AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0819540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0819540 MtrunA17_Chr5g0438731 98.624 436 5 1 1 435 32 467 0.0 879
Msa0819540 MtrunA17_Chr2g0287471 84.962 133 20 0 1 133 1 133 4.50e-73 233
Msa0819540 MtrunA17_Chr2g0287471 44.037 109 60 1 156 264 26 133 3.20e-23 100
Msa0819540 MtrunA17_Chr7g0271721 34.074 270 152 8 1 267 3 249 1.23e-39 145
Msa0819540 MtrunA17_Chr7g0271721 42.308 130 67 4 157 280 23 150 2.13e-21 95.1
Msa0819540 MtrunA17_Chr1g0194961 36.161 224 123 5 33 251 34 242 1.68e-39 145
Msa0819540 MtrunA17_Chr1g0194961 40.541 148 70 4 28 172 147 279 1.67e-24 103
Msa0819540 MtrunA17_Chr1g0194961 40.625 128 68 4 163 284 34 159 2.07e-19 89.4
Msa0819540 MtrunA17_Chr1g0200731 40.351 114 59 4 30 136 95 206 9.02e-17 82.4
Msa0819540 MtrunA17_Chr1g0200731 28.700 223 135 5 163 382 98 299 7.76e-15 76.3
Msa0819540 MtrunA17_Chr3g0124851 41.284 109 57 4 30 132 35 142 1.29e-16 82.0
Msa0819540 MtrunA17_Chr3g0124851 34.884 172 93 7 163 329 38 195 1.85e-16 81.6
Msa0819540 MtrunA17_Chr3g0124851 33.333 105 68 1 26 128 375 479 7.96e-12 67.0
Msa0819540 MtrunA17_Chr2g0326581 41.600 125 65 4 135 256 55 174 1.27e-14 75.9
Msa0819540 MtrunA17_Chr2g0326581 51.220 82 38 2 30 110 80 160 2.92e-13 71.6
Msa0819540 MtrunA17_Chr2g0326581 27.184 206 132 6 64 259 322 519 1.12e-11 66.6
Msa0819540 MtrunA17_Chr2g0326581 32.609 138 87 3 36 169 424 559 1.30e-11 66.6
Msa0819540 MtrunA17_Chr8g0360031 41.463 82 48 0 30 111 73 154 2.22e-14 71.2
Msa0819540 MtrunA17_Chr8g0360031 25.654 191 122 6 72 259 2 175 6.39e-13 67.0
Msa0819540 MtrunA17_Chr2g0317731 26.846 149 99 2 107 245 9 157 6.21e-14 69.7
Msa0819540 MtrunA17_Chr2g0317731 34.653 101 66 0 11 111 51 151 2.56e-13 67.8
Msa0819540 MtrunA17_Chr2g0326241 28.814 118 81 2 17 132 29 145 9.35e-14 68.9
Msa0819540 MtrunA17_Chr2g0326241 29.167 120 82 2 156 273 37 155 1.08e-12 65.9
Msa0819540 MtrunA17_Chr1g0211871 34.483 116 72 2 24 138 64 176 1.13e-13 69.3
Msa0819540 MtrunA17_Chr1g0211871 30.631 111 73 1 153 263 63 169 7.26e-13 67.0
Msa0819540 MtrunA17_Chr1g0201781 32.558 86 58 0 34 119 66 151 5.24e-13 67.0
Msa0819540 MtrunA17_Chr1g0201781 28.846 104 67 3 166 268 68 165 6.62e-11 60.8
Msa0819540 MtrunA17_Chr4g0029181 48.421 95 40 5 30 120 102 191 1.16e-12 69.7
Msa0819540 MtrunA17_Chr2g0317721 34.513 113 68 1 5 111 10 122 4.14e-12 63.5
Msa0819540 MtrunA17_Chr3g0125821 35.632 87 56 0 33 119 73 159 7.45e-12 63.9
Msa0819540 MtrunA17_Chr2g0317711 33.628 113 69 1 5 111 10 122 1.29e-11 62.4
Msa0819540 MtrunA17_Chr4g0041801 32.967 91 52 2 160 242 143 232 1.63e-11 65.9
Msa0819540 MtrunA17_Chr4g0044731 36.905 84 50 1 31 111 63 146 4.51e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0819540 AT1G04980.1 72.523 444 112 4 1 435 5 447 0.0 678
Msa0819540 AT2G32920.1 73.474 426 108 3 14 435 16 440 0.0 654
Msa0819540 AT2G47470.2 36.638 232 127 5 33 259 28 244 1.19e-43 154
Msa0819540 AT2G47470.2 54.369 103 42 3 30 128 143 244 1.59e-27 110
Msa0819540 AT2G47470.2 39.370 127 71 3 163 284 28 153 1.96e-19 88.2
Msa0819540 AT2G47470.3 36.638 232 127 5 33 259 28 244 2.26e-43 155
Msa0819540 AT2G47470.3 54.369 103 42 3 30 128 143 244 1.64e-27 112
Msa0819540 AT2G47470.3 39.370 127 71 3 163 284 28 153 4.30e-19 88.2
Msa0819540 AT2G47470.4 36.638 232 127 5 33 259 28 244 2.35e-43 155
Msa0819540 AT2G47470.4 54.369 103 42 3 30 128 143 244 1.76e-27 112
Msa0819540 AT2G47470.4 39.370 127 71 3 163 284 28 153 4.23e-19 88.6
Msa0819540 AT2G47470.1 36.638 232 127 5 33 259 28 244 3.13e-43 155
Msa0819540 AT2G47470.1 54.369 103 42 3 30 128 143 244 1.88e-27 112
Msa0819540 AT2G47470.1 39.370 127 71 3 163 284 28 153 4.74e-19 88.6
Msa0819540 AT3G54960.2 46.154 91 48 1 30 120 101 190 1.09e-18 88.6
Msa0819540 AT3G54960.2 28.182 220 136 6 163 380 104 303 3.81e-15 77.8
Msa0819540 AT3G54960.1 46.154 91 48 1 30 120 101 190 1.45e-18 88.6
Msa0819540 AT3G54960.1 39.362 94 55 2 163 256 104 195 4.54e-15 77.8
Msa0819540 AT3G54960.1 37.363 91 49 2 36 126 445 527 3.59e-11 65.5
Msa0819540 AT5G60640.1 36.493 211 111 10 30 226 105 306 2.38e-17 84.7
Msa0819540 AT5G60640.1 42.424 99 49 4 161 256 106 199 4.34e-15 77.8
Msa0819540 AT5G60640.1 26.733 202 130 5 68 259 351 544 5.95e-12 68.2
Msa0819540 AT5G60640.2 36.493 211 111 10 30 226 105 306 2.82e-17 84.3
Msa0819540 AT5G60640.2 42.424 99 49 4 161 256 106 199 5.61e-15 77.4
Msa0819540 AT5G60640.2 26.633 199 129 5 62 252 346 535 2.67e-11 65.9
Msa0819540 AT5G60640.3 36.493 211 111 10 30 226 105 306 2.98e-17 84.3
Msa0819540 AT5G60640.3 42.424 99 49 4 161 256 106 199 3.47e-15 78.2
Msa0819540 AT5G60640.3 34.737 95 60 1 167 259 386 480 3.88e-11 65.5
Msa0819540 AT1G21750.2 40.367 109 58 4 30 132 32 139 3.87e-17 84.0
Msa0819540 AT1G21750.2 35.849 106 62 3 163 263 35 139 1.45e-14 75.9
Msa0819540 AT1G21750.2 37.113 97 59 1 26 120 372 468 1.14e-12 70.1
Msa0819540 AT1G21750.1 40.367 109 58 4 30 132 32 139 4.04e-17 84.0
Msa0819540 AT1G21750.1 29.518 166 99 5 163 323 35 187 1.66e-14 75.9
Msa0819540 AT1G21750.1 37.113 97 59 1 26 120 372 468 1.03e-12 70.1
Msa0819540 AT1G21750.1 37.113 97 58 2 165 259 381 476 3.88e-11 65.5
Msa0819540 AT1G77510.1 28.421 190 117 6 152 336 23 198 2.03e-16 81.6
Msa0819540 AT1G77510.1 39.450 109 59 4 30 132 31 138 3.02e-16 81.3
Msa0819540 AT1G77510.1 27.165 254 157 11 14 252 227 467 5.40e-11 65.1
Msa0819540 AT1G07960.4 30.000 120 81 2 28 144 25 144 1.61e-14 71.2
Msa0819540 AT1G07960.4 24.561 114 83 2 154 265 21 133 2.20e-13 67.8
Msa0819540 AT1G07960.3 30.000 120 81 2 28 144 25 144 1.61e-14 71.2
Msa0819540 AT1G07960.3 24.561 114 83 2 154 265 21 133 2.20e-13 67.8
Msa0819540 AT1G07960.1 30.000 120 81 2 28 144 25 144 1.61e-14 71.2
Msa0819540 AT1G07960.1 24.561 114 83 2 154 265 21 133 2.20e-13 67.8
Msa0819540 AT1G07960.2 30.000 120 81 2 28 144 25 144 1.61e-14 71.2
Msa0819540 AT1G07960.2 24.561 114 83 2 154 265 21 133 2.20e-13 67.8
Msa0819540 AT1G35620.1 40.426 94 51 3 30 120 34 125 2.30e-14 75.1
Msa0819540 AT1G35620.1 27.966 236 138 10 155 383 29 239 9.00e-14 73.2
Msa0819540 AT1G43560.1 37.209 86 54 0 34 119 64 149 1.62e-13 68.9
Msa0819540 AT1G76760.1 31.429 105 68 1 34 138 69 169 2.02e-13 68.6
Msa0819540 AT4G03520.1 36.082 97 59 2 151 245 74 169 3.61e-12 65.5
Msa0819540 AT3G15360.1 36.667 90 57 0 30 119 88 177 4.97e-12 65.1
Msa0819540 AT1G50320.1 30.000 150 89 4 121 263 30 170 5.52e-12 64.7
Msa0819540 AT4G27080.1 32.000 125 67 4 126 242 118 232 2.33e-11 65.9
Msa0819540 AT4G27080.2 32.000 125 67 4 126 242 170 284 2.84e-11 65.9
Msa0819540 AT1G03680.1 26.923 156 94 4 110 245 8 163 2.94e-11 62.4
Msa0819540 AT3G20560.1 34.000 100 57 2 151 242 134 232 9.43e-11 63.9

Find 147 sgRNAs with CRISPR-Local

Find 316 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTCCCTTTCAAATTAT+TGG 0.104762 5_3:+56039348 None:intergenic
TTCAATCAATTGTTCATTAA+TGG 0.149709 5_3:+56037488 None:intergenic
TTCCCAACAATCTTGGTATT+TGG 0.173156 5_3:-56038930 Msa0819540:CDS
TTCCTTCCTTCCCGACATTT+TGG 0.184244 5_3:-56038650 Msa0819540:CDS
CTTAATTCCCTTCAGGAATA+TGG 0.210374 5_3:-56040134 Msa0819540:intron
TACGAGAAGACCTGGTTCTT+TGG 0.276020 5_3:-56037459 Msa0819540:three_prime_UTR
GAAGGCAGCTACTGTATTAA+AGG 0.276220 5_3:-56041082 Msa0819540:CDS
GATAGTATGTAGAATATTAA+AGG 0.283108 5_3:-56037681 Msa0819540:three_prime_UTR
CTCTAGTAACTTCGATGAAT+TGG 0.285509 5_3:-56039575 Msa0819540:CDS
CTTCAGGAATATGGTATTAG+AGG 0.289766 5_3:-56040125 Msa0819540:intron
CTATATAGCTCTGTCACTTC+TGG 0.297987 5_3:+56038810 None:intergenic
CGCAGAATTTGCACTTCAAC+AGG 0.299216 5_3:-56040027 Msa0819540:intron
TCAATCAATTGTTCATTAAT+GGG 0.300305 5_3:+56037489 None:intergenic
GTTTCCTTATAGTTCATCTT+TGG 0.305542 5_3:+56037765 None:intergenic
TTTGAAAGGGAAGGTTAAAC+TGG 0.309941 5_3:-56039341 Msa0819540:CDS
GTAAGGCTCTGACTCCTATA+TGG 0.316417 5_3:-56041107 Msa0819540:CDS
TGCGATTGGTTTAACATCTC+TGG 0.351595 5_3:+56040046 None:intergenic
AATTTATATAGACTCGTAAA+TGG 0.356732 5_3:+56037161 None:intergenic
CTCAAGGTCTGGCTGCTTAC+CGG 0.356762 5_3:+56038145 None:intergenic
GCAAAAGGGGCTTAGATATA+GGG 0.370495 5_3:+56037127 None:intergenic
TAGAGGATTTCCAACTATAA+AGG 0.370686 5_3:-56040108 Msa0819540:CDS
GTGGCTTGAAACTCGTATTA+AGG 0.371667 5_3:-56037344 Msa0819540:three_prime_UTR
AACACCTTTCTTAACGTTCA+AGG 0.373568 5_3:+56038079 None:intergenic
TGCAAAAGGGGCTTAGATAT+AGG 0.373607 5_3:+56037126 None:intergenic
GCTCGAACGTTGCATCATAA+AGG 0.379435 5_3:+56037286 None:intergenic
CTCGAACGTTGCATCATAAA+GGG 0.386422 5_3:+56037287 None:intergenic
TCAACCAAAGATGAACTATA+AGG 0.387123 5_3:-56037769 Msa0819540:exon
AAAGTTGGTGTTGGTGGGTA+TGG 0.390826 5_3:-56038119 Msa0819540:CDS
CAACGTTCGAGCTATGTTTA+CGG 0.396118 5_3:-56037275 Msa0819540:three_prime_UTR
GGGTCTTGAACAGCTATGTA+TGG 0.397598 5_3:-56038175 Msa0819540:intron
GTTGAAGAAGCGTAGATTGA+TGG 0.407509 5_3:+56042675 None:intergenic
CTAGCACCCTCGTAAGGAAT+TGG 0.409040 5_3:+56038891 None:intergenic
CATCTCCTATAGCATAAAAC+TGG 0.409206 5_3:+56037703 None:intergenic
TCAAGGATTCCCAACAATCT+TGG 0.416145 5_3:-56038937 Msa0819540:CDS
TCCATGGTGCGGGCACTGTA+AGG 0.416505 5_3:-56041124 Msa0819540:CDS
ACCTTGGACTTGAAGTTGTT+AGG 0.419712 5_3:+56042549 None:intergenic
TTGTTGAATTCTTTGCTCCA+TGG 0.424265 5_3:-56041140 Msa0819540:CDS
TGCTGATGCTCACCAATCTC+TGG 0.427343 5_3:-56041034 Msa0819540:CDS
AGTTCAAAAGGAGTCCTTAC+AGG 0.430959 5_3:-56038570 Msa0819540:intron
CTGGCTCCTTGGTAATCAAC+TGG 0.433932 5_3:+56040065 None:intergenic
AGGAATTGGAGTATCTTTAT+CGG 0.435399 5_3:+56038905 None:intergenic
AGAAACCACCGCTTCTTCTT+CGG 0.438238 5_3:-56039608 Msa0819540:CDS
TCTGTTTCTCCCCTCCGCCT+TGG 0.439079 5_3:+56038624 None:intergenic
AGAGAATTTGTGAAAGAAGC+TGG 0.439854 5_3:-56037928 Msa0819540:CDS
GTGTTGTCTATAGGATGTTT+TGG 0.440808 5_3:-56038707 Msa0819540:intron
TTATCAAAAGCAAAGAACTT+TGG 0.443575 5_3:-56039552 Msa0819540:CDS
TTTGTCAGTCTCTAATGAGC+AGG 0.447245 5_3:-56038968 Msa0819540:intron
GGTGCAAACACCTTTATAGT+TGG 0.448420 5_3:+56040098 None:intergenic
CCAACTTTCTGCTCAAGGTC+TGG 0.450805 5_3:+56038134 None:intergenic
AACCGTAGAGAGCGTGAGAA+AGG 0.451953 5_3:+56042598 None:intergenic
TGCTCACCAATCTCTGGCTC+AGG 0.454025 5_3:-56041028 Msa0819540:intron
GTTTAACATCTCTGGCTCCT+TGG 0.454708 5_3:+56040054 None:intergenic
TGTTGAATGTTGCACACAAC+CGG 0.455377 5_3:+56037415 None:intergenic
ATGAGCAGGTTCAATGTTCA+AGG 0.456443 5_3:-56038954 Msa0819540:CDS
AATTCTTTGCTCCATGGTGC+GGG 0.458259 5_3:-56041134 Msa0819540:CDS
GAATCTGCGAGTGATGAAAA+TGG 0.461683 5_3:+56042700 None:intergenic
ATCATTCGCACTAGATCAGC+TGG 0.462465 5_3:-56038850 Msa0819540:CDS
TTATAGTTCATCTTTGGTTG+AGG 0.465932 5_3:+56037771 None:intergenic
TGTCAGGTTCTGAATTCAAA+TGG 0.470995 5_3:-56041171 Msa0819540:intron
CTCCAAATACCAAGATTGTT+GGG 0.472339 5_3:+56038928 None:intergenic
GGAAAACCTGAGCCAGAGAT+TGG 0.473756 5_3:+56041022 None:intergenic
CGGTTAAATGGAAAAGCAAC+AGG 0.474554 5_3:-56039649 Msa0819540:CDS
ATAGTATGTAGAATATTAAA+GGG 0.475068 5_3:-56037680 Msa0819540:three_prime_UTR
GCTCCAAATACCAAGATTGT+TGG 0.479181 5_3:+56038927 None:intergenic
AGTGAGTTGAAGAACGGGTG+AGG 0.481557 5_3:+56042572 None:intergenic
ACGTTCAAGGCTACTAATGC+TGG 0.481617 5_3:+56038092 None:intergenic
ATCCTTTCTCACGCTCTCTA+CGG 0.482498 5_3:-56042600 Msa0819540:CDS
CAGTTGCAGAGAAGTTCAAA+AGG 0.484219 5_3:-56038582 Msa0819540:CDS
CTCGTATTAAGGTGAATAAG+TGG 0.484744 5_3:-56037333 Msa0819540:three_prime_UTR
GGTTCTGTCTTTACAATGGT+GGG 0.490337 5_3:+56037865 None:intergenic
ACTATAAAGGTGTTTGCACC+TGG 0.493971 5_3:-56040095 Msa0819540:CDS
GCCTTGGAATCCAAAATGTC+GGG 0.495320 5_3:+56038640 None:intergenic
TTAGGAGTGAGTTGAAGAAC+GGG 0.495568 5_3:+56042567 None:intergenic
GAACCATGGGATGGAAAAGA+TGG 0.495841 5_3:-56037847 Msa0819540:CDS
TGGTTCTGTCTTTACAATGG+TGG 0.496152 5_3:+56037864 None:intergenic
CGTTCCTCATTGACCATAGC+CGG 0.496245 5_3:-56037434 Msa0819540:three_prime_UTR
TTAGTGAAGTGTTTAGAAGA+TGG 0.498830 5_3:-56037196 Msa0819540:three_prime_UTR
AGAGCATCCAATAATTTGAA+AGG 0.498898 5_3:-56039355 Msa0819540:CDS
GTTAGGAGTGAGTTGAAGAA+CGG 0.501891 5_3:+56042566 None:intergenic
CGCCTTGGAATCCAAAATGT+CGG 0.513014 5_3:+56038639 None:intergenic
GAAGCTGGACGTGGAGGCAA+AGG 0.515570 5_3:-56037913 Msa0819540:CDS
AAGGCAGCTACTGTATTAAA+GGG 0.517489 5_3:-56041081 Msa0819540:CDS
GAGCATCCAATAATTTGAAA+GGG 0.518744 5_3:-56039354 Msa0819540:CDS
GTAGCTGCCTTCTCCCATAT+AGG 0.519121 5_3:+56041093 None:intergenic
ATGTTGCACACAACCGGCTA+TGG 0.522020 5_3:+56037421 None:intergenic
TGGTGGGAGGAGTGTCACCT+AGG 0.522614 5_3:+56037881 None:intergenic
ATTAAAGACTAGTATAGTGA+TGG 0.524551 5_3:-56037599 Msa0819540:three_prime_UTR
ATCCAATAATTTGAAAGGGA+AGG 0.529134 5_3:-56039350 Msa0819540:CDS
TAAGGCTCTGACTCCTATAT+GGG 0.533987 5_3:-56041106 Msa0819540:CDS
CCAGACCTTGAGCAGAAAGT+TGG 0.535182 5_3:-56038134 Msa0819540:CDS
GGTGGGAGGAGTGTCACCTA+GGG 0.535529 5_3:+56037882 None:intergenic
GAATTCTTTGCTCCATGGTG+CGG 0.548779 5_3:-56041135 Msa0819540:CDS
GAGCAGAAAGTTGGTGTTGG+TGG 0.549595 5_3:-56038125 Msa0819540:CDS
TCCTAACAACTTCAAGTCCA+AGG 0.552476 5_3:-56042550 Msa0819540:intron
ATCCAAAATGTCGGGAAGGA+AGG 0.552840 5_3:+56038648 None:intergenic
CCATGGTTCTGTCTTTACAA+TGG 0.553296 5_3:+56037861 None:intergenic
GTAGCCTTGAACGTTAAGAA+AGG 0.556407 5_3:-56038083 Msa0819540:CDS
AACATACCTCAGGAAAATGT+TGG 0.556667 5_3:-56037533 Msa0819540:three_prime_UTR
AATCTACGCTTCTTCAACAA+TGG 0.558002 5_3:-56042670 Msa0819540:exon
GGTCTTGAACAGCTATGTAT+GGG 0.559621 5_3:-56038174 Msa0819540:intron
AAAAGATGGAGAAATAATTG+AGG 0.562273 5_3:-56037833 Msa0819540:CDS
TGAAGTGCAAATTCTGCGAT+TGG 0.562719 5_3:+56040032 None:intergenic
GCCTTACAGTGCCCGCACCA+TGG 0.567839 5_3:+56041123 None:intergenic
AACATTATTACGAGAAGACC+TGG 0.569546 5_3:-56037467 Msa0819540:three_prime_UTR
GTTCTTGTATGAAGAGAAGA+AGG 0.570808 5_3:+56042724 None:intergenic
CAACTGTTAATAACTGCTGA+AGG 0.571823 5_3:+56038599 None:intergenic
GCAGTTCTAGCACCCTCGTA+AGG 0.576634 5_3:+56038885 None:intergenic
CAACACCAACTTTCTGCTCA+AGG 0.577087 5_3:+56038129 None:intergenic
GCTCCTTGGTAATCAACTGG+TGG 0.580906 5_3:+56040068 None:intergenic
AGCAGAAAGTTGGTGTTGGT+GGG 0.581032 5_3:-56038124 Msa0819540:CDS
AAGCTGGACGTGGAGGCAAA+GGG 0.583780 5_3:-56037912 Msa0819540:CDS
TTTGTGAAAGAAGCTGGACG+TGG 0.587104 5_3:-56037922 Msa0819540:CDS
ATCTAGTGCGAATGATTCGA+TGG 0.588461 5_3:+56038857 None:intergenic
AGCTATGTATGGGCAGCTGC+CGG 0.590014 5_3:-56038164 Msa0819540:CDS
TTCATGTCATTCTCATTCAG+TGG 0.591892 5_3:-56037559 Msa0819540:three_prime_UTR
GGCTTAGATATAGGGACGGA+GGG 0.591898 5_3:+56037135 None:intergenic
TTAAAGACTAGTATAGTGAT+GGG 0.592543 5_3:-56037598 Msa0819540:three_prime_UTR
TCTGACTCCTATATGGGAGA+AGG 0.598675 5_3:-56041100 Msa0819540:CDS
TCTGTCTTTACAATGGTGGG+AGG 0.605063 5_3:+56037868 None:intergenic
TCGTATTAAGGTGAATAAGT+GGG 0.608145 5_3:-56037332 Msa0819540:three_prime_UTR
TAGTTCTACCGAAGAAGAAG+CGG 0.621191 5_3:+56039600 None:intergenic
AGTTCAAAAGCACTCTTGAG+AGG 0.621890 5_3:+56038050 None:intergenic
TCAGTGGTACAACATACCTC+AGG 0.622601 5_3:-56037543 Msa0819540:three_prime_UTR
CATTGTAAAGACAGAACCAT+GGG 0.623172 5_3:-56037860 Msa0819540:CDS
TTCTACCGAAGAAGAAGCGG+TGG 0.624355 5_3:+56039603 None:intergenic
GGCAAAGGGAACTTGCCCCT+AGG 0.627464 5_3:-56037898 Msa0819540:CDS
CTTGAGCAGAAAGTTGGTGT+TGG 0.628583 5_3:-56038128 Msa0819540:CDS
GAGGAACGATCCAAAGAACC+AGG 0.629610 5_3:+56037449 None:intergenic
TTGAAAGGGAAGGTTAAACT+GGG 0.631529 5_3:-56039340 Msa0819540:CDS
AACACTTCACTAATGCATGT+AGG 0.634863 5_3:+56037206 None:intergenic
TGGAATCCAAAATGTCGGGA+AGG 0.635224 5_3:+56038644 None:intergenic
GATACTCCAATTCCTTACGA+GGG 0.636183 5_3:-56038897 Msa0819540:CDS
TGTTGACTGTGATGCTGACA+AGG 0.636371 5_3:-56039314 Msa0819540:intron
GTAAAGACAGAACCATGGGA+TGG 0.640244 5_3:-56037856 Msa0819540:CDS
TATAGCTCTGTCACTTCTGG+AGG 0.640322 5_3:+56038813 None:intergenic
GTGAAAGAAGCTGGACGTGG+AGG 0.650513 5_3:-56037919 Msa0819540:CDS
GGGCTTAGATATAGGGACGG+AGG 0.661411 5_3:+56037134 None:intergenic
TTAAATGGAAAAGCAACAGG+TGG 0.661631 5_3:-56039646 Msa0819540:CDS
AGATACTCCAATTCCTTACG+AGG 0.665654 5_3:-56038898 Msa0819540:CDS
TCGAACGTTGCATCATAAAG+GGG 0.668066 5_3:+56037288 None:intergenic
GTGGGAGGAGTGTCACCTAG+GGG 0.673404 5_3:+56037883 None:intergenic
GAATATTAAAGGGAAATGTG+TGG 0.677310 5_3:-56037670 Msa0819540:three_prime_UTR
AAGACTAGTATAGTGATGGG+TGG 0.679435 5_3:-56037595 Msa0819540:three_prime_UTR
ACAACCGGCTATGGTCAATG+AGG 0.692842 5_3:+56037430 None:intergenic
AAGGGGCTTAGATATAGGGA+CGG 0.695087 5_3:+56037131 None:intergenic
CCATTGTAAAGACAGAACCA+TGG 0.708421 5_3:-56037861 Msa0819540:CDS
AAACCACCAGTTGATTACCA+AGG 0.716041 5_3:-56040071 Msa0819540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTCTAAAATTATTAT+CGG - chr5_3:56037803-56037822 Msa0819540:CDS 10.0%
!! GTAATAAATAATTGAAATAT+AGG + chr5_3:56040379-56040398 None:intergenic 10.0%
!! TATAAATAAATTGAAAAGTA+AGG + chr5_3:56040815-56040834 None:intergenic 10.0%
!! ATCATCTGAAAAAAATTAAT+AGG - chr5_3:56037660-56037679 Msa0819540:three_prime_UTR 15.0%
!! TTATACTATTGTATTATGAT+TGG - chr5_3:56038554-56038573 Msa0819540:intron 15.0%
!!! AAAATTATTCGTATTTTCTT+GGG + chr5_3:56038609-56038628 None:intergenic 15.0%
!!! AAAAATTATTCGTATTTTCT+TGG + chr5_3:56038610-56038629 None:intergenic 15.0%
!!! ATAAAATTGTTTGATGTTTT+AGG - chr5_3:56039390-56039409 Msa0819540:CDS 15.0%
!!! TAAAATTGTTTGATGTTTTA+GGG - chr5_3:56039391-56039410 Msa0819540:CDS 15.0%
!! AATATTTGATTGAAACTTTA+GGG + chr5_3:56039910-56039929 None:intergenic 15.0%
!! AAATATTTGATTGAAACTTT+AGG + chr5_3:56039911-56039930 None:intergenic 15.0%
!! TGAAAAATTACTACAAATAA+AGG - chr5_3:56040016-56040035 Msa0819540:intron 15.0%
!! AATTTGATTAATAACTTGAA+AGG - chr5_3:56040041-56040060 Msa0819540:CDS 15.0%
!! ATTTGATTAATAACTTGAAA+GGG - chr5_3:56040042-56040061 Msa0819540:CDS 15.0%
!! AAAAATAGACTTACATAATT+TGG + chr5_3:56041851-56041870 None:intergenic 15.0%
!! ATAGTATGTAGAATATTAAA+GGG - chr5_3:56042192-56042211 Msa0819540:intron 15.0%
!! ATTGAAAAAAGATTAAACCT+TGG + chr5_3:56037342-56037361 None:intergenic 20.0%
!! TACTAACTAAACTCAAATTA+AGG - chr5_3:56038283-56038302 Msa0819540:intron 20.0%
!!! AAATTATTCGTATTTTCTTG+GGG + chr5_3:56038608-56038627 None:intergenic 20.0%
!! AAATTTAAATACTGAATCTG+AGG + chr5_3:56038894-56038913 None:intergenic 20.0%
!! ACACATATTCATATTCATAA+AGG + chr5_3:56038975-56038994 None:intergenic 20.0%
!! TTATAGCTTATATGAAAAAC+AGG - chr5_3:56039202-56039221 Msa0819540:intron 20.0%
!!! ATATTTAAAATAGTTACAGG+AGG + chr5_3:56039325-56039344 None:intergenic 20.0%
!!! CACATATTTAAAATAGTTAC+AGG + chr5_3:56039328-56039347 None:intergenic 20.0%
!!! CTGTAACTATTTTAAATATG+TGG - chr5_3:56039326-56039345 Msa0819540:CDS 20.0%
!! AAGGAAAAACTTTACATATA+AGG + chr5_3:56039455-56039474 None:intergenic 20.0%
!! CACTAATGTAATTTATTCTT+TGG + chr5_3:56039515-56039534 None:intergenic 20.0%
!!! TGTCTTTTTTTATTTCTCAA+AGG - chr5_3:56040458-56040477 Msa0819540:intron 20.0%
!! TTTAACAAACTGTTATTGTT+CGG - chr5_3:56040667-56040686 Msa0819540:intron 20.0%
!! TCAGAATAATATAAACTGAA+AGG + chr5_3:56040871-56040890 None:intergenic 20.0%
!!! TCTTTCAAATTCAACTTTTT+CGG - chr5_3:56041395-56041414 Msa0819540:intron 20.0%
!! CATGTTTAAATCAAGATAAA+AGG - chr5_3:56041451-56041470 Msa0819540:intron 20.0%
!!! TTGCAGTTATTTTTAAATTG+CGG - chr5_3:56041533-56041552 Msa0819540:intron 20.0%
!! TACTTACATGGAAATATAAT+GGG - chr5_3:56041566-56041585 Msa0819540:intron 20.0%
!!! AATGGTTTTTTGTTTGAATT+TGG - chr5_3:56041676-56041695 Msa0819540:intron 20.0%
!!! ATGGTTTTTTGTTTGAATTT+GGG - chr5_3:56041677-56041696 Msa0819540:intron 20.0%
!!! CTAATTTTATGCTAAATAGT+TGG - chr5_3:56041871-56041890 Msa0819540:intron 20.0%
!! GATAGTATGTAGAATATTAA+AGG - chr5_3:56042191-56042210 Msa0819540:intron 20.0%
!!! TTTTTCAGATTTTTCTTGTT+CGG - chr5_3:56042249-56042268 Msa0819540:intron 20.0%
!! TCAATCAATTGTTCATTAAT+GGG + chr5_3:56042386-56042405 None:intergenic 20.0%
!! TTCAATCAATTGTTCATTAA+TGG + chr5_3:56042387-56042406 None:intergenic 20.0%
!!! TTTTTTTTAAAGTAGCATAG+TGG - chr5_3:56042509-56042528 Msa0819540:intron 20.0%
!! AATTTATATAGACTCGTAAA+TGG + chr5_3:56042714-56042733 None:intergenic 20.0%
! AAAAAACCAGCTAAAAAAGT+GGG + chr5_3:56037388-56037407 None:intergenic 25.0%
!!! TTTTAGCTGGTTTTTTCAAA+TGG - chr5_3:56037392-56037411 Msa0819540:three_prime_UTR 25.0%
!!! TTTAGCTGGTTTTTTCAAAT+GGG - chr5_3:56037393-56037412 Msa0819540:three_prime_UTR 25.0%
!! TTTTATGCTTGAAAACTATC+TGG - chr5_3:56037444-56037463 Msa0819540:three_prime_UTR 25.0%
! TTTATGCTTGAAAACTATCT+GGG - chr5_3:56037445-56037464 Msa0819540:three_prime_UTR 25.0%
!!! TATGGTTGATTTTATGATTC+TGG - chr5_3:56037538-56037557 Msa0819540:three_prime_UTR 25.0%
!!! ATGGTTGATTTTATGATTCT+GGG - chr5_3:56037539-56037558 Msa0819540:three_prime_UTR 25.0%
! TAGCACTAAAATTTAGTTGA+AGG - chr5_3:56037943-56037962 Msa0819540:intron 25.0%
!! AGGTAAAGTGATTTTATGTT+TGG - chr5_3:56037963-56037982 Msa0819540:intron 25.0%
!! AAGTGATTTTATGTTTGGAT+AGG - chr5_3:56037968-56037987 Msa0819540:intron 25.0%
!!! TCAAGTAGAATCTATTTTGA+AGG - chr5_3:56038150-56038169 Msa0819540:CDS 25.0%
! TGAAGAGCTTATGAAAATAA+GGG + chr5_3:56038415-56038434 None:intergenic 25.0%
! TTGAAGAGCTTATGAAAATA+AGG + chr5_3:56038416-56038435 None:intergenic 25.0%
! CAATAGTATAAAAGACTGTT+TGG + chr5_3:56038545-56038564 None:intergenic 25.0%
!! ATTAAGTTGTTAATCCCAAT+AGG - chr5_3:56039290-56039309 Msa0819540:intron 25.0%
!! TTAAGTTGTTAATCCCAATA+GGG - chr5_3:56039291-56039310 Msa0819540:intron 25.0%
! AGTGAGAATATCAATGTATT+GGG - chr5_3:56040111-56040130 Msa0819540:CDS 25.0%
! AATATCAATGTATTGGGATT+AGG - chr5_3:56040117-56040136 Msa0819540:CDS 25.0%
! ATATCAATGTATTGGGATTA+GGG - chr5_3:56040118-56040137 Msa0819540:CDS 25.0%
! TATAGTTTATTTGCATGTAC+AGG - chr5_3:56040180-56040199 Msa0819540:intron 25.0%
! TTATCAAAAGCAAAGAACTT+TGG - chr5_3:56040320-56040339 Msa0819540:intron 25.0%
!!! TTTTTTATTTCTCAAAGGTG+TGG - chr5_3:56040463-56040482 Msa0819540:intron 25.0%
! TCAGTTTATATTATTCTGAC+AGG - chr5_3:56040872-56040891 Msa0819540:intron 25.0%
! GTACTTACATGGAAATATAA+TGG - chr5_3:56041565-56041584 Msa0819540:intron 25.0%
! AAAAGATGGAGAAATAATTG+AGG - chr5_3:56042039-56042058 Msa0819540:intron 25.0%
! ATTAAAGACTAGTATAGTGA+TGG - chr5_3:56042273-56042292 Msa0819540:intron 25.0%
! TTAAAGACTAGTATAGTGAT+GGG - chr5_3:56042274-56042293 Msa0819540:intron 25.0%
GAAAAAACCAGCTAAAAAAG+TGG + chr5_3:56037389-56037408 None:intergenic 30.0%
! TGTTGATTAGTGTGTGAATA+TGG - chr5_3:56037520-56037539 Msa0819540:three_prime_UTR 30.0%
! TTTTATGATTCTGGGTTTTG+TGG - chr5_3:56037547-56037566 Msa0819540:three_prime_UTR 30.0%
AATATTATGCCATAAAGCAC+CGG - chr5_3:56037729-56037748 Msa0819540:three_prime_UTR 30.0%
GAAATTGTAGCTTCTAATTC+TGG + chr5_3:56038045-56038064 None:intergenic 30.0%
AAATAGATTCTACTTGAAGC+TGG + chr5_3:56038147-56038166 None:intergenic 30.0%
AACTCAAATTAAGGTCTGTT+TGG - chr5_3:56038292-56038311 Msa0819540:intron 30.0%
AATAAGGGGAAAACAACTTA+TGG + chr5_3:56038400-56038419 None:intergenic 30.0%
GAAGAGCTTATGAAAATAAG+GGG + chr5_3:56038414-56038433 None:intergenic 30.0%
! TATCTAGTTTGTAATGCATC+AGG - chr5_3:56038653-56038672 Msa0819540:CDS 30.0%
! TGTAATGCATCAGGTTTTTA+TGG - chr5_3:56038662-56038681 Msa0819540:CDS 30.0%
! GTAATGCATCAGGTTTTTAT+GGG - chr5_3:56038663-56038682 Msa0819540:CDS 30.0%
GAACCTGACATTATTCAAAA+TGG + chr5_3:56038691-56038710 None:intergenic 30.0%
AGGAATGAAGAAATGAACAA+TGG + chr5_3:56038874-56038893 None:intergenic 30.0%
TCGAATAAACCAATCTAAAC+AGG + chr5_3:56039084-56039103 None:intergenic 30.0%
GATATACAATATGTAGAGCA+TGG + chr5_3:56039433-56039452 None:intergenic 30.0%
! TTGGTTGCTTTACATAGAAT+GGG + chr5_3:56039496-56039515 None:intergenic 30.0%
! TTTGGTTGCTTTACATAGAA+TGG + chr5_3:56039497-56039516 None:intergenic 30.0%
CCTGTTATGATTTACATGTT+CGG - chr5_3:56039640-56039659 Msa0819540:CDS 30.0%
TCTAATACCATATTCCTGAA+GGG + chr5_3:56039748-56039767 None:intergenic 30.0%
TAGAGGATTTCCAACTATAA+AGG - chr5_3:56039764-56039783 Msa0819540:intron 30.0%
! TTTGATTGAAACTTTAGGGA+GGG + chr5_3:56039906-56039925 None:intergenic 30.0%
! ATTTGATTGAAACTTTAGGG+AGG + chr5_3:56039907-56039926 None:intergenic 30.0%
AATTGTAACACTACACATGT+TGG + chr5_3:56039934-56039953 None:intergenic 30.0%
ACATGTGTAGTGTTACAATT+TGG - chr5_3:56039934-56039953 Msa0819540:intron 30.0%
! AGATAGATGTCTTCTTTTTG+TGG - chr5_3:56039971-56039990 Msa0819540:intron 30.0%
GAGTGAGAATATCAATGTAT+TGG - chr5_3:56040110-56040129 Msa0819540:CDS 30.0%
TTATTTGCATGTACAGGTAA+AGG - chr5_3:56040186-56040205 Msa0819540:intron 30.0%
! AGAGCATCCAATAATTTGAA+AGG - chr5_3:56040517-56040536 Msa0819540:intron 30.0%
GAGCATCCAATAATTTGAAA+GGG - chr5_3:56040518-56040537 Msa0819540:intron 30.0%
ATCCAATAATTTGAAAGGGA+AGG - chr5_3:56040522-56040541 Msa0819540:intron 30.0%
AACCTTCCCTTTCAAATTAT+TGG + chr5_3:56040527-56040546 None:intergenic 30.0%
ATGCAGATAGCAAAAAATAG+CGG + chr5_3:56040772-56040791 None:intergenic 30.0%
! AGGAATTGGAGTATCTTTAT+CGG + chr5_3:56040970-56040989 None:intergenic 30.0%
TACAATCAAGTAAAGTTTGC+AGG + chr5_3:56041107-56041126 None:intergenic 30.0%
! TAAACATGAGTGTTGTCTAT+AGG - chr5_3:56041156-56041175 Msa0819540:CDS 30.0%
!!! TGTTTTTTTACTTGCCTGTA+AGG + chr5_3:56041319-56041338 None:intergenic 30.0%
ATTAACTCATGAGTCATAGA+GGG + chr5_3:56041376-56041395 None:intergenic 30.0%
!! GAAATATAATGGGTTCTCAT+TGG - chr5_3:56041576-56041595 Msa0819540:intron 30.0%
! ATTTTCTCTTGAAGAACTCA+TGG - chr5_3:56042069-56042088 Msa0819540:intron 30.0%
! TTTTCTCTTGAAGAACTCAT+GGG - chr5_3:56042070-56042089 Msa0819540:intron 30.0%
TTATAGTTCATCTTTGGTTG+AGG + chr5_3:56042104-56042123 None:intergenic 30.0%
TCAACCAAAGATGAACTATA+AGG - chr5_3:56042103-56042122 Msa0819540:intron 30.0%
GTTTCCTTATAGTTCATCTT+TGG + chr5_3:56042110-56042129 None:intergenic 30.0%
!! CATTACCAGTTTTATGCTAT+AGG - chr5_3:56042164-56042183 Msa0819540:intron 30.0%
GAATATTAAAGGGAAATGTG+TGG - chr5_3:56042202-56042221 Msa0819540:intron 30.0%
TCGTATTAAGGTGAATAAGT+GGG - chr5_3:56042540-56042559 Msa0819540:intron 30.0%
! TTAGTGAAGTGTTTAGAAGA+TGG - chr5_3:56042676-56042695 Msa0819540:five_prime_UTR 30.0%
GTTCTTGTATGAAGAGAAGA+AGG + chr5_3:56037151-56037170 None:intergenic 35.0%
AATCTACGCTTCTTCAACAA+TGG - chr5_3:56037202-56037221 Msa0819540:three_prime_UTR 35.0%
! TTTGCTATGTTGCTGTATTG+TGG - chr5_3:56037485-56037504 Msa0819540:three_prime_UTR 35.0%
CCATGTAAACAAATGTGTGT+CGG + chr5_3:56037776-56037795 None:intergenic 35.0%
ACACAACATCTATGAAAGCA+TGG + chr5_3:56037864-56037883 None:intergenic 35.0%
GATAGGTTCATGTAAAAGTG+AGG - chr5_3:56037985-56038004 Msa0819540:intron 35.0%
AAGTAGATTCTACTTGAAGC+TGG + chr5_3:56038067-56038086 None:intergenic 35.0%
GCTTCAAGTAGAATCTACTT+TGG - chr5_3:56038067-56038086 Msa0819540:CDS 35.0%
TCAAGTAGAATCTACTTTGG+AGG - chr5_3:56038070-56038089 Msa0819540:CDS 35.0%
GGCAAAATCAATTCTACTCT+AGG - chr5_3:56038171-56038190 Msa0819540:intron 35.0%
CTACTCTAGGTGATTCATTA+AGG - chr5_3:56038184-56038203 Msa0819540:intron 35.0%
! TATTCGTATTTTCTTGGGGA+GGG + chr5_3:56038604-56038623 None:intergenic 35.0%
! TTATTCGTATTTTCTTGGGG+AGG + chr5_3:56038605-56038624 None:intergenic 35.0%
!!! GTGCCATTTTGAATAATGTC+AGG - chr5_3:56038685-56038704 Msa0819540:CDS 35.0%
!! TGTCAGGTTCTGAATTCAAA+TGG - chr5_3:56038701-56038720 Msa0819540:intron 35.0%
TTGTTGAATTCTTTGCTCCA+TGG - chr5_3:56038732-56038751 Msa0819540:intron 35.0%
AAGGCAGCTACTGTATTAAA+GGG - chr5_3:56038791-56038810 Msa0819540:intron 35.0%
CATTTCTGTACAATGTACAC+TGG - chr5_3:56039009-56039028 Msa0819540:intron 35.0%
CTGGAAATACATACCTTTGA+TGG - chr5_3:56039028-56039047 Msa0819540:intron 35.0%
! GGTATTGAACCTGTTTAGAT+TGG - chr5_3:56039072-56039091 Msa0819540:intron 35.0%
TAAGTACCTGTTAGACAGTT+AGG - chr5_3:56039117-56039136 Msa0819540:intron 35.0%
AAGTACCTGTTAGACAGTTA+GGG - chr5_3:56039118-56039137 Msa0819540:intron 35.0%
TACAATATGTAGAGCATGGT+AGG + chr5_3:56039429-56039448 None:intergenic 35.0%
TTACATAGAATGGGAAGACT+AGG + chr5_3:56039487-56039506 None:intergenic 35.0%
CCGAACATGTAAATCATAAC+AGG + chr5_3:56039643-56039662 None:intergenic 35.0%
! GTCTTTTCTTAATTCCCTTC+AGG - chr5_3:56039731-56039750 Msa0819540:intron 35.0%
CTTAATTCCCTTCAGGAATA+TGG - chr5_3:56039738-56039757 Msa0819540:intron 35.0%
CTCTAATACCATATTCCTGA+AGG + chr5_3:56039749-56039768 None:intergenic 35.0%
CTTCAGGAATATGGTATTAG+AGG - chr5_3:56039747-56039766 Msa0819540:intron 35.0%
!! GGTTTGTTGGAATCTATTCT+TGG - chr5_3:56039866-56039885 Msa0819540:intron 35.0%
!! TAAAGGCTCTTTTGAAGGAA+CGG - chr5_3:56040203-56040222 Msa0819540:intron 35.0%
!! CTTTTGAAGGAACGGTTAAA+TGG - chr5_3:56040211-56040230 Msa0819540:intron 35.0%
TTAAATGGAAAAGCAACAGG+TGG - chr5_3:56040226-56040245 Msa0819540:intron 35.0%
CTCTAGTAACTTCGATGAAT+TGG - chr5_3:56040297-56040316 Msa0819540:intron 35.0%
! TTGTAGAATTTTTCGCACCT+TGG - chr5_3:56040344-56040363 Msa0819540:intron 35.0%
!! AATATAGGAAGTACTCACCA+AGG + chr5_3:56040364-56040383 None:intergenic 35.0%
GTAGAATTACATGTTCAGTC+AGG + chr5_3:56040401-56040420 None:intergenic 35.0%
GTAAAAAATTAGCTCCTGAG+TGG - chr5_3:56040491-56040510 Msa0819540:intron 35.0%
TTTGAAAGGGAAGGTTAAAC+TGG - chr5_3:56040531-56040550 Msa0819540:intron 35.0%
TTGAAAGGGAAGGTTAAACT+GGG - chr5_3:56040532-56040551 Msa0819540:intron 35.0%
TTCCCAACAATCTTGGTATT+TGG - chr5_3:56040942-56040961 Msa0819540:intron 35.0%
CTCCAAATACCAAGATTGTT+GGG + chr5_3:56040947-56040966 None:intergenic 35.0%
! GTGTTGTCTATAGGATGTTT+TGG - chr5_3:56041165-56041184 Msa0819540:CDS 35.0%
!!! GATGTTTTGGAAGAGAAATG+TGG - chr5_3:56041178-56041197 Msa0819540:intron 35.0%
CAACTGTTAATAACTGCTGA+AGG + chr5_3:56041276-56041295 None:intergenic 35.0%
GATTAACTCATGAGTCATAG+AGG + chr5_3:56041377-56041396 None:intergenic 35.0%
GTTTGATGAAGATGCATTGT+TGG + chr5_3:56041626-56041645 None:intergenic 35.0%
CTTTGTTGACGAATGCTTAA+TGG - chr5_3:56041658-56041677 Msa0819540:intron 35.0%
AACACCTTTCTTAACGTTCA+AGG + chr5_3:56041796-56041815 None:intergenic 35.0%
AGAGAATTTGTGAAAGAAGC+TGG - chr5_3:56041944-56041963 Msa0819540:intron 35.0%
CATTGTAAAGACAGAACCAT+GGG - chr5_3:56042012-56042031 Msa0819540:intron 35.0%
CATCTCCTATAGCATAAAAC+TGG + chr5_3:56042172-56042191 None:intergenic 35.0%
GAAAAATCTGAAAAACAGCG+AGG + chr5_3:56042244-56042263 None:intergenic 35.0%
TTCATGTCATTCTCATTCAG+TGG - chr5_3:56042313-56042332 Msa0819540:intron 35.0%
AACATACCTCAGGAAAATGT+TGG - chr5_3:56042339-56042358 Msa0819540:intron 35.0%
AACATTATTACGAGAAGACC+TGG - chr5_3:56042405-56042424 Msa0819540:intron 35.0%
CTCGTATTAAGGTGAATAAG+TGG - chr5_3:56042539-56042558 Msa0819540:intron 35.0%
AACACTTCACTAATGCATGT+AGG + chr5_3:56042669-56042688 None:intergenic 35.0%
GAATCTGCGAGTGATGAAAA+TGG + chr5_3:56037175-56037194 None:intergenic 40.0%
! GTTGAAGAAGCGTAGATTGA+TGG + chr5_3:56037200-56037219 None:intergenic 40.0%
! TTAGGAGTGAGTTGAAGAAC+GGG + chr5_3:56037308-56037327 None:intergenic 40.0%
! GTTAGGAGTGAGTTGAAGAA+CGG + chr5_3:56037309-56037328 None:intergenic 40.0%
TCCTAACAACTTCAAGTCCA+AGG - chr5_3:56037322-56037341 Msa0819540:three_prime_UTR 40.0%
ACCTTGGACTTGAAGTTGTT+AGG + chr5_3:56037326-56037345 None:intergenic 40.0%
AAAAAGTGGGATCACGAACT+GGG + chr5_3:56037375-56037394 None:intergenic 40.0%
AAAAAAGTGGGATCACGAAC+TGG + chr5_3:56037376-56037395 None:intergenic 40.0%
CCGACACACATTTGTTTACA+TGG - chr5_3:56037773-56037792 Msa0819540:CDS 40.0%
GTTGTGTATGTTTCGTTCTG+TGG - chr5_3:56037877-56037896 Msa0819540:CDS 40.0%
! ATTCGTATTTTCTTGGGGAG+GGG + chr5_3:56038603-56038622 None:intergenic 40.0%
! TAAGGCTCTGACTCCTATAT+GGG - chr5_3:56038766-56038785 Msa0819540:intron 40.0%
GAAGGCAGCTACTGTATTAA+AGG - chr5_3:56038790-56038809 Msa0819540:intron 40.0%
! TACATACCTTTGATGGACCA+CGG - chr5_3:56039035-56039054 Msa0819540:intron 40.0%
ACTTTCCCTAACTGTCTAAC+AGG + chr5_3:56039126-56039145 None:intergenic 40.0%
GAAGACTAGGAATGTAACGA+AGG + chr5_3:56039474-56039493 None:intergenic 40.0%
ACAAATGTCATGTAAGCGAC+AGG + chr5_3:56039571-56039590 None:intergenic 40.0%
GGTGCAAACACCTTTATAGT+TGG + chr5_3:56039777-56039796 None:intergenic 40.0%
!! ACTATAAAGGTGTTTGCACC+TGG - chr5_3:56039777-56039796 Msa0819540:intron 40.0%
! TAATCAACTGGTGGTTTTCC+AGG + chr5_3:56039798-56039817 None:intergenic 40.0%
AAACCACCAGTTGATTACCA+AGG - chr5_3:56039801-56039820 Msa0819540:intron 40.0%
!! TGCGATTGGTTTAACATCTC+TGG + chr5_3:56039829-56039848 None:intergenic 40.0%
TGAAGTGCAAATTCTGCGAT+TGG + chr5_3:56039843-56039862 None:intergenic 40.0%
TTGCACTTCAACAGGTTTGT+TGG - chr5_3:56039853-56039872 Msa0819540:intron 40.0%
ACTCTTCTTGCAACATCACT+TGG - chr5_3:56040067-56040086 Msa0819540:CDS 40.0%
! ACAGGTAAAGGCTCTTTTGA+AGG - chr5_3:56040198-56040217 Msa0819540:intron 40.0%
CGGTTAAATGGAAAAGCAAC+AGG - chr5_3:56040223-56040242 Msa0819540:intron 40.0%
! TAGTTCTACCGAAGAAGAAG+CGG + chr5_3:56040275-56040294 None:intergenic 40.0%
ATGCTGACAAGGTAAGCTTA+TGG - chr5_3:56040569-56040588 Msa0819540:intron 40.0%
TGCTATAGTGCTTCTATAGC+TGG + chr5_3:56040728-56040747 None:intergenic 40.0%
TTTGTCAGTCTCTAATGAGC+AGG - chr5_3:56040904-56040923 Msa0819540:intron 40.0%
ATGAGCAGGTTCAATGTTCA+AGG - chr5_3:56040918-56040937 Msa0819540:intron 40.0%
TCAAGGATTCCCAACAATCT+TGG - chr5_3:56040935-56040954 Msa0819540:intron 40.0%
GCTCCAAATACCAAGATTGT+TGG + chr5_3:56040948-56040967 None:intergenic 40.0%
AGATACTCCAATTCCTTACG+AGG - chr5_3:56040974-56040993 Msa0819540:intron 40.0%
GATACTCCAATTCCTTACGA+GGG - chr5_3:56040975-56040994 Msa0819540:intron 40.0%
ATCTAGTGCGAATGATTCGA+TGG + chr5_3:56041018-56041037 None:intergenic 40.0%
! CTATATAGCTCTGTCACTTC+TGG + chr5_3:56041065-56041084 None:intergenic 40.0%
CAGTTGCAGAGAAGTTCAAA+AGG - chr5_3:56041290-56041309 Msa0819540:intron 40.0%
AGTTCAAAAGGAGTCCTTAC+AGG - chr5_3:56041302-56041321 Msa0819540:intron 40.0%
GGTTGCAAAGTGTACTTACA+TGG - chr5_3:56041554-56041573 Msa0819540:intron 40.0%
GATGCATTGTTGGTGCAAAA+TGG + chr5_3:56041616-56041635 None:intergenic 40.0%
GGTCTTGAACAGCTATGTAT+GGG - chr5_3:56041698-56041717 Msa0819540:intron 40.0%
! GTAGCCTTGAACGTTAAGAA+AGG - chr5_3:56041789-56041808 Msa0819540:intron 40.0%
!! AGTTCAAAAGCACTCTTGAG+AGG + chr5_3:56041825-56041844 None:intergenic 40.0%
GGTTCTGTCTTTACAATGGT+GGG + chr5_3:56042010-56042029 None:intergenic 40.0%
! TGGTTCTGTCTTTACAATGG+TGG + chr5_3:56042011-56042030 None:intergenic 40.0%
! CCATGGTTCTGTCTTTACAA+TGG + chr5_3:56042014-56042033 None:intergenic 40.0%
CCATTGTAAAGACAGAACCA+TGG - chr5_3:56042011-56042030 Msa0819540:intron 40.0%
! AAGACTAGTATAGTGATGGG+TGG - chr5_3:56042277-56042296 Msa0819540:intron 40.0%
! AACATGCCAACATTTTCCTG+AGG + chr5_3:56042348-56042367 None:intergenic 40.0%
TGTTGAATGTTGCACACAAC+CGG + chr5_3:56042460-56042479 None:intergenic 40.0%
GTGGCTTGAAACTCGTATTA+AGG - chr5_3:56042528-56042547 Msa0819540:intron 40.0%
TCGAACGTTGCATCATAAAG+GGG + chr5_3:56042587-56042606 None:intergenic 40.0%
CTCGAACGTTGCATCATAAA+GGG + chr5_3:56042588-56042607 None:intergenic 40.0%
CAACGTTCGAGCTATGTTTA+CGG - chr5_3:56042597-56042616 Msa0819540:CDS 40.0%
ATCCTTTCTCACGCTCTCTA+CGG - chr5_3:56037272-56037291 Msa0819540:three_prime_UTR 45.0%
!!! CGTGATCCCACTTTTTTAGC+TGG - chr5_3:56037379-56037398 Msa0819540:three_prime_UTR 45.0%
GTCAGTGTCCATGTCGTATT+AGG - chr5_3:56037835-56037854 Msa0819540:CDS 45.0%
CATGGACACCTAATACGACA+TGG + chr5_3:56037846-56037865 None:intergenic 45.0%
GAATTCTTTGCTCCATGGTG+CGG - chr5_3:56038737-56038756 Msa0819540:intron 45.0%
AATTCTTTGCTCCATGGTGC+GGG - chr5_3:56038738-56038757 Msa0819540:intron 45.0%
! GTAAGGCTCTGACTCCTATA+TGG - chr5_3:56038765-56038784 Msa0819540:intron 45.0%
TCTGACTCCTATATGGGAGA+AGG - chr5_3:56038772-56038791 Msa0819540:intron 45.0%
GTAGAGCATGGTAGGATCTT+GGG + chr5_3:56039421-56039440 None:intergenic 45.0%
TGTAGAGCATGGTAGGATCT+TGG + chr5_3:56039422-56039441 None:intergenic 45.0%
! GTTTAACATCTCTGGCTCCT+TGG + chr5_3:56039821-56039840 None:intergenic 45.0%
CGCAGAATTTGCACTTCAAC+AGG - chr5_3:56039845-56039864 Msa0819540:intron 45.0%
AGAAACCACCGCTTCTTCTT+CGG - chr5_3:56040264-56040283 Msa0819540:intron 45.0%
! TTGGATGCTCTTTTCCACTC+AGG + chr5_3:56040508-56040527 None:intergenic 45.0%
! TGTTGACTGTGATGCTGACA+AGG - chr5_3:56040558-56040577 Msa0819540:intron 45.0%
! GAAGCACTATAGCACTGTAG+TGG - chr5_3:56040734-56040753 Msa0819540:intron 45.0%
ATCATTCGCACTAGATCAGC+TGG - chr5_3:56041022-56041041 Msa0819540:intron 45.0%
! TATAGCTCTGTCACTTCTGG+AGG + chr5_3:56041062-56041081 None:intergenic 45.0%
! TTCCTTCCTTCCCGACATTT+TGG - chr5_3:56041222-56041241 Msa0819540:intron 45.0%
ATCCAAAATGTCGGGAAGGA+AGG + chr5_3:56041227-56041246 None:intergenic 45.0%
TGGAATCCAAAATGTCGGGA+AGG + chr5_3:56041231-56041250 None:intergenic 45.0%
! TCCCGACATTTTGGATTCCA+AGG - chr5_3:56041231-56041250 Msa0819540:intron 45.0%
GCCTTGGAATCCAAAATGTC+GGG + chr5_3:56041235-56041254 None:intergenic 45.0%
CGCCTTGGAATCCAAAATGT+CGG + chr5_3:56041236-56041255 None:intergenic 45.0%
! CGACATTTTGGATTCCAAGG+CGG - chr5_3:56041234-56041253 Msa0819540:intron 45.0%
! ATTTTGGATTCCAAGGCGGA+GGG - chr5_3:56041238-56041257 Msa0819540:intron 45.0%
GGGTCTTGAACAGCTATGTA+TGG - chr5_3:56041697-56041716 Msa0819540:intron 45.0%
CAACACCAACTTTCTGCTCA+AGG + chr5_3:56041746-56041765 None:intergenic 45.0%
!! CTTGAGCAGAAAGTTGGTGT+TGG - chr5_3:56041744-56041763 Msa0819540:intron 45.0%
!! AGCAGAAAGTTGGTGTTGGT+GGG - chr5_3:56041748-56041767 Msa0819540:intron 45.0%
!! AAAGTTGGTGTTGGTGGGTA+TGG - chr5_3:56041753-56041772 Msa0819540:intron 45.0%
ACGTTCAAGGCTACTAATGC+TGG + chr5_3:56041783-56041802 None:intergenic 45.0%
TTTGTGAAAGAAGCTGGACG+TGG - chr5_3:56041950-56041969 Msa0819540:intron 45.0%
! TCTGTCTTTACAATGGTGGG+AGG + chr5_3:56042007-56042026 None:intergenic 45.0%
GTAAAGACAGAACCATGGGA+TGG - chr5_3:56042016-56042035 Msa0819540:intron 45.0%
GAACCATGGGATGGAAAAGA+TGG - chr5_3:56042025-56042044 Msa0819540:intron 45.0%
! TCTCCATCTTTTCCATCCCA+TGG + chr5_3:56042031-56042050 None:intergenic 45.0%
!!! TAGTGATGGGTGGTCTTTTG+AGG - chr5_3:56042287-56042306 Msa0819540:intron 45.0%
TCAGTGGTACAACATACCTC+AGG - chr5_3:56042329-56042348 Msa0819540:intron 45.0%
! TACGAGAAGACCTGGTTCTT+TGG - chr5_3:56042413-56042432 Msa0819540:intron 45.0%
GCTCGAACGTTGCATCATAA+AGG + chr5_3:56042589-56042608 None:intergenic 45.0%
! AACCGTAGAGAGCGTGAGAA+AGG + chr5_3:56037277-56037296 None:intergenic 50.0%
! AGTGAGTTGAAGAACGGGTG+AGG + chr5_3:56037303-56037322 None:intergenic 50.0%
!! CATAAAGCACCGGCACTCTT+CGG - chr5_3:56037739-56037758 Msa0819540:three_prime_UTR 50.0%
ACGACATGGACACTGACATG+TGG + chr5_3:56037832-56037851 None:intergenic 50.0%
GTAGCTGCCTTCTCCCATAT+AGG + chr5_3:56038782-56038801 None:intergenic 50.0%
TGCTGATGCTCACCAATCTC+TGG - chr5_3:56038838-56038857 Msa0819540:CDS 50.0%
GGAAAACCTGAGCCAGAGAT+TGG + chr5_3:56038853-56038872 None:intergenic 50.0%
ACCACGGACGATGCAAAACA+TGG - chr5_3:56039051-56039070 Msa0819540:intron 50.0%
! ACCATGTTTTGCATCGTCCG+TGG + chr5_3:56039055-56039074 None:intergenic 50.0%
GGAGGACACTTGACCCTATT+GGG + chr5_3:56039307-56039326 None:intergenic 50.0%
AGGAGGACACTTGACCCTAT+TGG + chr5_3:56039308-56039327 None:intergenic 50.0%
! GCTCCTTGGTAATCAACTGG+TGG + chr5_3:56039807-56039826 None:intergenic 50.0%
! CTGGCTCCTTGGTAATCAAC+TGG + chr5_3:56039810-56039829 None:intergenic 50.0%
TTCTACCGAAGAAGAAGCGG+TGG + chr5_3:56040272-56040291 None:intergenic 50.0%
CTAGCACCCTCGTAAGGAAT+TGG + chr5_3:56040984-56041003 None:intergenic 50.0%
GCAGGAGAACAAACACTCAC+TGG + chr5_3:56041089-56041108 None:intergenic 50.0%
! CATTTTGGATTCCAAGGCGG+AGG - chr5_3:56041237-56041256 Msa0819540:intron 50.0%
! TTTTGGATTCCAAGGCGGAG+GGG - chr5_3:56041239-56041258 Msa0819540:intron 50.0%
CCAACTTTCTGCTCAAGGTC+TGG + chr5_3:56041741-56041760 None:intergenic 50.0%
CCAGACCTTGAGCAGAAAGT+TGG - chr5_3:56041738-56041757 Msa0819540:intron 50.0%
!! GAGCAGAAAGTTGGTGTTGG+TGG - chr5_3:56041747-56041766 Msa0819540:intron 50.0%
GAGGAACGATCCAAAGAACC+AGG + chr5_3:56042426-56042445 None:intergenic 50.0%
CGTTCCTCATTGACCATAGC+CGG - chr5_3:56042438-56042457 Msa0819540:intron 50.0%
ACAACCGGCTATGGTCAATG+AGG + chr5_3:56042445-56042464 None:intergenic 50.0%
ATGTTGCACACAACCGGCTA+TGG + chr5_3:56042454-56042473 None:intergenic 50.0%
!! CGAAGAGTGCCGGTGCTTTA+TGG + chr5_3:56037741-56037760 None:intergenic 55.0%
! ACGCCTAATCCGAAGAGTGC+CGG + chr5_3:56037751-56037770 None:intergenic 55.0%
! TGCTCACCAATCTCTGGCTC+AGG - chr5_3:56038844-56038863 Msa0819540:CDS 55.0%
CATCGTCCGTGGTCCATCAA+AGG + chr5_3:56039044-56039063 None:intergenic 55.0%
! GCAGTTCTAGCACCCTCGTA+AGG + chr5_3:56040990-56041009 None:intergenic 55.0%
AGCTATGTATGGGCAGCTGC+CGG - chr5_3:56041708-56041727 Msa0819540:intron 55.0%
CTCAAGGTCTGGCTGCTTAC+CGG + chr5_3:56041730-56041749 None:intergenic 55.0%
GTGAAAGAAGCTGGACGTGG+AGG - chr5_3:56041953-56041972 Msa0819540:intron 55.0%
AAGCTGGACGTGGAGGCAAA+GGG - chr5_3:56041960-56041979 Msa0819540:intron 55.0%
! GCACCGGCACTCTTCGGATT+AGG - chr5_3:56037745-56037764 Msa0819540:three_prime_UTR 60.0%
! TCCATGGTGCGGGCACTGTA+AGG - chr5_3:56038748-56038767 Msa0819540:intron 60.0%
! TCTGTTTCTCCCCTCCGCCT+TGG + chr5_3:56041251-56041270 None:intergenic 60.0%
GAAGCTGGACGTGGAGGCAA+AGG - chr5_3:56041959-56041978 Msa0819540:intron 60.0%
GGCAAAGGGAACTTGCCCCT+AGG - chr5_3:56041974-56041993 Msa0819540:intron 60.0%
!! GTGGGAGGAGTGTCACCTAG+GGG + chr5_3:56041992-56042011 None:intergenic 60.0%
!! GGTGGGAGGAGTGTCACCTA+GGG + chr5_3:56041993-56042012 None:intergenic 60.0%
!! TGGTGGGAGGAGTGTCACCT+AGG + chr5_3:56041994-56042013 None:intergenic 60.0%
GCCTTACAGTGCCCGCACCA+TGG + chr5_3:56038752-56038771 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 56037137 56042757 56037137 ID=Msa0819540;Name=Msa0819540
chr5_3 mRNA 56037137 56042757 56037137 ID=Msa0819540-mRNA-1;Parent=Msa0819540;Name=Msa0819540-mRNA-1;_AED=0.18;_eAED=0.18;_QI=84|1|1|1|1|1|9|634|435
chr5_3 exon 56037137 56037948 56037137 ID=Msa0819540-mRNA-1:exon:16978;Parent=Msa0819540-mRNA-1
chr5_3 exon 56038038 56038184 56038038 ID=Msa0819540-mRNA-1:exon:16977;Parent=Msa0819540-mRNA-1
chr5_3 exon 56038571 56038716 56038571 ID=Msa0819540-mRNA-1:exon:16976;Parent=Msa0819540-mRNA-1
chr5_3 exon 56038806 56038982 56038806 ID=Msa0819540-mRNA-1:exon:16975;Parent=Msa0819540-mRNA-1
chr5_3 exon 56039315 56039414 56039315 ID=Msa0819540-mRNA-1:exon:16974;Parent=Msa0819540-mRNA-1
chr5_3 exon 56039529 56039692 56039529 ID=Msa0819540-mRNA-1:exon:16973;Parent=Msa0819540-mRNA-1
chr5_3 exon 56040028 56040141 56040028 ID=Msa0819540-mRNA-1:exon:16972;Parent=Msa0819540-mRNA-1
chr5_3 exon 56041029 56041187 56041029 ID=Msa0819540-mRNA-1:exon:16971;Parent=Msa0819540-mRNA-1
chr5_3 exon 56042551 56042757 56042551 ID=Msa0819540-mRNA-1:exon:16970;Parent=Msa0819540-mRNA-1
chr5_3 five_prime_UTR 56042674 56042757 56042674 ID=Msa0819540-mRNA-1:five_prime_utr;Parent=Msa0819540-mRNA-1
chr5_3 CDS 56042551 56042673 56042551 ID=Msa0819540-mRNA-1:cds;Parent=Msa0819540-mRNA-1
chr5_3 CDS 56041029 56041187 56041029 ID=Msa0819540-mRNA-1:cds;Parent=Msa0819540-mRNA-1
chr5_3 CDS 56040028 56040141 56040028 ID=Msa0819540-mRNA-1:cds;Parent=Msa0819540-mRNA-1
chr5_3 CDS 56039529 56039692 56039529 ID=Msa0819540-mRNA-1:cds;Parent=Msa0819540-mRNA-1
chr5_3 CDS 56039315 56039414 56039315 ID=Msa0819540-mRNA-1:cds;Parent=Msa0819540-mRNA-1
chr5_3 CDS 56038806 56038982 56038806 ID=Msa0819540-mRNA-1:cds;Parent=Msa0819540-mRNA-1
chr5_3 CDS 56038571 56038716 56038571 ID=Msa0819540-mRNA-1:cds;Parent=Msa0819540-mRNA-1
chr5_3 CDS 56038038 56038184 56038038 ID=Msa0819540-mRNA-1:cds;Parent=Msa0819540-mRNA-1
chr5_3 CDS 56037771 56037948 56037771 ID=Msa0819540-mRNA-1:cds;Parent=Msa0819540-mRNA-1
chr5_3 three_prime_UTR 56037137 56037770 56037137 ID=Msa0819540-mRNA-1:three_prime_utr;Parent=Msa0819540-mRNA-1
Gene Sequence

>Msa0819540

ATGGCGAAACTCTCATTCATTGCTGTAACTATCTTTCTCTTCAACAATCTCATCCTTTCTCACGCTCTCTACGGTTCTTCCTCACCCGTTCTTCAACTCACTCCTAACAACTTCAAGTCCAAGGTTCTGAATTCAAATGGAGTTGTTCTTGTTGAATTCTTTGCTCCATGGTGCGGGCACTGTAAGGCTCTGACTCCTATATGGGAGAAGGCAGCTACTGTATTAAAGGGTGTAGTTACTGTTGCAGCACTTGATGCTGATGCTCACCAATCTCTGGCTCAGGAATATGGTATTAGAGGATTTCCAACTATAAAGGTGTTTGCACCTGGAAAACCACCAGTTGATTACCAAGGAGCCAGAGATGTTAAACCAATCGCAGAATTTGCACTTCAACAGGTAAAGGCTCTTTTGAAGGAACGGTTAAATGGAAAAGCAACAGGTGGATCAAATGAAAAAAAAGAAACCACCGCTTCTTCTTCGGTAGAACTAAACTCTAGTAACTTCGATGAATTGGTTATCAAAAGCAAAGAACTTTGGATTGTAGAATTTTTCGCACCTTGGTGTGGTCATTGTAAAAAATTAGCTCCTGAGTGGAAAAGAGCATCCAATAATTTGAAAGGGAAGGTTAAACTGGGTCATGTTGACTGTGATGCTGACAAGTCTCTAATGAGCAGGTTCAATGTTCAAGGATTCCCAACAATCTTGGTATTTGGAGCCGATAAAGATACTCCAATTCCTTACGAGGGTGCTAGAACTGCATCAGCCATCGAATCATTCGCACTAGATCAGCTGGAAACAAATGTTGCTCCTCCAGAAGTGACAGAGCTATATAGTCCAGATGTTTTGGAAGAGAAATGTGGTTCTGCTGCTATCTGTTTTGTTTCCTTCCTTCCCGACATTTTGGATTCCAAGGCGGAGGGGAGAAACAGATACCTTCAGCAGTTATTAACAGTTGCAGAGAAGTTCAAAAGGAGTCCTTACAGCTATGTATGGGCAGCTGCCGGTAAGCAGCCAGACCTTGAGCAGAAAGTTGGTGTTGGTGGGTATGGTTATCCAGCATTAGTAGCCTTGAACGTTAAGAAAGGTGTTTATGCTCCTCTCAAGAGTGCTTTTGAACTTGACCAAATTATAGAATTTGTGAAAGAAGCTGGACGTGGAGGCAAAGGGAACTTGCCCCTAGGTGACACTCCTCCCACCATTGTAAAGACAGAACCATGGGATGGAAAAGATGGAGAAATAATTGAGGAAGATGAATTTTCTCTTGAAGAACTCATGGGAGAAGATGCCTCAACCAAAGATGAACTATAA
Protein sequence

>Msa0819540

MAKLSFIAVTIFLFNNLILSHALYGSSSPVLQLTPNNFKSKVLNSNGVVLVEFFAPWCGHCKALTPIWEKAATVLKGVVTVAALDADAHQSLAQEYGIRGFPTIKVFAPGKPPVDYQGARDVKPIAEFALQQVKALLKERLNGKATGGSNEKKETTASSSVELNSSNFDELVIKSKELWIVEFFAPWCGHCKKLAPEWKRASNNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPIPYEGARTASAIESFALDQLETNVAPPEVTELYSPDVLEEKCGSAAICFVSFLPDILDSKAEGRNRYLQQLLTVAEKFKRSPYSYVWAAAGKQPDLEQKVGVGGYGYPALVALNVKKGVYAPLKSAFELDQIIEFVKEAGRGGKGNLPLGDTPPTIVKTEPWDGKDGEIIEEDEFSLEELMGEDASTKDEL*