AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0780960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0780960 MtrunA17_Chr5g0441301 98.457 324 5 0 1 324 1 324 0.0 629
Msa0780960 MtrunA17_Chr3g0093721 78.526 312 56 3 1 312 1 301 3.34e-175 487
Msa0780960 MtrunA17_Chr8g0348171 29.730 148 98 1 35 182 4 145 6.13e-22 90.5
Msa0780960 MtrunA17_Chr7g0248741 27.703 148 101 1 35 182 3 144 1.47e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0780960 AT2G44060.2 73.817 317 75 2 1 310 1 316 3.57e-168 471
Msa0780960 AT2G44060.1 73.817 317 75 2 1 310 1 316 3.57e-168 471
Msa0780960 AT2G46140.1 28.125 160 113 1 23 182 3 160 5.23e-16 75.1
Msa0780960 AT1G01470.1 27.333 150 103 1 33 182 2 145 2.05e-15 72.8

Find 82 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATTTAGGCCTAAGGATTT+TGG 0.201634 5_2:-63159331 Msa0780960:CDS
TTTGAATTATGATGATATTA+AGG 0.211230 5_2:-63159710 Msa0780960:CDS
ATTGAGGAAGTTATTGGATT+TGG 0.215319 5_2:-63159958 Msa0780960:CDS
GAGAAGATTGAGGAAGTTAT+TGG 0.246466 5_2:-63159964 Msa0780960:CDS
TCCTTAGGCCTAAATGTAAT+TGG 0.253200 5_2:+63159337 None:intergenic
AACATCAATGTTACCTTTCA+TGG 0.276977 5_2:+63159264 None:intergenic
TTGATGTTCCAATTACATTT+AGG 0.280314 5_2:-63159345 Msa0780960:CDS
TTGAGGAAGTTATTGGATTT+GGG 0.288159 5_2:-63159957 Msa0780960:CDS
GATGACAGGAAACTCATTTC+CGG 0.298886 5_2:-63159796 Msa0780960:CDS
TGCAAATCTTCATTTGAAAC+TGG 0.307693 5_2:-63159497 Msa0780960:CDS
AGATCAACCTTAACTCTATA+AGG 0.319233 5_2:+63159652 None:intergenic
CTATAAGGAATTATAGTTCC+AGG 0.330332 5_2:+63159667 None:intergenic
CAATCCGGTGCCAATTCCTT+TGG 0.345867 5_2:-63159845 Msa0780960:CDS
AAAGGAATTGGCACCGGATT+GGG 0.352381 5_2:+63159847 None:intergenic
ATATCAACATCAGGCTTATA+AGG 0.355807 5_2:+63159562 None:intergenic
ACTTTGATTGGATTTGATCT+TGG 0.362296 5_2:-63160150 None:intergenic
GACAAGCTTGTGCTACCTTT+TGG 0.364890 5_2:-63158416 Msa0780960:three_prime_UTR
TTATTTGTCTTGTGACTTTG+TGG 0.367989 5_2:-63158353 Msa0780960:three_prime_UTR
TATAATTCCTTATAGAGTTA+AGG 0.372128 5_2:-63159659 Msa0780960:CDS
ATTGATGTTGACACTCCCTT+TGG 0.400916 5_2:-63159250 Msa0780960:CDS
GCATTATGGGATATGATTAG+AGG 0.402300 5_2:-63159304 Msa0780960:CDS
TTGGATTTGATCTTGGCTAC+AGG 0.406841 5_2:-63160143 None:intergenic
ATTATTCATTTATTCGATGC+AGG 0.408351 5_2:-63158323 Msa0780960:three_prime_UTR
TTGATGTCAACCAAAGGAAT+TGG 0.409107 5_2:+63159835 None:intergenic
GTACCGCCTTCTTTGCTGAT+AGG 0.410076 5_2:+63159214 None:intergenic
TCTCAATGCACTTGAGTATG+AGG 0.411463 5_2:-63159449 Msa0780960:CDS
AAGACAAATAAATTTAAAAC+TGG 0.418476 5_2:+63158365 None:intergenic
TCATCATAATTCAAAGTAAG+AGG 0.423200 5_2:+63159718 None:intergenic
TCCAATTACATTTAGGCCTA+AGG 0.432912 5_2:-63159338 Msa0780960:CDS
TAAACAAGAAGAAGAAGAGA+AGG 0.434343 5_2:-63160031 Msa0780960:CDS
TAAGCCGGAAGTGGTGGAAA+GGG 0.443472 5_2:-63160088 Msa0780960:CDS
GAAGGGTGGATTTATTGAAA+AGG 0.450744 5_2:-63160013 Msa0780960:CDS
CAAAGGAATTGGCACCGGAT+TGG 0.450750 5_2:+63159846 None:intergenic
GTCGACATCGGAGGATAAGC+CGG 0.458014 5_2:-63160103 Msa0780960:CDS
GGCAATTTCATTGCTCCAAA+GGG 0.463990 5_2:+63159235 None:intergenic
CTTGGTGATGTTAACATTGG+AGG 0.465706 5_2:-63159421 Msa0780960:CDS
ACATCAGGCTTATAAGGAAT+CGG 0.468874 5_2:+63159568 None:intergenic
ATGCACTTGAGTATGAGGTT+TGG 0.473664 5_2:-63159444 Msa0780960:CDS
TGGGATATGATTAGAGGAAA+GGG 0.479770 5_2:-63159298 Msa0780960:CDS
CAGCTGTAATGAGTTTCTAC+AGG 0.480386 5_2:+63158619 None:intergenic
ACCTATGCTGATATTAAACC+TGG 0.480676 5_2:-63159685 Msa0780960:CDS
ATGATTAGAGGAAAGGGAAC+TGG 0.488011 5_2:-63159292 Msa0780960:CDS
ACCTGCATCTGGTATCAAAC+CGG 0.498397 5_2:+63159777 None:intergenic
AAAGATTTCATTCATGACAT+TGG 0.506615 5_2:-63159988 Msa0780960:CDS
AAAGTTCTCTTTCGAAGAGA+CGG 0.507989 5_2:-63159521 Msa0780960:CDS
CTTGAGTATGAGGTTTGGCT+TGG 0.511412 5_2:-63159439 Msa0780960:CDS
ACTCAAGTGCATTGAGACCT+AGG 0.519423 5_2:+63159455 None:intergenic
TTGATGTTCCTGTCTTGGGA+CGG 0.522854 5_2:-63159624 Msa0780960:CDS
GCATGGATAGTACCTGCATC+TGG 0.527119 5_2:+63159766 None:intergenic
TGACATTGGTGAGAAGATTG+AGG 0.527714 5_2:-63159974 Msa0780960:CDS
ATAAGCCGGAAGTGGTGGAA+AGG 0.527851 5_2:-63160089 Msa0780960:CDS
TTGTCATATATCTCACCAAA+AGG 0.531081 5_2:+63158401 None:intergenic
TGGATTTGATCTTGGCTACA+GGG 0.531138 5_2:-63160142 None:intergenic
TCCGGTTTGATACCAGATGC+AGG 0.531490 5_2:-63159778 Msa0780960:CDS
ATGGGATATGATTAGAGGAA+AGG 0.531523 5_2:-63159299 Msa0780960:CDS
CAACAAGATCTATCTTATGA+AGG 0.535657 5_2:+63159890 None:intergenic
AATGCAGAACCAAAATCCTT+AGG 0.539184 5_2:+63159322 None:intergenic
ATTTGATTGAGAGTGATGAC+AGG 0.540955 5_2:-63159810 Msa0780960:CDS
GTAAGTATGTCCGTTCAGTC+AGG 0.541046 5_2:-63158488 Msa0780960:three_prime_UTR
GCAGGTACTATCCATGCTCA+TGG 0.541882 5_2:-63159760 Msa0780960:CDS
TTATTCATTTATTCGATGCA+GGG 0.543770 5_2:-63158322 Msa0780960:three_prime_UTR
AAATTGCCTATCAGCAAAGA+AGG 0.549361 5_2:-63159220 Msa0780960:CDS
TGGCTTGGTGATGTTAACAT+TGG 0.549882 5_2:-63159424 Msa0780960:CDS
ATCGGAGGATAAGCCGGAAG+TGG 0.555417 5_2:-63160097 Msa0780960:CDS
CAAGAAGAAGAAGAGAAGGG+TGG 0.556521 5_2:-63160027 Msa0780960:CDS
TCCAGGTTTAATATCAGCAT+AGG 0.559190 5_2:+63159684 None:intergenic
AAACAAGAAGAAGAAGAGAA+GGG 0.561708 5_2:-63160030 Msa0780960:CDS
CAGTTTCCTCTCCATGAGCA+TGG 0.563307 5_2:+63159749 None:intergenic
GGAGGATAAGCCGGAAGTGG+TGG 0.573380 5_2:-63160094 Msa0780960:CDS
GGAACTGGTTACACCATGAA+AGG 0.575081 5_2:-63159277 Msa0780960:CDS
AAAGCACATGCCTGACTGAA+CGG 0.584988 5_2:+63158478 None:intergenic
TCTCATCAAGAATCCCAATC+CGG 0.585530 5_2:-63159860 Msa0780960:CDS
AGGCAATTTCATTGCTCCAA+AGG 0.588624 5_2:+63159234 None:intergenic
AAGCCGGAAGTGGTGGAAAG+GGG 0.589381 5_2:-63160087 Msa0780960:CDS
TCAACCAAAGGAATTGGCAC+CGG 0.602356 5_2:+63159841 None:intergenic
AGCCGGAAGTGGTGGAAAGG+GGG 0.604743 5_2:-63160086 Msa0780960:CDS
TACTATCCATGCTCATGGAG+AGG 0.617564 5_2:-63159755 Msa0780960:CDS
ATCTGATATGTCGACATCGG+AGG 0.623245 5_2:-63160112 Msa0780960:exon
AAATAATTGATGTCAACCAA+AGG 0.624235 5_2:+63159829 None:intergenic
TTGATCTGATATGTCGACAT+CGG 0.657528 5_2:-63160115 Msa0780960:exon
TTGCCTATCAGCAAAGAAGG+CGG 0.700915 5_2:-63159217 Msa0780960:CDS
TGATGACGATGACGATGACG+AGG 0.712621 5_2:-63159158 Msa0780960:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAATTATGATGATATTA+AGG - chr5_2:63158696-63158715 Msa0780960:intron 15.0%
!! TTGATCTTGTAAAATTAATT+TGG - chr5_2:63159491-63159510 Msa0780960:CDS 15.0%
!!! AGCAATAAAAAGTAATTTTT+GGG + chr5_2:63159610-63159629 None:intergenic 15.0%
!!! AAGCAATAAAAAGTAATTTT+TGG + chr5_2:63159611-63159630 None:intergenic 15.0%
!!! AAGACAAATAAATTTAAAAC+TGG + chr5_2:63160044-63160063 None:intergenic 15.0%
!! TTTGAATTATGATGATATTA+AGG - chr5_2:63158696-63158715 Msa0780960:intron 15.0%
!! TTGATCTTGTAAAATTAATT+TGG - chr5_2:63159491-63159510 Msa0780960:CDS 15.0%
!!! AGCAATAAAAAGTAATTTTT+GGG + chr5_2:63159610-63159629 None:intergenic 15.0%
!!! AAGCAATAAAAAGTAATTTT+TGG + chr5_2:63159611-63159630 None:intergenic 15.0%
!!! AAGACAAATAAATTTAAAAC+TGG + chr5_2:63160044-63160063 None:intergenic 15.0%
!!! ATCTTATGAAGGTTAATTTT+AGG + chr5_2:63158508-63158527 None:intergenic 20.0%
!! TATAATTCCTTATAGAGTTA+AGG - chr5_2:63158747-63158766 Msa0780960:intron 20.0%
!!! TAAAAAGTAATTTTTGGGTT+AGG + chr5_2:63159605-63159624 None:intergenic 20.0%
!!! CATCAAACTTTATTTTCTAA+TGG + chr5_2:63159836-63159855 None:intergenic 20.0%
!!! ATCTTATGAAGGTTAATTTT+AGG + chr5_2:63158508-63158527 None:intergenic 20.0%
!! TATAATTCCTTATAGAGTTA+AGG - chr5_2:63158747-63158766 Msa0780960:intron 20.0%
!!! TAAAAAGTAATTTTTGGGTT+AGG + chr5_2:63159605-63159624 None:intergenic 20.0%
!!! CATCAAACTTTATTTTCTAA+TGG + chr5_2:63159836-63159855 None:intergenic 20.0%
! AAAGATTTCATTCATGACAT+TGG - chr5_2:63158418-63158437 Msa0780960:three_prime_UTR 25.0%
! AAATAATTGATGTCAACCAA+AGG + chr5_2:63158580-63158599 None:intergenic 25.0%
! TCATCATAATTCAAAGTAAG+AGG + chr5_2:63158691-63158710 None:intergenic 25.0%
!!! ATTTCTCCTTTTTTCTCTAA+AGG + chr5_2:63158817-63158836 None:intergenic 25.0%
!! ATTTTGTCAATATCAACATC+AGG + chr5_2:63158856-63158875 None:intergenic 25.0%
! TTGATGTTCCAATTACATTT+AGG - chr5_2:63159061-63159080 Msa0780960:intron 25.0%
! ACAACATGCAAAATATAGAT+AGG + chr5_2:63159301-63159320 None:intergenic 25.0%
! TTAGTATGCAACTATACATA+TGG - chr5_2:63159351-63159370 Msa0780960:CDS 25.0%
! CAGATTTAACTGAACAAATT+AGG - chr5_2:63159389-63159408 Msa0780960:CDS 25.0%
!!! GTGTTTTTACTTTAAACGTA+AGG - chr5_2:63159549-63159568 Msa0780960:CDS 25.0%
! AAGCAAATACAGTATACAAA+AGG + chr5_2:63159724-63159743 None:intergenic 25.0%
!!! CTTTATTTTCTAATGGACAT+GGG + chr5_2:63159829-63159848 None:intergenic 25.0%
!!! ACTTTATTTTCTAATGGACA+TGG + chr5_2:63159830-63159849 None:intergenic 25.0%
! ATTATTCATTTATTCGATGC+AGG - chr5_2:63160083-63160102 Msa0780960:CDS 25.0%
! TTATTCATTTATTCGATGCA+GGG - chr5_2:63160084-63160103 Msa0780960:CDS 25.0%
! AAAGATTTCATTCATGACAT+TGG - chr5_2:63158418-63158437 Msa0780960:three_prime_UTR 25.0%
! AAATAATTGATGTCAACCAA+AGG + chr5_2:63158580-63158599 None:intergenic 25.0%
! TCATCATAATTCAAAGTAAG+AGG + chr5_2:63158691-63158710 None:intergenic 25.0%
!!! ATTTCTCCTTTTTTCTCTAA+AGG + chr5_2:63158817-63158836 None:intergenic 25.0%
!! ATTTTGTCAATATCAACATC+AGG + chr5_2:63158856-63158875 None:intergenic 25.0%
! TTGATGTTCCAATTACATTT+AGG - chr5_2:63159061-63159080 Msa0780960:intron 25.0%
! ACAACATGCAAAATATAGAT+AGG + chr5_2:63159301-63159320 None:intergenic 25.0%
! TTAGTATGCAACTATACATA+TGG - chr5_2:63159351-63159370 Msa0780960:CDS 25.0%
! CAGATTTAACTGAACAAATT+AGG - chr5_2:63159389-63159408 Msa0780960:CDS 25.0%
!!! GTGTTTTTACTTTAAACGTA+AGG - chr5_2:63159549-63159568 Msa0780960:CDS 25.0%
! AAGCAAATACAGTATACAAA+AGG + chr5_2:63159724-63159743 None:intergenic 25.0%
!!! CTTTATTTTCTAATGGACAT+GGG + chr5_2:63159829-63159848 None:intergenic 25.0%
!!! ACTTTATTTTCTAATGGACA+TGG + chr5_2:63159830-63159849 None:intergenic 25.0%
! ATTATTCATTTATTCGATGC+AGG - chr5_2:63160083-63160102 Msa0780960:CDS 25.0%
! TTATTCATTTATTCGATGCA+GGG - chr5_2:63160084-63160103 Msa0780960:CDS 25.0%
!! TTTTAAGGATGATAAGCACA+AGG - chr5_2:63158345-63158364 Msa0780960:three_prime_UTR 30.0%
TAAACAAGAAGAAGAAGAGA+AGG - chr5_2:63158375-63158394 Msa0780960:three_prime_UTR 30.0%
AAACAAGAAGAAGAAGAGAA+GGG - chr5_2:63158376-63158395 Msa0780960:three_prime_UTR 30.0%
ATTGAGGAAGTTATTGGATT+TGG - chr5_2:63158448-63158467 Msa0780960:three_prime_UTR 30.0%
TTGAGGAAGTTATTGGATTT+GGG - chr5_2:63158449-63158468 Msa0780960:three_prime_UTR 30.0%
CAACAAGATCTATCTTATGA+AGG + chr5_2:63158519-63158538 None:intergenic 30.0%
CTATAAGGAATTATAGTTCC+AGG + chr5_2:63158742-63158761 None:intergenic 30.0%
AGATCAACCTTAACTCTATA+AGG + chr5_2:63158757-63158776 None:intergenic 30.0%
!! TATTTTTGATGTTCCTGTCT+TGG - chr5_2:63158777-63158796 Msa0780960:intron 30.0%
! ATTTTTGATGTTCCTGTCTT+GGG - chr5_2:63158778-63158797 Msa0780960:intron 30.0%
ATATCAACATCAGGCTTATA+AGG + chr5_2:63158847-63158866 None:intergenic 30.0%
! TGCAAATCTTCATTTGAAAC+TGG - chr5_2:63158909-63158928 Msa0780960:intron 30.0%
!! AACATGAATGATTTTGACCT+AGG - chr5_2:63158934-63158953 Msa0780960:intron 30.0%
TCTGCTAAACTTGAGAAAAA+TGG - chr5_2:63159024-63159043 Msa0780960:intron 30.0%
AACATCAATGTTACCTTTCA+TGG + chr5_2:63159145-63159164 None:intergenic 30.0%
TACCCGTCTTAAGAAAAATA+GGG - chr5_2:63159215-63159234 Msa0780960:CDS 30.0%
!! TTCCCTATTTTTCTTAAGAC+GGG + chr5_2:63159220-63159239 None:intergenic 30.0%
!! CTTCCCTATTTTTCTTAAGA+CGG + chr5_2:63159221-63159240 None:intergenic 30.0%
CTTAAGAAAAATAGGGAAGA+TGG - chr5_2:63159222-63159241 Msa0780960:CDS 30.0%
! ACATTACCGAGAATTAGTTT+AGG + chr5_2:63159421-63159440 None:intergenic 30.0%
AATGTAACCAAGAATGTGTT+TGG - chr5_2:63159436-63159455 Msa0780960:CDS 30.0%
!!! AAGTAATTTTTGGGTTAGGT+TGG + chr5_2:63159601-63159620 None:intergenic 30.0%
TTGTCATATATCTCACCAAA+AGG + chr5_2:63160008-63160027 None:intergenic 30.0%
TTATTTGTCTTGTGACTTTG+TGG - chr5_2:63160053-63160072 Msa0780960:CDS 30.0%
!! TTTTAAGGATGATAAGCACA+AGG - chr5_2:63158345-63158364 Msa0780960:three_prime_UTR 30.0%
TAAACAAGAAGAAGAAGAGA+AGG - chr5_2:63158375-63158394 Msa0780960:three_prime_UTR 30.0%
AAACAAGAAGAAGAAGAGAA+GGG - chr5_2:63158376-63158395 Msa0780960:three_prime_UTR 30.0%
ATTGAGGAAGTTATTGGATT+TGG - chr5_2:63158448-63158467 Msa0780960:three_prime_UTR 30.0%
TTGAGGAAGTTATTGGATTT+GGG - chr5_2:63158449-63158468 Msa0780960:three_prime_UTR 30.0%
CAACAAGATCTATCTTATGA+AGG + chr5_2:63158519-63158538 None:intergenic 30.0%
CTATAAGGAATTATAGTTCC+AGG + chr5_2:63158742-63158761 None:intergenic 30.0%
AGATCAACCTTAACTCTATA+AGG + chr5_2:63158757-63158776 None:intergenic 30.0%
!! TATTTTTGATGTTCCTGTCT+TGG - chr5_2:63158777-63158796 Msa0780960:intron 30.0%
! ATTTTTGATGTTCCTGTCTT+GGG - chr5_2:63158778-63158797 Msa0780960:intron 30.0%
ATATCAACATCAGGCTTATA+AGG + chr5_2:63158847-63158866 None:intergenic 30.0%
! TGCAAATCTTCATTTGAAAC+TGG - chr5_2:63158909-63158928 Msa0780960:intron 30.0%
!! AACATGAATGATTTTGACCT+AGG - chr5_2:63158934-63158953 Msa0780960:intron 30.0%
TCTGCTAAACTTGAGAAAAA+TGG - chr5_2:63159024-63159043 Msa0780960:intron 30.0%
AACATCAATGTTACCTTTCA+TGG + chr5_2:63159145-63159164 None:intergenic 30.0%
TACCCGTCTTAAGAAAAATA+GGG - chr5_2:63159215-63159234 Msa0780960:CDS 30.0%
!! TTCCCTATTTTTCTTAAGAC+GGG + chr5_2:63159220-63159239 None:intergenic 30.0%
!! CTTCCCTATTTTTCTTAAGA+CGG + chr5_2:63159221-63159240 None:intergenic 30.0%
CTTAAGAAAAATAGGGAAGA+TGG - chr5_2:63159222-63159241 Msa0780960:CDS 30.0%
! ACATTACCGAGAATTAGTTT+AGG + chr5_2:63159421-63159440 None:intergenic 30.0%
AATGTAACCAAGAATGTGTT+TGG - chr5_2:63159436-63159455 Msa0780960:CDS 30.0%
!!! AAGTAATTTTTGGGTTAGGT+TGG + chr5_2:63159601-63159620 None:intergenic 30.0%
TTGTCATATATCTCACCAAA+AGG + chr5_2:63160008-63160027 None:intergenic 30.0%
TTATTTGTCTTGTGACTTTG+TGG - chr5_2:63160053-63160072 Msa0780960:CDS 30.0%
TTGATCTGATATGTCGACAT+CGG - chr5_2:63158291-63158310 Msa0780960:three_prime_UTR 35.0%
! GAAGGGTGGATTTATTGAAA+AGG - chr5_2:63158393-63158412 Msa0780960:three_prime_UTR 35.0%
GAGAAGATTGAGGAAGTTAT+TGG - chr5_2:63158442-63158461 Msa0780960:three_prime_UTR 35.0%
!! ATCGTTTTAACATCAGCAGT+TGG + chr5_2:63158478-63158497 None:intergenic 35.0%
TTGATGTCAACCAAAGGAAT+TGG + chr5_2:63158574-63158593 None:intergenic 35.0%
ATTTGATTGAGAGTGATGAC+AGG - chr5_2:63158596-63158615 Msa0780960:three_prime_UTR 35.0%
ACCTATGCTGATATTAAACC+TGG - chr5_2:63158721-63158740 Msa0780960:intron 35.0%
TCCAGGTTTAATATCAGCAT+AGG + chr5_2:63158725-63158744 None:intergenic 35.0%
! ACGCTACCTTTAGAGAAAAA+AGG - chr5_2:63158808-63158827 Msa0780960:intron 35.0%
ACATCAGGCTTATAAGGAAT+CGG + chr5_2:63158841-63158860 None:intergenic 35.0%
AAAGTTCTCTTTCGAAGAGA+CGG - chr5_2:63158885-63158904 Msa0780960:intron 35.0%
GCTAAACTTGAGAAAAATGG+AGG - chr5_2:63159027-63159046 Msa0780960:intron 35.0%
TCCAATTACATTTAGGCCTA+AGG - chr5_2:63159068-63159087 Msa0780960:intron 35.0%
TCCTTAGGCCTAAATGTAAT+TGG + chr5_2:63159072-63159091 None:intergenic 35.0%
! ACATTTAGGCCTAAGGATTT+TGG - chr5_2:63159075-63159094 Msa0780960:intron 35.0%
AATGCAGAACCAAAATCCTT+AGG + chr5_2:63159087-63159106 None:intergenic 35.0%
!!! AGGATTTTGGTTCTGCATTA+TGG - chr5_2:63159088-63159107 Msa0780960:intron 35.0%
!!! GGATTTTGGTTCTGCATTAT+GGG - chr5_2:63159089-63159108 Msa0780960:intron 35.0%
GCATTATGGGATATGATTAG+AGG - chr5_2:63159102-63159121 Msa0780960:intron 35.0%
ATGGGATATGATTAGAGGAA+AGG - chr5_2:63159107-63159126 Msa0780960:intron 35.0%
TGGGATATGATTAGAGGAAA+GGG - chr5_2:63159108-63159127 Msa0780960:intron 35.0%
AAATTGCCTATCAGCAAAGA+AGG - chr5_2:63159186-63159205 Msa0780960:CDS 35.0%
CTACCCGTCTTAAGAAAAAT+AGG - chr5_2:63159214-63159233 Msa0780960:CDS 35.0%
AAGAAAAATAGGGAAGATGG+AGG - chr5_2:63159225-63159244 Msa0780960:CDS 35.0%
CTGTAAGACTTGTAACATCT+TGG + chr5_2:63159324-63159343 None:intergenic 35.0%
CGCTTTCCTAAACTAATTCT+CGG - chr5_2:63159412-63159431 Msa0780960:CDS 35.0%
CACTATTCCAAACACATTCT+TGG + chr5_2:63159446-63159465 None:intergenic 35.0%
! GTGTATTCAATCTCTTTTGC+AGG - chr5_2:63159756-63159775 Msa0780960:CDS 35.0%
TTGATCTGATATGTCGACAT+CGG - chr5_2:63158291-63158310 Msa0780960:three_prime_UTR 35.0%
! GAAGGGTGGATTTATTGAAA+AGG - chr5_2:63158393-63158412 Msa0780960:three_prime_UTR 35.0%
GAGAAGATTGAGGAAGTTAT+TGG - chr5_2:63158442-63158461 Msa0780960:three_prime_UTR 35.0%
!! ATCGTTTTAACATCAGCAGT+TGG + chr5_2:63158478-63158497 None:intergenic 35.0%
TTGATGTCAACCAAAGGAAT+TGG + chr5_2:63158574-63158593 None:intergenic 35.0%
ATTTGATTGAGAGTGATGAC+AGG - chr5_2:63158596-63158615 Msa0780960:three_prime_UTR 35.0%
ACCTATGCTGATATTAAACC+TGG - chr5_2:63158721-63158740 Msa0780960:intron 35.0%
TCCAGGTTTAATATCAGCAT+AGG + chr5_2:63158725-63158744 None:intergenic 35.0%
! ACGCTACCTTTAGAGAAAAA+AGG - chr5_2:63158808-63158827 Msa0780960:intron 35.0%
ACATCAGGCTTATAAGGAAT+CGG + chr5_2:63158841-63158860 None:intergenic 35.0%
AAAGTTCTCTTTCGAAGAGA+CGG - chr5_2:63158885-63158904 Msa0780960:intron 35.0%
GCTAAACTTGAGAAAAATGG+AGG - chr5_2:63159027-63159046 Msa0780960:intron 35.0%
TCCAATTACATTTAGGCCTA+AGG - chr5_2:63159068-63159087 Msa0780960:intron 35.0%
TCCTTAGGCCTAAATGTAAT+TGG + chr5_2:63159072-63159091 None:intergenic 35.0%
! ACATTTAGGCCTAAGGATTT+TGG - chr5_2:63159075-63159094 Msa0780960:intron 35.0%
AATGCAGAACCAAAATCCTT+AGG + chr5_2:63159087-63159106 None:intergenic 35.0%
!!! AGGATTTTGGTTCTGCATTA+TGG - chr5_2:63159088-63159107 Msa0780960:intron 35.0%
!!! GGATTTTGGTTCTGCATTAT+GGG - chr5_2:63159089-63159108 Msa0780960:intron 35.0%
GCATTATGGGATATGATTAG+AGG - chr5_2:63159102-63159121 Msa0780960:intron 35.0%
ATGGGATATGATTAGAGGAA+AGG - chr5_2:63159107-63159126 Msa0780960:intron 35.0%
TGGGATATGATTAGAGGAAA+GGG - chr5_2:63159108-63159127 Msa0780960:intron 35.0%
AAATTGCCTATCAGCAAAGA+AGG - chr5_2:63159186-63159205 Msa0780960:CDS 35.0%
CTACCCGTCTTAAGAAAAAT+AGG - chr5_2:63159214-63159233 Msa0780960:CDS 35.0%
AAGAAAAATAGGGAAGATGG+AGG - chr5_2:63159225-63159244 Msa0780960:CDS 35.0%
CTGTAAGACTTGTAACATCT+TGG + chr5_2:63159324-63159343 None:intergenic 35.0%
CGCTTTCCTAAACTAATTCT+CGG - chr5_2:63159412-63159431 Msa0780960:CDS 35.0%
CACTATTCCAAACACATTCT+TGG + chr5_2:63159446-63159465 None:intergenic 35.0%
! GTGTATTCAATCTCTTTTGC+AGG - chr5_2:63159756-63159775 Msa0780960:CDS 35.0%
TGACATTGGTGAGAAGATTG+AGG - chr5_2:63158432-63158451 Msa0780960:three_prime_UTR 40.0%
TCTCATCAAGAATCCCAATC+CGG - chr5_2:63158546-63158565 Msa0780960:three_prime_UTR 40.0%
GATGACAGGAAACTCATTTC+CGG - chr5_2:63158610-63158629 Msa0780960:three_prime_UTR 40.0%
TCTCAATGCACTTGAGTATG+AGG - chr5_2:63158957-63158976 Msa0780960:intron 40.0%
ATGCACTTGAGTATGAGGTT+TGG - chr5_2:63158962-63158981 Msa0780960:intron 40.0%
!! TGGCTTGGTGATGTTAACAT+TGG - chr5_2:63158982-63159001 Msa0780960:intron 40.0%
!! CTTGGTGATGTTAACATTGG+AGG - chr5_2:63158985-63159004 Msa0780960:intron 40.0%
ATGATTAGAGGAAAGGGAAC+TGG - chr5_2:63159114-63159133 Msa0780960:intron 40.0%
ATTGATGTTGACACTCCCTT+TGG - chr5_2:63159156-63159175 Msa0780960:intron 40.0%
GGCAATTTCATTGCTCCAAA+GGG + chr5_2:63159174-63159193 None:intergenic 40.0%
AGGCAATTTCATTGCTCCAA+AGG + chr5_2:63159175-63159194 None:intergenic 40.0%
ATCGAGTTACCTCCTAATCT+CGG + chr5_2:63159643-63159662 None:intergenic 40.0%
CAGCTGTAATGAGTTTCTAC+AGG + chr5_2:63159790-63159809 None:intergenic 40.0%
TGACATTGGTGAGAAGATTG+AGG - chr5_2:63158432-63158451 Msa0780960:three_prime_UTR 40.0%
TCTCATCAAGAATCCCAATC+CGG - chr5_2:63158546-63158565 Msa0780960:three_prime_UTR 40.0%
GATGACAGGAAACTCATTTC+CGG - chr5_2:63158610-63158629 Msa0780960:three_prime_UTR 40.0%
TCTCAATGCACTTGAGTATG+AGG - chr5_2:63158957-63158976 Msa0780960:intron 40.0%
ATGCACTTGAGTATGAGGTT+TGG - chr5_2:63158962-63158981 Msa0780960:intron 40.0%
!! TGGCTTGGTGATGTTAACAT+TGG - chr5_2:63158982-63159001 Msa0780960:intron 40.0%
!! CTTGGTGATGTTAACATTGG+AGG - chr5_2:63158985-63159004 Msa0780960:intron 40.0%
ATGATTAGAGGAAAGGGAAC+TGG - chr5_2:63159114-63159133 Msa0780960:intron 40.0%
ATTGATGTTGACACTCCCTT+TGG - chr5_2:63159156-63159175 Msa0780960:intron 40.0%
GGCAATTTCATTGCTCCAAA+GGG + chr5_2:63159174-63159193 None:intergenic 40.0%
AGGCAATTTCATTGCTCCAA+AGG + chr5_2:63159175-63159194 None:intergenic 40.0%
ATCGAGTTACCTCCTAATCT+CGG + chr5_2:63159643-63159662 None:intergenic 40.0%
CAGCTGTAATGAGTTTCTAC+AGG + chr5_2:63159790-63159809 None:intergenic 40.0%
ATCTGATATGTCGACATCGG+AGG - chr5_2:63158294-63158313 Msa0780960:three_prime_UTR 45.0%
CAAGAAGAAGAAGAGAAGGG+TGG - chr5_2:63158379-63158398 Msa0780960:three_prime_UTR 45.0%
! AAAGGAATTGGCACCGGATT+GGG + chr5_2:63158562-63158581 None:intergenic 45.0%
TCAACCAAAGGAATTGGCAC+CGG + chr5_2:63158568-63158587 None:intergenic 45.0%
ACCTGCATCTGGTATCAAAC+CGG + chr5_2:63158632-63158651 None:intergenic 45.0%
TACTATCCATGCTCATGGAG+AGG - chr5_2:63158651-63158670 Msa0780960:intron 45.0%
TTGATGTTCCTGTCTTGGGA+CGG - chr5_2:63158782-63158801 Msa0780960:intron 45.0%
ACTCAAGTGCATTGAGACCT+AGG + chr5_2:63158954-63158973 None:intergenic 45.0%
CTTGAGTATGAGGTTTGGCT+TGG - chr5_2:63158967-63158986 Msa0780960:intron 45.0%
GGAACTGGTTACACCATGAA+AGG - chr5_2:63159129-63159148 Msa0780960:intron 45.0%
TTGCCTATCAGCAAAGAAGG+CGG - chr5_2:63159189-63159208 Msa0780960:CDS 45.0%
CTTCTAGTCAAGCCGAGATT+AGG - chr5_2:63159628-63159647 Msa0780960:CDS 45.0%
AGCCAAACACACACTAGAGT+AGG - chr5_2:63159676-63159695 Msa0780960:CDS 45.0%
GACCTACTCTAGTGTGTGTT+TGG + chr5_2:63159681-63159700 None:intergenic 45.0%
GTAAGTATGTCCGTTCAGTC+AGG - chr5_2:63159918-63159937 Msa0780960:CDS 45.0%
AAAGCACATGCCTGACTGAA+CGG + chr5_2:63159931-63159950 None:intergenic 45.0%
! GACAAGCTTGTGCTACCTTT+TGG - chr5_2:63159990-63160009 Msa0780960:CDS 45.0%
ATCTGATATGTCGACATCGG+AGG - chr5_2:63158294-63158313 Msa0780960:three_prime_UTR 45.0%
CAAGAAGAAGAAGAGAAGGG+TGG - chr5_2:63158379-63158398 Msa0780960:three_prime_UTR 45.0%
! AAAGGAATTGGCACCGGATT+GGG + chr5_2:63158562-63158581 None:intergenic 45.0%
TCAACCAAAGGAATTGGCAC+CGG + chr5_2:63158568-63158587 None:intergenic 45.0%
ACCTGCATCTGGTATCAAAC+CGG + chr5_2:63158632-63158651 None:intergenic 45.0%
TACTATCCATGCTCATGGAG+AGG - chr5_2:63158651-63158670 Msa0780960:intron 45.0%
TTGATGTTCCTGTCTTGGGA+CGG - chr5_2:63158782-63158801 Msa0780960:intron 45.0%
ACTCAAGTGCATTGAGACCT+AGG + chr5_2:63158954-63158973 None:intergenic 45.0%
CTTGAGTATGAGGTTTGGCT+TGG - chr5_2:63158967-63158986 Msa0780960:intron 45.0%
GGAACTGGTTACACCATGAA+AGG - chr5_2:63159129-63159148 Msa0780960:intron 45.0%
TTGCCTATCAGCAAAGAAGG+CGG - chr5_2:63159189-63159208 Msa0780960:CDS 45.0%
CTTCTAGTCAAGCCGAGATT+AGG - chr5_2:63159628-63159647 Msa0780960:CDS 45.0%
AGCCAAACACACACTAGAGT+AGG - chr5_2:63159676-63159695 Msa0780960:CDS 45.0%
GACCTACTCTAGTGTGTGTT+TGG + chr5_2:63159681-63159700 None:intergenic 45.0%
GTAAGTATGTCCGTTCAGTC+AGG - chr5_2:63159918-63159937 Msa0780960:CDS 45.0%
AAAGCACATGCCTGACTGAA+CGG + chr5_2:63159931-63159950 None:intergenic 45.0%
! GACAAGCTTGTGCTACCTTT+TGG - chr5_2:63159990-63160009 Msa0780960:CDS 45.0%
! ATAAGCCGGAAGTGGTGGAA+AGG - chr5_2:63158317-63158336 Msa0780960:three_prime_UTR 50.0%
! TAAGCCGGAAGTGGTGGAAA+GGG - chr5_2:63158318-63158337 Msa0780960:three_prime_UTR 50.0%
! GGTGGAAAGGGGGCTTTTTA+AGG - chr5_2:63158330-63158349 Msa0780960:three_prime_UTR 50.0%
! CAAAGGAATTGGCACCGGAT+TGG + chr5_2:63158563-63158582 None:intergenic 50.0%
! CAATCCGGTGCCAATTCCTT+TGG - chr5_2:63158561-63158580 Msa0780960:three_prime_UTR 50.0%
! TCCGGTTTGATACCAGATGC+AGG - chr5_2:63158628-63158647 Msa0780960:exon 50.0%
GCATGGATAGTACCTGCATC+TGG + chr5_2:63158643-63158662 None:intergenic 50.0%
GCAGGTACTATCCATGCTCA+TGG - chr5_2:63158646-63158665 Msa0780960:intron 50.0%
CAGTTTCCTCTCCATGAGCA+TGG + chr5_2:63158660-63158679 None:intergenic 50.0%
GTACCGCCTTCTTTGCTGAT+AGG + chr5_2:63159195-63159214 None:intergenic 50.0%
! TGATGACGATGACGATGACG+AGG - chr5_2:63159248-63159267 Msa0780960:CDS 50.0%
CTAGTCAAGCCGAGATTAGG+AGG - chr5_2:63159631-63159650 Msa0780960:CDS 50.0%
! ATAAGCCGGAAGTGGTGGAA+AGG - chr5_2:63158317-63158336 Msa0780960:three_prime_UTR 50.0%
! TAAGCCGGAAGTGGTGGAAA+GGG - chr5_2:63158318-63158337 Msa0780960:three_prime_UTR 50.0%
! GGTGGAAAGGGGGCTTTTTA+AGG - chr5_2:63158330-63158349 Msa0780960:three_prime_UTR 50.0%
! CAAAGGAATTGGCACCGGAT+TGG + chr5_2:63158563-63158582 None:intergenic 50.0%
! CAATCCGGTGCCAATTCCTT+TGG - chr5_2:63158561-63158580 Msa0780960:three_prime_UTR 50.0%
! TCCGGTTTGATACCAGATGC+AGG - chr5_2:63158628-63158647 Msa0780960:exon 50.0%
GCATGGATAGTACCTGCATC+TGG + chr5_2:63158643-63158662 None:intergenic 50.0%
GCAGGTACTATCCATGCTCA+TGG - chr5_2:63158646-63158665 Msa0780960:intron 50.0%
CAGTTTCCTCTCCATGAGCA+TGG + chr5_2:63158660-63158679 None:intergenic 50.0%
GTACCGCCTTCTTTGCTGAT+AGG + chr5_2:63159195-63159214 None:intergenic 50.0%
! TGATGACGATGACGATGACG+AGG - chr5_2:63159248-63159267 Msa0780960:CDS 50.0%
CTAGTCAAGCCGAGATTAGG+AGG - chr5_2:63159631-63159650 Msa0780960:CDS 50.0%
GTCGACATCGGAGGATAAGC+CGG - chr5_2:63158303-63158322 Msa0780960:three_prime_UTR 55.0%
ATCGGAGGATAAGCCGGAAG+TGG - chr5_2:63158309-63158328 Msa0780960:three_prime_UTR 55.0%
! AAGCCGGAAGTGGTGGAAAG+GGG - chr5_2:63158319-63158338 Msa0780960:three_prime_UTR 55.0%
AGCGTAAACCGTCCCAAGAC+AGG + chr5_2:63158793-63158812 None:intergenic 55.0%
GTCGACATCGGAGGATAAGC+CGG - chr5_2:63158303-63158322 Msa0780960:three_prime_UTR 55.0%
ATCGGAGGATAAGCCGGAAG+TGG - chr5_2:63158309-63158328 Msa0780960:three_prime_UTR 55.0%
! AAGCCGGAAGTGGTGGAAAG+GGG - chr5_2:63158319-63158338 Msa0780960:three_prime_UTR 55.0%
AGCGTAAACCGTCCCAAGAC+AGG + chr5_2:63158793-63158812 None:intergenic 55.0%
GGAGGATAAGCCGGAAGTGG+TGG - chr5_2:63158312-63158331 Msa0780960:three_prime_UTR 60.0%
! AGCCGGAAGTGGTGGAAAGG+GGG - chr5_2:63158320-63158339 Msa0780960:three_prime_UTR 60.0%
AGCCCCCTTTCCACCACTTC+CGG + chr5_2:63158325-63158344 None:intergenic 60.0%
GGAGGATAAGCCGGAAGTGG+TGG - chr5_2:63158312-63158331 Msa0780960:three_prime_UTR 60.0%
! AGCCGGAAGTGGTGGAAAGG+GGG - chr5_2:63158320-63158339 Msa0780960:three_prime_UTR 60.0%
AGCCCCCTTTCCACCACTTC+CGG + chr5_2:63158325-63158344 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 63158270 63160158 63158270 ID=Msa0780960;Name=Msa0780960
chr5_2 mRNA 63158270 63160158 63158270 ID=Msa0780960-mRNA-1;Parent=Msa0780960;Name=Msa0780960-mRNA-1;_AED=0.05;_eAED=0.05;_QI=31|1|1|1|0|0.5|2|375|324
chr5_2 exon 63158270 63158650 63158270 ID=Msa0780960-mRNA-1:exon:18796;Parent=Msa0780960-mRNA-1
chr5_2 exon 63159159 63160158 63159159 ID=Msa0780960-mRNA-1:exon:18795;Parent=Msa0780960-mRNA-1
chr5_2 five_prime_UTR 63160128 63160158 63160128 ID=Msa0780960-mRNA-1:five_prime_utr;Parent=Msa0780960-mRNA-1
chr5_2 CDS 63159159 63160127 63159159 ID=Msa0780960-mRNA-1:cds;Parent=Msa0780960-mRNA-1
chr5_2 CDS 63158645 63158650 63158645 ID=Msa0780960-mRNA-1:cds;Parent=Msa0780960-mRNA-1
chr5_2 three_prime_UTR 63158270 63158644 63158270 ID=Msa0780960-mRNA-1:three_prime_utr;Parent=Msa0780960-mRNA-1
Gene Sequence

>Msa0780960

ATGTCGACATCGGAGGATAAGCCGGAAGTGGTGGAAAGGGGGCTTTTTAAGGATGATAAGCACAAGGAAGAAGATAAACAAGAAGAAGAAGAGAAGGGTGGATTTATTGAAAAGGTGAAAGATTTCATTCATGACATTGGTGAGAAGATTGAGGAAGTTATTGGATTTGGGAAACCAACTGCTGATGTTAAAACGATACACGTTCCTAAAATTAACCTTCATAAGATAGATCTTGTTGTTGATATTCTCATCAAGAATCCCAATCCGGTGCCAATTCCTTTGGTTGACATCAATTATTTGATTGAGAGTGATGACAGGAAACTCATTTCCGGTTTGATACCAGATGCAGGTACTATCCATGCTCATGGAGAGGAAACTGTCAAGATTCCTCTTACTTTGAATTATGATGATATTAAGGAAACCTATGCTGATATTAAACCTGGAACTATAATTCCTTATAGAGTTAAGGTTGATCTTATTTTTGATGTTCCTGTCTTGGGACGGTTTACGCTACCTTTAGAGAAAAAAGGAGAAATTCCGATTCCTTATAAGCCTGATGTTGATATTGACAAAATTCAATTCAAAAAGTTCTCTTTCGAAGAGACGGTTGCAAATCTTCATTTGAAACTGGAAAACATGAATGATTTTGACCTAGGTCTCAATGCACTTGAGTATGAGGTTTGGCTTGGTGATGTTAACATTGGAGGTGCACAGCTCAGTAAATCTGCTAAACTTGAGAAAAATGGAGGAATTAGTTACATTGATGTTCCAATTACATTTAGGCCTAAGGATTTTGGTTCTGCATTATGGGATATGATTAGAGGAAAGGGAACTGGTTACACCATGAAAGGTAACATTGATGTTGACACTCCCTTTGGAGCAATGAAATTGCCTATCAGCAAAGAAGGCGGTACTACCCGTCTTAAGAAAAATAGGGAAGATGGAGGTGATGACGATGACGATGACGAGGATTGA
Protein sequence

>Msa0780960

MSTSEDKPEVVERGLFKDDKHKEEDKQEEEEKGGFIEKVKDFIHDIGEKIEEVIGFGKPTADVKTIHVPKINLHKIDLVVDILIKNPNPVPIPLVDINYLIESDDRKLISGLIPDAGTIHAHGEETVKIPLTLNYDDIKETYADIKPGTIIPYRVKVDLIFDVPVLGRFTLPLEKKGEIPIPYKPDVDIDKIQFKKFSFEETVANLHLKLENMNDFDLGLNALEYEVWLGDVNIGGAQLSKSAKLEKNGGISYIDVPITFRPKDFGSALWDMIRGKGTGYTMKGNIDVDTPFGAMKLPISKEGGTTRLKKNREDGGDDDDDDED*