-79429 Msa1343540:CDS 35.0%
AGAATGTGCTTACAGAATTG+AGG - tig0006451:79474-79493 None:intergenic 35.0%
CTATGGATAACATTGAAGAG+TGG + tig0006451:79552-79571 Msa1343540:intron 35.0%
! TGGAGACTTTAAGAAGTTTG+AGG + tig0006451:79757-79776 Msa1343540:CDS 35.0%
! GGAGACTTTAAGAAGTTTGA+GGG + tig0006451:79758-79777 Msa1343540:CDS 35.0%
GGAAAATGGTCTGTAAAATC+TGG + tig0006451:79779-79798 Msa1343540:CDS 35.0%
ATAGCTGGGAAATTGAATCT+TGG - tig0006451:79952-79971 None:intergenic 35.0%
!! CAATTTCCCAGCTATTTTCT+TGG + tig0006451:79957-79976 Msa1343540:CDS 35.0%
TCCTTTCCAAGAAAATAGCT+GGG - tig0006451:79966-79985 None:intergenic 35.0%
ATCCTTTCCAAGAAAATAGC+TGG - tig0006451:79967-79986 None:intergenic 35.0%
CCTTTCAACTCTATATGCTA+AGG - tig0006451:80022-80041 None:intergenic 35.0%
CCTTAGCATATAGAGTTGAA+AGG + tig0006451:80019-80038 Msa1343540:CDS 35.0%
! GAGTTGAAAGGAATCTTTTG+GGG + tig0006451:80031-80050 Msa1343540:CDS 35.0%
CGACTTGCATAAAACTTCTT+TGG + tig0006451:80080-80099 Msa1343540:CDS 35.0%
CAGTGAGTTAAACAGCAATT+GGG + tig0006451:80224-80243 Msa1343540:CDS 35.0%
AGTGAGTTAAACAGCAATTG+GGG + tig0006451:80225-80244 Msa1343540:CDS 35.0%
AACAGCAATTGGGGTATATT+TGG + tig0006451:80234-80253 Msa1343540:CDS 35.0%
TGAACTTCATCTACAACACA+AGG - tig0006451:80282-80301 None:intergenic 35.0%
TTTCATGTGAAGGAAAATGG+AGG + tig0006451:80409-80428 Msa1343540:intron 35.0%
GCATGCTGTAAAAGATTTCT+AGG + tig0006451:80565-80584 Msa1343540:intron 35.0%
!! ACTCTGGTTCATTTTCAGAT+TGG - tig0006451:80721-80740 None:intergenic 35.0%
AGATTCTCTTGTTCTAACTC+TGG - tig0006451:80737-80756 None:intergenic 35.0%
! TTTTATCGGCAATTGTGCTT+AGG - tig0006451:80857-80876 None:intergenic 35.0%
!!! ACAAGAGATCAGTTTTGAAC+AGG + tig0006451:80999-81018 Msa1343540:CDS 35.0%
!! AAATGGACTTTTGAGCAACT+AGG + tig0006451:81051-81070 Msa1343540:CDS 35.0%
GTGTCTTGCTTCAACTTATA+TGG - tig0006451:81238-81257 None:intergenic 35.0%
CTTATTGCACACAAGTTTGA+TGG - tig0006451:81440-81459 None:intergenic 35.0%
! CAAATTATATCATCTGGCTC+TGG + tig0006451:81530-81549 Msa1343540:CDS 35.0%
TAATCAAAGGTCAAGAGTTC+CGG + tig0006451:81613-81632 Msa1343540:CDS 35.0%
! ATTTGTTGCTGAATATGGTC+AGG + tig0006451:81679-81698 Msa1343540:CDS 35.0%
TTACAAATACCGCAAACCAA+AGG + tig0006451:81820-81839 Msa1343540:CDS 35.0%
TACAAATACCGCAAACCAAA+GGG + tig0006451:81821-81840 Msa1343540:CDS 35.0%
GGACAATCTTGAAAATTTGC+AGG + tig0006451:81850-81869 Msa1343540:CDS 35.0%
TGGCAAGTAGCAACTAAAAA+TGG - tig0006451:81900-81919 None:intergenic 35.0%
AATTGACGATGACATGATGA+AGG + tig0006451:81938-81957 Msa1343540:intron 35.0%
ACATGTGTAACATCGTTTAC+AGG + tig0006451:82003-82022 Msa1343540:intron 35.0%
TAAGTAGATTTCAGAGATGC+TGG + tig0006451:82060-82079 Msa1343540:CDS 35.0%
AAGTAGATTTCAGAGATGCT+GGG + tig0006451:82061-82080 Msa1343540:CDS 35.0%
TTCTACTGGGCATAAATGAT+GGG - tig0006451:82094-82113 None:intergenic 35.0%
TTTCTACTGGGCATAAATGA+TGG - tig0006451:82095-82114 None:intergenic 35.0%
CGTTCAAATGACTTTCTACT+GGG - tig0006451:82107-82126 None:intergenic 35.0%
TCGTTCAAATGACTTTCTAC+TGG - tig0006451:82108-82127 None:intergenic 35.0%
AGAAAGTCATTTGAACGAGT+TGG + tig0006451:82110-82129 Msa1343540:CDS 35.0%
AGTAAGATACATATGCCATG+TGG - tig0006451:82212-82231 None:intergenic 35.0%
! CATTTTTCTTATCCTTGCCA+AGG - tig0006451:82389-82408 None:intergenic 35.0%
!! TATCTACATCTGCTGATTCT+CGG - tig0006451:82416-82435 None:intergenic 35.0%
GCATACAGAACTAAAAGAGT+TGG + tig0006451:82488-82507 Msa1343540:CDS 35.0%
TAAGCAAGAATGTACAAGGA+AGG - tig0006451:82607-82626 None:intergenic 35.0%
CACCCTTCAATAGCAAAATA+TGG + tig0006451:82713-82732 Msa1343540:three_prime_UTR 35.0%
!!! CAGCCATATTTTGCTATTGA+AGG - tig0006451:82719-82738 None:intergenic 35.0%
TACTGATGCCATGTACTTAT+TGG + tig0006451:82763-82782 Msa1343540:three_prime_UTR 35.0%
GAGACAGACTATTTAATCCT+AGG + tig0006451:82822-82841 Msa1343540:three_prime_UTR 35.0%
GGACTTCAGTTCAAATTAAG+GGG + tig0006451:82843-82862 Msa1343540:three_prime_UTR 35.0%
AATTTCAGCTGTGGGATTAT+AGG - tig0006451:82961-82980 None:intergenic 35.0%
AGTCATACAATTTCAGCTGT+GGG - tig0006451:82969-82988 None:intergenic 35.0%
GATGATGTTTCATGGCATGT+TGG - tig0006451:78588-78607 None:intergenic 40.0%
TTTCCACCTAAATGGCTTCT+GGG - tig0006451:78813-78832 None:intergenic 40.0%
ATTTCCACCTAAATGGCTTC+TGG - tig0006451:78814-78833 None:intergenic 40.0%
CCTTACCTCCAAACAAGATT+TGG - tig0006451:79011-79030 None:intergenic 40.0%
! CCAAATCTTGTTTGGAGGTA+AGG + tig0006451:79008-79027 Msa1343540:five_prime_UTR 40.0%
ACCATATCCTTCCAGGAAAT+GGG - tig0006451:79378-79397 None:intergenic 40.0%
AACCATATCCTTCCAGGAAA+TGG - tig0006451:79379-79398 None:intergenic 40.0%
AGGATATGGTTAGAGCAAAG+AGG + tig0006451:79388-79407 Msa1343540:CDS 40.0%
!!! CATTGAAGCTCGTGTTGTTT+TGG + tig0006451:79435-79454 Msa1343540:CDS 40.0%
! GGTCTGTAAAATCTGGAACA+AGG + tig0006451:79786-79805 Msa1343540:CDS 40.0%
CCTGCAAAAAGCAGAATTGA+AGG - tig0006451:79890-79909 None:intergenic 40.0%
!!! CCTTCAATTCTGCTTTTTGC+AGG + tig0006451:79887-79906 Msa1343540:intron 40.0%
!! TCCCAGCTATTTTCTTGGAA+AGG + tig0006451:79962-79981 Msa1343540:CDS 40.0%
CTTGGAAAGGATTGTCAGAT+CGG + tig0006451:79975-79994 Msa1343540:CDS 40.0%
! GTTTTATGCAAGTCGTCTTC+TGG - tig0006451:80075-80094 None:intergenic 40.0%
AAACCTTCTTTATCCTGACC+AGG - tig0006451:80168-80187 None:intergenic 40.0%
TGGTCAGGATAAAGAAGGTT+TGG + tig0006451:80167-80186 Msa1343540:CDS 40.0%
GGTTTGGACACTTCAATTTC+CGG + tig0006451:80183-80202 Msa1343540:CDS 40.0%
!! CCAATTGCTGTTTAACTCAC+TGG - tig0006451:80226-80245 None:intergenic 40.0%
CCAGTGAGTTAAACAGCAAT+TGG + tig0006451:80223-80242 Msa1343540:CDS 40.0%
CCAAAAGTCCATCAAATCTG+CGG - tig0006451:80308-80327 None:intergenic 40.0%
ACATTCCATACATCACGAAC+AGG - tig0006451:80475-80494 None:intergenic 40.0%
GAAACACGCATTACTTACTC+AGG - tig0006451:80520-80539 None:intergenic 40.0%
CAATAAAGTCCGCATTCTTC+AGG + tig0006451:80689-80708 Msa1343540:CDS 40.0%
TGCTAGCAAGCATAATACAG+TGG - tig0006451:80785-80804 None:intergenic 40.0%
! AATATGCAGGAAGGGTGTAA+AGG + tig0006451:80919-80938 Msa1343540:intron 40.0%
GTGTAAAGGCCTGCTTTATA+TGG + tig0006451:80933-80952 Msa1343540:CDS 40.0%
! CATGAAGCACCATATAAAGC+AGG - tig0006451:80945-80964 None:intergenic 40.0%
!!! ATCAGTTTTGAACAGGCTGA+AGG + tig0006451:81006-81025 Msa1343540:CDS 40.0%
TCGACTCATTCCATGGAAAA+TGG + tig0006451:81034-81053 Msa1343540:CDS 40.0%
! GCTCAAAAGTCCATTTTCCA+TGG - tig0006451:81047-81066 None:intergenic 40.0%
!! GTTCCTTTCTGAAGCTATCA+TGG + tig0006451:81128-81147 Msa1343540:CDS 40.0%
CTTCCATGATAGCTTCAGAA+AGG - tig0006451:81134-81153 None:intergenic 40.0%
!! TTCTGAAGCTATCATGGAAG+AGG + tig0006451:81134-81153 Msa1343540:CDS 40.0%
TAAGAGACTACGTTGAGAAC+CGG + tig0006451:81456-81475 Msa1343540:CDS 40.0%
CAGGAAATTGTGATGCTTTC+CGG - tig0006451:81478-81497 None:intergenic 40.0%
GTATTCTGCTCACAAACTTC+AGG - tig0006451:81497-81516 None:intergenic 40.0%
AATCAAAGGTCAAGAGTTCC+GGG + tig0006451:81614-81633 Msa1343540:CDS 40.0%
TTGCTGAATATGGTCAGGAA+GGG + tig0006451:81684-81703 Msa1343540:CDS 40.0%
TGGAAGAGTGGATATTGAGA+AGG + tig0006451:81739-81758 Msa1343540:CDS 40.0%
AGCTAGAGAGAGGTTCATTA+TGG - tig0006451:81801-81820 None:intergenic 40.0%
GGTATTTGTAAGCTAGAGAG+AGG - tig0006451:81811-81830 None:intergenic 40.0%
TCTTGAAAATTTGCAGGACG+AGG + tig0006451:81856-81875 Msa1343540:CDS 40.0%
AGTAGATTTCAGAGATGCTG+GGG + tig0006451:82062-82081 Msa1343540:CDS 40.0%
!! AGTCATTTGAACGAGTTGGT+AGG + tig0006451:82114-82133 Msa1343540:intron 40.0%
GTTTGCGTTGCTTACTTTCA+AGG + tig0006451:82170-82189 Msa1343540:intron 40.0%
TATCGATGTAACATGCCACA+TGG + tig0006451:82194-82213 Msa1343540:intron 40.0%
TTGGGCTTAGCAGTAGATAA+TGG + tig0006451:82244-82263 Msa1343540:intron 40.0%
AAGGAGAAAAAGAACCAGAC+AGG + tig0006451:82356-82375 Msa1343540:CDS 40.0%
TTTCTCAAGACACACACAAG+TGG + tig0006451:82466-82485 Msa1343540:CDS 40.0%
GTGCTAAGCAAGAATGTACA+AGG - tig0006451:82611-82630 None:intergenic 40.0%
CAATAGCAAAATATGGCTGC+TGG + tig0006451:82720-82739 Msa1343540:three_prime_UTR 40.0%
TATTGCTATGACAGTTTGCG+TGG + tig0006451:82924-82943 Msa1343540:three_prime_UTR 40.0%
GAGTCATACAATTTCAGCTG+TGG - tig0006451:82970-82989 None:intergenic 40.0%
GTTTCATGGCATGTTGGCAT+AGG - tig0006451:78582-78601 None:intergenic 45.0%
TGAGTGTGAGTGTGTGAAGA+AGG - tig0006451:78632-78651 None:intergenic 45.0%
!! GGGTTAGCTCTTGAAGAAGA+AGG - tig0006451:78678-78697 None:intergenic 45.0%
! AGGAAGAGCGTAGAGAAAGT+GGG - tig0006451:78698-78717 None:intergenic 45.0%
! AAGGAAGAGCGTAGAGAAAG+TGG - tig0006451:78699-78718 None:intergenic 45.0%
TTTCCCTCGCAATTTGCACA+AGG + tig0006451:78755-78774 Msa1343540:five_prime_UTR 45.0%
TTCCCTCGCAATTTGCACAA+GGG + tig0006451:78756-78775 Msa1343540:five_prime_UTR 45.0%
TGCAAACCCAGAAGCCATTT+AGG + tig0006451:78804-78823 Msa1343540:five_prime_UTR 45.0%
AAACCCAGAAGCCATTTAGG+TGG + tig0006451:78807-78826 Msa1343540:five_prime_UTR 45.0%
AGAAGAAGAAAGTGACCGTG+AGG + tig0006451:78860-78879 Msa1343540:five_prime_UTR 45.0%
GAAGCTCACAGTTAACCTCA+CGG - tig0006451:78878-78897 None:intergenic 45.0%
GGTTAACTGTGAGCTTCAAG+TGG + tig0006451:78881-78900 Msa1343540:five_prime_UTR 45.0%
GTGAGCTTCAAGTGGTTTCT+TGG + tig0006451:78889-78908 Msa1343540:five_prime_UTR 45.0%
AGCTTCAAGTGGTTTCTTGG+AGG + tig0006451:78892-78911 Msa1343540:five_prime_UTR 45.0%
GCTTCAAGTGGTTTCTTGGA+GGG + tig0006451:78893-78912 Msa1343540:five_prime_UTR 45.0%
AAGTGGTTTCTTGGAGGGAA+CGG + tig0006451:78898-78917 Msa1343540:five_prime_UTR 45.0%
TGCTCTAACCATATCCTTCC+AGG - tig0006451:79385-79404 None:intergenic 45.0%
TTTAACTCACTGGAAGACGC+AGG - tig0006451:80216-80235 None:intergenic 45.0%
CTACAACACAAGGCTTGTCA+AGG - tig0006451:80272-80291 None:intergenic 45.0%
GTTCATCTCCGCAGATTTGA+TGG + tig0006451:80297-80316 Msa1343540:CDS 45.0%
!!! CCGCAGATTTGATGGACTTT+TGG + tig0006451:80305-80324 Msa1343540:CDS 45.0%
AAGCTCCTGTTCGTGATGTA+TGG + tig0006451:80467-80486 Msa1343540:CDS 45.0%
AAGTCCGCATTCTTCAGGTA+CGG + tig0006451:80694-80713 Msa1343540:intron 45.0%
AGCAAGCATAATACAGTGGC+AGG - tig0006451:80781-80800 None:intergenic 45.0%
! CACACTGCTAAAGTACTCTG+TGG + tig0006451:81083-81102 Msa1343540:CDS 45.0%
TCAATATCCCTCTGTAGTCC+CGG - tig0006451:81635-81654 None:intergenic 45.0%
GTTGCTGAATATGGTCAGGA+AGG + tig0006451:81683-81702 Msa1343540:CDS 45.0%
AGGAAGGGTTTATGCCTATG+AGG + tig0006451:81699-81718 Msa1343540:CDS 45.0%
TGAGAAGGCTATAACTCGCA+TGG + tig0006451:81754-81773 Msa1343540:CDS 45.0%
GAGAAGGCTATAACTCGCAT+GGG + tig0006451:81755-81774 Msa1343540:CDS 45.0%
AGATTGTCCCAGTAACCCTT+TGG - tig0006451:81839-81858 None:intergenic 45.0%
GACGATGACATGATGAAGGA+TGG + tig0006451:81942-81961 Msa1343540:intron 45.0%
GTAGATTTCAGAGATGCTGG+GGG + tig0006451:82063-82082 Msa1343540:CDS 45.0%
TTCTCGCCTTCTATCTCTCA+AGG + tig0006451:82332-82351 Msa1343540:CDS 45.0%
TCCTTGCCTTGAGAGATAGA+AGG - tig0006451:82341-82360 None:intergenic 45.0%
TAGCAAAATATGGCTGCTGG+TGG + tig0006451:82723-82742 Msa1343540:three_prime_UTR 45.0%
AGCAAAATATGGCTGCTGGT+GGG + tig0006451:82724-82743 Msa1343540:three_prime_UTR 45.0%
! TCCTTTCACTTGGCGCATTT+TGG + tig0006451:82796-82815 Msa1343540:three_prime_UTR 45.0%
TCCAAAATGCGCCAAGTGAA+AGG - tig0006451:82800-82819 None:intergenic 45.0%
!!! ATAAATATATTTATACATTT+TGG + tig0006451:82544-82563 Msa1343540:three_prime_UTR 5.0%
!!! GCATTGTGGCCACTGGTTTT+TGG - tig0006451:78546-78565 None:intergenic 50.0%
TGCCCTTGTGCAAATTGCGA+GGG - tig0006451:78761-78780 None:intergenic 50.0%
ATGCCCTTGTGCAAATTGCG+AGG - tig0006451:78762-78781 None:intergenic 50.0%
! GGGTTTGCACTGTGTTGTTG+AGG - tig0006451:78793-78812 None:intergenic 50.0%
TTGGAGGGAACGGAGAGTTA+AGG + tig0006451:78908-78927 Msa1343540:five_prime_UTR 50.0%
CGTATTCCTTGCCCATTTCC+TGG + tig0006451:79364-79383 Msa1343540:CDS 50.0%
TTCCTTGCCCATTTCCTGGA+AGG + tig0006451:79368-79387 Msa1343540:CDS 50.0%
ATCCTTCCAGGAAATGGGCA+AGG - tig0006451:79373-79392 None:intergenic 50.0%
GCCCATTTCCTGGAAGGATA+TGG + tig0006451:79374-79393 Msa1343540:CDS 50.0%
GCTAAGGCTCGTAGATTCAC+AGG - tig0006451:80006-80025 None:intergenic 50.0%
! ATGCAAGTCGTCTTCTGGCT+GGG - tig0006451:80070-80089 None:intergenic 50.0%
TATGCAAGTCGTCTTCTGGC+TGG - tig0006451:80071-80090 None:intergenic 50.0%
GAAACCGACAAATTGGCACC+TGG + tig0006451:80147-80166 Msa1343540:CDS 50.0%
CTGACCAGGTGCCAATTTGT+CGG - tig0006451:80154-80173 None:intergenic 50.0%
GCACCTGGTCAGGATAAAGA+AGG + tig0006451:80162-80181 Msa1343540:CDS 50.0%
TGGTCCGTACCTGAAGAATG+CGG - tig0006451:80701-80720 None:intergenic 50.0%
TATGGTGCTTCATGCTCGTG+TGG + tig0006451:80951-80970 Msa1343540:CDS 50.0%
GGTGACTTCGACTCATTCCA+TGG + tig0006451:81027-81046 Msa1343540:CDS 50.0%
AAGAGTTCCGGGACTACAGA+GGG + tig0006451:81625-81644 Msa1343540:CDS 50.0%
TGCCTATGAGGAAGCAGCTT+CGG + tig0006451:81711-81730 Msa1343540:CDS 50.0%
AAGGCTATAACTCGCATGGG+TGG + tig0006451:81758-81777 Msa1343540:CDS 50.0%
ACCGCAAACCAAAGGGTTAC+TGG + tig0006451:81828-81847 Msa1343540:CDS 50.0%
!! CCCAGTAACCCTTTGGTTTG+CGG - tig0006451:81832-81851 None:intergenic 50.0%
CCGCAAACCAAAGGGTTACT+GGG + tig0006451:81829-81848 Msa1343540:CDS 50.0%
ATTTCAGAGATGCTGGGGGA+TGG + tig0006451:82067-82086 Msa1343540:CDS 50.0%
! CAGGCCGCTTTGATATTGCA+CGG + tig0006451:82277-82296 Msa1343540:intron 50.0%
! AGGCCGCTTTGATATTGCAC+GGG + tig0006451:82278-82297 Msa1343540:intron 50.0%
! AAAGTGGGGTGGACTTCATG+AGG + tig0006451:82308-82327 Msa1343540:CDS 50.0%
GCCTTCTATCTCTCAAGGCA+AGG + tig0006451:82337-82356 Msa1343540:CDS 50.0%
ACCAGACAGGACAACAACCT+TGG + tig0006451:82369-82388 Msa1343540:CDS 50.0%
ACAGGACAACAACCTTGGCA+AGG + tig0006451:82374-82393 Msa1343540:CDS 50.0%
! GCTACAGAGTGCGAGCATTG+TGG - tig0006451:78560-78579 None:intergenic 55.0%
! TGCAAGTCGTCTTCTGGCTG+GGG - tig0006451:80069-80088 None:intergenic 55.0%
CGACAAATTGGCACCTGGTC+AGG + tig0006451:80152-80171 Msa1343540:CDS 55.0%
GGAAGACGCAGGCAATGAAC+CGG - tig0006451:80205-80224 None:intergenic 55.0%
CAAGAGTTCCGGGACTACAG+AGG + tig0006451:81624-81643 Msa1343540:CDS 55.0%
AGCCGAAGCTGCTTCCTCAT+AGG - tig0006451:81716-81735 None:intergenic 55.0%
GCGCCCGTGCAATATCAAAG+CGG - tig0006451:82284-82303 None:intergenic 55.0%
CTTTGATATTGCACGGGCGC+TGG + tig0006451:82284-82303 Msa1343540:CDS 55.0%
GCCAAGGTTGTTGTCCTGTC+TGG - tig0006451:82373-82392 None:intergenic 55.0%
! AGTGCGAGCATTGTGGCCAC+TGG - tig0006451:78553-78572 None:intergenic 60.0%
AGGAAGCAGCTTCGGCTGCA+TGG + tig0006451:81719-81738 Msa1343540:CDS 60.0%
GCTTCGGCTGCATGGAAGAG+TGG + tig0006451:81727-81746 Msa1343540:CDS 60.0%
!! TTGCACGGGCGCTGGAAAAG+TGG + tig0006451:82292-82311 Msa1343540:CDS 60.0%
!! TGCACGGGCGCTGGAAAAGT+GGG + tig0006451:82293-82312 Msa1343540:CDS 60.0%
!! GCACGGGCGCTGGAAAAGTG+GGG + tig0006451:82294-82313 Msa1343540:CDS 65.0%
!! CGGGCGCTGGAAAAGTGGGG+TGG + tig0006451:82297-82316 Msa1343540:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0006451 gene 78531 82996 78531 ID=Msa1343540;Name=Msa1343540
tig0006451 mRNA 78531 82996 78531 ID=Msa1343540-mRNA-1;Parent=Msa1343540;Name=Msa1343540-mRNA-1;_AED=0.04;_eAED=0.04;_QI=788|1|1|1|1|1|9|466|614
tig0006451 exon 78531 79480 78531 ID=Msa1343540-mRNA-1:exon:4164;Parent=Msa1343540-mRNA-1
tig0006451 exon 79722 79807 79722 ID=Msa1343540-mRNA-1:exon:4165;Parent=Msa1343540-mRNA-1
tig0006451 exon 79909 80326 79909 ID=Msa1343540-mRNA-1:exon:4166;Parent=Msa1343540-mRNA-1
tig0006451 exon 80421 80521 80421 ID=Msa1343540-mRNA-1:exon:4167;Parent=Msa1343540-mRNA-1
tig0006451 exon 80644 80710 80644 ID=Msa1343540-mRNA-1:exon:4168;Parent=Msa1343540-mRNA-1
tig0006451 exon 80928 81155 80928 ID=Msa1343540-mRNA-1:exon:4169;Parent=Msa1343540-mRNA-1
tig0006451 exon 81419 81877 81419 ID=Msa1343540-mRNA-1:exon:4170;Parent=Msa1343540-mRNA-1
tig0006451 exon 82059 82131 82059 ID=Msa1343540-mRNA-1:exon:4171;Parent=Msa1343540-mRNA-1
tig0006451 exon 82280 82996 82280 ID=Msa1343540-mRNA-1:exon:4172;Parent=Msa1343540-mRNA-1
tig0006451 five_prime_UTR 78531 79318 78531 ID=Msa1343540-mRNA-1:five_prime_utr;Parent=Msa1343540-mRNA-1
tig0006451 CDS 79319 79480 79319 ID=Msa1343540-mRNA-1:cds;Parent=Msa1343540-mRNA-1
tig0006451 CDS 79722 79807 79722 ID=Msa1343540-mRNA-1:cds;Parent=Msa1343540-mRNA-1
tig0006451 CDS 79909 80326 79909 ID=Msa1343540-mRNA-1:cds;Parent=Msa1343540-mRNA-1
tig0006451 CDS 80421 80521 80421 ID=Msa1343540-mRNA-1:cds;Parent=Msa1343540-mRNA-1
tig0006451 CDS 80644 80710 80644 ID=Msa1343540-mRNA-1:cds;Parent=Msa1343540-mRNA-1
tig0006451 CDS 80928 81155 80928 ID=Msa1343540-mRNA-1:cds;Parent=Msa1343540-mRNA-1
tig0006451 CDS 81419 81877 81419 ID=Msa1343540-mRNA-1:cds;Parent=Msa1343540-mRNA-1
tig0006451 CDS 82059 82131 82059 ID=Msa1343540-mRNA-1:cds;Parent=Msa1343540-mRNA-1
tig0006451 CDS 82280 82530 82280 ID=Msa1343540-mRNA-1:cds;Parent=Msa1343540-mRNA-1
tig0006451 three_prime_UTR 82531 82996 82531 ID=Msa1343540-mRNA-1:three_prime_utr;Parent=Msa1343540-mRNA-1
Gene Sequence

>Msa1343540

ATGCAATATGTGAATAATATGAGAGTTGTTTTTGTTGTTAGTGGGCGTATTCCTTGCCCATTTCCTGGAAGGATATGGTTAGAGCAAAGAGGATTTCAAAGGGCAATGTATTGGCACATTGAAGCTCGTGTTGTTTTGGATCTTCAAGAATTCCTCAATTCTGAATGGGATCGAGAACTTCATTTTTCAATGGTTGATGGAGACTTTAAGAAGTTTGAGGGAAAATGGTCTGTAAAATCTGGAACAAGGTCATCATCAACTAATTTATCTTATGAGGTTAATGTGATACCAAGATTCAATTTCCCAGCTATTTTCTTGGAAAGGATTGTCAGATCGGATCTTCCTGTGAATCTACGAGCCTTAGCATATAGAGTTGAAAGGAATCTTTTGGGGAATCAGAAATTTCCCCAGCCAGAAGACGACTTGCATAAAACTTCTTTGGTTGTTAATGGATCTTCTGTCAAAAAGATAAATGGTTCTTTATGTGAAACCGACAAATTGGCACCTGGTCAGGATAAAGAAGGTTTGGACACTTCAATTTCCGGTTCATTGCCTGCGTCTTCCAGTGAGTTAAACAGCAATTGGGGTATATTTGGAAAAGTTTGTAGCCTTGACAAGCCTTGTGTTGTAGATGAAGTTCATCTCCGCAGATTTGATGGACTTTTGGAAAATGGAGGTGTTCATCGTTGTGTCGTTGCAAGCATAACAGTTAAAGCTCCTGTTCGTGATGTATGGAATGTTATGTCTTCGTATGAGACTCTTCCTGAGATAGTTCCAAATTTAGCAATCAGTAAGATTTTATCACGAGATAACAATAAAGTCCGCATTCTTCAGGAAGGGTGTAAAGGCCTGCTTTATATGGTGCTTCATGCTCGTGTGGTGTTAGATTTGTGTGAACAGCTAGAACAAGAGATCAGTTTTGAACAGGCTGAAGGTGACTTCGACTCATTCCATGGAAAATGGACTTTTGAGCAACTAGGAAATCATCACACACTGCTAAAGTACTCTGTGGACTCGAAAATGCGCAGAGATACGTTCCTTTCTGAAGCTATCATGGAAGAGGTCATTTATGAAGATCTGCCATCAAACTTGTGTGCAATAAGAGACTACGTTGAGAACCGGAAAGCATCACAATTTCCTGAAGTTTGTGAGCAGAATACGAATTCAGAACAACAAATTATATCATCTGGCTCTGGAGATGATAGCAACTCTAGTTCAGCTGATGATTTTTCTGATTGTAATGTTCAAAGCTCATCTAATCAAAGGTCAAGAGTTCCGGGACTACAGAGGGATATTGAAGTCCTAAAATCTGAACTCCTGAAATTTGTTGCTGAATATGGTCAGGAAGGGTTTATGCCTATGAGGAAGCAGCTTCGGCTGCATGGAAGAGTGGATATTGAGAAGGCTATAACTCGCATGGGTGGATTCAGAAAGATTGCAACCATAATGAACCTCTCTCTAGCTTACAAATACCGCAAACCAAAGGGTTACTGGGACAATCTTGAAAATTTGCAGGACGAGATAAGTAGATTTCAGAGATGCTGGGGGATGGACCCATCATTTATGCCCAGTAGAAAGTCATTTGAACGAGTTGGCCGCTTTGATATTGCACGGGCGCTGGAAAAGTGGGGTGGACTTCATGAGGTTTCTCGCCTTCTATCTCTCAAGGCAAGGAGAAAAAGAACCAGACAGGACAACAACCTTGGCAAGGATAAGAAAAATGATCACCGAGAATCAGCAGATGTAGATAGTGAGATCAATGCAGCATCTAGACCTTATATTTCTCAAGACACACACAAGTGGCATACAGAACTAAAAGAGTTGGACATAAATTGGGTTGAGTAA
Protein sequence

>Msa1343540

MQYVNNMRVVFVVSGRIPCPFPGRIWLEQRGFQRAMYWHIEARVVLDLQEFLNSEWDRELHFSMVDGDFKKFEGKWSVKSGTRSSSTNLSYEVNVIPRFNFPAIFLERIVRSDLPVNLRALAYRVERNLLGNQKFPQPEDDLHKTSLVVNGSSVKKINGSLCETDKLAPGQDKEGLDTSISGSLPASSSELNSNWGIFGKVCSLDKPCVVDEVHLRRFDGLLENGGVHRCVVASITVKAPVRDVWNVMSSYETLPEIVPNLAISKILSRDNNKVRILQEGCKGLLYMVLHARVVLDLCEQLEQEISFEQAEGDFDSFHGKWTFEQLGNHHTLLKYSVDSKMRRDTFLSEAIMEEVIYEDLPSNLCAIRDYVENRKASQFPEVCEQNTNSEQQIISSGSGDDSNSSSADDFSDCNVQSSSNQRSRVPGLQRDIEVLKSELLKFVAEYGQEGFMPMRKQLRLHGRVDIEKAITRMGGFRKIATIMNLSLAYKYRKPKGYWDNLENLQDEISRFQRCWGMDPSFMPSRKSFERVGRFDIARALEKWGGLHEVSRLLSLKARRKRTRQDNNLGKDKKNDHRESADVDSEINAASRPYISQDTHKWHTELKELDINWVE*