AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1449910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449910 MtrunA17_Chr1g0210761 97.619 210 5 0 1 210 1 210 1.78e-150 416
Msa1449910 MtrunA17_Chr1g0178711 57.353 204 76 1 17 209 20 223 3.20e-83 246
Msa1449910 MtrunA17_Chr1g0193671 42.381 210 114 3 7 209 7 216 1.40e-53 171
Msa1449910 MtrunA17_Chr7g0271211 44.898 196 99 4 22 209 19 213 3.13e-52 167
Msa1449910 MtrunA17_Chr1g0193421 43.434 198 105 3 19 209 20 217 2.92e-51 165
Msa1449910 MtrunA17_Chr6g0449751 44.186 215 104 6 7 207 3 215 1.59e-48 158
Msa1449910 MtrunA17_Chr6g0449731 44.444 216 105 6 5 207 2 215 3.82e-48 157
Msa1449910 MtrunA17_Chr6g0449761 44.186 215 107 5 5 207 2 215 7.78e-48 157
Msa1449910 MtrunA17_Chr6g0449771 44.444 216 105 6 5 207 2 215 1.12e-47 155
Msa1449910 MtrunA17_Chr1g0189841 44.554 202 97 6 19 207 18 217 1.16e-47 155
Msa1449910 MtrunA17_Chr4g0007051 42.512 207 91 7 19 206 18 215 3.03e-45 149
Msa1449910 MtrunA17_Chr4g0007031 42.584 209 88 7 19 206 18 215 3.10e-45 149
Msa1449910 MtrunA17_Chr5g0419971 42.584 209 88 7 19 206 18 215 3.10e-45 149
Msa1449910 MtrunA17_Chr5g0419981 42.584 209 88 7 19 206 18 215 3.10e-45 149
Msa1449910 MtrunA17_Chr4g0007041 41.071 224 95 8 9 206 3 215 1.01e-44 148
Msa1449910 MtrunA17_Chr2g0285871 42.584 209 88 7 19 206 18 215 1.84e-44 147
Msa1449910 MtrunA17_Chr1g0156131 40.099 202 114 4 11 207 10 209 8.17e-44 145
Msa1449910 MtrunA17_Chr5g0414971 42.268 194 104 4 22 207 26 219 2.37e-43 145
Msa1449910 MtrunA17_Chr2g0292831 41.538 195 106 5 20 207 19 212 4.97e-43 144
Msa1449910 MtrunA17_Chr2g0292901 42.021 188 101 5 27 207 27 213 8.87e-43 143
Msa1449910 MtrunA17_Chr2g0300581 37.255 204 119 3 9 205 3 204 1.41e-38 132
Msa1449910 MtrunA17_Chr2g0300591 37.438 203 118 3 9 204 3 203 1.59e-38 132
Msa1449910 MtrunA17_Chr6g0449781 40.196 204 100 6 19 207 18 214 2.58e-38 132
Msa1449910 MtrunA17_Chr2g0292861 42.697 178 84 5 42 207 7 178 4.12e-38 130
Msa1449910 MtrunA17_Chr2g0300561 37.313 201 121 2 9 204 3 203 1.14e-37 130
Msa1449910 MtrunA17_Chr2g0292841 42.442 172 93 4 42 207 7 178 1.35e-36 126
Msa1449910 MtrunA17_Chr8g0343911 36.538 208 114 5 10 205 1 202 3.29e-36 126
Msa1449910 MtrunA17_Chr2g0313961 34.783 207 124 5 8 207 3 205 6.31e-35 122
Msa1449910 MtrunA17_Chr8g0344041 33.014 209 131 5 5 207 4 209 1.99e-34 121
Msa1449910 MtrunA17_Chr3g0084531 34.300 207 125 5 8 207 3 205 3.37e-34 120
Msa1449910 MtrunA17_Chr2g0297231 33.981 206 127 4 8 207 3 205 4.21e-34 120
Msa1449910 MtrunA17_Chr8g0343921 32.850 207 128 5 8 207 1 203 7.24e-34 120
Msa1449910 MtrunA17_Chr8g0343934 35.437 206 124 6 8 207 3 205 8.87e-34 120
Msa1449910 MtrunA17_Chr2g0321601 34.286 210 112 5 12 208 8 204 9.79e-34 119
Msa1449910 MtrunA17_Chr6g0449791 39.109 202 108 5 7 197 9 206 2.39e-33 119
Msa1449910 MtrunA17_Chr3g0130241 41.176 170 88 2 15 172 75 244 4.16e-33 120
Msa1449910 MtrunA17_Chr2g0297241 33.495 206 128 4 8 207 3 205 8.53e-33 117
Msa1449910 MtrunA17_Chr3g0084521 35.266 207 119 6 8 207 6 204 2.23e-32 116
Msa1449910 MtrunA17_Chr2g0302171 36.517 178 106 4 33 207 32 205 2.78e-32 116
Msa1449910 MtrunA17_Chr8g0343954 37.433 187 108 6 27 207 22 205 4.54e-32 115
Msa1449910 MtrunA17_Chr8g0335851 34.896 192 117 2 17 200 20 211 6.06e-32 115
Msa1449910 MtrunA17_Chr3g0084511 35.749 207 118 7 8 207 6 204 1.02e-31 114
Msa1449910 MtrunA17_Chr2g0301611 32.850 207 128 5 8 207 3 205 3.83e-31 113
Msa1449910 MtrunA17_Chr4g0003361 33.163 196 122 4 16 207 11 201 5.37e-31 112
Msa1449910 MtrunA17_Chr8g0343961 33.990 203 125 5 11 207 6 205 9.41e-31 112
Msa1449910 MtrunA17_Chr4g0026191 33.019 212 122 5 5 208 7 206 1.54e-30 111
Msa1449910 MtrunA17_Chr2g0321581 31.731 208 120 5 12 208 9 205 5.10e-30 110
Msa1449910 MtrunA17_Chr2g0321591 31.731 208 120 5 12 208 9 205 5.49e-30 110
Msa1449910 MtrunA17_Chr8g0344021 33.173 208 128 4 8 207 4 208 1.01e-29 109
Msa1449910 MtrunA17_Chr4g0026201 37.500 160 96 2 53 208 47 206 1.05e-29 109
Msa1449910 MtrunA17_Chr2g0302541 34.043 188 113 5 27 207 22 205 6.07e-28 104
Msa1449910 MtrunA17_Chr3g0115141 34.595 185 110 5 29 207 27 206 7.54e-28 104
Msa1449910 MtrunA17_Chr4g0012651 32.979 188 115 4 27 207 22 205 3.20e-27 103
Msa1449910 MtrunA17_Chr7g0217811 31.351 185 122 2 27 207 22 205 5.80e-27 102
Msa1449910 MtrunA17_Chr5g0411961 30.097 206 135 4 8 207 3 205 1.10e-26 101
Msa1449910 MtrunA17_Chr8g0344051 30.097 206 137 3 8 207 4 208 1.23e-26 101
Msa1449910 MtrunA17_Chr2g0292911 45.299 117 61 2 42 155 7 123 1.24e-26 99.4
Msa1449910 MtrunA17_Chr8g0344031 32.381 210 126 8 8 207 4 207 2.45e-26 100
Msa1449910 MtrunA17_Chr2g0286171 31.720 186 114 7 29 207 29 208 6.46e-24 94.4
Msa1449910 MtrunA17_Chr3g0084551 29.064 203 129 5 8 204 3 196 5.87e-21 87.0
Msa1449910 MtrunA17_Chr3g0084501 34.906 106 59 4 8 106 3 105 4.19e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449910 AT3G10080.1 60.194 206 70 3 13 209 19 221 2.19e-86 254
Msa1449910 AT3G10080.2 60.194 206 70 3 13 209 90 292 8.00e-86 256
Msa1449910 AT1G02335.1 42.791 215 115 4 1 207 1 215 2.84e-56 178
Msa1449910 AT3G04200.1 45.540 213 103 5 8 209 14 224 2.01e-54 173
Msa1449910 AT1G18980.1 45.192 208 105 4 7 207 13 218 8.80e-53 169
Msa1449910 AT1G18970.1 42.788 208 110 4 7 207 13 218 4.44e-52 167
Msa1449910 AT5G26700.1 40.865 208 116 4 7 209 6 211 5.87e-52 167
Msa1449910 AT5G39160.3 41.346 208 112 5 7 207 11 215 5.19e-51 164
Msa1449910 AT3G62020.1 43.204 206 109 4 12 209 12 217 5.27e-51 164
Msa1449910 AT5G39160.1 40.845 213 110 6 7 207 11 219 6.23e-51 164
Msa1449910 AT3G05950.1 44.393 214 104 6 7 208 12 222 9.50e-51 164
Msa1449910 AT5G39130.1 40.376 213 111 6 7 207 11 219 1.03e-50 164
Msa1449910 AT5G39190.1 40.845 213 110 6 7 207 11 219 1.13e-50 164
Msa1449910 AT5G38960.1 44.059 202 99 5 19 207 22 222 3.19e-50 162
Msa1449910 AT1G09560.1 44.103 195 102 3 22 209 23 217 5.69e-50 162
Msa1449910 AT3G05930.1 38.710 217 125 3 1 209 1 217 6.32e-49 159
Msa1449910 AT5G39110.1 41.014 217 113 6 5 207 5 220 2.70e-48 157
Msa1449910 AT5G38940.1 42.424 198 101 5 22 207 25 221 1.04e-47 156
Msa1449910 AT5G38940.2 42.424 198 101 5 22 207 49 245 1.55e-47 156
Msa1449910 AT5G39150.1 40.183 219 105 6 8 207 8 219 1.58e-47 155
Msa1449910 AT5G39180.1 40.183 219 105 6 8 207 8 219 2.42e-47 155
Msa1449910 AT5G38910.1 41.860 215 110 6 6 207 7 219 2.71e-47 155
Msa1449910 AT3G62020.2 45.699 186 95 4 28 209 5 188 3.30e-47 154
Msa1449910 AT5G39120.1 40.000 225 106 7 5 207 2 219 4.48e-47 154
Msa1449910 AT5G39160.2 39.130 207 100 6 7 207 11 197 1.65e-45 150
Msa1449910 AT3G04180.1 40.000 200 109 4 20 208 23 222 2.30e-45 150
Msa1449910 AT5G39190.2 39.130 207 100 6 7 207 11 197 2.75e-45 149
Msa1449910 AT3G04170.1 41.784 213 110 5 8 210 13 221 2.95e-45 150
Msa1449910 AT3G04150.1 40.094 212 114 5 8 208 13 222 7.33e-45 149
Msa1449910 AT4G14630.1 39.500 200 107 5 21 207 24 222 1.77e-44 148
Msa1449910 AT3G04190.1 40.796 201 106 5 20 208 23 222 4.90e-44 147
Msa1449910 AT5G38930.1 40.201 199 106 5 21 207 24 221 1.36e-43 145
Msa1449910 AT3G04150.2 38.667 225 112 6 8 208 13 235 3.18e-43 145
Msa1449910 AT5G20630.1 39.344 183 106 2 27 204 22 204 3.40e-40 137
Msa1449910 AT1G10460.1 37.186 199 111 5 22 207 18 215 2.09e-39 135
Msa1449910 AT5G61750.1 39.785 186 92 5 9 181 5 183 1.51e-34 122
Msa1449910 AT1G72610.1 37.838 185 106 3 27 204 19 201 1.23e-33 120
Msa1449910 AT1G74820.1 31.390 223 134 6 2 208 7 226 7.63e-33 118
Msa1449910 AT5G38910.2 35.211 213 90 6 6 207 7 182 1.55e-30 111
Msa1449910 AT5G38950.1 49.206 63 30 1 138 198 2 64 2.53e-12 61.6

Find 21 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGATTAAAGATGAGCTTT+TGG 0.232327 tig0032745:-8659 Msa1449910:CDS
GAATTTGTTATCTTCTGATT+TGG 0.267937 tig0032745:+9107 None:intergenic
CCAGTTTCTTTGAAGTTTCC+TGG 0.322734 tig0032745:+9024 None:intergenic
TTCTGATCCGGATCCTGTTA+TGG 0.323739 tig0032745:-9142 Msa1449910:CDS
CCAGGAAACTTCAAAGAAAC+TGG 0.339479 tig0032745:-9024 Msa1449910:CDS
GTGAATTCAAATGTGTTTCC+AGG 0.358885 tig0032745:-8985 Msa1449910:CDS
TTTGGAGAAAGGTGAGGTTA+TGG 0.393881 tig0032745:-8803 Msa1449910:CDS
TGAAAATGCACAAGTCCTCT+TGG 0.414763 tig0032745:+8775 None:intergenic
GCTATGCAGTAATCCATAAC+AGG 0.434646 tig0032745:+9129 None:intergenic
ATAGCAACTTCTGTTGCTCT+TGG 0.444865 tig0032745:+8889 None:intergenic
CAGTAATCCATAACAGGATC+CGG 0.469062 tig0032745:+9135 None:intergenic
TTTCCAGGTTTAAACACTCT+TGG 0.469660 tig0032745:-8970 Msa1449910:CDS
CAGCAATGATGAAACACATA+AGG 0.504374 tig0032745:+9172 None:intergenic
ACACATAAGGACTAAAAGAA+TGG 0.521199 tig0032745:+9185 None:intergenic
AGAAGTTGCTATTGTGTTGG+AGG 0.523101 tig0032745:-8878 Msa1449910:CDS
AACAGAAGTTGCTATTGTGT+TGG 0.541553 tig0032745:-8881 Msa1449910:CDS
GAAGTTGCTATTGTGTTGGA+GGG 0.567803 tig0032745:-8877 Msa1449910:CDS
ACTCCAAGAGTGTTTAAACC+TGG 0.567991 tig0032745:+8967 None:intergenic
GAAATCTTCAACTGTTGCTG+TGG 0.571728 tig0032745:+9068 None:intergenic
AGCTTTCTAAGTTAAAGAAG+AGG 0.576087 tig0032745:-8609 Msa1449910:CDS
GTTGCTCTTGGATGAAAGTG+AGG 0.618955 tig0032745:+8901 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATTTGTTATCTTCTGATT+TGG + tig0032745:8694-8713 None:intergenic 25.0%
!! CAAGATTTTTGCTAAAGTTT+TGG - tig0032745:8977-8996 Msa1449910:CDS 25.0%
!!! AAAATTCCAAATGCTGTTTT+TGG - tig0032745:9113-9132 Msa1449910:CDS 25.0%
ACACATAAGGACTAAAAGAA+TGG + tig0032745:8616-8635 None:intergenic 30.0%
!!! GTTGAAGATTTCACTTTTTC+TGG - tig0032745:8741-8760 Msa1449910:CDS 30.0%
!! TTTTCTGGTATAAAACTACC+AGG - tig0032745:8756-8775 Msa1449910:CDS 30.0%
! AGAAACTGGATTTTCTTCAA+TGG - tig0032745:8788-8807 Msa1449910:CDS 30.0%
! GAAACTGGATTTTCTTCAAT+GGG - tig0032745:8789-8808 Msa1449910:CDS 30.0%
TTGGAGGGAAAAATTTATTC+AGG - tig0032745:8936-8955 Msa1449910:CDS 30.0%
!!! TTTGCTAAAGTTTTGGAGAA+AGG - tig0032745:8984-9003 Msa1449910:CDS 30.0%
AAACTACCAAAAATAGTAGC+AGG + tig0032745:9068-9087 None:intergenic 30.0%
!! AGTTTTGATAGTCAAAATCC+TGG - tig0032745:9083-9102 Msa1449910:CDS 30.0%
!! TTTGGAATTTTCATCAAACC+AGG + tig0032745:9104-9123 None:intergenic 30.0%
! TGAGATTAAAGATGAGCTTT+TGG - tig0032745:9139-9158 Msa1449910:CDS 30.0%
! AGCTTTTGGACTTAATTCTA+AGG - tig0032745:9166-9185 Msa1449910:CDS 30.0%
AGCTTTCTAAGTTAAAGAAG+AGG - tig0032745:9189-9208 Msa1449910:CDS 30.0%
CAGCAATGATGAAACACATA+AGG + tig0032745:8629-8648 None:intergenic 35.0%
GTGAATTCAAATGTGTTTCC+AGG - tig0032745:8813-8832 Msa1449910:CDS 35.0%
TTTCCAGGTTTAAACACTCT+TGG - tig0032745:8828-8847 Msa1449910:CDS 35.0%
!! GTTAGAGCAGATTTTGATGT+TGG - tig0032745:8861-8880 Msa1449910:CDS 35.0%
! AACAGAAGTTGCTATTGTGT+TGG - tig0032745:8917-8936 Msa1449910:CDS 35.0%
!!! TAAAGTTTTGGAGAAAGGTG+AGG - tig0032745:8989-9008 Msa1449910:CDS 35.0%
! GTGCATTTTCAGATGAATGT+TGG - tig0032745:9035-9054 Msa1449910:CDS 35.0%
! GATGTTCCTGCTACTATTTT+TGG - tig0032745:9059-9078 Msa1449910:CDS 35.0%
TCAGATCCAAAAACAGCATT+TGG + tig0032745:9122-9141 None:intergenic 35.0%
!! GAGCTTTTGGAAAAAGCTTT+TGG - tig0032745:9152-9171 Msa1449910:CDS 35.0%
!!! TGCTGCTTTTTGTTCTGATC+CGG - tig0032745:8644-8663 Msa1449910:CDS 40.0%
CAGTAATCCATAACAGGATC+CGG + tig0032745:8666-8685 None:intergenic 40.0%
GCTATGCAGTAATCCATAAC+AGG + tig0032745:8672-8691 None:intergenic 40.0%
GAAATCTTCAACTGTTGCTG+TGG + tig0032745:8733-8752 None:intergenic 40.0%
!! CCAGTTTCTTTGAAGTTTCC+TGG + tig0032745:8777-8796 None:intergenic 40.0%
CCAGGAAACTTCAAAGAAAC+TGG - tig0032745:8774-8793 Msa1449910:CDS 40.0%
!! ACTCCAAGAGTGTTTAAACC+TGG + tig0032745:8834-8853 None:intergenic 40.0%
!! AGAGCAGATTTTGATGTTGG+TGG - tig0032745:8864-8883 Msa1449910:CDS 40.0%
ATAGCAACTTCTGTTGCTCT+TGG + tig0032745:8912-8931 None:intergenic 40.0%
! AGAAGTTGCTATTGTGTTGG+AGG - tig0032745:8920-8939 Msa1449910:CDS 40.0%
! GAAGTTGCTATTGTGTTGGA+GGG - tig0032745:8921-8940 Msa1449910:CDS 40.0%
TTTGGAGAAAGGTGAGGTTA+TGG - tig0032745:8995-9014 Msa1449910:CDS 40.0%
! GAGGTTATGGTTTTTCCAAG+AGG - tig0032745:9008-9027 Msa1449910:CDS 40.0%
TGAAAATGCACAAGTCCTCT+TGG + tig0032745:9026-9045 None:intergenic 40.0%
TTCTGATCCGGATCCTGTTA+TGG - tig0032745:8656-8675 Msa1449910:CDS 45.0%
GTTGCTCTTGGATGAAAGTG+AGG + tig0032745:8900-8919 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
tig0032745 gene 8594 9226 8594 ID=Msa1449910;Name=Msa1449910
tig0032745 mRNA 8594 9226 8594 ID=Msa1449910-mRNA-1;Parent=Msa1449910;Name=Msa1449910-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|0|210
tig0032745 exon 8594 9226 8594 ID=Msa1449910-mRNA-1:exon:17187;Parent=Msa1449910-mRNA-1
tig0032745 CDS 8594 9226 8594 ID=Msa1449910-mRNA-1:cds;Parent=Msa1449910-mRNA-1
Gene Sequence

>Msa1449910

ATGATCAACAACAAAGTAACCATTCTTTTAGTCCTTATGTGTTTCATCATTGCTGCTTTTTGTTCTGATCCGGATCCTGTTATGGATTACTGCATAGCCAAATCAGAAGATAACAAATTCAGTTGCAAAAACTCATCCACAGCAACAGTTGAAGATTTCACTTTTTCTGGTATAAAACTACCAGGAAACTTCAAAGAAACTGGATTTTCTTCAATGGGAGTGAATTCAAATGTGTTTCCAGGTTTAAACACTCTTGGAGTTTCATTTGTTAGAGCAGATTTTGATGTTGGTGGTGTTAATGTTCCTCACTTTCATCCAAGAGCAACAGAAGTTGCTATTGTGTTGGAGGGAAAAATTTATTCAGGTTTTGTTGATACTAAGAACAAGATTTTTGCTAAAGTTTTGGAGAAAGGTGAGGTTATGGTTTTTCCAAGAGGACTTGTGCATTTTCAGATGAATGTTGGTGATGTTCCTGCTACTATTTTTGGTAGTTTTGATAGTCAAAATCCTGGTTTGATGAAAATTCCAAATGCTGTTTTTGGATCTGAGATTAAAGATGAGCTTTTGGAAAAAGCTTTTGGACTTAATTCTAAGGAGCTTTCTAAGTTAAAGAAGAGGTTTACTCCTAGCTAA

Protein sequence

>Msa1449910

MINNKVTILLVLMCFIIAAFCSDPDPVMDYCIAKSEDNKFSCKNSSTATVEDFTFSGIKLPGNFKETGFSSMGVNSNVFPGLNTLGVSFVRADFDVGGVNVPHFHPRATEVAIVLEGKIYSGFVDTKNKIFAKVLEKGEVMVFPRGLVHFQMNVGDVPATIFGSFDSQNPGLMKIPNAVFGSEIKDELLEKAFGLNSKELSKLKKRFTPS*